Rroxscaffold_7G00203630

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011365
Physical Location & Seq
Reverse (-)
52527268 .. 52534110
6843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00203630.1

Sequence Viewer

Length: 2625 bp
ATGGCTCAGCCTCTCGTGAAGAAAGACGACGATCGCGACGATGAAGGTAATGTCCGTTTTTCTGTTAGGCAAATCAATTTTCACCGCCCTCAAATTCGATCTGGTTTTTTCCGCTCCCAATCTTGCATACTGTTTCGGTGCTCTGAGTACTCTCCATTTCTGGGGATTGAAAAGGGGGCTGTTCTTCAAGAAGCTAGGGTCTTCAATGATCCTCAGCTAGACGCAAGAAGATGCTCTCAGGTTATCACAAAGCTTCTGTATCTACTGAATCAGGGTGAAACCTTCACTAAGGTTGAAGCTACAGAAGTTTTCTTTTCGGTGACCAAGCTTTTCCAGTCGAGAGATATAGGATTGAGGAGAATGGTTTATCTGATGATAAAAGAGTTATCGCCTTCTGCTGATGAGGTTATTATTGTGACAAGCTCCCTAATGAAGGACATGAATAGTAAAACTGATATGTATCGGGCCAATGCCATTCGTGTGCTTTGTCGGATTACGGATGGAACCCTTCTTACCCAGATTGAGCGGTATTTGAAACAAGCCATTGTTGACAAGAATCCAGTGGTTGCAAGTGCAGCTCTAGTTAGTGGGATCCATCTACTTCAGACTAACCCAGAGATTGTTAAAAGGTGGAGTAATGAGGTTCAGGAAGCTGTCCAGTCAAGGGCAGCCCTTGTACAGTTTCATGCACTGGCTTTACTTCATCAGATAAGACAAAATGACCGTCTTGCTGTTAGCAAGTTGGTTACCAGCTTGACAAGGGGCACTGTCCGTTCTCCATTAGCTCAATGCCTGTTGATCCGCTATACTAGTCAGGTAATCCGGGAGTCTGCTGGGAGTACACAAGCAGGTGACCGTCCTTTTTATGATTATCTCGAGGGTTGCCTGCGGCACAAAGCAGAGATGGTAATTTTTGAAGCTGCCAGAGCAATTACAGAGCTCCATGGTGTGACCAACAGAGAATTGACTCCAGCTATCACCGTTCTTCAGCTATTTTTAAGTTCTTCAAAGCCAGTGTTAAGATTTGCTGCGGTCCGCACCCTAAACAAGGTGGCGATGACTCATCCAATGGCCGTCACAAACTGCAACATTGATATGGAGAGTTTGATTTCTGACCAGAACAGGAGCATTGCCACACTTGCGATCACCACACTTCTGAAGACAGGAAATGAATCGAGTGTGGATCGCTTGATGAAGCAGATAACTAACTTCATGTCAGATATTGCTGATGAATTCAAAATTGTAGTTGTGGAAGCCATTAGATCTCTGTGTTTGAAGTTTCCATTGAAGTATAGAGCTCTCATGAACTTCCTAAGCAACATTCTCCGAGAAGAAGGCGGGTTTGAGTACAAAAAAGCAATTGTTGACTCAATTGTAATTCTCATTAGAGATATCCCAGAAGCAAAAGAAAGTGGCTTGCTTCATCTCTGTGAGTTCATTGAAGATTGTGAATTTACCTATCTTTCTACTCAGATACTCCACTTTTTGGGGATTGAAGGGCCAAGAACCTCGGATCCAAGCAAATACATACGGTACATTTATAATCGAGTGCATCTTGAGAATGCCACTGTCCGGGCCAGTGCTGTGAGCACATTAGCCAAATTTGGTGCCATGGTTGATTCATTGAAGGTTCGTGATAGGGCAACACTCTATCTTAGTACACTTGGAGGGGATGGCTGTGTTGTTGAGACTGACCAGGATGTAAAGGACTTTCTCTTTGGGTCTCTGGATGTCCCCCTAGTTAATCTTGAGACAAGTTTGAAGAATTATGAGGCTTCAGAAGAACCATTTGACATCAATTCTGTACCCAAGGAGATCAAGTCTCAGCCACTTGCTGAGAAAAAGCCCAGAGTAAAAAGCCAACTGGTCTGGGTGCTCCACCAATCCTCAGCACCTGTGGAGTTGACAGAGGCAGAAACTGAATATGCAGTTAACGTTGTCAAGCACATTTTTGATAGGCATGTTGTGTTCCAGTACAACTGCACCAACACCATTCCCGAGCAATTACTGGAAAATGTTATTGTTGCCGTTGATGCTTCTGATGCAGAGGAGTTTACTGAAGTGGGATCCAAGCCTCTCCGGTCTCTTCCTTATGATACACCTGGCCAGACCTTTTTGGCATTTGAGAAGCCAGAAGGTGTCCTTGCAGTTGGAAAATTTTCAAACACATTGAGATTCATTGTTAAAGAGGTTGATCCAACCACTGGAGAGGCAGAGGAAGATGGTGTAGAAGATGAATACCAGCTGGAGGACCTTGAGGTGGTTGCTGCTGATTATATTTTGAAGGATCAAGTACTCAATTTCAGAAATGCATGGGAAACCATGGGCCCAGATTGTGAGCGTGTAGATGAATATGGCCTGGGCCAAAGAGAGAGCTTGAATGAAGCAGTCTCTGCTGTCATCAGCCTCCTTGGAATGCAGCCCTGTGAGGGCACGGAGGTTATTGCCAGCAACTCAAGGTCCCACACTTGCTTATTGGCTGGTGTATACATAGGCAATGTGAAGGTCCTCGTGCGGTTGTCTTTTGGAATTGATAGTTCAAAGGAGGTTGCGATGAAGCTTGTGGTTAGATCTGAGGATGTGACTGTCAGCGATGCCATCCATGAGATTGTGGCCAGCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N COP-gamma_platf Coatomer_g_Cpla

Protein Analysis

874

Amino Acids

97.81

Weight (kDa)

5.39

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 59 - 549 4.8e-135 Adaptin N terminal region
Cnd1 PF12717 155 - 250 1.5e-06 non-SMC mitotic condensation complex subunit 1
COP-gamma_platf PF08752 626 - 754 1.7e-46 Coatomer gamma subunit appendage platform subdomain
Coatomer_g_Cpla PF16381 757 - 871 1.4e-34 Coatomer subunit gamma-1 C-terminal appendage platform
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34450
fragaria_vesca FvH4_2g08660 FvH4_2g08660 FvH4_2g08660
malus_domestica MD05G1067500.v1.1 MD10G1077400.v1.1
prunus_persica Prupe.8G098200_v2.0.a1
pyrus_communis pycom05g05860 pycom10g06040
rosa_chinensis RchiOBHm_Chr6g0264051
rosa_laevigata RLG00000014293
rosa_multiflora Rmu_sc0006339.1_g000005
rosa_roxburghii Rroxscaffold_176G00431390 Rroxscaffold_7G00203630
rosa_rugosa Rorug06G0007600
rosa_samantha Rh6AG128800 Rh6BG124900 Rh6CG123600 Rh6DG110600
rosa_wichuraiana Rw6G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1542
AarI CACCTGC 1 cut(s) 839
Acc36I ACCTGC 1 cut(s) 839
AccB1I GGYRCC 1 cut(s) 1607
AccB7I CCANNNNNTGG 2 cut(s) 1486, 2204
AccBSI CCGCTC 2 cut(s) 114, 526
AccI GTMKAC 1 cut(s) 2487
AccII CGCG 1 cut(s) 36
AclI AACGTT 1 cut(s) 1935
AcoI YGGCCR 3 cut(s) 1071, 2104, 2613
AcsI RAATTY 5 cut(s) 93, 1232, 1451, 1601, 2156
AcuI CTGAAG 5 cut(s) 587, 971, 1178, 1761, 2079
AfaI GTAC 9 cut(s) 149, 678, 841, 1349, 1535, 1660, 1806, 1976, 2295
AhlI ACTAGT 1 cut(s) 809
AjnI CCWGG 3 cut(s) 1695, 2101, 2358
AloI GAACNNNNNNTCC 2 cut(s) 1498, 1530
Alw21I GWGCWC 5 cut(s) 143, 942, 1300, 1592, 1878
Alw26I GTCTC 6 cut(s) 1682, 1728, 1745, 1827, 2088, 2395
AlwNI CAGNNNCTG 3 cut(s) 1895, 1919, 2393
Ama87I CYCGRG 2 cut(s) 875, 1997
AoxI GGCC 9 cut(s) 465, 1071, 1499, 1575, 2104, 2326, 2356, 2362, 2613
ApaI GGGCCC 1 cut(s) 2330
ApeKI GCWGC 6 cut(s) 575, 668, 920, 1028, 2267, 2419
ApoI RAATTY 5 cut(s) 93, 1232, 1451, 1601, 2156
ArsI GACNNNNNNTTYG 4 cut(s) 709, 741, 1700, 1732
Asp700I GAANNNNTTC 1 cut(s) 2158
AsuC2I CCSGG 2 cut(s) 824, 1574
AsuHPI GGTGA 6 cut(s) 74, 287, 331, 863, 970, 1138
AvaI CYCGRG 2 cut(s) 875, 1997
AvaII GGWCC 4 cut(s) 1033, 2251, 2460, 2506
BaeGI GKGCMC 3 cut(s) 767, 2330, 2435
BalI TGGCCA 2 cut(s) 2106, 2615
BamHI GGATCC 3 cut(s) 591, 1513, 2066
BanI GGYRCC 1 cut(s) 1607
BanII GRGCYC 3 cut(s) 942, 1300, 2330
BauI CACGAG 2 cut(s) 14, 2510
BbsI GAAGAC 2 cut(s) 193, 1166
Bbv12I GWGCWC 5 cut(s) 143, 942, 1300, 1592, 1878
BbvCI CCTCAGC 2 cut(s) 213, 1888
BbvI GCAGC 6 cut(s) 587, 680, 907, 1015, 2254, 2431
BccI CCATC 6 cut(s) 494, 603, 898, 1667, 2216, 2606
BceAI ACGGC 2 cut(s) 1058, 2012
BciT130I CCWGG 3 cut(s) 1697, 2103, 2360
BcnI CCSGG 2 cut(s) 824, 1574
BcoDI GTCTC 6 cut(s) 1682, 1728, 1745, 1827, 2088, 2395
BcuI ACTAGT 1 cut(s) 809
BfaI CTAG 6 cut(s) 195, 218, 581, 810, 1739, 2623
BfmI CTRYAG 1 cut(s) 300
BfuAI ACCTGC 1 cut(s) 839
BglII AGATCT 2 cut(s) 1262, 2570
BisI GCNGC 8 cut(s) 576, 669, 890, 921, 1029, 2268, 2420, 2620
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 8 cut(s) 577, 670, 891, 922, 1030, 2269, 2421, 2621
BmcAI AGTACT 2 cut(s) 149, 2295
Bme1390I CCNGG 5 cut(s) 824, 1574, 1697, 2103, 2360
Bme18I GGWCC 4 cut(s) 1033, 2251, 2460, 2506
BmeT110I CYCGRG 2 cut(s) 875, 1997
BmiI GGNNCC 7 cut(s) 505, 593, 1515, 1609, 2068, 2328, 2462
BmrFI CCNGG 5 cut(s) 824, 1574, 1697, 2103, 2360
BmsI GCATC 5 cut(s) 221, 1561, 2023, 2032, 2584
BpiI GAAGAC 2 cut(s) 193, 1166
BpmI CTGGAG 3 cut(s) 954, 2226, 2267
Bpu10I CCTNAGC 3 cut(s) 213, 1313, 1888
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 4 cut(s) 1577, 1769, 2276, 2440
BpuMI CCSGG 2 cut(s) 824, 1574
BsaBI GATNNNNATC 1 cut(s) 459
BsaI GGTCTC 2 cut(s) 1728, 2088
BsaJI CCNNGG 7 cut(s) 943, 1509, 1611, 1809, 2322, 2359, 2410
BsaWI WCCGGW 1 cut(s) 2079
BsaXI ACNNNNNCTCC 2 cut(s) 1805, 1835
Bse3DI GCAATG 2 cut(s) 1128, 2503
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 3 cut(s) 1697, 2103, 2360
BseDI CCNNGG 7 cut(s) 943, 1509, 1611, 1809, 2322, 2359, 2410
BseGI GGATG 7 cut(s) 505, 1063, 1678, 1705, 1735, 2584, 2598
BseJI GATNNNNATC 1 cut(s) 459
BseMI GCAATG 2 cut(s) 1128, 2503
BseMII CTCAG 9 cut(s) 20, 135, 227, 251, 1484, 1827, 1838, 1902, 2565
BseRI GAGGAG 2 cut(s) 370, 2063
BseSI GKGCMC 3 cut(s) 767, 2330, 2435
BseXI GCAGC 6 cut(s) 587, 680, 907, 1015, 2254, 2431
BseYI CCCAGC 1 cut(s) 833
BsgI GTGCAG 2 cut(s) 594, 1966
Bsh1236I CGCG 1 cut(s) 36
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 9 cut(s) 467, 1073, 1501, 1577, 2106, 2328, 2358, 2364, 2615
BshNI GGYRCC 1 cut(s) 1607
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 5 cut(s) 143, 942, 1300, 1592, 1878
BsiHKCI CYCGRG 2 cut(s) 875, 1997
BsiSI CCGG 3 cut(s) 823, 1573, 2080
BslFI GGGAC 2 cut(s) 1718, 2446
BsmAI GTCTC 6 cut(s) 1682, 1728, 1745, 1827, 2088, 2395
BsmFI GGGAC 2 cut(s) 1718, 2446
BsmI GAATGC 2 cut(s) 1567, 2421
BsnI GGCC 9 cut(s) 467, 1073, 1501, 1577, 2106, 2328, 2358, 2364, 2615
Bso31I GGTCTC 2 cut(s) 1728, 2088
BsoBI CYCGRG 2 cut(s) 875, 1997
Bsp120I GGGCCC 1 cut(s) 2326
Bsp1286I GDGCHC 8 cut(s) 143, 767, 942, 1300, 1592, 1878, 2330, 2435
Bsp1407I TGTACA 1 cut(s) 676
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 3 cut(s) 943, 1611, 2322
Bsp68I TCGCGA 1 cut(s) 36
BspANI GGCC 9 cut(s) 467, 1073, 1501, 1577, 2106, 2328, 2358, 2364, 2615
BspCNI CTCAG 9 cut(s) 19, 136, 226, 250, 1483, 1828, 1837, 1901, 2566
BspFNI CGCG 1 cut(s) 36
BspHI TCATGA 1 cut(s) 1302
BspLI GGNNCC 7 cut(s) 505, 593, 1515, 1609, 2068, 2328, 2462
BspMI ACCTGC 1 cut(s) 839
BspT107I GGYRCC 1 cut(s) 1607
BspTNI GGTCTC 2 cut(s) 1728, 2088
BsrBI CCGCTC 2 cut(s) 114, 526
BsrDI GCAATG 2 cut(s) 1128, 2503
BsrGI TGTACA 1 cut(s) 676
BssECI CCNNGG 7 cut(s) 943, 1509, 1611, 1809, 2322, 2359, 2410
BssNAI GTATAC 1 cut(s) 2488
BssSI CACGAG 2 cut(s) 14, 2510
BssT1I CCWWGG 5 cut(s) 943, 1611, 1809, 2322, 2410
Bst1107I GTATAC 1 cut(s) 2488
Bst2BI CACGAG 2 cut(s) 14, 2510
Bst2UI CCWGG 3 cut(s) 1697, 2103, 2360
Bst4CI ACNGT 9 cut(s) 132, 681, 725, 769, 857, 982, 1533, 1570, 2587
Bst6I CTCTTC 1 cut(s) 2091
BstAPI GCANNNNNTGC 1 cut(s) 2393
BstAUI TGTACA 1 cut(s) 676
BstC8I GCNNGC 4 cut(s) 887, 1418, 2449, 2617
BstDSI CCRYGG 3 cut(s) 943, 1611, 2322
BstEII GGTNACC 3 cut(s) 319, 745, 851
BstENI CCTNNNNNAGG 2 cut(s) 431, 1046
BstF5I GGATG 7 cut(s) 505, 1063, 1678, 1705, 1735, 2584, 2598
BstFNI CGCG 1 cut(s) 36
BstMAI GTCTC 6 cut(s) 1682, 1728, 1745, 1827, 2088, 2395
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 6 cut(s) 575, 926, 1139, 2033, 2042, 2393
BstNI CCWGG 3 cut(s) 1697, 2103, 2360
BstNSI RCATGY 1 cut(s) 1964
BstPI GGTNACC 3 cut(s) 319, 745, 851
BstSCI CCNGG 5 cut(s) 822, 1572, 1695, 2101, 2358
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 3 cut(s) 767, 2330, 2435
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 6 cut(s) 587, 680, 907, 1015, 2254, 2431
BstV2I GAAGAC 2 cut(s) 193, 1166
BstX2I RGATCY 5 cut(s) 591, 1262, 1513, 2066, 2570
BstYI RGATCY 5 cut(s) 591, 1262, 1513, 2066, 2570
BstZ17I GTATAC 1 cut(s) 2488
BsuRI GGCC 9 cut(s) 467, 1073, 1501, 1577, 2106, 2328, 2358, 2364, 2615
BtgI CCRYGG 3 cut(s) 943, 1611, 2322
BtgZI GCGATG 3 cut(s) 1070, 2567, 2607
BtsCI GGATG 7 cut(s) 505, 1063, 1678, 1705, 1735, 2584, 2598
BtsIMutI CAGTG 7 cut(s) 567, 689, 765, 1020, 1566, 1585, 2202
BtuMI TCGCGA 1 cut(s) 36
BveI ACCTGC 1 cut(s) 839
Cac8I GCNNGC 4 cut(s) 887, 1418, 2449, 2617
CaiI CAGNNNCTG 3 cut(s) 1895, 1919, 2393
CciI TCATGA 1 cut(s) 1302
CpoI CGGWCCG 1 cut(s) 1033
CseI GACGC 1 cut(s) 230
Csp6I GTAC 9 cut(s) 148, 677, 840, 1348, 1534, 1659, 1805, 1975, 2294
CspI CGGWCCG 1 cut(s) 1033
CviQI GTAC 9 cut(s) 148, 677, 840, 1348, 1534, 1659, 1805, 1975, 2294
EaeI YGGCCR 3 cut(s) 1071, 2104, 2613
Eam1104I CTCTTC 1 cut(s) 2091
EarI CTCTTC 1 cut(s) 2091
Ecl136II GAGCTC 2 cut(s) 940, 1298
Eco130I CCWWGG 5 cut(s) 943, 1611, 1809, 2322, 2410
Eco24I GRGCYC 3 cut(s) 942, 1300, 2330
Eco31I GGTCTC 2 cut(s) 1728, 2088
Eco32I GATATC 1 cut(s) 1393
Eco47I GGWCC 4 cut(s) 1033, 2251, 2460, 2506
Eco53kI GAGCTC 2 cut(s) 940, 1298
Eco57I CTGAAG 5 cut(s) 587, 971, 1178, 1761, 2079
Eco88I CYCGRG 2 cut(s) 875, 1997
Eco91I GGTNACC 3 cut(s) 319, 745, 851
EcoICRI GAGCTC 2 cut(s) 940, 1298
EcoNI CCTNNNNNAGG 2 cut(s) 431, 1046
EcoO109I RGGNCCY 3 cut(s) 2251, 2460, 2506
EcoO65I GGTNACC 3 cut(s) 319, 745, 851
EcoRI GAATTC 1 cut(s) 1232
EcoRII CCWGG 3 cut(s) 1695, 2101, 2358
EcoRV GATATC 1 cut(s) 1393
EcoT14I CCWWGG 5 cut(s) 943, 1611, 1809, 2322, 2410
EcoT22I ATGCAT 1 cut(s) 2314
EcoT38I GRGCYC 3 cut(s) 942, 1300, 2330
ErhI CCWWGG 5 cut(s) 943, 1611, 1809, 2322, 2410
FalI AAGNNNNNCTT 2 cut(s) 2127, 2159
FaqI GGGAC 2 cut(s) 1718, 2446
FauI CCCGC 1 cut(s) 1331
FblI GTMKAC 1 cut(s) 2487
Fnu4HI GCNGC 8 cut(s) 576, 669, 890, 921, 1029, 2268, 2420, 2620
FokI GGATG 7 cut(s) 512, 1050, 1685, 1712, 1742, 2585, 2591
FriOI GRGCYC 3 cut(s) 942, 1300, 2330
Fsp4HI GCNGC 8 cut(s) 576, 669, 890, 921, 1029, 2268, 2420, 2620
FspBI CTAG 6 cut(s) 195, 218, 581, 810, 1739, 2623
GluI GCNGC 8 cut(s) 576, 669, 890, 921, 1029, 2268, 2420, 2620
GsaI CCCAGC 1 cut(s) 837
GsuI CTGGAG 3 cut(s) 954, 2226, 2267
HaeIII GGCC 9 cut(s) 467, 1073, 1501, 1577, 2106, 2328, 2358, 2364, 2615
HapII CCGG 3 cut(s) 823, 1573, 2080
HgaI GACGC 1 cut(s) 230
HincII GTYRAC 4 cut(s) 550, 1366, 1905, 1933
HindII GTYRAC 4 cut(s) 550, 1366, 1905, 1933
HindIII AAGCTT 3 cut(s) 251, 326, 2558
HinfI GANTC 9 cut(s) 268, 556, 827, 967, 1060, 1172, 1367, 1619, 2175
HpaI GTTAAC 1 cut(s) 1933
HpaII CCGG 3 cut(s) 823, 1573, 2080
HphI GGTGA 6 cut(s) 74, 287, 331, 863, 970, 1138
Hpy166II GTNNAC 8 cut(s) 550, 842, 1366, 1661, 1905, 1933, 2055, 2488
Hpy8I GTNNAC 8 cut(s) 550, 842, 1366, 1661, 1905, 1933, 2055, 2488
Hpy99I CGWCG 2 cut(s) 32, 41
HpyCH4III ACNGT 9 cut(s) 132, 681, 725, 769, 857, 982, 1533, 1570, 2587
HpyCH4IV ACGT 1 cut(s) 1935
HpyF10VI GCNNNNNNNGC 6 cut(s) 575, 926, 1139, 2033, 2042, 2393
HpySE526I ACGT 1 cut(s) 1935
KspAI GTTAAC 1 cut(s) 1933
LmnI GCTCC 5 cut(s) 119, 428, 945, 1125, 1881
Lsp1109I GCAGC 6 cut(s) 587, 680, 907, 1015, 2254, 2431
LweI GCATC 5 cut(s) 221, 1561, 2023, 2032, 2584
MaeI CTAG 6 cut(s) 195, 218, 581, 810, 1739, 2623
MaeII ACGT 1 cut(s) 1935
MaeIII GTNAC 7 cut(s) 319, 415, 745, 851, 949, 1075, 2581
MbiI CCGCTC 2 cut(s) 114, 526
MfeI CAATTG 2 cut(s) 1359, 1371
MflI RGATCY 5 cut(s) 591, 1262, 1513, 2066, 2570
MhlI GDGCHC 8 cut(s) 143, 767, 942, 1300, 1592, 1878, 2330, 2435
MlsI TGGCCA 2 cut(s) 2106, 2615
MluNI TGGCCA 2 cut(s) 2106, 2615
MlyI GAGTC 4 cut(s) 836, 961, 1054, 1361
MmeI TCCRAC 3 cut(s) 470, 2131, 2222
Mox20I TGGCCA 2 cut(s) 2106, 2615
Mph1103I ATGCAT 1 cut(s) 2314
MroXI GAANNNNTTC 1 cut(s) 2158
MscI TGGCCA 2 cut(s) 2106, 2615
MseI TTAA 6 cut(s) 624, 998, 1019, 1743, 1932, 2184
MslI CAYNNNNRTG 3 cut(s) 479, 1094, 1428
Msp20I TGGCCA 2 cut(s) 2106, 2615
MspA1I CMGCKG 2 cut(s) 2245, 2619
MspI CCGG 3 cut(s) 823, 1573, 2080
MspR9I CCNGG 5 cut(s) 824, 1574, 1697, 2103, 2360
MunI CAATTG 2 cut(s) 1359, 1371
Mva1269I GAATGC 2 cut(s) 1567, 2421
MvaI CCWGG 3 cut(s) 1697, 2103, 2360
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 6 cut(s) 575, 926, 1139, 2033, 2042, 2393
NciI CCSGG 2 cut(s) 824, 1574
NcoI CCATGG 3 cut(s) 943, 1611, 2322
NlaIV GGNNCC 7 cut(s) 505, 593, 1515, 1609, 2068, 2328, 2462
NmuCI GTSAC 6 cut(s) 319, 415, 851, 949, 1075, 2581
NruI TCGCGA 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 2314
NspI RCATGY 1 cut(s) 1964
PaeR7I CTCGAG 1 cut(s) 875
PagI TCATGA 1 cut(s) 1302
PaqCI CACCTGC 1 cut(s) 839
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 36
PctI GAATGC 2 cut(s) 1567, 2421
PdmI GAANNNNTTC 1 cut(s) 2158
PfeI GAWTC 5 cut(s) 268, 556, 1172, 1619, 2175
PflMI CCANNNNNTGG 2 cut(s) 1486, 2204
PfoI TCCNGGA 1 cut(s) 822
PkrI GCNGC 8 cut(s) 577, 670, 891, 922, 1030, 2269, 2421, 2621
Ple19I CGATCG 1 cut(s) 34
PleI GAGTC 4 cut(s) 835, 961, 1054, 1361
PpsI GAGTC 4 cut(s) 835, 961, 1054, 1361
PpuMI RGGWCCY 3 cut(s) 2251, 2460, 2506
PsiI TTATAA 1 cut(s) 1542
Psp124BI GAGCTC 2 cut(s) 942, 1300
Psp1406I AACGTT 1 cut(s) 1935
Psp5II RGGWCCY 3 cut(s) 2251, 2460, 2506
Psp6I CCWGG 3 cut(s) 1695, 2101, 2358
PspEI GGTNACC 3 cut(s) 319, 745, 851
PspFI CCCAGC 1 cut(s) 833
PspGI CCWGG 3 cut(s) 1695, 2101, 2358
PspN4I GGNNCC 7 cut(s) 505, 593, 1515, 1609, 2068, 2328, 2462
PspOMI GGGCCC 1 cut(s) 2326
PspPPI RGGWCCY 3 cut(s) 2251, 2460, 2506
PsrI GAACNNNNNNTAC 2 cut(s) 496, 528
PstNI CAGNNNCTG 3 cut(s) 1895, 1919, 2393
PsuI RGATCY 5 cut(s) 591, 1262, 1513, 2066, 2570
PvuI CGATCG 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 2245
RruI TCGCGA 1 cut(s) 36
RsaI GTAC 9 cut(s) 149, 678, 841, 1349, 1535, 1660, 1806, 1976, 2295
RsaNI GTAC 9 cut(s) 148, 677, 840, 1348, 1534, 1659, 1805, 1975, 2294
RseI CAYNNNNRTG 3 cut(s) 479, 1094, 1428
Rsr2I CGGWCCG 1 cut(s) 1033
RsrII CGGWCCG 1 cut(s) 1033
SacI GAGCTC 2 cut(s) 942, 1300
SaqAI TTAA 6 cut(s) 624, 998, 1019, 1743, 1932, 2184
SatI GCNGC 8 cut(s) 576, 669, 890, 921, 1029, 2268, 2420, 2620
ScaI AGTACT 2 cut(s) 149, 2295
SchI GAGTC 4 cut(s) 836, 961, 1054, 1361
ScrFI CCNGG 5 cut(s) 824, 1574, 1697, 2103, 2360
SduI GDGCHC 8 cut(s) 143, 767, 942, 1300, 1592, 1878, 2330, 2435
SfaNI GCATC 5 cut(s) 221, 1561, 2023, 2032, 2584
SfcI CTRYAG 1 cut(s) 300
Sfr274I CTCGAG 1 cut(s) 875
SinI GGWCC 4 cut(s) 1033, 2251, 2460, 2506
SlaI CTCGAG 1 cut(s) 875
SmiMI CAYNNNNRTG 3 cut(s) 479, 1094, 1428
SmlI CTYRAG 5 cut(s) 875, 1556, 1748, 2255, 2455
SmoI CTYRAG 5 cut(s) 875, 1556, 1748, 2255, 2455
SpeI ACTAGT 1 cut(s) 809
SspMI CTAG 6 cut(s) 195, 218, 581, 810, 1739, 2623
SstI GAGCTC 2 cut(s) 942, 1300
StyD4I CCNGG 5 cut(s) 822, 1572, 1695, 2101, 2358
StyI CCWWGG 5 cut(s) 943, 1611, 1809, 2322, 2410
TaaI ACNGT 9 cut(s) 132, 681, 725, 769, 857, 982, 1533, 1570, 2587
TaiI ACGT 1 cut(s) 1938
TaqI TCGA 5 cut(s) 97, 338, 876, 1175, 1546
TatI WGTACW 7 cut(s) 147, 676, 839, 1347, 1658, 1974, 2293
TauI GCSGC 2 cut(s) 892, 2622
TfiI GAWTC 5 cut(s) 268, 556, 1172, 1619, 2175
Tru1I TTAA 6 cut(s) 624, 998, 1019, 1743, 1932, 2184
Tru9I TTAA 6 cut(s) 624, 998, 1019, 1743, 1932, 2184
TscAI CASTG 7 cut(s) 567, 696, 772, 1020, 1573, 1585, 2209
TseFI GTSAC 6 cut(s) 319, 415, 851, 949, 1075, 2581
TseI GCWGC 6 cut(s) 575, 668, 920, 1028, 2267, 2419
Tsp45I GTSAC 6 cut(s) 319, 415, 851, 949, 1075, 2581
TspGWI ACGGA 4 cut(s) 44, 512, 761, 2450
TspRI CASTG 7 cut(s) 567, 696, 772, 1020, 1573, 1585, 2209
Van91I CCANNNNNTGG 2 cut(s) 1486, 2204
VpaK11BI GGWCC 4 cut(s) 1033, 2251, 2460, 2506
XagI CCTNNNNNAGG 2 cut(s) 431, 1046
XapI RAATTY 5 cut(s) 93, 1232, 1451, 1601, 2156
XceI RCATGY 1 cut(s) 1964
XhoI CTCGAG 1 cut(s) 875
XmiI GTMKAC 1 cut(s) 2487
XmnI GAANNNNTTC 1 cut(s) 2158
XspI CTAG 6 cut(s) 195, 218, 581, 810, 1739, 2623
ZrmI AGTACT 2 cut(s) 149, 2295
Zsp2I ATGCAT 1 cut(s) 2314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.