MD05G1080700.v1.1

VID27 cytoplasmic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013857
Physical Location & Seq
Forward (+)
16836805 .. 16840903
4099 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1080700.v1.1.491

Sequence Viewer

Length: 1926 bp
ATGGGAACCGCTCAGAGCCGGGAGGATCACATCACCGACTCCGACGACTACGAGTCAGAGGAGGAGGAGGAACAGAAATCGGAGGACGAAGACCAGTACGACGACGCAGAGGACAACTACCAGCCACCGCAAACCGCTTCTTCGAACCCCAAGTCCAAAACCCTAGAGTCCTCCGTCGATGACGTCGATGCCAGGCTCAAGGCCCTCAAGCTCAAGTACGGCTCTTCTTCCTCCTCTTCCTCCGCCTCCGCCGCTCAAGGCCAGAACGCCGTCAAGCTCTACCTCCATGTCGGTGGCAACACTCCCAAGGCCAAGTGGATCGTCTCCGAGAAGACGGCCTACTCCTTCGTCAAGACCTGTAATGTCGACGGCGACGGTGATTACGACGACGACCGAAGGATGGACCGCGAGGGGGAGTGGGTTCTGAAAGTCGGGTCGAAAGTCCGAGCTAGGGTTTCCACCGACCTGCAATTGAAGATGTTCGGCGATCAGCGCCGTGTCGATTTCGTGTCCAAGGGCGTCTGGGCTCTGAAGTTTTACACCGACGAGCAGTACCGGAGGTTCGTGACGGAGTTTCAGGACTACTTGTTCGAGAACGTGTACGGAGTCAAGGCCACGGAGGAGAACAAGGTCAAGGTCTACGGCAAGGAGTTTCTCGGGTGGGTTAAGCCGGAGGCGGCGGATGATTCGGCCTGGGACTATGAGGATTCCGAGAAAAGCCCAAAGTCTGCCACGCCGGTGCGGCCCAGCCACGACTTGATGGAGGAGTTCGAGGAGGCCGCCAATGGAGGGGTCCAGAGCTTAACTCTCGGCGCATTGGACAATAGCTACTTGGTGAACGAGAATGGTGTTCAGTTTTACCGGAATTTCAACCATGGGATTCATGGGAAGGGAGTGTGTGCGAAATTCGATACCGGAGGTTCAAGTTTGGGGAAATCGACACCCAAGAAGGCTTTGTTAATGAGGGCTGAGACTAATATGCTACTTATGAGTCCATTTAAGGAAGGCAAGCCTCAAGCTAATGGGCTGCAGCAGCTCGATATTGAGACGGGCAAGATTGTCACTGAATGGAAGTTTCAAAAGGATGGAACAGATATTACGATGAGGGATATCACTAATGACACTAAAGGGTCCCAATTGGATCCTTCTGAATCGACCTTTTTGGGTTTGGATGATAACAGGCTTTGTCAGTGGGATATGCGTGACCGTGCTGGAATGGTTCAGAACATTGCTAATGCGAATTCCCCGGTCCTGCATTGGACTCAGGGGCATCAGTTTTCTCGAGGGACCAATTTTCAGTGCTTTGCTACTACTGGAGATGGGTCTATTGTTGTTGGGGGTCTGGAAGGGAAGATAAGGTTGTATTCCAAGACCTCCATGAGGCAGGCAAAGACTGCTTTTCCGGGGCTCGGTTCTCCCATTACGCATATTGATGTAACTTATGATGGGAAGTGGGTGTTAGGTACCACTGATACTTATTTGGTACTTATTTGCACTCTGTTCACTGACAAAGATGGAAAGACCAAGACTGGATTTAGTGGTAGAGCGGGAAACAAGATACCGGCTCCCAGGCTGTTGAAGCTCACCCCTTTGGACTCACATTTGGCTGGCACAGACAATAAATTTCAACGGGGGCATTTCTCATGGGTCACTGAGAACGGTAAACAAGAGCGCCACCTAGTCGCAACAGTTGGAAAATTCAGTGTGGTATGGGATTTCCAGCAGGTGAAGAACAGTGCTCATGATTGTTACAGGAATCAGCAAGGCCTCAAGAGTTGTTACTGTTACAAGATTCTGTTGAAGGATGAATCCATCGTTGAGAGTCGCTTTATGCACGACAATTATGGTACCAACTCCCCCGAAGCTCCACTGGTGGTGGCAACCCCGATGAAAGTGAGCTCAATCAGTCTTTCCGGCAAACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VID27

Protein Analysis

642

Amino Acids

71.51

Weight (kDa)

5.68

Isoelectric Point (pI)

32.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7135 PF23581 48 - 215 8.8e-74 Domain of unknown function (DUF7135)
VID27 PF08553 274 - 585 5.3e-43 VID27 C-terminal WD40-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19240
fragaria_vesca FvH4_2g06100
malus_domestica MD05G1080700.v1.1
prunus_persica Prupe.8G124300_v2.0.a1
pyrus_communis pycom05g07340
rosa_chinensis RchiOBHm_Chr6g0257121
rosa_laevigata RLG00000014677
rosa_multiflora Rmu_co8449469.1_g000001 Rmu_sc0000659.1_g000002
rosa_roxburghii Rroxscaffold_7G00208510
rosa_rugosa Rorug05G0575200
rosa_samantha Rh2BG009300 Rh6AG093100 Rh6BG084000 Rh6CG080400 Rh6DG075800
rosa_wichuraiana Rw6G008010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1714
AatII GACGTC 1 cut(s) 186
Acc36I ACCTGC 2 cut(s) 474, 1714
Acc65I GGTACC 2 cut(s) 1463, 1847
AccB1I GGYRCC 2 cut(s) 1463, 1847
AccBSI CCGCTC 3 cut(s) 11, 254, 1547
AccI GTMKAC 2 cut(s) 366, 639
AccII CGCG 1 cut(s) 408
AclWI GGATC 4 cut(s) 33, 326, 1136, 1149
AcsI RAATTY 5 cut(s) 865, 905, 1240, 1622, 1697
AcuI CTGAAG 1 cut(s) 551
AcyI GRCGYC 2 cut(s) 183, 519
AfaI GTAC 7 cut(s) 98, 218, 554, 602, 1465, 1485, 1849
AfiI CCNNNNNNNGG 6 cut(s) 400, 412, 451, 789, 1380, 1409
AflIII ACRYGT 1 cut(s) 597
AgsI TTSAA 7 cut(s) 475, 871, 924, 1079, 1579, 1628, 1801
AhdI GACNNNNNGTC 1 cut(s) 52
AjnI CCWGG 3 cut(s) 191, 692, 1568
AleI CACNNNNGTG 1 cut(s) 737
AloI GAACNNNNNNTCC 4 cut(s) 137, 169, 572, 604
Alw21I GWGCWC 2 cut(s) 1741, 1901
Alw26I GTCTC 3 cut(s) 328, 965, 1040
AlwI GGATC 4 cut(s) 33, 326, 1136, 1149
Ama87I CYCGRG 2 cut(s) 656, 1281
AoxI GGCC 9 cut(s) 201, 259, 309, 336, 612, 690, 743, 777, 1765
ApeKI GCWGC 3 cut(s) 1027, 1030, 1033
ApoI RAATTY 5 cut(s) 865, 905, 1240, 1622, 1697
ArsI GACNNNNNNTTYG 4 cut(s) 137, 169, 572, 604
Asp700I GAANNNNTTC 1 cut(s) 479
Asp718I GGTACC 2 cut(s) 1463, 1847
AspLEI GCGC 3 cut(s) 495, 815, 1674
AspS9I GGNCC 7 cut(s) 202, 403, 744, 793, 1131, 1249, 1287
AsuC2I CCSGG 3 cut(s) 20, 1247, 1404
AsuHPI GGTGA 5 cut(s) 25, 389, 847, 1576, 1738
AsuII TTCGAA 1 cut(s) 143
AvaI CYCGRG 2 cut(s) 656, 1281
AvaII GGWCC 5 cut(s) 403, 793, 1131, 1249, 1287
BamHI GGATCC 1 cut(s) 1141
BanI GGYRCC 2 cut(s) 1463, 1847
BanII GRGCYC 3 cut(s) 529, 1410, 1901
BarI GAAGNNNNNNTAC 2 cut(s) 323, 355
BbsI GAAGAC 2 cut(s) 96, 338
Bbv12I GWGCWC 2 cut(s) 1741, 1901
BbvI GCAGC 3 cut(s) 1014, 1042, 1045
BccI CCATC 7 cut(s) 394, 754, 1079, 1313, 1439, 1508, 1820
BceAI ACGGC 6 cut(s) 235, 254, 351, 385, 480, 658
BciT130I CCWGG 3 cut(s) 193, 694, 1570
BcnI CCSGG 3 cut(s) 20, 1247, 1404
BcoDI GTCTC 3 cut(s) 328, 965, 1040
BfaI CTAG 3 cut(s) 164, 450, 1679
BfmI CTRYAG 1 cut(s) 1028
BfoI RGCGCY 2 cut(s) 496, 1675
BfuAI ACCTGC 2 cut(s) 474, 1714
BglI GCCNNNNNGGC 1 cut(s) 742
BisI GCNGC 7 cut(s) 252, 678, 743, 780, 1028, 1031, 1034
BlsI GCNGC 7 cut(s) 253, 679, 744, 781, 1029, 1032, 1035
Bme1390I CCNGG 6 cut(s) 20, 193, 694, 1247, 1404, 1570
Bme18I GGWCC 5 cut(s) 403, 793, 1131, 1249, 1287
BmeRI GACNNNNNGTC 1 cut(s) 52
BmeT110I CYCGRG 2 cut(s) 656, 1281
BmgT120I GGNCC 7 cut(s) 202, 403, 744, 793, 1131, 1249, 1287
BmiI GGNNCC 9 cut(s) 7, 794, 1132, 1133, 1143, 1288, 1465, 1566, 1849
BmrFI CCNGG 6 cut(s) 20, 193, 694, 1247, 1404, 1570
BmsI GCATC 2 cut(s) 178, 1279
BpiI GAAGAC 2 cut(s) 96, 338
BplI GAGNNNNNCTC 2 cut(s) 790, 822
BpmI CTGGAG 1 cut(s) 1335
Bpu14I TTCGAA 1 cut(s) 143
BpuEI CTTGAG 6 cut(s) 182, 191, 197, 240, 999, 1754
BpuMI CCSGG 3 cut(s) 20, 1247, 1404
BsaHI GRCGYC 2 cut(s) 183, 519
BsaJI CCNNGG 8 cut(s) 306, 513, 615, 693, 874, 1245, 1403, 1568
BsaWI WCCGGW 3 cut(s) 555, 861, 914
Bsc4I CCNNNNNNNGG 6 cut(s) 400, 412, 451, 789, 1380, 1409
Bse118I RCCGGY 2 cut(s) 736, 1561
Bse1I ACTGG 4 cut(s) 94, 1318, 1534, 1875
Bse3DI GCAATG 1 cut(s) 1227
BseBI CCWGG 3 cut(s) 193, 694, 1570
BseDI CCNNGG 8 cut(s) 306, 513, 615, 693, 874, 1245, 1403, 1568
BseGI GGATG 5 cut(s) 405, 688, 1090, 1177, 1810
BseLI CCNNNNNNNGG 6 cut(s) 400, 412, 451, 789, 1380, 1409
BseMI GCAATG 1 cut(s) 1227
BseMII CTCAG 4 cut(s) 26, 960, 1277, 1644
BseNI ACTGG 4 cut(s) 94, 1318, 1534, 1875
BseRI GAGGAG 7 cut(s) 74, 77, 80, 223, 635, 779, 788
BseXI GCAGC 3 cut(s) 1014, 1042, 1045
BseYI CCCAGC 1 cut(s) 746
Bsh1236I CGCG 1 cut(s) 408
Bsh1285I CGRYCG 1 cut(s) 394
BshFI GGCC 9 cut(s) 203, 261, 311, 338, 614, 692, 745, 779, 1767
BshNI GGYRCC 2 cut(s) 1463, 1847
BsiEI CGRYCG 1 cut(s) 394
BsiHKAI GWGCWC 2 cut(s) 1741, 1901
BsiHKCI CYCGRG 2 cut(s) 656, 1281
BslFI GGGAC 3 cut(s) 710, 1117, 1300
BslI CCNNNNNNNGG 6 cut(s) 400, 412, 451, 789, 1380, 1409
BsmAI GTCTC 3 cut(s) 328, 965, 1040
BsmBI CGTCTC 2 cut(s) 328, 1040
BsmFI GGGAC 3 cut(s) 710, 1117, 1300
BsnI GGCC 9 cut(s) 203, 261, 311, 338, 614, 692, 745, 779, 1767
BsoBI CYCGRG 2 cut(s) 656, 1281
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 4 cut(s) 529, 1410, 1741, 1901
Bsp143I GATC 4 cut(s) 25, 318, 487, 1141
Bsp19I CCATGG 1 cut(s) 874
BspANI GGCC 9 cut(s) 203, 261, 311, 338, 614, 692, 745, 779, 1767
BspCNI CTCAG 4 cut(s) 25, 961, 1276, 1645
BspFNI CGCG 1 cut(s) 408
BspHI TCATGA 1 cut(s) 1741
BspLI GGNNCC 9 cut(s) 7, 794, 1132, 1133, 1143, 1288, 1465, 1566, 1849
BspMAI CTGCAG 1 cut(s) 1032
BspMI ACCTGC 2 cut(s) 474, 1714
BspPI GGATC 4 cut(s) 33, 326, 1136, 1149
BspQI GCTCTTC 1 cut(s) 229
BspT104I TTCGAA 1 cut(s) 143
BspT107I GGYRCC 2 cut(s) 1463, 1847
BsrBI CCGCTC 3 cut(s) 11, 254, 1547
BsrDI GCAATG 1 cut(s) 1227
BsrFI RCCGGY 2 cut(s) 736, 1561
BsrI ACTGG 4 cut(s) 94, 1318, 1534, 1875
BssAI RCCGGY 2 cut(s) 736, 1561
BssECI CCNNGG 8 cut(s) 306, 513, 615, 693, 874, 1245, 1403, 1568
BssMI GATC 4 cut(s) 25, 318, 487, 1141
BssNI GRCGYC 2 cut(s) 183, 519
BssT1I CCWWGG 3 cut(s) 306, 513, 874
Bst2UI CCWGG 3 cut(s) 193, 694, 1570
Bst4CI ACNGT 6 cut(s) 377, 1208, 1661, 1690, 1736, 1784
Bst6I CTCTTC 2 cut(s) 229, 241
BstACI GRCGYC 2 cut(s) 183, 519
BstAPI GCANNNNNTGC 1 cut(s) 1394
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 3 cut(s) 1010, 1386, 1609
BstDEI CTNAG 4 cut(s) 12, 969, 1263, 1653
BstDSI CCRYGG 2 cut(s) 615, 874
BstENI CCTNNNNNAGG 1 cut(s) 1378
BstF5I GGATG 5 cut(s) 405, 688, 1090, 1177, 1810
BstFNI CGCG 1 cut(s) 408
BstH2I RGCGCY 2 cut(s) 496, 1675
BstHHI GCGC 3 cut(s) 495, 815, 1674
BstKTI GATC 4 cut(s) 28, 321, 490, 1144
BstMAI GTCTC 3 cut(s) 328, 965, 1040
BstMBI GATC 4 cut(s) 25, 318, 487, 1141
BstMCI CGRYCG 1 cut(s) 394
BstMWI GCNNNNNNNGC 6 cut(s) 251, 492, 742, 1033, 1394, 1579
BstNI CCWGG 3 cut(s) 193, 694, 1570
BstSCI CCNGG 6 cut(s) 18, 191, 692, 1245, 1402, 1568
BstSFI CTRYAG 1 cut(s) 1028
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 3 cut(s) 1014, 1042, 1045
BstV2I GAAGAC 2 cut(s) 96, 338
BstX2I RGATCY 1 cut(s) 1141
BstXI CCANNNNNNTGG 1 cut(s) 293
BstYI RGATCY 1 cut(s) 1141
BsuRI GGCC 9 cut(s) 203, 261, 311, 338, 614, 692, 745, 779, 1767
BtgI CCRYGG 2 cut(s) 615, 874
BtsCI GGATG 5 cut(s) 405, 688, 1090, 1177, 1810
BtsIMutI CAGTG 9 cut(s) 1062, 1196, 1304, 1467, 1503, 1650, 1708, 1741, 1868
BveI ACCTGC 2 cut(s) 474, 1714
Cac8I GCNNGC 3 cut(s) 1010, 1386, 1609
CciI TCATGA 1 cut(s) 1741
CfoI GCGC 3 cut(s) 495, 815, 1674
Cfr10I RCCGGY 2 cut(s) 736, 1561
Cfr13I GGNCC 7 cut(s) 202, 403, 744, 793, 1131, 1249, 1287
CseI GACGC 2 cut(s) 113, 508
Csp6I GTAC 7 cut(s) 97, 217, 553, 601, 1464, 1484, 1848
CviAII CATG 6 cut(s) 287, 875, 884, 1378, 1644, 1742
CviQI GTAC 7 cut(s) 97, 217, 553, 601, 1464, 1484, 1848
DdeI CTNAG 4 cut(s) 12, 969, 1263, 1653
DpnI GATC 4 cut(s) 27, 320, 489, 1143
DpnII GATC 4 cut(s) 25, 318, 487, 1141
DriI GACNNNNNGTC 1 cut(s) 52
Eam1104I CTCTTC 2 cut(s) 229, 241
Eam1105I GACNNNNNGTC 1 cut(s) 52
EarI CTCTTC 2 cut(s) 229, 241
EciI GGCGGA 3 cut(s) 232, 238, 695
Ecl136II GAGCTC 1 cut(s) 1899
Eco130I CCWWGG 3 cut(s) 306, 513, 874
Eco147I AGGCCT 1 cut(s) 1767
Eco24I GRGCYC 3 cut(s) 529, 1410, 1901
Eco32I GATATC 1 cut(s) 1111
Eco47I GGWCC 5 cut(s) 403, 793, 1131, 1249, 1287
Eco53kI GAGCTC 1 cut(s) 1899
Eco57I CTGAAG 1 cut(s) 551
Eco88I CYCGRG 2 cut(s) 656, 1281
EcoICRI GAGCTC 1 cut(s) 1899
EcoNI CCTNNNNNAGG 1 cut(s) 1378
EcoO109I RGGNCCY 2 cut(s) 202, 1131
EcoRI GAATTC 1 cut(s) 1240
EcoRII CCWGG 3 cut(s) 191, 692, 1568
EcoRV GATATC 1 cut(s) 1111
EcoT14I CCWWGG 3 cut(s) 306, 513, 874
EcoT38I GRGCYC 3 cut(s) 529, 1410, 1901
ErhI CCWWGG 3 cut(s) 306, 513, 874
Esp3I CGTCTC 2 cut(s) 328, 1040
FaeI CATG 6 cut(s) 290, 878, 887, 1381, 1647, 1745
FaqI GGGAC 3 cut(s) 710, 1117, 1300
FatI CATG 6 cut(s) 286, 874, 883, 1377, 1643, 1741
FauI CCCGC 1 cut(s) 1540
FblI GTMKAC 2 cut(s) 366, 639
Fnu4HI GCNGC 7 cut(s) 252, 678, 743, 780, 1028, 1031, 1034
FokI GGATG 5 cut(s) 412, 695, 1097, 1184, 1817
FriOI GRGCYC 3 cut(s) 529, 1410, 1901
Fsp4HI GCNGC 7 cut(s) 252, 678, 743, 780, 1028, 1031, 1034
FspBI CTAG 3 cut(s) 164, 450, 1679
GlaI GCGC 3 cut(s) 494, 814, 1673
GluI GCNGC 7 cut(s) 252, 678, 743, 780, 1028, 1031, 1034
GsaI CCCAGC 1 cut(s) 750
GsuI CTGGAG 1 cut(s) 1335
HaeII RGCGCY 2 cut(s) 496, 1675
HaeIII GGCC 9 cut(s) 203, 261, 311, 338, 614, 692, 745, 779, 1767
HgaI GACGC 2 cut(s) 113, 508
HhaI GCGC 3 cut(s) 495, 815, 1674
Hin1I GRCGYC 2 cut(s) 183, 519
Hin1II CATG 6 cut(s) 290, 878, 887, 1381, 1647, 1745
Hin6I GCGC 3 cut(s) 493, 813, 1672
HinP1I GCGC 3 cut(s) 493, 813, 1672
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HphI GGTGA 5 cut(s) 25, 389, 847, 1576, 1738
Hpy166II GTNNAC 6 cut(s) 367, 601, 640, 838, 1503, 1664
Hpy188III TCNNGA 9 cut(s) 352, 565, 578, 592, 796, 1281, 1343, 1742, 1771
Hpy8I GTNNAC 6 cut(s) 367, 601, 640, 838, 1503, 1664
HpyAV CCTTC 8 cut(s) 355, 390, 883, 943, 998, 1155, 1340, 1795
HpyCH4III ACNGT 6 cut(s) 377, 1208, 1661, 1690, 1736, 1784
HpyCH4IV ACGT 2 cut(s) 183, 597
HpyCH4V TGCA 5 cut(s) 469, 1030, 1255, 1494, 1834
HpyF10VI GCNNNNNNNGC 6 cut(s) 251, 492, 742, 1033, 1394, 1579
HpyF3I CTNAG 4 cut(s) 12, 969, 1263, 1653
HpySE526I ACGT 2 cut(s) 183, 597
Hsp92I GRCGYC 2 cut(s) 183, 519
Hsp92II CATG 6 cut(s) 290, 878, 887, 1381, 1647, 1745
HspAI GCGC 3 cut(s) 493, 813, 1672
KflI GGGWCCC 1 cut(s) 1131
KpnI GGTACC 2 cut(s) 1467, 1851
Kzo9I GATC 4 cut(s) 25, 318, 487, 1141
LguI GCTCTTC 1 cut(s) 229
LmnI GCTCC 2 cut(s) 1570, 1870
Lsp1109I GCAGC 3 cut(s) 1014, 1042, 1045
LweI GCATC 2 cut(s) 178, 1279
MaeI CTAG 3 cut(s) 164, 450, 1679
MaeII ACGT 2 cut(s) 183, 597
MaeIII GTNAC 8 cut(s) 565, 1060, 1202, 1435, 1648, 1748, 1778, 1784
MalI GATC 4 cut(s) 27, 320, 489, 1143
MbiI CCGCTC 3 cut(s) 11, 254, 1547
MboI GATC 4 cut(s) 25, 318, 487, 1141
MboII GAAGA 9 cut(s) 101, 132, 216, 219, 228, 343, 487, 1363, 1741
MfeI CAATTG 2 cut(s) 470, 1136
MflI RGATCY 1 cut(s) 1141
MhlI GDGCHC 4 cut(s) 529, 1410, 1741, 1901
MluCI AATT 9 cut(s) 470, 865, 905, 1136, 1240, 1291, 1622, 1697, 1840
MlyI GAGTC 8 cut(s) 32, 62, 176, 615, 1000, 1255, 1589, 1831
MmeI TCCRAC 2 cut(s) 66, 1672
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 4 cut(s) 666, 803, 959, 999
MslI CAYNNNNRTG 3 cut(s) 291, 737, 1431
MspR9I CCNGG 6 cut(s) 20, 193, 694, 1247, 1404, 1570
MunI CAATTG 2 cut(s) 470, 1136
MvaI CCWGG 3 cut(s) 193, 694, 1570
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 6 cut(s) 251, 492, 742, 1033, 1394, 1579
NciI CCSGG 3 cut(s) 20, 1247, 1404
NcoI CCATGG 1 cut(s) 874
NdeII GATC 4 cut(s) 25, 318, 487, 1141
NlaIII CATG 6 cut(s) 290, 878, 887, 1381, 1647, 1745
NlaIV GGNNCC 9 cut(s) 7, 794, 1132, 1133, 1143, 1288, 1465, 1566, 1849
NmeAIII GCCGAG 1 cut(s) 789
NmuCI GTSAC 4 cut(s) 565, 1060, 1202, 1648
NspV TTCGAA 1 cut(s) 143
OliI CACNNNNGTG 1 cut(s) 737
PaeR7I CTCGAG 1 cut(s) 1281
PagI TCATGA 1 cut(s) 1741
PaqCI CACCTGC 1 cut(s) 1714
PceI AGGCCT 1 cut(s) 1767
PciSI GCTCTTC 1 cut(s) 229
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 381
PdmI GAANNNNTTC 1 cut(s) 479
PfeI GAWTC 7 cut(s) 686, 707, 880, 1151, 1756, 1792, 1808
PkrI GCNGC 7 cut(s) 253, 679, 744, 781, 1029, 1032, 1035
PleI GAGTC 8 cut(s) 32, 61, 175, 614, 999, 1255, 1589, 1830
PpsI GAGTC 8 cut(s) 32, 61, 175, 614, 999, 1255, 1589, 1830
PpuMI RGGWCCY 1 cut(s) 1131
Psp124BI GAGCTC 1 cut(s) 1901
Psp5II RGGWCCY 1 cut(s) 1131
Psp6I CCWGG 3 cut(s) 191, 692, 1568
PspFI CCCAGC 1 cut(s) 746
PspGI CCWGG 3 cut(s) 191, 692, 1568
PspN4I GGNNCC 9 cut(s) 7, 794, 1132, 1133, 1143, 1288, 1465, 1566, 1849
PspPI GGNCC 7 cut(s) 202, 403, 744, 793, 1131, 1249, 1287
PspPPI RGGWCCY 1 cut(s) 1131
PsrI GAACNNNNNNTAC 4 cut(s) 545, 577, 1081, 1113
PstI CTGCAG 1 cut(s) 1032
PsuI RGATCY 1 cut(s) 1141
RsaI GTAC 7 cut(s) 98, 218, 554, 602, 1465, 1485, 1849
RsaNI GTAC 7 cut(s) 97, 217, 553, 601, 1464, 1484, 1848
RseI CAYNNNNRTG 3 cut(s) 291, 737, 1431
SacI GAGCTC 1 cut(s) 1901
SalI GTCGAC 1 cut(s) 365
SapI GCTCTTC 1 cut(s) 229
SaqAI TTAA 4 cut(s) 666, 803, 959, 999
SatI GCNGC 7 cut(s) 252, 678, 743, 780, 1028, 1031, 1034
Sau3AI GATC 4 cut(s) 25, 318, 487, 1141
Sau96I GGNCC 7 cut(s) 202, 403, 744, 793, 1131, 1249, 1287
SchI GAGTC 8 cut(s) 32, 62, 176, 615, 1000, 1255, 1589, 1831
ScrFI CCNGG 6 cut(s) 20, 193, 694, 1247, 1404, 1570
SduI GDGCHC 4 cut(s) 529, 1410, 1741, 1901
SfaNI GCATC 2 cut(s) 178, 1279
SfcI CTRYAG 1 cut(s) 1028
Sfr274I CTCGAG 1 cut(s) 1281
SfuI TTCGAA 1 cut(s) 143
SgrAI CRCCGGYG 1 cut(s) 736
SinI GGWCC 5 cut(s) 403, 793, 1131, 1249, 1287
SlaI CTCGAG 1 cut(s) 1281
SmiMI CAYNNNNRTG 3 cut(s) 291, 737, 1431
SmlI CTYRAG 7 cut(s) 197, 206, 212, 255, 1014, 1281, 1769
SmoI CTYRAG 7 cut(s) 197, 206, 212, 255, 1014, 1281, 1769
Sse9I AATT 9 cut(s) 470, 865, 905, 1136, 1240, 1291, 1622, 1697, 1840
SseBI AGGCCT 1 cut(s) 1767
SspMI CTAG 3 cut(s) 164, 450, 1679
SstI GAGCTC 1 cut(s) 1901
StuI AGGCCT 1 cut(s) 1767
StyD4I CCNGG 6 cut(s) 18, 191, 692, 1245, 1402, 1568
StyI CCWWGG 3 cut(s) 306, 513, 874
TaaI ACNGT 6 cut(s) 377, 1208, 1661, 1690, 1736, 1784
TaiI ACGT 2 cut(s) 186, 600
TaqII GACCGA 1 cut(s) 408
TasI AATT 9 cut(s) 470, 865, 905, 1136, 1240, 1291, 1622, 1697, 1840
TauI GCSGC 4 cut(s) 254, 680, 745, 782
TfiI GAWTC 7 cut(s) 686, 707, 880, 1151, 1756, 1792, 1808
Tru1I TTAA 4 cut(s) 666, 803, 959, 999
Tru9I TTAA 4 cut(s) 666, 803, 959, 999
TscAI CASTG 9 cut(s) 1069, 1196, 1304, 1474, 1510, 1657, 1708, 1741, 1875
TseFI GTSAC 4 cut(s) 565, 1060, 1202, 1648
TseI GCWGC 3 cut(s) 1027, 1030, 1033
Tsp45I GTSAC 4 cut(s) 565, 1060, 1202, 1648
TspDTI ATGAA 3 cut(s) 872, 1821, 1904
TspGWI ACGGA 4 cut(s) 163, 584, 618, 632
TspRI CASTG 9 cut(s) 1069, 1196, 1304, 1474, 1510, 1657, 1708, 1741, 1875
VpaK11BI GGWCC 5 cut(s) 403, 793, 1131, 1249, 1287
XagI CCTNNNNNAGG 1 cut(s) 1378
XapI RAATTY 5 cut(s) 865, 905, 1240, 1622, 1697
XcmI CCANNNNNNNNNTGG 1 cut(s) 881
XhoI CTCGAG 1 cut(s) 1281
XmiI GTMKAC 2 cut(s) 366, 639
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 3 cut(s) 164, 450, 1679
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.