Rh6DG075800

VID27 cytoplasmic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013857
Physical Location & Seq
Reverse (-)
8664503 .. 8667710
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG075800.1

Sequence Viewer

Length: 1923 bp
ATGGGAACCTCCCAGAGCCGCGAGGACTACATCTCCGACTCCGACGACTACAAAGAGGAGGAGGAGGAGGAGGAAGAAGAAGAACGATCCGACGATACAGAAGAGTACCAAGACGCTCGAGAGAAACAGCCCACCCCAATCGGCGATCTCGACGCCAAGCTTCAAGCCTTGAAGCTCAAGTACGGAACTCCTTCCTCTTCCTCCTCCACCCCCTCGTCCGCCGTCTCACATAACGCCGTCAAGCTCTACCTCCACATCGGCGGCAACGCTCCCAACGCCAAGTGGATCGTCTCCAACAAGCTCACCTCCTTCTCCTTCGTCAAGACCTCCAACGTCGACGGCGACAGCGATGACGACGACGACGACGACGACTACGAGGGATCCTCGTCGGATGGTAAAGGACAGTGGATTCTCAAGGTTGGGGGCAAGGTCCGAGCTAGGGTTTCCACCGACATGCAATTGAAGATGTTTGGCGATCAGCGACGTGTGGATTTCATGTCTAGAGGCGTCTGGGCTCTGAAATTCTTCACCGACGAGCAGTACCGGAGATTCGTCACCGAATTTCAGGACTGCTTGTTTGAGAACGTGTACGGGCTTAAGGCGACGGAGGAGAACAAGGTCAAGATTTACGGTAAGGAGTTTTTGGGGTGGGTGAAGCCGGAGGCGGCGGATGACTCGGCGTGGGAGTATGCTGACGATTATGACATGGCGGCCGGGAGCCCCAATTCGGTGACGCCGGTCCGCTCCAACCAGCAATTGATGGAGGAGTTTGAGGAGGCTGCGAATGGAGGTGTTCAGAGCTTGACGCTTGGTGCATTGGATAATAGTTTCTTGGTGAATGATTCTGGTGTTCAGGTGTATAGGAATTTCAGAGATGGGATTCATGGGAAGGGTGTGGTTGCGAAATTCGACAGTGGGGGTGGTTCGAAATCGACACCGAAGAAGGCTCTGCTTATGAGGGGGGAGACTAATATGATGCTTATGAGTCCACTGAAGGAGGGAAAGCCTCAGGCAAATGGGCTTCAGCAGCTGGACATTGAGACTGGGAAGATTGTCACGGAATGGAAGTTTGAGAAGGATGGGACAGAGATTACAATGAGGGATATCACCAATGACACTAAGGAGTCGCAATTGGATCCTTCTGAGTCGACATTTCTGGGTTTGGATGATAACAGGCTTTGCCAGTGGGATATGCGTGAACGCAGAGGAATGGTTCAGAACATTGCTAGTTTGAATTCGCCAGTGCTGCAGTGGTCTCAGGGGCATCAGTTTTCTCGAGGGACGAATTTTCAGTGCTTTGCTACTACTGGAGATGGGTCTATTGTTGTTGGGTCTCTGGATGGGAAGATACGGTTGTATTCCAAGACCTCTATGAGGCAGGCAAAGACTGCTTTCCCAGGGCTGGGTTCCCCCATTACTCATGTTGATGTGACTTACGATGGGAAGTGGGTGTTGGGTACAACTGACACTTACTTGGTCCTTATATGCACTCTCTTCACTGACAAAGATGGAAAGACCAAGACTGGGTTTAGTGGTAGAATGGGAAACAAGATACCAGCTCCCAGGTTGTTGAAGCTGATCCCTCTTGACTCACATTTGGCTGGTGCAGACAATAAGTTTCATGGAGGCCACTTCTCATGGGTCACTGAAAGTGGTAAACAAGAGCGTCACCTTGTTGCAACAGTGGGAAAGTTCAGTGTGATATGGGATTTCCAGCAGGTGAAGAATAGTGCCCATGAATGTTACAGGAATCAGCAAGGCCTCAAGAGTTGTTACTGCTACAAGATCCTTTTGAAGGACGAGTCCATTGTGGAGAGCCGTTTCATGCACGATAAGTATGCTGTCAGCGACTCCCCTGAAGCTCCTCTGGTGGTGGCAACCCCGATGAAAGTCAGCTCGATCAGTCTCTCTGGCAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VID27

Protein Analysis

640

Amino Acids

71.3

Weight (kDa)

5.18

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7135 PF23581 39 - 211 2.8e-72 Domain of unknown function (DUF7135)
VID27 PF08553 268 - 583 1e-45 VID27 C-terminal WD40-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19240
fragaria_vesca FvH4_2g06100
malus_domestica MD05G1080700.v1.1
prunus_persica Prupe.8G124300_v2.0.a1
pyrus_communis pycom05g07340
rosa_chinensis RchiOBHm_Chr6g0257121
rosa_laevigata RLG00000014677
rosa_multiflora Rmu_co8449469.1_g000001 Rmu_sc0000659.1_g000002
rosa_roxburghii Rroxscaffold_7G00208510
rosa_rugosa Rorug05G0575200
rosa_samantha Rh2BG009300 Rh6AG093100 Rh6BG084000 Rh6CG080400 Rh6DG075800
rosa_wichuraiana Rw6G008010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1708
Acc36I ACCTGC 1 cut(s) 1708
AccBSI CCGCTC 1 cut(s) 744
AccI GTMKAC 2 cut(s) 336, 1148
AccII CGCG 1 cut(s) 21
AciI CCGC 7 cut(s) 19, 219, 261, 665, 668, 710, 742
AclWI GGATC 8 cut(s) 81, 293, 375, 388, 1130, 1143, 1573, 1780
AcoI YGGCCR 1 cut(s) 711
AcsI RAATTY 6 cut(s) 521, 560, 865, 905, 1234, 1285
AcuI CTGAAG 3 cut(s) 1007, 1013, 1878
AcyI GRCGYC 3 cut(s) 153, 507, 734
AfaI GTAC 5 cut(s) 107, 182, 542, 590, 1459
AfiI CCNNNNNNNGG 7 cut(s) 439, 727, 1374, 1402, 1403, 1524, 1795
AflII CTTAAG 1 cut(s) 596
AflIII ACRYGT 2 cut(s) 484, 585
AgsI TTSAA 6 cut(s) 164, 172, 463, 1234, 1573, 1795
AjiI CACGTC 1 cut(s) 485
AjnI CCWGG 2 cut(s) 1396, 1562
AjuI GAANNNNNNNTTGG 2 cut(s) 1436, 1468
Alw26I GTCTC 7 cut(s) 229, 295, 959, 1034, 1260, 1338, 1910
AlwI GGATC 8 cut(s) 81, 293, 375, 388, 1130, 1143, 1573, 1780
AlwNI CAGNNNCTG 1 cut(s) 1030
Ama87I CYCGRG 2 cut(s) 117, 1275
AoxI GGCC 3 cut(s) 711, 1627, 1759
ApeKI GCWGC 3 cut(s) 779, 1027, 1246
ApoI RAATTY 6 cut(s) 521, 560, 865, 905, 1234, 1285
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
Asp700I GAANNNNTTC 2 cut(s) 190, 524
AspS9I GGNCC 3 cut(s) 430, 739, 1477
AsuC2I CCSGG 1 cut(s) 715
AsuHPI GGTGA 9 cut(s) 295, 520, 547, 664, 742, 847, 1099, 1661, 1732
AsuII TTCGAA 1 cut(s) 926
AvaI CYCGRG 2 cut(s) 117, 1275
AvaII GGWCC 3 cut(s) 430, 739, 1477
AxyI CCTNAGG 1 cut(s) 1008
BaeGI GKGCMC 1 cut(s) 1735
BaeI ACNNNNGTAYC 2 cut(s) 1340, 1373
BamHI GGATCC 2 cut(s) 380, 1135
BanII GRGCYC 2 cut(s) 517, 722
BarI GAAGNNNNNNTAC 2 cut(s) 164, 196
BbvI GCAGC 3 cut(s) 766, 1039, 1233
BccI CCATC 8 cut(s) 386, 754, 869, 1073, 1307, 1334, 1433, 1502
BceAI ACGGC 4 cut(s) 206, 221, 355, 1803
BcgI CGANNNNNNTGC 2 cut(s) 1820, 1854
BciT130I CCWGG 2 cut(s) 1398, 1564
BcnI CCSGG 1 cut(s) 715
BcoDI GTCTC 7 cut(s) 229, 295, 959, 1034, 1260, 1338, 1910
BfaI CTAG 3 cut(s) 438, 501, 1227
BfmI CTRYAG 1 cut(s) 1247
BfrI CTTAAG 1 cut(s) 596
BfuAI ACCTGC 1 cut(s) 1708
BisI GCNGC 7 cut(s) 19, 262, 666, 711, 780, 1028, 1247
BlsI GCNGC 7 cut(s) 20, 263, 667, 712, 781, 1029, 1248
Bme1390I CCNGG 3 cut(s) 715, 1398, 1564
Bme18I GGWCC 3 cut(s) 430, 739, 1477
BmeT110I CYCGRG 2 cut(s) 117, 1275
BmgBI CACGTC 1 cut(s) 485
BmgT120I GGNCC 3 cut(s) 430, 739, 1477
BmiI GGNNCC 5 cut(s) 7, 382, 719, 1137, 1408
BmrFI CCNGG 3 cut(s) 715, 1398, 1564
BmrI ACTGGG 2 cut(s) 1053, 1533
BmsI GCATC 2 cut(s) 966, 1273
BmuI ACTGGG 2 cut(s) 1053, 1533
BoxI GACNNNNGTC 1 cut(s) 737
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BpmI CTGGAG 1 cut(s) 1329
Bpu14I TTCGAA 1 cut(s) 926
BpuEI CTTGAG 3 cut(s) 161, 398, 1748
BpuMI CCSGG 1 cut(s) 715
BsaHI GRCGYC 3 cut(s) 153, 507, 734
BsaI GGTCTC 2 cut(s) 1260, 1338
BsaJI CCNNGG 3 cut(s) 1396, 1397, 1562
BsaWI WCCGGW 1 cut(s) 543
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 7 cut(s) 439, 727, 1374, 1402, 1403, 1524, 1795
Bse118I RCCGGY 1 cut(s) 736
Bse1I ACTGG 5 cut(s) 1048, 1183, 1241, 1312, 1528
Bse21I CCTNAGG 1 cut(s) 1008
Bse3DI GCAATG 1 cut(s) 1221
BseBI CCWGG 2 cut(s) 1398, 1564
BseDI CCNNGG 3 cut(s) 1396, 1397, 1562
BseGI GGATG 5 cut(s) 397, 676, 1084, 1171, 1345
BseLI CCNNNNNNNGG 7 cut(s) 439, 727, 1374, 1402, 1403, 1524, 1795
BseMI GCAATG 1 cut(s) 1221
BseMII CTCAG 3 cut(s) 1022, 1134, 1271
BseNI ACTGG 5 cut(s) 1048, 1183, 1241, 1312, 1528
BseSI GKGCMC 1 cut(s) 1735
BseX3I CGGCCG 1 cut(s) 711
BseXI GCAGC 3 cut(s) 766, 1039, 1233
BseYI CCCAGC 1 cut(s) 1402
BsgI GTGCAG 1 cut(s) 1626
Bsh1236I CGCG 1 cut(s) 21
Bsh1285I CGRYCG 1 cut(s) 714
BshFI GGCC 3 cut(s) 713, 1629, 1761
BsiEI CGRYCG 1 cut(s) 714
BsiHKCI CYCGRG 2 cut(s) 117, 1275
BsiSI CCGG 4 cut(s) 544, 659, 714, 737
BslFI GGGAC 2 cut(s) 1096, 1294
BslI CCNNNNNNNGG 7 cut(s) 439, 727, 1374, 1402, 1403, 1524, 1795
BsmAI GTCTC 7 cut(s) 229, 295, 959, 1034, 1260, 1338, 1910
BsmBI CGTCTC 2 cut(s) 229, 295
BsmFI GGGAC 2 cut(s) 1096, 1294
BsnI GGCC 3 cut(s) 713, 1629, 1761
Bso31I GGTCTC 2 cut(s) 1260, 1338
BsoBI CYCGRG 2 cut(s) 117, 1275
Bsp119I TTCGAA 1 cut(s) 926
Bsp1286I GDGCHC 3 cut(s) 517, 722, 1735
Bsp143I GATC 9 cut(s) 86, 145, 285, 380, 475, 1135, 1578, 1785, 1899
BspACI CCGC 7 cut(s) 19, 219, 261, 665, 668, 710, 742
BspANI GGCC 3 cut(s) 713, 1629, 1761
BspCNI CTCAG 3 cut(s) 1021, 1135, 1270
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 5 cut(s) 7, 382, 719, 1137, 1408
BspMAI CTGCAG 1 cut(s) 1251
BspMI ACCTGC 1 cut(s) 1708
BspPI GGATC 8 cut(s) 81, 293, 375, 388, 1130, 1143, 1573, 1780
BspT104I TTCGAA 1 cut(s) 926
BspTI CTTAAG 1 cut(s) 596
BspTNI GGTCTC 2 cut(s) 1260, 1338
BsrBI CCGCTC 1 cut(s) 744
BsrDI GCAATG 1 cut(s) 1221
BsrFI RCCGGY 1 cut(s) 736
BsrI ACTGG 5 cut(s) 1048, 1183, 1241, 1312, 1528
BssAI RCCGGY 1 cut(s) 736
BssECI CCNNGG 3 cut(s) 1396, 1397, 1562
BssMI GATC 9 cut(s) 86, 145, 285, 380, 475, 1135, 1578, 1785, 1899
BssNI GRCGYC 3 cut(s) 153, 507, 734
Bst2UI CCWGG 2 cut(s) 1398, 1564
Bst4CI ACNGT 5 cut(s) 405, 632, 914, 1353, 1684
Bst6I CTCTTC 3 cut(s) 96, 202, 1499
BstACI GRCGYC 3 cut(s) 153, 507, 734
BstAFI CTTAAG 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 1388
BstBI TTCGAA 1 cut(s) 926
BstC8I GCNNGC 1 cut(s) 1380
BstDEI CTNAG 4 cut(s) 1008, 1119, 1143, 1257
BstENI CCTNNNNNAGG 2 cut(s) 1372, 1793
BstF5I GGATG 5 cut(s) 397, 676, 1084, 1171, 1345
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 9 cut(s) 89, 148, 288, 383, 478, 1138, 1581, 1788, 1902
BstMAI GTCTC 7 cut(s) 229, 295, 959, 1034, 1260, 1338, 1910
BstMBI GATC 9 cut(s) 86, 145, 285, 380, 475, 1135, 1578, 1785, 1899
BstMCI CGRYCG 1 cut(s) 714
BstMWI GCNNNNNNNGC 4 cut(s) 275, 1027, 1246, 1388
BstNI CCWGG 2 cut(s) 1398, 1564
BstNSI RCATGY 1 cut(s) 457
BstPAI GACNNNNGTC 1 cut(s) 737
BstSCI CCNGG 3 cut(s) 713, 1396, 1562
BstSFI CTRYAG 1 cut(s) 1247
BstSLI GKGCMC 1 cut(s) 1735
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 3 cut(s) 766, 1039, 1233
BstX2I RGATCY 3 cut(s) 380, 1135, 1785
BstYI RGATCY 3 cut(s) 380, 1135, 1785
BstZI CGGCCG 1 cut(s) 711
Bsu36I CCTNAGG 1 cut(s) 1008
BsuRI GGCC 3 cut(s) 713, 1629, 1761
BtgZI GCGATG 1 cut(s) 363
BtrI CACGTC 1 cut(s) 485
BtsCI GGATG 5 cut(s) 397, 676, 1084, 1171, 1345
BtsI GCAGTG 1 cut(s) 1256
BveI ACCTGC 1 cut(s) 1708
Cac8I GCNNGC 1 cut(s) 1380
CaiI CAGNNNCTG 1 cut(s) 1030
Cfr10I RCCGGY 1 cut(s) 736
Cfr13I GGNCC 3 cut(s) 430, 739, 1477
CpoI CGGWCCG 1 cut(s) 739
CseI GACGC 6 cut(s) 122, 161, 496, 742, 814, 1655
Csp6I GTAC 5 cut(s) 106, 181, 541, 589, 1458
CspI CGGWCCG 1 cut(s) 739
CviAII CATG 9 cut(s) 454, 496, 706, 884, 1421, 1622, 1638, 1736, 1825
CviQI GTAC 5 cut(s) 106, 181, 541, 589, 1458
DdeI CTNAG 4 cut(s) 1008, 1119, 1143, 1257
DpnI GATC 9 cut(s) 88, 147, 287, 382, 477, 1137, 1580, 1787, 1901
DpnII GATC 9 cut(s) 86, 145, 285, 380, 475, 1135, 1578, 1785, 1899
EaeI YGGCCR 1 cut(s) 711
EagI CGGCCG 1 cut(s) 711
Eam1104I CTCTTC 3 cut(s) 96, 202, 1499
EarI CTCTTC 3 cut(s) 96, 202, 1499
EciI GGCGGA 2 cut(s) 208, 683
EclXI CGGCCG 1 cut(s) 711
Eco147I AGGCCT 1 cut(s) 1761
Eco24I GRGCYC 2 cut(s) 517, 722
Eco31I GGTCTC 2 cut(s) 1260, 1338
Eco32I GATATC 1 cut(s) 1105
Eco47I GGWCC 3 cut(s) 430, 739, 1477
Eco52I CGGCCG 1 cut(s) 711
Eco57I CTGAAG 3 cut(s) 1007, 1013, 1878
Eco81I CCTNAGG 1 cut(s) 1008
Eco88I CYCGRG 2 cut(s) 117, 1275
EcoNI CCTNNNNNAGG 2 cut(s) 1372, 1793
EcoRI GAATTC 1 cut(s) 1234
EcoRII CCWGG 2 cut(s) 1396, 1562
EcoRV GATATC 1 cut(s) 1105
EcoT38I GRGCYC 2 cut(s) 517, 722
Esp3I CGTCTC 2 cut(s) 229, 295
FaeI CATG 9 cut(s) 457, 499, 709, 887, 1424, 1625, 1641, 1739, 1828
FaqI GGGAC 2 cut(s) 1096, 1294
FatI CATG 9 cut(s) 453, 495, 705, 883, 1420, 1621, 1637, 1735, 1824
FblI GTMKAC 2 cut(s) 336, 1148
Fnu4HI GCNGC 7 cut(s) 19, 262, 666, 711, 780, 1028, 1247
FokI GGATG 5 cut(s) 404, 683, 1091, 1178, 1352
FriOI GRGCYC 2 cut(s) 517, 722
Fsp4HI GCNGC 7 cut(s) 19, 262, 666, 711, 780, 1028, 1247
FspBI CTAG 3 cut(s) 438, 501, 1227
GluI GCNGC 7 cut(s) 19, 262, 666, 711, 780, 1028, 1247
GsaI CCCAGC 1 cut(s) 1406
GsuI CTGGAG 1 cut(s) 1329
HaeIII GGCC 3 cut(s) 713, 1629, 1761
HapII CCGG 4 cut(s) 544, 659, 714, 737
HgaI GACGC 6 cut(s) 122, 161, 496, 742, 814, 1655
Hin1I GRCGYC 3 cut(s) 153, 507, 734
Hin1II CATG 9 cut(s) 457, 499, 709, 887, 1424, 1625, 1641, 1739, 1828
HincII GTYRAC 2 cut(s) 337, 1149
HindII GTYRAC 2 cut(s) 337, 1149
HindIII AAGCTT 1 cut(s) 158
HpaII CCGG 4 cut(s) 544, 659, 714, 737
HphI GGTGA 9 cut(s) 295, 520, 547, 664, 742, 847, 1099, 1661, 1732
Hpy166II GTNNAC 6 cut(s) 337, 589, 989, 1149, 1199, 1658
Hpy8I GTNNAC 6 cut(s) 337, 589, 989, 1149, 1199, 1658
HpyAV CCTTC 9 cut(s) 201, 319, 325, 883, 937, 988, 1069, 1149, 1789
HpyCH4III ACNGT 5 cut(s) 405, 632, 914, 1353, 1684
HpyCH4IV ACGT 3 cut(s) 333, 484, 585
HpyCH4V TGCA 7 cut(s) 457, 815, 1249, 1488, 1607, 1679, 1828
HpyF10VI GCNNNNNNNGC 4 cut(s) 275, 1027, 1246, 1388
HpyF3I CTNAG 4 cut(s) 1008, 1119, 1143, 1257
HpySE526I ACGT 3 cut(s) 333, 484, 585
Hsp92I GRCGYC 3 cut(s) 153, 507, 734
Hsp92II CATG 9 cut(s) 457, 499, 709, 887, 1424, 1625, 1641, 1739, 1828
Kzo9I GATC 9 cut(s) 86, 145, 285, 380, 475, 1135, 1578, 1785, 1899
LmnI GCTCC 5 cut(s) 274, 717, 749, 1564, 1867
Lsp1109I GCAGC 3 cut(s) 766, 1039, 1233
LweI GCATC 2 cut(s) 966, 1273
MaeI CTAG 3 cut(s) 438, 501, 1227
MaeII ACGT 3 cut(s) 333, 484, 585
MaeIII GTNAC 8 cut(s) 553, 730, 1054, 1429, 1642, 1667, 1742, 1772
MalI GATC 9 cut(s) 88, 147, 287, 382, 477, 1137, 1580, 1787, 1901
MbiI CCGCTC 1 cut(s) 744
MboI GATC 9 cut(s) 86, 145, 285, 380, 475, 1135, 1578, 1785, 1899
MfeI CAATTG 3 cut(s) 458, 755, 1130
MflI RGATCY 3 cut(s) 380, 1135, 1785
MhlI GDGCHC 3 cut(s) 517, 722, 1735
MlyI GAGTC 8 cut(s) 32, 668, 994, 1133, 1154, 1583, 1811, 1844
MmeI TCCRAC 7 cut(s) 60, 66, 114, 318, 354, 369, 771
MroXI GAANNNNTTC 2 cut(s) 190, 524
MseI TTAA 1 cut(s) 597
MslI CAYNNNNRTG 2 cut(s) 452, 1425
MspA1I CMGCKG 1 cut(s) 1030
MspCI CTTAAG 1 cut(s) 596
MspI CCGG 4 cut(s) 544, 659, 714, 737
MspR9I CCNGG 3 cut(s) 715, 1398, 1564
MunI CAATTG 3 cut(s) 458, 755, 1130
MvaI CCWGG 2 cut(s) 1398, 1564
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 4 cut(s) 275, 1027, 1246, 1388
NciI CCSGG 1 cut(s) 715
NdeII GATC 9 cut(s) 86, 145, 285, 380, 475, 1135, 1578, 1785, 1899
NlaIII CATG 9 cut(s) 457, 499, 709, 887, 1424, 1625, 1641, 1739, 1828
NlaIV GGNNCC 5 cut(s) 7, 382, 719, 1137, 1408
NmeAIII GCCGAG 1 cut(s) 656
NmuCI GTSAC 6 cut(s) 553, 730, 1054, 1429, 1642, 1667
NspI RCATGY 1 cut(s) 457
NspV TTCGAA 1 cut(s) 926
PaeR7I CTCGAG 2 cut(s) 117, 1275
PaqCI CACCTGC 1 cut(s) 1708
PasI CCCWGGG 1 cut(s) 1397
PceI AGGCCT 1 cut(s) 1761
PcsI WCGNNNNNNNCGW 7 cut(s) 147, 339, 345, 360, 363, 366, 372
PdmI GAANNNNTTC 2 cut(s) 190, 524
PfeI GAWTC 5 cut(s) 409, 549, 842, 880, 1750
PkrI GCNGC 7 cut(s) 20, 263, 667, 712, 781, 1029, 1248
PleI GAGTC 8 cut(s) 32, 668, 993, 1132, 1153, 1583, 1810, 1844
PpsI GAGTC 8 cut(s) 32, 668, 993, 1132, 1153, 1583, 1810, 1844
PshAI GACNNNNGTC 1 cut(s) 737
Psp6I CCWGG 2 cut(s) 1396, 1562
PspFI CCCAGC 1 cut(s) 1402
PspGI CCWGG 2 cut(s) 1396, 1562
PspN4I GGNNCC 5 cut(s) 7, 382, 719, 1137, 1408
PspPI GGNCC 3 cut(s) 430, 739, 1477
PstI CTGCAG 1 cut(s) 1251
PstNI CAGNNNCTG 1 cut(s) 1030
PsuI RGATCY 3 cut(s) 380, 1135, 1785
PvuII CAGCTG 1 cut(s) 1030
RsaI GTAC 5 cut(s) 107, 182, 542, 590, 1459
RsaNI GTAC 5 cut(s) 106, 181, 541, 589, 1458
RseI CAYNNNNRTG 2 cut(s) 452, 1425
Rsr2I CGGWCCG 1 cut(s) 739
RsrII CGGWCCG 1 cut(s) 739
SalI GTCGAC 2 cut(s) 335, 1147
SaqAI TTAA 1 cut(s) 597
SatI GCNGC 7 cut(s) 19, 262, 666, 711, 780, 1028, 1247
Sau3AI GATC 9 cut(s) 86, 145, 285, 380, 475, 1135, 1578, 1785, 1899
Sau96I GGNCC 3 cut(s) 430, 739, 1477
SchI GAGTC 8 cut(s) 32, 668, 994, 1133, 1154, 1583, 1811, 1844
ScrFI CCNGG 3 cut(s) 715, 1398, 1564
SduI GDGCHC 3 cut(s) 517, 722, 1735
SfaNI GCATC 2 cut(s) 966, 1273
SfcI CTRYAG 1 cut(s) 1247
Sfr274I CTCGAG 2 cut(s) 117, 1275
SfuI TTCGAA 1 cut(s) 926
SgrDI CGTCGACG 1 cut(s) 335
SinI GGWCC 3 cut(s) 430, 739, 1477
SlaI CTCGAG 2 cut(s) 117, 1275
SmiMI CAYNNNNRTG 2 cut(s) 452, 1425
SmlI CTYRAG 6 cut(s) 117, 176, 413, 596, 1275, 1763
SmoI CTYRAG 6 cut(s) 117, 176, 413, 596, 1275, 1763
SseBI AGGCCT 1 cut(s) 1761
SsiI CCGC 7 cut(s) 19, 219, 261, 665, 668, 710, 742
SspMI CTAG 3 cut(s) 438, 501, 1227
StuI AGGCCT 1 cut(s) 1761
StyD4I CCNGG 3 cut(s) 713, 1396, 1562
TaaI ACNGT 5 cut(s) 405, 632, 914, 1353, 1684
TaiI ACGT 3 cut(s) 336, 487, 588
TaqI TCGA 9 cut(s) 118, 150, 336, 909, 926, 932, 1148, 1276, 1898
TauI GCSGC 4 cut(s) 21, 264, 668, 713
TfiI GAWTC 5 cut(s) 409, 549, 842, 880, 1750
Tru1I TTAA 1 cut(s) 597
Tru9I TTAA 1 cut(s) 597
TseFI GTSAC 6 cut(s) 553, 730, 1054, 1429, 1642, 1667
TseI GCWGC 3 cut(s) 779, 1027, 1246
Tsp45I GTSAC 6 cut(s) 553, 730, 1054, 1429, 1642, 1667
TspDTI ATGAA 6 cut(s) 484, 872, 1610, 1752, 1813, 1901
TspGWI ACGGA 3 cut(s) 198, 620, 1073
Vha464I CTTAAG 1 cut(s) 596
VpaK11BI GGWCC 3 cut(s) 430, 739, 1477
XagI CCTNNNNNAGG 2 cut(s) 1372, 1793
XapI RAATTY 6 cut(s) 521, 560, 865, 905, 1234, 1285
XbaI TCTAGA 1 cut(s) 500
XceI RCATGY 1 cut(s) 457
XcmI CCANNNNNNNNNTGG 1 cut(s) 1248
XhoI CTCGAG 2 cut(s) 117, 1275
XmiI GTMKAC 2 cut(s) 336, 1148
XmnI GAANNNNTTC 2 cut(s) 190, 524
XspI CTAG 3 cut(s) 438, 501, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.