Rroxscaffold_7G00208510

VID27 cytoplasmic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013857
Physical Location & Seq
Forward (+)
58508892 .. 58512409
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00208510.1

Sequence Viewer

Length: 951 bp
ATGATGCTTATGAGTCCACTGAAGGAGGGAAAGCCTCAGGCAAATGGGCTTCAGCAGCTGGACATTGAAACTGGGAAGATTGTCACGGAATGGAAGTTTGAGAAGGATGGAACAGAGATTACGATGAGGGATATCACCAATGACACTAAGGAGTCGCAATTGGATCCTTCTGAGTCAACATTTCTGGGTTTGGATGATAACAGGCTTTGCCAGTGGGATATGCGTGAACGCAGAGGAATGGTTCAGAACATTGCTAGTTTGAATTCGCCGGTGCTGCAGTGGTCTCAGGGGCATCAGTTTTCTCGTGGGACCAATTTTCAGTGCTTTGCTACTACTGGAGATGGGTCTATTGTTGTTGGGTCTCTGGATGGGAAGATACGGTTGTATTCAAAGACCTCTATGAGGCAGGCAAAGACTGCTTTCCCAGGGCTGGGTTCCCCCATTACTCATGTTGATGTGACTTATGATGGGAAGTGGGTGTTGGGTACAACTGACACTTACTTGGTCCTTATATGCACTCTCTTCACTGACAAAGATGGAAAGACCAAGACTGGGTTTAGTGGTAGAATGGGAAACAAGATACCAGCTCCCAGGTTGTTGAAGCTGATCCCTCTTGACTCACATTTGGCTGGTGCAGACAATAAGTTTCATGGAGGCCACTTCTCATGGGTCACTGAAAGTGGTAAACAAGAGCGTCACCTTGTTGCAACAGTGGGAAAGTTCAGTGTGATATGGGATTTCCAGCAGGTGAAGAATAGTGCCCATGAATGTTACAGGAATCAGCAAGGCCTCAAGAGTTGTTACTGCTACAAGATCCTTTTGAAGGACGAGTCCATTGTGGAGAGCCGTTTCATGCACGATAAGTATGCTGTCAGTGACTCCCCTGAAGCTCCTCTGGTGGTGGCAACCCCGATGAAAGTCAGCTCGATCAGTCTCTCTGGCAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VID27

Protein Analysis

316

Amino Acids

35.27

Weight (kDa)

8.62

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VID27 PF08553 13 - 259 3.5e-40 VID27 C-terminal WD40-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19240
fragaria_vesca FvH4_2g06100
malus_domestica MD05G1080700.v1.1
prunus_persica Prupe.8G124300_v2.0.a1
pyrus_communis pycom05g07340
rosa_chinensis RchiOBHm_Chr6g0257121
rosa_laevigata RLG00000014677
rosa_multiflora Rmu_co8449469.1_g000001 Rmu_sc0000659.1_g000002
rosa_roxburghii Rroxscaffold_7G00208510
rosa_rugosa Rorug05G0575200
rosa_samantha Rh2BG009300 Rh6AG093100 Rh6BG084000 Rh6CG080400 Rh6DG075800
rosa_wichuraiana Rw6G008010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 736
Acc36I ACCTGC 1 cut(s) 736
AclWI GGATC 4 cut(s) 158, 171, 601, 808
AcsI RAATTY 1 cut(s) 262
AcuI CTGAAG 3 cut(s) 35, 41, 906
AfaI GTAC 1 cut(s) 487
AfiI CCNNNNNNNGG 5 cut(s) 402, 430, 431, 552, 823
AgsI TTSAA 5 cut(s) 68, 262, 390, 601, 823
AjnI CCWGG 2 cut(s) 424, 590
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
AluBI AGCT 5 cut(s) 58, 587, 604, 890, 924
AluI AGCT 5 cut(s) 58, 587, 604, 890, 924
Alw26I GTCTC 3 cut(s) 288, 366, 938
AlwI GGATC 4 cut(s) 158, 171, 601, 808
AlwNI CAGNNNCTG 1 cut(s) 58
AoxI GGCC 2 cut(s) 655, 787
ApeKI GCWGC 2 cut(s) 55, 274
ApoI RAATTY 1 cut(s) 262
AspS9I GGNCC 2 cut(s) 309, 505
AsuHPI GGTGA 3 cut(s) 127, 689, 760
AvaII GGWCC 2 cut(s) 309, 505
AxyI CCTNAGG 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 763
BaeI ACNNNNGTAYC 2 cut(s) 368, 401
BamHI GGATCC 1 cut(s) 163
BauI CACGAG 1 cut(s) 303
BbvI GCAGC 2 cut(s) 67, 261
BccI CCATC 5 cut(s) 101, 335, 362, 461, 530
BceAI ACGGC 1 cut(s) 831
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 2 cut(s) 426, 592
BcoDI GTCTC 3 cut(s) 288, 366, 938
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 275
BfuAI ACCTGC 1 cut(s) 736
BisI GCNGC 2 cut(s) 56, 275
BlsI GCNGC 2 cut(s) 57, 276
Bme1390I CCNGG 2 cut(s) 426, 592
Bme18I GGWCC 2 cut(s) 309, 505
BmgT120I GGNCC 2 cut(s) 309, 505
BmiI GGNNCC 3 cut(s) 165, 310, 436
BmrFI CCNGG 2 cut(s) 426, 592
BmrI ACTGGG 2 cut(s) 81, 561
BmsI GCATC 1 cut(s) 301
BmuI ACTGGG 2 cut(s) 81, 561
BpmI CTGGAG 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 776
BsaI GGTCTC 2 cut(s) 288, 366
BsaJI CCNNGG 3 cut(s) 424, 425, 590
Bsc4I CCNNNNNNNGG 5 cut(s) 402, 430, 431, 552, 823
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 4 cut(s) 76, 211, 340, 556
Bse21I CCTNAGG 1 cut(s) 36
Bse3DI GCAATG 1 cut(s) 249
BseBI CCWGG 2 cut(s) 426, 592
BseDI CCNNGG 3 cut(s) 424, 425, 590
BseGI GGATG 3 cut(s) 112, 199, 373
BseLI CCNNNNNNNGG 5 cut(s) 402, 430, 431, 552, 823
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 3 cut(s) 50, 162, 299
BseNI ACTGG 4 cut(s) 76, 211, 340, 556
BseRI GAGGAG 1 cut(s) 882
BseSI GKGCMC 1 cut(s) 763
BseXI GCAGC 2 cut(s) 67, 261
BseYI CCCAGC 1 cut(s) 430
BsgI GTGCAG 1 cut(s) 654
BshFI GGCC 2 cut(s) 657, 789
BsiSI CCGG 1 cut(s) 269
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 5 cut(s) 402, 430, 431, 552, 823
BsmAI GTCTC 3 cut(s) 288, 366, 938
BsmFI GGGAC 1 cut(s) 322
BsnI GGCC 2 cut(s) 657, 789
Bso31I GGTCTC 2 cut(s) 288, 366
Bsp1286I GDGCHC 1 cut(s) 763
Bsp143I GATC 4 cut(s) 163, 606, 813, 927
BspANI GGCC 2 cut(s) 657, 789
BspCNI CTCAG 3 cut(s) 49, 163, 298
BspLI GGNNCC 3 cut(s) 165, 310, 436
BspMAI CTGCAG 1 cut(s) 279
BspMI ACCTGC 1 cut(s) 736
BspPI GGATC 4 cut(s) 158, 171, 601, 808
BspTNI GGTCTC 2 cut(s) 288, 366
BsrDI GCAATG 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 4 cut(s) 76, 211, 340, 556
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 3 cut(s) 424, 425, 590
BssMI GATC 4 cut(s) 163, 606, 813, 927
BssSI CACGAG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 303
Bst2UI CCWGG 2 cut(s) 426, 592
Bst4CI ACNGT 2 cut(s) 381, 712
Bst6I CTCTTC 1 cut(s) 527
BstAPI GCANNNNNTGC 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 4 cut(s) 36, 147, 171, 285
BstENI CCTNNNNNAGG 2 cut(s) 400, 821
BstF5I GGATG 3 cut(s) 112, 199, 373
BstKTI GATC 4 cut(s) 166, 609, 816, 930
BstMAI GTCTC 3 cut(s) 288, 366, 938
BstMBI GATC 4 cut(s) 163, 606, 813, 927
BstMWI GCNNNNNNNGC 3 cut(s) 55, 274, 416
BstNI CCWGG 2 cut(s) 426, 592
BstSCI CCNGG 2 cut(s) 424, 590
BstSFI CTRYAG 1 cut(s) 275
BstSLI GKGCMC 1 cut(s) 763
BstV1I GCAGC 2 cut(s) 67, 261
BstX2I RGATCY 2 cut(s) 163, 813
BstYI RGATCY 2 cut(s) 163, 813
Bsu36I CCTNAGG 1 cut(s) 36
BsuRI GGCC 2 cut(s) 657, 789
BtsCI GGATG 3 cut(s) 112, 199, 373
BtsI GCAGTG 1 cut(s) 284
BtsIMutI CAGTG 9 cut(s) 17, 218, 284, 326, 525, 672, 717, 730, 880
BveI ACCTGC 1 cut(s) 736
Cac8I GCNNGC 1 cut(s) 408
CaiI CAGNNNCTG 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 2 cut(s) 309, 505
CseI GACGC 1 cut(s) 683
Csp6I GTAC 1 cut(s) 486
CviAII CATG 5 cut(s) 449, 650, 666, 764, 853
CviQI GTAC 1 cut(s) 486
DdeI CTNAG 4 cut(s) 36, 147, 171, 285
DpnI GATC 4 cut(s) 165, 608, 815, 929
DpnII GATC 4 cut(s) 163, 606, 813, 927
Eam1104I CTCTTC 1 cut(s) 527
EarI CTCTTC 1 cut(s) 527
Eco147I AGGCCT 1 cut(s) 789
Eco31I GGTCTC 2 cut(s) 288, 366
Eco32I GATATC 1 cut(s) 133
Eco47I GGWCC 2 cut(s) 309, 505
Eco57I CTGAAG 3 cut(s) 35, 41, 906
Eco81I CCTNAGG 1 cut(s) 36
EcoNI CCTNNNNNAGG 2 cut(s) 400, 821
EcoRI GAATTC 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 424, 590
EcoRV GATATC 1 cut(s) 133
FaeI CATG 5 cut(s) 452, 653, 669, 767, 856
FaqI GGGAC 1 cut(s) 322
FatI CATG 5 cut(s) 448, 649, 665, 763, 852
Fnu4HI GCNGC 2 cut(s) 56, 275
FokI GGATG 3 cut(s) 119, 206, 380
Fsp4HI GCNGC 2 cut(s) 56, 275
FspBI CTAG 1 cut(s) 255
GluI GCNGC 2 cut(s) 56, 275
GsaI CCCAGC 1 cut(s) 434
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 2 cut(s) 657, 789
HapII CCGG 1 cut(s) 269
HgaI GACGC 1 cut(s) 683
Hin1II CATG 5 cut(s) 452, 653, 669, 767, 856
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HinfI GANTC 7 cut(s) 13, 152, 173, 617, 778, 830, 878
HpaII CCGG 1 cut(s) 269
HphI GGTGA 3 cut(s) 127, 689, 760
Hpy166II GTNNAC 4 cut(s) 17, 177, 227, 686
Hpy188I TCNGA 2 cut(s) 172, 246
Hpy188III TCNNGA 3 cut(s) 365, 614, 793
Hpy8I GTNNAC 4 cut(s) 17, 177, 227, 686
HpyAV CCTTC 4 cut(s) 16, 97, 177, 817
HpyCH4III ACNGT 2 cut(s) 381, 712
HpyCH4V TGCA 5 cut(s) 277, 516, 635, 707, 856
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 274, 416
HpyF3I CTNAG 4 cut(s) 36, 147, 171, 285
Hsp92II CATG 5 cut(s) 452, 653, 669, 767, 856
Kzo9I GATC 4 cut(s) 163, 606, 813, 927
LmnI GCTCC 2 cut(s) 592, 895
Lsp1109I GCAGC 2 cut(s) 67, 261
LweI GCATC 1 cut(s) 301
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 7 cut(s) 82, 457, 670, 695, 770, 800, 875
MalI GATC 4 cut(s) 165, 608, 815, 929
MboI GATC 4 cut(s) 163, 606, 813, 927
MboII GAAGA 4 cut(s) 88, 385, 514, 763
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 2 cut(s) 163, 813
MhlI GDGCHC 1 cut(s) 763
MluCI AATT 3 cut(s) 158, 262, 313
MlyI GAGTC 6 cut(s) 22, 161, 182, 611, 839, 872
MslI CAYNNNNRTG 1 cut(s) 453
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 2 cut(s) 426, 592
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 2 cut(s) 426, 592
MwoI GCNNNNNNNGC 3 cut(s) 55, 274, 416
NdeII GATC 4 cut(s) 163, 606, 813, 927
NlaIII CATG 5 cut(s) 452, 653, 669, 767, 856
NlaIV GGNNCC 3 cut(s) 165, 310, 436
NmuCI GTSAC 5 cut(s) 82, 457, 670, 695, 875
PaqCI CACCTGC 1 cut(s) 736
PasI CCCWGGG 1 cut(s) 425
PceI AGGCCT 1 cut(s) 789
PfeI GAWTC 1 cut(s) 778
PkrI GCNGC 2 cut(s) 57, 276
PleI GAGTC 6 cut(s) 21, 160, 181, 611, 838, 872
PpsI GAGTC 6 cut(s) 21, 160, 181, 611, 838, 872
Psp6I CCWGG 2 cut(s) 424, 590
PspFI CCCAGC 1 cut(s) 430
PspGI CCWGG 2 cut(s) 424, 590
PspN4I GGNNCC 3 cut(s) 165, 310, 436
PspPI GGNCC 2 cut(s) 309, 505
PsrI GAACNNNNNNTAC 2 cut(s) 103, 135
PstI CTGCAG 1 cut(s) 279
PstNI CAGNNNCTG 1 cut(s) 58
PsuI RGATCY 2 cut(s) 163, 813
PvuII CAGCTG 1 cut(s) 58
RsaI GTAC 1 cut(s) 487
RsaNI GTAC 1 cut(s) 486
RseI CAYNNNNRTG 1 cut(s) 453
SatI GCNGC 2 cut(s) 56, 275
Sau3AI GATC 4 cut(s) 163, 606, 813, 927
Sau96I GGNCC 2 cut(s) 309, 505
SchI GAGTC 6 cut(s) 22, 161, 182, 611, 839, 872
ScrFI CCNGG 2 cut(s) 426, 592
SduI GDGCHC 1 cut(s) 763
SetI ASST 9 cut(s) 60, 398, 589, 596, 606, 702, 750, 892, 926
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 275
SgrAI CRCCGGYG 1 cut(s) 268
SinI GGWCC 2 cut(s) 309, 505
SmiMI CAYNNNNRTG 1 cut(s) 453
SmlI CTYRAG 1 cut(s) 791
SmoI CTYRAG 1 cut(s) 791
Sse9I AATT 3 cut(s) 158, 262, 313
SseBI AGGCCT 1 cut(s) 789
SspMI CTAG 1 cut(s) 255
StuI AGGCCT 1 cut(s) 789
StyD4I CCNGG 2 cut(s) 424, 590
TaaI ACNGT 2 cut(s) 381, 712
TaqI TCGA 1 cut(s) 926
TasI AATT 3 cut(s) 158, 262, 313
TfiI GAWTC 1 cut(s) 778
TscAI CASTG 9 cut(s) 24, 218, 284, 326, 532, 679, 717, 730, 880
TseFI GTSAC 5 cut(s) 82, 457, 670, 695, 875
TseI GCWGC 2 cut(s) 55, 274
Tsp45I GTSAC 5 cut(s) 82, 457, 670, 695, 875
TspDTI ATGAA 4 cut(s) 638, 780, 841, 929
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 9 cut(s) 24, 218, 284, 326, 532, 679, 717, 730, 880
VpaK11BI GGWCC 2 cut(s) 309, 505
XagI CCTNNNNNAGG 2 cut(s) 400, 821
XapI RAATTY 1 cut(s) 262
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.