Rh2BG009300

VID27 cytoplasmic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013857
Physical Location & Seq
Reverse (-)
657505 .. 659898
2394 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG009300.1

Sequence Viewer

Length: 552 bp
ATGAGGCAGGCAAAGACTGCTTTCCCAGGGCTGGGTTCCCCCATTACTCATGTTGATGTGACTTATGATGGGAAGTGGGTGTTGGGTACAACTGACACTTACTTGGTCCTTATATGCACTCTCTTCACTGACAAAGATGGAAAGACCAAGACTGGGTTTAGTGGTAGAATGGGAAACAAGATACCAGCTCCCAGGTTGTTGAAGCTGATCCCTCTTGACTCACATTTGGCTGGTGCAGACAATAAGTTTCATGGAGGTCACTTCTCATGGGTCACTGAAAGTGGTAAACAAGAGCGTCACCTTGTTGCAACAGTGGGAAAGTTCAGTGTGATATGGGATTTCCAGCAGGTGAAGAATAGTGCCCATGAATGTTACAGGAATCAGCAAGGCCTCAAGAGTTGTTACTGCTACAAGATCCTTTTGAAGGACGAGTCCATTGTGGAGAGCCGTTTCATGCACGATAAGTATGCTGTCAGTGACTCCCCTGAAGCTCCTCTGGTGGTGGCAACCCCGATGAAAGTCAGCTCGATCAGTCTCTCTGGCAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VID27

Protein Analysis

183

Amino Acids

20.32

Weight (kDa)

9.25

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VID27 PF08553 3 - 126 4e-21 VID27 C-terminal WD40-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19240
fragaria_vesca FvH4_2g06100
malus_domestica MD05G1080700.v1.1
prunus_persica Prupe.8G124300_v2.0.a1
pyrus_communis pycom05g07340
rosa_chinensis RchiOBHm_Chr6g0257121
rosa_laevigata RLG00000014677
rosa_multiflora Rmu_co8449469.1_g000001 Rmu_sc0000659.1_g000002
rosa_roxburghii Rroxscaffold_7G00208510
rosa_rugosa Rorug05G0575200
rosa_samantha Rh2BG009300 Rh6AG093100 Rh6BG084000 Rh6CG080400 Rh6DG075800
rosa_wichuraiana Rw6G008010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 337
Acc36I ACCTGC 1 cut(s) 337
AclWI GGATC 2 cut(s) 202, 409
AcuI CTGAAG 1 cut(s) 507
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 4 cut(s) 31, 32, 153, 424
AgsI TTSAA 2 cut(s) 202, 424
AjnI CCWGG 2 cut(s) 25, 191
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
AluBI AGCT 4 cut(s) 188, 205, 491, 525
AluI AGCT 4 cut(s) 188, 205, 491, 525
Alw26I GTCTC 1 cut(s) 539
AlwI GGATC 2 cut(s) 202, 409
AoxI GGCC 1 cut(s) 388
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 2 cut(s) 290, 361
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 364
BccI CCATC 2 cut(s) 62, 131
BceAI ACGGC 1 cut(s) 432
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 2 cut(s) 27, 193
BcoDI GTCTC 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 337
Bme1390I CCNGG 2 cut(s) 27, 193
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 2 cut(s) 27, 193
BmrI ACTGGG 1 cut(s) 162
BmuI ACTGGG 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 377
BsaJI CCNNGG 3 cut(s) 25, 26, 191
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 32, 153, 424
Bse1I ACTGG 1 cut(s) 157
BseBI CCWGG 2 cut(s) 27, 193
BseDI CCNNGG 3 cut(s) 25, 26, 191
BseLI CCNNNNNNNGG 4 cut(s) 31, 32, 153, 424
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 483
BseSI GKGCMC 1 cut(s) 364
BseYI CCCAGC 1 cut(s) 31
BsgI GTGCAG 1 cut(s) 255
BshFI GGCC 1 cut(s) 390
BslI CCNNNNNNNGG 4 cut(s) 31, 32, 153, 424
BsmAI GTCTC 1 cut(s) 539
BsnI GGCC 1 cut(s) 390
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 3 cut(s) 207, 414, 528
BspANI GGCC 1 cut(s) 390
BspLI GGNNCC 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 2 cut(s) 202, 409
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 3 cut(s) 25, 26, 191
BssMI GATC 3 cut(s) 207, 414, 528
Bst2UI CCWGG 2 cut(s) 27, 193
Bst4CI ACNGT 1 cut(s) 313
Bst6I CTCTTC 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 9
BstENI CCTNNNNNAGG 1 cut(s) 422
BstKTI GATC 3 cut(s) 210, 417, 531
BstMAI GTCTC 1 cut(s) 539
BstMBI GATC 3 cut(s) 207, 414, 528
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 2 cut(s) 27, 193
BstSCI CCNGG 2 cut(s) 25, 191
BstSLI GKGCMC 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuRI GGCC 1 cut(s) 390
BtsIMutI CAGTG 5 cut(s) 126, 273, 318, 331, 481
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 1 cut(s) 9
Cfr13I GGNCC 1 cut(s) 106
CseI GACGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 87
CviAII CATG 5 cut(s) 50, 251, 267, 365, 454
CviJI RGCY 8 cut(s) 31, 188, 205, 230, 390, 447, 491, 525
CviKI_1 RGCY 8 cut(s) 31, 188, 205, 230, 390, 447, 491, 525
CviQI GTAC 1 cut(s) 87
DpnI GATC 3 cut(s) 209, 416, 530
DpnII GATC 3 cut(s) 207, 414, 528
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco147I AGGCCT 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 507
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 25, 191
FaeI CATG 5 cut(s) 53, 254, 270, 368, 457
FatI CATG 5 cut(s) 49, 250, 266, 364, 453
GsaI CCCAGC 1 cut(s) 35
HaeIII GGCC 1 cut(s) 390
HgaI GACGC 1 cut(s) 284
Hin1II CATG 5 cut(s) 53, 254, 270, 368, 457
HinfI GANTC 4 cut(s) 218, 379, 431, 479
HphI GGTGA 2 cut(s) 290, 361
Hpy166II GTNNAC 1 cut(s) 287
Hpy188III TCNNGA 2 cut(s) 215, 394
Hpy8I GTNNAC 1 cut(s) 287
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 1 cut(s) 313
HpyCH4V TGCA 4 cut(s) 117, 236, 308, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
Hsp92II CATG 5 cut(s) 53, 254, 270, 368, 457
Kzo9I GATC 3 cut(s) 207, 414, 528
LmnI GCTCC 2 cut(s) 193, 496
MaeIII GTNAC 7 cut(s) 58, 257, 271, 296, 371, 401, 476
MalI GATC 3 cut(s) 209, 416, 530
MboI GATC 3 cut(s) 207, 414, 528
MboII GAAGA 2 cut(s) 115, 364
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 364
MlyI GAGTC 3 cut(s) 212, 440, 473
MnlI CCTC 4 cut(s) 222, 248, 401, 504
MslI CAYNNNNRTG 1 cut(s) 54
MspR9I CCNGG 2 cut(s) 27, 193
MvaI CCWGG 2 cut(s) 27, 193
MwoI GCNNNNNNNGC 1 cut(s) 17
NdeII GATC 3 cut(s) 207, 414, 528
NlaIII CATG 5 cut(s) 53, 254, 270, 368, 457
NlaIV GGNNCC 1 cut(s) 37
NmuCI GTSAC 5 cut(s) 58, 257, 271, 296, 476
PaqCI CACCTGC 1 cut(s) 337
PasI CCCWGGG 1 cut(s) 26
PceI AGGCCT 1 cut(s) 390
PfeI GAWTC 1 cut(s) 379
PleI GAGTC 3 cut(s) 212, 439, 473
PpsI GAGTC 3 cut(s) 212, 439, 473
Psp6I CCWGG 2 cut(s) 25, 191
PspFI CCCAGC 1 cut(s) 31
PspGI CCWGG 2 cut(s) 25, 191
PspN4I GGNNCC 1 cut(s) 37
PspPI GGNCC 1 cut(s) 106
PsuI RGATCY 1 cut(s) 414
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
RseI CAYNNNNRTG 1 cut(s) 54
Sau3AI GATC 3 cut(s) 207, 414, 528
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 3 cut(s) 212, 440, 473
ScrFI CCNGG 2 cut(s) 27, 193
SduI GDGCHC 1 cut(s) 364
SetI ASST 8 cut(s) 190, 197, 207, 259, 303, 351, 493, 527
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 54
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
SseBI AGGCCT 1 cut(s) 390
StuI AGGCCT 1 cut(s) 390
StyD4I CCNGG 2 cut(s) 25, 191
TaaI ACNGT 1 cut(s) 313
TaqI TCGA 1 cut(s) 527
TfiI GAWTC 1 cut(s) 379
TscAI CASTG 5 cut(s) 133, 280, 318, 331, 481
TseFI GTSAC 5 cut(s) 58, 257, 271, 296, 476
Tsp45I GTSAC 5 cut(s) 58, 257, 271, 296, 476
TspDTI ATGAA 4 cut(s) 239, 381, 442, 530
TspRI CASTG 5 cut(s) 133, 280, 318, 331, 481
VpaK11BI GGWCC 1 cut(s) 106
XagI CCTNNNNNAGG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.