RchiOBHm_Chr6g0257121

VID27 cytoplasmic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013857
Physical Location & Seq
Forward (+)
12379919 .. 12383567
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23063

Sequence Viewer

Length: 1899 bp
ATGGGAACCTCCCAGAGCCGCGAGGACTACATCTCCGACTCCGACGACTACAAAGAGGAGGAGGAAGAAGAAGAACGATCCGACGATACAGAAGAGTACCAAGACGCTCTAGAGAAACAGCCCACCCCAATCGGCGATCTCGACGCCAAGCTTCAAGCCTTGAAGCTCAAGTACGGAACTCCTTCCTCTTCCTCCTCCACCCCCTCGTCCGCCGTCTCACGTAACGCCGTCAAGCTCTACCTCCACATCGGCGGCAACGCTCCCAATGCCAAGTGGATCGTCTCCAACAAGCTCACCTCCTTCTCCTTCGTCAAGACCTCCAACGTCGACGGCGACAGCGACGACGACTACGAGGGATCCTCGTCGGATGGTAAAGGACAGTGGATTCTCAAGGTTGCGGGCAAGGTCCGAGCTAGGGTTTCCACCGACATGCAATTGAAGATGTTCGGCGATCAGCGACGTGTGGATTTCGTGTCTAGAGGCGTCTGGGCTCTGAAATTCTTCACCGACGAGCAGTACCGGAGATTCGTCACCGAATTTCAGGACTGCTTGTTTGAGAATGTGTACGGGCTTAAGGCGACGGAGGAGAACAAGGTCAAGATTTACGGTAAGGAGTTTTTGGGGTGGGTGAAGCCGGAGGCGGCGGATGACTCGGCGTGGGAGTATGCTGACGATTATGACATGGCCGCCGGGAGCCCCAATTCGGTGACGCCGGTCCGCTCCAACCAGCAATTGATGGAGGAGTTTGAGGAGGCTGCGAATGGAGGTGTTCAGAGCTTGACGCTTGGTGCATTGGATAATAGTTTCTTGGTGAATGATTCTGGTGTTCAGGTGTATAGGAATTTCAGAGATGGGATTCATGGGAAGGGTGTGGTTGCGAAATTCGACAGTGGGGGTGGTTCGAAATCGACACCGAAGAAGGCTCTGCTTATGAGGGGGGAGACTAATATGATGCTTATGAGTCCACTGAAGGAGGGAAAGCCTCAGGCAAATGGGCTTCAGCAGCTGGACATTGAGACTGGGAAGATTGTCACGGAATGGAAGTTTGAGAAGGATGGGACAGAGATTACAATGAGGGATATCACCAATGACACTAAGGAGTCGCAATTGGATCCTTCTGAGTCGACATTTCTGGGTTTGGATGATAACAGGCTTTGCCAGTGGGATATGCGTGAACGCAGAGGAATGGTTCAGAACATTGCTAGTTTGAATTCGCCAGTGCTGCAGTGGTCTCAGGGGCATCAGTTTTCTCGAGGGACGAATTTTCAGTGCTTTGCTACTACTGGAGATGGGTCTATTGTTGTTGGGTCTCTGGATGGGAAGATACGGTTGTATTCCAAGACCTCTATGAGGCAGGCAAAGACTGCTTTCCCAGGGCTGGGTTCCCCCATTACTCATGTTGATGTGACTTATGATGGGAAGTGGGTGTTGGGTACAACTGACACTTACTTGGTCCTTATATGCACTCTCTTCACTGACAAAGATGGAAAGACCAAGACTGGGTTTAGTGGTAGAATGGGAAACAAGATACCAGCTCCCAGGTTGTTGAAGCTGATCCCTCTTGACTCACATTTGGCTGGTGCAGACAATAAGTTTCATGGAGGCCACTTCTCATGGGTCACTGAAAGTGGTAAACAAGAGCGTCACCTTGTTGCAACAGTGGGAAAGTTCAGTGTGATATGGGATTTCCAGCAGGTGAAGAATAGTGCCCATGAATGTTACAGGAATCAGCAAGGCCTCAAGAGTTGTTACTGCTACAAGATCCTTTTGAAGGATGAGTCCATTGTGGAGAGCCGTTTCATGCACGATAAGTATGCTGTCAGTGACTCCCCTGAAGCTCCTCTGGTGGTGGCAACCCCGATGAAAGTCAGCTCGATCAGTCTCTCTGGCAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VID27

Protein Analysis

632

Amino Acids

70.29

Weight (kDa)

5.53

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7135 PF23581 39 - 203 1.4e-71 Domain of unknown function (DUF7135)
VID27 PF08553 260 - 575 9.7e-46 VID27 C-terminal WD40-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19240
fragaria_vesca FvH4_2g06100
malus_domestica MD05G1080700.v1.1
prunus_persica Prupe.8G124300_v2.0.a1
pyrus_communis pycom05g07340
rosa_chinensis RchiOBHm_Chr6g0257121
rosa_laevigata RLG00000014677
rosa_multiflora Rmu_co8449469.1_g000001 Rmu_sc0000659.1_g000002
rosa_roxburghii Rroxscaffold_7G00208510
rosa_rugosa Rorug05G0575200
rosa_samantha Rh2BG009300 Rh6AG093100 Rh6BG084000 Rh6CG080400 Rh6DG075800
rosa_wichuraiana Rw6G008010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1684
Acc36I ACCTGC 1 cut(s) 1684
AccBSI CCGCTC 1 cut(s) 720
AccI GTMKAC 2 cut(s) 327, 1124
AccII CGCG 1 cut(s) 21
AciI CCGC 8 cut(s) 19, 210, 252, 398, 641, 644, 687, 718
AclWI GGATC 8 cut(s) 72, 284, 351, 364, 1106, 1119, 1549, 1756
AcoI YGGCCR 1 cut(s) 684
AcsI RAATTY 6 cut(s) 497, 536, 841, 881, 1210, 1261
AcuI CTGAAG 3 cut(s) 983, 989, 1854
AcyI GRCGYC 3 cut(s) 144, 483, 710
AfaI GTAC 5 cut(s) 98, 173, 518, 566, 1435
AfiI CCNNNNNNNGG 7 cut(s) 415, 703, 1350, 1378, 1379, 1500, 1771
AflII CTTAAG 1 cut(s) 572
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 6 cut(s) 155, 163, 439, 1210, 1549, 1771
AjiI CACGTC 1 cut(s) 461
AjnI CCWGG 2 cut(s) 1372, 1538
AjuI GAANNNNNNNTTGG 2 cut(s) 1412, 1444
Alw26I GTCTC 7 cut(s) 220, 286, 935, 1010, 1236, 1314, 1886
AlwI GGATC 8 cut(s) 72, 284, 351, 364, 1106, 1119, 1549, 1756
AlwNI CAGNNNCTG 1 cut(s) 1006
Ama87I CYCGRG 1 cut(s) 1251
AoxI GGCC 3 cut(s) 684, 1603, 1735
ApeKI GCWGC 3 cut(s) 755, 1003, 1222
ApoI RAATTY 6 cut(s) 497, 536, 841, 881, 1210, 1261
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
Asp700I GAANNNNTTC 3 cut(s) 181, 443, 500
AspS9I GGNCC 3 cut(s) 406, 715, 1453
AsuC2I CCSGG 1 cut(s) 691
AsuHPI GGTGA 9 cut(s) 286, 496, 523, 640, 718, 823, 1075, 1637, 1708
AsuII TTCGAA 1 cut(s) 902
AvaI CYCGRG 1 cut(s) 1251
AvaII GGWCC 3 cut(s) 406, 715, 1453
AxyI CCTNAGG 1 cut(s) 984
BaeGI GKGCMC 1 cut(s) 1711
BaeI ACNNNNGTAYC 2 cut(s) 1316, 1349
BamHI GGATCC 2 cut(s) 356, 1111
BanII GRGCYC 2 cut(s) 493, 698
BarI GAAGNNNNNNTAC 2 cut(s) 155, 187
BbvI GCAGC 3 cut(s) 742, 1015, 1209
BccI CCATC 8 cut(s) 362, 730, 845, 1049, 1283, 1310, 1409, 1478
BceAI ACGGC 4 cut(s) 197, 212, 346, 1779
BcgI CGANNNNNNTGC 2 cut(s) 1796, 1830
BciT130I CCWGG 2 cut(s) 1374, 1540
BcnI CCSGG 1 cut(s) 691
BcoDI GTCTC 7 cut(s) 220, 286, 935, 1010, 1236, 1314, 1886
BfaI CTAG 4 cut(s) 110, 414, 477, 1203
BfmI CTRYAG 1 cut(s) 1223
BfrI CTTAAG 1 cut(s) 572
BfuAI ACCTGC 1 cut(s) 1684
BisI GCNGC 7 cut(s) 19, 253, 642, 687, 756, 1004, 1223
BlsI GCNGC 7 cut(s) 20, 254, 643, 688, 757, 1005, 1224
Bme1390I CCNGG 3 cut(s) 691, 1374, 1540
Bme18I GGWCC 3 cut(s) 406, 715, 1453
BmeT110I CYCGRG 1 cut(s) 1251
BmgBI CACGTC 1 cut(s) 461
BmgT120I GGNCC 3 cut(s) 406, 715, 1453
BmiI GGNNCC 5 cut(s) 7, 358, 695, 1113, 1384
BmrFI CCNGG 3 cut(s) 691, 1374, 1540
BmrI ACTGGG 2 cut(s) 1029, 1509
BmsI GCATC 2 cut(s) 942, 1249
BmuI ACTGGG 2 cut(s) 1029, 1509
BoxI GACNNNNGTC 1 cut(s) 713
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 1 cut(s) 1305
Bpu14I TTCGAA 1 cut(s) 902
BpuEI CTTGAG 3 cut(s) 152, 374, 1724
BpuMI CCSGG 1 cut(s) 691
BsaAI YACGTR 1 cut(s) 221
BsaHI GRCGYC 3 cut(s) 144, 483, 710
BsaI GGTCTC 2 cut(s) 1236, 1314
BsaJI CCNNGG 3 cut(s) 1372, 1373, 1538
BsaWI WCCGGW 1 cut(s) 519
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 7 cut(s) 415, 703, 1350, 1378, 1379, 1500, 1771
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 5 cut(s) 1024, 1159, 1217, 1288, 1504
Bse21I CCTNAGG 1 cut(s) 984
Bse3DI GCAATG 1 cut(s) 1197
BseBI CCWGG 2 cut(s) 1374, 1540
BseDI CCNNGG 3 cut(s) 1372, 1373, 1538
BseGI GGATG 6 cut(s) 373, 652, 1060, 1147, 1321, 1780
BseLI CCNNNNNNNGG 7 cut(s) 415, 703, 1350, 1378, 1379, 1500, 1771
BseMI GCAATG 1 cut(s) 1197
BseMII CTCAG 3 cut(s) 998, 1110, 1247
BseNI ACTGG 5 cut(s) 1024, 1159, 1217, 1288, 1504
BseRI GAGGAG 7 cut(s) 71, 74, 184, 599, 755, 764, 1830
BseSI GKGCMC 1 cut(s) 1711
BseXI GCAGC 3 cut(s) 742, 1015, 1209
BseYI CCCAGC 1 cut(s) 1378
BsgI GTGCAG 1 cut(s) 1602
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 3 cut(s) 686, 1605, 1737
BsiHKCI CYCGRG 1 cut(s) 1251
BsiSI CCGG 4 cut(s) 520, 635, 690, 713
BslFI GGGAC 2 cut(s) 1072, 1270
BslI CCNNNNNNNGG 7 cut(s) 415, 703, 1350, 1378, 1379, 1500, 1771
BsmAI GTCTC 7 cut(s) 220, 286, 935, 1010, 1236, 1314, 1886
BsmBI CGTCTC 2 cut(s) 220, 286
BsmFI GGGAC 2 cut(s) 1072, 1270
BsnI GGCC 3 cut(s) 686, 1605, 1737
Bso31I GGTCTC 2 cut(s) 1236, 1314
BsoBI CYCGRG 1 cut(s) 1251
Bsp119I TTCGAA 1 cut(s) 902
Bsp1286I GDGCHC 3 cut(s) 493, 698, 1711
Bsp143I GATC 9 cut(s) 77, 136, 276, 356, 451, 1111, 1554, 1761, 1875
BspACI CCGC 8 cut(s) 19, 210, 252, 398, 641, 644, 687, 718
BspANI GGCC 3 cut(s) 686, 1605, 1737
BspCNI CTCAG 3 cut(s) 997, 1111, 1246
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 5 cut(s) 7, 358, 695, 1113, 1384
BspMAI CTGCAG 1 cut(s) 1227
BspMI ACCTGC 1 cut(s) 1684
BspPI GGATC 8 cut(s) 72, 284, 351, 364, 1106, 1119, 1549, 1756
BspT104I TTCGAA 1 cut(s) 902
BspTI CTTAAG 1 cut(s) 572
BspTNI GGTCTC 2 cut(s) 1236, 1314
BsrBI CCGCTC 1 cut(s) 720
BsrDI GCAATG 1 cut(s) 1197
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 5 cut(s) 1024, 1159, 1217, 1288, 1504
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 3 cut(s) 1372, 1373, 1538
BssMI GATC 9 cut(s) 77, 136, 276, 356, 451, 1111, 1554, 1761, 1875
BssNI GRCGYC 3 cut(s) 144, 483, 710
Bst2UI CCWGG 2 cut(s) 1374, 1540
Bst4CI ACNGT 5 cut(s) 381, 608, 890, 1329, 1660
Bst6I CTCTTC 3 cut(s) 87, 193, 1475
BstACI GRCGYC 3 cut(s) 144, 483, 710
BstAFI CTTAAG 1 cut(s) 572
BstAPI GCANNNNNTGC 1 cut(s) 1364
BstBAI YACGTR 1 cut(s) 221
BstBI TTCGAA 1 cut(s) 902
BstC8I GCNNGC 2 cut(s) 400, 1356
BstDEI CTNAG 4 cut(s) 984, 1095, 1119, 1233
BstENI CCTNNNNNAGG 2 cut(s) 1348, 1769
BstF5I GGATG 6 cut(s) 373, 652, 1060, 1147, 1321, 1780
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 9 cut(s) 80, 139, 279, 359, 454, 1114, 1557, 1764, 1878
BstMAI GTCTC 7 cut(s) 220, 286, 935, 1010, 1236, 1314, 1886
BstMBI GATC 9 cut(s) 77, 136, 276, 356, 451, 1111, 1554, 1761, 1875
BstMWI GCNNNNNNNGC 4 cut(s) 266, 1003, 1222, 1364
BstNI CCWGG 2 cut(s) 1374, 1540
BstNSI RCATGY 1 cut(s) 433
BstPAI GACNNNNGTC 1 cut(s) 713
BstSCI CCNGG 3 cut(s) 689, 1372, 1538
BstSFI CTRYAG 1 cut(s) 1223
BstSLI GKGCMC 1 cut(s) 1711
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 3 cut(s) 742, 1015, 1209
BstX2I RGATCY 3 cut(s) 356, 1111, 1761
BstYI RGATCY 3 cut(s) 356, 1111, 1761
Bsu36I CCTNAGG 1 cut(s) 984
BsuRI GGCC 3 cut(s) 686, 1605, 1737
BtrI CACGTC 1 cut(s) 461
BtsCI GGATG 6 cut(s) 373, 652, 1060, 1147, 1321, 1780
BtsI GCAGTG 1 cut(s) 1232
BveI ACCTGC 1 cut(s) 1684
Cac8I GCNNGC 2 cut(s) 400, 1356
CaiI CAGNNNCTG 1 cut(s) 1006
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 3 cut(s) 406, 715, 1453
CpoI CGGWCCG 1 cut(s) 715
CseI GACGC 6 cut(s) 113, 152, 472, 718, 790, 1631
Csp6I GTAC 5 cut(s) 97, 172, 517, 565, 1434
CspI CGGWCCG 1 cut(s) 715
CviAII CATG 8 cut(s) 430, 682, 860, 1397, 1598, 1614, 1712, 1801
CviQI GTAC 5 cut(s) 97, 172, 517, 565, 1434
DdeI CTNAG 4 cut(s) 984, 1095, 1119, 1233
DpnI GATC 9 cut(s) 79, 138, 278, 358, 453, 1113, 1556, 1763, 1877
DpnII GATC 9 cut(s) 77, 136, 276, 356, 451, 1111, 1554, 1761, 1875
EaeI YGGCCR 1 cut(s) 684
Eam1104I CTCTTC 3 cut(s) 87, 193, 1475
EarI CTCTTC 3 cut(s) 87, 193, 1475
EciI GGCGGA 2 cut(s) 199, 659
Eco147I AGGCCT 1 cut(s) 1737
Eco24I GRGCYC 2 cut(s) 493, 698
Eco31I GGTCTC 2 cut(s) 1236, 1314
Eco32I GATATC 1 cut(s) 1081
Eco47I GGWCC 3 cut(s) 406, 715, 1453
Eco57I CTGAAG 3 cut(s) 983, 989, 1854
Eco81I CCTNAGG 1 cut(s) 984
Eco88I CYCGRG 1 cut(s) 1251
EcoNI CCTNNNNNAGG 2 cut(s) 1348, 1769
EcoRI GAATTC 1 cut(s) 1210
EcoRII CCWGG 2 cut(s) 1372, 1538
EcoRV GATATC 1 cut(s) 1081
EcoT38I GRGCYC 2 cut(s) 493, 698
Esp3I CGTCTC 2 cut(s) 220, 286
FaeI CATG 8 cut(s) 433, 685, 863, 1400, 1601, 1617, 1715, 1804
FaqI GGGAC 2 cut(s) 1072, 1270
FatI CATG 8 cut(s) 429, 681, 859, 1396, 1597, 1613, 1711, 1800
FauI CCCGC 1 cut(s) 391
FblI GTMKAC 2 cut(s) 327, 1124
Fnu4HI GCNGC 7 cut(s) 19, 253, 642, 687, 756, 1004, 1223
FokI GGATG 6 cut(s) 380, 659, 1067, 1154, 1328, 1787
FriOI GRGCYC 2 cut(s) 493, 698
Fsp4HI GCNGC 7 cut(s) 19, 253, 642, 687, 756, 1004, 1223
FspBI CTAG 4 cut(s) 110, 414, 477, 1203
GluI GCNGC 7 cut(s) 19, 253, 642, 687, 756, 1004, 1223
GsaI CCCAGC 1 cut(s) 1382
GsuI CTGGAG 1 cut(s) 1305
HaeIII GGCC 3 cut(s) 686, 1605, 1737
HapII CCGG 4 cut(s) 520, 635, 690, 713
HgaI GACGC 6 cut(s) 113, 152, 472, 718, 790, 1631
Hin1I GRCGYC 3 cut(s) 144, 483, 710
Hin1II CATG 8 cut(s) 433, 685, 863, 1400, 1601, 1617, 1715, 1804
HincII GTYRAC 2 cut(s) 328, 1125
HindII GTYRAC 2 cut(s) 328, 1125
HindIII AAGCTT 1 cut(s) 149
HpaII CCGG 4 cut(s) 520, 635, 690, 713
HphI GGTGA 9 cut(s) 286, 496, 523, 640, 718, 823, 1075, 1637, 1708
Hpy166II GTNNAC 6 cut(s) 328, 565, 965, 1125, 1175, 1634
Hpy8I GTNNAC 6 cut(s) 328, 565, 965, 1125, 1175, 1634
HpyAV CCTTC 9 cut(s) 192, 310, 316, 859, 913, 964, 1045, 1125, 1765
HpyCH4III ACNGT 5 cut(s) 381, 608, 890, 1329, 1660
HpyCH4IV ACGT 3 cut(s) 220, 324, 460
HpyCH4V TGCA 7 cut(s) 433, 791, 1225, 1464, 1583, 1655, 1804
HpyF10VI GCNNNNNNNGC 4 cut(s) 266, 1003, 1222, 1364
HpyF3I CTNAG 4 cut(s) 984, 1095, 1119, 1233
HpySE526I ACGT 3 cut(s) 220, 324, 460
Hsp92I GRCGYC 3 cut(s) 144, 483, 710
Hsp92II CATG 8 cut(s) 433, 685, 863, 1400, 1601, 1617, 1715, 1804
Kzo9I GATC 9 cut(s) 77, 136, 276, 356, 451, 1111, 1554, 1761, 1875
LmnI GCTCC 5 cut(s) 265, 693, 725, 1540, 1843
Lsp1109I GCAGC 3 cut(s) 742, 1015, 1209
LweI GCATC 2 cut(s) 942, 1249
MaeI CTAG 4 cut(s) 110, 414, 477, 1203
MaeII ACGT 3 cut(s) 220, 324, 460
MalI GATC 9 cut(s) 79, 138, 278, 358, 453, 1113, 1556, 1763, 1877
MbiI CCGCTC 1 cut(s) 720
MboI GATC 9 cut(s) 77, 136, 276, 356, 451, 1111, 1554, 1761, 1875
MfeI CAATTG 3 cut(s) 434, 731, 1106
MflI RGATCY 3 cut(s) 356, 1111, 1761
MhlI GDGCHC 3 cut(s) 493, 698, 1711
MlyI GAGTC 8 cut(s) 32, 644, 970, 1109, 1130, 1559, 1787, 1820
MmeI TCCRAC 7 cut(s) 60, 66, 105, 309, 345, 345, 747
MroXI GAANNNNTTC 3 cut(s) 181, 443, 500
MseI TTAA 1 cut(s) 573
MslI CAYNNNNRTG 2 cut(s) 428, 1401
MspA1I CMGCKG 1 cut(s) 1006
MspCI CTTAAG 1 cut(s) 572
MspI CCGG 4 cut(s) 520, 635, 690, 713
MspR9I CCNGG 3 cut(s) 691, 1374, 1540
MunI CAATTG 3 cut(s) 434, 731, 1106
MvaI CCWGG 2 cut(s) 1374, 1540
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 4 cut(s) 266, 1003, 1222, 1364
NciI CCSGG 1 cut(s) 691
NdeII GATC 9 cut(s) 77, 136, 276, 356, 451, 1111, 1554, 1761, 1875
NlaIII CATG 8 cut(s) 433, 685, 863, 1400, 1601, 1617, 1715, 1804
NlaIV GGNNCC 5 cut(s) 7, 358, 695, 1113, 1384
NmeAIII GCCGAG 1 cut(s) 632
NmuCI GTSAC 7 cut(s) 529, 706, 1030, 1405, 1618, 1643, 1823
NspI RCATGY 1 cut(s) 433
NspV TTCGAA 1 cut(s) 902
PaeR7I CTCGAG 1 cut(s) 1251
PaqCI CACCTGC 1 cut(s) 1684
PasI CCCWGGG 1 cut(s) 1373
PceI AGGCCT 1 cut(s) 1737
PcsI WCGNNNNNNNCGW 4 cut(s) 138, 330, 336, 348
PdmI GAANNNNTTC 3 cut(s) 181, 443, 500
PfeI GAWTC 5 cut(s) 385, 525, 818, 856, 1726
PkrI GCNGC 7 cut(s) 20, 254, 643, 688, 757, 1005, 1224
PleI GAGTC 8 cut(s) 32, 644, 969, 1108, 1129, 1559, 1786, 1820
PpsI GAGTC 8 cut(s) 32, 644, 969, 1108, 1129, 1559, 1786, 1820
Ppu21I YACGTR 1 cut(s) 221
PshAI GACNNNNGTC 1 cut(s) 713
Psp6I CCWGG 2 cut(s) 1372, 1538
PspFI CCCAGC 1 cut(s) 1378
PspGI CCWGG 2 cut(s) 1372, 1538
PspN4I GGNNCC 5 cut(s) 7, 358, 695, 1113, 1384
PspPI GGNCC 3 cut(s) 406, 715, 1453
PstI CTGCAG 1 cut(s) 1227
PstNI CAGNNNCTG 1 cut(s) 1006
PsuI RGATCY 3 cut(s) 356, 1111, 1761
PvuII CAGCTG 1 cut(s) 1006
RsaI GTAC 5 cut(s) 98, 173, 518, 566, 1435
RsaNI GTAC 5 cut(s) 97, 172, 517, 565, 1434
RseI CAYNNNNRTG 2 cut(s) 428, 1401
Rsr2I CGGWCCG 1 cut(s) 715
RsrII CGGWCCG 1 cut(s) 715
SalI GTCGAC 2 cut(s) 326, 1123
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 7 cut(s) 19, 253, 642, 687, 756, 1004, 1223
Sau3AI GATC 9 cut(s) 77, 136, 276, 356, 451, 1111, 1554, 1761, 1875
Sau96I GGNCC 3 cut(s) 406, 715, 1453
SchI GAGTC 8 cut(s) 32, 644, 970, 1109, 1130, 1559, 1787, 1820
ScrFI CCNGG 3 cut(s) 691, 1374, 1540
SduI GDGCHC 3 cut(s) 493, 698, 1711
SfaNI GCATC 2 cut(s) 942, 1249
SfcI CTRYAG 1 cut(s) 1223
Sfr274I CTCGAG 1 cut(s) 1251
SfuI TTCGAA 1 cut(s) 902
SgrDI CGTCGACG 1 cut(s) 326
SinI GGWCC 3 cut(s) 406, 715, 1453
SlaI CTCGAG 1 cut(s) 1251
SmiMI CAYNNNNRTG 2 cut(s) 428, 1401
SmlI CTYRAG 5 cut(s) 167, 389, 572, 1251, 1739
SmoI CTYRAG 5 cut(s) 167, 389, 572, 1251, 1739
SseBI AGGCCT 1 cut(s) 1737
SsiI CCGC 8 cut(s) 19, 210, 252, 398, 641, 644, 687, 718
SspMI CTAG 4 cut(s) 110, 414, 477, 1203
StuI AGGCCT 1 cut(s) 1737
StyD4I CCNGG 3 cut(s) 689, 1372, 1538
TaaI ACNGT 5 cut(s) 381, 608, 890, 1329, 1660
TaiI ACGT 3 cut(s) 223, 327, 463
TaqI TCGA 8 cut(s) 141, 327, 885, 902, 908, 1124, 1252, 1874
TauI GCSGC 4 cut(s) 21, 255, 644, 689
TfiI GAWTC 5 cut(s) 385, 525, 818, 856, 1726
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TseFI GTSAC 7 cut(s) 529, 706, 1030, 1405, 1618, 1643, 1823
TseI GCWGC 3 cut(s) 755, 1003, 1222
Tsp45I GTSAC 7 cut(s) 529, 706, 1030, 1405, 1618, 1643, 1823
TspDTI ATGAA 5 cut(s) 848, 1586, 1728, 1789, 1877
TspGWI ACGGA 3 cut(s) 189, 596, 1049
Vha464I CTTAAG 1 cut(s) 572
VpaK11BI GGWCC 3 cut(s) 406, 715, 1453
XagI CCTNNNNNAGG 2 cut(s) 1348, 1769
XapI RAATTY 6 cut(s) 497, 536, 841, 881, 1210, 1261
XbaI TCTAGA 2 cut(s) 109, 476
XceI RCATGY 1 cut(s) 433
XcmI CCANNNNNNNNNTGG 1 cut(s) 1224
XhoI CTCGAG 1 cut(s) 1251
XmiI GTMKAC 2 cut(s) 327, 1124
XmnI GAANNNNTTC 3 cut(s) 181, 443, 500
XspI CTAG 4 cut(s) 110, 414, 477, 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.