MD05G1086900.v1.1

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014943
Physical Location & Seq
Forward (+)
18527313 .. 18530166
2854 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1086900.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGAAACGACAATCCTCAAGATCTTCTGGGGCCATGGCCTCATTGCCCTGCATGCTCCTTCTCCTCCTCCTCCCCCTTGCCGCCGCCATGGACATGTCCATCGTCGACTACAACGAGAAGCATGGCATGCCCGCCTCGTCGGACCGAACCGAGACGGAGGTCAAGGCCCTCTACGAGTCCTGGCTCGTCAAGCACGCCAAAAACTACAACGCTTTGGGGGAGAAGGAGCGGAGGTTCGAGATTTTTAAGGATAATCTCAGGTTCATTGACGAGCACAATAACCAGAGCCGGACTTACAAGGTGGGTCTGAACCGGTTCGCCGATCTGACCAACGAGGAGTACCGGTCTGTTTACTTGGGTGCCAAGGTGGACCGGCGGTCCCGGCTTTCTGGGTCGAGGAAGAGCGACCGGTACGCGTTCCGAGCCGGCGATAAGTTGCCGGAGTCGGTCGATTGGAGAGCCGAGGGCGCTGTTCCTGCCGTGAAGGATCAGGGCCAATGCGGGAGTTGCTGGGCGTTCTCAACGGTTGGCGCAGTGGAAGGCATAAACAAGATTGTGACCGGTGAGTTGATCTCTTTATCAGAGCAAGAACTAGTGGATTGTGACAGATCATACAACCAAGGATGCAATGGAGGTCTTATGGACTATGCCTTCCAGTTTATCATCAACAATGGCGGTATTGATACTGAAGCAGATTACCCTTACCATGCTCGAGATGGTTCATGTGATCCTAACAGGAAAAATGCTCGGGTTGTGTCCATTGATGGGTATGAAGATATTCCAGAAAATGATGAGAAGTCGTTGAAGAAGGCTGTGGCACACCAACCAGTTAGTGTTGCCATTGAAGCTGGTGGCAGGGAATTCCAACTCTACCAATCAGGTGTCTTCACTGGACGTTGTGGAACAGATCTAGATCACGGTGTGGTTGCTGTTGGTTATGGCACCGAAAATGGTGTAGACTACTGGATCGTGAGGAACTCATGGGGTCCCAATTGGGGCGAGGCTGGTTACATCAGGTTGGAACGTAATGTGGCTAGCACCAATACCGGCAAATGTGGTATTGCCATTGAGGCCTCATACCCTACTAAAAAGGGTCAGAACCCCCCAAGACCTCTCCCATCACCACCATCTCCTCCTCCTGTGAAGCCCCCTACCGTATGCGATGAGTACTACTCATGCCCCTTGGGAACTACATGCTGCTGCGCCTATGAGTATGGCAACTACTGCTTTGGATGGGGATGCTGCCCTCTAGAGTCTGCAACCTGCTGTGATGACCATTACAGCTGCTGTCCTCACGAGTACCCCGTATGCGACCTTGATGCGGGAACTTGCCGATTGCAAGGACAATCCATTGAGTGTGAAAGCGCTAAGGCGAACTCCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000932 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004842 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005764 GO:0005773 GO:0005829 GO:0005911 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009888 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010494 GO:0010498 GO:0010605 GO:0016020 GO:0016049 GO:0016567 GO:0016740 GO:0016787 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021700 GO:0022622 GO:0030054 GO:0030154 GO:0030163 GO:0031146 GO:0031224 GO:0031225 GO:0031347 GO:0031348 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0035770 GO:0036211 GO:0036464 GO:0040007 GO:0042176 GO:0042177 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0048046 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051603 GO:0051704 GO:0051707 GO:0055044 GO:0060255 GO:0060560 GO:0065007 GO:0070011 GO:0070647 GO:0071695 GO:0071704 GO:0080090 GO:0080134 GO:0080147 GO:0090558 GO:0090627 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

462

Amino Acids

50.82

Weight (kDa)

5.31

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 58 - 114 2.1e-19 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 146 - 362 4.2e-88 Papain family cysteine protease
Granulin PF00396 398 - 444 6.5e-09 Granulin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36880
fragaria_vesca FvH4_2g05500
malus_domestica MD05G1086900.v1.1 MD12G1087000.v1.1
prunus_persica Prupe.8G131600_v2.0.a1
pyrus_communis pycom05g08620
rosa_chinensis RchiOBHm_Chr6g0255771
rosa_laevigata RLG00000014814
rosa_multiflora Rmu_sc0001163.1_g000010
rosa_roxburghii Rroxscaffold_7G00209680
rosa_rugosa Rorug05G0561400
rosa_samantha Rh6AG079300 Rh6BG072400 Rh6CG070600 Rh6DG067900
rosa_wichuraiana Rw6G006840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1271
AccB1I GGYRCC 2 cut(s) 359, 941
AccBSI CCGCTC 1 cut(s) 229
AccI GTMKAC 2 cut(s) 105, 957
AccII CGCG 1 cut(s) 416
AciI CCGC 8 cut(s) 81, 84, 132, 229, 376, 501, 675, 1322
AclWI GGATC 3 cut(s) 495, 722, 974
AcsI RAATTY 1 cut(s) 860
AcuI CTGAAG 1 cut(s) 708
AdeI CACNNNGTG 1 cut(s) 922
AfaI GTAC 4 cut(s) 341, 413, 1169, 1301
AfeI AGCGCT 1 cut(s) 1366
AfiI CCNNNNNNNGG 3 cut(s) 765, 995, 1321
AflIII ACRYGT 2 cut(s) 93, 414
AgeI ACCGGT 4 cut(s) 312, 342, 408, 560
AgsI TTSAA 2 cut(s) 805, 845
AhdI GACNNNNNGTC 1 cut(s) 376
AhlI ACTAGT 1 cut(s) 592
AjnI CCWGG 1 cut(s) 179
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 2 cut(s) 848, 1284
AluI AGCT 2 cut(s) 848, 1284
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 1 cut(s) 146
AlwI GGATC 3 cut(s) 495, 722, 974
AlwNI CAGNNNCTG 1 cut(s) 1287
Ama87I CYCGRG 2 cut(s) 711, 747
Aor51HI AGCGCT 1 cut(s) 1366
AoxI GGCC 5 cut(s) 30, 36, 165, 493, 1071
ApeKI GCWGC 4 cut(s) 1197, 1200, 1242, 1284
ApoI RAATTY 1 cut(s) 860
AsiGI ACCGGT 4 cut(s) 312, 342, 408, 560
Asp700I GAANNNNTTC 2 cut(s) 314, 777
AspLEI GCGC 4 cut(s) 470, 533, 1205, 1367
AspS9I GGNCC 7 cut(s) 30, 142, 166, 370, 378, 493, 986
AsuC2I CCSGG 1 cut(s) 382
AsuHPI GGTGA 2 cut(s) 575, 1113
AsuNHI GCTAGC 1 cut(s) 1034
AvaI CYCGRG 2 cut(s) 711, 747
AvaII GGWCC 4 cut(s) 142, 370, 378, 986
BanI GGYRCC 2 cut(s) 359, 941
BauI CACGAG 1 cut(s) 1295
BbsI GAAGAC 1 cut(s) 877
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 4 cut(s) 1184, 1187, 1229, 1271
BccI CCATC 6 cut(s) 107, 710, 758, 1126, 1135, 1227
BceAI ACGGC 1 cut(s) 464
BcgI CGANNNNNNTGC 2 cut(s) 1301, 1335
BciT130I CCWGG 1 cut(s) 181
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 146
BcuI ACTAGT 1 cut(s) 592
BfaI CTAG 5 cut(s) 593, 911, 1035, 1250, 1384
BfoI RGCGCY 2 cut(s) 471, 1368
BfuAI ACCTGC 1 cut(s) 1271
BglI GCCNNNNNGGC 1 cut(s) 1070
BglII AGATCT 2 cut(s) 20, 907
BisI GCNGC 6 cut(s) 81, 84, 1198, 1201, 1243, 1285
BlsI GCNGC 6 cut(s) 82, 85, 1199, 1202, 1244, 1286
BmcAI AGTACT 1 cut(s) 1169
Bme1390I CCNGG 2 cut(s) 181, 382
Bme18I GGWCC 4 cut(s) 142, 370, 378, 986
BmeRI GACNNNNNGTC 1 cut(s) 376
BmeT110I CYCGRG 2 cut(s) 711, 747
BmgT120I GGNCC 7 cut(s) 30, 142, 166, 370, 378, 493, 986
BmiI GGNNCC 6 cut(s) 31, 361, 380, 943, 987, 988
BmrFI CCNGG 2 cut(s) 181, 382
BmsI GCATC 3 cut(s) 614, 1229, 1309
BmtI GCTAGC 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 158
BpiI GAAGAC 1 cut(s) 877
BplI GAGNNNNNCTC 4 cut(s) 557, 589, 1157, 1189
Bpu10I CCTNAGC 1 cut(s) 1368
BpuMI CCSGG 1 cut(s) 382
BsaBI GATNNNNATC 1 cut(s) 912
BsaJI CCNNGG 6 cut(s) 33, 87, 363, 462, 619, 1182
BsaWI WCCGGW 4 cut(s) 312, 342, 408, 560
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 329, 359
Bsc4I CCNNNNNNNGG 3 cut(s) 765, 995, 1321
Bse118I RCCGGY 7 cut(s) 312, 342, 372, 408, 425, 560, 1046
Bse1I ACTGG 4 cut(s) 655, 827, 895, 968
Bse3DI GCAATG 2 cut(s) 41, 634
Bse8I GATNNNNATC 1 cut(s) 912
BseBI CCWGG 1 cut(s) 181
BseDI CCNNGG 6 cut(s) 33, 87, 363, 462, 619, 1182
BseGI GGATG 3 cut(s) 629, 1238, 1244
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 3 cut(s) 765, 995, 1321
BseMI GCAATG 2 cut(s) 41, 634
BseMII CTCAG 1 cut(s) 271
BseNI ACTGG 4 cut(s) 655, 827, 895, 968
BseRI GAGGAG 6 cut(s) 53, 56, 59, 350, 1122, 1125
BseXI GCAGC 4 cut(s) 1184, 1187, 1229, 1271
BseYI CCCAGC 1 cut(s) 510
Bsh1236I CGCG 1 cut(s) 416
Bsh1285I CGRYCG 2 cut(s) 409, 450
BshFI GGCC 5 cut(s) 32, 38, 167, 495, 1073
BshNI GGYRCC 2 cut(s) 359, 941
BshTI ACCGGT 4 cut(s) 312, 342, 408, 560
BsiEI CGRYCG 2 cut(s) 409, 450
BsiHKAI GWGCWC 1 cut(s) 276
BsiHKCI CYCGRG 2 cut(s) 711, 747
BslFI GGGAC 2 cut(s) 364, 972
BslI CCNNNNNNNGG 3 cut(s) 765, 995, 1321
BsmAI GTCTC 1 cut(s) 146
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 2 cut(s) 364, 972
BsnI GGCC 5 cut(s) 32, 38, 167, 495, 1073
BsoBI CYCGRG 2 cut(s) 711, 747
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
Bsp19I CCATGG 2 cut(s) 33, 87
BspACI CCGC 8 cut(s) 81, 84, 132, 229, 376, 501, 675, 1322
BspANI GGCC 5 cut(s) 32, 38, 167, 495, 1073
BspCNI CTCAG 1 cut(s) 270
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 6 cut(s) 31, 361, 380, 943, 987, 988
BspMI ACCTGC 1 cut(s) 1271
BspOI GCTAGC 1 cut(s) 1038
BspPI GGATC 3 cut(s) 495, 722, 974
BspQI GCTCTTC 1 cut(s) 395
BspT107I GGYRCC 2 cut(s) 359, 941
BsrBI CCGCTC 1 cut(s) 229
BsrDI GCAATG 2 cut(s) 41, 634
BsrFI RCCGGY 7 cut(s) 312, 342, 372, 408, 425, 560, 1046
BsrI ACTGG 4 cut(s) 655, 827, 895, 968
BssAI RCCGGY 7 cut(s) 312, 342, 372, 408, 425, 560, 1046
BssECI CCNNGG 6 cut(s) 33, 87, 363, 462, 619, 1182
BssMI GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
BssSI CACGAG 1 cut(s) 1295
BssT1I CCWWGG 5 cut(s) 33, 87, 363, 619, 1182
Bst2BI CACGAG 1 cut(s) 1295
Bst2UI CCWGG 1 cut(s) 181
Bst4CI ACNGT 3 cut(s) 526, 920, 1156
Bst6I CTCTTC 1 cut(s) 395
BstAPI GCANNNNNTGC 2 cut(s) 127, 1224
BstC8I GCNNGC 6 cut(s) 53, 128, 132, 195, 427, 1036
BstDEI CTNAG 2 cut(s) 257, 1368
BstDSI CCRYGG 2 cut(s) 33, 87
BstF5I GGATG 3 cut(s) 629, 1238, 1244
BstFNI CGCG 1 cut(s) 416
BstH2I RGCGCY 2 cut(s) 471, 1368
BstHHI GCGC 4 cut(s) 470, 533, 1205, 1367
BstKTI GATC 9 cut(s) 23, 325, 490, 573, 611, 730, 910, 916, 969
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
BstMCI CGRYCG 2 cut(s) 409, 450
BstNI CCWGG 1 cut(s) 181
BstNSI RCATGY 4 cut(s) 55, 97, 130, 1197
BstPAI GACNNNNGTC 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 179, 380
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 4 cut(s) 1184, 1187, 1229, 1271
BstV2I GAAGAC 1 cut(s) 877
BstX2I RGATCY 2 cut(s) 20, 907
BstYI RGATCY 2 cut(s) 20, 907
BsuRI GGCC 5 cut(s) 32, 38, 167, 495, 1073
BtgI CCRYGG 2 cut(s) 33, 87
BtgZI GCGATG 1 cut(s) 1176
BtsCI GGATG 3 cut(s) 629, 1238, 1244
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 2 cut(s) 540, 888
BveI ACCTGC 1 cut(s) 1271
Cac8I GCNNGC 6 cut(s) 53, 128, 132, 195, 427, 1036
CaiI CAGNNNCTG 1 cut(s) 1287
CfoI GCGC 4 cut(s) 470, 533, 1205, 1367
Cfr10I RCCGGY 7 cut(s) 312, 342, 372, 408, 425, 560, 1046
Cfr13I GGNCC 7 cut(s) 30, 142, 166, 370, 378, 493, 986
CpoI CGGWCCG 1 cut(s) 142
Csp6I GTAC 4 cut(s) 340, 412, 1168, 1300
CspAI ACCGGT 4 cut(s) 312, 342, 408, 560
CspI CGGWCCG 1 cut(s) 142
CviQI GTAC 4 cut(s) 340, 412, 1168, 1300
DdeI CTNAG 2 cut(s) 257, 1368
DpnI GATC 9 cut(s) 22, 324, 489, 572, 610, 729, 909, 915, 968
DpnII GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
DraIII CACNNNGTG 1 cut(s) 922
DriI GACNNNNNGTC 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 395
Eam1105I GACNNNNNGTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 395
Eco130I CCWWGG 5 cut(s) 33, 87, 363, 619, 1182
Eco147I AGGCCT 1 cut(s) 1073
Eco47I GGWCC 4 cut(s) 142, 370, 378, 986
Eco47III AGCGCT 1 cut(s) 1366
Eco57I CTGAAG 1 cut(s) 708
Eco88I CYCGRG 2 cut(s) 711, 747
EcoO109I RGGNCCY 2 cut(s) 166, 986
EcoRI GAATTC 1 cut(s) 860
EcoRII CCWGG 1 cut(s) 179
EcoT14I CCWWGG 5 cut(s) 33, 87, 363, 619, 1182
ErhI CCWWGG 5 cut(s) 33, 87, 363, 619, 1182
Esp3I CGTCTC 1 cut(s) 146
FaqI GGGAC 2 cut(s) 364, 972
FauI CCCGC 3 cut(s) 139, 494, 1315
FblI GTMKAC 2 cut(s) 105, 957
Fnu4HI GCNGC 6 cut(s) 81, 84, 1198, 1201, 1243, 1285
FokI GGATG 3 cut(s) 636, 1245, 1251
Fsp4HI GCNGC 6 cut(s) 81, 84, 1198, 1201, 1243, 1285
FspBI CTAG 5 cut(s) 593, 911, 1035, 1250, 1384
GlaI GCGC 4 cut(s) 469, 532, 1204, 1366
GluI GCNGC 6 cut(s) 81, 84, 1198, 1201, 1243, 1285
GsaI CCCAGC 1 cut(s) 514
HaeII RGCGCY 2 cut(s) 471, 1368
HaeIII GGCC 5 cut(s) 32, 38, 167, 495, 1073
HhaI GCGC 4 cut(s) 470, 533, 1205, 1367
Hin6I GCGC 4 cut(s) 468, 531, 1203, 1365
HinP1I GCGC 4 cut(s) 468, 531, 1203, 1365
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 3 cut(s) 176, 443, 1253
HphI GGTGA 2 cut(s) 575, 1113
Hpy166II GTNNAC 4 cut(s) 106, 352, 370, 958
Hpy188I TCNGA 6 cut(s) 142, 309, 327, 422, 583, 1098
Hpy188III TCNNGA 8 cut(s) 18, 238, 713, 782, 911, 970, 1250, 1295
Hpy8I GTNNAC 4 cut(s) 106, 352, 370, 958
Hpy99I CGWCG 2 cut(s) 107, 142
HpyAV CCTTC 6 cut(s) 68, 217, 478, 533, 661, 802
HpyCH4III ACNGT 3 cut(s) 526, 920, 1156
HpyCH4IV ACGT 2 cut(s) 895, 1024
HpyCH4V TGCA 4 cut(s) 51, 627, 1259, 1339
HpyF3I CTNAG 2 cut(s) 257, 1368
HpySE526I ACGT 2 cut(s) 895, 1024
HspAI GCGC 4 cut(s) 468, 531, 1203, 1365
KflI GGGWCCC 1 cut(s) 986
KroI GCCGGC 1 cut(s) 425
KroNI GCCGGC 1 cut(s) 427
Kzo9I GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
LguI GCTCTTC 1 cut(s) 395
LmnI GCTCC 2 cut(s) 60, 226
Lsp1109I GCAGC 4 cut(s) 1184, 1187, 1229, 1271
LweI GCATC 3 cut(s) 614, 1229, 1309
MaeI CTAG 5 cut(s) 593, 911, 1035, 1250, 1384
MaeII ACGT 2 cut(s) 895, 1024
MaeIII GTNAC 3 cut(s) 556, 602, 1007
MalI GATC 9 cut(s) 22, 324, 489, 572, 610, 729, 909, 915, 968
MbiI CCGCTC 1 cut(s) 229
MboI GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
MboII GAAGA 5 cut(s) 15, 412, 785, 817, 877
MfeI CAATTG 1 cut(s) 991
MflI RGATCY 2 cut(s) 20, 907
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 2 cut(s) 860, 991
MluI ACGCGT 1 cut(s) 414
MlyI GAGTC 3 cut(s) 185, 452, 1262
MmeI TCCRAC 3 cut(s) 120, 889, 999
MroNI GCCGGC 1 cut(s) 425
MroXI GAANNNNTTC 2 cut(s) 314, 777
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 1284
MspR9I CCNGG 2 cut(s) 181, 382
MunI CAATTG 1 cut(s) 991
MvaI CCWGG 1 cut(s) 181
MvnI CGCG 1 cut(s) 416
NaeI GCCGGC 1 cut(s) 427
NciI CCSGG 1 cut(s) 382
NcoI CCATGG 2 cut(s) 33, 87
NdeII GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
NgoMIV GCCGGC 1 cut(s) 425
NheI GCTAGC 1 cut(s) 1034
NlaIV GGNNCC 6 cut(s) 31, 361, 380, 943, 987, 988
NmeAIII GCCGAG 1 cut(s) 487
NmuCI GTSAC 2 cut(s) 556, 602
NspI RCATGY 4 cut(s) 55, 97, 130, 1197
PaeI GCATGC 2 cut(s) 55, 130
PaeR7I CTCGAG 1 cut(s) 711
PceI AGGCCT 1 cut(s) 1073
PciI ACATGT 1 cut(s) 93
PciSI GCTCTTC 1 cut(s) 395
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 1302
PdiI GCCGGC 1 cut(s) 427
PdmI GAANNNNTTC 2 cut(s) 314, 777
PinAI ACCGGT 4 cut(s) 312, 342, 408, 560
PkrI GCNGC 6 cut(s) 82, 85, 1199, 1202, 1244, 1286
PleI GAGTC 3 cut(s) 184, 451, 1261
PpsI GAGTC 3 cut(s) 184, 451, 1261
PpuMI RGGWCCY 1 cut(s) 986
PscI ACATGT 1 cut(s) 93
PshAI GACNNNNGTC 1 cut(s) 158
Psp5II RGGWCCY 1 cut(s) 986
Psp6I CCWGG 1 cut(s) 179
PspFI CCCAGC 1 cut(s) 510
PspGI CCWGG 1 cut(s) 179
PspN4I GGNNCC 6 cut(s) 31, 361, 380, 943, 987, 988
PspPI GGNCC 7 cut(s) 30, 142, 166, 370, 378, 493, 986
PspPPI RGGWCCY 1 cut(s) 986
PstNI CAGNNNCTG 1 cut(s) 1287
PsuI RGATCY 2 cut(s) 20, 907
PvuII CAGCTG 1 cut(s) 1284
RsaI GTAC 4 cut(s) 341, 413, 1169, 1301
RsaNI GTAC 4 cut(s) 340, 412, 1168, 1300
RseI CAYNNNNRTG 1 cut(s) 92
Rsr2I CGGWCCG 1 cut(s) 142
RsrII CGGWCCG 1 cut(s) 142
SalI GTCGAC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 395
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 6 cut(s) 81, 84, 1198, 1201, 1243, 1285
Sau3AI GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
Sau96I GGNCC 7 cut(s) 30, 142, 166, 370, 378, 493, 986
ScaI AGTACT 1 cut(s) 1169
SchI GAGTC 3 cut(s) 185, 452, 1262
ScrFI CCNGG 2 cut(s) 181, 382
SduI GDGCHC 1 cut(s) 276
SfaNI GCATC 3 cut(s) 614, 1229, 1309
Sfr274I CTCGAG 1 cut(s) 711
SinI GGWCC 4 cut(s) 142, 370, 378, 986
SlaI CTCGAG 1 cut(s) 711
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 2 cut(s) 16, 711
SmoI CTYRAG 2 cut(s) 16, 711
SpeI ACTAGT 1 cut(s) 592
SphI GCATGC 2 cut(s) 55, 130
Sse9I AATT 2 cut(s) 860, 991
SseBI AGGCCT 1 cut(s) 1073
SsiI CCGC 8 cut(s) 81, 84, 132, 229, 376, 501, 675, 1322
SspMI CTAG 5 cut(s) 593, 911, 1035, 1250, 1384
StuI AGGCCT 1 cut(s) 1073
StyD4I CCNGG 2 cut(s) 179, 380
StyI CCWWGG 5 cut(s) 33, 87, 363, 619, 1182
TaaI ACNGT 3 cut(s) 526, 920, 1156
TaiI ACGT 2 cut(s) 898, 1027
TaqI TCGA 5 cut(s) 105, 237, 395, 450, 712
TaqII GACCGA 2 cut(s) 159, 436
TasI AATT 2 cut(s) 860, 991
TatI WGTACW 1 cut(s) 1167
TauI GCSGC 2 cut(s) 83, 86
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 2 cut(s) 540, 895
TseFI GTSAC 2 cut(s) 556, 602
TseI GCWGC 4 cut(s) 1197, 1200, 1242, 1284
Tsp45I GTSAC 2 cut(s) 556, 602
TspDTI ATGAA 4 cut(s) 17, 253, 711, 786
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 2 cut(s) 540, 895
VpaK11BI GGWCC 4 cut(s) 142, 370, 378, 986
XapI RAATTY 1 cut(s) 860
XbaI TCTAGA 2 cut(s) 910, 1249
XceI RCATGY 4 cut(s) 55, 97, 130, 1197
XcmI CCANNNNNNNNNTGG 2 cut(s) 626, 713
XhoI CTCGAG 1 cut(s) 711
XmiI GTMKAC 2 cut(s) 105, 957
XmnI GAANNNNTTC 2 cut(s) 314, 777
XspI CTAG 5 cut(s) 593, 911, 1035, 1250, 1384
ZrmI AGTACT 1 cut(s) 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.