Rorug05G0561400

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014943
Physical Location & Seq
Forward (+)
75159354 .. 75198892
39539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0561400.1

Sequence Viewer

Length: 2625 bp
ATGGAAATGAAGGGTTTTGGGTCGATTGATATGTCATCTGTGTTTGTGATTTTTCTGTTATGTGTATGTGGATGTGGTTTGGCTGAGGGTAAGGAGTGTACAAACACTGTTCATCCTGGGATGGCATCACATACTTTACGGTTTGCGCTTTTATCTTCCAATAATGAAAGTTTGAAGAAGGAGATGTTCTCACACTACCACGTGACACCAAGTGATGATATTGCTTGGTTGAATTTGCTCCCAAGAAAGATATTGAAGGAGGAAGATGAATATGAGTGGTCAGTGCTGTACCGGAAGATGAAAATCTCCGCTGGTGGTTCTGAGGCCCCCGGAGGTTTTCTCAAGGAAGTGTCATTGCATGATGTGAGGTTGGATCCAACTTCACAACATGGGAAGGCACAGCAAACAAATCTAGAGTACCTGTTGATGTTGGATTCGGATAGTTTAGTTTGGAGCTTTCGAAAGACAGCTGGTTTAGAAACCCCTGGGGCACCATATGATGCTGAAGGTTCATGGGAGGCATCGACTGGTGAACTGCGGGGTCATTTTGTAGGGCATTATTTGAGTGCTTCAGCAATGATGTGGGCTAGCACTCATAATGATACTCTCAAAGAGAAGATGTCTGGGATTGTGTCAAGTCTCTCTCTTTGTCAGAAGGAAATTGGCAGTGGATATCTTTCGGCTTTTCCAACTGAGGAATTTGATCGCTTTGAAGCTATTACACCTGTCTGGGCTCCTTATTATACCATTCACAAGATGTTGGCAGGCCTGTTTGATCAGTATACACTTGCTGGTAGTGATCAAGCTTTTGATATGATGAAATGGATGGTTGATTACTTTTACAAACGTGTTCAGAATGTGATATCGAAGTACAGTATAGCACACCATTATCTGTCACAAAATGAAGAAACTGGTGGCATGAATGACATTCTTTATAAGTTATATGCTATCACGGGAGATACAAAGCATTTATTGTTGGCTCATCTTTTTGACAAACCCTGCTTTCTAGGAGTGTTGGCAGTGCAGGCTGATGACATATCTGGTTTTCACACCAACACGCATATTCCAGTTGTCATTGGTGCTCAAAAGCGGTATGAAGTCACTGGTGATCCACTTTATAAGGAAATGGGGATTTTCTTTATGGACATGGTTAACTCTTCTCACAGCTATGCAACAGGAGGGACATCAGTGAGCGAGTTTTGGTCAGACCCTAAAAGATTGGCGGCCACTCTACAATCAGAGAATGAGGAATCGTGCACAACTTATAACATGCTGAAGGTCTCTCGCAACCTGTTTAGATGGACCAAAGAAACAGCATATGCGGATTATTACGAGCGAGCTCTAACAAATGGTGTGCTAGGTATCCAAAGAGGAACTGAGCCTGGAGTCATGATCTACATGCTCCCACTAGGTCGTGGACAGTCCAAGGCCAAGAGTTATCATGGTTGGGGATCACCATTTGGCAATTTCTGGTGTTGTTATGGCACTGGGATTGAATCGTTCTCTAAGTTGGGAGATTCCATTTATTTTGAAGAGGAAGGAGAAACTCCTGGTCTTTACATTATCCAGTATATATCAAGCTTGCTTGATTGGAAATCTGGGCAAGTCGTACTTGATCAGACAGTTGATAACATCAATTCCTGGGATCCTTACCTTAGAATCACGTTAACATTTTCTTCAACGAAGGGGACTGGCAAATCATCTACCTTGAACTTGCGAATGCCAATTTGGACTGATTCAGATGGTGCAAAGGCAGCATTGAATGGTCGAAGTCTGCAGCCATCAGCTCCAGGTAGTTTCTTACCAATCCCAAATGAATGGAGCCATGGTGACACAGTAACTCTTCAGCTACCCATTACTCTGAGAACAGAAACTATTAAAGATGATCGGACAGAGTATGCTTCACTCCAAGCAATTCTTTATGGTCCAGTTCTCCTTGCGGCTCATACTAGTGGTAACTGGGACCTCCAAACCAAGTCCAACTCCCTTTCAGACTGGATAACTCCAATCCCTTCCTCGTACAATAACTATTTGGTTTCTTTCTCCCAAGAGTACAAAAACTCTACTTTTGCCTTGGCCAATTTAGACCAGTCGATTGAACTAGACAACTTTCCGGAATCAGGCACTGCTGCTGCTGTTCATGCCACTTTCAGACTCATTTTAGATGACTTGTCAATAAAAGTTCCCAAAGCGCAAGATGCTATTGGTAAATCAGTCATGCTAGAACTGTTTGGTCTTCCTGGGAAGGTTCTTGGGCATCAAAAGAAAGACAAGAACCTTGTGATAGCAGATTCTTCAAGTAGCAAGGAGAACATAGTTTTTCGCTTGGTTCCAGGTTTGGATGGAAAGTCTGCCTCGGTCTCCTTGGAGTCAGAAAGTGCCAAAGGTTGCTTTGTGTATAGCTCGGAGTTGGAGGTTAAAGCAGCCTTGAAGCTCGGTTGCAGTTCAAAATCGTCAAACGATGGATTCAAACAGGCAGCCAGCTTTGTAATGAAGAGTGGAATAAGTCAGTATCATCCTATCAGCTTTGTTGCCCAAGGGAAGCATCGGAATTTTCTTCTACAACCATTGTCAAGCTTCATGGATGAAACTTATACCGTCTATCTCAACATTACAACAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000932 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004842 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005764 GO:0005773 GO:0005829 GO:0005911 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009888 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010494 GO:0010498 GO:0010605 GO:0016020 GO:0016049 GO:0016567 GO:0016740 GO:0016787 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021700 GO:0022622 GO:0030054 GO:0030154 GO:0030163 GO:0031146 GO:0031224 GO:0031225 GO:0031347 GO:0031348 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0035770 GO:0036211 GO:0036464 GO:0040007 GO:0042176 GO:0042177 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0048046 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051603 GO:0051704 GO:0051707 GO:0055044 GO:0060255 GO:0060560 GO:0065007 GO:0070011 GO:0070647 GO:0071695 GO:0071704 GO:0080090 GO:0080134 GO:0080147 GO:0090558 GO:0090627 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

874

Amino Acids

96.96

Weight (kDa)

5.62

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-AFase-like_GH127_cat PF07944 121 - 506 1.4e-107 Beta-L-arabinofuranosidase, GH127 catalytic domain
Glyco_hydro127M PF20736 519 - 619 6.7e-15 Beta-L-arabinofuranosidase, GH127 middle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36880
fragaria_vesca FvH4_2g05500
malus_domestica MD05G1086900.v1.1 MD12G1087000.v1.1
prunus_persica Prupe.8G131600_v2.0.a1
pyrus_communis pycom05g08620
rosa_chinensis RchiOBHm_Chr6g0255771
rosa_laevigata RLG00000014814
rosa_multiflora Rmu_sc0001163.1_g000010
rosa_roxburghii Rroxscaffold_7G00209680
rosa_rugosa Rorug05G0561400
rosa_samantha Rh6AG079300 Rh6BG072400 Rh6CG070600 Rh6DG067900
rosa_wichuraiana Rw6G006840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 936, 1119, 1266
AccB1I GGYRCC 1 cut(s) 490
AccI GTMKAC 1 cut(s) 782
AccIII TCCGGA 1 cut(s) 2113
AciI CCGC 6 cut(s) 309, 538, 1090, 1223, 1322, 1940
AclWI GGATC 6 cut(s) 368, 381, 1103, 1459, 1640, 1653
AcoI YGGCCR 2 cut(s) 1224, 2076
AcsI RAATTY 3 cut(s) 232, 698, 2551
AcuI CTGAAG 4 cut(s) 525, 555, 1295, 1829
AcvI CACGTG 1 cut(s) 202
AdeI CACNNNGTG 1 cut(s) 212
AfaI GTAC 7 cut(s) 100, 290, 419, 872, 1611, 2021, 2054
AfiI CCNNNNNNNGG 1 cut(s) 2120
AflIII ACRYGT 1 cut(s) 847
AhlI ACTAGT 1 cut(s) 1949
AjnI CCWGG 8 cut(s) 115, 484, 1381, 1549, 1640, 1789, 2239, 2332
AjuI GAANNNNNNNTTGG 2 cut(s) 1711, 1743
Alw21I GWGCWC 3 cut(s) 1084, 1259, 1342
Alw26I GTCTC 3 cut(s) 644, 1285, 2365
Alw44I GTGCAC 1 cut(s) 1255
AlwI GGATC 6 cut(s) 368, 381, 1103, 1459, 1640, 1653
AlwNI CAGNNNCTG 1 cut(s) 2126
Aor13HI TCCGGA 1 cut(s) 2113
AoxI GGCC 5 cut(s) 324, 766, 1224, 1428, 2076
ApaLI GTGCAC 1 cut(s) 1255
ApeKI GCWGC 6 cut(s) 1754, 1777, 2129, 2132, 2423, 2477
ApoI RAATTY 3 cut(s) 232, 698, 2551
AspLEI GCGC 2 cut(s) 148, 2194
AspS9I GGNCC 4 cut(s) 325, 1302, 1925, 1963
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 4 cut(s) 542, 1118, 1446, 1841
AsuII TTCGAA 1 cut(s) 460
AsuNHI GCTAGC 1 cut(s) 587
AvaII GGWCC 3 cut(s) 1302, 1925, 1963
BaeGI GKGCMC 2 cut(s) 493, 1259
BalI TGGCCA 1 cut(s) 2078
BamHI GGATCC 2 cut(s) 373, 1645
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 2 cut(s) 736, 1342
BbrPI CACGTG 1 cut(s) 202
BbsI GAAGAC 1 cut(s) 2228
Bbv12I GWGCWC 3 cut(s) 1084, 1259, 1342
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 6 cut(s) 1766, 1789, 2116, 2119, 2435, 2489
BccI CCATC 7 cut(s) 115, 820, 1293, 1736, 1789, 2336, 2456
BciT130I CCWGG 8 cut(s) 117, 486, 1383, 1551, 1642, 1791, 2241, 2334
BciVI GTATCC 1 cut(s) 1373
BclI TGATCA 3 cut(s) 775, 799, 1615
BcnI CCSGG 1 cut(s) 330
BcoDI GTCTC 3 cut(s) 644, 1285, 2365
BcuI ACTAGT 1 cut(s) 1949
BfaI CTAG 8 cut(s) 413, 588, 1007, 1358, 1409, 1950, 2102, 2222
BfmI CTRYAG 1 cut(s) 1775
BfuI GTATCC 1 cut(s) 1373
BisI GCNGC 8 cut(s) 1224, 1755, 1778, 1941, 2130, 2133, 2424, 2478
BlsI GCNGC 8 cut(s) 1225, 1756, 1779, 1942, 2131, 2134, 2425, 2479
Bme1390I CCNGG 9 cut(s) 117, 330, 486, 1383, 1551, 1642, 1791, 2241, 2334
Bme18I GGWCC 3 cut(s) 1302, 1925, 1963
BmgT120I GGNCC 4 cut(s) 325, 1302, 1925, 1963
BmiI GGNNCC 8 cut(s) 327, 375, 492, 735, 1647, 1823, 1964, 2331
BmrFI CCNGG 9 cut(s) 117, 330, 486, 1383, 1551, 1642, 1791, 2241, 2334
BmrI ACTGGG 2 cut(s) 1497, 1969
BmsI GCATC 6 cut(s) 134, 490, 530, 2188, 2266, 2554
BmtI GCTAGC 1 cut(s) 591
BmuI ACTGGG 2 cut(s) 1497, 1969
BpiI GAAGAC 1 cut(s) 2228
BplI GAGNNNNNCTC 4 cut(s) 173, 205, 324, 356
BpmI CTGGAG 2 cut(s) 1404, 1773
Bpu10I CCTNAGC 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 460
BpuEI CTTGAG 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 330
BsaAI YACGTR 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 302
BsaI GGTCTC 2 cut(s) 1285, 2365
BsaWI WCCGGW 2 cut(s) 291, 2113
BsaXI ACNNNNNCTCC 2 cut(s) 1813, 1843
Bsc4I CCNNNNNNNGG 1 cut(s) 2120
Bse3DI GCAATG 2 cut(s) 353, 582
Bse8I GATNNNNATC 1 cut(s) 302
BseAI TCCGGA 1 cut(s) 2113
BseBI CCWGG 8 cut(s) 117, 486, 1383, 1551, 1642, 1791, 2241, 2334
BseGI GGATG 7 cut(s) 77, 112, 126, 831, 2347, 2515, 2590
BseJI GATNNNNATC 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 2120
BseMI GCAATG 2 cut(s) 353, 582
BseMII CTCAG 5 cut(s) 75, 312, 684, 1368, 1853
BseSI GKGCMC 2 cut(s) 493, 1259
BseXI GCAGC 6 cut(s) 1766, 1789, 2116, 2119, 2435, 2489
BsgI GTGCAG 1 cut(s) 1043
BshFI GGCC 5 cut(s) 326, 768, 1226, 1430, 2078
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 3 cut(s) 1084, 1259, 1342
BsiSI CCGG 3 cut(s) 292, 330, 2114
BslFI GGGAC 3 cut(s) 1195, 1702, 1976
BslI CCNNNNNNNGG 1 cut(s) 2120
BsmAI GTCTC 3 cut(s) 644, 1285, 2365
BsmFI GGGAC 3 cut(s) 1195, 1702, 1976
BsmI GAATGC 1 cut(s) 1725
BsnI GGCC 5 cut(s) 326, 768, 1226, 1430, 2078
Bso31I GGTCTC 2 cut(s) 1285, 2365
Bsp119I TTCGAA 1 cut(s) 460
Bsp1286I GDGCHC 5 cut(s) 493, 736, 1084, 1259, 1342
Bsp13I TCCGGA 1 cut(s) 2113
Bsp1407I TGTACA 1 cut(s) 98
Bsp19I CCATGG 1 cut(s) 1825
BspACI CCGC 6 cut(s) 309, 538, 1090, 1223, 1322, 1940
BspANI GGCC 5 cut(s) 326, 768, 1226, 1430, 2078
BspCNI CTCAG 5 cut(s) 76, 313, 685, 1369, 1854
BspEI TCCGGA 1 cut(s) 2113
BspHI TCATGA 1 cut(s) 1389
BspLI GGNNCC 8 cut(s) 327, 375, 492, 735, 1647, 1823, 1964, 2331
BspMAI CTGCAG 1 cut(s) 1779
BspOI GCTAGC 1 cut(s) 591
BspPI GGATC 6 cut(s) 368, 381, 1103, 1459, 1640, 1653
BspT104I TTCGAA 1 cut(s) 460
BspT107I GGYRCC 1 cut(s) 490
BspTNI GGTCTC 2 cut(s) 1285, 2365
BsrDI GCAATG 2 cut(s) 353, 582
BsrGI TGTACA 1 cut(s) 98
BssNAI GTATAC 1 cut(s) 783
BssT1I CCWWGG 5 cut(s) 1425, 1825, 2073, 2364, 2536
Bst1107I GTATAC 1 cut(s) 783
Bst2UI CCWGG 8 cut(s) 117, 486, 1383, 1551, 1642, 1791, 2241, 2334
Bst4CI ACNGT 9 cut(s) 109, 141, 875, 1422, 1624, 1837, 2229, 2599, 2620
Bst6I CTCTTC 4 cut(s) 1162, 1527, 1848, 2489
BstAUI TGTACA 1 cut(s) 98
BstBAI YACGTR 1 cut(s) 202
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 6 cut(s) 589, 766, 1026, 1338, 1583, 2482
BstDEI CTNAG 7 cut(s) 84, 321, 693, 1377, 1506, 1655, 1862
BstDSI CCRYGG 1 cut(s) 1825
BstF5I GGATG 7 cut(s) 77, 112, 126, 831, 2347, 2515, 2590
BstHHI GCGC 2 cut(s) 148, 2194
BstMAI GTCTC 3 cut(s) 644, 1285, 2365
BstMWI GCNNNNNNNGC 4 cut(s) 1025, 1754, 2141, 2198
BstNI CCWGG 8 cut(s) 117, 486, 1383, 1551, 1642, 1791, 2241, 2334
BstNSI RCATGY 2 cut(s) 1273, 1402
BstSCI CCNGG 9 cut(s) 115, 328, 484, 1381, 1549, 1640, 1789, 2239, 2332
BstSFI CTRYAG 1 cut(s) 1775
BstSLI GKGCMC 2 cut(s) 493, 1259
BstV1I GCAGC 6 cut(s) 1766, 1789, 2116, 2119, 2435, 2489
BstV2I GAAGAC 1 cut(s) 2228
BstX2I RGATCY 2 cut(s) 373, 1645
BstXI CCANNNNNNTGG 1 cut(s) 1818
BstYI RGATCY 2 cut(s) 373, 1645
BstZ17I GTATAC 1 cut(s) 783
BsuI GTATCC 1 cut(s) 1373
BsuRI GGCC 5 cut(s) 326, 768, 1226, 1430, 2078
BtgI CCRYGG 1 cut(s) 1825
BtsCI GGATG 7 cut(s) 77, 112, 126, 831, 2347, 2515, 2590
BtsI GCAGTG 3 cut(s) 673, 1026, 2124
BtsIMutI CAGTG 8 cut(s) 105, 288, 673, 1026, 1101, 1194, 1485, 2124
Cac8I GCNNGC 6 cut(s) 589, 766, 1026, 1338, 1583, 2482
CaiI CAGNNNCTG 1 cut(s) 2126
CciI TCATGA 1 cut(s) 1389
CfoI GCGC 2 cut(s) 148, 2194
Cfr13I GGNCC 4 cut(s) 325, 1302, 1925, 1963
Csp6I GTAC 7 cut(s) 99, 289, 418, 871, 1610, 2020, 2053
CviQI GTAC 7 cut(s) 99, 289, 418, 871, 1610, 2020, 2053
DdeI CTNAG 7 cut(s) 84, 321, 693, 1377, 1506, 1655, 1862
DraIII CACNNNGTG 1 cut(s) 212
EaeI YGGCCR 2 cut(s) 1224, 2076
Eam1104I CTCTTC 4 cut(s) 1162, 1527, 1848, 2489
EarI CTCTTC 4 cut(s) 1162, 1527, 1848, 2489
Ecl136II GAGCTC 1 cut(s) 1340
Eco130I CCWWGG 5 cut(s) 1425, 1825, 2073, 2364, 2536
Eco147I AGGCCT 1 cut(s) 768
Eco24I GRGCYC 2 cut(s) 736, 1342
Eco31I GGTCTC 2 cut(s) 1285, 2365
Eco32I GATATC 2 cut(s) 674, 864
Eco47I GGWCC 3 cut(s) 1302, 1925, 1963
Eco53kI GAGCTC 1 cut(s) 1340
Eco57I CTGAAG 4 cut(s) 525, 555, 1295, 1829
Eco72I CACGTG 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 1340
EcoO109I RGGNCCY 2 cut(s) 325, 1963
EcoRII CCWGG 8 cut(s) 115, 484, 1381, 1549, 1640, 1789, 2239, 2332
EcoRV GATATC 2 cut(s) 674, 864
EcoT14I CCWWGG 5 cut(s) 1425, 1825, 2073, 2364, 2536
EcoT38I GRGCYC 2 cut(s) 736, 1342
ErhI CCWWGG 5 cut(s) 1425, 1825, 2073, 2364, 2536
FalI AAGNNNNNCTT 4 cut(s) 1596, 1628, 1902, 1934
FaqI GGGAC 3 cut(s) 1195, 1702, 1976
FauI CCCGC 1 cut(s) 531
FauNDI CATATG 2 cut(s) 496, 1318
FbaI TGATCA 3 cut(s) 775, 799, 1615
FblI GTMKAC 1 cut(s) 782
Fnu4HI GCNGC 8 cut(s) 1224, 1755, 1778, 1941, 2130, 2133, 2424, 2478
FokI GGATG 7 cut(s) 84, 99, 133, 838, 2354, 2502, 2597
FriOI GRGCYC 2 cut(s) 736, 1342
Fsp4HI GCNGC 8 cut(s) 1224, 1755, 1778, 1941, 2130, 2133, 2424, 2478
FspBI CTAG 8 cut(s) 413, 588, 1007, 1358, 1409, 1950, 2102, 2222
GlaI GCGC 2 cut(s) 147, 2193
GluI GCNGC 8 cut(s) 1224, 1755, 1778, 1941, 2130, 2133, 2424, 2478
GsuI CTGGAG 2 cut(s) 1404, 1773
HaeIII GGCC 5 cut(s) 326, 768, 1226, 1430, 2078
HapII CCGG 3 cut(s) 292, 330, 2114
HhaI GCGC 2 cut(s) 148, 2194
Hin6I GCGC 2 cut(s) 146, 2192
HinP1I GCGC 2 cut(s) 146, 2192
HincII GTYRAC 2 cut(s) 1153, 1668
HindII GTYRAC 2 cut(s) 1153, 1668
HindIII AAGCTT 3 cut(s) 804, 1579, 2575
HpaI GTTAAC 2 cut(s) 1153, 1668
HpaII CCGG 3 cut(s) 292, 330, 2114
HphI GGTGA 4 cut(s) 542, 1118, 1446, 1841
Hpy166II GTNNAC 7 cut(s) 99, 533, 783, 1153, 1257, 1418, 1668
Hpy188III TCNNGA 3 cut(s) 413, 1390, 2114
Hpy8I GTNNAC 7 cut(s) 99, 533, 783, 1153, 1257, 1418, 1668
HpyCH4III ACNGT 9 cut(s) 109, 141, 875, 1422, 1624, 1837, 2229, 2599, 2620
HpyCH4IV ACGT 3 cut(s) 201, 847, 1664
HpyCH4V TGCA 7 cut(s) 358, 1024, 1172, 1257, 1748, 1777, 2442
HpyF10VI GCNNNNNNNGC 4 cut(s) 1025, 1754, 2141, 2198
HpyF3I CTNAG 7 cut(s) 84, 321, 693, 1377, 1506, 1655, 1862
HpySE526I ACGT 3 cut(s) 201, 847, 1664
HspAI GCGC 2 cut(s) 146, 2192
Kpn2I TCCGGA 1 cut(s) 2113
Ksp22I TGATCA 3 cut(s) 775, 799, 1615
KspAI GTTAAC 2 cut(s) 1153, 1668
LmnI GCTCC 6 cut(s) 243, 453, 739, 1407, 1792, 1821
Lsp1109I GCAGC 6 cut(s) 1766, 1789, 2116, 2119, 2435, 2489
LweI GCATC 6 cut(s) 134, 490, 530, 2188, 2266, 2554
MaeI CTAG 8 cut(s) 413, 588, 1007, 1358, 1409, 1950, 2102, 2222
MaeII ACGT 3 cut(s) 201, 847, 1664
MaeIII GTNAC 6 cut(s) 202, 894, 1099, 1829, 1837, 1955
MflI RGATCY 2 cut(s) 373, 1645
MhlI GDGCHC 5 cut(s) 493, 736, 1084, 1259, 1342
MlsI TGGCCA 1 cut(s) 2078
MluCI AATT 9 cut(s) 232, 660, 698, 1465, 1636, 1725, 1914, 2080, 2551
MluNI TGGCCA 1 cut(s) 2078
MlyI GAGTC 3 cut(s) 1395, 2148, 2378
MmeI TCCRAC 6 cut(s) 351, 401, 411, 713, 2004, 2391
Mox20I TGGCCA 1 cut(s) 2078
MroI TCCGGA 1 cut(s) 2113
MscI TGGCCA 1 cut(s) 2078
MseI TTAA 5 cut(s) 1152, 1667, 1878, 2418, 2623
MslI CAYNNNNRTG 2 cut(s) 1167, 1950
Msp20I TGGCCA 1 cut(s) 2078
MspA1I CMGCKG 2 cut(s) 311, 470
MspI CCGG 3 cut(s) 292, 330, 2114
MspR9I CCNGG 9 cut(s) 117, 330, 486, 1383, 1551, 1642, 1791, 2241, 2334
Mva1269I GAATGC 1 cut(s) 1725
MvaI CCWGG 8 cut(s) 117, 486, 1383, 1551, 1642, 1791, 2241, 2334
MwoI GCNNNNNNNGC 4 cut(s) 1025, 1754, 2141, 2198
NciI CCSGG 1 cut(s) 330
NcoI CCATGG 1 cut(s) 1825
NdeI CATATG 2 cut(s) 496, 1318
NheI GCTAGC 1 cut(s) 587
NlaIV GGNNCC 8 cut(s) 327, 375, 492, 735, 1647, 1823, 1964, 2331
NmuCI GTSAC 4 cut(s) 202, 894, 1099, 1829
NspI RCATGY 2 cut(s) 1273, 1402
NspV TTCGAA 1 cut(s) 460
PagI TCATGA 1 cut(s) 1389
PasI CCCWGGG 1 cut(s) 485
PceI AGGCCT 1 cut(s) 768
PctI GAATGC 1 cut(s) 1725
PfeI GAWTC 9 cut(s) 434, 1250, 1496, 1517, 1659, 1736, 2117, 2291, 2466
PkrI GCNGC 8 cut(s) 1225, 1756, 1779, 1942, 2131, 2134, 2425, 2479
PleI GAGTC 3 cut(s) 1394, 2148, 2377
PmaCI CACGTG 1 cut(s) 202
PmlI CACGTG 1 cut(s) 202
PpsI GAGTC 3 cut(s) 1394, 2148, 2377
Ppu21I YACGTR 1 cut(s) 202
PpuMI RGGWCCY 1 cut(s) 1963
PsiI TTATAA 3 cut(s) 936, 1119, 1266
Psp124BI GAGCTC 1 cut(s) 1342
Psp5II RGGWCCY 1 cut(s) 1963
Psp6I CCWGG 8 cut(s) 115, 484, 1381, 1549, 1640, 1789, 2239, 2332
PspCI CACGTG 1 cut(s) 202
PspGI CCWGG 8 cut(s) 115, 484, 1381, 1549, 1640, 1789, 2239, 2332
PspN4I GGNNCC 8 cut(s) 327, 375, 492, 735, 1647, 1823, 1964, 2331
PspPI GGNCC 4 cut(s) 325, 1302, 1925, 1963
PspPPI RGGWCCY 1 cut(s) 1963
PstI CTGCAG 1 cut(s) 1779
PstNI CAGNNNCTG 1 cut(s) 2126
PsuI RGATCY 2 cut(s) 373, 1645
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 7 cut(s) 100, 290, 419, 872, 1611, 2021, 2054
RsaNI GTAC 7 cut(s) 99, 289, 418, 871, 1610, 2020, 2053
RseI CAYNNNNRTG 2 cut(s) 1167, 1950
SacI GAGCTC 1 cut(s) 1342
SaqAI TTAA 5 cut(s) 1152, 1667, 1878, 2418, 2623
SatI GCNGC 8 cut(s) 1224, 1755, 1778, 1941, 2130, 2133, 2424, 2478
Sau96I GGNCC 4 cut(s) 325, 1302, 1925, 1963
SchI GAGTC 3 cut(s) 1395, 2148, 2378
ScrFI CCNGG 9 cut(s) 117, 330, 486, 1383, 1551, 1642, 1791, 2241, 2334
SduI GDGCHC 5 cut(s) 493, 736, 1084, 1259, 1342
SfaNI GCATC 6 cut(s) 134, 490, 530, 2188, 2266, 2554
SfcI CTRYAG 1 cut(s) 1775
SfuI TTCGAA 1 cut(s) 460
SinI GGWCC 3 cut(s) 1302, 1925, 1963
SmiMI CAYNNNNRTG 2 cut(s) 1167, 1950
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SpeI ACTAGT 1 cut(s) 1949
Sse9I AATT 9 cut(s) 232, 660, 698, 1465, 1636, 1725, 1914, 2080, 2551
SseBI AGGCCT 1 cut(s) 768
SsiI CCGC 6 cut(s) 309, 538, 1090, 1223, 1322, 1940
SspMI CTAG 8 cut(s) 413, 588, 1007, 1358, 1409, 1950, 2102, 2222
SstI GAGCTC 1 cut(s) 1342
StuI AGGCCT 1 cut(s) 768
StyD4I CCNGG 9 cut(s) 115, 328, 484, 1381, 1549, 1640, 1789, 2239, 2332
StyI CCWWGG 5 cut(s) 1425, 1825, 2073, 2364, 2536
TaaI ACNGT 9 cut(s) 109, 141, 875, 1422, 1624, 1837, 2229, 2599, 2620
TaiI ACGT 3 cut(s) 204, 850, 1667
TaqI TCGA 6 cut(s) 23, 460, 524, 866, 1768, 2093
TaqII GACCGA 1 cut(s) 2347
TasI AATT 9 cut(s) 232, 660, 698, 1465, 1636, 1725, 1914, 2080, 2551
TatI WGTACW 3 cut(s) 98, 870, 2052
TauI GCSGC 2 cut(s) 1226, 1943
TfiI GAWTC 9 cut(s) 434, 1250, 1496, 1517, 1659, 1736, 2117, 2291, 2466
Tru1I TTAA 5 cut(s) 1152, 1667, 1878, 2418, 2623
Tru9I TTAA 5 cut(s) 1152, 1667, 1878, 2418, 2623
TscAI CASTG 8 cut(s) 112, 288, 673, 1026, 1108, 1194, 1492, 2131
TseFI GTSAC 4 cut(s) 202, 894, 1099, 1829
TseI GCWGC 6 cut(s) 1754, 1777, 2129, 2132, 2423, 2477
Tsp45I GTSAC 4 cut(s) 202, 894, 1099, 1829
TspRI CASTG 8 cut(s) 112, 288, 673, 1026, 1108, 1194, 1492, 2131
VneI GTGCAC 1 cut(s) 1255
VpaK11BI GGWCC 3 cut(s) 1302, 1925, 1963
XapI RAATTY 3 cut(s) 232, 698, 2551
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 2 cut(s) 1273, 1402
XmiI GTMKAC 1 cut(s) 782
XspI CTAG 8 cut(s) 413, 588, 1007, 1358, 1409, 1950, 2102, 2222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.