MD12G1087000.v1.1

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014943
Physical Location & Seq
Forward (+)
10676269 .. 10678161
1893 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1087000.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGAAACGACAATCCTTAAGATCTTCTGTGGCCATGGCCTCATTGGCCTGCATGGCCCTCCTCATCCTCCTCCCCCTCGTGGCCGCCATGGACGTGTCCATCATCGACTACAATGACAAGCACGGCATGACCACCCCGTCGGACCGGACCGAGCCGGAGGCGAAAGCCCTCTACGAGTCCTGGCTCATCAAGCACGGCAAAAATTACAACGCTTTGGGCGAGAAGGAGCGGAGATTCGAGATTTTTAAGGATAATCTGAGGTTCATCGACGAGCACAACCTACAGAGCCGGACTTACAAGGTGGGTCTAAACCGGTTTGCCGATCTGACCAACGAGGAGTACCGGTCTGTTTACTTGGTCGCCAAGGTGGACCGGGGTTCCAGGCTTTCTGGGTCTAGGAAGAGCGACCGGTACCCTTTCCGGGCTGGCAACGAGTTGCCGGAGTCTGTCCATTGGAGAGCTAAGGGCGCTGTTGCTACGGTGAAGGATCAAGGCCAATGCGGGAGTTGCTGGGCGTTCTCAACAGTCGGCGCAGTGGAAGGAATAAACAAGATTGTGACTGGTGAGCTGATCTCTTTGTCTGAGCAAGAACTAGTGGATTGTGACAGATCATACAACCAGGGATGCAATGGAGGTCTTATGGACTATGCCTTCCAATTCATCATCAACAATGGCGGCTTTGATGCTGAAGCAGATTACCCTTACCATGCTCGAGATGGTTCGTGTGATCCTAACGGGAAAAATGCCCGGGTTGTGTCCATTGATGGGTATGAAGATGTTTCAGAAAATGATGAGAAATCATTGAAGAAGGCTATGGCACATCAACCAGTTAGTGTTGCCATTGAAGCTGGTGGCAGGGAATTCCAACTTTACCAATCGGGTGTCTTCACTGGACGTTGTGGAACAGATCTAGATCACGGTGTGGTTGCTGTTGGTTATGGTACCGAAAATGGTGTAGACTACTGGATCGTGAGGAACTCATGGGGTCCCAGCTGGGTCGAGGCTGGTTACATCAGGTTGGAACGGAATGTGGCTAGCGCCAATACCGGCAAATGTGGTATTGCCATTGAGGCTTCATACCCTACCAAGAAGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000932 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004842 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005764 GO:0005773 GO:0005829 GO:0005911 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009888 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010494 GO:0010498 GO:0010605 GO:0016020 GO:0016049 GO:0016567 GO:0016740 GO:0016787 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021700 GO:0022622 GO:0030054 GO:0030154 GO:0030163 GO:0031146 GO:0031224 GO:0031225 GO:0031347 GO:0031348 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0035770 GO:0036211 GO:0036464 GO:0040007 GO:0042176 GO:0042177 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0048046 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051603 GO:0051704 GO:0051707 GO:0055044 GO:0060255 GO:0060560 GO:0065007 GO:0070011 GO:0070647 GO:0071695 GO:0071704 GO:0080090 GO:0080134 GO:0080147 GO:0090558 GO:0090627 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

366

Amino Acids

40.34

Weight (kDa)

5.89

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 58 - 114 5.8e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 146 - 362 2.9e-84 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36880
fragaria_vesca FvH4_2g05500
malus_domestica MD05G1086900.v1.1 MD12G1087000.v1.1
prunus_persica Prupe.8G131600_v2.0.a1
pyrus_communis pycom05g08620
rosa_chinensis RchiOBHm_Chr6g0255771
rosa_laevigata RLG00000014814
rosa_multiflora Rmu_sc0001163.1_g000010
rosa_roxburghii Rroxscaffold_7G00209680
rosa_rugosa Rorug05G0561400
rosa_samantha Rh6AG079300 Rh6BG072400 Rh6CG070600 Rh6DG067900
rosa_wichuraiana Rw6G006840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 136
Acc65I GGTACC 2 cut(s) 411, 941
AccB1I GGYRCC 2 cut(s) 411, 941
AccBSI CCGCTC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 957
AciI CCGC 4 cut(s) 84, 229, 501, 675
AclWI GGATC 3 cut(s) 495, 722, 974
AcoI YGGCCR 2 cut(s) 30, 81
AcsI RAATTY 1 cut(s) 860
AcuI CTGAAG 1 cut(s) 708
AdeI CACNNNGTG 1 cut(s) 922
AfaI GTAC 3 cut(s) 341, 413, 943
AfiI CCNNNNNNNGG 3 cut(s) 79, 421, 765
AflII CTTAAG 1 cut(s) 16
AflIII ACRYGT 1 cut(s) 93
AgeI ACCGGT 3 cut(s) 312, 342, 408
AgsI TTSAA 2 cut(s) 805, 845
AhlI ACTAGT 1 cut(s) 592
AjiI CACGTC 1 cut(s) 94
AjnI CCWGG 3 cut(s) 179, 380, 618
AluBI AGCT 4 cut(s) 461, 568, 848, 993
AluI AGCT 4 cut(s) 461, 568, 848, 993
Alw21I GWGCWC 1 cut(s) 276
AlwI GGATC 3 cut(s) 495, 722, 974
Ama87I CYCGRG 2 cut(s) 711, 747
AoxI GGCC 6 cut(s) 30, 36, 45, 54, 81, 493
ApoI RAATTY 1 cut(s) 860
AsiGI ACCGGT 3 cut(s) 312, 342, 408
Asp718I GGTACC 2 cut(s) 411, 941
AspLEI GCGC 3 cut(s) 470, 533, 1040
AspS9I GGNCC 5 cut(s) 55, 142, 147, 370, 986
AsuC2I CCSGG 4 cut(s) 374, 422, 748, 749
AsuHPI GGTGA 2 cut(s) 493, 575
AsuNHI GCTAGC 1 cut(s) 1034
AvaI CYCGRG 2 cut(s) 711, 747
AvaII GGWCC 4 cut(s) 142, 147, 370, 986
BalI TGGCCA 1 cut(s) 32
BanI GGYRCC 2 cut(s) 411, 941
BauI CACGAG 1 cut(s) 77
BbsI GAAGAC 1 cut(s) 877
Bbv12I GWGCWC 1 cut(s) 276
BccI CCATC 3 cut(s) 107, 710, 758
BceAI ACGGC 2 cut(s) 139, 211
BciT130I CCWGG 3 cut(s) 181, 382, 620
BcnI CCSGG 4 cut(s) 374, 422, 748, 749
BcuI ACTAGT 1 cut(s) 592
BfaI CTAG 4 cut(s) 396, 593, 911, 1035
BfmI CTRYAG 1 cut(s) 281
BfoI RGCGCY 2 cut(s) 471, 1041
BfrI CTTAAG 1 cut(s) 16
BglI GCCNNNNNGGC 3 cut(s) 44, 53, 1070
BglII AGATCT 2 cut(s) 20, 907
BisI GCNGC 2 cut(s) 84, 676
BlsI GCNGC 2 cut(s) 85, 677
Bme1390I CCNGG 7 cut(s) 181, 374, 382, 422, 620, 748, 749
Bme18I GGWCC 4 cut(s) 142, 147, 370, 986
BmeT110I CYCGRG 2 cut(s) 711, 747
BmgBI CACGTC 1 cut(s) 94
BmgT120I GGNCC 5 cut(s) 55, 142, 147, 370, 986
BmiI GGNNCC 5 cut(s) 379, 413, 943, 987, 988
BmrFI CCNGG 7 cut(s) 181, 374, 382, 422, 620, 748, 749
BmsI GCATC 2 cut(s) 614, 673
BmtI GCTAGC 1 cut(s) 1038
BpiI GAAGAC 1 cut(s) 877
BplI GAGNNNNNCTC 2 cut(s) 557, 589
Bpu10I CCTNAGC 1 cut(s) 462
BpuMI CCSGG 4 cut(s) 374, 422, 748, 749
BsaBI GATNNNNATC 1 cut(s) 912
BsaJI CCNNGG 6 cut(s) 33, 87, 363, 373, 619, 747
BsaWI WCCGGW 4 cut(s) 144, 312, 342, 408
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 421, 765
Bse118I RCCGGY 4 cut(s) 312, 342, 408, 1046
Bse1I ACTGG 4 cut(s) 565, 827, 895, 968
Bse3DI GCAATG 1 cut(s) 634
Bse8I GATNNNNATC 1 cut(s) 912
BseBI CCWGG 3 cut(s) 181, 382, 620
BseDI CCNNGG 6 cut(s) 33, 87, 363, 373, 619, 747
BseGI GGATG 2 cut(s) 63, 629
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 3 cut(s) 79, 421, 765
BseMI GCAATG 1 cut(s) 634
BseMII CTCAG 2 cut(s) 248, 573
BseNI ACTGG 4 cut(s) 565, 827, 895, 968
BseRI GAGGAG 3 cut(s) 50, 59, 350
BseYI CCCAGC 3 cut(s) 510, 989, 993
Bsh1285I CGRYCG 1 cut(s) 409
BshFI GGCC 6 cut(s) 32, 38, 47, 56, 83, 495
BshNI GGYRCC 2 cut(s) 411, 941
BshTI ACCGGT 3 cut(s) 312, 342, 408
BsiEI CGRYCG 1 cut(s) 409
BsiHKAI GWGCWC 1 cut(s) 276
BsiHKCI CYCGRG 2 cut(s) 711, 747
BslFI GGGAC 1 cut(s) 972
BslI CCNNNNNNNGG 3 cut(s) 79, 421, 765
BsmFI GGGAC 1 cut(s) 972
BsnI GGCC 6 cut(s) 32, 38, 47, 56, 83, 495
BsoBI CYCGRG 2 cut(s) 711, 747
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
Bsp19I CCATGG 2 cut(s) 33, 87
BspACI CCGC 4 cut(s) 84, 229, 501, 675
BspANI GGCC 6 cut(s) 32, 38, 47, 56, 83, 495
BspCNI CTCAG 2 cut(s) 249, 574
BspLI GGNNCC 5 cut(s) 379, 413, 943, 987, 988
BspOI GCTAGC 1 cut(s) 1038
BspPI GGATC 3 cut(s) 495, 722, 974
BspQI GCTCTTC 1 cut(s) 395
BspT107I GGYRCC 2 cut(s) 411, 941
BspTI CTTAAG 1 cut(s) 16
BsrBI CCGCTC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 634
BsrFI RCCGGY 4 cut(s) 312, 342, 408, 1046
BsrI ACTGG 4 cut(s) 565, 827, 895, 968
BssAI RCCGGY 4 cut(s) 312, 342, 408, 1046
BssECI CCNNGG 6 cut(s) 33, 87, 363, 373, 619, 747
BssMI GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
BssSI CACGAG 1 cut(s) 77
BssT1I CCWWGG 3 cut(s) 33, 87, 363
Bst2BI CACGAG 1 cut(s) 77
Bst2UI CCWGG 3 cut(s) 181, 382, 620
Bst4CI ACNGT 3 cut(s) 481, 526, 920
Bst6I CTCTTC 1 cut(s) 395
BstAFI CTTAAG 1 cut(s) 16
BstC8I GCNNGC 3 cut(s) 49, 427, 1036
BstDEI CTNAG 3 cut(s) 257, 462, 582
BstDSI CCRYGG 2 cut(s) 33, 87
BstF5I GGATG 2 cut(s) 63, 629
BstH2I RGCGCY 2 cut(s) 471, 1041
BstHHI GCGC 3 cut(s) 470, 533, 1040
BstKTI GATC 9 cut(s) 23, 325, 490, 573, 611, 730, 910, 916, 969
BstMBI GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
BstMCI CGRYCG 1 cut(s) 409
BstMWI GCNNNNNNNGC 7 cut(s) 44, 53, 190, 467, 507, 845, 1070
BstNI CCWGG 3 cut(s) 181, 382, 620
BstSCI CCNGG 7 cut(s) 179, 372, 380, 420, 618, 746, 747
BstSFI CTRYAG 1 cut(s) 281
BstV2I GAAGAC 1 cut(s) 877
BstX2I RGATCY 2 cut(s) 20, 907
BstYI RGATCY 2 cut(s) 20, 907
BsuRI GGCC 6 cut(s) 32, 38, 47, 56, 83, 495
BtgI CCRYGG 2 cut(s) 33, 87
BtrI CACGTC 1 cut(s) 94
BtsCI GGATG 2 cut(s) 63, 629
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 2 cut(s) 540, 888
Cac8I GCNNGC 3 cut(s) 49, 427, 1036
CfoI GCGC 3 cut(s) 470, 533, 1040
Cfr10I RCCGGY 4 cut(s) 312, 342, 408, 1046
Cfr13I GGNCC 5 cut(s) 55, 142, 147, 370, 986
Cfr9I CCCGGG 1 cut(s) 747
CpoI CGGWCCG 2 cut(s) 142, 147
Csp6I GTAC 3 cut(s) 340, 412, 942
CspAI ACCGGT 3 cut(s) 312, 342, 408
CspI CGGWCCG 2 cut(s) 142, 147
CviAII CATG 6 cut(s) 34, 52, 88, 127, 707, 981
CviQI GTAC 3 cut(s) 340, 412, 942
DdeI CTNAG 3 cut(s) 257, 462, 582
DpnI GATC 9 cut(s) 22, 324, 489, 572, 610, 729, 909, 915, 968
DpnII GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
DraIII CACNNNGTG 1 cut(s) 922
DrdI GACNNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 136
EaeI YGGCCR 2 cut(s) 30, 81
Eam1104I CTCTTC 1 cut(s) 395
EarI CTCTTC 1 cut(s) 395
Eco130I CCWWGG 3 cut(s) 33, 87, 363
Eco47I GGWCC 4 cut(s) 142, 147, 370, 986
Eco57I CTGAAG 1 cut(s) 708
Eco88I CYCGRG 2 cut(s) 711, 747
EcoO109I RGGNCCY 1 cut(s) 986
EcoRI GAATTC 1 cut(s) 860
EcoRII CCWGG 3 cut(s) 179, 380, 618
EcoT14I CCWWGG 3 cut(s) 33, 87, 363
ErhI CCWWGG 3 cut(s) 33, 87, 363
FaeI CATG 6 cut(s) 37, 55, 91, 130, 710, 984
FaqI GGGAC 1 cut(s) 972
FatI CATG 6 cut(s) 33, 51, 87, 126, 706, 980
FauI CCCGC 1 cut(s) 494
FblI GTMKAC 1 cut(s) 957
Fnu4HI GCNGC 2 cut(s) 84, 676
FokI GGATG 2 cut(s) 50, 636
Fsp4HI GCNGC 2 cut(s) 84, 676
FspBI CTAG 4 cut(s) 396, 593, 911, 1035
GlaI GCGC 3 cut(s) 469, 532, 1039
GluI GCNGC 2 cut(s) 84, 676
GsaI CCCAGC 3 cut(s) 514, 993, 997
HaeII RGCGCY 2 cut(s) 471, 1041
HaeIII GGCC 6 cut(s) 32, 38, 47, 56, 83, 495
HhaI GCGC 3 cut(s) 470, 533, 1040
Hin1II CATG 6 cut(s) 37, 55, 91, 130, 710, 984
Hin6I GCGC 3 cut(s) 468, 531, 1038
HinP1I GCGC 3 cut(s) 468, 531, 1038
HinfI GANTC 3 cut(s) 176, 234, 443
HphI GGTGA 2 cut(s) 493, 575
Hpy166II GTNNAC 3 cut(s) 352, 370, 958
Hpy188I TCNGA 5 cut(s) 142, 258, 327, 583, 784
Hpy188III TCNNGA 4 cut(s) 238, 713, 911, 970
Hpy8I GTNNAC 3 cut(s) 352, 370, 958
Hpy99I CGWCG 2 cut(s) 142, 272
HpyAV CCTTC 6 cut(s) 217, 478, 533, 661, 802, 1084
HpyCH4III ACNGT 3 cut(s) 481, 526, 920
HpyCH4IV ACGT 2 cut(s) 93, 895
HpyCH4V TGCA 2 cut(s) 51, 627
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 53, 190, 467, 507, 845, 1070
HpyF3I CTNAG 3 cut(s) 257, 462, 582
HpySE526I ACGT 2 cut(s) 93, 895
Hsp92II CATG 6 cut(s) 37, 55, 91, 130, 710, 984
HspAI GCGC 3 cut(s) 468, 531, 1038
KflI GGGWCCC 1 cut(s) 986
KpnI GGTACC 2 cut(s) 415, 945
Kzo9I GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
LguI GCTCTTC 1 cut(s) 395
LmnI GCTCC 1 cut(s) 226
LweI GCATC 2 cut(s) 614, 673
MaeI CTAG 4 cut(s) 396, 593, 911, 1035
MaeII ACGT 2 cut(s) 93, 895
MaeIII GTNAC 3 cut(s) 556, 602, 1007
MalI GATC 9 cut(s) 22, 324, 489, 572, 610, 729, 909, 915, 968
MbiI CCGCTC 1 cut(s) 229
MboI GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
MboII GAAGA 5 cut(s) 15, 412, 785, 817, 877
MflI RGATCY 2 cut(s) 20, 907
MhlI GDGCHC 1 cut(s) 276
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 3 cut(s) 202, 656, 860
MluNI TGGCCA 1 cut(s) 32
MlyI GAGTC 2 cut(s) 185, 452
MmeI TCCRAC 3 cut(s) 120, 889, 999
Mox20I TGGCCA 1 cut(s) 32
MscI TGGCCA 1 cut(s) 32
MseI TTAA 2 cut(s) 17, 246
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 993
MspCI CTTAAG 1 cut(s) 16
MspR9I CCNGG 7 cut(s) 181, 374, 382, 422, 620, 748, 749
MvaI CCWGG 3 cut(s) 181, 382, 620
MwoI GCNNNNNNNGC 7 cut(s) 44, 53, 190, 467, 507, 845, 1070
NciI CCSGG 4 cut(s) 374, 422, 748, 749
NcoI CCATGG 2 cut(s) 33, 87
NdeII GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
NheI GCTAGC 1 cut(s) 1034
NlaIII CATG 6 cut(s) 37, 55, 91, 130, 710, 984
NlaIV GGNNCC 5 cut(s) 379, 413, 943, 987, 988
NmuCI GTSAC 2 cut(s) 556, 602
PaeR7I CTCGAG 1 cut(s) 711
PciSI GCTCTTC 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 1 cut(s) 234
PinAI ACCGGT 3 cut(s) 312, 342, 408
PkrI GCNGC 2 cut(s) 85, 677
PleI GAGTC 2 cut(s) 184, 451
PpsI GAGTC 2 cut(s) 184, 451
PpuMI RGGWCCY 1 cut(s) 986
Psp5II RGGWCCY 1 cut(s) 986
Psp6I CCWGG 3 cut(s) 179, 380, 618
PspFI CCCAGC 3 cut(s) 510, 989, 993
PspGI CCWGG 3 cut(s) 179, 380, 618
PspN4I GGNNCC 5 cut(s) 379, 413, 943, 987, 988
PspPI GGNCC 5 cut(s) 55, 142, 147, 370, 986
PspPPI RGGWCCY 1 cut(s) 986
PsuI RGATCY 2 cut(s) 20, 907
PvuII CAGCTG 1 cut(s) 993
RsaI GTAC 3 cut(s) 341, 413, 943
RsaNI GTAC 3 cut(s) 340, 412, 942
RseI CAYNNNNRTG 1 cut(s) 92
Rsr2I CGGWCCG 2 cut(s) 142, 147
RsrII CGGWCCG 2 cut(s) 142, 147
SapI GCTCTTC 1 cut(s) 395
SaqAI TTAA 2 cut(s) 17, 246
SatI GCNGC 2 cut(s) 84, 676
Sau3AI GATC 9 cut(s) 20, 322, 487, 570, 608, 727, 907, 913, 966
Sau96I GGNCC 5 cut(s) 55, 142, 147, 370, 986
SchI GAGTC 2 cut(s) 185, 452
ScrFI CCNGG 7 cut(s) 181, 374, 382, 422, 620, 748, 749
SduI GDGCHC 1 cut(s) 276
SfaNI GCATC 2 cut(s) 614, 673
SfcI CTRYAG 1 cut(s) 281
SfiI GGCCNNNNNGGCC 2 cut(s) 44, 53
Sfr274I CTCGAG 1 cut(s) 711
SinI GGWCC 4 cut(s) 142, 147, 370, 986
SlaI CTCGAG 1 cut(s) 711
SmaI CCCGGG 1 cut(s) 749
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 2 cut(s) 16, 711
SmoI CTYRAG 2 cut(s) 16, 711
SpeI ACTAGT 1 cut(s) 592
Sse9I AATT 3 cut(s) 202, 656, 860
SsiI CCGC 4 cut(s) 84, 229, 501, 675
SspMI CTAG 4 cut(s) 396, 593, 911, 1035
StyD4I CCNGG 7 cut(s) 179, 372, 380, 420, 618, 746, 747
StyI CCWWGG 3 cut(s) 33, 87, 363
TaaI ACNGT 3 cut(s) 481, 526, 920
TaiI ACGT 2 cut(s) 96, 898
TaqI TCGA 5 cut(s) 105, 237, 267, 712, 999
TaqII GACCGA 1 cut(s) 164
TasI AATT 3 cut(s) 202, 656, 860
TauI GCSGC 2 cut(s) 86, 678
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 2 cut(s) 17, 246
Tru9I TTAA 2 cut(s) 17, 246
TscAI CASTG 2 cut(s) 540, 895
TseFI GTSAC 2 cut(s) 556, 602
Tsp45I GTSAC 2 cut(s) 556, 602
TspDTI ATGAA 5 cut(s) 17, 253, 649, 786, 1065
TspGWI ACGGA 1 cut(s) 1039
TspMI CCCGGG 1 cut(s) 747
TspRI CASTG 2 cut(s) 540, 895
Vha464I CTTAAG 1 cut(s) 16
VpaK11BI GGWCC 4 cut(s) 142, 147, 370, 986
XapI RAATTY 1 cut(s) 860
XbaI TCTAGA 1 cut(s) 910
XcmI CCANNNNNNNNNTGG 3 cut(s) 40, 626, 713
XhoI CTCGAG 1 cut(s) 711
XmaI CCCGGG 1 cut(s) 747
XmiI GTMKAC 1 cut(s) 957
XspI CTAG 4 cut(s) 396, 593, 911, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.