MD05G1096600.v1.1

Transcriptional regulator of RNA polII, SAGA, subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004824
Physical Location & Seq
Reverse (-)
20285089 .. 20287247
2159 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1096600.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGTGCCCAATCAGAGCTATTCTCGTATCGACACTTTGGAGCTGAAAGCTCTAATTATCCAAAAGATTGGGCATCAGAGAGCTGGCAAATACTTTGATCTGATGAGAAGATTGTTTAGTTCGAAGATTAGTAAGTGCGAATTTGACAAGTTTTGCAATCGGATTATTGGGAGGGAAAATGCCAATCTTCATAACCTGCTTATTAAGTCAATCCTCAAGAATGCCTGCCTCGCGAAAGTTCCGCCCTTGACCAGTGTTCGGAAAAAGGGAAGTACCCGTAATGTTAAAGTAGCAAATGGTTATCAGAAGGATGGTCTTCACTCGATTAATGCAGATGCATTTTCTCCATCCCCTCACAATATCCTGTCTCCAGTTAATCGAGAACGCAAGTTTCAGGACCGCCCAAGTCCTTTGAGGCCAAATGGAAAGTCCCAACATTTTTCCAAAGCACAAGAGCAGCAAAGTGCTACTGAATTGCTTTCTCTTGGAAGCAGGCCGCCTGTTGAAGTTGCCTCTGTAGAAGATGGAGAAGAGGTCGAACAGGATGCAGGAAGCCCTGCTATTCAAAGTAGAAGCCCAGTAACAGCTCCTCTCGGAATATATTTGGGTGGATCACGTAAAGCTCTTCCTAATGTATCAGTATGTAGCACTTACCACCCTTTGACCTGTCAAAACAGTGGTGAGCTACCTGATGCCAGATCATTAAGAAGTCGTTTGGAGCGGAAGTTGGAGAAGGAGGGTATTACTGTCGCTGTAGACTGTGCTAACCTCTTGAATAATGGATTGGATGCTTATCTGAAGAGGTTAATGGAACCTTGTATTCGGCTAGCCGGGACAAGACATGGCAGTGATCACTTAAAACAACTAGGTGGTTCATATCCGTACAACAGTGGTTCAAATGGAATGTTGATTGGGAGACATATGCAAAGAGAAACAAAATCTACTTATGCAACCATGTCAGATTTTTCTGCATCAGTGGAGCTAAATCCCAAAATGCTTGGTGTAGATTGGCCCATACAGCTCGAGAAGATTGTCTTGCGTGCATCTGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAGA-Tad1

Protein Analysis

352

Amino Acids

38.83

Weight (kDa)

9.48

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAGA-Tad1 PF12767 5 - 276 5.4e-59 Transcriptional regulator of RNA polII, SAGA, subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 4
AccBSI CCGCTC 1 cut(s) 721
AccI GTMKAC 1 cut(s) 756
AccII CGCG 1 cut(s) 233
AciI CCGC 4 cut(s) 242, 400, 497, 721
AclWI GGATC 1 cut(s) 619
AcsI RAATTY 1 cut(s) 140
AcuI CTGAAG 1 cut(s) 818
AfaI GTAC 2 cut(s) 274, 884
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 4 cut(s) 506, 566, 775, 897
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
AluBI AGCT 9 cut(s) 18, 43, 50, 83, 587, 623, 685, 982, 1021
AluI AGCT 9 cut(s) 18, 43, 50, 83, 587, 623, 685, 982, 1021
Alw26I GTCTC 2 cut(s) 372, 910
AlwI GGATC 1 cut(s) 619
Ama87I CYCGRG 1 cut(s) 1022
AoxI GGCC 3 cut(s) 416, 494, 1010
ApeKI GCWGC 1 cut(s) 457
ApoI RAATTY 1 cut(s) 140
ArsI GACNNNNNNTTYG 2 cut(s) 413, 445
AseI ATTAAT 1 cut(s) 327
AspS9I GGNCC 2 cut(s) 397, 1011
AsuC2I CCSGG 1 cut(s) 832
AsuHPI GGTGA 1 cut(s) 692
AsuII TTCGAA 1 cut(s) 122
AsuNHI GCTAGC 1 cut(s) 826
AvaI CYCGRG 1 cut(s) 1022
AvaII GGWCC 1 cut(s) 397
BaeGI GKGCMC 1 cut(s) 9
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 308
BbvI GCAGC 1 cut(s) 469
BccI CCATC 3 cut(s) 305, 355, 518
BcgI CGANNNNNNTGC 2 cut(s) 527, 561
BclI TGATCA 1 cut(s) 850
BcnI CCSGG 1 cut(s) 832
BcoDI GTCTC 2 cut(s) 372, 910
BfaI CTAG 2 cut(s) 827, 866
BfmI CTRYAG 2 cut(s) 516, 753
BfuAI ACCTGC 1 cut(s) 204
BisI GCNGC 2 cut(s) 458, 497
BlsI GCNGC 2 cut(s) 459, 498
Bme1390I CCNGG 1 cut(s) 832
Bme18I GGWCC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 1022
BmgT120I GGNCC 2 cut(s) 397, 1011
BmiI GGNNCC 2 cut(s) 6, 813
BmrFI CCNGG 1 cut(s) 832
BmrI ACTGGG 1 cut(s) 572
BmsI GCATC 7 cut(s) 82, 325, 535, 682, 778, 980, 1052
BmtI GCTAGC 1 cut(s) 830
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 1 cut(s) 308
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 354
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 832
BsaAI YACGTR 1 cut(s) 617
BsaBI GATNNNNATC 1 cut(s) 792
BsaXI ACNNNNNCTCC 4 cut(s) 519, 549, 710, 740
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse1I ACTGG 3 cut(s) 252, 371, 578
Bse8I GATNNNNATC 1 cut(s) 792
BseGI GGATG 4 cut(s) 316, 347, 550, 793
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 1 cut(s) 258
BseNI ACTGG 3 cut(s) 252, 371, 578
BseRI GAGGAG 1 cut(s) 579
BseSI GKGCMC 1 cut(s) 9
BseXI GCAGC 1 cut(s) 469
Bsh1236I CGCG 1 cut(s) 233
BshFI GGCC 3 cut(s) 418, 496, 1012
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 1 cut(s) 1022
BsiSI CCGG 1 cut(s) 831
BslFI GGGAC 2 cut(s) 415, 847
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 2 cut(s) 372, 910
BsmFI GGGAC 2 cut(s) 415, 847
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 3 cut(s) 418, 496, 1012
BsoBI CYCGRG 1 cut(s) 1022
Bsp119I TTCGAA 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 4 cut(s) 97, 611, 698, 850
Bsp68I TCGCGA 1 cut(s) 233
BspACI CCGC 4 cut(s) 242, 400, 497, 721
BspANI GGCC 3 cut(s) 418, 496, 1012
BspFNI CGCG 1 cut(s) 233
BspLI GGNNCC 2 cut(s) 6, 813
BspMI ACCTGC 1 cut(s) 204
BspOI GCTAGC 1 cut(s) 830
BspPI GGATC 1 cut(s) 619
BspQI GCTCTTC 1 cut(s) 630
BspT104I TTCGAA 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 4
BsrBI CCGCTC 1 cut(s) 721
BsrI ACTGG 3 cut(s) 252, 371, 578
BssMI GATC 4 cut(s) 97, 611, 698, 850
Bst4CI ACNGT 4 cut(s) 677, 748, 761, 890
Bst6I CTCTTC 4 cut(s) 525, 630, 794, 1044
BstBAI YACGTR 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 122
BstC8I GCNNGC 5 cut(s) 85, 226, 494, 828, 1041
BstF5I GGATG 4 cut(s) 316, 347, 550, 793
BstFNI CGCG 1 cut(s) 233
BstKTI GATC 4 cut(s) 100, 614, 701, 853
BstMAI GTCTC 2 cut(s) 372, 910
BstMBI GATC 4 cut(s) 97, 611, 698, 850
BstMWI GCNNNNNNNGC 2 cut(s) 230, 1018
BstSCI CCNGG 1 cut(s) 830
BstSFI CTRYAG 2 cut(s) 516, 753
BstSLI GKGCMC 1 cut(s) 9
BstUI CGCG 1 cut(s) 233
BstV1I GCAGC 1 cut(s) 469
BstV2I GAAGAC 1 cut(s) 308
BstXI CCANNNNNNTGG 1 cut(s) 68
BsuRI GGCC 3 cut(s) 418, 496, 1012
BtsCI GGATG 4 cut(s) 316, 347, 550, 793
BtsI GCAGTG 1 cut(s) 853
BtsIMutI CAGTG 5 cut(s) 259, 682, 853, 895, 981
BtuMI TCGCGA 1 cut(s) 233
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 5 cut(s) 85, 226, 494, 828, 1041
Cfr13I GGNCC 2 cut(s) 397, 1011
Csp6I GTAC 2 cut(s) 273, 883
CviAII CATG 2 cut(s) 842, 955
CviQI GTAC 2 cut(s) 273, 883
DpnI GATC 4 cut(s) 99, 613, 700, 852
DpnII GATC 4 cut(s) 97, 611, 698, 850
Eam1104I CTCTTC 4 cut(s) 525, 630, 794, 1044
EarI CTCTTC 4 cut(s) 525, 630, 794, 1044
EciI GGCGGA 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 1022
EcoT22I ATGCAT 1 cut(s) 340
FaeI CATG 2 cut(s) 845, 958
FalI AAGNNNNNCTT 2 cut(s) 1019, 1051
FaqI GGGAC 2 cut(s) 415, 847
FatI CATG 2 cut(s) 841, 954
FauNDI CATATG 1 cut(s) 921
FbaI TGATCA 1 cut(s) 850
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 2 cut(s) 458, 497
FokI GGATG 4 cut(s) 323, 334, 557, 800
Fsp4HI GCNGC 2 cut(s) 458, 497
FspBI CTAG 2 cut(s) 827, 866
GluI GCNGC 2 cut(s) 458, 497
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 3 cut(s) 418, 496, 1012
HapII CCGG 1 cut(s) 831
Hin1II CATG 2 cut(s) 845, 958
HpaII CCGG 1 cut(s) 831
HphI GGTGA 1 cut(s) 692
Hpy166II GTNNAC 1 cut(s) 757
Hpy188III TCNNGA 6 cut(s) 217, 232, 380, 395, 772, 1024
Hpy8I GTNNAC 1 cut(s) 757
HpyAV CCTTC 2 cut(s) 301, 727
HpyCH4III ACNGT 4 cut(s) 677, 748, 761, 890
HpyCH4IV ACGT 1 cut(s) 616
HpyCH4V TGCA 8 cut(s) 156, 332, 338, 548, 925, 950, 971, 1043
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 1018
HpySE526I ACGT 1 cut(s) 616
Hsp92II CATG 2 cut(s) 845, 958
Ksp22I TGATCA 1 cut(s) 850
Kzo9I GATC 4 cut(s) 97, 611, 698, 850
LguI GCTCTTC 1 cut(s) 630
LmnI GCTCC 4 cut(s) 40, 592, 718, 979
Lsp1109I GCAGC 1 cut(s) 469
LweI GCATC 7 cut(s) 82, 325, 535, 682, 778, 980, 1052
MaeI CTAG 2 cut(s) 827, 866
MaeII ACGT 1 cut(s) 616
MaeIII GTNAC 1 cut(s) 580
MalI GATC 4 cut(s) 99, 613, 700, 852
MbiI CCGCTC 1 cut(s) 721
MboI GATC 4 cut(s) 97, 611, 698, 850
MboII GAAGA 9 cut(s) 120, 136, 179, 308, 533, 542, 617, 811, 1039
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 3 cut(s) 54, 140, 473
MmeI TCCRAC 1 cut(s) 708
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 7 cut(s) 204, 285, 327, 375, 704, 806, 857
MslI CAYNNNNRTG 1 cut(s) 846
MspI CCGG 1 cut(s) 831
MspR9I CCNGG 1 cut(s) 832
Mva1269I GAATGC 1 cut(s) 226
MvnI CGCG 1 cut(s) 233
MwoI GCNNNNNNNGC 2 cut(s) 230, 1018
NciI CCSGG 1 cut(s) 832
NdeI CATATG 1 cut(s) 921
NdeII GATC 4 cut(s) 97, 611, 698, 850
NheI GCTAGC 1 cut(s) 826
NlaIII CATG 2 cut(s) 845, 958
NlaIV GGNNCC 2 cut(s) 6, 813
NruI TCGCGA 1 cut(s) 233
NsiI ATGCAT 1 cut(s) 340
NspV TTCGAA 1 cut(s) 122
PaeR7I CTCGAG 1 cut(s) 1022
PciSI GCTCTTC 1 cut(s) 630
PctI GAATGC 1 cut(s) 226
PkrI GCNGC 2 cut(s) 459, 498
Ppu21I YACGTR 1 cut(s) 617
PshBI ATTAAT 1 cut(s) 327
PspN4I GGNNCC 2 cut(s) 6, 813
PspPI GGNCC 2 cut(s) 397, 1011
RruI TCGCGA 1 cut(s) 233
RsaI GTAC 2 cut(s) 274, 884
RsaNI GTAC 2 cut(s) 273, 883
RseI CAYNNNNRTG 1 cut(s) 846
SapI GCTCTTC 1 cut(s) 630
SaqAI TTAA 7 cut(s) 204, 285, 327, 375, 704, 806, 857
SatI GCNGC 2 cut(s) 458, 497
Sau3AI GATC 4 cut(s) 97, 611, 698, 850
Sau96I GGNCC 2 cut(s) 397, 1011
ScrFI CCNGG 1 cut(s) 832
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 7 cut(s) 82, 325, 535, 682, 778, 980, 1052
SfcI CTRYAG 2 cut(s) 516, 753
Sfr274I CTCGAG 1 cut(s) 1022
SfuI TTCGAA 1 cut(s) 122
SinI GGWCC 1 cut(s) 397
SlaI CTCGAG 1 cut(s) 1022
SmiMI CAYNNNNRTG 1 cut(s) 846
SmlI CTYRAG 2 cut(s) 215, 1022
SmoI CTYRAG 2 cut(s) 215, 1022
Sse9I AATT 3 cut(s) 54, 140, 473
SsiI CCGC 4 cut(s) 242, 400, 497, 721
SspMI CTAG 2 cut(s) 827, 866
StyD4I CCNGG 1 cut(s) 830
TaaI ACNGT 4 cut(s) 677, 748, 761, 890
TaiI ACGT 1 cut(s) 619
TaqI TCGA 6 cut(s) 30, 122, 323, 379, 537, 1023
TasI AATT 3 cut(s) 54, 140, 473
TauI GCSGC 1 cut(s) 499
Tru1I TTAA 7 cut(s) 204, 285, 327, 375, 704, 806, 857
Tru9I TTAA 7 cut(s) 204, 285, 327, 375, 704, 806, 857
TscAI CASTG 5 cut(s) 259, 682, 853, 895, 981
TseI GCWGC 1 cut(s) 457
TspDTI ATGAA 2 cut(s) 179, 864
TspGWI ACGGA 1 cut(s) 870
TspRI CASTG 5 cut(s) 259, 682, 853, 895, 981
VpaK11BI GGWCC 1 cut(s) 397
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 1022
XmiI GTMKAC 1 cut(s) 756
XspI CTAG 2 cut(s) 827, 866
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.