MD10G1103000.v1.1

Transcriptional regulator of RNA polII, SAGA, subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004824
Physical Location & Seq
Reverse (-)
16743941 .. 16745570
1630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1103000.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGTGCTCAATCAGAGCTTTTCTCGTATCGACACTTTGGAGCTGAAAGCGCTAATTGTGCGAAAGATTGGGCATCAGAGAGCTGACAAATACTTTGATCTGATCAGAAGATTGTTTAGTTCAAAGATTAGCAAGTGCGAATTCGACAAGTTTTGCAATCGGATTATTGGGAGGGAAAATGTCAATCTTCATAACGGGCTTATTAAATCCATCCTCAAGAACGCCTGCCTTGCAAAACATCCGCCCTTGACCGGTGTTCGGAAAAGGGGATGCACCCTTAATGTTAAAGTAGCAAATGGTTATCAGAAGGATGCGCTTCACTCGATTAATGCAGATGCAGTTTCTCCATCCCCTGACAGGATCCTGTCTCCAGTTAATCGAGAACGCAAGTTTCAGGACCGCCCAAGTCCTTTGGGGCCAAATGGAAAGTCCCAACATTTTTCAAAAGCACAAGAGCAGCAAAGTGCTACTGAATTGCTTTCTTTTGGAAGCAGGCCGCCTGTTGAAGTTGCCTCTGTAGAAGATGGAGAAGAGGTTGAACAGGATGCAGGAAGCCCAGCTATTCAAAGTAGAAGCCCAGTAACTGCTCCACTCGGTATATATTTGGGTGGATCGCGTAAATCTCTTCCTAATGTATCAGTAAGTGGTGCTTACCACCCTGTGACCTGTCAAAACAGTGGTGAGCTACCTGATACGAGATCCTTAAGAAATCGTTTGGAGCGGAAGTTGGAGAAGGAGGGTATTAATGTCTCTATAGACTGTGTTAACCTCTTGAATAATGGATTGGATGCTTATCTGAAGAGGTTAATGGAACCTTGTATTCGGCTAGGCGGGACAAGGCATGGCAGTGATCACTTAAAACAAGTGAGTGGTTCATATCCGTACAACGATGGTTCAAATGGGATGTTGCGTGGAAGACATATGCAAAGAGAAACAAAATCTACTTATGCAACCATGTCAGATTTTTGTGCATCAGTGGAGCTAAATCCCCAAATGCTTGGCGTAGATTGGCCCATACAGCTCGAGAAGATTGTCTTGCGAGCATCTGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAGA-Tad1

Protein Analysis

352

Amino Acids

38.93

Weight (kDa)

9.27

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAGA-Tad1 PF12767 5 - 276 1.2e-59 Transcriptional regulator of RNA polII, SAGA, subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 721
AccII CGCG 1 cut(s) 616
AciI CCGC 5 cut(s) 242, 400, 497, 721, 831
AclWI GGATC 4 cut(s) 355, 368, 619, 693
AcsI RAATTY 1 cut(s) 140
AcuI CTGAAG 1 cut(s) 818
AdeI CACNNNGTG 1 cut(s) 661
AfaI GTAC 1 cut(s) 884
AfeI AGCGCT 1 cut(s) 51
AfiI CCNNNNNNNGG 3 cut(s) 251, 258, 357
AflII CTTAAG 1 cut(s) 703
AgeI ACCGGT 1 cut(s) 251
AgsI TTSAA 7 cut(s) 123, 444, 506, 539, 566, 775, 897
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
AluBI AGCT 7 cut(s) 18, 43, 83, 560, 685, 982, 1021
AluI AGCT 7 cut(s) 18, 43, 83, 560, 685, 982, 1021
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 2 cut(s) 372, 754
AlwI GGATC 4 cut(s) 355, 368, 619, 693
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 1022
Aor51HI AGCGCT 1 cut(s) 51
AoxI GGCC 3 cut(s) 416, 494, 1010
ApeKI GCWGC 1 cut(s) 457
ApoI RAATTY 1 cut(s) 140
ArsI GACNNNNNNTTYG 4 cut(s) 77, 109, 413, 445
AseI ATTAAT 2 cut(s) 327, 744
AsiGI ACCGGT 1 cut(s) 251
AspLEI GCGC 2 cut(s) 52, 316
AspS9I GGNCC 3 cut(s) 397, 416, 1011
AsuHPI GGTGA 1 cut(s) 692
AvaI CYCGRG 1 cut(s) 1022
AvaII GGWCC 1 cut(s) 397
BamHI GGATCC 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 922
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 1 cut(s) 469
BccI CCATC 4 cut(s) 218, 355, 518, 884
BclI TGATCA 2 cut(s) 102, 850
BcoDI GTCTC 2 cut(s) 372, 754
BfaI CTAG 1 cut(s) 827
BfmI CTRYAG 2 cut(s) 516, 753
BfoI RGCGCY 1 cut(s) 53
BfrI CTTAAG 1 cut(s) 703
BisI GCNGC 2 cut(s) 458, 497
BlsI GCNGC 2 cut(s) 459, 498
Bme18I GGWCC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 1022
BmgT120I GGNCC 3 cut(s) 397, 416, 1011
BmiI GGNNCC 3 cut(s) 362, 417, 813
BmrI ACTGGG 1 cut(s) 572
BmsI GCATC 8 cut(s) 82, 260, 301, 325, 535, 778, 980, 1052
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 1 cut(s) 922
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 200
BsaBI GATNNNNATC 1 cut(s) 792
BsaWI WCCGGW 1 cut(s) 251
BsaXI ACNNNNNCTCC 4 cut(s) 519, 549, 710, 740
Bsc4I CCNNNNNNNGG 3 cut(s) 251, 258, 357
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 2 cut(s) 371, 578
Bse8I GATNNNNATC 1 cut(s) 792
BseGI GGATG 8 cut(s) 210, 238, 275, 316, 347, 550, 793, 909
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 3 cut(s) 251, 258, 357
BseNI ACTGG 2 cut(s) 371, 578
BseXI GCAGC 1 cut(s) 469
BseYI CCCAGC 1 cut(s) 556
Bsh1236I CGCG 1 cut(s) 616
BshFI GGCC 3 cut(s) 418, 496, 1012
BshTI ACCGGT 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 1022
BsiSI CCGG 1 cut(s) 252
BslFI GGGAC 2 cut(s) 415, 847
BslI CCNNNNNNNGG 3 cut(s) 251, 258, 357
BsmAI GTCTC 2 cut(s) 372, 754
BsmFI GGGAC 2 cut(s) 415, 847
BsnI GGCC 3 cut(s) 418, 496, 1012
BsoBI CYCGRG 1 cut(s) 1022
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 6 cut(s) 97, 102, 360, 611, 698, 850
BspACI CCGC 5 cut(s) 242, 400, 497, 721, 831
BspANI GGCC 3 cut(s) 418, 496, 1012
BspFNI CGCG 1 cut(s) 616
BspLI GGNNCC 3 cut(s) 362, 417, 813
BspPI GGATC 4 cut(s) 355, 368, 619, 693
BspTI CTTAAG 1 cut(s) 703
BsrBI CCGCTC 1 cut(s) 721
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 2 cut(s) 371, 578
BssAI RCCGGY 1 cut(s) 251
BssMI GATC 6 cut(s) 97, 102, 360, 611, 698, 850
Bst4CI ACNGT 2 cut(s) 677, 761
Bst6I CTCTTC 4 cut(s) 525, 630, 794, 1044
BstAFI CTTAAG 1 cut(s) 703
BstC8I GCNNGC 3 cut(s) 226, 494, 1041
BstF5I GGATG 8 cut(s) 210, 238, 275, 316, 347, 550, 793, 909
BstFNI CGCG 1 cut(s) 616
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 2 cut(s) 52, 316
BstKTI GATC 6 cut(s) 100, 105, 363, 614, 701, 853
BstMAI GTCTC 2 cut(s) 372, 754
BstMBI GATC 6 cut(s) 97, 102, 360, 611, 698, 850
BstMWI GCNNNNNNNGC 4 cut(s) 49, 58, 230, 1018
BstSFI CTRYAG 2 cut(s) 516, 753
BstUI CGCG 1 cut(s) 616
BstV1I GCAGC 1 cut(s) 469
BstV2I GAAGAC 1 cut(s) 922
BstX2I RGATCY 2 cut(s) 360, 698
BstXI CCANNNNNNTGG 1 cut(s) 998
BstYI RGATCY 2 cut(s) 360, 698
BsuRI GGCC 3 cut(s) 418, 496, 1012
BtsCI GGATG 8 cut(s) 210, 238, 275, 316, 347, 550, 793, 909
BtsI GCAGTG 1 cut(s) 853
BtsIMutI CAGTG 3 cut(s) 682, 853, 981
Cac8I GCNNGC 3 cut(s) 226, 494, 1041
CaiI CAGNNNCTG 1 cut(s) 584
CfoI GCGC 2 cut(s) 52, 316
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 3 cut(s) 397, 416, 1011
Csp6I GTAC 1 cut(s) 883
CspAI ACCGGT 1 cut(s) 251
CviAII CATG 2 cut(s) 842, 955
CviQI GTAC 1 cut(s) 883
DpnI GATC 6 cut(s) 99, 104, 362, 613, 700, 852
DpnII GATC 6 cut(s) 97, 102, 360, 611, 698, 850
DraIII CACNNNGTG 1 cut(s) 661
Eam1104I CTCTTC 4 cut(s) 525, 630, 794, 1044
EarI CTCTTC 4 cut(s) 525, 630, 794, 1044
EciI GGCGGA 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 397
Eco47III AGCGCT 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 1022
EcoRI GAATTC 1 cut(s) 140
FaeI CATG 2 cut(s) 845, 958
FalI AAGNNNNNCTT 4 cut(s) 634, 666, 1019, 1051
FaqI GGGAC 2 cut(s) 415, 847
FatI CATG 2 cut(s) 841, 954
FauI CCCGC 1 cut(s) 824
FauNDI CATATG 1 cut(s) 921
FbaI TGATCA 2 cut(s) 102, 850
Fnu4HI GCNGC 2 cut(s) 458, 497
FokI GGATG 8 cut(s) 197, 225, 282, 323, 334, 557, 800, 916
Fsp4HI GCNGC 2 cut(s) 458, 497
FspBI CTAG 1 cut(s) 827
GlaI GCGC 2 cut(s) 51, 315
GluI GCNGC 2 cut(s) 458, 497
GsaI CCCAGC 1 cut(s) 560
GsuI CTGGAG 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 3 cut(s) 418, 496, 1012
HapII CCGG 1 cut(s) 252
HhaI GCGC 2 cut(s) 52, 316
Hin1II CATG 2 cut(s) 845, 958
Hin6I GCGC 2 cut(s) 50, 314
HinP1I GCGC 2 cut(s) 50, 314
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HpaI GTTAAC 1 cut(s) 766
HpaII CCGG 1 cut(s) 252
HphI GGTGA 1 cut(s) 692
Hpy166II GTNNAC 1 cut(s) 766
Hpy188III TCNNGA 5 cut(s) 217, 380, 395, 772, 1024
Hpy8I GTNNAC 1 cut(s) 766
HpyAV CCTTC 2 cut(s) 301, 727
HpyCH4III ACNGT 2 cut(s) 677, 761
HpyCH4V TGCA 9 cut(s) 156, 233, 273, 332, 338, 548, 925, 950, 971
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 58, 230, 1018
Hsp92II CATG 2 cut(s) 845, 958
HspAI GCGC 2 cut(s) 50, 314
Ksp22I TGATCA 2 cut(s) 102, 850
KspAI GTTAAC 1 cut(s) 766
Kzo9I GATC 6 cut(s) 97, 102, 360, 611, 698, 850
LmnI GCTCC 4 cut(s) 40, 592, 718, 979
Lsp1109I GCAGC 1 cut(s) 469
LweI GCATC 8 cut(s) 82, 260, 301, 325, 535, 778, 980, 1052
MaeI CTAG 1 cut(s) 827
MaeIII GTNAC 2 cut(s) 580, 661
MalI GATC 6 cut(s) 99, 104, 362, 613, 700, 852
MbiI CCGCTC 1 cut(s) 721
MboI GATC 6 cut(s) 97, 102, 360, 611, 698, 850
MboII GAAGA 8 cut(s) 120, 179, 533, 542, 617, 811, 927, 1039
MflI RGATCY 2 cut(s) 360, 698
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 3 cut(s) 54, 140, 473
MmeI TCCRAC 1 cut(s) 708
MnlI CCTC 7 cut(s) 165, 224, 523, 526, 730, 779, 795
MslI CAYNNNNRTG 1 cut(s) 846
MspCI CTTAAG 1 cut(s) 703
MspI CCGG 1 cut(s) 252
MvnI CGCG 1 cut(s) 616
MwoI GCNNNNNNNGC 4 cut(s) 49, 58, 230, 1018
NdeI CATATG 1 cut(s) 921
NdeII GATC 6 cut(s) 97, 102, 360, 611, 698, 850
NlaIII CATG 2 cut(s) 845, 958
NlaIV GGNNCC 3 cut(s) 362, 417, 813
NmuCI GTSAC 1 cut(s) 661
PaeR7I CTCGAG 1 cut(s) 1022
PinAI ACCGGT 1 cut(s) 251
PkrI GCNGC 2 cut(s) 459, 498
PshBI ATTAAT 2 cut(s) 327, 744
PspFI CCCAGC 1 cut(s) 556
PspN4I GGNNCC 3 cut(s) 362, 417, 813
PspPI GGNCC 3 cut(s) 397, 416, 1011
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 2 cut(s) 360, 698
RsaI GTAC 1 cut(s) 884
RsaNI GTAC 1 cut(s) 883
RseI CAYNNNNRTG 1 cut(s) 846
SatI GCNGC 2 cut(s) 458, 497
Sau3AI GATC 6 cut(s) 97, 102, 360, 611, 698, 850
Sau96I GGNCC 3 cut(s) 397, 416, 1011
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 8 cut(s) 82, 260, 301, 325, 535, 778, 980, 1052
SfcI CTRYAG 2 cut(s) 516, 753
Sfr274I CTCGAG 1 cut(s) 1022
SinI GGWCC 1 cut(s) 397
SlaI CTCGAG 1 cut(s) 1022
SmiMI CAYNNNNRTG 1 cut(s) 846
SmlI CTYRAG 3 cut(s) 215, 703, 1022
SmoI CTYRAG 3 cut(s) 215, 703, 1022
Sse9I AATT 3 cut(s) 54, 140, 473
SsiI CCGC 5 cut(s) 242, 400, 497, 721, 831
SspMI CTAG 1 cut(s) 827
TaaI ACNGT 2 cut(s) 677, 761
TaqI TCGA 5 cut(s) 30, 144, 323, 379, 1023
TasI AATT 3 cut(s) 54, 140, 473
TauI GCSGC 1 cut(s) 499
TscAI CASTG 3 cut(s) 682, 853, 981
TseFI GTSAC 1 cut(s) 661
TseI GCWGC 1 cut(s) 457
Tsp45I GTSAC 1 cut(s) 661
TspDTI ATGAA 2 cut(s) 179, 864
TspGWI ACGGA 1 cut(s) 870
TspRI CASTG 3 cut(s) 682, 853, 981
Vha464I CTTAAG 1 cut(s) 703
VpaK11BI GGWCC 1 cut(s) 397
VspI ATTAAT 2 cut(s) 327, 744
XapI RAATTY 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 1022
XspI CTAG 1 cut(s) 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.