MD05G1098200.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005585
Physical Location & Seq
Reverse (-)
20408134 .. 20411987
3854 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1098200.v1.1.491

Sequence Viewer

Length: 642 bp
ATGAAGTTTATGAAACTTGGATCGAAGCCGGATTCTTTTCAAACTGATGAAAATAATGTTAGGTATGTCGCAACTGATTTGGCAACAGACATCATTGTTAATGTTGGAGATTCAAAGTTTTACCTACATAAGTTTCCTCTTCTATCAAAGAGTGCCCGCTTACAGAAGTGGGTTGCAAACTCTAATGAATCAAACACTGGTGAAATTTACATTTCTGACATTCCTGGTGGCCCTGCTGCCTTTGAGATATGTGCGAAGTTTTGTTATGGCATGACTGTCACCCTCAATGCTTACAATGTTGTTGTGTCTCGCTGTGCTGCTGAGTATCTGGGGATGCATGAGTCTATCGAGAAAGGAAATCTCATATACAAGATTGATGTCTTCCTTAGCTCTAGCATTTTCCGGAGCTGGAAGGATTCCATTATTGTTCTTCAGACTACGAAGTCTCTTTTACCAATATCTGAGGAACTGAAGTTGGTCAGCCATGGCATTGACTCTATCGCTACTAAGGCCTGCATGGATATTTCAAAAGTTGACTGGTCCTATACCTATAACCGGAAGAAGATCCAAGAGGAAAATGGGAATGATCCAAATTGGAATGGTGTAAGAAATCGTCCAGTTCCAAAAGACTGCAAATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009958 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

214

Amino Acids

24.03

Weight (kDa)

8.18

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 28 - 118 2.2e-06 BTB/POZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 442
AccIII TCCGGA 1 cut(s) 402
AciI CCGC 1 cut(s) 157
AclWI GGATC 3 cut(s) 28, 559, 581
AcsI RAATTY 1 cut(s) 204
AcuI CTGAAG 2 cut(s) 416, 491
AfiI CCNNNNNNNGG 1 cut(s) 555
AgsI TTSAA 3 cut(s) 41, 114, 528
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 2 cut(s) 390, 408
AluI AGCT 2 cut(s) 390, 408
Alw26I GTCTC 2 cut(s) 312, 450
AlwI GGATC 3 cut(s) 28, 559, 581
Aor13HI TCCGGA 1 cut(s) 402
AoxI GGCC 2 cut(s) 229, 510
ApeKI GCWGC 2 cut(s) 236, 317
ApoI RAATTY 1 cut(s) 204
AspS9I GGNCC 2 cut(s) 230, 540
AsuHPI GGTGA 2 cut(s) 212, 271
AvaII GGWCC 1 cut(s) 540
BaeGI GKGCMC 1 cut(s) 157
BbsI GAAGAC 1 cut(s) 373
BbvI GCAGC 2 cut(s) 223, 304
BciT130I CCWGG 1 cut(s) 225
BcoDI GTCTC 2 cut(s) 312, 450
BfaI CTAG 1 cut(s) 393
BisI GCNGC 2 cut(s) 237, 318
BlsI GCNGC 2 cut(s) 238, 319
Bme1390I CCNGG 1 cut(s) 225
Bme18I GGWCC 1 cut(s) 540
BmgT120I GGNCC 2 cut(s) 230, 540
BmrFI CCNGG 1 cut(s) 225
BmsI GCATC 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 373
Bpu10I CCTNAGC 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 484
BsaWI WCCGGW 2 cut(s) 402, 555
Bsc4I CCNNNNNNNGG 1 cut(s) 555
Bse1I ACTGG 3 cut(s) 202, 542, 617
BseAI TCCGGA 1 cut(s) 402
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 484
BseGI GGATG 1 cut(s) 339
BseLI CCNNNNNNNGG 1 cut(s) 555
BseMII CTCAG 2 cut(s) 312, 453
BseNI ACTGG 3 cut(s) 202, 542, 617
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 2 cut(s) 223, 304
BshFI GGCC 2 cut(s) 231, 512
BsiSI CCGG 3 cut(s) 29, 403, 556
BslI CCNNNNNNNGG 1 cut(s) 555
BsmAI GTCTC 2 cut(s) 312, 450
BsnI GGCC 2 cut(s) 231, 512
Bsp1286I GDGCHC 1 cut(s) 157
Bsp13I TCCGGA 1 cut(s) 402
Bsp143I GATC 3 cut(s) 20, 564, 586
Bsp19I CCATGG 1 cut(s) 484
BspACI CCGC 1 cut(s) 157
BspANI GGCC 2 cut(s) 231, 512
BspCNI CTCAG 2 cut(s) 313, 454
BspEI TCCGGA 1 cut(s) 402
BspPI GGATC 3 cut(s) 28, 559, 581
BsrI ACTGG 3 cut(s) 202, 542, 617
BssECI CCNNGG 1 cut(s) 484
BssMI GATC 3 cut(s) 20, 564, 586
BssT1I CCWWGG 1 cut(s) 484
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 157, 514
BstDEI CTNAG 4 cut(s) 321, 386, 462, 507
BstDSI CCRYGG 1 cut(s) 484
BstF5I GGATG 1 cut(s) 339
BstKTI GATC 3 cut(s) 23, 567, 589
BstMAI GTCTC 2 cut(s) 312, 450
BstMBI GATC 3 cut(s) 20, 564, 586
BstMWI GCNNNNNNNGC 1 cut(s) 509
BstNI CCWGG 1 cut(s) 225
BstSCI CCNGG 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 223, 304
BstV2I GAAGAC 1 cut(s) 373
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
BsuRI GGCC 2 cut(s) 231, 512
BtgI CCRYGG 1 cut(s) 484
BtsCI GGATG 1 cut(s) 339
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 157, 514
Cfr13I GGNCC 2 cut(s) 230, 540
CviAII CATG 4 cut(s) 271, 338, 485, 517
CviJI RGCY 6 cut(s) 28, 231, 390, 408, 483, 512
CviKI_1 RGCY 6 cut(s) 28, 231, 390, 408, 483, 512
DdeI CTNAG 4 cut(s) 321, 386, 462, 507
DpnI GATC 3 cut(s) 22, 566, 588
DpnII GATC 3 cut(s) 20, 564, 586
DrdI GACNNNNNNGTC 1 cut(s) 442
DseDI GACNNNNNNGTC 1 cut(s) 442
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 484
Eco147I AGGCCT 1 cut(s) 512
Eco47I GGWCC 1 cut(s) 540
Eco57I CTGAAG 2 cut(s) 416, 491
EcoRII CCWGG 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 484
EcoT22I ATGCAT 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 484
FaeI CATG 4 cut(s) 274, 341, 488, 520
FatI CATG 4 cut(s) 270, 337, 484, 516
FauI CCCGC 1 cut(s) 164
Fnu4HI GCNGC 2 cut(s) 237, 318
FokI GGATG 1 cut(s) 346
Fsp4HI GCNGC 2 cut(s) 237, 318
FspBI CTAG 1 cut(s) 393
GluI GCNGC 2 cut(s) 237, 318
HaeIII GGCC 2 cut(s) 231, 512
HapII CCGG 3 cut(s) 29, 403, 556
Hin1II CATG 4 cut(s) 274, 341, 488, 520
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 6 cut(s) 32, 110, 188, 341, 416, 494
HpaII CCGG 3 cut(s) 29, 403, 556
HphI GGTGA 2 cut(s) 212, 271
Hpy166II GTNNAC 1 cut(s) 535
Hpy188I TCNGA 3 cut(s) 217, 435, 463
Hpy188III TCNNGA 2 cut(s) 349, 403
Hpy8I GTNNAC 1 cut(s) 535
HpyAV CCTTC 1 cut(s) 406
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4V TGCA 4 cut(s) 176, 337, 516, 633
HpyF10VI GCNNNNNNNGC 1 cut(s) 509
HpyF3I CTNAG 4 cut(s) 321, 386, 462, 507
Hsp92II CATG 4 cut(s) 274, 341, 488, 520
Kpn2I TCCGGA 1 cut(s) 402
Kzo9I GATC 3 cut(s) 20, 564, 586
LmnI GCTCC 1 cut(s) 405
Lsp1109I GCAGC 2 cut(s) 223, 304
LweI GCATC 1 cut(s) 324
MaeI CTAG 1 cut(s) 393
MaeIII GTNAC 1 cut(s) 277
MalI GATC 3 cut(s) 22, 566, 588
MboI GATC 3 cut(s) 20, 564, 586
MboII GAAGA 5 cut(s) 131, 373, 422, 571, 574
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 157
MluCI AATT 2 cut(s) 204, 592
MlyI GAGTC 2 cut(s) 350, 488
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 4 cut(s) 147, 293, 457, 565
Mph1103I ATGCAT 1 cut(s) 339
MroI TCCGGA 1 cut(s) 402
MseI TTAA 2 cut(s) 99, 640
MspI CCGG 3 cut(s) 29, 403, 556
MspR9I CCNGG 1 cut(s) 225
MvaI CCWGG 1 cut(s) 225
MwoI GCNNNNNNNGC 1 cut(s) 509
NcoI CCATGG 1 cut(s) 484
NdeII GATC 3 cut(s) 20, 564, 586
NlaIII CATG 4 cut(s) 274, 341, 488, 520
NmuCI GTSAC 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 339
PceI AGGCCT 1 cut(s) 512
PfeI GAWTC 4 cut(s) 32, 110, 188, 416
PkrI GCNGC 2 cut(s) 238, 319
PleI GAGTC 2 cut(s) 349, 488
PpsI GAGTC 2 cut(s) 349, 488
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspPI GGNCC 2 cut(s) 230, 540
PsuI RGATCY 1 cut(s) 564
SaqAI TTAA 2 cut(s) 99, 640
SatI GCNGC 2 cut(s) 237, 318
Sau3AI GATC 3 cut(s) 20, 564, 586
Sau96I GGNCC 2 cut(s) 230, 540
SchI GAGTC 2 cut(s) 350, 488
ScrFI CCNGG 1 cut(s) 225
SduI GDGCHC 1 cut(s) 157
SetI ASST 5 cut(s) 65, 126, 392, 410, 551
SfaNI GCATC 1 cut(s) 324
SinI GGWCC 1 cut(s) 540
Sse9I AATT 2 cut(s) 204, 592
SseBI AGGCCT 1 cut(s) 512
SsiI CCGC 1 cut(s) 157
SspI AATATT 1 cut(s) 638
SspMI CTAG 1 cut(s) 393
StuI AGGCCT 1 cut(s) 512
StyD4I CCNGG 1 cut(s) 223
StyI CCWWGG 1 cut(s) 484
TaaI ACNGT 1 cut(s) 277
TaqI TCGA 2 cut(s) 23, 348
TasI AATT 2 cut(s) 204, 592
TfiI GAWTC 4 cut(s) 32, 110, 188, 416
Tru1I TTAA 2 cut(s) 99, 640
Tru9I TTAA 2 cut(s) 99, 640
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 2 cut(s) 236, 317
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 4 cut(s) 17, 26, 63, 201
TspRI CASTG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 540
XapI RAATTY 1 cut(s) 204
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XspI CTAG 1 cut(s) 393
Zsp2I ATGCAT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.