RchiOBHm_Chr6g0253481

NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005585
Physical Location & Seq
Reverse (-)
8575435 .. 8581304
5870 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22730

Sequence Viewer

Length: 1866 bp
ATGAAGTTTATGAAACTTGGATCAAAGCCGGATTCCTTTCAGACTGATGGCAATAATGTTAGGTATGTGGCAGCTGATTTGGAAACAGATATCATCGTTAATGTTGGAGTTTCAAAGTTTTATCTACACAAGTTTCCTCTTCTATCAAAGAGTGCCCGCTTACAGAAATTAGCAAACACCAATGAGTCGAAACCTTTGGAAATTCACATTTCAGACATTCCTGGTGGCCCTGCTGCATTTGAGATATGTGCCAAGTTCTGTTATGGCATGACTGTCACCCTGAATGCTTACAATGTTGTTGTGTGTCGATGTGCGGCTGAGTATATGGGAATGCACGAGTCTATTGAGAAAGGAAATCTCATATACAAGATTGATGTGTTTCTTAACTCTAGCATTTTCCGCAGCTGGAAGGATTCAATCATTGTTCTTCAGACTACAAAGTCTCTTTTGCCGATATCTGAGGACCTGAAGTTGGTCAGCCAGTGCATTGATTCGATAGCTACCAAGGCCTGTGTCGATGTTTCAAAAGTTGACTGGTCCTATACCTATAACCGGAAGAAACTTGCAGAGGAAAATGGGAACGATCCAAATTGGAATGGTGTCAGAAACCGTCAAGTTCCAAAGGATTGGTGGGTGGAGGAATTATGTGAGCTTGAGATTGATTTGTTTAAGCGTGTTCTTATAAGTATTAAAACCAAAGCAGTACTTTCTGGTGAAGTGATCGGAGAAGCCTTGAAAGCTTATGCTTACAGAAGGTTGCCAGGTTTCAGCAAAGGTATGATCCAGGGTGGAGATATGGCAAAGCATCGATCAACAGTGGATGCAATTGTCTGGCTGTTGCCTGCAGAGAAAGGCAGCATCTCTTGTAGTTTCTTGCTCAAATTGTTAAAAGCAGCTATATATGTGAACTCTGGAGAAACGACCAAGGATGAGATGGTAAAGAGAATAGGACAGCAACTGGAGGAGGCTTCAGTAAATGATCTTTTGATACGAGCAGCAGAAGGAGAAGCTACTATATATGATGTTAGTGCAGTTAAAAAAATAGTAGAAGAGTTTCTAAAGAAAGATCAGACTGCAGAGATTCAATCATTAGGAGAGGGCCATGAGATTCAGGAGGAGAGGAGGCCAGGGATCTTGTCAGATGCTTCTAAGTTGATGGTGGCAAAACTGATAGATGGGTATCTTGCTGAAATTTCAAAGGATCCGAACCTACCCTTGTCCAAGTTTGTCGATCTTGCAGAAATGGTGTCAGGTTTCTCTCGCCCTGCTCATGATGGGCTTTACCGAGCCATTGACATGTTTCTCAAGGAGCACCCTGGGATTAGTAAGAGTGAGAGAAAGAGGATCTGCAAGCTGATGGACTGCAAAAAGCTGTCAGTTGATGCCTGCATGCACGCTGTGCAAAATGAGAGACTCCCGTTGCGCGTAGTTGTGCAGGTACTCTTTTTTGAGCAAGTCAGGGCTGCTGCTTCATCAGGCAGTAGCACTCCCGACTTACCTAAAGGCATGAAGGATTTAAACAGTGGTTCTCATGGGAGCTCAAGGTCTGCAACAACTAACACAGAGGAGGATTGGGATGTAGTTGCGTCTGCTGAGGAGCTTAGGGCCCTGAAAGCAGAGTTAGCTGCCTTAAGATTGGCCAGTGGTGAGAGGAATGGAGATGGTAAAAGCAATGTTGACAAGGCTGCAGTTAGTAAAATGAGAAGGTTACTCGATTCAAAGAAGGTCTTTGCAAAGCTCTTTTCAAGCAAAGGGGGACAAGGCGAGAATAGTGGCTCAGATTCATCCGAGAGTCTTGGTTCTGCAAATCCTGAAGAGGCTGCTAAGTCCACACCTTCCAGAAATCGGAGGCATTCGGTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009958 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

621

Amino Acids

68.48

Weight (kDa)

8.29

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 209 - 463 4.9e-90 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 683
AasI GACNNNNNNGTC 1 cut(s) 439
Acc36I ACCTGC 1 cut(s) 1426
AccII CGCG 1 cut(s) 1425
AciI CCGC 3 cut(s) 157, 314, 400
AclWI GGATC 7 cut(s) 28, 578, 775, 1139, 1196, 1209, 1352
AcoI YGGCCR 1 cut(s) 1638
AcsI RAATTY 2 cut(s) 201, 1191
AcuI CTGAAG 4 cut(s) 413, 488, 954, 1833
AdeI CACNNNGTG 1 cut(s) 1399
AfaI GTAC 2 cut(s) 705, 1440
AfiI CCNNNNNNNGG 3 cut(s) 472, 552, 1845
AflII CTTAAG 1 cut(s) 1630
AflIII ACRYGT 1 cut(s) 1296
AgsI TTSAA 8 cut(s) 114, 417, 525, 736, 1085, 1197, 1719, 1746
AjnI CCWGG 5 cut(s) 220, 760, 783, 1126, 1315
Alw21I GWGCWC 2 cut(s) 1314, 1541
Alw26I GTCTC 2 cut(s) 447, 1405
AlwI GGATC 7 cut(s) 28, 578, 775, 1139, 1196, 1209, 1352
AlwNI CAGNNNCTG 1 cut(s) 958
AoxI GGCC 6 cut(s) 226, 507, 1099, 1124, 1605, 1638
ApaI GGGCCC 1 cut(s) 1609
ApoI RAATTY 2 cut(s) 201, 1191
Asp700I GAANNNNTTC 1 cut(s) 1053
AspLEI GCGC 1 cut(s) 1425
AspS9I GGNCC 6 cut(s) 227, 463, 537, 1099, 1605, 1606
AsuHPI GGTGA 3 cut(s) 268, 725, 1658
AvaII GGWCC 2 cut(s) 463, 537
BaeGI GKGCMC 2 cut(s) 157, 1609
BalI TGGCCA 1 cut(s) 1640
BamHI GGATCC 1 cut(s) 1201
BanII GRGCYC 2 cut(s) 1541, 1609
BauI CACGAG 1 cut(s) 335
Bbv12I GWGCWC 2 cut(s) 1314, 1541
BbvCI CCTCAGC 1 cut(s) 1593
BccI CCATC 7 cut(s) 41, 928, 1150, 1169, 1268, 1351, 1655
BciT130I CCWGG 5 cut(s) 222, 762, 785, 1128, 1317
BcoDI GTCTC 2 cut(s) 447, 1405
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 3 cut(s) 843, 1074, 1686
BfrI CTTAAG 1 cut(s) 1630
BfuAI ACCTGC 1 cut(s) 1426
BmcAI AGTACT 1 cut(s) 705
Bme1390I CCNGG 5 cut(s) 222, 762, 785, 1128, 1317
Bme18I GGWCC 2 cut(s) 463, 537
BmgT120I GGNCC 6 cut(s) 227, 463, 537, 1099, 1605, 1606
BmiI GGNNCC 2 cut(s) 1203, 1607
BmrFI CCNGG 5 cut(s) 222, 762, 785, 1128, 1317
BmsI GCATC 5 cut(s) 811, 814, 867, 1132, 1372
BpmI CTGGAG 2 cut(s) 933, 980
Bpu10I CCTNAGC 2 cut(s) 1593, 1601
BpuEI CTTGAG 3 cut(s) 674, 1289, 1525
Bsa29I ATCGAT 1 cut(s) 808
BsaBI GATNNNNATC 1 cut(s) 1179
BsaJI CCNNGG 6 cut(s) 504, 784, 924, 1127, 1315, 1316
BsaWI WCCGGW 1 cut(s) 552
Bsc4I CCNNNNNNNGG 3 cut(s) 472, 552, 1845
Bse1I ACTGG 4 cut(s) 481, 539, 963, 1641
Bse3DI GCAATG 1 cut(s) 1678
Bse8I GATNNNNATC 1 cut(s) 1179
BseBI CCWGG 5 cut(s) 222, 762, 785, 1128, 1317
BseCI ATCGAT 1 cut(s) 808
BseDI CCNNGG 6 cut(s) 504, 784, 924, 1127, 1315, 1316
BseGI GGATG 4 cut(s) 826, 934, 1582, 1784
BseJI GATNNNNATC 1 cut(s) 1179
BseLI CCNNNNNNNGG 3 cut(s) 472, 552, 1845
BseMI GCAATG 1 cut(s) 1678
BseMII CTCAG 4 cut(s) 309, 450, 1584, 1791
BseNI ACTGG 4 cut(s) 481, 539, 963, 1641
BseRI GAGGAG 5 cut(s) 977, 1130, 1135, 1580, 1610
BseSI GKGCMC 2 cut(s) 157, 1609
BsgI GTGCAG 2 cut(s) 1050, 1454
Bsh1236I CGCG 1 cut(s) 1425
BshFI GGCC 6 cut(s) 228, 509, 1101, 1126, 1607, 1640
BshVI ATCGAT 1 cut(s) 808
BsiHKAI GWGCWC 2 cut(s) 1314, 1541
BsiSI CCGG 2 cut(s) 29, 553
BslFI GGGAC 1 cut(s) 1770
BslI CCNNNNNNNGG 3 cut(s) 472, 552, 1845
BsmAI GTCTC 2 cut(s) 447, 1405
BsmFI GGGAC 1 cut(s) 1770
BsmI GAATGC 3 cut(s) 289, 336, 1852
BsnI GGCC 6 cut(s) 228, 509, 1101, 1126, 1607, 1640
Bsp120I GGGCCC 1 cut(s) 1605
Bsp1286I GDGCHC 4 cut(s) 157, 1314, 1541, 1609
BspACI CCGC 3 cut(s) 157, 314, 400
BspANI GGCC 6 cut(s) 228, 509, 1101, 1126, 1607, 1640
BspCNI CTCAG 4 cut(s) 310, 451, 1585, 1790
BspDI ATCGAT 1 cut(s) 808
BspFNI CGCG 1 cut(s) 1425
BspHI TCATGA 1 cut(s) 1270
BspLI GGNNCC 2 cut(s) 1203, 1607
BspMAI CTGCAG 3 cut(s) 847, 1078, 1690
BspMI ACCTGC 1 cut(s) 1426
BspPI GGATC 7 cut(s) 28, 578, 775, 1139, 1196, 1209, 1352
BspTI CTTAAG 1 cut(s) 1630
BsrDI GCAATG 1 cut(s) 1678
BsrI ACTGG 4 cut(s) 481, 539, 963, 1641
BssECI CCNNGG 6 cut(s) 504, 784, 924, 1127, 1315, 1316
BssSI CACGAG 1 cut(s) 335
BssT1I CCWWGG 2 cut(s) 504, 924
Bst2BI CACGAG 1 cut(s) 335
Bst2UI CCWGG 5 cut(s) 222, 762, 785, 1128, 1317
Bst4CI ACNGT 4 cut(s) 274, 611, 817, 1523
Bst6I CTCTTC 3 cut(s) 144, 1044, 1809
BstAFI CTTAAG 1 cut(s) 1630
BstAPI GCANNNNNTGC 1 cut(s) 1399
BstC8I GCNNGC 6 cut(s) 157, 843, 1352, 1387, 1391, 1395
BstDEI CTNAG 8 cut(s) 318, 459, 1149, 1593, 1601, 1777, 1824, 1863
BstF5I GGATG 4 cut(s) 826, 934, 1582, 1784
BstFNI CGCG 1 cut(s) 1425
BstHHI GCGC 1 cut(s) 1425
BstMAI GTCTC 2 cut(s) 447, 1405
BstMWI GCNNNNNNNGC 6 cut(s) 399, 506, 737, 1399, 1613, 1622
BstNI CCWGG 5 cut(s) 222, 762, 785, 1128, 1317
BstNSI RCATGY 2 cut(s) 1300, 1393
BstSCI CCNGG 5 cut(s) 220, 760, 783, 1126, 1315
BstSFI CTRYAG 3 cut(s) 843, 1074, 1686
BstSLI GKGCMC 2 cut(s) 157, 1609
BstUI CGCG 1 cut(s) 1425
BstX2I RGATCY 3 cut(s) 1131, 1201, 1344
BstXI CCANNNNNNTGG 1 cut(s) 627
BstYI RGATCY 3 cut(s) 1131, 1201, 1344
Bsu15I ATCGAT 1 cut(s) 808
BsuRI GGCC 6 cut(s) 228, 509, 1101, 1126, 1607, 1640
BsuTUI ATCGAT 1 cut(s) 808
BtsCI GGATG 4 cut(s) 826, 934, 1582, 1784
BtsIMutI CAGTG 4 cut(s) 488, 822, 1528, 1648
BveI ACCTGC 1 cut(s) 1426
Cac8I GCNNGC 6 cut(s) 157, 843, 1352, 1387, 1391, 1395
CaiI CAGNNNCTG 1 cut(s) 958
CciI TCATGA 1 cut(s) 1270
CfoI GCGC 1 cut(s) 1425
Cfr13I GGNCC 6 cut(s) 227, 463, 537, 1099, 1605, 1606
ClaI ATCGAT 1 cut(s) 808
CseI GACGC 1 cut(s) 1575
Csp6I GTAC 2 cut(s) 704, 1439
CviAII CATG 7 cut(s) 268, 1103, 1271, 1297, 1390, 1507, 1532
CviQI GTAC 2 cut(s) 704, 1439
DdeI CTNAG 8 cut(s) 318, 459, 1149, 1593, 1601, 1777, 1824, 1863
DraI TTTAAA 1 cut(s) 1518
DraIII CACNNNGTG 1 cut(s) 1399
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 1638
Eam1104I CTCTTC 3 cut(s) 144, 1044, 1809
EarI CTCTTC 3 cut(s) 144, 1044, 1809
Ecl136II GAGCTC 1 cut(s) 1539
Eco130I CCWWGG 2 cut(s) 504, 924
Eco147I AGGCCT 1 cut(s) 509
Eco24I GRGCYC 2 cut(s) 1541, 1609
Eco32I GATATC 2 cut(s) 91, 456
Eco47I GGWCC 2 cut(s) 463, 537
Eco53kI GAGCTC 1 cut(s) 1539
Eco57I CTGAAG 4 cut(s) 413, 488, 954, 1833
EcoICRI GAGCTC 1 cut(s) 1539
EcoO109I RGGNCCY 3 cut(s) 463, 1605, 1606
EcoRII CCWGG 5 cut(s) 220, 760, 783, 1126, 1315
EcoRV GATATC 2 cut(s) 91, 456
EcoT14I CCWWGG 2 cut(s) 504, 924
EcoT38I GRGCYC 2 cut(s) 1541, 1609
ErhI CCWWGG 2 cut(s) 504, 924
FaeI CATG 7 cut(s) 271, 1106, 1274, 1300, 1393, 1510, 1535
FalI AAGNNNNNCTT 4 cut(s) 690, 722, 1712, 1744
FaqI GGGAC 1 cut(s) 1770
FatI CATG 7 cut(s) 267, 1102, 1270, 1296, 1389, 1506, 1531
FauI CCCGC 1 cut(s) 164
FokI GGATG 4 cut(s) 833, 941, 1589, 1771
FriOI GRGCYC 2 cut(s) 1541, 1609
FspBI CTAG 1 cut(s) 390
GlaI GCGC 1 cut(s) 1424
GsuI CTGGAG 2 cut(s) 933, 980
HaeIII GGCC 6 cut(s) 228, 509, 1101, 1126, 1607, 1640
HapII CCGG 2 cut(s) 29, 553
HgaI GACGC 1 cut(s) 1575
HhaI GCGC 1 cut(s) 1425
Hin1II CATG 7 cut(s) 271, 1106, 1274, 1300, 1393, 1510, 1535
Hin6I GCGC 1 cut(s) 1423
HinP1I GCGC 1 cut(s) 1423
HincII GTYRAC 2 cut(s) 532, 1678
HindII GTYRAC 2 cut(s) 532, 1678
HindIII AAGCTT 1 cut(s) 738
HpaII CCGG 2 cut(s) 29, 553
HphI GGTGA 3 cut(s) 268, 725, 1658
Hpy166II GTNNAC 4 cut(s) 532, 907, 1678, 1830
Hpy188III TCNNGA 6 cut(s) 912, 1112, 1271, 1490, 1811, 1839
Hpy8I GTNNAC 4 cut(s) 532, 907, 1678, 1830
HpyAV CCTTC 7 cut(s) 403, 747, 995, 1504, 1698, 1717, 1845
HpyCH4III ACNGT 4 cut(s) 274, 611, 817, 1523
HpyF10VI GCNNNNNNNGC 6 cut(s) 399, 506, 737, 1399, 1613, 1622
HpyF3I CTNAG 8 cut(s) 318, 459, 1149, 1593, 1601, 1777, 1824, 1863
Hsp92II CATG 7 cut(s) 271, 1106, 1274, 1300, 1393, 1510, 1535
HspAI GCGC 1 cut(s) 1423
LmnI GCTCC 3 cut(s) 1309, 1536, 1597
LweI GCATC 5 cut(s) 811, 814, 867, 1132, 1372
MaeI CTAG 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 274, 1707
MboII GAAGA 5 cut(s) 131, 419, 568, 1061, 1826
MfeI CAATTG 1 cut(s) 825
MflI RGATCY 3 cut(s) 1131, 1201, 1344
MhlI GDGCHC 4 cut(s) 157, 1314, 1541, 1609
MlsI TGGCCA 1 cut(s) 1640
MluCI AATT 7 cut(s) 167, 201, 589, 641, 825, 881, 1191
MluNI TGGCCA 1 cut(s) 1640
MlyI GAGTC 4 cut(s) 194, 347, 1407, 1801
MmeI TCCRAC 1 cut(s) 85
Mox20I TGGCCA 1 cut(s) 1640
MroXI GAANNNNTTC 1 cut(s) 1053
MscI TGGCCA 1 cut(s) 1640
MseI TTAA 8 cut(s) 99, 384, 669, 690, 887, 1035, 1517, 1631
MslI CAYNNNNRTG 1 cut(s) 1295
Msp20I TGGCCA 1 cut(s) 1640
MspA1I CMGCKG 2 cut(s) 74, 405
MspCI CTTAAG 1 cut(s) 1630
MspI CCGG 2 cut(s) 29, 553
MspR9I CCNGG 5 cut(s) 222, 762, 785, 1128, 1317
MunI CAATTG 1 cut(s) 825
Mva1269I GAATGC 3 cut(s) 289, 336, 1852
MvaI CCWGG 5 cut(s) 222, 762, 785, 1128, 1317
MvnI CGCG 1 cut(s) 1425
MwoI GCNNNNNNNGC 6 cut(s) 399, 506, 737, 1399, 1613, 1622
NlaIII CATG 7 cut(s) 271, 1106, 1274, 1300, 1393, 1510, 1535
NlaIV GGNNCC 2 cut(s) 1203, 1607
NmuCI GTSAC 1 cut(s) 274
NspI RCATGY 2 cut(s) 1300, 1393
PaeI GCATGC 1 cut(s) 1393
PagI TCATGA 1 cut(s) 1270
PasI CCCWGGG 1 cut(s) 1316
PceI AGGCCT 1 cut(s) 509
PciI ACATGT 1 cut(s) 1296
PctI GAATGC 3 cut(s) 289, 336, 1852
PdmI GAANNNNTTC 1 cut(s) 1053
PfeI GAWTC 7 cut(s) 32, 413, 491, 1081, 1108, 1715, 1781
PleI GAGTC 4 cut(s) 193, 346, 1407, 1800
PpsI GAGTC 4 cut(s) 193, 346, 1407, 1800
PpuMI RGGWCCY 1 cut(s) 463
PscI ACATGT 1 cut(s) 1296
PsiI TTATAA 1 cut(s) 683
Psp124BI GAGCTC 1 cut(s) 1541
Psp5II RGGWCCY 1 cut(s) 463
Psp6I CCWGG 5 cut(s) 220, 760, 783, 1126, 1315
PspGI CCWGG 5 cut(s) 220, 760, 783, 1126, 1315
PspN4I GGNNCC 2 cut(s) 1203, 1607
PspOMI GGGCCC 1 cut(s) 1605
PspPI GGNCC 6 cut(s) 227, 463, 537, 1099, 1605, 1606
PspPPI RGGWCCY 1 cut(s) 463
PstI CTGCAG 3 cut(s) 847, 1078, 1690
PstNI CAGNNNCTG 1 cut(s) 958
PsuI RGATCY 3 cut(s) 1131, 1201, 1344
PvuII CAGCTG 2 cut(s) 74, 405
RsaI GTAC 2 cut(s) 705, 1440
RsaNI GTAC 2 cut(s) 704, 1439
RseI CAYNNNNRTG 1 cut(s) 1295
SacI GAGCTC 1 cut(s) 1541
SaqAI TTAA 8 cut(s) 99, 384, 669, 690, 887, 1035, 1517, 1631
Sau96I GGNCC 6 cut(s) 227, 463, 537, 1099, 1605, 1606
ScaI AGTACT 1 cut(s) 705
SchI GAGTC 4 cut(s) 194, 347, 1407, 1801
ScrFI CCNGG 5 cut(s) 222, 762, 785, 1128, 1317
SduI GDGCHC 4 cut(s) 157, 1314, 1541, 1609
SfaNI GCATC 5 cut(s) 811, 814, 867, 1132, 1372
SfcI CTRYAG 3 cut(s) 843, 1074, 1686
SinI GGWCC 2 cut(s) 463, 537
SmiMI CAYNNNNRTG 1 cut(s) 1295
SmlI CTYRAG 4 cut(s) 653, 1304, 1540, 1630
SmoI CTYRAG 4 cut(s) 653, 1304, 1540, 1630
SphI GCATGC 1 cut(s) 1393
Sse9I AATT 7 cut(s) 167, 201, 589, 641, 825, 881, 1191
SseBI AGGCCT 1 cut(s) 509
SsiI CCGC 3 cut(s) 157, 314, 400
SspMI CTAG 1 cut(s) 390
SstI GAGCTC 1 cut(s) 1541
StuI AGGCCT 1 cut(s) 509
StyD4I CCNGG 5 cut(s) 220, 760, 783, 1126, 1315
StyI CCWWGG 2 cut(s) 504, 924
TaaI ACNGT 4 cut(s) 274, 611, 817, 1523
TaqI TCGA 7 cut(s) 188, 307, 494, 516, 808, 1230, 1713
TasI AATT 7 cut(s) 167, 201, 589, 641, 825, 881, 1191
TatI WGTACW 1 cut(s) 703
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 7 cut(s) 32, 413, 491, 1081, 1108, 1715, 1781
Tru1I TTAA 8 cut(s) 99, 384, 669, 690, 887, 1035, 1517, 1631
Tru9I TTAA 8 cut(s) 99, 384, 669, 690, 887, 1035, 1517, 1631
TscAI CASTG 4 cut(s) 488, 822, 1528, 1648
TseFI GTSAC 1 cut(s) 274
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 5 cut(s) 17, 26, 1461, 1523, 1773
TspRI CASTG 4 cut(s) 488, 822, 1528, 1648
Vha464I CTTAAG 1 cut(s) 1630
VpaK11BI GGWCC 2 cut(s) 463, 537
XapI RAATTY 2 cut(s) 201, 1191
XceI RCATGY 2 cut(s) 1300, 1393
XcmI CCANNNNNNNNNTGG 2 cut(s) 627, 931
XmnI GAANNNNTTC 1 cut(s) 1053
XspI CTAG 1 cut(s) 390
ZrmI AGTACT 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.