MD05G1100300.v1.1

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014819
Physical Location & Seq
Forward (+)
20841577 .. 20843243
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1100300.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGCGGAGTGGTATAACCTTACATGTTCAACCTCCGCGAAGATTGGAGGACGATCAAGTGCAGCTGTTGCAGGATCGTACACATACAAGAATGCCACCTTCAACTGCCGAATTGATACGGATTCAAAAGTTACTGGAACATTAGGTACGAAGTTTCCGTCATGCACAAAGACTTCAGCTTCATTCAGCTTGCCGGAGTACAAGTCTAGCAAGCTCCGGTTTCAAACACGCCAAGAATATGGTGCCGCAGCTGTGTCTGTTTCTCTGTGCCAGTCCCCTGCAATCAACCTTTCAGCAACCGTCGGTAGTGCAAGCTTTGTCATCGGTATGGAGGCAGAATACAAGACCGCTTCCAGCACTTTCTCCAAATGTAATGCAGGCATTAGCATGAAGAGTCTCAATTCTGATGCTTCAATAATTCTGGGAAACAAGGGTGGCTTGGTAACAGCATCGTACGTTCATTATCTTGATCAAGAAAAGAAGAATGCAGCAGTGGTGGAGTTCACTCAGAACCTCTCAACAAAGAGAAGCACCTTGACAGTTGGAGGATCGTGCATGGTTGATCATCAAACAGTGGTGAAAGCTAGACTCGATGGTAGCGGAGATGTTAAGACTCTCCTGCAGTACAGTATTAGACCCAAGTCGCGCTTGTCTATCTCCGGCGAGTTCAACACCAAGGCACTTGACAGGATTCCTAAAATTGGACTGGCACTTTCTCTTGCCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Porin_3

Protein Analysis

243

Amino Acids

25.68

Weight (kDa)

9.48

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 11 - 235 6.7e-37 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014819)

Species Orthologous Gene IDs
malus_domestica MD05G1100000.v1.1 MD05G1100300.v1.1
prunus_persica Prupe.8G144700_v2.0.a1
pyrus_communis pycom05g09890
rosa_chinensis RchiOBHm_Chr6g0253791
rosa_laevigata RLG00000014970
rosa_multiflora Rmu_sc0006323.1_g000019
rosa_roxburghii Rroxscaffold_7G00211520
rosa_rugosa Rorug05G0547200 Rorug05G0547200
rosa_samantha Rh6AG062800 Rh6BG056100 Rh6CG055600 Rh6CG056200 Rh6DG053200 Rh6DG054000
rosa_wichuraiana Rw6G005590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 242
AccII CGCG 2 cut(s) 38, 646
AciI CCGC 5 cut(s) 5, 36, 246, 348, 600
AclWI GGATC 2 cut(s) 82, 556
AcuI CTGAAG 1 cut(s) 159
AfaI GTAC 5 cut(s) 80, 148, 200, 455, 626
AfiI CCNNNNNNNGG 1 cut(s) 701
AflIII ACRYGT 1 cut(s) 23
AgsI TTSAA 6 cut(s) 30, 103, 126, 224, 414, 670
AluBI AGCT 7 cut(s) 65, 179, 189, 214, 251, 315, 584
AluI AGCT 7 cut(s) 65, 179, 189, 214, 251, 315, 584
Alw26I GTCTC 1 cut(s) 401
AlwI GGATC 2 cut(s) 82, 556
ApeKI GCWGC 3 cut(s) 62, 248, 488
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
AspLEI GCGC 1 cut(s) 648
AsuHPI GGTGA 1 cut(s) 589
BanI GGYRCC 1 cut(s) 242
BbvI GCAGC 3 cut(s) 74, 260, 500
BccI CCATC 1 cut(s) 587
BclI TGATCA 2 cut(s) 469, 562
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 2 cut(s) 207, 585
BfmI CTRYAG 1 cut(s) 620
BisI GCNGC 4 cut(s) 63, 246, 249, 489
BlsI GCNGC 4 cut(s) 64, 247, 250, 490
BmiI GGNNCC 1 cut(s) 244
BmsI GCATC 2 cut(s) 397, 458
BoxI GACNNNNGTC 1 cut(s) 640
BsaJI CCNNGG 1 cut(s) 675
BsaWI WCCGGW 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 701
Bse1I ACTGG 3 cut(s) 139, 271, 711
BseDI CCNNGG 1 cut(s) 675
BseLI CCNNNNNNNGG 1 cut(s) 701
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 3 cut(s) 139, 271, 711
BseXI GCAGC 3 cut(s) 74, 260, 500
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 2 cut(s) 38, 646
BshNI GGYRCC 1 cut(s) 242
BsiSI CCGG 3 cut(s) 194, 217, 660
BsiWI CGTACG 1 cut(s) 453
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 1 cut(s) 701
BsmAI GTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 2 cut(s) 97, 490
Bsp143I GATC 5 cut(s) 53, 74, 469, 548, 562
BspACI CCGC 5 cut(s) 5, 36, 246, 348, 600
BspCNI CTCAG 1 cut(s) 520
BspFNI CGCG 2 cut(s) 38, 646
BspLI GGNNCC 1 cut(s) 244
BspMAI CTGCAG 1 cut(s) 624
BspPI GGATC 2 cut(s) 82, 556
BspT107I GGYRCC 1 cut(s) 242
BsrI ACTGG 3 cut(s) 139, 271, 711
BssECI CCNNGG 1 cut(s) 675
BssMI GATC 5 cut(s) 53, 74, 469, 548, 562
BssT1I CCWWGG 1 cut(s) 675
Bst4CI ACNGT 4 cut(s) 301, 541, 574, 629
Bst6I CTCTTC 1 cut(s) 386
BstAPI GCANNNNNTGC 1 cut(s) 68
BstC8I GCNNGC 4 cut(s) 191, 212, 313, 379
BstDEI CTNAG 1 cut(s) 507
BstFNI CGCG 2 cut(s) 38, 646
BstHHI GCGC 1 cut(s) 648
BstKTI GATC 5 cut(s) 56, 77, 472, 551, 565
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 5 cut(s) 53, 74, 469, 548, 562
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNSI RCATGY 1 cut(s) 27
BstPAI GACNNNNGTC 1 cut(s) 640
BstSFI CTRYAG 1 cut(s) 620
BstUI CGCG 2 cut(s) 38, 646
BstV1I GCAGC 3 cut(s) 74, 260, 500
BstXI CCANNNNNNTGG 1 cut(s) 239
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 498, 579
Cac8I GCNNGC 4 cut(s) 191, 212, 313, 379
CfoI GCGC 1 cut(s) 648
Csp6I GTAC 5 cut(s) 79, 147, 199, 454, 625
CviAII CATG 4 cut(s) 24, 162, 388, 556
CviJI RGCY 8 cut(s) 65, 179, 189, 214, 251, 315, 438, 584
CviKI_1 RGCY 8 cut(s) 65, 179, 189, 214, 251, 315, 438, 584
CviQI GTAC 5 cut(s) 79, 147, 199, 454, 625
DdeI CTNAG 1 cut(s) 507
DpnI GATC 5 cut(s) 55, 76, 471, 550, 564
DpnII GATC 5 cut(s) 53, 74, 469, 548, 562
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 675
Eco57I CTGAAG 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 675
ErhI CCWWGG 1 cut(s) 675
FaeI CATG 4 cut(s) 27, 165, 391, 559
FaiI YATR 8 cut(s) 15, 25, 85, 163, 240, 329, 389, 557
FalI AAGNNNNNCTT 4 cut(s) 422, 454, 632, 664
FaqI GGGAC 1 cut(s) 259
FatI CATG 4 cut(s) 23, 161, 387, 555
FbaI TGATCA 2 cut(s) 469, 562
Fnu4HI GCNGC 4 cut(s) 63, 246, 249, 489
Fsp4HI GCNGC 4 cut(s) 63, 246, 249, 489
FspBI CTAG 2 cut(s) 207, 585
GlaI GCGC 1 cut(s) 647
GluI GCNGC 4 cut(s) 63, 246, 249, 489
HapII CCGG 3 cut(s) 194, 217, 660
HhaI GCGC 1 cut(s) 648
Hin1II CATG 4 cut(s) 27, 165, 391, 559
Hin6I GCGC 1 cut(s) 646
HinP1I GCGC 1 cut(s) 646
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 5 cut(s) 122, 394, 588, 613, 691
HpaII CCGG 3 cut(s) 194, 217, 660
HphI GGTGA 1 cut(s) 589
Hpy166II GTNNAC 2 cut(s) 81, 504
Hpy188I TCNGA 2 cut(s) 406, 510
Hpy188III TCNNGA 2 cut(s) 467, 473
Hpy8I GTNNAC 2 cut(s) 81, 504
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 4 cut(s) 301, 541, 574, 629
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 9 cut(s) 62, 71, 165, 281, 311, 377, 488, 555, 622
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 507
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 4 cut(s) 27, 165, 391, 559
HspAI GCGC 1 cut(s) 646
Ksp22I TGATCA 2 cut(s) 469, 562
Kzo9I GATC 5 cut(s) 53, 74, 469, 548, 562
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 3 cut(s) 74, 260, 500
LweI GCATC 2 cut(s) 397, 458
MaeI CTAG 2 cut(s) 207, 585
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 2 cut(s) 130, 442
MalI GATC 5 cut(s) 55, 76, 471, 550, 564
MboI GATC 5 cut(s) 53, 74, 469, 548, 562
MboII GAAGA 3 cut(s) 52, 403, 493
MluCI AATT 4 cut(s) 111, 400, 417, 699
MlyI GAGTC 3 cut(s) 403, 582, 607
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 5 cut(s) 41, 43, 325, 524, 539
MseI TTAA 2 cut(s) 609, 727
MslI CAYNNNNRTG 2 cut(s) 326, 386
MspA1I CMGCKG 2 cut(s) 65, 251
MspI CCGG 3 cut(s) 194, 217, 660
Mva1269I GAATGC 2 cut(s) 97, 490
MvnI CGCG 2 cut(s) 38, 646
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 5 cut(s) 53, 74, 469, 548, 562
NlaIII CATG 4 cut(s) 27, 165, 391, 559
NlaIV GGNNCC 1 cut(s) 244
NspI RCATGY 1 cut(s) 27
PciI ACATGT 1 cut(s) 23
PcsI WCGNNNNNNNCGW 1 cut(s) 155
PctI GAATGC 2 cut(s) 97, 490
PfeI GAWTC 2 cut(s) 122, 691
Pfl23II CGTACG 1 cut(s) 453
PkrI GCNGC 4 cut(s) 64, 247, 250, 490
PleI GAGTC 3 cut(s) 402, 582, 607
PpsI GAGTC 3 cut(s) 402, 582, 607
PscI ACATGT 1 cut(s) 23
PshAI GACNNNNGTC 1 cut(s) 640
PspLI CGTACG 1 cut(s) 453
PspN4I GGNNCC 1 cut(s) 244
PsrI GAACNNNNNNTAC 2 cut(s) 130, 162
PstI CTGCAG 1 cut(s) 624
PvuII CAGCTG 2 cut(s) 65, 251
RsaI GTAC 5 cut(s) 80, 148, 200, 455, 626
RsaNI GTAC 5 cut(s) 79, 147, 199, 454, 625
RseI CAYNNNNRTG 2 cut(s) 326, 386
SaqAI TTAA 2 cut(s) 609, 727
SatI GCNGC 4 cut(s) 63, 246, 249, 489
Sau3AI GATC 5 cut(s) 53, 74, 469, 548, 562
SchI GAGTC 3 cut(s) 403, 582, 607
SfaNI GCATC 2 cut(s) 397, 458
SfcI CTRYAG 1 cut(s) 620
SmiMI CAYNNNNRTG 2 cut(s) 326, 386
Sse9I AATT 4 cut(s) 111, 400, 417, 699
SsiI CCGC 5 cut(s) 5, 36, 246, 348, 600
SspMI CTAG 2 cut(s) 207, 585
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 4 cut(s) 301, 541, 574, 629
TaiI ACGT 1 cut(s) 459
TaqI TCGA 1 cut(s) 591
TasI AATT 4 cut(s) 111, 400, 417, 699
TatI WGTACW 2 cut(s) 198, 624
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 2 cut(s) 122, 691
Tru1I TTAA 2 cut(s) 609, 727
Tru9I TTAA 2 cut(s) 609, 727
TscAI CASTG 2 cut(s) 498, 579
TseI GCWGC 3 cut(s) 62, 248, 488
TspDTI ATGAA 3 cut(s) 171, 404, 449
TspGWI ACGGA 2 cut(s) 134, 147
TspRI CASTG 2 cut(s) 498, 579
XceI RCATGY 1 cut(s) 27
XspI CTAG 2 cut(s) 207, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.