Belongs to the 'GDSL' lipolytic enzyme family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1358 |
| Acc36I | ACCTGC | 1 cut(s) | 928 |
| AccB1I | GGYRCC | 1 cut(s) | 1426 |
| AciI | CCGC | 5 cut(s) | 173, 377, 547, 739, 996 |
| AclWI | GGATC | 3 cut(s) | 482, 1160, 1173 |
| AcsI | RAATTY | 2 cut(s) | 16, 1186 |
| AcuI | CTGAAG | 1 cut(s) | 116 |
| AfaI | GTAC | 4 cut(s) | 601, 729, 1230, 1365 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 48, 695, 1175, 1373 |
| AgsI | TTSAA | 6 cut(s) | 15, 364, 403, 801, 1199, 1334 |
| AjnI | CCWGG | 3 cut(s) | 246, 909, 1366 |
| AluBI | AGCT | 7 cut(s) | 217, 431, 713, 748, 1111, 1126, 1202 |
| AluI | AGCT | 7 cut(s) | 217, 431, 713, 748, 1111, 1126, 1202 |
| AlwI | GGATC | 3 cut(s) | 482, 1160, 1173 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 539 |
| Ama87I | CYCGRG | 1 cut(s) | 46 |
| AoxI | GGCC | 2 cut(s) | 39, 907 |
| ApeKI | GCWGC | 4 cut(s) | 85, 142, 214, 1108 |
| ApoI | RAATTY | 2 cut(s) | 16, 1186 |
| AseI | ATTAAT | 1 cut(s) | 884 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 471, 1353 |
| AsuHPI | GGTGA | 3 cut(s) | 1031, 1106, 1388 |
| AvaI | CYCGRG | 1 cut(s) | 46 |
| BamHI | GGATCC | 1 cut(s) | 1165 |
| BanI | GGYRCC | 1 cut(s) | 1426 |
| BauI | CACGAG | 2 cut(s) | 492, 690 |
| BbsI | GAAGAC | 1 cut(s) | 1356 |
| BbvCI | CCTCAGC | 1 cut(s) | 118 |
| BbvI | GCAGC | 4 cut(s) | 97, 154, 226, 1120 |
| BccI | CCATC | 3 cut(s) | 71, 321, 1441 |
| BciT130I | CCWGG | 3 cut(s) | 248, 911, 1368 |
| BclI | TGATCA | 3 cut(s) | 223, 610, 1414 |
| BfaI | CTAG | 2 cut(s) | 432, 555 |
| BfuAI | ACCTGC | 1 cut(s) | 928 |
| BisI | GCNGC | 5 cut(s) | 86, 143, 174, 215, 1109 |
| BlsI | GCNGC | 5 cut(s) | 87, 144, 175, 216, 1110 |
| Bme1390I | CCNGG | 3 cut(s) | 248, 911, 1368 |
| BmeT110I | CYCGRG | 1 cut(s) | 46 |
| BmiI | GGNNCC | 2 cut(s) | 1167, 1428 |
| BmrFI | CCNGG | 3 cut(s) | 248, 911, 1368 |
| BmrI | ACTGGG | 1 cut(s) | 963 |
| BmsI | GCATC | 2 cut(s) | 310, 526 |
| BmuI | ACTGGG | 1 cut(s) | 963 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1101 |
| BpiI | GAAGAC | 1 cut(s) | 1356 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 29, 61 |
| Bpu10I | CCTNAGC | 1 cut(s) | 118 |
| BpuEI | CTTGAG | 1 cut(s) | 1257 |
| BsaAI | YACGTR | 1 cut(s) | 836 |
| BsaJI | CCNNGG | 2 cut(s) | 45, 910 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 48, 695, 1175, 1373 |
| Bse1I | ACTGG | 2 cut(s) | 958, 1342 |
| BseBI | CCWGG | 3 cut(s) | 248, 911, 1368 |
| BseDI | CCNNGG | 2 cut(s) | 45, 910 |
| BseGI | GGATG | 3 cut(s) | 82, 313, 457 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 48, 695, 1175, 1373 |
| BseMII | CTCAG | 2 cut(s) | 132, 1317 |
| BseNI | ACTGG | 2 cut(s) | 958, 1342 |
| BseRI | GAGGAG | 1 cut(s) | 34 |
| BseXI | GCAGC | 4 cut(s) | 97, 154, 226, 1120 |
| BsgI | GTGCAG | 1 cut(s) | 1251 |
| BshFI | GGCC | 2 cut(s) | 41, 909 |
| BshNI | GGYRCC | 1 cut(s) | 1426 |
| BsiHKCI | CYCGRG | 1 cut(s) | 46 |
| BslFI | GGGAC | 1 cut(s) | 927 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 48, 695, 1175, 1373 |
| BsmFI | GGGAC | 1 cut(s) | 927 |
| BsmI | GAATGC | 2 cut(s) | 471, 1039 |
| BsnI | GGCC | 2 cut(s) | 41, 909 |
| BsoBI | CYCGRG | 1 cut(s) | 46 |
| Bsp1407I | TGTACA | 1 cut(s) | 727 |
| Bsp143I | GATC | 5 cut(s) | 223, 487, 610, 1165, 1414 |
| BspACI | CCGC | 5 cut(s) | 173, 377, 547, 739, 996 |
| BspANI | GGCC | 2 cut(s) | 41, 909 |
| BspCNI | CTCAG | 2 cut(s) | 131, 1318 |
| BspHI | TCATGA | 2 cut(s) | 220, 295 |
| BspLI | GGNNCC | 2 cut(s) | 1167, 1428 |
| BspMI | ACCTGC | 1 cut(s) | 928 |
| BspPI | GGATC | 3 cut(s) | 482, 1160, 1173 |
| BspT107I | GGYRCC | 1 cut(s) | 1426 |
| BsrGI | TGTACA | 1 cut(s) | 727 |
| BsrI | ACTGG | 2 cut(s) | 958, 1342 |
| BssECI | CCNNGG | 2 cut(s) | 45, 910 |
| BssMI | GATC | 5 cut(s) | 223, 487, 610, 1165, 1414 |
| BssSI | CACGAG | 2 cut(s) | 492, 690 |
| Bst2BI | CACGAG | 2 cut(s) | 492, 690 |
| Bst2UI | CCWGG | 3 cut(s) | 248, 911, 1368 |
| Bst4CI | ACNGT | 3 cut(s) | 193, 577, 604 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1156 |
| BstAUI | TGTACA | 1 cut(s) | 727 |
| BstBAI | YACGTR | 1 cut(s) | 836 |
| BstC8I | GCNNGC | 3 cut(s) | 375, 636, 1044 |
| BstDEI | CTNAG | 3 cut(s) | 118, 744, 1326 |
| BstF5I | GGATG | 3 cut(s) | 82, 313, 457 |
| BstKTI | GATC | 5 cut(s) | 226, 490, 613, 1168, 1417 |
| BstMBI | GATC | 5 cut(s) | 223, 487, 610, 1165, 1414 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 142, 170, 754, 1043, 1156, 1199 |
| BstNI | CCWGG | 3 cut(s) | 248, 911, 1368 |
| BstNSI | RCATGY | 4 cut(s) | 136, 1050, 1154, 1286 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1101 |
| BstSCI | CCNGG | 3 cut(s) | 246, 909, 1366 |
| BstV1I | GCAGC | 4 cut(s) | 97, 154, 226, 1120 |
| BstV2I | GAAGAC | 1 cut(s) | 1356 |
| BstX2I | RGATCY | 1 cut(s) | 1165 |
| BstYI | RGATCY | 1 cut(s) | 1165 |
| BsuRI | GGCC | 2 cut(s) | 41, 909 |
| BtsCI | GGATG | 3 cut(s) | 82, 313, 457 |
| BveI | ACCTGC | 1 cut(s) | 928 |
| Cac8I | GCNNGC | 3 cut(s) | 375, 636, 1044 |
| CaiI | CAGNNNCTG | 1 cut(s) | 539 |
| CciI | TCATGA | 2 cut(s) | 220, 295 |
| Csp6I | GTAC | 4 cut(s) | 600, 728, 1229, 1364 |
| CviQI | GTAC | 4 cut(s) | 600, 728, 1229, 1364 |
| DdeI | CTNAG | 3 cut(s) | 118, 744, 1326 |
| DpnI | GATC | 5 cut(s) | 225, 489, 612, 1167, 1416 |
| DpnII | GATC | 5 cut(s) | 223, 487, 610, 1165, 1414 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1358 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1358 |
| Eco147I | AGGCCT | 1 cut(s) | 41 |
| Eco32I | GATATC | 1 cut(s) | 679 |
| Eco57I | CTGAAG | 1 cut(s) | 116 |
| Eco88I | CYCGRG | 1 cut(s) | 46 |
| EcoRI | GAATTC | 1 cut(s) | 1186 |
| EcoRII | CCWGG | 3 cut(s) | 246, 909, 1366 |
| EcoRV | GATATC | 1 cut(s) | 679 |
| EcoT22I | ATGCAT | 2 cut(s) | 138, 825 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 459, 491 |
| FaqI | GGGAC | 1 cut(s) | 927 |
| FbaI | TGATCA | 3 cut(s) | 223, 610, 1414 |
| Fnu4HI | GCNGC | 5 cut(s) | 86, 143, 174, 215, 1109 |
| FokI | GGATG | 3 cut(s) | 89, 300, 464 |
| Fsp4HI | GCNGC | 5 cut(s) | 86, 143, 174, 215, 1109 |
| FspBI | CTAG | 2 cut(s) | 432, 555 |
| GluI | GCNGC | 5 cut(s) | 86, 143, 174, 215, 1109 |
| HaeIII | GGCC | 2 cut(s) | 41, 909 |
| HincII | GTYRAC | 1 cut(s) | 244 |
| HindII | GTYRAC | 1 cut(s) | 244 |
| HindIII | AAGCTT | 1 cut(s) | 746 |
| HinfI | GANTC | 4 cut(s) | 251, 839, 1075, 1235 |
| HpaI | GTTAAC | 1 cut(s) | 244 |
| HphI | GGTGA | 3 cut(s) | 1031, 1106, 1388 |
| Hpy166II | GTNNAC | 2 cut(s) | 244, 442 |
| Hpy188I | TCNGA | 8 cut(s) | 270, 451, 500, 810, 877, 1144, 1327, 1344 |
| Hpy188III | TCNNGA | 7 cut(s) | 63, 221, 296, 317, 608, 1063, 1418 |
| Hpy8I | GTNNAC | 2 cut(s) | 244, 442 |
| HpyAV | CCTTC | 8 cut(s) | 429, 456, 461, 577, 582, 1085, 1216, 1415 |
| HpyCH4III | ACNGT | 3 cut(s) | 193, 577, 604 |
| HpyCH4IV | ACGT | 1 cut(s) | 835 |
| HpyCH4V | TGCA | 6 cut(s) | 85, 136, 823, 1268, 1282, 1293 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 142, 170, 754, 1043, 1156, 1199 |
| HpyF3I | CTNAG | 3 cut(s) | 118, 744, 1326 |
| HpySE526I | ACGT | 1 cut(s) | 835 |
| Ksp22I | TGATCA | 3 cut(s) | 223, 610, 1414 |
| KspAI | GTTAAC | 1 cut(s) | 244 |
| Kzo9I | GATC | 5 cut(s) | 223, 487, 610, 1165, 1414 |
| Lsp1109I | GCAGC | 4 cut(s) | 97, 154, 226, 1120 |
| LweI | GCATC | 2 cut(s) | 310, 526 |
| MaeI | CTAG | 2 cut(s) | 432, 555 |
| MaeII | ACGT | 1 cut(s) | 835 |
| MaeIII | GTNAC | 4 cut(s) | 197, 407, 1094, 1318 |
| MalI | GATC | 5 cut(s) | 225, 489, 612, 1167, 1416 |
| MboI | GATC | 5 cut(s) | 223, 487, 610, 1165, 1414 |
| MboII | GAAGA | 4 cut(s) | 109, 529, 647, 1361 |
| MflI | RGATCY | 1 cut(s) | 1165 |
| MluCI | AATT | 6 cut(s) | 16, 459, 623, 885, 1113, 1186 |
| MlyI | GAGTC | 2 cut(s) | 245, 1244 |
| MmeI | TCCRAC | 3 cut(s) | 974, 1143, 1263 |
| Mph1103I | ATGCAT | 2 cut(s) | 138, 825 |
| MroXI | GAANNNNTTC | 2 cut(s) | 471, 1353 |
| MseI | TTAA | 3 cut(s) | 243, 285, 884 |
| MslI | CAYNNNNRTG | 2 cut(s) | 75, 318 |
| MspR9I | CCNGG | 3 cut(s) | 248, 911, 1368 |
| Mva1269I | GAATGC | 2 cut(s) | 471, 1039 |
| MvaI | CCWGG | 3 cut(s) | 248, 911, 1368 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 142, 170, 754, 1043, 1156, 1199 |
| NdeII | GATC | 5 cut(s) | 223, 487, 610, 1165, 1414 |
| NlaIV | GGNNCC | 2 cut(s) | 1167, 1428 |
| NmuCI | GTSAC | 3 cut(s) | 197, 1094, 1318 |
| NsiI | ATGCAT | 2 cut(s) | 138, 825 |
| NspI | RCATGY | 4 cut(s) | 136, 1050, 1154, 1286 |
| PagI | TCATGA | 2 cut(s) | 220, 295 |
| PceI | AGGCCT | 1 cut(s) | 41 |
| PctI | GAATGC | 2 cut(s) | 471, 1039 |
| PdmI | GAANNNNTTC | 2 cut(s) | 471, 1353 |
| PfeI | GAWTC | 2 cut(s) | 839, 1075 |
| PkrI | GCNGC | 5 cut(s) | 87, 144, 175, 216, 1110 |
| PleI | GAGTC | 2 cut(s) | 245, 1243 |
| PpsI | GAGTC | 2 cut(s) | 245, 1243 |
| Ppu21I | YACGTR | 1 cut(s) | 836 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1101 |
| PshBI | ATTAAT | 1 cut(s) | 884 |
| Psp6I | CCWGG | 3 cut(s) | 246, 909, 1366 |
| PspGI | CCWGG | 3 cut(s) | 246, 909, 1366 |
| PspN4I | GGNNCC | 2 cut(s) | 1167, 1428 |
| PstNI | CAGNNNCTG | 1 cut(s) | 539 |
| PsuI | RGATCY | 1 cut(s) | 1165 |
| RsaI | GTAC | 4 cut(s) | 601, 729, 1230, 1365 |
| RsaNI | GTAC | 4 cut(s) | 600, 728, 1229, 1364 |
| RseI | CAYNNNNRTG | 2 cut(s) | 75, 318 |
| SaqAI | TTAA | 3 cut(s) | 243, 285, 884 |
| SatI | GCNGC | 5 cut(s) | 86, 143, 174, 215, 1109 |
| Sau3AI | GATC | 5 cut(s) | 223, 487, 610, 1165, 1414 |
| SchI | GAGTC | 2 cut(s) | 245, 1244 |
| ScrFI | CCNGG | 3 cut(s) | 248, 911, 1368 |
| SfaNI | GCATC | 2 cut(s) | 310, 526 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 75, 318 |
| SmlI | CTYRAG | 1 cut(s) | 1272 |
| SmoI | CTYRAG | 1 cut(s) | 1272 |
| Sse9I | AATT | 6 cut(s) | 16, 459, 623, 885, 1113, 1186 |
| SseBI | AGGCCT | 1 cut(s) | 41 |
| SsiI | CCGC | 5 cut(s) | 173, 377, 547, 739, 996 |
| SspMI | CTAG | 2 cut(s) | 432, 555 |
| StuI | AGGCCT | 1 cut(s) | 41 |
| StyD4I | CCNGG | 3 cut(s) | 246, 909, 1366 |
| TaaI | ACNGT | 3 cut(s) | 193, 577, 604 |
| TaiI | ACGT | 1 cut(s) | 838 |
| TaqII | GACCGA | 1 cut(s) | 894 |
| TasI | AATT | 6 cut(s) | 16, 459, 623, 885, 1113, 1186 |
| TatI | WGTACW | 1 cut(s) | 727 |
| TauI | GCSGC | 1 cut(s) | 176 |
| TfiI | GAWTC | 2 cut(s) | 839, 1075 |
| Tru1I | TTAA | 3 cut(s) | 243, 285, 884 |
| Tru9I | TTAA | 3 cut(s) | 243, 285, 884 |
| TseFI | GTSAC | 3 cut(s) | 197, 1094, 1318 |
| TseI | GCWGC | 4 cut(s) | 85, 142, 214, 1108 |
| Tsp45I | GTSAC | 3 cut(s) | 197, 1094, 1318 |
| VspI | ATTAAT | 1 cut(s) | 884 |
| XapI | RAATTY | 2 cut(s) | 16, 1186 |
| XceI | RCATGY | 4 cut(s) | 136, 1050, 1154, 1286 |
| XmnI | GAANNNNTTC | 2 cut(s) | 471, 1353 |
| XspI | CTAG | 2 cut(s) | 432, 555 |
| Zsp2I | ATGCAT | 2 cut(s) | 138, 825 |