Belongs to the 'GDSL' lipolytic enzyme family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 485, 580 |
| AccBSI | CCGCTC | 1 cut(s) | 418 |
| AccII | CGCG | 1 cut(s) | 1371 |
| AciI | CCGC | 3 cut(s) | 418, 518, 587 |
| AcoI | YGGCCR | 1 cut(s) | 1551 |
| AcsI | RAATTY | 6 cut(s) | 596, 640, 665, 895, 959, 1399 |
| AcuI | CTGAAG | 3 cut(s) | 186, 914, 968 |
| AcyI | GRCGYC | 1 cut(s) | 434 |
| AdeI | CACNNNGTG | 1 cut(s) | 374 |
| AfaI | GTAC | 1 cut(s) | 500 |
| AflIII | ACRYGT | 2 cut(s) | 1349, 1570 |
| AgeI | ACCGGT | 1 cut(s) | 503 |
| AhlI | ACTAGT | 1 cut(s) | 58 |
| AjnI | CCWGG | 1 cut(s) | 1548 |
| AleI | CACNNNNGTG | 2 cut(s) | 1103, 1339 |
| Alw21I | GWGCWC | 2 cut(s) | 249, 1100 |
| Alw26I | GTCTC | 2 cut(s) | 1215, 1349 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 89 |
| AoxI | GGCC | 2 cut(s) | 650, 1551 |
| ApeKI | GCWGC | 5 cut(s) | 359, 439, 677, 1109, 1493 |
| ApoI | RAATTY | 6 cut(s) | 596, 640, 665, 895, 959, 1399 |
| AsiGI | ACCGGT | 1 cut(s) | 503 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 597 |
| AspLEI | GCGC | 1 cut(s) | 1371 |
| AspS9I | GGNCC | 2 cut(s) | 650, 1546 |
| AsuHPI | GGTGA | 3 cut(s) | 37, 283, 566 |
| AvaII | GGWCC | 1 cut(s) | 1546 |
| BalI | TGGCCA | 1 cut(s) | 1553 |
| BanI | GGYRCC | 2 cut(s) | 485, 580 |
| BanII | GRGCYC | 1 cut(s) | 249 |
| BbsI | GAAGAC | 2 cut(s) | 192, 554 |
| Bbv12I | GWGCWC | 2 cut(s) | 249, 1100 |
| BbvCI | CCTCAGC | 1 cut(s) | 354 |
| BbvI | GCAGC | 5 cut(s) | 346, 426, 689, 1096, 1505 |
| BccI | CCATC | 2 cut(s) | 391, 823 |
| BceAI | ACGGC | 1 cut(s) | 447 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1354, 1388 |
| BciT130I | CCWGG | 1 cut(s) | 1550 |
| BciVI | GTATCC | 1 cut(s) | 432 |
| BcoDI | GTCTC | 2 cut(s) | 1215, 1349 |
| BcuI | ACTAGT | 1 cut(s) | 58 |
| BfaI | CTAG | 4 cut(s) | 59, 137, 1322, 1362 |
| BfmI | CTRYAG | 1 cut(s) | 1607 |
| BfuI | GTATCC | 1 cut(s) | 432 |
| BisI | GCNGC | 6 cut(s) | 360, 440, 588, 678, 1110, 1494 |
| BlpI | GCTNAGC | 3 cut(s) | 333, 402, 1386 |
| BlsI | GCNGC | 6 cut(s) | 361, 441, 589, 679, 1111, 1495 |
| Bme1390I | CCNGG | 1 cut(s) | 1550 |
| Bme18I | GGWCC | 1 cut(s) | 1546 |
| BmgT120I | GGNCC | 2 cut(s) | 650, 1546 |
| BmiI | GGNNCC | 5 cut(s) | 458, 487, 582, 651, 978 |
| BmrFI | CCNGG | 1 cut(s) | 1550 |
| BmsI | GCATC | 4 cut(s) | 400, 619, 1353, 1420 |
| BpiI | GAAGAC | 2 cut(s) | 192, 554 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1169, 1201 |
| BpmI | CTGGAG | 2 cut(s) | 898, 995 |
| Bpu10I | CCTNAGC | 2 cut(s) | 354, 1186 |
| Bpu1102I | GCTNAGC | 3 cut(s) | 333, 402, 1386 |
| BpuEI | CTTGAG | 4 cut(s) | 263, 464, 477, 587 |
| Bsa29I | ATCGAT | 1 cut(s) | 723 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1470 |
| BsaHI | GRCGYC | 1 cut(s) | 434 |
| BsaI | GGTCTC | 1 cut(s) | 1349 |
| BsaJI | CCNNGG | 3 cut(s) | 314, 772, 1337 |
| BsaWI | WCCGGW | 1 cut(s) | 503 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1195, 1225 |
| Bse118I | RCCGGY | 1 cut(s) | 503 |
| Bse1I | ACTGG | 2 cut(s) | 556, 700 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1470 |
| BseBI | CCWGG | 1 cut(s) | 1550 |
| BseCI | ATCGAT | 1 cut(s) | 723 |
| BseDI | CCNNGG | 3 cut(s) | 314, 772, 1337 |
| BseGI | GGATG | 2 cut(s) | 30, 1447 |
| BseJI | GATNNNNATC | 1 cut(s) | 1470 |
| BseMII | CTCAG | 5 cut(s) | 324, 345, 385, 780, 1200 |
| BseNI | ACTGG | 2 cut(s) | 556, 700 |
| BseRI | GAGGAG | 2 cut(s) | 143, 1190 |
| BseXI | GCAGC | 5 cut(s) | 346, 426, 689, 1096, 1505 |
| BseYI | CCCAGC | 1 cut(s) | 70 |
| Bsh1236I | CGCG | 1 cut(s) | 1371 |
| BshFI | GGCC | 2 cut(s) | 652, 1553 |
| BshNI | GGYRCC | 2 cut(s) | 485, 580 |
| BshTI | ACCGGT | 1 cut(s) | 503 |
| BshVI | ATCGAT | 1 cut(s) | 723 |
| BsiHKAI | GWGCWC | 2 cut(s) | 249, 1100 |
| BsiSI | CCGG | 1 cut(s) | 504 |
| BslFI | GGGAC | 2 cut(s) | 196, 832 |
| BsmAI | GTCTC | 2 cut(s) | 1215, 1349 |
| BsmFI | GGGAC | 2 cut(s) | 196, 832 |
| BsnI | GGCC | 2 cut(s) | 652, 1553 |
| Bso31I | GGTCTC | 1 cut(s) | 1349 |
| Bsp1286I | GDGCHC | 2 cut(s) | 249, 1100 |
| Bsp1407I | TGTACA | 1 cut(s) | 498 |
| Bsp143I | GATC | 2 cut(s) | 447, 1515 |
| Bsp1720I | GCTNAGC | 3 cut(s) | 333, 402, 1386 |
| Bsp19I | CCATGG | 1 cut(s) | 1337 |
| BspACI | CCGC | 3 cut(s) | 418, 518, 587 |
| BspANI | GGCC | 2 cut(s) | 652, 1553 |
| BspCNI | CTCAG | 5 cut(s) | 325, 346, 384, 781, 1199 |
| BspDI | ATCGAT | 1 cut(s) | 723 |
| BspFNI | CGCG | 1 cut(s) | 1371 |
| BspLI | GGNNCC | 5 cut(s) | 458, 487, 582, 651, 978 |
| BspQI | GCTCTTC | 1 cut(s) | 165 |
| BspT107I | GGYRCC | 2 cut(s) | 485, 580 |
| BspTNI | GGTCTC | 1 cut(s) | 1349 |
| BsrBI | CCGCTC | 1 cut(s) | 418 |
| BsrFI | RCCGGY | 1 cut(s) | 503 |
| BsrGI | TGTACA | 1 cut(s) | 498 |
| BsrI | ACTGG | 2 cut(s) | 556, 700 |
| BssAI | RCCGGY | 1 cut(s) | 503 |
| BssECI | CCNNGG | 3 cut(s) | 314, 772, 1337 |
| BssMI | GATC | 2 cut(s) | 447, 1515 |
| BssNI | GRCGYC | 1 cut(s) | 434 |
| BssT1I | CCWWGG | 3 cut(s) | 314, 772, 1337 |
| Bst2UI | CCWGG | 1 cut(s) | 1550 |
| Bst4CI | ACNGT | 3 cut(s) | 907, 1102, 1258 |
| Bst6I | CTCTTC | 2 cut(s) | 165, 987 |
| BstACI | GRCGYC | 1 cut(s) | 434 |
| BstAUI | TGTACA | 1 cut(s) | 498 |
| BstC8I | GCNNGC | 2 cut(s) | 585, 1367 |
| BstDEI | CTNAG | 7 cut(s) | 333, 354, 371, 402, 789, 1186, 1386 |
| BstDSI | CCRYGG | 1 cut(s) | 1337 |
| BstF5I | GGATG | 2 cut(s) | 30, 1447 |
| BstFNI | CGCG | 1 cut(s) | 1371 |
| BstHHI | GCGC | 1 cut(s) | 1371 |
| BstKTI | GATC | 2 cut(s) | 450, 1518 |
| BstMAI | GTCTC | 2 cut(s) | 1215, 1349 |
| BstMBI | GATC | 2 cut(s) | 447, 1515 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 329, 359, 683, 692, 976 |
| BstNI | CCWGG | 1 cut(s) | 1550 |
| BstNSI | RCATGY | 2 cut(s) | 1353, 1574 |
| BstSCI | CCNGG | 1 cut(s) | 1548 |
| BstSFI | CTRYAG | 1 cut(s) | 1607 |
| BstUI | CGCG | 1 cut(s) | 1371 |
| BstV1I | GCAGC | 5 cut(s) | 346, 426, 689, 1096, 1505 |
| BstV2I | GAAGAC | 2 cut(s) | 192, 554 |
| Bsu15I | ATCGAT | 1 cut(s) | 723 |
| BsuI | GTATCC | 1 cut(s) | 432 |
| BsuRI | GGCC | 2 cut(s) | 652, 1553 |
| BsuTUI | ATCGAT | 1 cut(s) | 723 |
| BtgI | CCRYGG | 1 cut(s) | 1337 |
| BtsCI | GGATG | 2 cut(s) | 30, 1447 |
| BtsI | GCAGTG | 1 cut(s) | 804 |
| BtsIMutI | CAGTG | 3 cut(s) | 379, 804, 1098 |
| Cac8I | GCNNGC | 2 cut(s) | 585, 1367 |
| CaiI | CAGNNNCTG | 1 cut(s) | 89 |
| CfoI | GCGC | 1 cut(s) | 1371 |
| Cfr10I | RCCGGY | 1 cut(s) | 503 |
| Cfr13I | GGNCC | 2 cut(s) | 650, 1546 |
| ClaI | ATCGAT | 1 cut(s) | 723 |
| CseI | GACGC | 2 cut(s) | 423, 1360 |
| Csp6I | GTAC | 1 cut(s) | 499 |
| CspAI | ACCGGT | 1 cut(s) | 503 |
| CviAII | CATG | 6 cut(s) | 254, 1199, 1262, 1338, 1350, 1571 |
| CviQI | GTAC | 1 cut(s) | 499 |
| DdeI | CTNAG | 7 cut(s) | 333, 354, 371, 402, 789, 1186, 1386 |
| DpnI | GATC | 2 cut(s) | 449, 1517 |
| DpnII | GATC | 2 cut(s) | 447, 1515 |
| DraIII | CACNNNGTG | 1 cut(s) | 374 |
| EaeI | YGGCCR | 1 cut(s) | 1551 |
| Eam1104I | CTCTTC | 2 cut(s) | 165, 987 |
| EarI | CTCTTC | 2 cut(s) | 165, 987 |
| Ecl136II | GAGCTC | 1 cut(s) | 247 |
| Eco130I | CCWWGG | 3 cut(s) | 314, 772, 1337 |
| Eco24I | GRGCYC | 1 cut(s) | 249 |
| Eco31I | GGTCTC | 1 cut(s) | 1349 |
| Eco47I | GGWCC | 1 cut(s) | 1546 |
| Eco53kI | GAGCTC | 1 cut(s) | 247 |
| Eco57I | CTGAAG | 3 cut(s) | 186, 914, 968 |
| EcoICRI | GAGCTC | 1 cut(s) | 247 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1546 |
| EcoRI | GAATTC | 1 cut(s) | 959 |
| EcoRII | CCWGG | 1 cut(s) | 1548 |
| EcoT14I | CCWWGG | 3 cut(s) | 314, 772, 1337 |
| EcoT38I | GRGCYC | 1 cut(s) | 249 |
| ErhI | CCWWGG | 3 cut(s) | 314, 772, 1337 |
| FaeI | CATG | 6 cut(s) | 257, 1202, 1265, 1341, 1353, 1574 |
| FaqI | GGGAC | 2 cut(s) | 196, 832 |
| FatI | CATG | 6 cut(s) | 253, 1198, 1261, 1337, 1349, 1570 |
| FauNDI | CATATG | 1 cut(s) | 811 |
| Fnu4HI | GCNGC | 6 cut(s) | 360, 440, 588, 678, 1110, 1494 |
| FokI | GGATG | 2 cut(s) | 17, 1434 |
| FriOI | GRGCYC | 1 cut(s) | 249 |
| Fsp4HI | GCNGC | 6 cut(s) | 360, 440, 588, 678, 1110, 1494 |
| FspBI | CTAG | 4 cut(s) | 59, 137, 1322, 1362 |
| GlaI | GCGC | 1 cut(s) | 1370 |
| GluI | GCNGC | 6 cut(s) | 360, 440, 588, 678, 1110, 1494 |
| GsaI | CCCAGC | 1 cut(s) | 74 |
| GsuI | CTGGAG | 2 cut(s) | 898, 995 |
| HaeIII | GGCC | 2 cut(s) | 652, 1553 |
| HapII | CCGG | 1 cut(s) | 504 |
| HgaI | GACGC | 2 cut(s) | 423, 1360 |
| HhaI | GCGC | 1 cut(s) | 1371 |
| Hin1I | GRCGYC | 1 cut(s) | 434 |
| Hin1II | CATG | 6 cut(s) | 257, 1202, 1265, 1341, 1353, 1574 |
| Hin6I | GCGC | 1 cut(s) | 1369 |
| HinP1I | GCGC | 1 cut(s) | 1369 |
| HincII | GTYRAC | 3 cut(s) | 219, 877, 1129 |
| HindII | GTYRAC | 3 cut(s) | 219, 877, 1129 |
| HindIII | AAGCTT | 3 cut(s) | 404, 968, 1520 |
| HinfI | GANTC | 6 cut(s) | 27, 184, 385, 890, 1125, 1148 |
| HpaI | GTTAAC | 2 cut(s) | 219, 877 |
| HpaII | CCGG | 1 cut(s) | 504 |
| HphI | GGTGA | 3 cut(s) | 37, 283, 566 |
| Hpy166II | GTNNAC | 7 cut(s) | 219, 499, 501, 877, 910, 1129, 1332 |
| Hpy188I | TCNGA | 6 cut(s) | 91, 106, 205, 790, 1012, 1124 |
| Hpy188III | TCNNGA | 7 cut(s) | 24, 242, 604, 964, 974, 986, 1375 |
| Hpy8I | GTNNAC | 7 cut(s) | 219, 499, 501, 877, 910, 1129, 1332 |
| Hpy99I | CGWCG | 2 cut(s) | 439, 1376 |
| HpyAV | CCTTC | 4 cut(s) | 52, 59, 809, 1537 |
| HpyCH4III | ACNGT | 3 cut(s) | 907, 1102, 1258 |
| HpyCH4V | TGCA | 9 cut(s) | 362, 413, 695, 809, 947, 1198, 1344, 1439, 1493 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 329, 359, 683, 692, 976 |
| HpyF3I | CTNAG | 7 cut(s) | 333, 354, 371, 402, 789, 1186, 1386 |
| Hsp92I | GRCGYC | 1 cut(s) | 434 |
| Hsp92II | CATG | 6 cut(s) | 257, 1202, 1265, 1341, 1353, 1574 |
| HspAI | GCGC | 1 cut(s) | 1369 |
| KspAI | GTTAAC | 2 cut(s) | 219, 877 |
| Kzo9I | GATC | 2 cut(s) | 447, 1515 |
| LguI | GCTCTTC | 1 cut(s) | 165 |
| LmnI | GCTCC | 3 cut(s) | 316, 832, 976 |
| Lsp1109I | GCAGC | 5 cut(s) | 346, 426, 689, 1096, 1505 |
| LweI | GCATC | 4 cut(s) | 400, 619, 1353, 1420 |
| MaeI | CTAG | 4 cut(s) | 59, 137, 1322, 1362 |
| MaeIII | GTNAC | 1 cut(s) | 132 |
| MalI | GATC | 2 cut(s) | 449, 1517 |
| MbiI | CCGCTC | 1 cut(s) | 418 |
| MboI | GATC | 2 cut(s) | 447, 1515 |
| MhlI | GDGCHC | 2 cut(s) | 249, 1100 |
| MlsI | TGGCCA | 1 cut(s) | 1553 |
| MluNI | TGGCCA | 1 cut(s) | 1553 |
| MlyI | GAGTC | 4 cut(s) | 178, 394, 1134, 1157 |
| MmeI | TCCRAC | 3 cut(s) | 1181, 1252, 1499 |
| Mox20I | TGGCCA | 1 cut(s) | 1553 |
| MroXI | GAANNNNTTC | 1 cut(s) | 597 |
| MscI | TGGCCA | 1 cut(s) | 1553 |
| MseI | TTAA | 4 cut(s) | 218, 876, 924, 1067 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1103, 1311, 1339 |
| Msp20I | TGGCCA | 1 cut(s) | 1553 |
| MspA1I | CMGCKG | 2 cut(s) | 587, 686 |
| MspI | CCGG | 1 cut(s) | 504 |
| MspR9I | CCNGG | 1 cut(s) | 1550 |
| MvaI | CCWGG | 1 cut(s) | 1550 |
| MvnI | CGCG | 1 cut(s) | 1371 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 329, 359, 683, 692, 976 |
| NcoI | CCATGG | 1 cut(s) | 1337 |
| NdeI | CATATG | 1 cut(s) | 811 |
| NdeII | GATC | 2 cut(s) | 447, 1515 |
| NlaIII | CATG | 6 cut(s) | 257, 1202, 1265, 1341, 1353, 1574 |
| NlaIV | GGNNCC | 5 cut(s) | 458, 487, 582, 651, 978 |
| NspI | RCATGY | 2 cut(s) | 1353, 1574 |
| OliI | CACNNNNGTG | 2 cut(s) | 1103, 1339 |
| PciI | ACATGT | 2 cut(s) | 1349, 1570 |
| PciSI | GCTCTTC | 1 cut(s) | 165 |
| PdmI | GAANNNNTTC | 1 cut(s) | 597 |
| PfeI | GAWTC | 2 cut(s) | 27, 890 |
| PinAI | ACCGGT | 1 cut(s) | 503 |
| PkrI | GCNGC | 6 cut(s) | 361, 441, 589, 679, 1111, 1495 |
| PleI | GAGTC | 4 cut(s) | 178, 393, 1133, 1156 |
| PpsI | GAGTC | 4 cut(s) | 178, 393, 1133, 1156 |
| PpuMI | RGGWCCY | 1 cut(s) | 1546 |
| PscI | ACATGT | 2 cut(s) | 1349, 1570 |
| Psp124BI | GAGCTC | 1 cut(s) | 249 |
| Psp5II | RGGWCCY | 1 cut(s) | 1546 |
| Psp6I | CCWGG | 1 cut(s) | 1548 |
| PspFI | CCCAGC | 1 cut(s) | 70 |
| PspGI | CCWGG | 1 cut(s) | 1548 |
| PspN4I | GGNNCC | 5 cut(s) | 458, 487, 582, 651, 978 |
| PspPI | GGNCC | 2 cut(s) | 650, 1546 |
| PspPPI | RGGWCCY | 1 cut(s) | 1546 |
| PstNI | CAGNNNCTG | 1 cut(s) | 89 |
| PvuII | CAGCTG | 1 cut(s) | 686 |
| RsaI | GTAC | 1 cut(s) | 500 |
| RsaNI | GTAC | 1 cut(s) | 499 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1103, 1311, 1339 |
| SacI | GAGCTC | 1 cut(s) | 249 |
| SapI | GCTCTTC | 1 cut(s) | 165 |
| SaqAI | TTAA | 4 cut(s) | 218, 876, 924, 1067 |
| SatI | GCNGC | 6 cut(s) | 360, 440, 588, 678, 1110, 1494 |
| Sau3AI | GATC | 2 cut(s) | 447, 1515 |
| Sau96I | GGNCC | 2 cut(s) | 650, 1546 |
| SchI | GAGTC | 4 cut(s) | 178, 394, 1134, 1157 |
| ScrFI | CCNGG | 1 cut(s) | 1550 |
| SduI | GDGCHC | 2 cut(s) | 249, 1100 |
| SfaNI | GCATC | 4 cut(s) | 400, 619, 1353, 1420 |
| SfcI | CTRYAG | 1 cut(s) | 1607 |
| SgrAI | CRCCGGYG | 1 cut(s) | 503 |
| SinI | GGWCC | 1 cut(s) | 1546 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1103, 1311, 1339 |
| SmlI | CTYRAG | 4 cut(s) | 242, 443, 492, 602 |
| SmoI | CTYRAG | 4 cut(s) | 242, 443, 492, 602 |
| SpeI | ACTAGT | 1 cut(s) | 58 |
| SsiI | CCGC | 3 cut(s) | 418, 518, 587 |
| SspI | AATATT | 1 cut(s) | 840 |
| SspMI | CTAG | 4 cut(s) | 59, 137, 1322, 1362 |
| SstI | GAGCTC | 1 cut(s) | 249 |
| StyD4I | CCNGG | 1 cut(s) | 1548 |
| StyI | CCWWGG | 3 cut(s) | 314, 772, 1337 |
| TaaI | ACNGT | 3 cut(s) | 907, 1102, 1258 |
| TaqI | TCGA | 3 cut(s) | 723, 997, 1374 |
| TatI | WGTACW | 1 cut(s) | 498 |
| TauI | GCSGC | 1 cut(s) | 590 |
| TfiI | GAWTC | 2 cut(s) | 27, 890 |
| Tru1I | TTAA | 4 cut(s) | 218, 876, 924, 1067 |
| Tru9I | TTAA | 4 cut(s) | 218, 876, 924, 1067 |
| TscAI | CASTG | 3 cut(s) | 379, 811, 1105 |
| TseI | GCWGC | 5 cut(s) | 359, 439, 677, 1109, 1493 |
| TspRI | CASTG | 3 cut(s) | 379, 811, 1105 |
| VpaK11BI | GGWCC | 1 cut(s) | 1546 |
| XapI | RAATTY | 6 cut(s) | 596, 640, 665, 895, 959, 1399 |
| XceI | RCATGY | 2 cut(s) | 1353, 1574 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1268 |
| XmnI | GAANNNNTTC | 1 cut(s) | 597 |
| XspI | CTAG | 4 cut(s) | 59, 137, 1322, 1362 |