RLG00000001566

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000193
Physical Location & Seq
Reverse (-)
17586816 .. 17589515
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001566

Sequence Viewer

Length: 729 bp
ATGGCGTTGGACCTTAAAACTCAACTGGGTTATTTCAAGAATGTGGAGAAGCAGTTGAGGCACAGACTAGGTGAAGCAGAAGCTCACACATTGTTGTCCCAAGCTGTTTACTTGATTGCCATCGGAAGCAATGATTACGTTGCATTCATAACGAATTCAAGTTTGTTCGAGTCTCGTTCACATGAAGAAAATGTTGGCATGGTGACAGGAAACATTACAAATGTGATCAAAGAAATATACAAGAAAGGAGGAAGAAAATTTGGGATTAAAGGCATAGAGAATTTGGGTTGTATCCCGGGCTTGAGAACAGTTAAACCAGGAAACACAAGCACCTGTAATGAAGAAGTAAATGCAATTTCAAAACTCCACGATAGAGTACTTGCTAAAGTCCTCCTGGAGCTGAAAGGACAGCTCCAAGACTTCATATACTCGAATCCAAATTTCTACTCTTACGCAAATGACGTAGTTAATAATCCATCAAAACATGGTTTCAAGGAAGGAAAGATGGCATGCTGTGGCTCTGGTCCATACAGAGGAATTAGTAGCTGCGGAGGGAAGCGAGGTGGTGTGACTGAGTATCAGTTATGTGACAATGTTACTGACTACGTCTACTTTGATTCCGGCCATCCCACAGAAAGGTATAACCAGAAACTTTCCAAGTTATGGTGGAGCCAGACTCCCGATGTCACAGCACGTTACATCAATTTGAAAGAGCTATTTGAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

243

Amino Acids

27.06

Weight (kDa)

8.62

Isoelectric Point (pI)

26.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 221 1.1e-11 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 663
AccI GTMKAC 1 cut(s) 609
AciI CCGC 1 cut(s) 549
AcoI YGGCCR 1 cut(s) 622
AcsI RAATTY 4 cut(s) 154, 257, 280, 439
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 3 cut(s) 533, 636, 663
AgsI TTSAA 6 cut(s) 37, 159, 360, 493, 709, 722
AjnI CCWGG 2 cut(s) 316, 393
AjuI GAANNNNNNNTTGG 2 cut(s) 177, 209
AluBI AGCT 6 cut(s) 83, 104, 400, 412, 546, 715
AluI AGCT 6 cut(s) 83, 104, 400, 412, 546, 715
Alw26I GTCTC 1 cut(s) 177
Ama87I CYCGRG 1 cut(s) 295
AoxI GGCC 1 cut(s) 622
ApeKI GCWGC 1 cut(s) 546
ApoI RAATTY 4 cut(s) 154, 257, 280, 439
AspS9I GGNCC 2 cut(s) 10, 524
AsuC2I CCSGG 2 cut(s) 296, 297
AsuHPI GGTGA 2 cut(s) 83, 214
AvaI CYCGRG 1 cut(s) 295
AvaII GGWCC 2 cut(s) 10, 524
BbvI GCAGC 1 cut(s) 533
BccI CCATC 4 cut(s) 128, 484, 499, 633
BciT130I CCWGG 2 cut(s) 318, 395
BciVI GTATCC 1 cut(s) 302
BclI TGATCA 1 cut(s) 225
BcnI CCSGG 2 cut(s) 296, 297
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 1 cut(s) 68
BfuI GTATCC 1 cut(s) 302
BisI GCNGC 1 cut(s) 547
BlsI GCNGC 1 cut(s) 548
BmcAI AGTACT 1 cut(s) 378
Bme1390I CCNGG 4 cut(s) 296, 297, 318, 395
Bme18I GGWCC 2 cut(s) 10, 524
BmeT110I CYCGRG 1 cut(s) 295
BmgT120I GGNCC 2 cut(s) 10, 524
BmiI GGNNCC 1 cut(s) 671
BmrFI CCNGG 4 cut(s) 296, 297, 318, 395
BmrI ACTGGG 1 cut(s) 35
BmuI ACTGGG 1 cut(s) 35
BplI GAGNNNNNCTC 2 cut(s) 661, 693
BpmI CTGGAG 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 322
BpuMI CCSGG 2 cut(s) 296, 297
BsaBI GATNNNNATC 1 cut(s) 119
BsaJI CCNNGG 1 cut(s) 295
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 3 cut(s) 533, 636, 663
Bse1I ACTGG 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 136
Bse8I GATNNNNATC 1 cut(s) 119
BseBI CCWGG 2 cut(s) 318, 395
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 1 cut(s) 625
BseJI GATNNNNATC 1 cut(s) 119
BseLI CCNNNNNNNGG 3 cut(s) 533, 636, 663
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 1 cut(s) 564
BseNI ACTGG 1 cut(s) 30
BseXI GCAGC 1 cut(s) 533
BshFI GGCC 1 cut(s) 624
BsiHKCI CYCGRG 1 cut(s) 295
BsiSI CCGG 2 cut(s) 296, 621
BslFI GGGAC 1 cut(s) 82
BslI CCNNNNNNNGG 3 cut(s) 533, 636, 663
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 82
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 1 cut(s) 624
BsoBI CYCGRG 1 cut(s) 295
Bsp143I GATC 1 cut(s) 225
BspACI CCGC 1 cut(s) 549
BspANI GGCC 1 cut(s) 624
BspCNI CTCAG 1 cut(s) 565
BspLI GGNNCC 1 cut(s) 671
BsrDI GCAATG 1 cut(s) 136
BsrI ACTGG 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 1 cut(s) 225
Bst2UI CCWGG 2 cut(s) 318, 395
Bst4CI ACNGT 1 cut(s) 310
BstC8I GCNNGC 1 cut(s) 511
BstDEI CTNAG 1 cut(s) 573
BstF5I GGATG 1 cut(s) 625
BstKTI GATC 1 cut(s) 228
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 1 cut(s) 225
BstMWI GCNNNNNNNGC 1 cut(s) 58
BstNI CCWGG 2 cut(s) 318, 395
BstNSI RCATGY 1 cut(s) 513
BstSCI CCNGG 4 cut(s) 294, 295, 316, 393
BstV1I GCAGC 1 cut(s) 533
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 1 cut(s) 624
BtsCI GGATG 1 cut(s) 625
Cac8I GCNNGC 1 cut(s) 511
Cfr13I GGNCC 2 cut(s) 10, 524
Cfr9I CCCGGG 1 cut(s) 295
Csp6I GTAC 1 cut(s) 377
CviAII CATG 4 cut(s) 182, 199, 485, 510
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 1 cut(s) 573
DpnI GATC 1 cut(s) 227
DpnII GATC 1 cut(s) 225
EaeI YGGCCR 1 cut(s) 622
Eco47I GGWCC 2 cut(s) 10, 524
Eco88I CYCGRG 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 316, 393
FaeI CATG 4 cut(s) 185, 202, 488, 513
FaqI GGGAC 1 cut(s) 82
FatI CATG 4 cut(s) 181, 198, 484, 509
FbaI TGATCA 1 cut(s) 225
FblI GTMKAC 1 cut(s) 609
Fnu4HI GCNGC 1 cut(s) 547
FokI GGATG 1 cut(s) 612
Fsp4HI GCNGC 1 cut(s) 547
FspBI CTAG 1 cut(s) 68
GluI GCNGC 1 cut(s) 547
GsuI CTGGAG 1 cut(s) 416
HaeIII GGCC 1 cut(s) 624
HapII CCGG 2 cut(s) 296, 621
Hin1II CATG 4 cut(s) 185, 202, 488, 513
HinfI GANTC 4 cut(s) 170, 433, 617, 676
HpaII CCGG 2 cut(s) 296, 621
HphI GGTGA 2 cut(s) 83, 214
Hpy166II GTNNAC 3 cut(s) 109, 179, 610
Hpy188I TCNGA 1 cut(s) 125
Hpy188III TCNNGA 2 cut(s) 37, 680
Hpy8I GTNNAC 3 cut(s) 109, 179, 610
HpyAV CCTTC 1 cut(s) 491
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 4 cut(s) 138, 462, 606, 694
HpyCH4V TGCA 2 cut(s) 143, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 58
HpyF3I CTNAG 1 cut(s) 573
HpySE526I ACGT 4 cut(s) 138, 462, 606, 694
Hsp92II CATG 4 cut(s) 185, 202, 488, 513
Ksp22I TGATCA 1 cut(s) 225
Kzo9I GATC 1 cut(s) 225
LmnI GCTCC 3 cut(s) 397, 417, 669
Lsp1109I GCAGC 1 cut(s) 533
MaeI CTAG 1 cut(s) 68
MaeII ACGT 4 cut(s) 138, 462, 606, 694
MaeIII GTNAC 6 cut(s) 202, 568, 587, 595, 685, 695
MalI GATC 1 cut(s) 227
MboI GATC 1 cut(s) 225
MboII GAAGA 3 cut(s) 197, 264, 353
MluCI AATT 7 cut(s) 154, 257, 280, 354, 439, 537, 703
MlyI GAGTC 2 cut(s) 179, 670
MnlI CCTC 6 cut(s) 51, 242, 401, 527, 545, 554
MseI TTAA 4 cut(s) 15, 267, 312, 468
MspI CCGG 2 cut(s) 296, 621
MspR9I CCNGG 4 cut(s) 296, 297, 318, 395
Mva1269I GAATGC 1 cut(s) 143
MvaI CCWGG 2 cut(s) 318, 395
MwoI GCNNNNNNNGC 1 cut(s) 58
NciI CCSGG 2 cut(s) 296, 297
NdeII GATC 1 cut(s) 225
NlaIII CATG 4 cut(s) 185, 202, 488, 513
NlaIV GGNNCC 1 cut(s) 671
NmuCI GTSAC 4 cut(s) 202, 568, 587, 685
NspI RCATGY 1 cut(s) 513
PaeI GCATGC 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PctI GAATGC 1 cut(s) 143
PfeI GAWTC 2 cut(s) 433, 617
PflFI GACNNNGTC 1 cut(s) 605
PflMI CCANNNNNTGG 1 cut(s) 663
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 1 cut(s) 548
PleI GAGTC 2 cut(s) 178, 670
PpsI GAGTC 2 cut(s) 178, 670
Psp6I CCWGG 2 cut(s) 316, 393
PspGI CCWGG 2 cut(s) 316, 393
PspN4I GGNNCC 1 cut(s) 671
PspPI GGNCC 2 cut(s) 10, 524
PsyI GACNNNGTC 1 cut(s) 605
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SaqAI TTAA 4 cut(s) 15, 267, 312, 468
SatI GCNGC 1 cut(s) 547
Sau3AI GATC 1 cut(s) 225
Sau96I GGNCC 2 cut(s) 10, 524
ScaI AGTACT 1 cut(s) 378
SchI GAGTC 2 cut(s) 179, 670
ScrFI CCNGG 4 cut(s) 296, 297, 318, 395
SinI GGWCC 2 cut(s) 10, 524
SmaI CCCGGG 1 cut(s) 297
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
SphI GCATGC 1 cut(s) 513
Sse9I AATT 7 cut(s) 154, 257, 280, 354, 439, 537, 703
SsiI CCGC 1 cut(s) 549
SspMI CTAG 1 cut(s) 68
StyD4I CCNGG 4 cut(s) 294, 295, 316, 393
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 4 cut(s) 141, 465, 609, 697
TaqI TCGA 2 cut(s) 168, 431
TasI AATT 7 cut(s) 154, 257, 280, 354, 439, 537, 703
TatI WGTACW 1 cut(s) 376
TfiI GAWTC 2 cut(s) 433, 617
Tru1I TTAA 4 cut(s) 15, 267, 312, 468
Tru9I TTAA 4 cut(s) 15, 267, 312, 468
TseFI GTSAC 4 cut(s) 202, 568, 587, 685
TseI GCWGC 1 cut(s) 546
Tsp45I GTSAC 4 cut(s) 202, 568, 587, 685
TspDTI ATGAA 4 cut(s) 136, 198, 354, 412
TspMI CCCGGG 1 cut(s) 295
Tth111I GACNNNGTC 1 cut(s) 605
Van91I CCANNNNNTGG 1 cut(s) 663
VpaK11BI GGWCC 2 cut(s) 10, 524
XapI RAATTY 4 cut(s) 154, 257, 280, 439
XceI RCATGY 1 cut(s) 513
XmaI CCCGGG 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 609
XspI CTAG 1 cut(s) 68
ZrmI AGTACT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.