Rw4G003830

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000193
Physical Location & Seq
Reverse (-)
7474852 .. 7476209
1358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G003830.1

Sequence Viewer

Length: 801 bp
ATGATTGCTCAGGCCAACTACTGGCCATATGGAGAAACCTATTTCGGGTACCCCACCGGGAGATCTAGCGATGGGCGTCAAATTCCAGATTTCATCGCTGAGTATAATTTGCTATTGATTACACCATATCTACAACCCGGTTTTAATAACTATGTTTATGGAGTGAACTTTGCATCTGGGGGAGCTGGTGCTCTAAAAGAAACTCATCAAGTGATAGACCTTCAAACTCAGCTTAGTTATTTCAAAAATGTAGAAAAGCAATTGAGGCACAAACTAGGCTATGCACAAGCCAAGACATTGCTTTCTACGGCTGTCCACCTTTTCAGCATCGGAAGCAATGATTATTTCGCCCCTCTGATCAGGATGGTGATTGGCAACATAACACATGTGGTCAAAGAAATATACAAGAAAGGAGGAAGGAAATTTGGGTTTTTAAGCTTGGGACCAATTGGTTGTCATCCAGCTTTGCTAGCATTTCGGCCAGAAAAAATAGGATTTTGCATGGAAGAACTCACAGAAGAAGCACAAATACACAATAAGGCAATTTTGAAAGTCCTCCATGCGCTAGAGATCAAGCTGAAAGGATTCAGATATTCGTACTTCGACTTTTATACTTCCATGAGTGAAAGATTGAATAACCCAACAAAATACGGTTTCAAGGAAGGGAAGAAGGCATGCTGTGGAAGTGGTCCACACAGAGCCATTAACTGTGGAGGACAGGGACCAGAGAAAGAGTATGAACTATGTGACAATCCTAAGGACTGTGTTCTTCGATGGCGGTCATCCTACAGAAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

266

Amino Acids

30.11

Weight (kDa)

9.16

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 13 - 232 2.6e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 48
AccB1I GGYRCC 1 cut(s) 48
AccB7I CCANNNNNTGG 1 cut(s) 21
AciI CCGC 1 cut(s) 778
AcoI YGGCCR 2 cut(s) 23, 479
AcsI RAATTY 2 cut(s) 81, 422
AcyI GRCGYC 1 cut(s) 76
AfaI GTAC 2 cut(s) 50, 599
AfiI CCNNNNNNNGG 2 cut(s) 21, 45
AflIII ACRYGT 1 cut(s) 385
AgsI TTSAA 5 cut(s) 224, 244, 550, 634, 658
AluBI AGCT 5 cut(s) 185, 232, 438, 464, 577
AluI AGCT 5 cut(s) 185, 232, 438, 464, 577
Alw21I GWGCWC 1 cut(s) 193
AoxI GGCC 3 cut(s) 12, 23, 479
ApoI RAATTY 2 cut(s) 81, 422
Asp700I GAANNNNTTC 1 cut(s) 584
Asp718I GGTACC 1 cut(s) 48
AspLEI GCGC 1 cut(s) 565
AspS9I GGNCC 3 cut(s) 443, 689, 722
AsuC2I CCSGG 2 cut(s) 58, 138
AsuHPI GGTGA 1 cut(s) 379
AsuNHI GCTAGC 1 cut(s) 469
AvaII GGWCC 3 cut(s) 443, 689, 722
AxyI CCTNAGG 1 cut(s) 756
BalI TGGCCA 1 cut(s) 25
BanI GGYRCC 1 cut(s) 48
BarI GAAGNNNNNNTAC 2 cut(s) 513, 545
Bbv12I GWGCWC 1 cut(s) 193
BccI CCATC 3 cut(s) 65, 358, 768
BceAI ACGGC 1 cut(s) 324
BclI TGATCA 1 cut(s) 357
BcnI CCSGG 2 cut(s) 58, 138
BfaI CTAG 4 cut(s) 66, 275, 470, 566
BfmI CTRYAG 1 cut(s) 787
BglII AGATCT 1 cut(s) 62
Bme1390I CCNGG 2 cut(s) 58, 138
Bme18I GGWCC 3 cut(s) 443, 689, 722
BmgT120I GGNCC 3 cut(s) 443, 689, 722
BmiI GGNNCC 3 cut(s) 50, 444, 723
BmrFI CCNGG 2 cut(s) 58, 138
BmsI GCATC 2 cut(s) 182, 336
BmtI GCTAGC 1 cut(s) 473
Bpu10I CCTNAGC 1 cut(s) 9
BpuMI CCSGG 2 cut(s) 58, 138
BsaHI GRCGYC 1 cut(s) 76
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 45
Bse1I ACTGG 1 cut(s) 26
Bse21I CCTNAGG 1 cut(s) 756
Bse3DI GCAATG 2 cut(s) 296, 343
BseGI GGATG 3 cut(s) 369, 457, 782
BseLI CCNNNNNNNGG 2 cut(s) 21, 45
BseMI GCAATG 2 cut(s) 296, 343
BseMII CTCAG 3 cut(s) 23, 90, 242
BseNI ACTGG 1 cut(s) 26
BshFI GGCC 3 cut(s) 14, 25, 481
BshNI GGYRCC 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 2 cut(s) 57, 138
BslFI GGGAC 2 cut(s) 456, 735
BslI CCNNNNNNNGG 2 cut(s) 21, 45
BsmFI GGGAC 2 cut(s) 456, 735
BsnI GGCC 3 cut(s) 14, 25, 481
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 3 cut(s) 62, 357, 570
BspACI CCGC 1 cut(s) 778
BspANI GGCC 3 cut(s) 14, 25, 481
BspCNI CTCAG 3 cut(s) 22, 91, 241
BspLI GGNNCC 3 cut(s) 50, 444, 723
BspOI GCTAGC 1 cut(s) 473
BspT107I GGYRCC 1 cut(s) 48
BsrDI GCAATG 2 cut(s) 296, 343
BsrI ACTGG 1 cut(s) 26
BssMI GATC 3 cut(s) 62, 357, 570
BssNI GRCGYC 1 cut(s) 76
Bst4CI ACNGT 3 cut(s) 653, 710, 764
BstACI GRCGYC 1 cut(s) 76
BstC8I GCNNGC 2 cut(s) 471, 676
BstDEI CTNAG 5 cut(s) 9, 99, 228, 233, 756
BstF5I GGATG 3 cut(s) 369, 457, 782
BstHHI GCGC 1 cut(s) 565
BstKTI GATC 3 cut(s) 65, 360, 573
BstMBI GATC 3 cut(s) 62, 357, 570
BstMWI GCNNNNNNNGC 3 cut(s) 265, 333, 470
BstNSI RCATGY 2 cut(s) 389, 678
BstSCI CCNGG 2 cut(s) 56, 136
BstSFI CTRYAG 1 cut(s) 787
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
Bsu36I CCTNAGG 1 cut(s) 756
BsuRI GGCC 3 cut(s) 14, 25, 481
BtgZI GCGATG 2 cut(s) 79, 84
BtsCI GGATG 3 cut(s) 369, 457, 782
Cac8I GCNNGC 2 cut(s) 471, 676
CfoI GCGC 1 cut(s) 565
Cfr13I GGNCC 3 cut(s) 443, 689, 722
CseI GACGC 1 cut(s) 65
Csp6I GTAC 2 cut(s) 49, 598
CviAII CATG 5 cut(s) 386, 502, 560, 619, 675
CviQI GTAC 2 cut(s) 49, 598
DdeI CTNAG 5 cut(s) 9, 99, 228, 233, 756
DpnI GATC 3 cut(s) 64, 359, 572
DpnII GATC 3 cut(s) 62, 357, 570
EaeI YGGCCR 2 cut(s) 23, 479
Eco47I GGWCC 3 cut(s) 443, 689, 722
Eco81I CCTNAGG 1 cut(s) 756
FaeI CATG 5 cut(s) 389, 505, 563, 622, 678
FaqI GGGAC 2 cut(s) 456, 735
FatI CATG 5 cut(s) 385, 501, 559, 618, 674
FauNDI CATATG 1 cut(s) 28
FbaI TGATCA 1 cut(s) 357
FokI GGATG 3 cut(s) 376, 444, 769
FspBI CTAG 4 cut(s) 66, 275, 470, 566
GlaI GCGC 1 cut(s) 564
HaeIII GGCC 3 cut(s) 14, 25, 481
HapII CCGG 2 cut(s) 57, 138
HgaI GACGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 565
Hin1I GRCGYC 1 cut(s) 76
Hin1II CATG 5 cut(s) 389, 505, 563, 622, 678
Hin6I GCGC 1 cut(s) 563
HinP1I GCGC 1 cut(s) 563
HindIII AAGCTT 1 cut(s) 436
HinfI GANTC 1 cut(s) 585
HpaII CCGG 2 cut(s) 57, 138
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 3 cut(s) 166, 316, 692
Hpy188I TCNGA 3 cut(s) 332, 357, 590
Hpy188III TCNNGA 2 cut(s) 86, 361
Hpy8I GTNNAC 3 cut(s) 166, 316, 692
HpyAV CCTTC 4 cut(s) 230, 411, 656, 664
HpyCH4III ACNGT 3 cut(s) 653, 710, 764
HpyCH4V TGCA 3 cut(s) 173, 284, 501
HpyF10VI GCNNNNNNNGC 3 cut(s) 265, 333, 470
HpyF3I CTNAG 5 cut(s) 9, 99, 228, 233, 756
Hsp92I GRCGYC 1 cut(s) 76
Hsp92II CATG 5 cut(s) 389, 505, 563, 622, 678
HspAI GCGC 1 cut(s) 563
KpnI GGTACC 1 cut(s) 52
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 3 cut(s) 62, 357, 570
LmnI GCTCC 1 cut(s) 182
LweI GCATC 2 cut(s) 182, 336
MaeI CTAG 4 cut(s) 66, 275, 470, 566
MaeIII GTNAC 1 cut(s) 746
MalI GATC 3 cut(s) 64, 359, 572
MboI GATC 3 cut(s) 62, 357, 570
MboII GAAGA 4 cut(s) 518, 530, 679, 761
MfeI CAATTG 2 cut(s) 260, 447
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 193
MlsI TGGCCA 1 cut(s) 25
MluCI AATT 6 cut(s) 81, 106, 260, 422, 447, 543
MluNI TGGCCA 1 cut(s) 25
MnlI CCTC 5 cut(s) 258, 363, 407, 566, 707
Mox20I TGGCCA 1 cut(s) 25
MroXI GAANNNNTTC 1 cut(s) 584
MscI TGGCCA 1 cut(s) 25
MseI TTAA 3 cut(s) 144, 434, 705
Msp20I TGGCCA 1 cut(s) 25
MspI CCGG 2 cut(s) 57, 138
MspR9I CCNGG 2 cut(s) 58, 138
MunI CAATTG 2 cut(s) 260, 447
MwoI GCNNNNNNNGC 3 cut(s) 265, 333, 470
NciI CCSGG 2 cut(s) 58, 138
NdeI CATATG 1 cut(s) 28
NdeII GATC 3 cut(s) 62, 357, 570
NheI GCTAGC 1 cut(s) 469
NlaIII CATG 5 cut(s) 389, 505, 563, 622, 678
NlaIV GGNNCC 3 cut(s) 50, 444, 723
NmuCI GTSAC 1 cut(s) 746
NspI RCATGY 2 cut(s) 389, 678
PaeI GCATGC 1 cut(s) 678
PciI ACATGT 1 cut(s) 385
PdmI GAANNNNTTC 1 cut(s) 584
PfeI GAWTC 1 cut(s) 585
PflMI CCANNNNNTGG 1 cut(s) 21
PscI ACATGT 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 50, 444, 723
PspPI GGNCC 3 cut(s) 443, 689, 722
PsuI RGATCY 1 cut(s) 62
RsaI GTAC 2 cut(s) 50, 599
RsaNI GTAC 2 cut(s) 49, 598
SaqAI TTAA 3 cut(s) 144, 434, 705
Sau3AI GATC 3 cut(s) 62, 357, 570
Sau96I GGNCC 3 cut(s) 443, 689, 722
ScrFI CCNGG 2 cut(s) 58, 138
SduI GDGCHC 1 cut(s) 193
SetI ASST 8 cut(s) 41, 187, 222, 234, 321, 440, 466, 579
SfaNI GCATC 2 cut(s) 182, 336
SfcI CTRYAG 1 cut(s) 787
SinI GGWCC 3 cut(s) 443, 689, 722
SphI GCATGC 1 cut(s) 678
Sse9I AATT 6 cut(s) 81, 106, 260, 422, 447, 543
SsiI CCGC 1 cut(s) 778
SspMI CTAG 4 cut(s) 66, 275, 470, 566
StyD4I CCNGG 2 cut(s) 56, 136
TaaI ACNGT 3 cut(s) 653, 710, 764
TaqI TCGA 2 cut(s) 603, 772
TasI AATT 6 cut(s) 81, 106, 260, 422, 447, 543
TfiI GAWTC 1 cut(s) 585
Tru1I TTAA 3 cut(s) 144, 434, 705
Tru9I TTAA 3 cut(s) 144, 434, 705
TseFI GTSAC 1 cut(s) 746
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 2 cut(s) 82, 753
Van91I CCANNNNNTGG 1 cut(s) 21
VpaK11BI GGWCC 3 cut(s) 443, 689, 722
XapI RAATTY 2 cut(s) 81, 422
XceI RCATGY 2 cut(s) 389, 678
XmnI GAANNNNTTC 1 cut(s) 584
XspI CTAG 4 cut(s) 66, 275, 470, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.