Rh2BG468900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000193
Physical Location & Seq
Reverse (-)
66200725 .. 66205143
4419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG468900.1

Sequence Viewer

Length: 1098 bp
ATGGCAGCTTTGAAGTTCCATGTTCTTATCTTGGCCTTCTGGGCAACTCTTCTTATTCCTTGTAGCTCCCTTGGCGATTCAGGGCTTAAGAAAAGACATGCAGCCCTGTACGTGTTTGGTGATTCATTATTTGACCCTGGAAACAATAACTACATAAGTACCACCAATGATTACAAGGCAGATTGGTCCCCTTACGGTGAAACTTTCTTCAAGAACCCAACTGGCAGATTTTGCGACGGACGTCTAATCCCAGATTTCATTGCTGAGTATGCAAAGTTGCCAATTGTACCGGCATTTTATCCGAGGATTGTGGACTATACTTATGGGGTCAACTTTGCTTCTGGTGGGGCTGGTGCTCTGAATGAAACATATAAAGGATTCGTGATACCCCTTAATTATCAACTGCATAATTTCAAGAATATGGAGAAGCATTTGAGGCACAATCTAGGTGATGCAAAATCCTACAAATTGCTGTCAAATGCTGTTTACTTGATCAGCATTGGAGGCAATGACTACTTCGCTCCATTCGCGACAAATTGTAGTTTCTTTGAGACTCACTCACAAGAGGAATATGTTGGCATTGTGATTGGAAACCTCACAAATGTGATCAAAGAAATATACAATAAAGGAGGACGGAAATTCGGTTTTACAAAATTGCCACCTCTGGGTCTTGTGCCATCCCAGAGACCGGGAAACAAAGGCACCTGTGTTAAAGAAGTTACATCGCTAGCAAACATACACAATGCAGTACTTCCTAAACACCTTGAAAAGCTGAAGAGCAAGCTGCAAGGATTCAAATATTCAATTGCAAAATTAGACACTTACCTAACTGAAAGAATCAATAAACCATCTAAATATGGTTTCAAGGAAGGAAAGAGTGCATGCTGTGGCTCTGGTCCATATGGAGGAATTTATAGCTGTGGAGGGAAGAGAGGTGTGACCAAGTATCAGTTATGTGCCAATGTTAGTGAGTATGTCTTTTTCGACTCTTTCCATCCAACAGAAAGGGTTTACCAGCAACTTTCCAAGCTGTGGTGGAACCAAGTACCTAATACCAAAGAGCCTTACAGTAATCTGAAAGAGCTATTTGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

40.93

Weight (kDa)

9.17

Isoelectric Point (pI)

27.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 344 5.5e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 701
AccB7I CCANNNNNTGG 1 cut(s) 1032
AccII CGCG 1 cut(s) 530
AcsI RAATTY 2 cut(s) 638, 909
AcuI CTGAAG 1 cut(s) 794
AcyI GRCGYC 1 cut(s) 241
AfaI GTAC 5 cut(s) 110, 160, 288, 750, 1047
AfiI CCNNNNNNNGG 4 cut(s) 665, 688, 905, 1032
AflII CTTAAG 1 cut(s) 86
AflIII ACRYGT 1 cut(s) 111
AgsI TTSAA 8 cut(s) 13, 211, 415, 767, 796, 804, 865, 1091
AjnI CCWGG 1 cut(s) 136
AleI CACNNNNGTG 1 cut(s) 602
AluBI AGCT 7 cut(s) 8, 66, 772, 784, 918, 1030, 1084
AluI AGCT 7 cut(s) 8, 66, 772, 784, 918, 1030, 1084
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 2 cut(s) 545, 679
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 3 cut(s) 5, 101, 784
ApoI RAATTY 2 cut(s) 638, 909
AspS9I GGNCC 2 cut(s) 186, 896
AsuC2I CCSGG 1 cut(s) 690
AsuHPI GGTGA 3 cut(s) 131, 209, 461
AsuNHI GCTAGC 1 cut(s) 727
AvaII GGWCC 2 cut(s) 186, 896
BaeI ACNNNNGTAYC 2 cut(s) 142, 175
BanI GGYRCC 1 cut(s) 701
Bbv12I GWGCWC 1 cut(s) 358
BbvI GCAGC 3 cut(s) 17, 113, 771
BccI CCATC 3 cut(s) 685, 856, 1002
BciT130I CCWGG 1 cut(s) 138
BclI TGATCA 2 cut(s) 492, 606
BcnI CCSGG 1 cut(s) 690
BcoDI GTCTC 2 cut(s) 545, 679
BfaI CTAG 2 cut(s) 446, 728
BfrI CTTAAG 1 cut(s) 86
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 3 cut(s) 6, 102, 785
BlsI GCNGC 3 cut(s) 7, 103, 786
BmcAI AGTACT 1 cut(s) 750
Bme1390I CCNGG 2 cut(s) 138, 690
Bme18I GGWCC 2 cut(s) 186, 896
BmgT120I GGNCC 2 cut(s) 186, 896
BmiI GGNNCC 3 cut(s) 188, 703, 1040
BmrFI CCNGG 2 cut(s) 138, 690
BmsI GCATC 1 cut(s) 442
BmtI GCTAGC 1 cut(s) 731
BoxI GACNNNNGTC 1 cut(s) 240
BplI GAGNNNNNCTC 2 cut(s) 542, 574
BpuMI CCSGG 1 cut(s) 690
BsaAI YACGTR 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 679
BsaJI CCNNGG 3 cut(s) 70, 136, 302
Bsc4I CCNNNNNNNGG 4 cut(s) 665, 688, 905, 1032
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 226
Bse3DI GCAATG 2 cut(s) 258, 514
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 70, 136, 302
BseGI GGATG 2 cut(s) 677, 994
BseLI CCNNNNNNNGG 4 cut(s) 665, 688, 905, 1032
BseMI GCAATG 2 cut(s) 258, 514
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 1 cut(s) 226
BseXI GCAGC 3 cut(s) 17, 113, 771
Bsh1236I CGCG 1 cut(s) 530
BshFI GGCC 1 cut(s) 35
BshNI GGYRCC 1 cut(s) 701
BsiHKAI GWGCWC 1 cut(s) 358
BsiSI CCGG 2 cut(s) 290, 689
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 4 cut(s) 665, 688, 905, 1032
BsmAI GTCTC 2 cut(s) 545, 679
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 1 cut(s) 35
Bso31I GGTCTC 1 cut(s) 679
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 2 cut(s) 492, 606
Bsp68I TCGCGA 1 cut(s) 530
BspANI GGCC 1 cut(s) 35
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 1 cut(s) 530
BspLI GGNNCC 3 cut(s) 188, 703, 1040
BspOI GCTAGC 1 cut(s) 731
BspQI GCTCTTC 1 cut(s) 770
BspT107I GGYRCC 1 cut(s) 701
BspTI CTTAAG 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 679
BsrDI GCAATG 2 cut(s) 258, 514
BsrFI RCCGGY 1 cut(s) 289
BsrI ACTGG 1 cut(s) 226
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 3 cut(s) 70, 136, 302
BssMI GATC 2 cut(s) 492, 606
BssNI GRCGYC 1 cut(s) 241
BssT1I CCWWGG 1 cut(s) 70
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 2 cut(s) 197, 1070
Bst6I CTCTTC 3 cut(s) 54, 770, 923
BstACI GRCGYC 1 cut(s) 241
BstAFI CTTAAG 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 112
BstC8I GCNNGC 3 cut(s) 729, 782, 883
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 2 cut(s) 677, 994
BstFNI CGCG 1 cut(s) 530
BstKTI GATC 2 cut(s) 495, 609
BstMAI GTCTC 2 cut(s) 545, 679
BstMBI GATC 2 cut(s) 492, 606
BstMWI GCNNNNNNNGC 7 cut(s) 41, 72, 231, 269, 436, 504, 527
BstNI CCWGG 1 cut(s) 138
BstNSI RCATGY 2 cut(s) 101, 885
BstPAI GACNNNNGTC 1 cut(s) 240
BstSCI CCNGG 2 cut(s) 136, 688
BstUI CGCG 1 cut(s) 530
BstV1I GCAGC 3 cut(s) 17, 113, 771
BsuRI GGCC 1 cut(s) 35
BtgZI GCGATG 1 cut(s) 708
BtsCI GGATG 2 cut(s) 677, 994
BtuMI TCGCGA 1 cut(s) 530
Cac8I GCNNGC 3 cut(s) 729, 782, 883
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 2 cut(s) 186, 896
Csp6I GTAC 5 cut(s) 109, 159, 287, 749, 1046
CviAII CATG 3 cut(s) 20, 98, 882
CviQI GTAC 5 cut(s) 109, 159, 287, 749, 1046
DdeI CTNAG 1 cut(s) 264
DpnI GATC 2 cut(s) 494, 608
DpnII GATC 2 cut(s) 492, 606
Eam1104I CTCTTC 3 cut(s) 54, 770, 923
EarI CTCTTC 3 cut(s) 54, 770, 923
Eco130I CCWWGG 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 679
Eco47I GGWCC 2 cut(s) 186, 896
Eco57I CTGAAG 1 cut(s) 794
EcoRII CCWGG 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 3 cut(s) 23, 101, 885
FaqI GGGAC 1 cut(s) 172
FatI CATG 3 cut(s) 19, 97, 881
FauNDI CATATG 1 cut(s) 901
FbaI TGATCA 2 cut(s) 492, 606
Fnu4HI GCNGC 3 cut(s) 6, 102, 785
FokI GGATG 2 cut(s) 664, 981
Fsp4HI GCNGC 3 cut(s) 6, 102, 785
FspBI CTAG 2 cut(s) 446, 728
GluI GCNGC 3 cut(s) 6, 102, 785
HaeIII GGCC 1 cut(s) 35
HapII CCGG 2 cut(s) 290, 689
Hin1I GRCGYC 1 cut(s) 241
Hin1II CATG 3 cut(s) 23, 101, 885
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 7 cut(s) 77, 122, 378, 553, 792, 837, 986
HpaII CCGG 2 cut(s) 290, 689
HphI GGTGA 3 cut(s) 131, 209, 461
Hpy166II GTNNAC 4 cut(s) 313, 331, 487, 1012
Hpy188I TCNGA 3 cut(s) 303, 360, 1077
Hpy188III TCNNGA 4 cut(s) 211, 382, 415, 529
Hpy8I GTNNAC 4 cut(s) 313, 331, 487, 1012
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 46, 863
HpyCH4III ACNGT 2 cut(s) 197, 1070
HpyCH4IV ACGT 2 cut(s) 111, 241
HpyCH4V TGCA 8 cut(s) 101, 272, 406, 455, 746, 787, 809, 881
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 72, 231, 269, 436, 504, 527
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 2 cut(s) 111, 241
Hsp92I GRCGYC 1 cut(s) 241
Hsp92II CATG 3 cut(s) 23, 101, 885
Ksp22I TGATCA 2 cut(s) 492, 606
Kzo9I GATC 2 cut(s) 492, 606
LguI GCTCTTC 1 cut(s) 770
LmnI GCTCC 2 cut(s) 71, 526
Lsp1109I GCAGC 3 cut(s) 17, 113, 771
LweI GCATC 1 cut(s) 442
MaeI CTAG 2 cut(s) 446, 728
MaeII ACGT 2 cut(s) 111, 241
MaeIII GTNAC 2 cut(s) 718, 937
MalI GATC 2 cut(s) 494, 608
MboI GATC 2 cut(s) 492, 606
MboII GAAGA 4 cut(s) 41, 199, 787, 940
MfeI CAATTG 2 cut(s) 282, 804
MhlI GDGCHC 1 cut(s) 358
MlyI GAGTC 2 cut(s) 547, 980
MmeI TCCRAC 1 cut(s) 1022
MseI TTAA 3 cut(s) 87, 393, 711
MslI CAYNNNNRTG 1 cut(s) 602
MspCI CTTAAG 1 cut(s) 86
MspI CCGG 2 cut(s) 290, 689
MspR9I CCNGG 2 cut(s) 138, 690
MunI CAATTG 2 cut(s) 282, 804
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 1 cut(s) 530
MwoI GCNNNNNNNGC 7 cut(s) 41, 72, 231, 269, 436, 504, 527
NciI CCSGG 1 cut(s) 690
NdeI CATATG 1 cut(s) 901
NdeII GATC 2 cut(s) 492, 606
NheI GCTAGC 1 cut(s) 727
NlaIII CATG 3 cut(s) 23, 101, 885
NlaIV GGNNCC 3 cut(s) 188, 703, 1040
NmuCI GTSAC 1 cut(s) 937
NruI TCGCGA 1 cut(s) 530
NspI RCATGY 2 cut(s) 101, 885
OliI CACNNNNGTG 1 cut(s) 602
PaeI GCATGC 1 cut(s) 885
PciSI GCTCTTC 1 cut(s) 770
PfeI GAWTC 5 cut(s) 77, 122, 378, 792, 837
PflMI CCANNNNNTGG 1 cut(s) 1032
PkrI GCNGC 3 cut(s) 7, 103, 786
PleI GAGTC 2 cut(s) 547, 980
PpsI GAGTC 2 cut(s) 547, 980
Ppu21I YACGTR 1 cut(s) 112
PshAI GACNNNNGTC 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 3 cut(s) 188, 703, 1040
PspPI GGNCC 2 cut(s) 186, 896
RruI TCGCGA 1 cut(s) 530
RsaI GTAC 5 cut(s) 110, 160, 288, 750, 1047
RsaNI GTAC 5 cut(s) 109, 159, 287, 749, 1046
RseI CAYNNNNRTG 1 cut(s) 602
SapI GCTCTTC 1 cut(s) 770
SaqAI TTAA 3 cut(s) 87, 393, 711
SatI GCNGC 3 cut(s) 6, 102, 785
Sau3AI GATC 2 cut(s) 492, 606
Sau96I GGNCC 2 cut(s) 186, 896
ScaI AGTACT 1 cut(s) 750
SchI GAGTC 2 cut(s) 547, 980
ScrFI CCNGG 2 cut(s) 138, 690
SduI GDGCHC 1 cut(s) 358
SfaNI GCATC 1 cut(s) 442
SinI GGWCC 2 cut(s) 186, 896
SmiMI CAYNNNNRTG 1 cut(s) 602
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SphI GCATGC 1 cut(s) 885
SspI AATATT 1 cut(s) 800
SspMI CTAG 2 cut(s) 446, 728
StyD4I CCNGG 2 cut(s) 136, 688
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 2 cut(s) 197, 1070
TaiI ACGT 2 cut(s) 114, 244
TaqI TCGA 1 cut(s) 984
TatI WGTACW 1 cut(s) 748
TfiI GAWTC 5 cut(s) 77, 122, 378, 792, 837
Tru1I TTAA 3 cut(s) 87, 393, 711
Tru9I TTAA 3 cut(s) 87, 393, 711
TseFI GTSAC 1 cut(s) 937
TseI GCWGC 3 cut(s) 5, 101, 784
Tsp45I GTSAC 1 cut(s) 937
TspDTI ATGAA 3 cut(s) 114, 247, 378
TspGWI ACGGA 2 cut(s) 252, 649
Van91I CCANNNNNTGG 1 cut(s) 1032
Vha464I CTTAAG 1 cut(s) 86
VpaK11BI GGWCC 2 cut(s) 186, 896
XapI RAATTY 2 cut(s) 638, 909
XceI RCATGY 2 cut(s) 101, 885
XspI CTAG 2 cut(s) 446, 728
ZraI GACGTC 1 cut(s) 242
ZrmI AGTACT 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.