Rh2CG443900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000193
Physical Location & Seq
Reverse (-)
60091953 .. 60141199
49247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG443900.1

Sequence Viewer

Length: 1143 bp
ATGAGAAGTTTATACGTACAAAAAAAAAGAATGGCAACTTCTTCAAGATTTCAAGTATATGTCCTGGCTTTTTGTGCAACCCTTCTTATTCAAAGTGGCTGTTATGGCCATTCTGTGCATCAGAGAAAACATACAGCCTTGTTCATCTTCGGGGATTCAACATTTGATGTTGGAAATAATAACTACATAAACACTTCCACTTTCTTTCAAGCAAATTTCTTCCCATATGGGGAAACCTTCTTCAGCCACCCGACTGGTAGGTTCTCCGATGGTCGTCTAATCCCAGATATCATTGCTGAATATGCAAACTTGCCAATGATTCCACCATACTTACAACCGGGGTTCGACAACTATACTAATGGGGTGAACTTTGCATCTGCTGGGGCTGGTGCTCTAGCTGAAACGTTTCAAGGATTTGTGTTGGACCTTAAAACTCAACTGGGTTATTTCAAGAATGTGGAGAAGCAGTTGAGGCACAGACTAGGTGAAGCAGAAGCTCACAGATTTTTGTCCGAAGCTGTTTACTTGATTAGCATTGGAAGCAATGATTACATTGCCCCATTCATAACGAATTCAAGTTTGTTCGAGTCTCACTCACATGAAGAATATGTTGGCATGGTCACAGGAAACCTGACAAATGTGATCAAAGAAATATACAAGAAAGGAGGAAGAAAATTTGGGATTGCAGGCATGGAGCCTTTGGGTTGTACACCGGGCATGAGAACAGATAAACCAGGAAACACAAGCACCTGTAAAGAAGAAGTAAATGCAATTTCAAAACTCCACAATAGAGTACTTGCTAAAGTCCTCCTGAAGCTGAAAGGACAGCTCCAAGATTTCATATACTCGAATCCAAATTTCTACTCTTACCTAAATGACATGGTTCATAATCCATCAAAACATGGTTTCAAGGAAGGAAAGATGGCATGCTGTGGCTCTGGTCCATACAGAGGAATTATGAGCTGCGGAGGGAAGCGAGGTGTGACTGAGTATCAGTTATGTGACAATGTTACTGACCATGTCTTTTTTGATTCCGGCCATGCAACGGAAAGGGTATACCAGAAAGTTTTCAAGTTATGGTGGAGCCATACTCCTGATGTCACAGCACGTTACATCAATTTGAAAGAGCTATTCGAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

380

Amino Acids

42.76

Weight (kDa)

8.74

Isoelectric Point (pI)

23.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 47 - 359 2.4e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1056
AciI CCGC 1 cut(s) 966
AclI AACGTT 1 cut(s) 404
AcoI YGGCCR 2 cut(s) 106, 1036
AcsI RAATTY 4 cut(s) 214, 571, 674, 856
AcuI CTGAAG 2 cut(s) 226, 833
AfaI GTAC 3 cut(s) 18, 709, 795
AfiI CCNNNNNNNGG 3 cut(s) 229, 950, 1045
AjnI CCWGG 2 cut(s) 63, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 594, 626
AluBI AGCT 7 cut(s) 398, 497, 518, 817, 829, 963, 1129
AluI AGCT 7 cut(s) 398, 497, 518, 817, 829, 963, 1129
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 1 cut(s) 594
AoxI GGCC 2 cut(s) 106, 1036
ApeKI GCWGC 1 cut(s) 963
ApoI RAATTY 4 cut(s) 214, 571, 674, 856
Asp700I GAANNNNTTC 2 cut(s) 405, 1067
AspS9I GGNCC 2 cut(s) 424, 941
AsuC2I CCSGG 2 cut(s) 339, 714
AsuHPI GGTGA 2 cut(s) 376, 497
AsuII TTCGAA 1 cut(s) 1134
AvaII GGWCC 2 cut(s) 424, 941
BalI TGGCCA 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 394
BbvI GCAGC 1 cut(s) 950
BccI CCATC 3 cut(s) 263, 901, 916
BciT130I CCWGG 2 cut(s) 65, 735
BclI TGATCA 1 cut(s) 642
BcnI CCSGG 2 cut(s) 339, 714
BcoDI GTCTC 1 cut(s) 594
BfaI CTAG 2 cut(s) 395, 482
BisI GCNGC 1 cut(s) 964
BlsI GCNGC 1 cut(s) 965
BmcAI AGTACT 1 cut(s) 795
Bme1390I CCNGG 4 cut(s) 65, 339, 714, 735
Bme18I GGWCC 2 cut(s) 424, 941
BmgT120I GGNCC 2 cut(s) 424, 941
BmiI GGNNCC 2 cut(s) 696, 1085
BmrFI CCNGG 4 cut(s) 65, 339, 714, 735
BmrI ACTGGG 1 cut(s) 449
BmsI GCATC 2 cut(s) 127, 383
BmuI ACTGGG 1 cut(s) 449
BplI GAGNNNNNCTC 4 cut(s) 578, 610, 1075, 1107
Bpu14I TTCGAA 1 cut(s) 1134
BpuMI CCSGG 2 cut(s) 339, 714
BsaAI YACGTR 1 cut(s) 16
BsaJI CCNNGG 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 950, 1045
Bse1I ACTGG 2 cut(s) 259, 444
Bse3DI GCAATG 3 cut(s) 291, 550, 552
BseBI CCWGG 2 cut(s) 65, 735
BseDI CCNNGG 1 cut(s) 338
BseLI CCNNNNNNNGG 3 cut(s) 229, 950, 1045
BseMI GCAATG 3 cut(s) 291, 550, 552
BseMII CTCAG 1 cut(s) 978
BseNI ACTGG 2 cut(s) 259, 444
BseXI GCAGC 1 cut(s) 950
BseYI CCCAGC 1 cut(s) 380
BshFI GGCC 2 cut(s) 108, 1038
BsiHKAI GWGCWC 1 cut(s) 394
BsiSI CCGG 3 cut(s) 338, 713, 1035
BslI CCNNNNNNNGG 3 cut(s) 229, 950, 1045
BsmAI GTCTC 1 cut(s) 594
BsnI GGCC 2 cut(s) 108, 1038
Bsp119I TTCGAA 1 cut(s) 1134
Bsp1286I GDGCHC 1 cut(s) 394
Bsp1407I TGTACA 1 cut(s) 707
Bsp143I GATC 1 cut(s) 642
BspACI CCGC 1 cut(s) 966
BspANI GGCC 2 cut(s) 108, 1038
BspCNI CTCAG 1 cut(s) 979
BspLI GGNNCC 2 cut(s) 696, 1085
BspT104I TTCGAA 1 cut(s) 1134
BsrDI GCAATG 3 cut(s) 291, 550, 552
BsrGI TGTACA 1 cut(s) 707
BsrI ACTGG 2 cut(s) 259, 444
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 1 cut(s) 642
BssNAI GTATAC 1 cut(s) 1057
Bst1107I GTATAC 1 cut(s) 1057
Bst2UI CCWGG 2 cut(s) 65, 735
BstAUI TGTACA 1 cut(s) 707
BstBAI YACGTR 1 cut(s) 16
BstBI TTCGAA 1 cut(s) 1134
BstC8I GCNNGC 2 cut(s) 688, 928
BstDEI CTNAG 1 cut(s) 987
BstKTI GATC 1 cut(s) 645
BstMAI GTCTC 1 cut(s) 594
BstMBI GATC 1 cut(s) 642
BstMWI GCNNNNNNNGC 5 cut(s) 74, 105, 302, 472, 540
BstNI CCWGG 2 cut(s) 65, 735
BstNSI RCATGY 1 cut(s) 930
BstSCI CCNGG 4 cut(s) 63, 337, 712, 733
BstSNI TACGTA 1 cut(s) 16
BstV1I GCAGC 1 cut(s) 950
BstXI CCANNNNNNTGG 1 cut(s) 254
BstZ17I GTATAC 1 cut(s) 1057
BsuRI GGCC 2 cut(s) 108, 1038
Cac8I GCNNGC 2 cut(s) 688, 928
Cfr13I GGNCC 2 cut(s) 424, 941
Csp6I GTAC 3 cut(s) 17, 708, 794
CviAII CATG 9 cut(s) 599, 616, 691, 718, 880, 902, 927, 1019, 1040
CviQI GTAC 3 cut(s) 17, 708, 794
DdeI CTNAG 1 cut(s) 987
DpnI GATC 1 cut(s) 644
DpnII GATC 1 cut(s) 642
EaeI YGGCCR 2 cut(s) 106, 1036
Eco105I TACGTA 1 cut(s) 16
Eco32I GATATC 1 cut(s) 289
Eco47I GGWCC 2 cut(s) 424, 941
Eco57I CTGAAG 2 cut(s) 226, 833
EcoRI GAATTC 1 cut(s) 571
EcoRII CCWGG 2 cut(s) 63, 733
EcoRV GATATC 1 cut(s) 289
FaeI CATG 9 cut(s) 602, 619, 694, 721, 883, 905, 930, 1022, 1043
FatI CATG 9 cut(s) 598, 615, 690, 717, 879, 901, 926, 1018, 1039
FauNDI CATATG 1 cut(s) 226
FbaI TGATCA 1 cut(s) 642
FblI GTMKAC 1 cut(s) 1056
Fnu4HI GCNGC 1 cut(s) 964
Fsp4HI GCNGC 1 cut(s) 964
FspBI CTAG 2 cut(s) 395, 482
GluI GCNGC 1 cut(s) 964
GsaI CCCAGC 1 cut(s) 384
HaeIII GGCC 2 cut(s) 108, 1038
HapII CCGG 3 cut(s) 338, 713, 1035
Hin1II CATG 9 cut(s) 602, 619, 694, 721, 883, 905, 930, 1022, 1043
HinfI GANTC 5 cut(s) 155, 319, 587, 850, 1031
HpaII CCGG 3 cut(s) 338, 713, 1035
HphI GGTGA 2 cut(s) 376, 497
Hpy166II GTNNAC 4 cut(s) 367, 523, 710, 1057
Hpy188I TCNGA 3 cut(s) 123, 268, 514
Hpy188III TCNNGA 4 cut(s) 45, 451, 811, 1094
Hpy8I GTNNAC 4 cut(s) 367, 523, 710, 1057
HpyAV CCTTC 3 cut(s) 92, 247, 908
HpyCH4IV ACGT 3 cut(s) 15, 404, 1108
HpyCH4V TGCA 7 cut(s) 77, 118, 305, 374, 686, 770, 1043
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 105, 302, 472, 540
HpyF3I CTNAG 1 cut(s) 987
HpySE526I ACGT 3 cut(s) 15, 404, 1108
Hsp92II CATG 9 cut(s) 602, 619, 694, 721, 883, 905, 930, 1022, 1043
Ksp22I TGATCA 1 cut(s) 642
Kzo9I GATC 1 cut(s) 642
LmnI GCTCC 3 cut(s) 694, 834, 1083
Lsp1109I GCAGC 1 cut(s) 950
LweI GCATC 2 cut(s) 127, 383
MaeI CTAG 2 cut(s) 395, 482
MaeII ACGT 3 cut(s) 15, 404, 1108
MaeIII GTNAC 6 cut(s) 619, 982, 1001, 1009, 1099, 1109
MalI GATC 1 cut(s) 644
MboI GATC 1 cut(s) 642
MboII GAAGA 7 cut(s) 33, 139, 211, 232, 614, 681, 770
MhlI GDGCHC 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 108
MluCI AATT 7 cut(s) 214, 571, 674, 771, 856, 954, 1117
MluNI TGGCCA 1 cut(s) 108
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 2 cut(s) 151, 402
MnlI CCTC 6 cut(s) 465, 659, 818, 944, 962, 971
Mox20I TGGCCA 1 cut(s) 108
MroXI GAANNNNTTC 2 cut(s) 405, 1067
MscI TGGCCA 1 cut(s) 108
MseI TTAA 1 cut(s) 429
MslI CAYNNNNRTG 1 cut(s) 597
Msp20I TGGCCA 1 cut(s) 108
MspI CCGG 3 cut(s) 338, 713, 1035
MspR9I CCNGG 4 cut(s) 65, 339, 714, 735
MvaI CCWGG 2 cut(s) 65, 735
MwoI GCNNNNNNNGC 5 cut(s) 74, 105, 302, 472, 540
NciI CCSGG 2 cut(s) 339, 714
NdeI CATATG 1 cut(s) 226
NdeII GATC 1 cut(s) 642
NlaIII CATG 9 cut(s) 602, 619, 694, 721, 883, 905, 930, 1022, 1043
NlaIV GGNNCC 2 cut(s) 696, 1085
NmuCI GTSAC 4 cut(s) 619, 982, 1001, 1099
NspI RCATGY 1 cut(s) 930
NspV TTCGAA 1 cut(s) 1134
PaeI GCATGC 1 cut(s) 930
PdmI GAANNNNTTC 2 cut(s) 405, 1067
PfeI GAWTC 4 cut(s) 155, 319, 850, 1031
PflFI GACNNNGTC 1 cut(s) 1019
PkrI GCNGC 1 cut(s) 965
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
Ppu21I YACGTR 1 cut(s) 16
Psp1406I AACGTT 1 cut(s) 404
Psp6I CCWGG 2 cut(s) 63, 733
PspFI CCCAGC 1 cut(s) 380
PspGI CCWGG 2 cut(s) 63, 733
PspN4I GGNNCC 2 cut(s) 696, 1085
PspPI GGNCC 2 cut(s) 424, 941
PsyI GACNNNGTC 1 cut(s) 1019
RsaI GTAC 3 cut(s) 18, 709, 795
RsaNI GTAC 3 cut(s) 17, 708, 794
RseI CAYNNNNRTG 1 cut(s) 597
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 1 cut(s) 964
Sau3AI GATC 1 cut(s) 642
Sau96I GGNCC 2 cut(s) 424, 941
ScaI AGTACT 1 cut(s) 795
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 4 cut(s) 65, 339, 714, 735
SduI GDGCHC 1 cut(s) 394
SfaNI GCATC 2 cut(s) 127, 383
SfuI TTCGAA 1 cut(s) 1134
SinI GGWCC 2 cut(s) 424, 941
SmiMI CAYNNNNRTG 1 cut(s) 597
SnaBI TACGTA 1 cut(s) 16
SphI GCATGC 1 cut(s) 930
Sse9I AATT 7 cut(s) 214, 571, 674, 771, 856, 954, 1117
SsiI CCGC 1 cut(s) 966
SspMI CTAG 2 cut(s) 395, 482
StyD4I CCNGG 4 cut(s) 63, 337, 712, 733
TaiI ACGT 3 cut(s) 18, 407, 1111
TaqI TCGA 4 cut(s) 345, 585, 848, 1134
TasI AATT 7 cut(s) 214, 571, 674, 771, 856, 954, 1117
TatI WGTACW 2 cut(s) 707, 793
TfiI GAWTC 4 cut(s) 155, 319, 850, 1031
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TseFI GTSAC 4 cut(s) 619, 982, 1001, 1099
TseI GCWGC 1 cut(s) 963
Tsp45I GTSAC 4 cut(s) 619, 982, 1001, 1099
TspDTI ATGAA 5 cut(s) 133, 553, 615, 829, 875
TspGWI ACGGA 1 cut(s) 1061
Tth111I GACNNNGTC 1 cut(s) 1019
VpaK11BI GGWCC 2 cut(s) 424, 941
XapI RAATTY 4 cut(s) 214, 571, 674, 856
XceI RCATGY 1 cut(s) 930
XmiI GTMKAC 1 cut(s) 1056
XmnI GAANNNNTTC 2 cut(s) 405, 1067
XspI CTAG 2 cut(s) 395, 482
ZrmI AGTACT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.