RLG00000020255

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000193
Physical Location & Seq
Reverse (-)
62909007 .. 62911853
2847 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020255

Sequence Viewer

Length: 810 bp
ATGACGAATATCCTCGCCACTGCTAGTCGATTGGAGGATCCTCCGAAGAATCACTGCCGCTCTCCACTCCGCTCAAACCCTAGGGTGTTGGACCTTAAAACTCAACTGGGTTATTTCATGAATGTGGAGAAGCAGTTGAGGCACAGATTAGGTGAAGCAGAAGCTCACACATTGTTGTCCGAAGCTGTTTACTTGATTGCCATCGGAAGCAATGATTACGTTGCCCCATTCATAACGAATTCAAGTTTGTTCGAGTCTCATTCACATGAAGAATATGTTGGCATGGTGACAGGAAACCTCACAAATGTGATCAAAGAAATATACAAGAAAGGAGGAAGAAAATTTGGGATTAAAGGCATAGGGAATTTGGGTTGTATACCGGGCTTGAGAACAGTTAAACCAGGAAACACAAGCACCTGTAATGAAGAAGTAAATGCAATTTCAAAACTCCACAATAGAGTACTTGCTAAAGTCCTCCTGGAGCTGAAAGGACAGCTCCAAGACTTCATATACTCGAATCCAAATTTATACTCTTACGCAAATGACGTAGTTAATAATCCATCAAAACATGGTTTCAAAGAGGGAAAGACGGCATGTTGCGGATCTGGTCCATACAGAGGAATTGGGAGTTGTGGCGGGAAGCGAGGTGTGACTGAGTATCAGTTATGTGAAAATGTTACTGACTACGTCTACTTTGATTCCGGCCATCCCACAGAAAGGTATAACCAGAAAGTTTCCAAGTTATGGTGGAGCCATACTCCTGATGTCACAACACGTTACATCAAGTTGAAAGAGCTATTTGAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

270

Amino Acids

30.08

Weight (kDa)

8.94

Isoelectric Point (pI)

24.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 59 - 248 3.3e-12 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 744
AccBSI CCGCTC 2 cut(s) 60, 72
AccI GTMKAC 2 cut(s) 376, 690
AciI CCGC 4 cut(s) 58, 70, 600, 636
AclWI GGATC 3 cut(s) 32, 45, 610
AcoI YGGCCR 1 cut(s) 703
AcsI RAATTY 4 cut(s) 238, 341, 364, 523
AfaI GTAC 1 cut(s) 462
AfiI CCNNNNNNNGG 3 cut(s) 617, 717, 744
AflIII ACRYGT 1 cut(s) 773
AgsI TTSAA 5 cut(s) 243, 444, 577, 790, 803
AjnI CCWGG 2 cut(s) 400, 477
AjuI GAANNNNNNNTTGG 2 cut(s) 261, 293
AleI CACNNNNGTG 1 cut(s) 305
AluBI AGCT 5 cut(s) 164, 185, 484, 496, 796
AluI AGCT 5 cut(s) 164, 185, 484, 496, 796
Alw26I GTCTC 1 cut(s) 261
AlwI GGATC 3 cut(s) 32, 45, 610
AoxI GGCC 1 cut(s) 703
ApoI RAATTY 4 cut(s) 238, 341, 364, 523
AspA2I CCTAGG 1 cut(s) 80
AspS9I GGNCC 2 cut(s) 91, 608
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 2 cut(s) 164, 298
AvaII GGWCC 2 cut(s) 91, 608
AvrII CCTAGG 1 cut(s) 80
BamHI GGATCC 1 cut(s) 37
BccI CCATC 3 cut(s) 209, 568, 714
BceAI ACGGC 1 cut(s) 606
BciT130I CCWGG 2 cut(s) 402, 479
BclI TGATCA 1 cut(s) 309
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 1 cut(s) 261
BfaI CTAG 2 cut(s) 24, 81
BisI GCNGC 1 cut(s) 58
BlnI CCTAGG 1 cut(s) 80
BlsI GCNGC 1 cut(s) 59
BmcAI AGTACT 1 cut(s) 462
Bme1390I CCNGG 3 cut(s) 381, 402, 479
Bme18I GGWCC 2 cut(s) 91, 608
BmgT120I GGNCC 2 cut(s) 91, 608
BmiI GGNNCC 2 cut(s) 39, 752
BmrFI CCNGG 3 cut(s) 381, 402, 479
BmrI ACTGGG 1 cut(s) 116
BmuI ACTGGG 1 cut(s) 116
BplI GAGNNNNNCTC 2 cut(s) 742, 774
BpmI CTGGAG 1 cut(s) 500
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 381
BsaBI GATNNNNATC 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 80
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 617, 717, 744
Bse1I ACTGG 1 cut(s) 111
Bse3DI GCAATG 1 cut(s) 217
Bse8I GATNNNNATC 1 cut(s) 200
BseBI CCWGG 2 cut(s) 402, 479
BseDI CCNNGG 1 cut(s) 80
BseGI GGATG 1 cut(s) 706
BseJI GATNNNNATC 1 cut(s) 200
BseLI CCNNNNNNNGG 3 cut(s) 617, 717, 744
BseMI GCAATG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 645
BseNI ACTGG 1 cut(s) 111
BshFI GGCC 1 cut(s) 705
BsiSI CCGG 2 cut(s) 380, 702
BslI CCNNNNNNNGG 3 cut(s) 617, 717, 744
BsmAI GTCTC 1 cut(s) 261
BsnI GGCC 1 cut(s) 705
Bsp143I GATC 3 cut(s) 37, 309, 602
BspACI CCGC 4 cut(s) 58, 70, 600, 636
BspANI GGCC 1 cut(s) 705
BspCNI CTCAG 1 cut(s) 646
BspHI TCATGA 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 39, 752
BspPI GGATC 3 cut(s) 32, 45, 610
BsrBI CCGCTC 2 cut(s) 60, 72
BsrDI GCAATG 1 cut(s) 217
BsrI ACTGG 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 3 cut(s) 37, 309, 602
BssNAI GTATAC 1 cut(s) 377
BssT1I CCWWGG 1 cut(s) 80
Bst1107I GTATAC 1 cut(s) 377
Bst2UI CCWGG 2 cut(s) 402, 479
Bst4CI ACNGT 1 cut(s) 394
BstDEI CTNAG 1 cut(s) 654
BstF5I GGATG 1 cut(s) 706
BstKTI GATC 3 cut(s) 40, 312, 605
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 3 cut(s) 37, 309, 602
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstNI CCWGG 2 cut(s) 402, 479
BstNSI RCATGY 1 cut(s) 597
BstSCI CCNGG 3 cut(s) 379, 400, 477
BstX2I RGATCY 2 cut(s) 37, 602
BstYI RGATCY 2 cut(s) 37, 602
BstZ17I GTATAC 1 cut(s) 377
BsuRI GGCC 1 cut(s) 705
BtsCI GGATG 1 cut(s) 706
BtsI GCAGTG 2 cut(s) 18, 52
BtsIMutI CAGTG 2 cut(s) 18, 52
CciI TCATGA 1 cut(s) 117
Cfr13I GGNCC 2 cut(s) 91, 608
Csp6I GTAC 1 cut(s) 461
CviAII CATG 5 cut(s) 118, 266, 283, 569, 594
CviJI RGCY 8 cut(s) 164, 185, 384, 484, 496, 705, 753, 796
CviKI_1 RGCY 8 cut(s) 164, 185, 384, 484, 496, 705, 753, 796
CviQI GTAC 1 cut(s) 461
DdeI CTNAG 1 cut(s) 654
DpnI GATC 3 cut(s) 39, 311, 604
DpnII GATC 3 cut(s) 37, 309, 602
EaeI YGGCCR 1 cut(s) 703
Eco130I CCWWGG 1 cut(s) 80
Eco47I GGWCC 2 cut(s) 91, 608
EcoRI GAATTC 1 cut(s) 238
EcoRII CCWGG 2 cut(s) 400, 477
EcoT14I CCWWGG 1 cut(s) 80
ErhI CCWWGG 1 cut(s) 80
FaeI CATG 5 cut(s) 121, 269, 286, 572, 597
FatI CATG 5 cut(s) 117, 265, 282, 568, 593
FauI CCCGC 1 cut(s) 629
FbaI TGATCA 1 cut(s) 309
FblI GTMKAC 2 cut(s) 376, 690
Fnu4HI GCNGC 1 cut(s) 58
FokI GGATG 1 cut(s) 693
Fsp4HI GCNGC 1 cut(s) 58
FspBI CTAG 2 cut(s) 24, 81
GluI GCNGC 1 cut(s) 58
GsuI CTGGAG 1 cut(s) 500
HaeIII GGCC 1 cut(s) 705
HapII CCGG 2 cut(s) 380, 702
Hin1II CATG 5 cut(s) 121, 269, 286, 572, 597
HinfI GANTC 4 cut(s) 49, 254, 517, 698
HpaII CCGG 2 cut(s) 380, 702
HphI GGTGA 2 cut(s) 164, 298
Hpy166II GTNNAC 3 cut(s) 190, 377, 691
Hpy188I TCNGA 3 cut(s) 45, 181, 206
Hpy188III TCNNGA 2 cut(s) 118, 761
Hpy8I GTNNAC 3 cut(s) 190, 377, 691
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 4 cut(s) 219, 546, 687, 775
HpyCH4V TGCA 1 cut(s) 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 1 cut(s) 654
HpySE526I ACGT 4 cut(s) 219, 546, 687, 775
Hsp92II CATG 5 cut(s) 121, 269, 286, 572, 597
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 3 cut(s) 37, 309, 602
LmnI GCTCC 3 cut(s) 481, 501, 750
MaeI CTAG 2 cut(s) 24, 81
MaeII ACGT 4 cut(s) 219, 546, 687, 775
MaeIII GTNAC 5 cut(s) 286, 649, 676, 766, 776
MalI GATC 3 cut(s) 39, 311, 604
MbiI CCGCTC 2 cut(s) 60, 72
MboI GATC 3 cut(s) 37, 309, 602
MboII GAAGA 4 cut(s) 58, 281, 348, 437
MflI RGATCY 2 cut(s) 37, 602
MluCI AATT 6 cut(s) 238, 341, 364, 438, 523, 621
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 69
MseI TTAA 4 cut(s) 96, 351, 396, 552
MslI CAYNNNNRTG 3 cut(s) 122, 264, 305
MspI CCGG 2 cut(s) 380, 702
MspR9I CCNGG 3 cut(s) 381, 402, 479
MvaI CCWGG 2 cut(s) 402, 479
MwoI GCNNNNNNNGC 1 cut(s) 139
NciI CCSGG 1 cut(s) 381
NdeII GATC 3 cut(s) 37, 309, 602
NlaIII CATG 5 cut(s) 121, 269, 286, 572, 597
NlaIV GGNNCC 2 cut(s) 39, 752
NmuCI GTSAC 3 cut(s) 286, 649, 766
NspI RCATGY 1 cut(s) 597
OliI CACNNNNGTG 1 cut(s) 305
PagI TCATGA 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PfeI GAWTC 3 cut(s) 49, 517, 698
PflFI GACNNNGTC 1 cut(s) 686
PflMI CCANNNNNTGG 1 cut(s) 744
PfoI TCCNGGA 1 cut(s) 477
PkrI GCNGC 1 cut(s) 59
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Psp6I CCWGG 2 cut(s) 400, 477
PspGI CCWGG 2 cut(s) 400, 477
PspN4I GGNNCC 2 cut(s) 39, 752
PspPI GGNCC 2 cut(s) 91, 608
PsuI RGATCY 2 cut(s) 37, 602
PsyI GACNNNGTC 1 cut(s) 686
RsaI GTAC 1 cut(s) 462
RsaNI GTAC 1 cut(s) 461
RseI CAYNNNNRTG 3 cut(s) 122, 264, 305
SaqAI TTAA 4 cut(s) 96, 351, 396, 552
SatI GCNGC 1 cut(s) 58
Sau3AI GATC 3 cut(s) 37, 309, 602
Sau96I GGNCC 2 cut(s) 91, 608
ScaI AGTACT 1 cut(s) 462
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 3 cut(s) 381, 402, 479
SinI GGWCC 2 cut(s) 91, 608
SmiMI CAYNNNNRTG 3 cut(s) 122, 264, 305
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
Sse9I AATT 6 cut(s) 238, 341, 364, 438, 523, 621
SsiI CCGC 4 cut(s) 58, 70, 600, 636
SspMI CTAG 2 cut(s) 24, 81
StyD4I CCNGG 3 cut(s) 379, 400, 477
StyI CCWWGG 1 cut(s) 80
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 4 cut(s) 222, 549, 690, 778
TaqI TCGA 3 cut(s) 28, 252, 515
TasI AATT 6 cut(s) 238, 341, 364, 438, 523, 621
TatI WGTACW 1 cut(s) 460
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 3 cut(s) 49, 517, 698
Tru1I TTAA 4 cut(s) 96, 351, 396, 552
Tru9I TTAA 4 cut(s) 96, 351, 396, 552
TscAI CASTG 2 cut(s) 25, 59
TseFI GTSAC 3 cut(s) 286, 649, 766
Tsp45I GTSAC 3 cut(s) 286, 649, 766
TspDTI ATGAA 6 cut(s) 106, 134, 220, 282, 438, 496
TspRI CASTG 2 cut(s) 25, 59
Tth111I GACNNNGTC 1 cut(s) 686
Van91I CCANNNNNTGG 1 cut(s) 744
VpaK11BI GGWCC 2 cut(s) 91, 608
XapI RAATTY 4 cut(s) 238, 341, 364, 523
XceI RCATGY 1 cut(s) 597
XmaJI CCTAGG 1 cut(s) 80
XmiI GTMKAC 2 cut(s) 376, 690
XspI CTAG 2 cut(s) 24, 81
ZrmI AGTACT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.