RLG00000020250

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000193
Physical Location & Seq
Reverse (-)
62852685 .. 62853605
921 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020250

Sequence Viewer

Length: 678 bp
ATGGAGAAGCATTTGAGGCACAATCTAGGTGATGCAAAAACCTACACATTGTTGTCAAATGCTGTTTACTTGATCAGCATTGGAGGCAATGACTATTTTGCTCCATTCGCGACAAATTGTAGTTTCTTTGAGACCGACTCACAAGAGGAATATGTTGGCATTGTGATTGGAAACCTCACAAATGTGATCAAAGAAATATACAATAAAGGAGCACGGAAATTCGGGTTTACAAGATTGCCACCTCTGGGTCTTGTGCCATCCCAGAGACCAGGAAACAAAGGCACCTGTGCTAAAGAAGTTACATCGCTAGCAAACATACACAATGCAGCACTTCCTAAACACCTAGAAAAGCTGAAAAGCAAGCTGCAAGGATTCAAATATTCAATTGCAAAATTAGACACTTACCTAACTGAAAGAATAAATAAACCATCTAAATATGGTTTCAAGGAAGGAAAGAGTGCATGCTGTGGCTCTGGTCCATATGGAGGAACTTATAGCTGTGGAGGGAAGAGAGGTGTGACCAAGTATCAGTTATGTGCCAATGTTAGTGAGTATGTCTTTTTCGACTCTTTCCATCCAACAGAAAGGGTTTACCAGCAACTTTCCGAGCTATGGTGGAACCAAGTACCTAATGCCAAAGAGCCTTACAGTAATCTGAAAGAGCTATTTGATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

226

Amino Acids

25.19

Weight (kDa)

9.08

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 19 - 204 1.1e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 281
AccII CGCG 1 cut(s) 110
AcsI RAATTY 1 cut(s) 218
AfaI GTAC 1 cut(s) 627
AfiI CCNNNNNNNGG 3 cut(s) 245, 485, 612
AgsI TTSAA 3 cut(s) 376, 384, 445
AjnI CCWGG 1 cut(s) 268
AleI CACNNNNGTG 1 cut(s) 182
AluBI AGCT 5 cut(s) 352, 364, 498, 610, 664
AluI AGCT 5 cut(s) 352, 364, 498, 610, 664
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 2 cut(s) 125, 259
ApeKI GCWGC 2 cut(s) 326, 364
ApoI RAATTY 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 476
AsuHPI GGTGA 1 cut(s) 41
AsuNHI GCTAGC 1 cut(s) 307
AvaII GGWCC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 2 cut(s) 338, 351
BccI CCATC 3 cut(s) 265, 436, 582
BciT130I CCWGG 1 cut(s) 270
BclI TGATCA 2 cut(s) 72, 186
BcoDI GTCTC 2 cut(s) 125, 259
BfaI CTAG 3 cut(s) 26, 308, 344
BisI GCNGC 2 cut(s) 327, 365
BlsI GCNGC 2 cut(s) 328, 366
Bme1390I CCNGG 1 cut(s) 270
Bme18I GGWCC 1 cut(s) 476
BmgT120I GGNCC 1 cut(s) 476
BmiI GGNNCC 2 cut(s) 283, 620
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 22
BmtI GCTAGC 1 cut(s) 311
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BsaI GGTCTC 2 cut(s) 125, 259
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 485, 612
Bse3DI GCAATG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 270
BseGI GGATG 2 cut(s) 257, 574
BseLI CCNNNNNNNGG 3 cut(s) 245, 485, 612
BseMI GCAATG 1 cut(s) 94
BseXI GCAGC 2 cut(s) 338, 351
Bsh1236I CGCG 1 cut(s) 110
BshNI GGYRCC 1 cut(s) 281
BsiHKAI GWGCWC 1 cut(s) 214
BslI CCNNNNNNNGG 3 cut(s) 245, 485, 612
BsmAI GTCTC 2 cut(s) 125, 259
Bso31I GGTCTC 2 cut(s) 125, 259
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 2 cut(s) 72, 186
Bsp68I TCGCGA 1 cut(s) 110
BspFNI CGCG 1 cut(s) 110
BspLI GGNNCC 2 cut(s) 283, 620
BspOI GCTAGC 1 cut(s) 311
BspT107I GGYRCC 1 cut(s) 281
BspTNI GGTCTC 2 cut(s) 125, 259
BsrDI GCAATG 1 cut(s) 94
BssMI GATC 2 cut(s) 72, 186
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 650
Bst6I CTCTTC 1 cut(s) 503
BstC8I GCNNGC 3 cut(s) 309, 362, 463
BstF5I GGATG 2 cut(s) 257, 574
BstFNI CGCG 1 cut(s) 110
BstKTI GATC 2 cut(s) 75, 189
BstMAI GTCTC 2 cut(s) 125, 259
BstMBI GATC 2 cut(s) 72, 186
BstMWI GCNNNNNNNGC 3 cut(s) 16, 84, 107
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 1 cut(s) 465
BstSCI CCNGG 1 cut(s) 268
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 2 cut(s) 338, 351
BtgZI GCGATG 1 cut(s) 288
BtsCI GGATG 2 cut(s) 257, 574
BtuMI TCGCGA 1 cut(s) 110
Cac8I GCNNGC 3 cut(s) 309, 362, 463
Cfr13I GGNCC 1 cut(s) 476
Csp6I GTAC 1 cut(s) 626
CviAII CATG 1 cut(s) 462
CviJI RGCY 7 cut(s) 352, 364, 471, 498, 610, 643, 664
CviKI_1 RGCY 7 cut(s) 352, 364, 471, 498, 610, 643, 664
CviQI GTAC 1 cut(s) 626
DpnI GATC 2 cut(s) 74, 188
DpnII GATC 2 cut(s) 72, 186
Eam1104I CTCTTC 1 cut(s) 503
EarI CTCTTC 1 cut(s) 503
Eco31I GGTCTC 2 cut(s) 125, 259
Eco47I GGWCC 1 cut(s) 476
EcoRII CCWGG 1 cut(s) 268
FaeI CATG 1 cut(s) 465
FatI CATG 1 cut(s) 461
FauNDI CATATG 1 cut(s) 481
FbaI TGATCA 2 cut(s) 72, 186
Fnu4HI GCNGC 2 cut(s) 327, 365
FokI GGATG 2 cut(s) 244, 561
Fsp4HI GCNGC 2 cut(s) 327, 365
FspBI CTAG 3 cut(s) 26, 308, 344
GluI GCNGC 2 cut(s) 327, 365
Hin1II CATG 1 cut(s) 465
HinfI GANTC 3 cut(s) 137, 372, 566
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 3 cut(s) 67, 228, 592
Hpy188I TCNGA 2 cut(s) 607, 657
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 3 cut(s) 67, 228, 592
HpyAV CCTTC 1 cut(s) 443
HpyCH4III ACNGT 1 cut(s) 650
HpyCH4V TGCA 5 cut(s) 35, 326, 367, 389, 461
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 84, 107
Hsp92II CATG 1 cut(s) 465
Ksp22I TGATCA 2 cut(s) 72, 186
Kzo9I GATC 2 cut(s) 72, 186
LmnI GCTCC 2 cut(s) 106, 209
LpnPI CCDG 7 cut(s) 230, 255, 275, 282, 298, 459, 608
Lsp1109I GCAGC 2 cut(s) 338, 351
LweI GCATC 1 cut(s) 22
MaeI CTAG 3 cut(s) 26, 308, 344
MaeIII GTNAC 2 cut(s) 298, 517
MalI GATC 2 cut(s) 74, 188
MboI GATC 2 cut(s) 72, 186
MboII GAAGA 1 cut(s) 520
MfeI CAATTG 1 cut(s) 384
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 4 cut(s) 115, 218, 384, 392
MlyI GAGTC 2 cut(s) 131, 560
MmeI TCCRAC 1 cut(s) 602
MnlI CCTC 8 cut(s) 9, 77, 139, 185, 252, 479, 497, 506
MslI CAYNNNNRTG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 270
MunI CAATTG 1 cut(s) 384
MvaI CCWGG 1 cut(s) 270
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 3 cut(s) 16, 84, 107
NdeI CATATG 1 cut(s) 481
NdeII GATC 2 cut(s) 72, 186
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 1 cut(s) 465
NlaIV GGNNCC 2 cut(s) 283, 620
NmuCI GTSAC 1 cut(s) 517
NruI TCGCGA 1 cut(s) 110
NspI RCATGY 1 cut(s) 465
OliI CACNNNNGTG 1 cut(s) 182
PaeI GCATGC 1 cut(s) 465
PfeI GAWTC 1 cut(s) 372
PkrI GCNGC 2 cut(s) 328, 366
PleI GAGTC 2 cut(s) 131, 560
PpsI GAGTC 2 cut(s) 131, 560
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 283, 620
PspPI GGNCC 1 cut(s) 476
RruI TCGCGA 1 cut(s) 110
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
RseI CAYNNNNRTG 1 cut(s) 182
SatI GCNGC 2 cut(s) 327, 365
Sau3AI GATC 2 cut(s) 72, 186
Sau96I GGNCC 1 cut(s) 476
SchI GAGTC 2 cut(s) 131, 560
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 1 cut(s) 22
SinI GGWCC 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 182
SphI GCATGC 1 cut(s) 465
Sse9I AATT 4 cut(s) 115, 218, 384, 392
SspI AATATT 1 cut(s) 380
SspMI CTAG 3 cut(s) 26, 308, 344
StyD4I CCNGG 1 cut(s) 268
TaaI ACNGT 1 cut(s) 650
TaqI TCGA 1 cut(s) 564
TaqII GACCGA 1 cut(s) 149
TasI AATT 4 cut(s) 115, 218, 384, 392
TfiI GAWTC 1 cut(s) 372
TseFI GTSAC 1 cut(s) 517
TseI GCWGC 2 cut(s) 326, 364
Tsp45I GTSAC 1 cut(s) 517
TspGWI ACGGA 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 476
XapI RAATTY 1 cut(s) 218
XceI RCATGY 1 cut(s) 465
XspI CTAG 3 cut(s) 26, 308, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.