Rw6G005020

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000193
Physical Location & Seq
Reverse (-)
8221076 .. 8222717
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G005020.1

Sequence Viewer

Length: 999 bp
ATGCTTCAAGGGCAAAACCGGAACTACACTGTTACTAAGAGAGTTACTGTTCATAAGCTACAGTACTTCTACTTTGTTTTAGTATATAGTAAATTCCTAGCAATTGCCTTGCTCACTTCTCTCTTCAAAAGGAGCCCACAAGCCTTTGTTTTTGGGGATTCACCATTTGATGCTAGAAACAATAAACTACATCAACAGTACGAAGACGGCTCAGGTCTTGGCCATATGGAGAAACCTATTTCGGGTATCCCACAAGAAGATACACTGATGAGCCTGACGAATTTGCCGTTATTTCCACCATATCTACAAACAGGTTTAAATAACTACGTTTATGGAGTGAAGTTTGCATCTAGGGGAGCTGGTGCTGTGATAGACATTCAAACTCAGCTTAGTTATTTCAAAAATGGAGAAAAGCAATTGAGGCACAAACTAGGCTATGCACAAGCCAAGACATTGCTTTCTAGTGCTCTGTACATTTTCAGCATTGGAAGCAATGATTACTTCGCTCCTCTGCGGTTTAACATCAGCAAAGAATTTTCATACTCACAAGAAGAATTCACAGGGATGGTGATTGGCAACTTAACACATGTGGTCAAACCAAGAAAAATAGGAACTTGCACGGTCGAACTCACAGAAAAGGCACAAATACACAATAAGGCAATTTTGAAAGTCCTCCATGCGCTAGTGATCAAGCTGAAAGGATTCAGATATTCGTACTCCGACTTCTATACTTCCATGAGTGAAAGATTGAATAACCCCACAAAATACGGTATAAGCAACATTTGGATCATCTATTGTTTTAGAAAGAGTATGAACTATATATGTGACAATCCTATTGACTATGTGTTCTTCGATGGCAGTCATCCTACAGAAAGAACTAACCAACAATTTTCCAAGCTAATGTGGAGTGAAAATTCCAATGTCACATGGCCTTGCAATGTTCAAGAACTATTTGGAACTCTTTGGGCAAAATATGTGTGTTCGACACCAAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

332

Amino Acids

38.05

Weight (kDa)

9.32

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 49 - 299 2.8e-10 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 514
AclWI GGATC 1 cut(s) 794
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 5 cut(s) 92, 280, 533, 554, 913
AfaI GTAC 4 cut(s) 65, 200, 473, 716
AfiI CCNNNNNNNGG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 586
AgsI TTSAA 7 cut(s) 8, 127, 380, 400, 667, 751, 944
AluBI AGCT 5 cut(s) 58, 359, 388, 694, 898
AluI AGCT 5 cut(s) 58, 359, 388, 694, 898
Alw21I GWGCWC 1 cut(s) 469
AlwI GGATC 1 cut(s) 794
AoxI GGCC 2 cut(s) 220, 929
ApoI RAATTY 5 cut(s) 92, 280, 533, 554, 913
Asp700I GAANNNNTTC 1 cut(s) 701
AspLEI GCGC 1 cut(s) 682
AsuHPI GGTGA 2 cut(s) 153, 580
BalI TGGCCA 1 cut(s) 222
BanII GRGCYC 1 cut(s) 137
BarI GAAGNNNNNNTAC 2 cut(s) 707, 739
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 469
BccI CCATC 2 cut(s) 559, 848
BceAI ACGGC 2 cut(s) 223, 271
BciVI GTATCC 1 cut(s) 257
BclI TGATCA 1 cut(s) 687
BfaI CTAG 6 cut(s) 98, 174, 351, 431, 462, 683
BfmI CTRYAG 2 cut(s) 59, 867
BfuI GTATCC 1 cut(s) 257
BmcAI AGTACT 1 cut(s) 65
BmiI GGNNCC 1 cut(s) 134
BmsI GCATC 2 cut(s) 160, 356
BpiI GAAGAC 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 211
BsaWI WCCGGW 1 cut(s) 18
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse3DI GCAATG 3 cut(s) 452, 499, 943
BseGI GGATG 2 cut(s) 570, 862
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMI GCAATG 3 cut(s) 452, 499, 943
BseMII CTCAG 2 cut(s) 225, 398
BseRI GAGGAG 1 cut(s) 498
Bsh1285I CGRYCG 1 cut(s) 624
BshFI GGCC 2 cut(s) 222, 931
BsiEI CGRYCG 1 cut(s) 624
BsiHKAI GWGCWC 1 cut(s) 469
BsiSI CCGG 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 242
BsnI GGCC 2 cut(s) 222, 931
Bsp1286I GDGCHC 2 cut(s) 137, 469
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 2 cut(s) 687, 786
BspACI CCGC 1 cut(s) 514
BspANI GGCC 2 cut(s) 222, 931
BspCNI CTCAG 2 cut(s) 224, 397
BspLI GGNNCC 1 cut(s) 134
BspPI GGATC 1 cut(s) 794
BsrDI GCAATG 3 cut(s) 452, 499, 943
BsrGI TGTACA 1 cut(s) 471
BssMI GATC 2 cut(s) 687, 786
Bst4CI ACNGT 6 cut(s) 31, 49, 63, 198, 622, 770
Bst6I CTCTTC 1 cut(s) 128
BstAUI TGTACA 1 cut(s) 471
BstDEI CTNAG 4 cut(s) 36, 211, 384, 389
BstF5I GGATG 2 cut(s) 570, 862
BstHHI GCGC 1 cut(s) 682
BstKTI GATC 2 cut(s) 690, 789
BstMBI GATC 2 cut(s) 687, 786
BstMCI CGRYCG 1 cut(s) 624
BstMWI GCNNNNNNNGC 3 cut(s) 10, 421, 489
BstNSI RCATGY 1 cut(s) 590
BstSFI CTRYAG 2 cut(s) 59, 867
BstV2I GAAGAC 1 cut(s) 210
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 2 cut(s) 222, 931
BtsCI GGATG 2 cut(s) 570, 862
BtsIMutI CAGTG 2 cut(s) 27, 263
CfoI GCGC 1 cut(s) 682
Csp6I GTAC 4 cut(s) 64, 199, 472, 715
CviAII CATG 4 cut(s) 587, 677, 736, 927
CviQI GTAC 4 cut(s) 64, 199, 472, 715
DdeI CTNAG 4 cut(s) 36, 211, 384, 389
DpnI GATC 2 cut(s) 689, 788
DpnII GATC 2 cut(s) 687, 786
DraI TTTAAA 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco24I GRGCYC 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 554
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 4 cut(s) 590, 680, 739, 930
FatI CATG 4 cut(s) 586, 676, 735, 926
FauNDI CATATG 1 cut(s) 225
FbaI TGATCA 1 cut(s) 687
FokI GGATG 2 cut(s) 577, 849
FriOI GRGCYC 1 cut(s) 137
FspBI CTAG 6 cut(s) 98, 174, 351, 431, 462, 683
GlaI GCGC 1 cut(s) 681
HaeIII GGCC 2 cut(s) 222, 931
HapII CCGG 1 cut(s) 19
HhaI GCGC 1 cut(s) 682
Hin1II CATG 4 cut(s) 590, 680, 739, 930
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HinfI GANTC 2 cut(s) 158, 702
HpaII CCGG 1 cut(s) 19
HphI GGTGA 2 cut(s) 153, 580
Hpy188I TCNGA 2 cut(s) 707, 721
Hpy188III TCNNGA 2 cut(s) 944, 996
HpyCH4III ACNGT 6 cut(s) 31, 49, 63, 198, 622, 770
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 4 cut(s) 347, 440, 618, 936
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 421, 489
HpyF3I CTNAG 4 cut(s) 36, 211, 384, 389
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 4 cut(s) 590, 680, 739, 930
HspAI GCGC 1 cut(s) 680
Ksp22I TGATCA 1 cut(s) 687
Kzo9I GATC 2 cut(s) 687, 786
LmnI GCTCC 3 cut(s) 132, 356, 511
LpnPI CCDG 6 cut(s) 32, 198, 287, 297, 345, 546
LweI GCATC 2 cut(s) 160, 356
MaeI CTAG 6 cut(s) 98, 174, 351, 431, 462, 683
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 4 cut(s) 31, 43, 824, 922
MalI GATC 2 cut(s) 689, 788
MboI GATC 2 cut(s) 687, 786
MboII GAAGA 5 cut(s) 115, 215, 269, 563, 841
MfeI CAATTG 2 cut(s) 102, 416
MhlI GDGCHC 2 cut(s) 137, 469
MlsI TGGCCA 1 cut(s) 222
MluCI AATT 9 cut(s) 92, 102, 280, 416, 533, 554, 660, 887, 913
MluNI TGGCCA 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 744
MnlI CCTC 3 cut(s) 414, 519, 683
Mox20I TGGCCA 1 cut(s) 222
MroXI GAANNNNTTC 1 cut(s) 701
MscI TGGCCA 1 cut(s) 222
MseI TTAA 3 cut(s) 317, 519, 581
MslI CAYNNNNRTG 1 cut(s) 563
Msp20I TGGCCA 1 cut(s) 222
MspI CCGG 1 cut(s) 19
MunI CAATTG 2 cut(s) 102, 416
MwoI GCNNNNNNNGC 3 cut(s) 10, 421, 489
NdeI CATATG 1 cut(s) 225
NdeII GATC 2 cut(s) 687, 786
NlaIII CATG 4 cut(s) 590, 680, 739, 930
NlaIV GGNNCC 1 cut(s) 134
NmuCI GTSAC 2 cut(s) 824, 922
NspI RCATGY 1 cut(s) 590
PciI ACATGT 1 cut(s) 586
PcsI WCGNNNNNNNCGW 1 cut(s) 284
PdmI GAANNNNTTC 1 cut(s) 701
PfeI GAWTC 2 cut(s) 158, 702
PscI ACATGT 1 cut(s) 586
PspN4I GGNNCC 1 cut(s) 134
RsaI GTAC 4 cut(s) 65, 200, 473, 716
RsaNI GTAC 4 cut(s) 64, 199, 472, 715
RseI CAYNNNNRTG 1 cut(s) 563
SaqAI TTAA 3 cut(s) 317, 519, 581
Sau3AI GATC 2 cut(s) 687, 786
ScaI AGTACT 1 cut(s) 65
SduI GDGCHC 2 cut(s) 137, 469
SetI ASST 9 cut(s) 60, 217, 238, 316, 330, 361, 390, 696, 900
SfaNI GCATC 2 cut(s) 160, 356
SfcI CTRYAG 2 cut(s) 59, 867
SmiMI CAYNNNNRTG 1 cut(s) 563
Sse9I AATT 9 cut(s) 92, 102, 280, 416, 533, 554, 660, 887, 913
SsiI CCGC 1 cut(s) 514
SspMI CTAG 6 cut(s) 98, 174, 351, 431, 462, 683
TaaI ACNGT 6 cut(s) 31, 49, 63, 198, 622, 770
TaiI ACGT 1 cut(s) 330
TaqI TCGA 3 cut(s) 624, 852, 983
TasI AATT 9 cut(s) 92, 102, 280, 416, 533, 554, 660, 887, 913
TatI WGTACW 2 cut(s) 63, 471
TfiI GAWTC 2 cut(s) 158, 702
Tru1I TTAA 3 cut(s) 317, 519, 581
Tru9I TTAA 3 cut(s) 317, 519, 581
TscAI CASTG 2 cut(s) 34, 270
TseFI GTSAC 2 cut(s) 824, 922
Tsp45I GTSAC 2 cut(s) 824, 922
TspDTI ATGAA 3 cut(s) 41, 528, 827
TspRI CASTG 2 cut(s) 34, 270
XapI RAATTY 5 cut(s) 92, 280, 533, 554, 913
XceI RCATGY 1 cut(s) 590
XmnI GAANNNNTTC 1 cut(s) 701
XspI CTAG 6 cut(s) 98, 174, 351, 431, 462, 683
ZrmI AGTACT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.