MD00G1137100.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000193
Physical Location & Seq
Forward (+)
29737079 .. 29738841
1763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1137100.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGGCAACATTAAGGCTTCAAATATGTATTCTGGCTTTCTGTGCAAGCCTTCTTATTCCAAGCAGCTACAGCCATGGCGAGAAAAAGCATGCAGCCTTGTTCGTCTTTGGTGATTCAGTATTTGATGTCGGAAATAATAACTACGTCAACACTTCCAGTAGCTTTCAGTCAAATAATTGGCCATATGGGGAAACCTTCTTCAATTATGCCACCGGTAGAGCTTCTGATGGTCGTCTAATTCCGGATTTCATCGCTGAGTATGCAAAATTGCCATTTATAATGCCGTATTTACAGCCAGGGTTCAACAATTATGTTTATGGGGTGAACTTTGCATCTGGTGGGGCTGGTGCTCTTGCAGAAACTCATCAAGGATTTGTGATAGACCTTAAAACTCAACTAAGTTATTTCAAGAACGTCGAGAAGCAGTTGAGGCACAAACTAGGCGATGCAGAAGCTTACACATTTCTATCAAATGCTGTTTTCTTGATTAGCATTGGAAGCAATGATTACTTCACCCCATTTGGAACAAATTCAACTTTGTTTGAATCCCACTCACATGAAGAATATGTTGGCATGGTGATTGGGAATCTCACGAATGTGATCAAAGAAATATACAAGAAAGGAGGAAGGAAATTTGGTTTTGCAAACGGCTTTCCTCTCGGTTGTGTACCTAGCATGAGAATACTTAAACCAGAAAGCTTAGGTGCCTGCGTGGAAGAAGTTACAGCATTAGTGAAACTACACAGTAGATTACTTGCTAAAGACCTTCTTAAGCTAAAGACCAAGCTCCAAGGATTCAGATATTCAAACGCAAATTTCTATAATCAAGTAAATGAGATTATTGACAATCCATTAAAATACGGCTTCAAGGAAGGAAAGGCGGCGTGCTGTGGCTCTGGTCCATACAGAGGAATTTCGAGCTGTGGTGGGAAGACAGGCTCCGCAGAGTTTCATTTATGTGAAAATGTTACTGAATATGTTTTCTTTGACTCTGGACACCCAACAGAAAGGGTTTATCAGATACTGTCCAAGCTATGGTGGAGTGGAACTCCGTATGTGACTGGGACTAGCATTCATTTGATGGAGCTATTTGATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

367

Amino Acids

40.61

Weight (kDa)

7.59

Isoelectric Point (pI)

26.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 345 5.4e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 704
AccB7I CCANNNNNTGG 1 cut(s) 1035
AccIII TCCGGA 1 cut(s) 241
AciI CCGC 2 cut(s) 881, 942
AcoI YGGCCR 1 cut(s) 179
AcsI RAATTY 4 cut(s) 529, 632, 814, 912
AfaI GTAC 1 cut(s) 669
AfiI CCNNNNNNNGG 2 cut(s) 908, 1035
AflII CTTAAG 1 cut(s) 770
AgeI ACCGGT 1 cut(s) 212
AgsI TTSAA 8 cut(s) 20, 202, 304, 409, 534, 545, 807, 868
AjnI CCWGG 1 cut(s) 295
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AleI CACNNNNGTG 1 cut(s) 596
Alw21I GWGCWC 1 cut(s) 352
AlwNI CAGNNNCTG 1 cut(s) 1024
Aor13HI TCCGGA 1 cut(s) 241
AoxI GGCC 1 cut(s) 179
ApeKI GCWGC 2 cut(s) 63, 92
ApoI RAATTY 4 cut(s) 529, 632, 814, 912
AsiGI ACCGGT 1 cut(s) 212
Asp700I GAANNNNTTC 1 cut(s) 529
AspS9I GGNCC 1 cut(s) 899
AsuHPI GGTGA 4 cut(s) 122, 334, 505, 589
AvaII GGWCC 1 cut(s) 899
BalI TGGCCA 1 cut(s) 181
BanI GGYRCC 1 cut(s) 704
BbsI GAAGAC 1 cut(s) 938
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 2 cut(s) 75, 104
BccI CCATC 2 cut(s) 221, 1075
BceAI ACGGC 3 cut(s) 268, 664, 877
BciT130I CCWGG 1 cut(s) 297
BclI TGATCA 1 cut(s) 600
BfaI CTAG 3 cut(s) 440, 672, 1068
BfmI CTRYAG 1 cut(s) 67
BfrI CTTAAG 1 cut(s) 770
BisI GCNGC 3 cut(s) 64, 93, 882
BlsI GCNGC 3 cut(s) 65, 94, 883
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 899
BmgT120I GGNCC 1 cut(s) 899
BmiI GGNNCC 2 cut(s) 706, 940
BmrFI CCNGG 1 cut(s) 297
BmrI ACTGGG 1 cut(s) 1071
BmsI GCATC 2 cut(s) 341, 436
BmuI ACTGGG 1 cut(s) 1071
BpiI GAAGAC 1 cut(s) 938
BplI GAGNNNNNCTC 2 cut(s) 1033, 1065
Bpu10I CCTNAGC 1 cut(s) 700
BsaJI CCNNGG 3 cut(s) 73, 296, 790
BsaWI WCCGGW 2 cut(s) 212, 241
Bsc4I CCNNNNNNNGG 2 cut(s) 908, 1035
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 156, 1066
Bse3DI GCAATG 1 cut(s) 508
BseAI TCCGGA 1 cut(s) 241
BseBI CCWGG 1 cut(s) 297
BseDI CCNNGG 3 cut(s) 73, 296, 790
BseLI CCNNNNNNNGG 2 cut(s) 908, 1035
BseMI GCAATG 1 cut(s) 508
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 2 cut(s) 156, 1066
BseXI GCAGC 2 cut(s) 75, 104
BshFI GGCC 1 cut(s) 181
BshNI GGYRCC 1 cut(s) 704
BshTI ACCGGT 1 cut(s) 212
BsiHKAI GWGCWC 1 cut(s) 352
BsiSI CCGG 2 cut(s) 213, 242
BslFI GGGAC 1 cut(s) 1078
BslI CCNNNNNNNGG 2 cut(s) 908, 1035
BsmFI GGGAC 1 cut(s) 1078
BsmI GAATGC 1 cut(s) 1071
BsnI GGCC 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 352
Bsp13I TCCGGA 1 cut(s) 241
Bsp143I GATC 1 cut(s) 600
Bsp19I CCATGG 1 cut(s) 73
BspACI CCGC 2 cut(s) 881, 942
BspANI GGCC 1 cut(s) 181
BspCNI CTCAG 1 cut(s) 247
BspEI TCCGGA 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 706, 940
BspT107I GGYRCC 1 cut(s) 704
BspTI CTTAAG 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 508
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 2 cut(s) 156, 1066
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 3 cut(s) 73, 296, 790
BssMI GATC 1 cut(s) 600
BssT1I CCWWGG 2 cut(s) 73, 790
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 2 cut(s) 746, 1026
BstAFI CTTAAG 1 cut(s) 770
BstC8I GCNNGC 4 cut(s) 46, 90, 709, 886
BstDEI CTNAG 3 cut(s) 255, 398, 700
BstDSI CCRYGG 1 cut(s) 73
BstKTI GATC 1 cut(s) 603
BstMBI GATC 1 cut(s) 600
BstMWI GCNNNNNNNGC 5 cut(s) 41, 69, 260, 430, 498
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 67
BstV1I GCAGC 2 cut(s) 75, 104
BstV2I GAAGAC 1 cut(s) 938
BsuRI GGCC 1 cut(s) 181
BtgI CCRYGG 1 cut(s) 73
BtgZI GCGATG 2 cut(s) 235, 459
Cac8I GCNNGC 4 cut(s) 46, 90, 709, 886
CaiI CAGNNNCTG 1 cut(s) 1024
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 899
Csp6I GTAC 1 cut(s) 668
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 5 cut(s) 74, 89, 557, 574, 676
CviQI GTAC 1 cut(s) 668
DdeI CTNAG 3 cut(s) 255, 398, 700
DpnI GATC 1 cut(s) 602
DpnII GATC 1 cut(s) 600
EaeI YGGCCR 1 cut(s) 179
Eco130I CCWWGG 2 cut(s) 73, 790
Eco47I GGWCC 1 cut(s) 899
EcoRII CCWGG 1 cut(s) 295
EcoT14I CCWWGG 2 cut(s) 73, 790
ErhI CCWWGG 2 cut(s) 73, 790
FaeI CATG 5 cut(s) 77, 92, 560, 577, 679
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FaqI GGGAC 1 cut(s) 1078
FatI CATG 5 cut(s) 73, 88, 556, 573, 675
FauNDI CATATG 1 cut(s) 184
FbaI TGATCA 1 cut(s) 600
Fnu4HI GCNGC 3 cut(s) 64, 93, 882
Fsp4HI GCNGC 3 cut(s) 64, 93, 882
FspBI CTAG 3 cut(s) 440, 672, 1068
GluI GCNGC 3 cut(s) 64, 93, 882
HaeIII GGCC 1 cut(s) 181
HapII CCGG 2 cut(s) 213, 242
Hin1II CATG 5 cut(s) 77, 92, 560, 577, 679
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 2 cut(s) 453, 697
HinfI GANTC 5 cut(s) 113, 545, 586, 795, 989
HpaII CCGG 2 cut(s) 213, 242
HphI GGTGA 4 cut(s) 122, 334, 505, 589
Hpy166II GTNNAC 3 cut(s) 148, 325, 668
Hpy188I TCNGA 4 cut(s) 131, 226, 800, 1020
Hpy188III TCNNGA 6 cut(s) 242, 409, 418, 484, 592, 993
Hpy8I GTNNAC 3 cut(s) 148, 325, 668
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 5 cut(s) 59, 205, 621, 776, 866
HpyCH4III ACNGT 2 cut(s) 746, 1026
HpyCH4IV ACGT 2 cut(s) 144, 414
HpyCH4V TGCA 7 cut(s) 44, 92, 263, 332, 356, 449, 644
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 69, 260, 430, 498
HpyF3I CTNAG 3 cut(s) 255, 398, 700
HpySE526I ACGT 2 cut(s) 144, 414
Hsp92II CATG 5 cut(s) 77, 92, 560, 577, 679
Kpn2I TCCGGA 1 cut(s) 241
Ksp22I TGATCA 1 cut(s) 600
Kzo9I GATC 1 cut(s) 600
LmnI GCTCC 3 cut(s) 792, 944, 1084
Lsp1109I GCAGC 2 cut(s) 75, 104
LweI GCATC 2 cut(s) 341, 436
MaeI CTAG 3 cut(s) 440, 672, 1068
MaeII ACGT 2 cut(s) 144, 414
MaeIII GTNAC 3 cut(s) 721, 967, 1057
MalI GATC 1 cut(s) 602
MboI GATC 1 cut(s) 600
MboII GAAGA 4 cut(s) 190, 572, 728, 943
MhlI GDGCHC 1 cut(s) 352
MlsI TGGCCA 1 cut(s) 181
MluCI AATT 9 cut(s) 175, 202, 237, 266, 307, 529, 632, 814, 912
MluNI TGGCCA 1 cut(s) 181
MlyI GAGTC 1 cut(s) 983
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 4 cut(s) 423, 617, 666, 902
Mox20I TGGCCA 1 cut(s) 181
MroI TCCGGA 1 cut(s) 241
MroXI GAANNNNTTC 1 cut(s) 529
MscI TGGCCA 1 cut(s) 181
MseI TTAA 5 cut(s) 11, 387, 687, 771, 854
MslI CAYNNNNRTG 3 cut(s) 555, 596, 957
Msp20I TGGCCA 1 cut(s) 181
MspCI CTTAAG 1 cut(s) 770
MspI CCGG 2 cut(s) 213, 242
MspR9I CCNGG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 1071
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 5 cut(s) 41, 69, 260, 430, 498
NcoI CCATGG 1 cut(s) 73
NdeI CATATG 1 cut(s) 184
NdeII GATC 1 cut(s) 600
NlaIII CATG 5 cut(s) 77, 92, 560, 577, 679
NlaIV GGNNCC 2 cut(s) 706, 940
NmuCI GTSAC 1 cut(s) 1057
NspI RCATGY 1 cut(s) 92
OliI CACNNNNGTG 1 cut(s) 596
PaeI GCATGC 1 cut(s) 92
PctI GAATGC 1 cut(s) 1071
PdmI GAANNNNTTC 1 cut(s) 529
PfeI GAWTC 4 cut(s) 113, 545, 586, 795
PflMI CCANNNNNTGG 1 cut(s) 1035
PinAI ACCGGT 1 cut(s) 212
PkrI GCNGC 3 cut(s) 65, 94, 883
PleI GAGTC 1 cut(s) 983
PpsI GAGTC 1 cut(s) 983
PsiI TTATAA 1 cut(s) 278
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 2 cut(s) 706, 940
PspPI GGNCC 1 cut(s) 899
PstNI CAGNNNCTG 1 cut(s) 1024
RsaI GTAC 1 cut(s) 669
RsaNI GTAC 1 cut(s) 668
RseI CAYNNNNRTG 3 cut(s) 555, 596, 957
SaqAI TTAA 5 cut(s) 11, 387, 687, 771, 854
SatI GCNGC 3 cut(s) 64, 93, 882
Sau3AI GATC 1 cut(s) 600
Sau96I GGNCC 1 cut(s) 899
SchI GAGTC 1 cut(s) 983
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 1 cut(s) 352
SfaNI GCATC 2 cut(s) 341, 436
SfcI CTRYAG 1 cut(s) 67
SinI GGWCC 1 cut(s) 899
SmiMI CAYNNNNRTG 3 cut(s) 555, 596, 957
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SphI GCATGC 1 cut(s) 92
Sse9I AATT 9 cut(s) 175, 202, 237, 266, 307, 529, 632, 814, 912
SsiI CCGC 2 cut(s) 881, 942
SspMI CTAG 3 cut(s) 440, 672, 1068
StyD4I CCNGG 1 cut(s) 295
StyI CCWWGG 2 cut(s) 73, 790
TaaI ACNGT 2 cut(s) 746, 1026
TaiI ACGT 2 cut(s) 147, 417
TaqI TCGA 2 cut(s) 417, 917
TasI AATT 9 cut(s) 175, 202, 237, 266, 307, 529, 632, 814, 912
TauI GCSGC 1 cut(s) 884
TfiI GAWTC 4 cut(s) 113, 545, 586, 795
Tru1I TTAA 5 cut(s) 11, 387, 687, 771, 854
Tru9I TTAA 5 cut(s) 11, 387, 687, 771, 854
TseFI GTSAC 1 cut(s) 1057
TseI GCWGC 2 cut(s) 63, 92
Tsp45I GTSAC 1 cut(s) 1057
TspDTI ATGAA 4 cut(s) 238, 573, 941, 1064
TspGWI ACGGA 1 cut(s) 1041
Van91I CCANNNNNTGG 1 cut(s) 1035
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 1 cut(s) 899
XapI RAATTY 4 cut(s) 529, 632, 814, 912
XceI RCATGY 1 cut(s) 92
XmnI GAANNNNTTC 1 cut(s) 529
XspI CTAG 3 cut(s) 440, 672, 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.