Rroxscaffold_4G00332370

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000193
Physical Location & Seq
Forward (+)
66511338 .. 66513057
1720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00332370.1

Sequence Viewer

Length: 675 bp
ATGGACACATTTATAGGTGAAGCAGAAGCTCACATATTGCTGTCCGAAGCTGTTTACTTGATTGCCATCGGAGGCAGTGATTACTCTTTCCCATTCATAACGAATTCAAGTTTGTTCGAGTCTCTCTCACATGAAGAATATGTTGGCATGGTGATAGGAAACCTTACAAATGTGATCAAAGAAATATACAAGAAAGGAGGAAGAAAATTTGGGATTAAAGGCATAGGGAATTTGGGTTGTATACCGGGCTTCAGAACAGTTAAACCAGGAAACACGAGCACCTGTAATGAAGAAGTAAATGCACTTTCAAAACTCCACAATAGGGTACTTGCTAAAGCCCTCCTGGAGCTGAAAGGACAGCTCCAAGACTTCATATACTCGAATCCAAATTTCTGCTCTTACGCAAATGACGTAGTTCATAATCCATCAAAACACGGTTTCAAGGAAGCAGAGATGGCATGCTGTGGCTCTGGTCCATACAGAAGAATTAAAAGCTGCGGAGGGAAGCGAGGTGTGACTGAGTATCAGTTCTGTGACAATGTTACTGACTATGTCTACTTTGATTCCAGCCATCCCACAGAACGGTATAACCAGAAACTTTCCAAGTTATGGTGGAGCCATACTTCTGATGTCACAGCATGTTACATCAATTTGAAAGAGCTATTTGAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

224

Amino Acids

25.03

Weight (kDa)

6.65

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 16 - 203 1.4e-10 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 609
AccI GTMKAC 2 cut(s) 241, 555
AciI CCGC 1 cut(s) 498
AcsI RAATTY 4 cut(s) 103, 206, 229, 388
AcuI CTGAAG 1 cut(s) 235
AfaI GTAC 1 cut(s) 327
AfiI CCNNNNNNNGG 3 cut(s) 322, 582, 609
AgsI TTSAA 5 cut(s) 108, 309, 442, 655, 668
AjnI CCWGG 2 cut(s) 265, 342
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 6 cut(s) 29, 50, 349, 361, 495, 661
AluI AGCT 6 cut(s) 29, 50, 349, 361, 495, 661
Alw21I GWGCWC 1 cut(s) 281
Alw26I GTCTC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 495
ApoI RAATTY 4 cut(s) 103, 206, 229, 388
AspS9I GGNCC 1 cut(s) 473
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 2 cut(s) 29, 163
AvaII GGWCC 1 cut(s) 473
BauI CACGAG 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 281
BbvI GCAGC 1 cut(s) 482
BccI CCATC 4 cut(s) 74, 433, 448, 579
BciT130I CCWGG 2 cut(s) 267, 344
BclI TGATCA 1 cut(s) 174
BcnI CCSGG 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 126
BisI GCNGC 1 cut(s) 496
BlsI GCNGC 1 cut(s) 497
Bme1390I CCNGG 3 cut(s) 246, 267, 344
Bme18I GGWCC 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 1 cut(s) 617
BmrFI CCNGG 3 cut(s) 246, 267, 344
BplI GAGNNNNNCTC 2 cut(s) 110, 142
BpmI CTGGAG 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 65
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 582, 609
Bse8I GATNNNNATC 1 cut(s) 65
BseBI CCWGG 2 cut(s) 267, 344
BseGI GGATG 1 cut(s) 571
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 3 cut(s) 322, 582, 609
BseMII CTCAG 1 cut(s) 510
BseXI GCAGC 1 cut(s) 482
BsiHKAI GWGCWC 1 cut(s) 281
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 3 cut(s) 322, 582, 609
BsmAI GTCTC 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 281
Bsp143I GATC 1 cut(s) 174
BspACI CCGC 1 cut(s) 498
BspCNI CTCAG 1 cut(s) 511
BspLI GGNNCC 1 cut(s) 617
BssMI GATC 1 cut(s) 174
BssNAI GTATAC 1 cut(s) 242
BssSI CACGAG 1 cut(s) 274
Bst1107I GTATAC 1 cut(s) 242
Bst2BI CACGAG 1 cut(s) 274
Bst2UI CCWGG 2 cut(s) 267, 344
Bst4CI ACNGT 3 cut(s) 259, 437, 585
BstC8I GCNNGC 1 cut(s) 460
BstDEI CTNAG 1 cut(s) 519
BstF5I GGATG 1 cut(s) 571
BstKTI GATC 1 cut(s) 177
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 1 cut(s) 455
BstNI CCWGG 2 cut(s) 267, 344
BstNSI RCATGY 2 cut(s) 462, 642
BstSCI CCNGG 3 cut(s) 244, 265, 342
BstV1I GCAGC 1 cut(s) 482
BstZ17I GTATAC 1 cut(s) 242
BtsCI GGATG 1 cut(s) 571
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 82
Cac8I GCNNGC 1 cut(s) 460
Cfr13I GGNCC 1 cut(s) 473
Csp6I GTAC 1 cut(s) 326
CviAII CATG 4 cut(s) 131, 148, 459, 639
CviQI GTAC 1 cut(s) 326
DdeI CTNAG 1 cut(s) 519
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 473
Eco57I CTGAAG 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 103
EcoRII CCWGG 2 cut(s) 265, 342
FaeI CATG 4 cut(s) 134, 151, 462, 642
FatI CATG 4 cut(s) 130, 147, 458, 638
FbaI TGATCA 1 cut(s) 174
FblI GTMKAC 2 cut(s) 241, 555
Fnu4HI GCNGC 1 cut(s) 496
FokI GGATG 1 cut(s) 558
Fsp4HI GCNGC 1 cut(s) 496
GluI GCNGC 1 cut(s) 496
GsuI CTGGAG 1 cut(s) 365
HapII CCGG 1 cut(s) 245
Hin1II CATG 4 cut(s) 134, 151, 462, 642
HinfI GANTC 3 cut(s) 119, 382, 563
HpaII CCGG 1 cut(s) 245
HphI GGTGA 2 cut(s) 29, 163
Hpy166II GTNNAC 3 cut(s) 55, 242, 556
Hpy188I TCNGA 4 cut(s) 46, 71, 254, 628
Hpy8I GTNNAC 3 cut(s) 55, 242, 556
HpyCH4III ACNGT 3 cut(s) 259, 437, 585
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 455
HpyF3I CTNAG 1 cut(s) 519
HpySE526I ACGT 1 cut(s) 411
Hsp92II CATG 4 cut(s) 134, 151, 462, 642
Ksp22I TGATCA 1 cut(s) 174
Kzo9I GATC 1 cut(s) 174
LmnI GCTCC 3 cut(s) 346, 366, 615
LpnPI CCDG 9 cut(s) 252, 258, 279, 295, 329, 356, 456, 580, 605
Lsp1109I GCAGC 1 cut(s) 482
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 5 cut(s) 514, 533, 541, 631, 641
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MboII GAAGA 4 cut(s) 146, 213, 302, 495
MhlI GDGCHC 1 cut(s) 281
MluCI AATT 6 cut(s) 103, 206, 229, 388, 486, 649
MlyI GAGTC 1 cut(s) 128
MnlI CCTC 5 cut(s) 65, 191, 350, 494, 503
MseI TTAA 3 cut(s) 216, 261, 489
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 3 cut(s) 246, 267, 344
MvaI CCWGG 2 cut(s) 267, 344
MwoI GCNNNNNNNGC 1 cut(s) 455
NciI CCSGG 1 cut(s) 246
NdeII GATC 1 cut(s) 174
NlaIII CATG 4 cut(s) 134, 151, 462, 642
NlaIV GGNNCC 1 cut(s) 617
NmuCI GTSAC 3 cut(s) 514, 533, 631
NspI RCATGY 2 cut(s) 462, 642
PaeI GCATGC 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PfeI GAWTC 2 cut(s) 382, 563
PflFI GACNNNGTC 1 cut(s) 551
PflMI CCANNNNNTGG 1 cut(s) 609
PfoI TCCNGGA 1 cut(s) 342
PkrI GCNGC 1 cut(s) 497
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 265, 342
PspGI CCWGG 2 cut(s) 265, 342
PspN4I GGNNCC 1 cut(s) 617
PspPI GGNCC 1 cut(s) 473
PsyI GACNNNGTC 1 cut(s) 551
RsaI GTAC 1 cut(s) 327
RsaNI GTAC 1 cut(s) 326
SaqAI TTAA 3 cut(s) 216, 261, 489
SatI GCNGC 1 cut(s) 496
Sau3AI GATC 1 cut(s) 174
Sau96I GGNCC 1 cut(s) 473
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 3 cut(s) 246, 267, 344
SduI GDGCHC 1 cut(s) 281
SinI GGWCC 1 cut(s) 473
SphI GCATGC 1 cut(s) 462
Sse9I AATT 6 cut(s) 103, 206, 229, 388, 486, 649
SsiI CCGC 1 cut(s) 498
StyD4I CCNGG 3 cut(s) 244, 265, 342
TaaI ACNGT 3 cut(s) 259, 437, 585
TaiI ACGT 1 cut(s) 414
TaqI TCGA 2 cut(s) 117, 380
TasI AATT 6 cut(s) 103, 206, 229, 388, 486, 649
TfiI GAWTC 2 cut(s) 382, 563
Tru1I TTAA 3 cut(s) 216, 261, 489
Tru9I TTAA 3 cut(s) 216, 261, 489
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 3 cut(s) 514, 533, 631
TseI GCWGC 1 cut(s) 495
Tsp45I GTSAC 3 cut(s) 514, 533, 631
TspDTI ATGAA 5 cut(s) 85, 147, 303, 361, 407
TspRI CASTG 1 cut(s) 82
Tth111I GACNNNGTC 1 cut(s) 551
Van91I CCANNNNNTGG 1 cut(s) 609
VpaK11BI GGWCC 1 cut(s) 473
XapI RAATTY 4 cut(s) 103, 206, 229, 388
XceI RCATGY 2 cut(s) 462, 642
XmiI GTMKAC 2 cut(s) 241, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.