FvH4_6g35580

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000193
Physical Location & Seq
Reverse (-)
28082961 .. 28085114
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35580.t1

Sequence Viewer

Length: 1116 bp
ATGGCAACTCGTTCAAGATTACAAATATATGTCCTGGCTTTTTGTGCAACCCTTCTTGTTCAAAGTGGCTGCAATGGCCTTTCTGTGCATCACAGAAAACATGCAGCCTTGTTCATCTTTGGGGATTCACTATTTGATGTTGGAAATAATAACTACATAAACACTTCCACCAACTTTCAAGCAAATTTCTTCCCATATGGGGAAACCTTCTTCGGGTACTCGACTGGTAGGGTTTCCGATGGTCGTCTAATCCCAGATATCATAGCTGAATATGCAAACTTGCCAATGATTCCACCATACTTACAACCCGGTTTCGACAACTATACTAATGGAGTGAACTTTGCATCTGCTGGGGCTGGTGCTCTAGCTGAAACGTTTCAAGGATTTGTGTTAGACCTTAAAACTCAGCTGGGTTATTTCAAGAATGTGGAGAAGCAGTTGAGGCACAGAGTAGGTGAAGCAGAAGCTCACACATTGTTGTCCGAAGCTGTTTACTTGATTAGCATCGGAAGCAATGATTATATTGCCCCATTCATAGCGAATTCAAGTTTATTCGAGTCCCGCTCACATGAAGAATATGTTCGCATGGTGACAGGAAACCTGACAAATGTGATCAAAGAAATATACAGGAAAGGAGGGAGGAAATTTGGGATTGCAGGCATGGAGCCTTTGGGTTGTTTACCAGGAATGAGAACAATCAAACCAGGGAACACAACCACCTGTGGAGAAGAAATCAATGATATTTCAAGACTCCACAACAGAGTTCTTGCTAAAGTCCTCCTGAAGCTCAAAGGACAACTCCCAGAATTCATATACTCAAATCCAAACTTCTACTCATACCTAAATGACACAGTTCATAATCCATCAAAACATGGTTTCAAGGAAGGAAAGATGGCATGCTGTGGCTCTGGTCCATACAGAGGAATTATGAGCTGCGGAGGGAAGCGAGGTGTGACTGAGTATCAGTTATGTGAAAATGTTACTGACTATGTCTTTTTTGATTCCGGCCATGCAACGGAAAGAATTTACCAGCAAGTTTCCAAGTTATGGTGGAGCCATAGTACTCCTGATGTCACAGCACGTTACATCAATTTGAAAGAGCTATTTGAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

372

Amino Acids

41.42

Weight (kDa)

8.04

Isoelectric Point (pI)

25.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 349 4.2e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1047
AccBSI CCGCTC 1 cut(s) 564
AciI CCGC 2 cut(s) 562, 936
AclI AACGTT 1 cut(s) 374
AcoI YGGCCR 1 cut(s) 1006
AcsI RAATTY 5 cut(s) 184, 541, 644, 806, 1023
AcuI CTGAAG 1 cut(s) 803
AfaI GTAC 2 cut(s) 218, 1063
AfiI CCNNNNNNNGG 5 cut(s) 199, 213, 920, 1015, 1047
AjnI CCWGG 3 cut(s) 33, 682, 703
AluBI AGCT 8 cut(s) 266, 368, 409, 467, 488, 787, 933, 1102
AluI AGCT 8 cut(s) 266, 368, 409, 467, 488, 787, 933, 1102
Alw21I GWGCWC 1 cut(s) 364
AoxI GGCC 2 cut(s) 76, 1006
ApeKI GCWGC 3 cut(s) 69, 104, 933
ApoI RAATTY 5 cut(s) 184, 541, 644, 806, 1023
Asp700I GAANNNNTTC 2 cut(s) 375, 579
AspS9I GGNCC 1 cut(s) 911
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 2 cut(s) 467, 601
AvaII GGWCC 1 cut(s) 911
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 3 cut(s) 56, 116, 920
BccI CCATC 3 cut(s) 233, 871, 886
BciT130I CCWGG 3 cut(s) 35, 684, 705
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 1 cut(s) 309
BfaI CTAG 1 cut(s) 365
BisI GCNGC 3 cut(s) 70, 105, 934
BlsI GCNGC 3 cut(s) 71, 106, 935
BmcAI AGTACT 1 cut(s) 1063
Bme1390I CCNGG 4 cut(s) 35, 309, 684, 705
Bme18I GGWCC 1 cut(s) 911
BmgT120I GGNCC 1 cut(s) 911
BmiI GGNNCC 2 cut(s) 666, 1055
BmrFI CCNGG 4 cut(s) 35, 309, 684, 705
BmsI GCATC 3 cut(s) 97, 353, 513
BplI GAGNNNNNCTC 2 cut(s) 548, 580
BpuMI CCSGG 1 cut(s) 309
BsaBI GATNNNNATC 1 cut(s) 503
BsaJI CCNNGG 1 cut(s) 704
BsaXI ACNNNNNCTCC 4 cut(s) 422, 452, 1045, 1075
Bsc4I CCNNNNNNNGG 5 cut(s) 199, 213, 920, 1015, 1047
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 2 cut(s) 79, 520
Bse8I GATNNNNATC 1 cut(s) 503
BseBI CCWGG 3 cut(s) 35, 684, 705
BseDI CCNNGG 1 cut(s) 704
BseJI GATNNNNATC 1 cut(s) 503
BseLI CCNNNNNNNGG 5 cut(s) 199, 213, 920, 1015, 1047
BseMI GCAATG 2 cut(s) 79, 520
BseMII CTCAG 2 cut(s) 419, 948
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 3 cut(s) 56, 116, 920
BseYI CCCAGC 2 cut(s) 350, 409
BshFI GGCC 2 cut(s) 78, 1008
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 2 cut(s) 309, 1005
BslFI GGGAC 1 cut(s) 544
BslI CCNNNNNNNGG 5 cut(s) 199, 213, 920, 1015, 1047
BsmFI GGGAC 1 cut(s) 544
BsnI GGCC 2 cut(s) 78, 1008
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 1 cut(s) 612
BspACI CCGC 2 cut(s) 562, 936
BspANI GGCC 2 cut(s) 78, 1008
BspCNI CTCAG 2 cut(s) 418, 949
BspLI GGNNCC 2 cut(s) 666, 1055
BsrBI CCGCTC 1 cut(s) 564
BsrDI GCAATG 2 cut(s) 79, 520
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 704
BssMI GATC 1 cut(s) 612
Bst2UI CCWGG 3 cut(s) 35, 684, 705
Bst4CI ACNGT 1 cut(s) 853
BstC8I GCNNGC 2 cut(s) 658, 898
BstDEI CTNAG 2 cut(s) 405, 957
BstKTI GATC 1 cut(s) 615
BstMBI GATC 1 cut(s) 612
BstMWI GCNNNNNNNGC 5 cut(s) 44, 75, 272, 442, 510
BstNI CCWGG 3 cut(s) 35, 684, 705
BstNSI RCATGY 2 cut(s) 104, 900
BstSCI CCNGG 4 cut(s) 33, 307, 682, 703
BstV1I GCAGC 3 cut(s) 56, 116, 920
BsuRI GGCC 2 cut(s) 78, 1008
Cac8I GCNNGC 2 cut(s) 658, 898
Cfr13I GGNCC 1 cut(s) 911
Csp6I GTAC 2 cut(s) 217, 1062
CviAII CATG 7 cut(s) 101, 569, 586, 661, 872, 897, 1010
CviQI GTAC 2 cut(s) 217, 1062
DdeI CTNAG 2 cut(s) 405, 957
DpnI GATC 1 cut(s) 614
DpnII GATC 1 cut(s) 612
EaeI YGGCCR 1 cut(s) 1006
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 911
Eco57I CTGAAG 1 cut(s) 803
EcoRI GAATTC 2 cut(s) 541, 806
EcoRII CCWGG 3 cut(s) 33, 682, 703
EcoRV GATATC 1 cut(s) 259
FaeI CATG 7 cut(s) 104, 572, 589, 664, 875, 900, 1013
FaqI GGGAC 1 cut(s) 544
FatI CATG 7 cut(s) 100, 568, 585, 660, 871, 896, 1009
FauI CCCGC 1 cut(s) 569
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 612
Fnu4HI GCNGC 3 cut(s) 70, 105, 934
Fsp4HI GCNGC 3 cut(s) 70, 105, 934
FspBI CTAG 1 cut(s) 365
GluI GCNGC 3 cut(s) 70, 105, 934
GsaI CCCAGC 2 cut(s) 354, 413
HaeIII GGCC 2 cut(s) 78, 1008
HapII CCGG 2 cut(s) 309, 1005
Hin1II CATG 7 cut(s) 104, 572, 589, 664, 875, 900, 1013
HinfI GANTC 5 cut(s) 125, 289, 557, 750, 1001
HpaII CCGG 2 cut(s) 309, 1005
HphI GGTGA 2 cut(s) 467, 601
Hpy166II GTNNAC 3 cut(s) 337, 493, 680
Hpy188I TCNGA 3 cut(s) 238, 484, 509
Hpy188III TCNNGA 5 cut(s) 15, 421, 747, 781, 1067
Hpy8I GTNNAC 3 cut(s) 337, 493, 680
HpyAV CCTTC 3 cut(s) 62, 217, 878
HpyCH4III ACNGT 1 cut(s) 853
HpyCH4IV ACGT 2 cut(s) 374, 1081
HpyCH4V TGCA 8 cut(s) 47, 72, 88, 104, 275, 344, 656, 1013
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 75, 272, 442, 510
HpyF3I CTNAG 2 cut(s) 405, 957
HpySE526I ACGT 2 cut(s) 374, 1081
Hsp92II CATG 7 cut(s) 104, 572, 589, 664, 875, 900, 1013
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 1 cut(s) 612
LmnI GCTCC 2 cut(s) 664, 1053
Lsp1109I GCAGC 3 cut(s) 56, 116, 920
LweI GCATC 3 cut(s) 97, 353, 513
MaeI CTAG 1 cut(s) 365
MaeII ACGT 2 cut(s) 374, 1081
MaeIII GTNAC 5 cut(s) 589, 952, 979, 1072, 1082
MalI GATC 1 cut(s) 614
MbiI CCGCTC 1 cut(s) 564
MboI GATC 1 cut(s) 612
MboII GAAGA 4 cut(s) 181, 202, 584, 740
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 7 cut(s) 184, 541, 644, 806, 924, 1023, 1090
MlyI GAGTC 2 cut(s) 566, 744
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 7 cut(s) 435, 629, 633, 788, 914, 932, 941
MroXI GAANNNNTTC 2 cut(s) 375, 579
MseI TTAA 1 cut(s) 399
MspA1I CMGCKG 1 cut(s) 409
MspI CCGG 2 cut(s) 309, 1005
MspR9I CCNGG 4 cut(s) 35, 309, 684, 705
MvaI CCWGG 3 cut(s) 35, 684, 705
MwoI GCNNNNNNNGC 5 cut(s) 44, 75, 272, 442, 510
NciI CCSGG 1 cut(s) 309
NdeI CATATG 1 cut(s) 196
NdeII GATC 1 cut(s) 612
NlaIII CATG 7 cut(s) 104, 572, 589, 664, 875, 900, 1013
NlaIV GGNNCC 2 cut(s) 666, 1055
NmuCI GTSAC 3 cut(s) 589, 952, 1072
NspI RCATGY 2 cut(s) 104, 900
PaeI GCATGC 1 cut(s) 900
PdmI GAANNNNTTC 2 cut(s) 375, 579
PfeI GAWTC 3 cut(s) 125, 289, 1001
PflFI GACNNNGTC 1 cut(s) 989
PflMI CCANNNNNTGG 1 cut(s) 1047
PkrI GCNGC 3 cut(s) 71, 106, 935
PleI GAGTC 2 cut(s) 565, 744
PpsI GAGTC 2 cut(s) 565, 744
Psp1406I AACGTT 1 cut(s) 374
Psp6I CCWGG 3 cut(s) 33, 682, 703
PspFI CCCAGC 2 cut(s) 350, 409
PspGI CCWGG 3 cut(s) 33, 682, 703
PspN4I GGNNCC 2 cut(s) 666, 1055
PspPI GGNCC 1 cut(s) 911
PsyI GACNNNGTC 1 cut(s) 989
PvuII CAGCTG 1 cut(s) 409
RsaI GTAC 2 cut(s) 218, 1063
RsaNI GTAC 2 cut(s) 217, 1062
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 3 cut(s) 70, 105, 934
Sau3AI GATC 1 cut(s) 612
Sau96I GGNCC 1 cut(s) 911
ScaI AGTACT 1 cut(s) 1063
SchI GAGTC 2 cut(s) 566, 744
ScrFI CCNGG 4 cut(s) 35, 309, 684, 705
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 3 cut(s) 97, 353, 513
SinI GGWCC 1 cut(s) 911
SphI GCATGC 1 cut(s) 900
Sse9I AATT 7 cut(s) 184, 541, 644, 806, 924, 1023, 1090
SsiI CCGC 2 cut(s) 562, 936
SspMI CTAG 1 cut(s) 365
StyD4I CCNGG 4 cut(s) 33, 307, 682, 703
TaaI ACNGT 1 cut(s) 853
TaiI ACGT 2 cut(s) 377, 1084
TaqI TCGA 3 cut(s) 221, 315, 555
TasI AATT 7 cut(s) 184, 541, 644, 806, 924, 1023, 1090
TatI WGTACW 1 cut(s) 1061
TfiI GAWTC 3 cut(s) 125, 289, 1001
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TseFI GTSAC 3 cut(s) 589, 952, 1072
TseI GCWGC 3 cut(s) 69, 104, 933
Tsp45I GTSAC 3 cut(s) 589, 952, 1072
TspDTI ATGAA 5 cut(s) 103, 523, 585, 799, 845
TspGWI ACGGA 1 cut(s) 1031
Tth111I GACNNNGTC 1 cut(s) 989
Van91I CCANNNNNTGG 1 cut(s) 1047
VpaK11BI GGWCC 1 cut(s) 911
XapI RAATTY 5 cut(s) 184, 541, 644, 806, 1023
XceI RCATGY 2 cut(s) 104, 900
XmnI GAANNNNTTC 2 cut(s) 375, 579
XspI CTAG 1 cut(s) 365
ZrmI AGTACT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.