Prupe.3G033400_v2.0.a1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000193
Physical Location & Seq
Forward (+)
2464069 .. 2466110
2042 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G033400.1

Sequence Viewer

Length: 1065 bp
ATGGCATCTTTCAGGTTTCAAATCTATATATTGGCTGTCTGTGCAGGCCTTGTTATTCAAAGCAGCTGCAATGGCCATTCTGGGCTTCAGAAAAAACATGTAGCCCTGTTCATCTTTGGTGATTCACTCTATGATCCTGGAAATAATAACTACATAAATACCACAAAAGATTTCCAGGCAAATTGGTTGCCATATGGTGAAACATTCTTCAGGTACCCAACTGGTAGATTTTCTGATGGGCGTTTAATCCCAGATTTCATTGCTGAGTATGCCAAGTTGCCAATTATACCAGCGTATTTACAACCTGGGTTGAAAGACTATACTTATGGGGTGAACTTTGCTTCTGGTGGAGCTGGTGCTCTAGTTGAAAGCCATCAAGGATTTGTGATAGACCTTAAAACTCAACGAAGTCAATTCAAGAAGGTGGAGAAGCAGTTGAGGCAGAAACTAGGTGAAGCAGAAGCCTACACATTGCTGTCCAAAGCTGTCTACTTAATCAGCATTGGAAGCAATGACTATTCTATCCCATTAGCAACCAATACCAGCCATGACGAATATGTTGGCTGGGTTATTGGCAACCTGACAAGTTGGATCAAAGATGTATACAAGAAAGGAGGCAGGAAATTTGGATTTTCAAGCTTGGCCCCTCTTGCTTCTGTGCCAAGCATGAGAGTGATTCAACCAGGAAACACAGGCCCTTCCGGGGAAGAAGTTACAGCACTTGTGAAACTACACAATAGACTACTTTCCAAAGTCCTCACAAAGCTGAAGAAAGAGCTCCAAGGATTCAAATACTCAAAGCTTAATCTCTACACTTACGCTAAAGAAAGAATTAATCATCCATCAAAATATGGTTTCAAGGAAGGAAAGGCGGCGTGCTGTGGCTCTGGCCCATACGGAGGAATTTATACCTGTGGAGGGAAGAGAGGTGTGACTGAGTATGAGTTATGTGGCAATGTTACTGAGTATGTCTTTTTTGACTCTGTCCATCCAACTGAAAGGGTTTACGAGCAAGTGTCCAAGTTATGGTGGGGACAAAACTTGAAGGAGCTCTTTGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

355

Amino Acids

39.52

Weight (kDa)

9.15

Isoelectric Point (pI)

22.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 213
AccB1I GGYRCC 1 cut(s) 213
AccB7I CCANNNNNTGG 1 cut(s) 1026
AccI GTMKAC 2 cut(s) 489, 603
AciI CCGC 1 cut(s) 872
AclWI GGATC 2 cut(s) 128, 599
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 2 cut(s) 623, 903
AcuI CTGAAG 3 cut(s) 71, 193, 788
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 4 cut(s) 703, 899, 918, 1026
AflIII ACRYGT 1 cut(s) 97
AjnI CCWGG 4 cut(s) 136, 174, 304, 682
AluBI AGCT 8 cut(s) 66, 353, 485, 639, 766, 778, 802, 1051
AluI AGCT 8 cut(s) 66, 353, 485, 639, 766, 778, 802, 1051
Alw21I GWGCWC 3 cut(s) 361, 780, 1053
AlwI GGATC 2 cut(s) 128, 599
AoxI GGCC 5 cut(s) 46, 73, 642, 694, 889
ApeKI GCWGC 2 cut(s) 63, 66
ApoI RAATTY 2 cut(s) 623, 903
AseI ATTAAT 1 cut(s) 834
Asp718I GGTACC 1 cut(s) 213
AspS9I GGNCC 3 cut(s) 643, 695, 890
AsuC2I CCSGG 1 cut(s) 703
AsuHPI GGTGA 4 cut(s) 131, 209, 343, 464
BalI TGGCCA 1 cut(s) 75
BanI GGYRCC 1 cut(s) 213
BanII GRGCYC 2 cut(s) 780, 1053
Bbv12I GWGCWC 3 cut(s) 361, 780, 1053
BbvI GCAGC 2 cut(s) 53, 75
BccI CCATC 4 cut(s) 230, 381, 850, 996
BciT130I CCWGG 4 cut(s) 138, 176, 306, 684
BcnI CCSGG 1 cut(s) 703
BfaI CTAG 2 cut(s) 362, 449
BisI GCNGC 3 cut(s) 64, 67, 873
BlsI GCNGC 3 cut(s) 65, 68, 874
Bme1390I CCNGG 5 cut(s) 138, 176, 306, 684, 703
BmgT120I GGNCC 3 cut(s) 643, 695, 890
BmiI GGNNCC 2 cut(s) 215, 645
BmrFI CCNGG 5 cut(s) 138, 176, 306, 684, 703
BmsI GCATC 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 703
BsaJI CCNNGG 3 cut(s) 305, 702, 781
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 4 cut(s) 703, 899, 918, 1026
Bse1I ACTGG 1 cut(s) 226
Bse3DI GCAATG 5 cut(s) 76, 258, 470, 517, 961
BseBI CCWGG 4 cut(s) 138, 176, 306, 684
BseDI CCNNGG 3 cut(s) 305, 702, 781
BseGI GGATG 2 cut(s) 838, 988
BseLI CCNNNNNNNGG 4 cut(s) 703, 899, 918, 1026
BseMI GCAATG 5 cut(s) 76, 258, 470, 517, 961
BseMII CTCAG 3 cut(s) 255, 927, 954
BseNI ACTGG 1 cut(s) 226
BseXI GCAGC 2 cut(s) 53, 75
BseYI CCCAGC 1 cut(s) 564
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 5 cut(s) 48, 75, 644, 696, 891
BshNI GGYRCC 1 cut(s) 213
BsiHKAI GWGCWC 3 cut(s) 361, 780, 1053
BsiSI CCGG 1 cut(s) 702
BslFI GGGAC 1 cut(s) 1047
BslI CCNNNNNNNGG 4 cut(s) 703, 899, 918, 1026
BsmFI GGGAC 1 cut(s) 1047
BsnI GGCC 5 cut(s) 48, 75, 644, 696, 891
Bsp1286I GDGCHC 3 cut(s) 361, 780, 1053
Bsp143I GATC 2 cut(s) 133, 591
BspACI CCGC 1 cut(s) 872
BspANI GGCC 5 cut(s) 48, 75, 644, 696, 891
BspCNI CTCAG 3 cut(s) 256, 928, 955
BspLI GGNNCC 2 cut(s) 215, 645
BspPI GGATC 2 cut(s) 128, 599
BspT107I GGYRCC 1 cut(s) 213
BsrDI GCAATG 5 cut(s) 76, 258, 470, 517, 961
BsrI ACTGG 1 cut(s) 226
BssECI CCNNGG 3 cut(s) 305, 702, 781
BssMI GATC 2 cut(s) 133, 591
BssNAI GTATAC 1 cut(s) 604
BssT1I CCWWGG 1 cut(s) 781
Bst1107I GTATAC 1 cut(s) 604
Bst2UI CCWGG 4 cut(s) 138, 176, 306, 684
Bst6I CTCTTC 1 cut(s) 917
BstC8I GCNNGC 2 cut(s) 46, 877
BstDEI CTNAG 3 cut(s) 264, 936, 963
BstF5I GGATG 2 cut(s) 838, 988
BstKTI GATC 2 cut(s) 136, 594
BstMBI GATC 2 cut(s) 133, 591
BstMWI GCNNNNNNNGC 6 cut(s) 41, 72, 269, 439, 507, 650
BstNI CCWGG 4 cut(s) 138, 176, 306, 684
BstNSI RCATGY 1 cut(s) 101
BstSCI CCNGG 5 cut(s) 136, 174, 304, 682, 701
BstV1I GCAGC 2 cut(s) 53, 75
BstZ17I GTATAC 1 cut(s) 604
BsuRI GGCC 5 cut(s) 48, 75, 644, 696, 891
BtsCI GGATG 2 cut(s) 838, 988
Cac8I GCNNGC 2 cut(s) 46, 877
Cfr13I GGNCC 3 cut(s) 643, 695, 890
Csp6I GTAC 1 cut(s) 214
CviAII CATG 3 cut(s) 98, 548, 667
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 3 cut(s) 264, 936, 963
DpnI GATC 2 cut(s) 135, 593
DpnII GATC 2 cut(s) 133, 591
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 917
EarI CTCTTC 1 cut(s) 917
Ecl136II GAGCTC 2 cut(s) 778, 1051
Eco130I CCWWGG 1 cut(s) 781
Eco147I AGGCCT 1 cut(s) 48
Eco24I GRGCYC 2 cut(s) 780, 1053
Eco53kI GAGCTC 2 cut(s) 778, 1051
Eco57I CTGAAG 3 cut(s) 71, 193, 788
EcoICRI GAGCTC 2 cut(s) 778, 1051
EcoO109I RGGNCCY 1 cut(s) 695
EcoRII CCWGG 4 cut(s) 136, 174, 304, 682
EcoT14I CCWWGG 1 cut(s) 781
EcoT38I GRGCYC 2 cut(s) 780, 1053
ErhI CCWWGG 1 cut(s) 781
FaeI CATG 3 cut(s) 101, 551, 670
FalI AAGNNNNNCTT 1 cut(s) 1037
FaqI GGGAC 1 cut(s) 1047
FatI CATG 3 cut(s) 97, 547, 666
FauNDI CATATG 1 cut(s) 193
FblI GTMKAC 2 cut(s) 489, 603
Fnu4HI GCNGC 3 cut(s) 64, 67, 873
FokI GGATG 2 cut(s) 825, 975
FriOI GRGCYC 2 cut(s) 780, 1053
Fsp4HI GCNGC 3 cut(s) 64, 67, 873
FspBI CTAG 2 cut(s) 362, 449
GluI GCNGC 3 cut(s) 64, 67, 873
GsaI CCCAGC 1 cut(s) 568
HaeIII GGCC 5 cut(s) 48, 75, 644, 696, 891
HapII CCGG 1 cut(s) 702
Hin1II CATG 3 cut(s) 101, 551, 670
HindIII AAGCTT 2 cut(s) 637, 800
HinfI GANTC 4 cut(s) 122, 676, 786, 980
HpaII CCGG 1 cut(s) 702
HphI GGTGA 4 cut(s) 131, 209, 343, 464
Hpy166II GTNNAC 4 cut(s) 334, 490, 604, 1006
Hpy188I TCNGA 2 cut(s) 90, 235
Hpy188III TCNNGA 1 cut(s) 418
Hpy8I GTNNAC 4 cut(s) 334, 490, 604, 1006
HpyAV CCTTC 4 cut(s) 415, 708, 857, 1039
HpyCH4V TGCA 2 cut(s) 44, 69
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 72, 269, 439, 507, 650
HpyF3I CTNAG 3 cut(s) 264, 936, 963
Hsp92II CATG 3 cut(s) 101, 551, 670
KpnI GGTACC 1 cut(s) 217
Kzo9I GATC 2 cut(s) 133, 591
LmnI GCTCC 3 cut(s) 350, 783, 1048
Lsp1109I GCAGC 2 cut(s) 53, 75
LweI GCATC 1 cut(s) 14
MaeI CTAG 2 cut(s) 362, 449
MaeIII GTNAC 3 cut(s) 712, 931, 958
MalI GATC 2 cut(s) 135, 593
MboI GATC 2 cut(s) 133, 591
MboII GAAGA 4 cut(s) 199, 719, 781, 934
MhlI GDGCHC 3 cut(s) 361, 780, 1053
MlsI TGGCCA 1 cut(s) 75
MluCI AATT 6 cut(s) 181, 282, 413, 623, 831, 903
MluNI TGGCCA 1 cut(s) 75
MlyI GAGTC 1 cut(s) 974
MmeI TCCRAC 2 cut(s) 569, 1016
MnlI CCTC 7 cut(s) 432, 608, 657, 767, 893, 911, 920
Mox20I TGGCCA 1 cut(s) 75
MscI TGGCCA 1 cut(s) 75
MseI TTAA 5 cut(s) 245, 396, 494, 804, 834
MslI CAYNNNNRTG 1 cut(s) 671
Msp20I TGGCCA 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 1 cut(s) 702
MspR9I CCNGG 5 cut(s) 138, 176, 306, 684, 703
MvaI CCWGG 4 cut(s) 138, 176, 306, 684
MwoI GCNNNNNNNGC 6 cut(s) 41, 72, 269, 439, 507, 650
NciI CCSGG 1 cut(s) 703
NdeI CATATG 1 cut(s) 193
NdeII GATC 2 cut(s) 133, 591
NlaIII CATG 3 cut(s) 101, 551, 670
NlaIV GGNNCC 2 cut(s) 215, 645
NmuCI GTSAC 1 cut(s) 931
NspI RCATGY 1 cut(s) 101
PceI AGGCCT 1 cut(s) 48
PciI ACATGT 1 cut(s) 97
PfeI GAWTC 3 cut(s) 122, 676, 786
PflFI GACNNNGTC 1 cut(s) 983
PflMI CCANNNNNTGG 1 cut(s) 1026
PfoI TCCNGGA 1 cut(s) 136
PkrI GCNGC 3 cut(s) 65, 68, 874
PleI GAGTC 1 cut(s) 974
PpsI GAGTC 1 cut(s) 974
PscI ACATGT 1 cut(s) 97
PshBI ATTAAT 1 cut(s) 834
Psp124BI GAGCTC 2 cut(s) 780, 1053
Psp6I CCWGG 4 cut(s) 136, 174, 304, 682
PspFI CCCAGC 1 cut(s) 564
PspGI CCWGG 4 cut(s) 136, 174, 304, 682
PspN4I GGNNCC 2 cut(s) 215, 645
PspPI GGNCC 3 cut(s) 643, 695, 890
PsyI GACNNNGTC 1 cut(s) 983
PvuII CAGCTG 1 cut(s) 66
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
RseI CAYNNNNRTG 1 cut(s) 671
SacI GAGCTC 2 cut(s) 780, 1053
SaqAI TTAA 5 cut(s) 245, 396, 494, 804, 834
SatI GCNGC 3 cut(s) 64, 67, 873
Sau3AI GATC 2 cut(s) 133, 591
Sau96I GGNCC 3 cut(s) 643, 695, 890
SchI GAGTC 1 cut(s) 974
ScrFI CCNGG 5 cut(s) 138, 176, 306, 684, 703
SduI GDGCHC 3 cut(s) 361, 780, 1053
SfaNI GCATC 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 671
Sse9I AATT 6 cut(s) 181, 282, 413, 623, 831, 903
SseBI AGGCCT 1 cut(s) 48
SsiI CCGC 1 cut(s) 872
SspMI CTAG 2 cut(s) 362, 449
SstI GAGCTC 2 cut(s) 780, 1053
StuI AGGCCT 1 cut(s) 48
StyD4I CCNGG 5 cut(s) 136, 174, 304, 682, 701
StyI CCWWGG 1 cut(s) 781
TasI AATT 6 cut(s) 181, 282, 413, 623, 831, 903
TauI GCSGC 1 cut(s) 875
TfiI GAWTC 3 cut(s) 122, 676, 786
Tru1I TTAA 5 cut(s) 245, 396, 494, 804, 834
Tru9I TTAA 5 cut(s) 245, 396, 494, 804, 834
TseFI GTSAC 1 cut(s) 931
TseI GCWGC 2 cut(s) 63, 66
Tsp45I GTSAC 1 cut(s) 931
TspDTI ATGAA 2 cut(s) 100, 247
TspGWI ACGGA 1 cut(s) 912
Tth111I GACNNNGTC 1 cut(s) 983
Van91I CCANNNNNTGG 1 cut(s) 1026
VspI ATTAAT 1 cut(s) 834
XapI RAATTY 2 cut(s) 623, 903
XceI RCATGY 1 cut(s) 101
XmiI GTMKAC 2 cut(s) 489, 603
XspI CTAG 2 cut(s) 362, 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.