Belongs to the 'GDSL' lipolytic enzyme family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 181 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 99 |
| AgsI | TTSAA | 3 cut(s) | 76, 124, 132 |
| AluBI | AGCT | 1 cut(s) | 112 |
| AluI | AGCT | 1 cut(s) | 112 |
| ApoI | RAATTY | 1 cut(s) | 181 |
| BfaI | CTAG | 1 cut(s) | 193 |
| BfmI | CTRYAG | 1 cut(s) | 145 |
| BsaJI | CCNNGG | 1 cut(s) | 115 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 99 |
| BseDI | CCNNGG | 1 cut(s) | 115 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 99 |
| BseRI | GAGGAG | 1 cut(s) | 32 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 99 |
| BssECI | CCNNGG | 1 cut(s) | 115 |
| BssT1I | CCWWGG | 1 cut(s) | 115 |
| Bst4CI | ACNGT | 1 cut(s) | 149 |
| BstSFI | CTRYAG | 1 cut(s) | 145 |
| CviJI | RGCY | 1 cut(s) | 112 |
| CviKI_1 | RGCY | 1 cut(s) | 112 |
| Eco130I | CCWWGG | 1 cut(s) | 115 |
| EcoT14I | CCWWGG | 1 cut(s) | 115 |
| ErhI | CCWWGG | 1 cut(s) | 115 |
| FspBI | CTAG | 1 cut(s) | 193 |
| HinfI | GANTC | 1 cut(s) | 120 |
| Hpy188III | TCNNGA | 1 cut(s) | 137 |
| HpyCH4III | ACNGT | 1 cut(s) | 149 |
| HpyCH4V | TGCA | 1 cut(s) | 179 |
| LmnI | GCTCC | 1 cut(s) | 117 |
| LpnPI | CCDG | 3 cut(s) | 150, 171, 189 |
| MaeI | CTAG | 1 cut(s) | 193 |
| MaeIII | GTNAC | 1 cut(s) | 149 |
| MboII | GAAGA | 3 cut(s) | 34, 37, 88 |
| MluCI | AATT | 1 cut(s) | 181 |
| MnlI | CCTC | 2 cut(s) | 10, 93 |
| MseI | TTAA | 2 cut(s) | 155, 168 |
| PfeI | GAWTC | 1 cut(s) | 120 |
| SaqAI | TTAA | 2 cut(s) | 155, 168 |
| SetI | ASST | 1 cut(s) | 114 |
| SfcI | CTRYAG | 1 cut(s) | 145 |
| SgeI | CNNG | 4 cut(s) | 44, 128, 149, 188 |
| Sse9I | AATT | 1 cut(s) | 181 |
| SspI | AATATT | 2 cut(s) | 128, 165 |
| SspMI | CTAG | 1 cut(s) | 193 |
| StyI | CCWWGG | 1 cut(s) | 115 |
| TaaI | ACNGT | 1 cut(s) | 149 |
| TasI | AATT | 1 cut(s) | 181 |
| TfiI | GAWTC | 1 cut(s) | 120 |
| Tru1I | TTAA | 2 cut(s) | 155, 168 |
| Tru9I | TTAA | 2 cut(s) | 155, 168 |
| TspDTI | ATGAA | 2 cut(s) | 17, 69 |
| XapI | RAATTY | 1 cut(s) | 181 |
| XspI | CTAG | 1 cut(s) | 193 |