RLG00000020258

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000193
Physical Location & Seq
Reverse (-)
62942708 .. 62947222
4515 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020258

Sequence Viewer

Length: 786 bp
ATGAAACAGAGGAGACTGTTAACTTACGTGTTGGACCTTAAAACTCAACTGGGTTATTTCAAGAATGTGGAGAAGCAGTTGAGGCACAGACTAGGTGAAGCAGAAGCACACACATTGTTGTCCGAAGCTGTTTACTTGATTGCCATCGGAAGCAATGATTACGTTGCCCCATTCATAACGAATTCAAGTTTGTTCGAGTCCCACTCACATGAAGAATATGTTGGCATGGTGACAGGAAACCTTACAAATGTGATCAAAGAAATATACAAGAAAGGAGGAAGAAAATTTGGGATTAAAGGCATAGGGAATTTGGGTTGTGTACCGAGCTTGAGAACGGTTAAACCAGCAAACACAAGCACCTGCAATGAAGAAATAAATGCAATTTCAAAACTCCACAATACGGTACTTGCTAAAGTCCTCCTGGAGCTGAAAGGACAGCTCCAAGACTTCATATACTCGAATCCAAATTTCTACTCTTACGCAAATGACGTAGTTCATAATCCATCAAAATACGGTTTCAAGGAAGGAAAGATGGCATGCTGTGGCTCTGGTCCATACAGAGGAATCAATAGCTGCGGAGGGAAGCGAGGTGTGACTGAGTATCAGTTATGTGACAATGTTACTGACTATGTATACTTTGATTCCGACCATCCCACAGAAAGGTGTAACCAGAAACTTTCCAAGTTATGGTGGAGCCATACTCCTGATGTCACAGCACGTTACATCAATTTGAAACCTGATGTCACAGCACGTTACATCAATTTGAAAGAGCTATTTGAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

262

Amino Acids

29.49

Weight (kDa)

8.81

Isoelectric Point (pI)

24.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 229 1.7e-12 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 368
Acc36I ACCTGC 1 cut(s) 368
AccB7I CCANNNNNTGG 1 cut(s) 687
AccI GTMKAC 1 cut(s) 633
AciI CCGC 1 cut(s) 576
AcsI RAATTY 4 cut(s) 181, 284, 307, 466
AfaI GTAC 2 cut(s) 321, 405
AfiI CCNNNNNNNGG 4 cut(s) 400, 560, 660, 687
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 7 cut(s) 61, 186, 387, 520, 733, 766, 779
AjnI CCWGG 1 cut(s) 420
AjuI GAANNNNNNNTTGG 2 cut(s) 204, 236
AluBI AGCT 6 cut(s) 128, 327, 427, 439, 573, 772
AluI AGCT 6 cut(s) 128, 327, 427, 439, 573, 772
Alw26I GTCTC 1 cut(s) 7
ApeKI GCWGC 1 cut(s) 573
ApoI RAATTY 4 cut(s) 181, 284, 307, 466
AspS9I GGNCC 2 cut(s) 34, 551
AsuHPI GGTGA 2 cut(s) 107, 241
AvaII GGWCC 2 cut(s) 34, 551
BbvI GCAGC 1 cut(s) 560
BccI CCATC 4 cut(s) 152, 511, 526, 657
BciT130I CCWGG 1 cut(s) 422
BclI TGATCA 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 7
BfaI CTAG 1 cut(s) 92
BfuAI ACCTGC 1 cut(s) 368
BisI GCNGC 1 cut(s) 574
BlsI GCNGC 1 cut(s) 575
Bme1390I CCNGG 1 cut(s) 422
Bme18I GGWCC 2 cut(s) 34, 551
BmgT120I GGNCC 2 cut(s) 34, 551
BmiI GGNNCC 1 cut(s) 695
BmrFI CCNGG 1 cut(s) 422
BmrI ACTGGG 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 59
BplI GAGNNNNNCTC 4 cut(s) 188, 220, 685, 717
BpmI CTGGAG 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 349
BsaAI YACGTR 1 cut(s) 28
BsaBI GATNNNNATC 1 cut(s) 143
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 4 cut(s) 400, 560, 660, 687
Bse1I ACTGG 1 cut(s) 54
Bse3DI GCAATG 2 cut(s) 160, 370
Bse8I GATNNNNATC 1 cut(s) 143
BseBI CCWGG 1 cut(s) 422
BseGI GGATG 1 cut(s) 649
BseJI GATNNNNATC 1 cut(s) 143
BseLI CCNNNNNNNGG 4 cut(s) 400, 560, 660, 687
BseMI GCAATG 2 cut(s) 160, 370
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 1 cut(s) 54
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 560
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 4 cut(s) 400, 560, 660, 687
BsmAI GTCTC 1 cut(s) 7
BsmFI GGGAC 1 cut(s) 184
Bsp143I GATC 1 cut(s) 252
BspACI CCGC 1 cut(s) 576
BspCNI CTCAG 1 cut(s) 589
BspLI GGNNCC 1 cut(s) 695
BspMI ACCTGC 1 cut(s) 368
BsrDI GCAATG 2 cut(s) 160, 370
BsrI ACTGG 1 cut(s) 54
BssMI GATC 1 cut(s) 252
BssNAI GTATAC 1 cut(s) 634
Bst1107I GTATAC 1 cut(s) 634
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 4 cut(s) 18, 337, 403, 515
BstBAI YACGTR 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 538
BstDEI CTNAG 1 cut(s) 597
BstF5I GGATG 1 cut(s) 649
BstKTI GATC 1 cut(s) 255
BstMAI GTCTC 1 cut(s) 7
BstMBI GATC 1 cut(s) 252
BstMWI GCNNNNNNNGC 1 cut(s) 82
BstNI CCWGG 1 cut(s) 422
BstNSI RCATGY 1 cut(s) 540
BstSCI CCNGG 1 cut(s) 420
BstV1I GCAGC 1 cut(s) 560
BstZ17I GTATAC 1 cut(s) 634
BtsCI GGATG 1 cut(s) 649
BveI ACCTGC 1 cut(s) 368
Cac8I GCNNGC 1 cut(s) 538
Cfr13I GGNCC 2 cut(s) 34, 551
Csp6I GTAC 2 cut(s) 320, 404
CviAII CATG 3 cut(s) 209, 226, 537
CviJI RGCY 8 cut(s) 128, 327, 427, 439, 546, 573, 696, 772
CviKI_1 RGCY 8 cut(s) 128, 327, 427, 439, 546, 573, 696, 772
CviQI GTAC 2 cut(s) 320, 404
DdeI CTNAG 1 cut(s) 597
DpnI GATC 1 cut(s) 254
DpnII GATC 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 34, 551
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 420
FaeI CATG 3 cut(s) 212, 229, 540
FaqI GGGAC 1 cut(s) 184
FatI CATG 3 cut(s) 208, 225, 536
FbaI TGATCA 1 cut(s) 252
FblI GTMKAC 1 cut(s) 633
Fnu4HI GCNGC 1 cut(s) 574
FokI GGATG 1 cut(s) 636
Fsp4HI GCNGC 1 cut(s) 574
FspBI CTAG 1 cut(s) 92
GluI GCNGC 1 cut(s) 574
GsuI CTGGAG 1 cut(s) 443
Hin1II CATG 3 cut(s) 212, 229, 540
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 4 cut(s) 197, 460, 564, 641
HpaI GTTAAC 1 cut(s) 21
HphI GGTGA 2 cut(s) 107, 241
Hpy166II GTNNAC 4 cut(s) 21, 133, 320, 634
Hpy188I TCNGA 3 cut(s) 124, 149, 646
Hpy188III TCNNGA 2 cut(s) 61, 704
Hpy8I GTNNAC 4 cut(s) 21, 133, 320, 634
HpyAV CCTTC 1 cut(s) 518
HpyCH4III ACNGT 4 cut(s) 18, 337, 403, 515
HpyCH4IV ACGT 5 cut(s) 27, 162, 489, 718, 751
HpyCH4V TGCA 2 cut(s) 363, 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 82
HpyF3I CTNAG 1 cut(s) 597
HpySE526I ACGT 5 cut(s) 27, 162, 489, 718, 751
Hsp92II CATG 3 cut(s) 212, 229, 540
Ksp22I TGATCA 1 cut(s) 252
KspAI GTTAAC 1 cut(s) 21
Kzo9I GATC 1 cut(s) 252
LmnI GCTCC 3 cut(s) 424, 444, 693
Lsp1109I GCAGC 1 cut(s) 560
MaeI CTAG 1 cut(s) 92
MaeII ACGT 5 cut(s) 27, 162, 489, 718, 751
MaeIII GTNAC 9 cut(s) 229, 592, 611, 619, 665, 709, 719, 742, 752
MalI GATC 1 cut(s) 254
MboI GATC 1 cut(s) 252
MboII GAAGA 3 cut(s) 224, 291, 380
MluCI AATT 7 cut(s) 181, 284, 307, 381, 466, 727, 760
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 2 cut(s) 12, 669
MnlI CCTC 7 cut(s) 3, 75, 269, 428, 554, 572, 581
MseI TTAA 4 cut(s) 20, 39, 294, 339
MslI CAYNNNNRTG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 422
MvaI CCWGG 1 cut(s) 422
MwoI GCNNNNNNNGC 1 cut(s) 82
NdeII GATC 1 cut(s) 252
NlaIII CATG 3 cut(s) 212, 229, 540
NlaIV GGNNCC 1 cut(s) 695
NmuCI GTSAC 5 cut(s) 229, 592, 611, 709, 742
NspI RCATGY 1 cut(s) 540
PaeI GCATGC 1 cut(s) 540
PaqCI CACCTGC 1 cut(s) 368
PcsI WCGNNNNNNNCGW 1 cut(s) 486
PfeI GAWTC 3 cut(s) 460, 564, 641
PflMI CCANNNNNTGG 1 cut(s) 687
PfoI TCCNGGA 1 cut(s) 420
PkrI GCNGC 1 cut(s) 575
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
Ppu21I YACGTR 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 420
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 1 cut(s) 695
PspPI GGNCC 2 cut(s) 34, 551
RsaI GTAC 2 cut(s) 321, 405
RsaNI GTAC 2 cut(s) 320, 404
RseI CAYNNNNRTG 1 cut(s) 207
SaqAI TTAA 4 cut(s) 20, 39, 294, 339
SatI GCNGC 1 cut(s) 574
Sau3AI GATC 1 cut(s) 252
Sau96I GGNCC 2 cut(s) 34, 551
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 1 cut(s) 422
SinI GGWCC 2 cut(s) 34, 551
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
SphI GCATGC 1 cut(s) 540
Sse9I AATT 7 cut(s) 181, 284, 307, 381, 466, 727, 760
SsiI CCGC 1 cut(s) 576
SspMI CTAG 1 cut(s) 92
StyD4I CCNGG 1 cut(s) 420
TaaI ACNGT 4 cut(s) 18, 337, 403, 515
TaiI ACGT 5 cut(s) 30, 165, 492, 721, 754
TaqI TCGA 2 cut(s) 195, 458
TasI AATT 7 cut(s) 181, 284, 307, 381, 466, 727, 760
TfiI GAWTC 3 cut(s) 460, 564, 641
Tru1I TTAA 4 cut(s) 20, 39, 294, 339
Tru9I TTAA 4 cut(s) 20, 39, 294, 339
TseFI GTSAC 5 cut(s) 229, 592, 611, 709, 742
TseI GCWGC 1 cut(s) 573
Tsp45I GTSAC 5 cut(s) 229, 592, 611, 709, 742
TspDTI ATGAA 6 cut(s) 17, 163, 225, 381, 439, 485
Van91I CCANNNNNTGG 1 cut(s) 687
VpaK11BI GGWCC 2 cut(s) 34, 551
XapI RAATTY 4 cut(s) 181, 284, 307, 466
XceI RCATGY 1 cut(s) 540
XmiI GTMKAC 1 cut(s) 633
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.