Rw7G033400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000193
Physical Location & Seq
Forward (+)
49182844 .. 49184731
1888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G033400.1

Sequence Viewer

Length: 1149 bp
ATGGCAACTTCTTCAAGATTTCAAGTATATGTCCTGGCTTTTTGTGCAACCCTTCTTATTCAAAGTGGCTGTTATGGCTATTCTGTGCATCAGAGGAAACATGCAGCCTTGTTCATCTTCGGAGGTTCAACATTTGATGTTGGAAATAATAACTACATAAACACTTACAATTACTTTAAAGCAAATTTCTTCCCATATGGGGAAACCTTCTTCAGCCACCCGACTGGTAGGTTCTCCGATGGTCGTCTAATCTCAGATATCATTGCTGAATATGCAGACTTGCCAATGATTCCACCATACTTACAACCGGGTTTTGACAACTATACTAATGGGGTGAACTTTGCATCTGCTGGGGCTGGTGCTCTAGCTGAAACTAATCAAGGATTTGCGTTGGACCTTAAAACTCAACTGGGTTATTTCAAGAATGTGGAGAAGCAGTTGAGGCACAGACTAGGTGAAGCAGAAGCTCACACATTGTTGTCCCAAGCTGTTTACTTGATTGCCATTGGAACCAATGATTACCTTGCATTCATAGCGAATTCAAGTTTGTCCGAGTCTCGCTCACATGAAGAATATGTTGGCATGGTGACAGGAAACATTACAAATGTGATCAAAGAAATATACAAGAAAGGAGGAAGAAAATTTGGGATTAAAGGCATAGAGAATTTGGGTTGTACCCCGGGCTTGAGAACAGTTAGACCAGGAAACACAAGCACCTGTAATGAAGAAGTAAATGCAATTTCAAAACTCCACAATAGAGTACTTGCTAAAGTCCTCGAGGAGCTGAAAGGACAGCTCCAAGACTTCATATACTCGAATCCAAATTTCTACTCTTACGCAAATGACGTAGTTAATAATCCATCAAAACATGGTTTCAAGGAAGGAAAGATGGCATGCTGTGGCTCTGGTCCATACAGAGGAAATAATACCTGCGGAGGGAAGCGAGGTATAACTGAGTATCAGTTATGTGACAATGTTACTGACTATGTCTACTTTGATTCCGGTCATCCCACAGAAAGGTATAACCAGAAACTTTCCAAGTTATGGTGGAGCCATACTCCCGATGTCACAGCACGTTACATCAATTTGAAAGAGCTATTTGAAGTATCGAATAAGAATTCGGATACATCTTATGTTTCAGTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

382

Amino Acids

42.59

Weight (kDa)

8.0

Isoelectric Point (pI)

22.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 348 2.7e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 776
Acc36I ACCTGC 1 cut(s) 938
AccB7I CCANNNNNTGG 1 cut(s) 1044
AccI GTMKAC 1 cut(s) 990
AciI CCGC 1 cut(s) 933
AcsI RAATTY 6 cut(s) 184, 538, 641, 664, 823, 1117
AcuI CTGAAG 1 cut(s) 196
AfaI GTAC 2 cut(s) 676, 762
AfiI CCNNNNNNNGG 5 cut(s) 199, 917, 936, 1017, 1044
AjnI CCWGG 2 cut(s) 33, 700
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AluBI AGCT 6 cut(s) 368, 467, 488, 784, 796, 1096
AluI AGCT 6 cut(s) 368, 467, 488, 784, 796, 1096
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 1 cut(s) 561
Ama87I CYCGRG 2 cut(s) 679, 776
ApeKI GCWGC 1 cut(s) 104
ApoI RAATTY 6 cut(s) 184, 538, 641, 664, 823, 1117
AspS9I GGNCC 2 cut(s) 394, 908
AsuC2I CCSGG 3 cut(s) 309, 680, 681
AsuHPI GGTGA 3 cut(s) 346, 467, 598
AvaI CYCGRG 2 cut(s) 679, 776
AvaII GGWCC 2 cut(s) 394, 908
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 1 cut(s) 116
BccI CCATC 3 cut(s) 233, 868, 883
BciT130I CCWGG 2 cut(s) 35, 702
BciVI GTATCC 1 cut(s) 1117
BclI TGATCA 1 cut(s) 609
BcnI CCSGG 3 cut(s) 309, 680, 681
BcoDI GTCTC 1 cut(s) 561
BfaI CTAG 2 cut(s) 365, 452
BfuAI ACCTGC 1 cut(s) 938
BfuI GTATCC 1 cut(s) 1117
BisI GCNGC 1 cut(s) 105
BlsI GCNGC 1 cut(s) 106
BmcAI AGTACT 1 cut(s) 762
Bme1390I CCNGG 5 cut(s) 35, 309, 680, 681, 702
Bme18I GGWCC 2 cut(s) 394, 908
BmeT110I CYCGRG 2 cut(s) 679, 776
BmgT120I GGNCC 2 cut(s) 394, 908
BmiI GGNNCC 2 cut(s) 511, 1052
BmrFI CCNGG 5 cut(s) 35, 309, 680, 681, 702
BmrI ACTGGG 1 cut(s) 419
BmsI GCATC 2 cut(s) 97, 353
BmuI ACTGGG 1 cut(s) 419
BplI GAGNNNNNCTC 4 cut(s) 545, 577, 1042, 1074
BpuEI CTTGAG 1 cut(s) 706
BpuMI CCSGG 3 cut(s) 309, 680, 681
BsaJI CCNNGG 2 cut(s) 678, 679
BsaWI WCCGGW 1 cut(s) 1001
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 5 cut(s) 199, 917, 936, 1017, 1044
Bse1I ACTGG 2 cut(s) 229, 414
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 2 cut(s) 35, 702
BseDI CCNNGG 2 cut(s) 678, 679
BseGI GGATG 1 cut(s) 1006
BseLI CCNNNNNNNGG 5 cut(s) 199, 917, 936, 1017, 1044
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 267, 945
BseNI ACTGG 2 cut(s) 229, 414
BseRI GAGGAG 1 cut(s) 794
BseXI GCAGC 1 cut(s) 116
BseYI CCCAGC 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 364
BsiHKCI CYCGRG 2 cut(s) 679, 776
BsiSI CCGG 3 cut(s) 308, 680, 1002
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 5 cut(s) 199, 917, 936, 1017, 1044
BsmAI GTCTC 1 cut(s) 561
BsmFI GGGAC 1 cut(s) 466
BsmI GAATGC 1 cut(s) 527
BsoBI CYCGRG 2 cut(s) 679, 776
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 1 cut(s) 609
BspACI CCGC 1 cut(s) 933
BspCNI CTCAG 2 cut(s) 266, 946
BspLI GGNNCC 2 cut(s) 511, 1052
BspMI ACCTGC 1 cut(s) 938
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 2 cut(s) 229, 414
BssECI CCNNGG 2 cut(s) 678, 679
BssMI GATC 1 cut(s) 609
Bst2UI CCWGG 2 cut(s) 35, 702
Bst4CI ACNGT 1 cut(s) 694
BstC8I GCNNGC 1 cut(s) 895
BstDEI CTNAG 2 cut(s) 253, 954
BstF5I GGATG 1 cut(s) 1006
BstKTI GATC 1 cut(s) 612
BstMAI GTCTC 1 cut(s) 561
BstMBI GATC 1 cut(s) 609
BstMWI GCNNNNNNNGC 5 cut(s) 44, 75, 272, 442, 533
BstNI CCWGG 2 cut(s) 35, 702
BstNSI RCATGY 2 cut(s) 104, 897
BstSCI CCNGG 5 cut(s) 33, 307, 678, 679, 700
BstV1I GCAGC 1 cut(s) 116
BstXI CCANNNNNNTGG 1 cut(s) 224
BsuI GTATCC 1 cut(s) 1117
BtsCI GGATG 1 cut(s) 1006
BveI ACCTGC 1 cut(s) 938
Cac8I GCNNGC 1 cut(s) 895
Cfr13I GGNCC 2 cut(s) 394, 908
Cfr9I CCCGGG 1 cut(s) 679
Csp6I GTAC 2 cut(s) 675, 761
CviAII CATG 5 cut(s) 101, 566, 583, 869, 894
CviQI GTAC 2 cut(s) 675, 761
DdeI CTNAG 2 cut(s) 253, 954
DpnI GATC 1 cut(s) 611
DpnII GATC 1 cut(s) 609
DraI TTTAAA 1 cut(s) 178
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 394, 908
Eco57I CTGAAG 1 cut(s) 196
Eco88I CYCGRG 2 cut(s) 679, 776
EcoRI GAATTC 2 cut(s) 538, 1117
EcoRII CCWGG 2 cut(s) 33, 700
EcoRV GATATC 1 cut(s) 259
FaeI CATG 5 cut(s) 104, 569, 586, 872, 897
FaqI GGGAC 1 cut(s) 466
FatI CATG 5 cut(s) 100, 565, 582, 868, 893
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 609
FblI GTMKAC 1 cut(s) 990
Fnu4HI GCNGC 1 cut(s) 105
FokI GGATG 1 cut(s) 993
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 2 cut(s) 365, 452
GluI GCNGC 1 cut(s) 105
GsaI CCCAGC 1 cut(s) 354
HapII CCGG 3 cut(s) 308, 680, 1002
Hin1II CATG 5 cut(s) 104, 569, 586, 872, 897
HinfI GANTC 4 cut(s) 289, 554, 817, 998
HpaII CCGG 3 cut(s) 308, 680, 1002
HphI GGTGA 3 cut(s) 346, 467, 598
Hpy166II GTNNAC 3 cut(s) 337, 493, 991
Hpy188I TCNGA 6 cut(s) 93, 122, 238, 256, 553, 1123
Hpy188III TCNNGA 3 cut(s) 15, 421, 1061
Hpy8I GTNNAC 3 cut(s) 337, 493, 991
HpyAV CCTTC 3 cut(s) 62, 217, 875
HpyCH4III ACNGT 1 cut(s) 694
HpyCH4IV ACGT 2 cut(s) 846, 1075
HpyCH4V TGCA 7 cut(s) 47, 88, 104, 275, 344, 527, 737
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 75, 272, 442, 533
HpyF3I CTNAG 2 cut(s) 253, 954
HpySE526I ACGT 2 cut(s) 846, 1075
Hsp92II CATG 5 cut(s) 104, 569, 586, 872, 897
Ksp22I TGATCA 1 cut(s) 609
Kzo9I GATC 1 cut(s) 609
LmnI GCTCC 3 cut(s) 781, 801, 1050
Lsp1109I GCAGC 1 cut(s) 116
LweI GCATC 2 cut(s) 97, 353
MaeI CTAG 2 cut(s) 365, 452
MaeII ACGT 2 cut(s) 846, 1075
MaeIII GTNAC 5 cut(s) 586, 968, 976, 1066, 1076
MalI GATC 1 cut(s) 611
MboI GATC 1 cut(s) 609
MboII GAAGA 7 cut(s) 3, 109, 181, 202, 581, 648, 737
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 9 cut(s) 169, 184, 538, 641, 664, 738, 823, 1084, 1117
MlyI GAGTC 1 cut(s) 563
MmeI TCCRAC 2 cut(s) 121, 372
MnlI CCTC 9 cut(s) 87, 116, 435, 626, 772, 785, 911, 929, 938
MseI TTAA 4 cut(s) 177, 399, 651, 852
MspI CCGG 3 cut(s) 308, 680, 1002
MspR9I CCNGG 5 cut(s) 35, 309, 680, 681, 702
Mva1269I GAATGC 1 cut(s) 527
MvaI CCWGG 2 cut(s) 35, 702
MwoI GCNNNNNNNGC 5 cut(s) 44, 75, 272, 442, 533
NciI CCSGG 3 cut(s) 309, 680, 681
NdeI CATATG 1 cut(s) 196
NdeII GATC 1 cut(s) 609
NlaIII CATG 5 cut(s) 104, 569, 586, 872, 897
NlaIV GGNNCC 2 cut(s) 511, 1052
NmuCI GTSAC 3 cut(s) 586, 968, 1066
NspI RCATGY 2 cut(s) 104, 897
PaeI GCATGC 1 cut(s) 897
PaeR7I CTCGAG 1 cut(s) 776
PcsI WCGNNNNNNNCGW 1 cut(s) 843
PctI GAATGC 1 cut(s) 527
PfeI GAWTC 3 cut(s) 289, 817, 998
PflFI GACNNNGTC 1 cut(s) 986
PflMI CCANNNNNTGG 1 cut(s) 1044
PkrI GCNGC 1 cut(s) 106
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
Psp6I CCWGG 2 cut(s) 33, 700
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 2 cut(s) 33, 700
PspN4I GGNNCC 2 cut(s) 511, 1052
PspPI GGNCC 2 cut(s) 394, 908
PspXI VCTCGAGB 1 cut(s) 776
PsyI GACNNNGTC 1 cut(s) 986
RsaI GTAC 2 cut(s) 676, 762
RsaNI GTAC 2 cut(s) 675, 761
SaqAI TTAA 4 cut(s) 177, 399, 651, 852
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 1 cut(s) 609
Sau96I GGNCC 2 cut(s) 394, 908
ScaI AGTACT 1 cut(s) 762
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 5 cut(s) 35, 309, 680, 681, 702
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 2 cut(s) 97, 353
Sfr274I CTCGAG 1 cut(s) 776
SinI GGWCC 2 cut(s) 394, 908
SlaI CTCGAG 1 cut(s) 776
SmaI CCCGGG 1 cut(s) 681
SmlI CTYRAG 2 cut(s) 685, 776
SmoI CTYRAG 2 cut(s) 685, 776
SphI GCATGC 1 cut(s) 897
Sse9I AATT 9 cut(s) 169, 184, 538, 641, 664, 738, 823, 1084, 1117
SsiI CCGC 1 cut(s) 933
SspMI CTAG 2 cut(s) 365, 452
StyD4I CCNGG 5 cut(s) 33, 307, 678, 679, 700
TaaI ACNGT 1 cut(s) 694
TaiI ACGT 2 cut(s) 849, 1078
TaqI TCGA 3 cut(s) 777, 815, 1109
TasI AATT 9 cut(s) 169, 184, 538, 641, 664, 738, 823, 1084, 1117
TatI WGTACW 1 cut(s) 760
TfiI GAWTC 3 cut(s) 289, 817, 998
Tru1I TTAA 4 cut(s) 177, 399, 651, 852
Tru9I TTAA 4 cut(s) 177, 399, 651, 852
TseFI GTSAC 3 cut(s) 586, 968, 1066
TseI GCWGC 1 cut(s) 104
Tsp45I GTSAC 3 cut(s) 586, 968, 1066
TspDTI ATGAA 5 cut(s) 103, 520, 582, 738, 796
TspMI CCCGGG 1 cut(s) 679
Tth111I GACNNNGTC 1 cut(s) 986
Van91I CCANNNNNTGG 1 cut(s) 1044
VpaK11BI GGWCC 2 cut(s) 394, 908
XapI RAATTY 6 cut(s) 184, 538, 641, 664, 823, 1117
XceI RCATGY 2 cut(s) 104, 897
XhoI CTCGAG 1 cut(s) 776
XmaI CCCGGG 1 cut(s) 679
XmiI GTMKAC 1 cut(s) 990
XspI CTAG 2 cut(s) 365, 452
ZrmI AGTACT 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.