Rh2AG457200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000193
Physical Location & Seq
Reverse (-)
67227692 .. 67229231
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG457200.1

Sequence Viewer

Length: 1113 bp
ATGTCGACTTCACTGTTTCAAGTATGTATGATCTTTGCTTTTTGTGCAAGTCTTCTTACTCCAAGCAGCTGCCATGACAACTCAAGGCTTCACAAGAAACACGCAGCCTTGTTCGTCTTTGGTGATTCGCAGTTTGATGTCGGAAATAATAACTACTTGAACACTTCCACTGAACTTCAAGCAAATTTCTGGCCATATGGGGAAACATTCTTCAAGCACCCCACTGGTAGATTTTCGGATGGACGTCTAATCCCTGATTTTATTGCTGAGTATGCAAAGTTGCCACTTATTCCACCATATTTACGACGTGGGTTCAAAAGCTATACTTATGGGGTCAACTTCGCATCTGCCGGGGCTGGTGCTCTAGCTGAATCTTATCAAGGATTTGTTATAGACCTTAAAACTCAACTGGGTTATTTCAAGAAAGTGGAGAAGCAGTTGAGGCACAAACTAGGCCATGCAGAAACCTACACATTGCTGTCAGAAGCTGTATACTTAATTGGCATTGGAGCGAATGACTATTTTTATGCACTCGGCACAAATTCCAGTTTGTTTGAGTCCTCACCTGAAGAATTTGTTGGCCTGGTGATAGGAAACCTGACAACTGTGATCAAGGAAATATACAAGAAAGGAGGAAGGAAATTTGGGTTTACAAGCATGGCTCCTCTGGGTTGTGTACCAAGCATGAGAGCAATGAAACCAGGAAACACAGGCACATGTGTGGAAGAAGTGAATGCGGTAGCGAAACTACACAACAGAGTACTTGATAAAGTCCTCCTAAAGCTTAAAGGACATCTCCTAGGATTCAAGTATTCATATCCAAATGTATACCCTCACATAGACGAAATATTTAACAATGCATCTAAACATGGTTTCAAGGAGGGAAAGACGGCATGTTGCGGATCTGGTCCATACAGAGGAATTGGGAGTTGTGGAGGGAAGCGAGGCGTGACAGTGTATGAACTATGTGACAATGTTACTGAATATGTGTTTTTCGACTCCAATCATCCCACAGAAAGGTTTTACCAGAAAGTTTCCAAGTTATGGTGGAGCCATACCCCTAAACAGACTGAGACTTACATTGGTCTGAAAGAGCTATTTGAAGTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

370

Amino Acids

41.42

Weight (kDa)

8.85

Isoelectric Point (pI)

29.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 348 5.8e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 247
AccB7I CCANNNNNTGG 1 cut(s) 1044
AccI GTMKAC 3 cut(s) 5, 492, 828
AciI CCGC 2 cut(s) 737, 900
AclWI GGATC 1 cut(s) 910
AcoI YGGCCR 1 cut(s) 191
AcsI RAATTY 4 cut(s) 184, 541, 572, 641
AcuI CTGAAG 1 cut(s) 588
AcyI GRCGYC 1 cut(s) 244
AfaI GTAC 2 cut(s) 678, 762
AfiI CCNNNNNNNGG 3 cut(s) 917, 1017, 1044
AflIII ACRYGT 1 cut(s) 716
AgsI TTSAA 9 cut(s) 20, 160, 179, 214, 316, 421, 808, 877, 1103
AjiI CACGTC 1 cut(s) 308
AjnI CCWGG 2 cut(s) 582, 700
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AleI CACNNNNGTG 1 cut(s) 719
AluBI AGCT 6 cut(s) 69, 321, 368, 488, 784, 1096
AluI AGCT 6 cut(s) 69, 321, 368, 488, 784, 1096
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 1 cut(s) 1067
AlwI GGATC 1 cut(s) 910
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 3 cut(s) 191, 454, 580
ApeKI GCWGC 3 cut(s) 66, 69, 104
ApoI RAATTY 4 cut(s) 184, 541, 572, 641
AspA2I CCTAGG 1 cut(s) 799
AspS9I GGNCC 1 cut(s) 908
AsuC2I CCSGG 1 cut(s) 352
AsuHPI GGTGA 3 cut(s) 134, 555, 598
AvaII GGWCC 1 cut(s) 908
AvrII CCTAGG 1 cut(s) 799
BalI TGGCCA 1 cut(s) 193
BbsI GAAGAC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 3 cut(s) 56, 78, 116
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 906
BciT130I CCWGG 2 cut(s) 584, 702
BclI TGATCA 1 cut(s) 609
BcnI CCSGG 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 1067
BfaI CTAG 3 cut(s) 365, 452, 800
BisI GCNGC 3 cut(s) 67, 70, 105
BlnI CCTAGG 1 cut(s) 799
BlsI GCNGC 3 cut(s) 68, 71, 106
BmcAI AGTACT 1 cut(s) 762
Bme1390I CCNGG 3 cut(s) 352, 584, 702
Bme18I GGWCC 1 cut(s) 908
BmgBI CACGTC 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 908
BmiI GGNNCC 2 cut(s) 663, 1052
BmrFI CCNGG 3 cut(s) 352, 584, 702
BmrI ACTGGG 1 cut(s) 419
BmsI GCATC 2 cut(s) 353, 869
BmuI ACTGGG 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 67
BpuMI CCSGG 1 cut(s) 352
BsaHI GRCGYC 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 351, 799
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 3 cut(s) 917, 1017, 1044
Bse1I ACTGG 3 cut(s) 229, 414, 546
Bse3DI GCAATG 2 cut(s) 473, 699
BseBI CCWGG 2 cut(s) 584, 702
BseDI CCNNGG 2 cut(s) 351, 799
BseGI GGATG 2 cut(s) 244, 1006
BseLI CCNNNNNNNGG 3 cut(s) 917, 1017, 1044
BseMI GCAATG 2 cut(s) 473, 699
BseMII CTCAG 2 cut(s) 258, 1062
BseNI ACTGG 3 cut(s) 229, 414, 546
BseRI GAGGAG 1 cut(s) 654
BseXI GCAGC 3 cut(s) 56, 78, 116
BshFI GGCC 3 cut(s) 193, 456, 582
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 1 cut(s) 351
BslI CCNNNNNNNGG 3 cut(s) 917, 1017, 1044
BsmAI GTCTC 1 cut(s) 1067
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 3 cut(s) 193, 456, 582
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 3 cut(s) 30, 609, 902
BspACI CCGC 2 cut(s) 737, 900
BspANI GGCC 3 cut(s) 193, 456, 582
BspCNI CTCAG 2 cut(s) 259, 1063
BspLI GGNNCC 2 cut(s) 663, 1052
BspPI GGATC 1 cut(s) 910
BsrDI GCAATG 2 cut(s) 473, 699
BsrI ACTGG 3 cut(s) 229, 414, 546
BssECI CCNNGG 2 cut(s) 351, 799
BssMI GATC 3 cut(s) 30, 609, 902
BssNAI GTATAC 2 cut(s) 493, 829
BssNI GRCGYC 1 cut(s) 244
BssT1I CCWWGG 1 cut(s) 799
Bst1107I GTATAC 2 cut(s) 493, 829
Bst2UI CCWGG 2 cut(s) 584, 702
Bst4CI ACNGT 3 cut(s) 15, 607, 955
BstACI GRCGYC 1 cut(s) 244
BstDEI CTNAG 2 cut(s) 267, 1071
BstF5I GGATG 2 cut(s) 244, 1006
BstKTI GATC 3 cut(s) 33, 612, 905
BstMAI GTCTC 1 cut(s) 1067
BstMBI GATC 3 cut(s) 30, 609, 902
BstMWI GCNNNNNNNGC 3 cut(s) 44, 272, 442
BstNI CCWGG 2 cut(s) 584, 702
BstNSI RCATGY 2 cut(s) 720, 897
BstSCI CCNGG 3 cut(s) 350, 582, 700
BstV1I GCAGC 3 cut(s) 56, 78, 116
BstV2I GAAGAC 1 cut(s) 44
BstX2I RGATCY 1 cut(s) 902
BstYI RGATCY 1 cut(s) 902
BstZ17I GTATAC 2 cut(s) 493, 829
BsuRI GGCC 3 cut(s) 193, 456, 582
BtrI CACGTC 1 cut(s) 308
BtsCI GGATG 2 cut(s) 244, 1006
BtsIMutI CAGTG 4 cut(s) 11, 168, 222, 960
CaiI CAGNNNCTG 1 cut(s) 488
Cfr13I GGNCC 1 cut(s) 908
Csp6I GTAC 2 cut(s) 677, 761
CviAII CATG 7 cut(s) 74, 458, 658, 685, 717, 869, 894
CviQI GTAC 2 cut(s) 677, 761
DdeI CTNAG 2 cut(s) 267, 1071
DpnI GATC 3 cut(s) 32, 611, 904
DpnII GATC 3 cut(s) 30, 609, 902
EaeI YGGCCR 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 799
Eco47I GGWCC 1 cut(s) 908
Eco57I CTGAAG 1 cut(s) 588
EcoRII CCWGG 2 cut(s) 582, 700
EcoT14I CCWWGG 1 cut(s) 799
EcoT22I ATGCAT 1 cut(s) 862
ErhI CCWWGG 1 cut(s) 799
FaeI CATG 7 cut(s) 77, 461, 661, 688, 720, 872, 897
FalI AAGNNNNNCTT 2 cut(s) 310, 342
FatI CATG 7 cut(s) 73, 457, 657, 684, 716, 868, 893
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 609
FblI GTMKAC 3 cut(s) 5, 492, 828
Fnu4HI GCNGC 3 cut(s) 67, 70, 105
FokI GGATG 2 cut(s) 251, 993
Fsp4HI GCNGC 3 cut(s) 67, 70, 105
FspBI CTAG 3 cut(s) 365, 452, 800
GluI GCNGC 3 cut(s) 67, 70, 105
HaeIII GGCC 3 cut(s) 193, 456, 582
HapII CCGG 1 cut(s) 351
Hin1I GRCGYC 1 cut(s) 244
Hin1II CATG 7 cut(s) 77, 461, 661, 688, 720, 872, 897
HincII GTYRAC 2 cut(s) 6, 337
HindII GTYRAC 2 cut(s) 6, 337
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 5 cut(s) 125, 371, 557, 804, 998
HpaII CCGG 1 cut(s) 351
HphI GGTGA 3 cut(s) 134, 555, 598
Hpy166II GTNNAC 6 cut(s) 6, 337, 493, 651, 677, 829
Hpy188I TCNGA 4 cut(s) 143, 238, 484, 1089
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 6 cut(s) 6, 337, 493, 651, 677, 829
Hpy99I CGWCG 1 cut(s) 309
HpyAV CCTTC 1 cut(s) 630
HpyCH4III ACNGT 3 cut(s) 15, 607, 955
HpyCH4IV ACGT 2 cut(s) 244, 307
HpyCH4V TGCA 5 cut(s) 47, 275, 461, 530, 860
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 272, 442
HpyF3I CTNAG 2 cut(s) 267, 1071
HpySE526I ACGT 2 cut(s) 244, 307
Hsp92I GRCGYC 1 cut(s) 244
Hsp92II CATG 7 cut(s) 77, 461, 661, 688, 720, 872, 897
Ksp22I TGATCA 1 cut(s) 609
Kzo9I GATC 3 cut(s) 30, 609, 902
LmnI GCTCC 3 cut(s) 509, 667, 1050
Lsp1109I GCAGC 3 cut(s) 56, 78, 116
LweI GCATC 2 cut(s) 353, 869
MaeI CTAG 3 cut(s) 365, 452, 800
MaeII ACGT 2 cut(s) 244, 307
MaeIII GTNAC 3 cut(s) 949, 968, 976
MalI GATC 3 cut(s) 32, 611, 904
MboI GATC 3 cut(s) 30, 609, 902
MboII GAAGA 4 cut(s) 44, 202, 581, 737
MflI RGATCY 1 cut(s) 902
MhlI GDGCHC 1 cut(s) 364
MlsI TGGCCA 1 cut(s) 193
MluCI AATT 6 cut(s) 184, 498, 541, 572, 641, 921
MluNI TGGCCA 1 cut(s) 193
MlyI GAGTC 2 cut(s) 566, 992
MmeI TCCRAC 1 cut(s) 121
Mox20I TGGCCA 1 cut(s) 193
Mph1103I ATGCAT 1 cut(s) 862
MscI TGGCCA 1 cut(s) 193
MseI TTAA 4 cut(s) 399, 497, 786, 852
MslI CAYNNNNRTG 1 cut(s) 719
Msp20I TGGCCA 1 cut(s) 193
MspA1I CMGCKG 1 cut(s) 69
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 3 cut(s) 352, 584, 702
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 2 cut(s) 584, 702
MwoI GCNNNNNNNGC 3 cut(s) 44, 272, 442
NciI CCSGG 1 cut(s) 352
NdeI CATATG 1 cut(s) 196
NdeII GATC 3 cut(s) 30, 609, 902
NlaIII CATG 7 cut(s) 77, 461, 661, 688, 720, 872, 897
NlaIV GGNNCC 2 cut(s) 663, 1052
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 2 cut(s) 949, 968
NsiI ATGCAT 1 cut(s) 862
NspI RCATGY 2 cut(s) 720, 897
OliI CACNNNNGTG 1 cut(s) 719
PciI ACATGT 1 cut(s) 716
PctI GAATGC 1 cut(s) 739
PfeI GAWTC 3 cut(s) 125, 371, 804
PflMI CCANNNNNTGG 1 cut(s) 1044
PkrI GCNGC 3 cut(s) 68, 71, 106
PleI GAGTC 2 cut(s) 565, 992
PpsI GAGTC 2 cut(s) 565, 992
PscI ACATGT 1 cut(s) 716
Psp6I CCWGG 2 cut(s) 582, 700
PspGI CCWGG 2 cut(s) 582, 700
PspN4I GGNNCC 2 cut(s) 663, 1052
PspPI GGNCC 1 cut(s) 908
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 1 cut(s) 902
PvuII CAGCTG 1 cut(s) 69
RsaI GTAC 2 cut(s) 678, 762
RsaNI GTAC 2 cut(s) 677, 761
RseI CAYNNNNRTG 1 cut(s) 719
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 4 cut(s) 399, 497, 786, 852
SatI GCNGC 3 cut(s) 67, 70, 105
Sau3AI GATC 3 cut(s) 30, 609, 902
Sau96I GGNCC 1 cut(s) 908
ScaI AGTACT 1 cut(s) 762
SchI GAGTC 2 cut(s) 566, 992
ScrFI CCNGG 3 cut(s) 352, 584, 702
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 2 cut(s) 353, 869
SinI GGWCC 1 cut(s) 908
SmiMI CAYNNNNRTG 1 cut(s) 719
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 6 cut(s) 184, 498, 541, 572, 641, 921
SsiI CCGC 2 cut(s) 737, 900
SspI AATATT 1 cut(s) 849
SspMI CTAG 3 cut(s) 365, 452, 800
StyD4I CCNGG 3 cut(s) 350, 582, 700
StyI CCWWGG 1 cut(s) 799
TaaI ACNGT 3 cut(s) 15, 607, 955
TaiI ACGT 2 cut(s) 247, 310
TaqI TCGA 2 cut(s) 5, 996
TasI AATT 6 cut(s) 184, 498, 541, 572, 641, 921
TatI WGTACW 1 cut(s) 760
TfiI GAWTC 3 cut(s) 125, 371, 804
Tru1I TTAA 4 cut(s) 399, 497, 786, 852
Tru9I TTAA 4 cut(s) 399, 497, 786, 852
TscAI CASTG 4 cut(s) 18, 175, 229, 960
TseFI GTSAC 2 cut(s) 949, 968
TseI GCWGC 3 cut(s) 66, 69, 104
Tsp45I GTSAC 2 cut(s) 949, 968
TspDTI ATGAA 3 cut(s) 710, 804, 975
TspRI CASTG 4 cut(s) 18, 175, 229, 960
Van91I CCANNNNNTGG 1 cut(s) 1044
VpaK11BI GGWCC 1 cut(s) 908
XapI RAATTY 4 cut(s) 184, 541, 572, 641
XceI RCATGY 2 cut(s) 720, 897
XmaJI CCTAGG 1 cut(s) 799
XmiI GTMKAC 3 cut(s) 5, 492, 828
XspI CTAG 3 cut(s) 365, 452, 800
ZraI GACGTC 1 cut(s) 245
ZrmI AGTACT 1 cut(s) 762
Zsp2I ATGCAT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.