Rorug02G0398800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000193
Physical Location & Seq
Forward (+)
50910484 .. 50915427
4944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0398800.1

Sequence Viewer

Length: 1662 bp
ATGTCGGCGCCGGCCCCGTCCACCACCAACTCCACCTCACCTACTCCCCCTCCGCCGGCCGACGCTACTGCTCCTACCACTACTCCTCCGACCACCAACACTACCGCGTCGCCGCCAACCAACTCCACCACATCGCCGCCACCTCCGGCATCGACATCTAATTCGCCACCGACCATAGGGCAGTTGCCGTCGGGGAGCACGCCGGTCGGGGTTATAGTCGGAGTTGCGATAGGAGGGTTCAGTGTGCTTGTTGTAGTGGCCATTATCTTCTTTGTCTATCGGAGACGGAAGAACAGGTTGGCGAGAGCTAATGAGTCAGGTCAACAACCCAAAGATGACCAATTAAAAGGCCCACCTCAAAACTTGCAACAGAACTATGCTCCTCCTTCAGCAGACAAAGTAGAAATGTTTCCAATGCCTACTCATTCCCGTAAACCATCTTCAACCGGTAGCGGCTATGGATCAGATAAACCGCTCCCAGCCTTTGGATTTTCCGAAAGCACATTTACATATAAAGAGTTAGACATGGCAACAAATGGTTTCTCCAACGACAATCTTCTTGGTCAAGGAGGCTTTGGTTATGTCCATAAAGGAATTCTTTCAAATGGGAAAGTGGTTGCAATTAAACAGCTGAAAGCTGGGAGTGGACAGGGGGAGCGTGAATTCCAGGCAGAAGTTGAAGTCATTAATCGTGTCCATCACAGACATCTTGTATCACTGGTTGGATACTGCATTAGTGGGGCCCAGAGATTGCTTGTTTATGAGTTCGTTTCCAACAGCACCTTGGAATTTCACTTGCATGGAAAGGGGCGACCAACTATGACTTGGCCAACAAGAATGAAAATTGCTGTAGGATCTGCAAAAGGATTGGCATATCTCCATGAGGACTGTCATCCCAAGATCATACACCGTGACATCAAGTCTGCCAATATTCTTATTGATGACAACTTTGAGGCAAAGGTTGCAGACTTTGGACTTGCCAAGTTGGCCTTAGATACAGATACTCATGTCTCCACAAGGGTAATGGGAACTTTTGGATACTTAGCTCCTGAATATGCATCCAGTGGAAAGCTCAGTGATAAGTCAGATGTCTTCTCATTCGGGGTCGTGCTATTGGAATTGATTACTGGACGCCAACCCATTGATAAAACTCATTCCTTCACTGATGATAGCATGGTTGAGTGGGCAAGGCCTTTGCTCGCGCGAGCATTGGAAAAGGGAAACTTTGATGGTCTTGTTGATGAAAGGTTGCAGAATGATTACAACTCCAGCGAAATGGCTCGTATGATTGCCTGCGCTGCTGCTTCTGTGCGTCATTCTGCCCGCCGTCGGCCAAAAATGAGCCAGGTAGTTCGAGCTTTGGAAGGCAATCTTTCTCCAGATGAATTAAATGAAGGAGTTATACCAGGTCAAAGTATGATATACAGTTCCTCTGAAAGCACACAATACAGCACTCGTGAATACAAGGAAGACATGTTGAAATTTAGAAAGTTAGCACTAGAAAGCCAAGAGCTAGATCAAGGAATTAGTGAGACCAGTGGGCCCAGCAGTGATTTTGGCCAGCACCAATCTGCCTCTAGTGGTGAAGGCCAACAAACCAGTCAAGATATCGAACACGGGCAAATGAACAAAGACAGCCAAGACTATGGTAAAAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

553

Amino Acids

59.74

Weight (kDa)

6.44

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 182 - 388 2.4e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 184 - 452 1.7e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AccB7I CCANNNNNTGG 1 cut(s) 485
AccBSI CCGCTC 1 cut(s) 475
AccII CGCG 3 cut(s) 107, 1202, 1204
AciI CCGC 7 cut(s) 53, 105, 113, 137, 453, 473, 1324
AclWI GGATC 2 cut(s) 469, 862
AcoI YGGCCR 5 cut(s) 57, 258, 827, 1331, 1558
AcsI RAATTY 4 cut(s) 594, 662, 788, 1481
AcuI CTGAAG 1 cut(s) 372
AcyI GRCGYC 2 cut(s) 8, 1132
AfiI CCNNNNNNNGG 4 cut(s) 55, 176, 485, 1329
AflIII ACRYGT 1 cut(s) 1473
AgeI ACCGGT 1 cut(s) 446
AgsI TTSAA 4 cut(s) 444, 603, 680, 1480
AhdI GACNNNNNGTC 1 cut(s) 919
AjnI CCWGG 3 cut(s) 666, 1344, 1405
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AluBI AGCT 8 cut(s) 308, 631, 638, 1046, 1072, 1358, 1513, 1656
AluI AGCT 8 cut(s) 308, 631, 638, 1046, 1072, 1358, 1513, 1656
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 3 cut(s) 277, 1015, 1526
AlwI GGATC 2 cut(s) 469, 862
ApaI GGGCCC 2 cut(s) 745, 1545
ApeKI GCWGC 2 cut(s) 1298, 1301
ApoI RAATTY 4 cut(s) 594, 662, 788, 1481
ArsI GACNNNNNNTTYG 2 cut(s) 145, 177
AseI ATTAAT 1 cut(s) 687
AsiGI ACCGGT 1 cut(s) 446
Asp700I GAANNNNTTC 2 cut(s) 408, 598
AspLEI GCGC 3 cut(s) 10, 1204, 1298
AspS9I GGNCC 6 cut(s) 13, 350, 741, 742, 1541, 1542
AsuHPI GGTGA 2 cut(s) 30, 1595
BaeGI GKGCMC 2 cut(s) 745, 1545
BaeI ACNNNNGTAYC 2 cut(s) 993, 1026
BalI TGGCCA 3 cut(s) 260, 829, 1560
BanI GGYRCC 1 cut(s) 7
BanII GRGCYC 2 cut(s) 745, 1545
BarI GAAGNNNNNNTAC 4 cut(s) 424, 456, 1386, 1418
BauI CACGAG 1 cut(s) 1455
BbsI GAAGAC 2 cut(s) 1084, 1476
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 2 cut(s) 1285, 1288
BccI CCATC 3 cut(s) 445, 705, 1223
BceAI ACGGC 2 cut(s) 172, 1311
BcgI CGANNNNNNTGC 4 cut(s) 50, 84, 187, 221
BciT130I CCWGG 3 cut(s) 668, 1346, 1407
BciVI GTATCC 2 cut(s) 719, 1031
BcoDI GTCTC 3 cut(s) 277, 1015, 1526
BfaI CTAG 3 cut(s) 1499, 1514, 1578
BfmI CTRYAG 1 cut(s) 849
BfoI RGCGCY 1 cut(s) 11
BfuI GTATCC 2 cut(s) 719, 1031
BglI GCCNNNNNGGC 1 cut(s) 986
BisI GCNGC 5 cut(s) 113, 137, 454, 1299, 1302
BlsI GCNGC 5 cut(s) 114, 138, 455, 1300, 1303
Bme1390I CCNGG 3 cut(s) 668, 1346, 1407
BmeRI GACNNNNNGTC 1 cut(s) 919
BmgT120I GGNCC 6 cut(s) 13, 350, 741, 742, 1541, 1542
BmiI GGNNCC 5 cut(s) 9, 15, 742, 743, 1543
BmrFI CCNGG 3 cut(s) 668, 1346, 1407
BmsI GCATC 2 cut(s) 158, 1067
BpiI GAAGAC 2 cut(s) 1084, 1476
BpmI CTGGAG 2 cut(s) 1252, 1362
BsaHI GRCGYC 2 cut(s) 8, 1132
BsaI GGTCTC 1 cut(s) 1526
BsaJI CCNNGG 1 cut(s) 783
BsaWI WCCGGW 1 cut(s) 446
BsaXI ACNNNNNCTCC 6 cut(s) 34, 64, 67, 70, 97, 100
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 176, 485, 1329
Bse118I RCCGGY 4 cut(s) 10, 55, 202, 446
Bse1I ACTGG 5 cut(s) 723, 1062, 1132, 1536, 1599
BseBI CCWGG 3 cut(s) 668, 1346, 1407
BseDI CCNNGG 1 cut(s) 783
BseGI GGATG 2 cut(s) 892, 1058
BseLI CCNNNNNNNGG 4 cut(s) 55, 176, 485, 1329
BseMII CTCAG 1 cut(s) 1087
BseNI ACTGG 5 cut(s) 723, 1062, 1132, 1536, 1599
BseRI GAGGAG 2 cut(s) 75, 372
BseSI GKGCMC 2 cut(s) 745, 1545
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 2 cut(s) 1285, 1288
BseYI CCCAGC 3 cut(s) 478, 638, 1544
Bsh1236I CGCG 3 cut(s) 107, 1202, 1204
Bsh1285I CGRYCG 2 cut(s) 60, 207
BshNI GGYRCC 1 cut(s) 7
BshTI ACCGGT 1 cut(s) 446
BsiEI CGRYCG 2 cut(s) 60, 207
BsiHKAI GWGCWC 1 cut(s) 200
BsiSI CCGG 5 cut(s) 11, 56, 146, 203, 447
BslI CCNNNNNNNGG 4 cut(s) 55, 176, 485, 1329
BsmAI GTCTC 3 cut(s) 277, 1015, 1526
BsmBI CGTCTC 1 cut(s) 277
Bso31I GGTCTC 1 cut(s) 1526
Bsp120I GGGCCC 2 cut(s) 741, 1541
Bsp1286I GDGCHC 3 cut(s) 200, 745, 1545
Bsp143I GATC 4 cut(s) 461, 854, 900, 1516
BspACI CCGC 7 cut(s) 53, 105, 113, 137, 453, 473, 1324
BspCNI CTCAG 1 cut(s) 1086
BspFNI CGCG 3 cut(s) 107, 1202, 1204
BspLI GGNNCC 5 cut(s) 9, 15, 742, 743, 1543
BspPI GGATC 2 cut(s) 469, 862
BspT107I GGYRCC 1 cut(s) 7
BspTNI GGTCTC 1 cut(s) 1526
BsrBI CCGCTC 1 cut(s) 475
BsrFI RCCGGY 4 cut(s) 10, 55, 202, 446
BsrI ACTGG 5 cut(s) 723, 1062, 1132, 1536, 1599
BssAI RCCGGY 4 cut(s) 10, 55, 202, 446
BssECI CCNNGG 1 cut(s) 783
BssMI GATC 4 cut(s) 461, 854, 900, 1516
BssNI GRCGYC 2 cut(s) 8, 1132
BssSI CACGAG 1 cut(s) 1455
BssT1I CCWWGG 1 cut(s) 783
Bst2BI CACGAG 1 cut(s) 1455
Bst2UI CCWGG 3 cut(s) 668, 1346, 1407
Bst4CI ACNGT 3 cut(s) 890, 911, 1427
BstACI GRCGYC 2 cut(s) 8, 1132
BstAPI GCANNNNNTGC 1 cut(s) 962
BstC8I GCNNGC 8 cut(s) 12, 57, 200, 1200, 1206, 1294, 1324, 1562
BstDEI CTNAG 3 cut(s) 991, 1042, 1073
BstF5I GGATG 2 cut(s) 892, 1058
BstFNI CGCG 3 cut(s) 107, 1202, 1204
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 3 cut(s) 10, 1204, 1298
BstKTI GATC 4 cut(s) 464, 857, 903, 1519
BstMAI GTCTC 3 cut(s) 277, 1015, 1526
BstMBI GATC 4 cut(s) 461, 854, 900, 1516
BstMCI CGRYCG 2 cut(s) 60, 207
BstMWI GCNNNNNNNGC 3 cut(s) 962, 986, 1298
BstNI CCWGG 3 cut(s) 668, 1346, 1407
BstNSI RCATGY 1 cut(s) 1477
BstSCI CCNGG 3 cut(s) 666, 1344, 1405
BstSFI CTRYAG 1 cut(s) 849
BstSLI GKGCMC 2 cut(s) 745, 1545
BstUI CGCG 3 cut(s) 107, 1202, 1204
BstV1I GCAGC 2 cut(s) 1285, 1288
BstV2I GAAGAC 2 cut(s) 1084, 1476
BstX2I RGATCY 1 cut(s) 854
BstXI CCANNNNNNTGG 2 cut(s) 1276, 1646
BstYI RGATCY 1 cut(s) 854
BstZI CGGCCG 1 cut(s) 57
BsuI GTATCC 2 cut(s) 719, 1031
BtgZI GCGATG 1 cut(s) 117
BtsCI GGATG 2 cut(s) 892, 1058
BtsI GCAGTG 1 cut(s) 1555
BtsIMutI CAGTG 7 cut(s) 247, 716, 1069, 1081, 1161, 1543, 1555
Cac8I GCNNGC 8 cut(s) 12, 57, 200, 1200, 1206, 1294, 1324, 1562
CfoI GCGC 3 cut(s) 10, 1204, 1298
Cfr10I RCCGGY 4 cut(s) 10, 55, 202, 446
Cfr13I GGNCC 6 cut(s) 13, 350, 741, 742, 1541, 1542
CseI GACGC 4 cut(s) 71, 96, 1140, 1301
CsiI ACCWGGT 1 cut(s) 1405
CspAI ACCGGT 1 cut(s) 446
CviAII CATG 6 cut(s) 526, 800, 881, 1007, 1174, 1474
DdeI CTNAG 3 cut(s) 991, 1042, 1073
DinI GGCGCC 1 cut(s) 9
DpnI GATC 4 cut(s) 463, 856, 902, 1518
DpnII GATC 4 cut(s) 461, 854, 900, 1516
DriI GACNNNNNGTC 1 cut(s) 919
EaeI YGGCCR 5 cut(s) 57, 258, 827, 1331, 1558
EagI CGGCCG 1 cut(s) 57
Eam1105I GACNNNNNGTC 1 cut(s) 919
EciI GGCGGA 1 cut(s) 42
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 783
Eco147I AGGCCT 1 cut(s) 1192
Eco24I GRGCYC 2 cut(s) 745, 1545
Eco31I GGTCTC 1 cut(s) 1526
Eco32I GATATC 1 cut(s) 1609
Eco52I CGGCCG 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 741
EcoRI GAATTC 2 cut(s) 594, 662
EcoRII CCWGG 3 cut(s) 666, 1344, 1405
EcoRV GATATC 1 cut(s) 1609
EcoT14I CCWWGG 1 cut(s) 783
EcoT22I ATGCAT 1 cut(s) 1060
EcoT38I GRGCYC 2 cut(s) 745, 1545
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 783
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 6 cut(s) 529, 803, 884, 1010, 1177, 1477
FalI AAGNNNNNCTT 8 cut(s) 582, 614, 974, 1006, 1208, 1240, 1356, 1388
FatI CATG 6 cut(s) 525, 799, 880, 1006, 1173, 1473
FauI CCCGC 1 cut(s) 1331
Fnu4HI GCNGC 5 cut(s) 113, 137, 454, 1299, 1302
FokI GGATG 2 cut(s) 879, 1045
FriOI GRGCYC 2 cut(s) 745, 1545
Fsp4HI GCNGC 5 cut(s) 113, 137, 454, 1299, 1302
FspBI CTAG 3 cut(s) 1499, 1514, 1578
GlaI GCGC 3 cut(s) 9, 1203, 1297
GluI GCNGC 5 cut(s) 113, 137, 454, 1299, 1302
GsaI CCCAGC 3 cut(s) 482, 642, 1548
GsuI CTGGAG 2 cut(s) 1252, 1362
HaeII RGCGCY 1 cut(s) 11
HapII CCGG 5 cut(s) 11, 56, 146, 203, 447
HgaI GACGC 4 cut(s) 71, 96, 1140, 1301
HhaI GCGC 3 cut(s) 10, 1204, 1298
Hin1I GRCGYC 2 cut(s) 8, 1132
Hin1II CATG 6 cut(s) 529, 803, 884, 1010, 1177, 1477
Hin6I GCGC 3 cut(s) 8, 1202, 1296
HinP1I GCGC 3 cut(s) 8, 1202, 1296
HincII GTYRAC 1 cut(s) 323
HindII GTYRAC 1 cut(s) 323
HinfI GANTC 1 cut(s) 314
HpaII CCGG 5 cut(s) 11, 56, 146, 203, 447
HphI GGTGA 2 cut(s) 30, 1595
Hpy166II GTNNAC 4 cut(s) 21, 323, 434, 647
Hpy188I TCNGA 7 cut(s) 90, 221, 282, 466, 496, 1087, 1435
Hpy188III TCNNGA 5 cut(s) 1049, 1379, 1457, 1604, 1659
Hpy8I GTNNAC 4 cut(s) 21, 323, 434, 647
Hpy99I CGWCG 4 cut(s) 65, 112, 193, 1332
HpyAV CCTTC 5 cut(s) 396, 1168, 1358, 1388, 1580
HpyCH4III ACNGT 3 cut(s) 890, 911, 1427
HpyCH4V TGCA 8 cut(s) 367, 620, 732, 799, 860, 965, 1058, 1252
HpyF10VI GCNNNNNNNGC 3 cut(s) 962, 986, 1298
HpyF3I CTNAG 3 cut(s) 991, 1042, 1073
Hsp92I GRCGYC 2 cut(s) 8, 1132
Hsp92II CATG 6 cut(s) 529, 803, 884, 1010, 1177, 1477
HspAI GCGC 3 cut(s) 8, 1202, 1296
KasI GGCGCC 1 cut(s) 7
KroI GCCGGC 2 cut(s) 10, 55
KroNI GCCGGC 2 cut(s) 12, 57
Kzo9I GATC 4 cut(s) 461, 854, 900, 1516
LmnI GCTCC 6 cut(s) 76, 195, 385, 480, 655, 1051
Lsp1109I GCAGC 2 cut(s) 1285, 1288
LweI GCATC 2 cut(s) 158, 1067
MabI ACCWGGT 1 cut(s) 1405
MaeI CTAG 3 cut(s) 1499, 1514, 1578
MaeIII GTNAC 1 cut(s) 911
MalI GATC 4 cut(s) 463, 856, 902, 1518
MbiI CCGCTC 1 cut(s) 475
MboI GATC 4 cut(s) 461, 854, 900, 1516
MboII GAAGA 6 cut(s) 259, 301, 432, 548, 1084, 1481
MflI RGATCY 1 cut(s) 854
MhlI GDGCHC 3 cut(s) 200, 745, 1545
MlsI TGGCCA 3 cut(s) 260, 829, 1560
MluNI TGGCCA 3 cut(s) 260, 829, 1560
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 5 cut(s) 113, 199, 570, 703, 798
Mox20I TGGCCA 3 cut(s) 260, 829, 1560
Mph1103I ATGCAT 1 cut(s) 1060
MroNI GCCGGC 2 cut(s) 10, 55
MroXI GAANNNNTTC 2 cut(s) 408, 598
MscI TGGCCA 3 cut(s) 260, 829, 1560
MseI TTAA 4 cut(s) 344, 624, 687, 1388
MslI CAYNNNNRTG 1 cut(s) 798
Msp20I TGGCCA 3 cut(s) 260, 829, 1560
MspA1I CMGCKG 1 cut(s) 631
MspI CCGG 5 cut(s) 11, 56, 146, 203, 447
MspR9I CCNGG 3 cut(s) 668, 1346, 1407
MvaI CCWGG 3 cut(s) 668, 1346, 1407
MvnI CGCG 3 cut(s) 107, 1202, 1204
MwoI GCNNNNNNNGC 3 cut(s) 962, 986, 1298
NaeI GCCGGC 2 cut(s) 12, 57
NarI GGCGCC 1 cut(s) 8
NdeII GATC 4 cut(s) 461, 854, 900, 1516
NgoMIV GCCGGC 2 cut(s) 10, 55
NlaIII CATG 6 cut(s) 529, 803, 884, 1010, 1177, 1477
NlaIV GGNNCC 5 cut(s) 9, 15, 742, 743, 1543
NmuCI GTSAC 1 cut(s) 911
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 1 cut(s) 1477
PceI AGGCCT 1 cut(s) 1192
PciI ACATGT 1 cut(s) 1473
PdiI GCCGGC 2 cut(s) 12, 57
PdmI GAANNNNTTC 2 cut(s) 408, 598
PflMI CCANNNNNTGG 1 cut(s) 485
PinAI ACCGGT 1 cut(s) 446
PkrI GCNGC 5 cut(s) 114, 138, 455, 1300, 1303
PleI GAGTC 1 cut(s) 322
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 322
PscI ACATGT 1 cut(s) 1473
PshBI ATTAAT 1 cut(s) 687
Psp6I CCWGG 3 cut(s) 666, 1344, 1405
PspFI CCCAGC 3 cut(s) 478, 638, 1544
PspGI CCWGG 3 cut(s) 666, 1344, 1405
PspN4I GGNNCC 5 cut(s) 9, 15, 742, 743, 1543
PspOMI GGGCCC 2 cut(s) 741, 1541
PspPI GGNCC 6 cut(s) 13, 350, 741, 742, 1541, 1542
PsuI RGATCY 1 cut(s) 854
PvuII CAGCTG 1 cut(s) 631
RseI CAYNNNNRTG 1 cut(s) 798
SaqAI TTAA 4 cut(s) 344, 624, 687, 1388
SatI GCNGC 5 cut(s) 113, 137, 454, 1299, 1302
Sau3AI GATC 4 cut(s) 461, 854, 900, 1516
Sau96I GGNCC 6 cut(s) 13, 350, 741, 742, 1541, 1542
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 3 cut(s) 668, 1346, 1407
SduI GDGCHC 3 cut(s) 200, 745, 1545
SexAI ACCWGGT 1 cut(s) 1405
SfaNI GCATC 2 cut(s) 158, 1067
SfcI CTRYAG 1 cut(s) 849
SfoI GGCGCC 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 798
SseBI AGGCCT 1 cut(s) 1192
SsiI CCGC 7 cut(s) 53, 105, 113, 137, 453, 473, 1324
SspDI GGCGCC 1 cut(s) 7
SspI AATATT 1 cut(s) 931
SspMI CTAG 3 cut(s) 1499, 1514, 1578
StuI AGGCCT 1 cut(s) 1192
StyD4I CCNGG 3 cut(s) 666, 1344, 1405
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 3 cut(s) 890, 911, 1427
TaqI TCGA 3 cut(s) 152, 1354, 1611
TauI GCSGC 3 cut(s) 115, 139, 456
Tru1I TTAA 4 cut(s) 344, 624, 687, 1388
Tru9I TTAA 4 cut(s) 344, 624, 687, 1388
TscAI CASTG 7 cut(s) 247, 723, 1069, 1081, 1168, 1543, 1555
TseFI GTSAC 1 cut(s) 911
TseI GCWGC 2 cut(s) 1298, 1301
Tsp45I GTSAC 1 cut(s) 911
TspDTI ATGAA 5 cut(s) 854, 1257, 1398, 1407, 1640
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 7 cut(s) 247, 723, 1069, 1081, 1168, 1543, 1555
Van91I CCANNNNNTGG 1 cut(s) 485
VspI ATTAAT 1 cut(s) 687
XapI RAATTY 4 cut(s) 594, 662, 788, 1481
XceI RCATGY 1 cut(s) 1477
XcmI CCANNNNNNNNNTGG 3 cut(s) 781, 822, 1021
XmnI GAANNNNTTC 2 cut(s) 408, 598
XspI CTAG 3 cut(s) 1499, 1514, 1578
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.