Rorug02G0399300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000193
Physical Location & Seq
Forward (+)
51049039 .. 51053309
4271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0399300.1

Sequence Viewer

Length: 624 bp
ATGGCTTCTCGCAGACGCATGCTTCTAAAGGTCATTATCCTCGGCGACAGCGGGGTGGGGAAGACATCGCTTATGAATCAGTATGTGAATCGTAAGTTTAGTAATCAGTACAAGGCGACAATCGGAGCGGATTTTCTGACCAAGGAGGTTCAGTTTGAAGATAGGTTGTTCACATTGCAGATTTGGGATACTGCTGGGCAAGAAAGGTTTCAAAGTCTTGGTGTGGCTTTCTATCGTGGTGCAGATTGTTGTGTGCTTGTGTATGATGTGAATGTCATGAAATCATTTGATAATCTTAACAACTGGAGAGAAGAATTTCTGATTCAGGCTAGTCCATCCGATCCCGAGAACTTTCCATTTGTTGTGTTGGGGAACAAGATTGATGTTGATGGTGGAAATAGTCGAGTGGTTTCTGAAAAGAAAGCGAAGGCCTGGTGTGCTTCCAAGGGAAACATACCATACTTTGAGACCTCTGCAAAAGAAGGCTTTAACGTGGATGATGCCTTCCAATGTATAGCGAAAAACGCACTGAAGAATGAACCTGAAGAAGAAATATACCTTCCTGACACAATCGATGTTGCCGGTGGAGGGAGGCAGCAAAGATCTACTGGCTGTGAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

207

Amino Acids

23.23

Weight (kDa)

5.21

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 7 - 174 5.6e-13 ADP-ribosylation factor family
Ras PF00071 10 - 176 7.8e-55 Ras family
Roc PF08477 10 - 128 8.3e-33 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 10 - 105 4.1e-07 50S ribosome-binding GTPase
GTP_EFTU PF00009 47 - 174 2.1e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 128
AciI CCGC 2 cut(s) 51, 128
AclWI GGATC 1 cut(s) 335
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 2 cut(s) 551, 564
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 1 cut(s) 588
AgsI TTSAA 2 cut(s) 158, 212
AjnI CCWGG 1 cut(s) 431
Alw26I GTCTC 1 cut(s) 461
AlwI GGATC 1 cut(s) 335
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 1 cut(s) 429
ApeKI GCWGC 1 cut(s) 595
ApoI RAATTY 1 cut(s) 314
Asp700I GAANNNNTTC 2 cut(s) 207, 315
AvaI CYCGRG 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 1 cut(s) 607
BccI CCATC 2 cut(s) 343, 383
BciT130I CCWGG 1 cut(s) 433
BciVI GTATCC 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 1 cut(s) 330
BfuI GTATCC 1 cut(s) 181
BglII AGATCT 1 cut(s) 602
BisI GCNGC 1 cut(s) 596
BlsI GCNGC 1 cut(s) 597
Bme1390I CCNGG 1 cut(s) 433
BmeT110I CYCGRG 1 cut(s) 344
BmrFI CCNGG 1 cut(s) 433
BmsI GCATC 1 cut(s) 490
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 325
Bsa29I ATCGAT 1 cut(s) 573
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 40, 141, 444
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 588
Bse118I RCCGGY 1 cut(s) 581
Bse1I ACTGG 2 cut(s) 308, 613
Bse3DI GCAATG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 433
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 3 cut(s) 40, 141, 444
BseGI GGATG 2 cut(s) 335, 502
BseLI CCNNNNNNNGG 1 cut(s) 588
BseMI GCAATG 1 cut(s) 173
BseNI ACTGG 2 cut(s) 308, 613
BseXI GCAGC 1 cut(s) 607
BseYI CCCAGC 1 cut(s) 194
BsgI GTGCAG 1 cut(s) 261
BshFI GGCC 1 cut(s) 431
BshVI ATCGAT 1 cut(s) 573
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 1 cut(s) 582
BslI CCNNNNNNNGG 1 cut(s) 588
BsmAI GTCTC 1 cut(s) 461
BsnI GGCC 1 cut(s) 431
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 344
Bsp143I GATC 2 cut(s) 340, 602
BspACI CCGC 2 cut(s) 51, 128
BspANI GGCC 1 cut(s) 431
BspDI ATCGAT 1 cut(s) 573
BspHI TCATGA 1 cut(s) 276
BspPI GGATC 1 cut(s) 335
BspTNI GGTCTC 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 581
BsrI ACTGG 2 cut(s) 308, 613
BssAI RCCGGY 1 cut(s) 581
BssECI CCNNGG 3 cut(s) 40, 141, 444
BssMI GATC 2 cut(s) 340, 602
BssT1I CCWWGG 2 cut(s) 141, 444
Bst2UI CCWGG 1 cut(s) 433
BstC8I GCNNGC 1 cut(s) 20
BstF5I GGATG 2 cut(s) 335, 502
BstKTI GATC 2 cut(s) 343, 605
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 2 cut(s) 340, 602
BstMWI GCNNNNNNNGC 2 cut(s) 437, 524
BstNI CCWGG 1 cut(s) 433
BstNSI RCATGY 1 cut(s) 22
BstSCI CCNGG 1 cut(s) 431
BstV1I GCAGC 1 cut(s) 607
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 602
BstYI RGATCY 1 cut(s) 602
Bsu15I ATCGAT 1 cut(s) 573
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 1 cut(s) 431
BsuTUI ATCGAT 1 cut(s) 573
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 335, 502
BtsIMutI CAGTG 1 cut(s) 527
Cac8I GCNNGC 1 cut(s) 20
CciI TCATGA 1 cut(s) 276
Cfr10I RCCGGY 1 cut(s) 581
ClaI ATCGAT 1 cut(s) 573
CseI GACGC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 109
CviAII CATG 2 cut(s) 19, 277
CviJI RGCY 6 cut(s) 5, 227, 329, 431, 486, 612
CviKI_1 RGCY 6 cut(s) 5, 227, 329, 431, 486, 612
CviQI GTAC 1 cut(s) 109
DpnI GATC 2 cut(s) 342, 604
DpnII GATC 2 cut(s) 340, 602
Eco130I CCWWGG 2 cut(s) 141, 444
Eco147I AGGCCT 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 461
Eco57I CTGAAG 2 cut(s) 551, 564
Eco88I CYCGRG 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 431
EcoT14I CCWWGG 2 cut(s) 141, 444
ErhI CCWWGG 2 cut(s) 141, 444
FaeI CATG 2 cut(s) 22, 280
FaiI YATR 9 cut(s) 20, 74, 84, 264, 278, 455, 460, 515, 556
FatI CATG 2 cut(s) 18, 276
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 596
FokI GGATG 2 cut(s) 322, 509
Fsp4HI GCNGC 1 cut(s) 596
FspBI CTAG 1 cut(s) 330
GluI GCNGC 1 cut(s) 596
GsaI CCCAGC 1 cut(s) 198
GsuI CTGGAG 1 cut(s) 325
HaeIII GGCC 1 cut(s) 431
HapII CCGG 1 cut(s) 582
HgaI GACGC 1 cut(s) 24
Hin1II CATG 2 cut(s) 22, 280
HinfI GANTC 3 cut(s) 76, 88, 322
HpaII CCGG 1 cut(s) 582
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 5 cut(s) 125, 138, 321, 340, 415
Hpy188III TCNNGA 3 cut(s) 277, 344, 563
Hpy8I GTNNAC 1 cut(s) 171
HpyAV CCTTC 4 cut(s) 421, 476, 514, 569
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 3 cut(s) 178, 242, 476
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 524
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 2 cut(s) 22, 280
Kzo9I GATC 2 cut(s) 340, 602
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 9 cut(s) 180, 289, 311, 418, 445, 555, 576, 594, 595
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 1 cut(s) 490
MaeI CTAG 1 cut(s) 330
MaeII ACGT 1 cut(s) 492
MalI GATC 2 cut(s) 342, 604
MbiI CCGCTC 1 cut(s) 128
MboI GATC 2 cut(s) 340, 602
MboII GAAGA 6 cut(s) 73, 170, 323, 544, 557, 560
MflI RGATCY 1 cut(s) 602
MluCI AATT 1 cut(s) 314
MnlI CCTC 5 cut(s) 50, 139, 481, 581, 585
MroXI GAANNNNTTC 2 cut(s) 207, 315
MseI TTAA 2 cut(s) 297, 489
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 1 cut(s) 582
MspR9I CCNGG 1 cut(s) 433
MvaI CCWGG 1 cut(s) 433
MwoI GCNNNNNNNGC 2 cut(s) 437, 524
NdeII GATC 2 cut(s) 340, 602
NlaIII CATG 2 cut(s) 22, 280
NmeAIII GCCGAG 1 cut(s) 21
NspI RCATGY 1 cut(s) 22
PaeI GCATGC 1 cut(s) 22
PagI TCATGA 1 cut(s) 276
PceI AGGCCT 1 cut(s) 431
PdmI GAANNNNTTC 2 cut(s) 207, 315
PfeI GAWTC 3 cut(s) 76, 88, 322
PkrI GCNGC 1 cut(s) 597
Psp6I CCWGG 1 cut(s) 431
PspFI CCCAGC 1 cut(s) 194
PspGI CCWGG 1 cut(s) 431
PsuI RGATCY 1 cut(s) 602
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SaqAI TTAA 2 cut(s) 297, 489
SatI GCNGC 1 cut(s) 596
Sau3AI GATC 2 cut(s) 340, 602
ScrFI CCNGG 1 cut(s) 433
SetI ASST 8 cut(s) 33, 150, 167, 209, 473, 495, 544, 561
SfaNI GCATC 1 cut(s) 490
SphI GCATGC 1 cut(s) 22
Sse9I AATT 1 cut(s) 314
SseBI AGGCCT 1 cut(s) 431
SsiI CCGC 2 cut(s) 51, 128
SspMI CTAG 1 cut(s) 330
StuI AGGCCT 1 cut(s) 431
StyD4I CCNGG 1 cut(s) 431
StyI CCWWGG 2 cut(s) 141, 444
TaiI ACGT 1 cut(s) 495
TaqI TCGA 2 cut(s) 403, 573
TasI AATT 1 cut(s) 314
TatI WGTACW 1 cut(s) 108
TfiI GAWTC 3 cut(s) 76, 88, 322
Tru1I TTAA 2 cut(s) 297, 489
Tru9I TTAA 2 cut(s) 297, 489
TscAI CASTG 1 cut(s) 534
TseI GCWGC 1 cut(s) 595
TspDTI ATGAA 3 cut(s) 89, 293, 552
TspRI CASTG 1 cut(s) 534
XapI RAATTY 1 cut(s) 314
XceI RCATGY 1 cut(s) 22
XmnI GAANNNNTTC 2 cut(s) 207, 315
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.