Belongs to the 'GDSL' lipolytic enzyme family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 218 |
| AciI | CCGC | 3 cut(s) | 57, 93, 245 |
| AclWI | GGATC | 1 cut(s) | 235 |
| AcoI | YGGCCR | 2 cut(s) | 143, 151 |
| AcsI | RAATTY | 1 cut(s) | 167 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 266 |
| AgsI | TTSAA | 1 cut(s) | 166 |
| Alw26I | GTCTC | 1 cut(s) | 190 |
| AlwI | GGATC | 1 cut(s) | 235 |
| AoxI | GGCC | 2 cut(s) | 143, 151 |
| ApoI | RAATTY | 1 cut(s) | 167 |
| BalI | TGGCCA | 1 cut(s) | 145 |
| BbsI | GAAGAC | 1 cut(s) | 12 |
| BccI | CCATC | 1 cut(s) | 83 |
| BceAI | ACGGC | 1 cut(s) | 166 |
| BcoDI | GTCTC | 1 cut(s) | 190 |
| BmrI | ACTGGG | 1 cut(s) | 117 |
| BmuI | ACTGGG | 1 cut(s) | 117 |
| BpiI | GAAGAC | 1 cut(s) | 12 |
| Bsa29I | ATCGAT | 1 cut(s) | 111 |
| BsaWI | WCCGGW | 1 cut(s) | 176 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 66, 96 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 266 |
| Bse1I | ACTGG | 3 cut(s) | 123, 146, 248 |
| BseCI | ATCGAT | 1 cut(s) | 111 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 266 |
| BseNI | ACTGG | 3 cut(s) | 123, 146, 248 |
| Bsh1236I | CGCG | 1 cut(s) | 218 |
| BshFI | GGCC | 2 cut(s) | 145, 153 |
| BshVI | ATCGAT | 1 cut(s) | 111 |
| BsiSI | CCGG | 1 cut(s) | 177 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 266 |
| BsmAI | GTCTC | 1 cut(s) | 190 |
| BsnI | GGCC | 2 cut(s) | 145, 153 |
| Bsp143I | GATC | 2 cut(s) | 131, 240 |
| Bsp68I | TCGCGA | 1 cut(s) | 218 |
| BspACI | CCGC | 3 cut(s) | 57, 93, 245 |
| BspANI | GGCC | 2 cut(s) | 145, 153 |
| BspDI | ATCGAT | 1 cut(s) | 111 |
| BspFNI | CGCG | 1 cut(s) | 218 |
| BspPI | GGATC | 1 cut(s) | 235 |
| BsrI | ACTGG | 3 cut(s) | 123, 146, 248 |
| BssMI | GATC | 2 cut(s) | 131, 240 |
| Bst4CI | ACNGT | 2 cut(s) | 237, 269 |
| Bst6I | CTCTTC | 2 cut(s) | 123, 175 |
| BstEII | GGTNACC | 1 cut(s) | 231 |
| BstFNI | CGCG | 1 cut(s) | 218 |
| BstKTI | GATC | 2 cut(s) | 134, 243 |
| BstMAI | GTCTC | 1 cut(s) | 190 |
| BstMBI | GATC | 2 cut(s) | 131, 240 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 224 |
| BstPI | GGTNACC | 1 cut(s) | 231 |
| BstUI | CGCG | 1 cut(s) | 218 |
| BstV2I | GAAGAC | 1 cut(s) | 12 |
| Bsu15I | ATCGAT | 1 cut(s) | 111 |
| BsuRI | GGCC | 2 cut(s) | 145, 153 |
| BsuTUI | ATCGAT | 1 cut(s) | 111 |
| BtgZI | GCGATG | 1 cut(s) | 199 |
| BtuMI | TCGCGA | 1 cut(s) | 218 |
| ClaI | ATCGAT | 1 cut(s) | 111 |
| CseI | GACGC | 1 cut(s) | 204 |
| CviAII | CATG | 1 cut(s) | 228 |
| CviJI | RGCY | 2 cut(s) | 145, 153 |
| CviKI_1 | RGCY | 2 cut(s) | 145, 153 |
| DpnI | GATC | 2 cut(s) | 133, 242 |
| DpnII | GATC | 2 cut(s) | 131, 240 |
| EaeI | YGGCCR | 2 cut(s) | 143, 151 |
| Eam1104I | CTCTTC | 2 cut(s) | 123, 175 |
| EarI | CTCTTC | 2 cut(s) | 123, 175 |
| EciI | GGCGGA | 1 cut(s) | 108 |
| Eco91I | GGTNACC | 1 cut(s) | 231 |
| EcoO65I | GGTNACC | 1 cut(s) | 231 |
| EcoT22I | ATGCAT | 1 cut(s) | 229 |
| FaeI | CATG | 1 cut(s) | 231 |
| FaiI | YATR | 6 cut(s) | 148, 225, 229, 279, 281, 287 |
| FatI | CATG | 1 cut(s) | 227 |
| FauI | CCCGC | 1 cut(s) | 252 |
| HaeIII | GGCC | 2 cut(s) | 145, 153 |
| HapII | CCGG | 1 cut(s) | 177 |
| HgaI | GACGC | 1 cut(s) | 204 |
| Hin1II | CATG | 1 cut(s) | 231 |
| HinfI | GANTC | 2 cut(s) | 77, 183 |
| HpaII | CCGG | 1 cut(s) | 177 |
| Hpy188I | TCNGA | 1 cut(s) | 190 |
| Hpy188III | TCNNGA | 2 cut(s) | 206, 217 |
| Hpy99I | CGWCG | 1 cut(s) | 194 |
| HpyCH4III | ACNGT | 2 cut(s) | 237, 269 |
| HpyCH4V | TGCA | 1 cut(s) | 227 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 224 |
| Hsp92II | CATG | 1 cut(s) | 231 |
| Kzo9I | GATC | 2 cut(s) | 131, 240 |
| LpnPI | CCDG | 5 cut(s) | 26, 127, 136, 190, 261 |
| MaeIII | GTNAC | 4 cut(s) | 43, 231, 263, 269 |
| MalI | GATC | 2 cut(s) | 133, 242 |
| MboI | GATC | 2 cut(s) | 131, 240 |
| MboII | GAAGA | 3 cut(s) | 17, 110, 192 |
| MlsI | TGGCCA | 1 cut(s) | 145 |
| MluCI | AATT | 3 cut(s) | 136, 167, 201 |
| MluNI | TGGCCA | 1 cut(s) | 145 |
| MlyI | GAGTC | 1 cut(s) | 192 |
| MmeI | TCCRAC | 1 cut(s) | 213 |
| MnlI | CCTC | 1 cut(s) | 282 |
| Mox20I | TGGCCA | 1 cut(s) | 145 |
| Mph1103I | ATGCAT | 1 cut(s) | 229 |
| MscI | TGGCCA | 1 cut(s) | 145 |
| Msp20I | TGGCCA | 1 cut(s) | 145 |
| MspI | CCGG | 1 cut(s) | 177 |
| MvnI | CGCG | 1 cut(s) | 218 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 224 |
| NdeII | GATC | 2 cut(s) | 131, 240 |
| NlaIII | CATG | 1 cut(s) | 231 |
| NruI | TCGCGA | 1 cut(s) | 218 |
| NsiI | ATGCAT | 1 cut(s) | 229 |
| PfeI | GAWTC | 1 cut(s) | 77 |
| PleI | GAGTC | 1 cut(s) | 191 |
| PpsI | GAGTC | 1 cut(s) | 191 |
| PspEI | GGTNACC | 1 cut(s) | 231 |
| RruI | TCGCGA | 1 cut(s) | 218 |
| Sau3AI | GATC | 2 cut(s) | 131, 240 |
| SchI | GAGTC | 1 cut(s) | 192 |
| SetI | ASST | 1 cut(s) | 293 |
| Sse9I | AATT | 3 cut(s) | 136, 167, 201 |
| SsiI | CCGC | 3 cut(s) | 57, 93, 245 |
| TaaI | ACNGT | 2 cut(s) | 237, 269 |
| TaqI | TCGA | 2 cut(s) | 111, 130 |
| TasI | AATT | 3 cut(s) | 136, 167, 201 |
| TfiI | GAWTC | 1 cut(s) | 77 |
| TspDTI | ATGAA | 1 cut(s) | 202 |
| TspGWI | ACGGA | 1 cut(s) | 207 |
| XapI | RAATTY | 1 cut(s) | 167 |
| Zsp2I | ATGCAT | 1 cut(s) | 229 |