RLG00000020247

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000193
Physical Location & Seq
Reverse (-)
62821790 .. 62823972
2183 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020247

Sequence Viewer

Length: 810 bp
ATGGCTTCTTATCCTACCGGCTTCATATTTCTTCCTGCACAAGGACAAAGCGCCACCCGCTTTCGAGATGTCATGGCATTGATTCTTTTGCCAATTGTGGCCATAGGCTTAAACACTCAATTGAGTTATTTCAAGAATGTGGAGAAGCAATTGAGGCACAAACTAGGTGACGGAGAAGCCTACACAGTTCTTTCTAAAGCTGTTTACCTAATTAGCATTGGAACCAATGATTATTATACCCCTCTAATGAAAAATTACAGCTTGACTCAAGATGAAGAATATGTTGGCATGGTGATTGGCAACCTCACAGATGTGATCAGAGAAATATATAAGGTAGGTGGAAGGAAATTCGGGTTTGCAAACGTGTTACCTCTGGGCTGTTTACCAAGCTTTAGAATAAAGAATCCAGAAAACATTGGAGCCTGTATGAAAGAAGCAATGTCACTGGTGAAATCTCACAATAAAGAACTTGCTAAAGTGCTCGTAAAGTTGAAGAGCCAGCTCCAAGGATTCAAATATTCCAATGCCGATTTCTTCGCATACCTAACTGAAAGAATGAATAACCCATCAAAATACGGTTTCAGGGAAGGAAAAGCGGCATGTTGTGGCTCGGGTCCATACAGAGGACATCAGAACTGTGGAGGGAAGGGAGGTGTGAAAGAGTATGAATTATGTGACAATGTTACCGAATATGTTTTTTTTGACAATCATCCTACTGAAAGGGTTTACCATCAAGTTTCCAAGATATGGTGGAACCAAACTCCAAATGTCACAGGGCTTTACAATCTGAGATCTCTATTTGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

270

Amino Acids

30.34

Weight (kDa)

8.97

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 248 1.6e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 747
AciI CCGC 2 cut(s) 58, 596
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 1 cut(s) 347
AfiI CCNNNNNNNGG 3 cut(s) 41, 623, 747
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 4 cut(s) 133, 493, 514, 803
AjuI GAANNNNNNNTTGG 4 cut(s) 267, 299, 515, 547
AleI CACNNNNGTG 1 cut(s) 311
AluBI AGCT 4 cut(s) 200, 261, 390, 502
AluI AGCT 4 cut(s) 200, 261, 390, 502
Alw21I GWGCWC 1 cut(s) 483
Ama87I CYCGRG 1 cut(s) 610
AoxI GGCC 1 cut(s) 99
ApoI RAATTY 1 cut(s) 347
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 614
AsuHPI GGTGA 3 cut(s) 179, 304, 460
AvaI CYCGRG 1 cut(s) 610
AvaII GGWCC 1 cut(s) 614
BalI TGGCCA 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 483
BccI CCATC 2 cut(s) 574, 738
BclI TGATCA 1 cut(s) 315
BfaI CTAG 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 54
BglII AGATCT 1 cut(s) 791
BisI GCNGC 1 cut(s) 597
BlsI GCNGC 1 cut(s) 598
Bme18I GGWCC 1 cut(s) 614
BmeT110I CYCGRG 1 cut(s) 610
BmgT120I GGNCC 1 cut(s) 614
BmiI GGNNCC 4 cut(s) 223, 421, 615, 755
BpuEI CTTGAG 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 505
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 623, 747
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 450
Bse3DI GCAATG 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 505
BseGI GGATG 1 cut(s) 709
BseLI CCNNNNNNNGG 3 cut(s) 41, 623, 747
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 1 cut(s) 779
BseNI ACTGG 1 cut(s) 450
BsgI GTGCAG 1 cut(s) 21
BshFI GGCC 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 483
BsiHKCI CYCGRG 1 cut(s) 610
BsiSI CCGG 1 cut(s) 18
BslI CCNNNNNNNGG 3 cut(s) 41, 623, 747
BsnI GGCC 1 cut(s) 101
BsoBI CYCGRG 1 cut(s) 610
Bsp1286I GDGCHC 1 cut(s) 483
Bsp143I GATC 2 cut(s) 315, 791
BspACI CCGC 2 cut(s) 58, 596
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 1 cut(s) 780
BspLI GGNNCC 4 cut(s) 223, 421, 615, 755
BspQI GCTCTTC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 17
BsrI ACTGG 1 cut(s) 450
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 505
BssMI GATC 2 cut(s) 315, 791
BssT1I CCWWGG 1 cut(s) 505
Bst4CI ACNGT 3 cut(s) 187, 578, 638
Bst6I CTCTTC 1 cut(s) 488
BstC8I GCNNGC 1 cut(s) 500
BstDEI CTNAG 1 cut(s) 788
BstENI CCTNNNNNAGG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 709
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 2 cut(s) 318, 794
BstMBI GATC 2 cut(s) 315, 791
BstMWI GCNNNNNNNGC 2 cut(s) 57, 154
BstNSI RCATGY 1 cut(s) 603
BstX2I RGATCY 1 cut(s) 791
BstYI RGATCY 1 cut(s) 791
BsuRI GGCC 1 cut(s) 101
BtsCI GGATG 1 cut(s) 709
BtsIMutI CAGTG 1 cut(s) 443
Cac8I GCNNGC 1 cut(s) 500
CfoI GCGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 1 cut(s) 614
CviAII CATG 3 cut(s) 73, 289, 600
DdeI CTNAG 1 cut(s) 788
DpnI GATC 2 cut(s) 317, 793
DpnII GATC 2 cut(s) 315, 791
EaeI YGGCCR 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 488
Eco130I CCWWGG 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 614
Eco88I CYCGRG 1 cut(s) 610
EcoNI CCTNNNNNAGG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 505
ErhI CCWWGG 1 cut(s) 505
FaeI CATG 3 cut(s) 76, 292, 603
FatI CATG 3 cut(s) 72, 288, 599
FauI CCCGC 1 cut(s) 65
FbaI TGATCA 1 cut(s) 315
Fnu4HI GCNGC 1 cut(s) 597
FokI GGATG 1 cut(s) 696
Fsp4HI GCNGC 1 cut(s) 597
FspBI CTAG 1 cut(s) 164
GlaI GCGC 1 cut(s) 52
GluI GCNGC 1 cut(s) 597
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 18
HhaI GCGC 1 cut(s) 53
Hin1II CATG 3 cut(s) 76, 292, 603
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 388
HinfI GANTC 4 cut(s) 82, 265, 403, 510
HpaII CCGG 1 cut(s) 18
HphI GGTGA 3 cut(s) 179, 304, 460
Hpy166II GTNNAC 3 cut(s) 205, 383, 727
Hpy188I TCNGA 3 cut(s) 320, 633, 789
Hpy188III TCNNGA 4 cut(s) 65, 133, 269, 407
Hpy8I GTNNAC 3 cut(s) 205, 383, 727
HpyAV CCTTC 3 cut(s) 336, 581, 640
HpyCH4III ACNGT 3 cut(s) 187, 578, 638
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 2 cut(s) 38, 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 154
HpyF3I CTNAG 1 cut(s) 788
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 3 cut(s) 76, 292, 603
HspAI GCGC 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 315
Kzo9I GATC 2 cut(s) 315, 791
LguI GCTCTTC 1 cut(s) 488
LmnI GCTCC 2 cut(s) 419, 507
LpnPI CCDG 9 cut(s) 31, 48, 359, 420, 431, 436, 512, 568, 759
MaeI CTAG 1 cut(s) 164
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 6 cut(s) 167, 366, 441, 674, 682, 769
MalI GATC 2 cut(s) 317, 793
MboI GATC 2 cut(s) 315, 791
MboII GAAGA 4 cut(s) 23, 287, 505, 526
MfeI CAATTG 3 cut(s) 93, 119, 149
MflI RGATCY 1 cut(s) 791
MhlI GDGCHC 1 cut(s) 483
MlsI TGGCCA 1 cut(s) 101
MluCI AATT 7 cut(s) 93, 119, 149, 210, 253, 347, 668
MluNI TGGCCA 1 cut(s) 101
MlyI GAGTC 1 cut(s) 259
MnlI CCTC 7 cut(s) 147, 252, 314, 381, 617, 635, 644
Mox20I TGGCCA 1 cut(s) 101
MscI TGGCCA 1 cut(s) 101
MseI TTAA 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 311
Msp20I TGGCCA 1 cut(s) 101
MspI CCGG 1 cut(s) 18
MunI CAATTG 3 cut(s) 93, 119, 149
MwoI GCNNNNNNNGC 2 cut(s) 57, 154
NdeII GATC 2 cut(s) 315, 791
NlaIII CATG 3 cut(s) 76, 292, 603
NlaIV GGNNCC 4 cut(s) 223, 421, 615, 755
NmuCI GTSAC 4 cut(s) 167, 441, 674, 769
NspI RCATGY 1 cut(s) 603
OliI CACNNNNGTG 1 cut(s) 311
PciSI GCTCTTC 1 cut(s) 488
PfeI GAWTC 3 cut(s) 82, 403, 510
PflMI CCANNNNNTGG 1 cut(s) 747
PkrI GCNGC 1 cut(s) 598
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PspN4I GGNNCC 4 cut(s) 223, 421, 615, 755
PspPI GGNCC 1 cut(s) 614
PsuI RGATCY 1 cut(s) 791
RseI CAYNNNNRTG 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 488
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 1 cut(s) 597
Sau3AI GATC 2 cut(s) 315, 791
Sau96I GGNCC 1 cut(s) 614
SchI GAGTC 1 cut(s) 259
SduI GDGCHC 1 cut(s) 483
SinI GGWCC 1 cut(s) 614
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
Sse9I AATT 7 cut(s) 93, 119, 149, 210, 253, 347, 668
SsiI CCGC 2 cut(s) 58, 596
SspI AATATT 1 cut(s) 518
SspMI CTAG 1 cut(s) 164
StyI CCWWGG 1 cut(s) 505
TaaI ACNGT 3 cut(s) 187, 578, 638
TaiI ACGT 1 cut(s) 366
TaqI TCGA 1 cut(s) 64
TasI AATT 7 cut(s) 93, 119, 149, 210, 253, 347, 668
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 3 cut(s) 82, 403, 510
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TscAI CASTG 1 cut(s) 450
TseFI GTSAC 4 cut(s) 167, 441, 674, 769
Tsp45I GTSAC 4 cut(s) 167, 441, 674, 769
TspDTI ATGAA 6 cut(s) 13, 263, 288, 443, 572, 681
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 1 cut(s) 450
Van91I CCANNNNNTGG 1 cut(s) 747
VpaK11BI GGWCC 1 cut(s) 614
XagI CCTNNNNNAGG 1 cut(s) 39
XapI RAATTY 1 cut(s) 347
XceI RCATGY 1 cut(s) 603
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.