Rh2AG456000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000193
Physical Location & Seq
Reverse (-)
67035274 .. 67036831
1558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG456000.1

Sequence Viewer

Length: 1098 bp
ATGGCAACTTTCAAGTTTCCTATCTACATCCTGGCTTTATGTGCAGGCTTTCTTATTCCAAGCAGCTGTCATGATGGTTATACTGGGATTCAGAAAAAACATGCAGCCTTGTTCATCTTTGGTGATTCAGTATTTGATGTTGGAAACAATAACTACATAAATACAACCACTACTTTTCAGTCAAATTTCTGGCCCTATGGGGAATCTTATTTCAGATACCCAACTGGCAGATTTTCTGATGGGCGTCTAATCCCGGATTTTATAGCTGAGTATGCAAATTTACCTTTTATACAACCATACTTACAACCTGGGTTCGATGACTATACTTATGGGGTGAACTTTGCTTCTGGTGGGGGTGGTGCTCTAGCTGAAACATATCAAGCATTTGCCATAGGCTTAAACACTCAATTGAGTTATTTCAAGAATGTGGAGAAGCAATTGAGCCACAAACTAGGTGATGGAGAAGCCTACACATTTCTTTCTAAAGCTGTTTACCTAATTAGCATTGGAACCAATGATTATTATACCCCTCTTATGAAAAATTACAGCTCAACTCAAGATGAAGAATATGTTGGCATGGTGATTGGCAACCTCACAGATGTGATCAGAGAAATATATAAGGTAGGTGGAAGGAAATTCGGGTTTGCAAACGTGTTACCTCTGGGTTGTTTACCAAGCTTTAGAATAAAGAATCCAGAAAACAGTGGAGCCTGTATGAAAGAAGCTATGTCACTGGTGAGATCTCACAATAAAGAACTTGCTAAAGTGCTCCTAAAGTTGAAGAGCCAGCTCCAAGGATTCAAATATTCCAATGCCGATTTCTACACATACCTGACTGAAAGAATGAATAACCCATCAAAATACGGTTTCAGGGAAGGAAAAGCGGCATGTTGTGGCTCGGGTCCATACAGAGGACGTCAGAACTGTGGAGGGAAGGGAGGTGTGGAAGAGTATGAATTATGTGACAATGTTACCGAATATGTTTTTTTTGACAATCATCCAACTGAAAGGGTTTACCATCAAGTTTCCAAGATATGGTGGAACCGAACTCCAAATGTCACAGGGCTTTACAATCTGAGATCTCTATTTGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

41.17

Weight (kDa)

7.98

Isoelectric Point (pI)

32.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 344 1.7e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 919
AccB7I CCANNNNNTGG 1 cut(s) 1035
AciI CCGC 1 cut(s) 884
AcsI RAATTY 3 cut(s) 184, 277, 635
AcyI GRCGYC 2 cut(s) 244, 916
AfiI CCNNNNNNNGG 2 cut(s) 911, 1035
AflIII ACRYGT 1 cut(s) 651
AgsI TTSAA 5 cut(s) 13, 421, 781, 802, 1091
AjnI CCWGG 2 cut(s) 30, 307
AjuI GAANNNNNNNTTGG 4 cut(s) 555, 587, 803, 835
AleI CACNNNNGTG 1 cut(s) 599
AluBI AGCT 8 cut(s) 66, 266, 368, 488, 549, 678, 725, 790
AluI AGCT 8 cut(s) 66, 266, 368, 488, 549, 678, 725, 790
Alw21I GWGCWC 2 cut(s) 364, 771
Ama87I CYCGRG 1 cut(s) 898
AoxI GGCC 1 cut(s) 191
ApeKI GCWGC 2 cut(s) 63, 104
ApoI RAATTY 3 cut(s) 184, 277, 635
AspS9I GGNCC 2 cut(s) 192, 902
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 5 cut(s) 134, 346, 467, 592, 748
AvaI CYCGRG 1 cut(s) 898
AvaII GGWCC 1 cut(s) 902
Bbv12I GWGCWC 2 cut(s) 364, 771
BbvI GCAGC 2 cut(s) 75, 116
BccI CCATC 5 cut(s) 68, 233, 452, 862, 1026
BciT130I CCWGG 2 cut(s) 32, 309
BclI TGATCA 1 cut(s) 603
BcnI CCSGG 1 cut(s) 254
BfaI CTAG 2 cut(s) 365, 452
BglII AGATCT 2 cut(s) 740, 1079
BisI GCNGC 3 cut(s) 64, 105, 885
BlsI GCNGC 3 cut(s) 65, 106, 886
Bme1390I CCNGG 3 cut(s) 32, 254, 309
Bme18I GGWCC 1 cut(s) 902
BmeT110I CYCGRG 1 cut(s) 898
BmgT120I GGNCC 2 cut(s) 192, 902
BmiI GGNNCC 4 cut(s) 511, 709, 903, 1043
BmrFI CCNGG 3 cut(s) 32, 254, 309
BmrI ACTGGG 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 93
BpuEI CTTGAG 1 cut(s) 540
BpuMI CCSGG 1 cut(s) 254
BsaHI GRCGYC 2 cut(s) 244, 916
BsaJI CCNNGG 2 cut(s) 308, 793
Bsc4I CCNNNNNNNGG 2 cut(s) 911, 1035
Bse1I ACTGG 3 cut(s) 88, 229, 738
BseBI CCWGG 2 cut(s) 32, 309
BseDI CCNNGG 2 cut(s) 308, 793
BseGI GGATG 2 cut(s) 27, 997
BseLI CCNNNNNNNGG 2 cut(s) 911, 1035
BseMII CTCAG 2 cut(s) 258, 1067
BseNI ACTGG 3 cut(s) 88, 229, 738
BseXI GCAGC 2 cut(s) 75, 116
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 1 cut(s) 193
BsiHKAI GWGCWC 2 cut(s) 364, 771
BsiHKCI CYCGRG 1 cut(s) 898
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 2 cut(s) 911, 1035
BsnI GGCC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 898
Bsp1286I GDGCHC 2 cut(s) 364, 771
Bsp143I GATC 3 cut(s) 603, 740, 1079
BspACI CCGC 1 cut(s) 884
BspANI GGCC 1 cut(s) 193
BspCNI CTCAG 2 cut(s) 259, 1068
BspHI TCATGA 1 cut(s) 70
BspLI GGNNCC 4 cut(s) 511, 709, 903, 1043
BspQI GCTCTTC 1 cut(s) 776
BsrI ACTGG 3 cut(s) 88, 229, 738
BssECI CCNNGG 2 cut(s) 308, 793
BssMI GATC 3 cut(s) 603, 740, 1079
BssNI GRCGYC 2 cut(s) 244, 916
BssT1I CCWWGG 1 cut(s) 793
Bst2UI CCWGG 2 cut(s) 32, 309
Bst4CI ACNGT 3 cut(s) 704, 866, 926
Bst6I CTCTTC 2 cut(s) 776, 942
BstACI GRCGYC 2 cut(s) 244, 916
BstC8I GCNNGC 2 cut(s) 46, 788
BstDEI CTNAG 2 cut(s) 267, 1076
BstF5I GGATG 2 cut(s) 27, 997
BstKTI GATC 3 cut(s) 606, 743, 1082
BstMBI GATC 3 cut(s) 603, 740, 1079
BstMWI GCNNNNNNNGC 2 cut(s) 41, 272
BstNI CCWGG 2 cut(s) 32, 309
BstNSI RCATGY 2 cut(s) 104, 891
BstSCI CCNGG 3 cut(s) 30, 252, 307
BstV1I GCAGC 2 cut(s) 75, 116
BstX2I RGATCY 2 cut(s) 740, 1079
BstYI RGATCY 2 cut(s) 740, 1079
BsuRI GGCC 1 cut(s) 193
BtsCI GGATG 2 cut(s) 27, 997
BtsIMutI CAGTG 2 cut(s) 709, 731
Cac8I GCNNGC 2 cut(s) 46, 788
CciI TCATGA 1 cut(s) 70
Cfr13I GGNCC 2 cut(s) 192, 902
CseI GACGC 1 cut(s) 233
CviAII CATG 4 cut(s) 71, 101, 577, 888
DdeI CTNAG 2 cut(s) 267, 1076
DpnI GATC 3 cut(s) 605, 742, 1081
DpnII GATC 3 cut(s) 603, 740, 1079
Eam1104I CTCTTC 2 cut(s) 776, 942
EarI CTCTTC 2 cut(s) 776, 942
Eco130I CCWWGG 1 cut(s) 793
Eco47I GGWCC 1 cut(s) 902
Eco88I CYCGRG 1 cut(s) 898
EcoRII CCWGG 2 cut(s) 30, 307
EcoT14I CCWWGG 1 cut(s) 793
ErhI CCWWGG 1 cut(s) 793
FaeI CATG 4 cut(s) 74, 104, 580, 891
FatI CATG 4 cut(s) 70, 100, 576, 887
FbaI TGATCA 1 cut(s) 603
Fnu4HI GCNGC 3 cut(s) 64, 105, 885
FokI GGATG 2 cut(s) 14, 984
Fsp4HI GCNGC 3 cut(s) 64, 105, 885
FspBI CTAG 2 cut(s) 365, 452
GluI GCNGC 3 cut(s) 64, 105, 885
HaeIII GGCC 1 cut(s) 193
HapII CCGG 1 cut(s) 254
HgaI GACGC 1 cut(s) 233
Hin1I GRCGYC 2 cut(s) 244, 916
Hin1II CATG 4 cut(s) 74, 104, 580, 891
HindIII AAGCTT 1 cut(s) 676
HinfI GANTC 5 cut(s) 88, 125, 203, 691, 798
HpaII CCGG 1 cut(s) 254
HphI GGTGA 5 cut(s) 134, 346, 467, 592, 748
Hpy166II GTNNAC 4 cut(s) 337, 493, 671, 1015
Hpy188I TCNGA 6 cut(s) 93, 215, 238, 608, 921, 1077
Hpy188III TCNNGA 4 cut(s) 71, 421, 557, 695
Hpy8I GTNNAC 4 cut(s) 337, 493, 671, 1015
HpyAV CCTTC 3 cut(s) 624, 869, 928
HpyCH4III ACNGT 3 cut(s) 704, 866, 926
HpyCH4IV ACGT 2 cut(s) 651, 916
HpyCH4V TGCA 4 cut(s) 44, 104, 275, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 272
HpyF3I CTNAG 2 cut(s) 267, 1076
HpySE526I ACGT 2 cut(s) 651, 916
Hsp92I GRCGYC 2 cut(s) 244, 916
Hsp92II CATG 4 cut(s) 74, 104, 580, 891
Ksp22I TGATCA 1 cut(s) 603
Kzo9I GATC 3 cut(s) 603, 740, 1079
LguI GCTCTTC 1 cut(s) 776
LmnI GCTCC 3 cut(s) 707, 774, 795
Lsp1109I GCAGC 2 cut(s) 75, 116
MaeI CTAG 2 cut(s) 365, 452
MaeII ACGT 2 cut(s) 651, 916
MaeIII GTNAC 5 cut(s) 654, 729, 962, 970, 1057
MalI GATC 3 cut(s) 605, 742, 1081
MboI GATC 3 cut(s) 603, 740, 1079
MboII GAAGA 3 cut(s) 575, 793, 959
MfeI CAATTG 2 cut(s) 407, 437
MflI RGATCY 2 cut(s) 740, 1079
MhlI GDGCHC 2 cut(s) 364, 771
MluCI AATT 8 cut(s) 184, 277, 407, 437, 498, 541, 635, 956
MmeI TCCRAC 2 cut(s) 121, 1025
MnlI CCTC 6 cut(s) 540, 602, 669, 905, 923, 932
MseI TTAA 1 cut(s) 398
MslI CAYNNNNRTG 1 cut(s) 599
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 3 cut(s) 32, 254, 309
MunI CAATTG 2 cut(s) 407, 437
MvaI CCWGG 2 cut(s) 32, 309
MwoI GCNNNNNNNGC 2 cut(s) 41, 272
NciI CCSGG 1 cut(s) 254
NdeII GATC 3 cut(s) 603, 740, 1079
NlaIII CATG 4 cut(s) 74, 104, 580, 891
NlaIV GGNNCC 4 cut(s) 511, 709, 903, 1043
NmuCI GTSAC 3 cut(s) 729, 962, 1057
NspI RCATGY 2 cut(s) 104, 891
OliI CACNNNNGTG 1 cut(s) 599
PagI TCATGA 1 cut(s) 70
PciSI GCTCTTC 1 cut(s) 776
PfeI GAWTC 5 cut(s) 88, 125, 203, 691, 798
PflMI CCANNNNNTGG 1 cut(s) 1035
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 3 cut(s) 65, 106, 886
Psp6I CCWGG 2 cut(s) 30, 307
PspGI CCWGG 2 cut(s) 30, 307
PspN4I GGNNCC 4 cut(s) 511, 709, 903, 1043
PspPI GGNCC 2 cut(s) 192, 902
PsuI RGATCY 2 cut(s) 740, 1079
PvuII CAGCTG 1 cut(s) 66
RseI CAYNNNNRTG 1 cut(s) 599
SapI GCTCTTC 1 cut(s) 776
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 3 cut(s) 64, 105, 885
Sau3AI GATC 3 cut(s) 603, 740, 1079
Sau96I GGNCC 2 cut(s) 192, 902
ScrFI CCNGG 3 cut(s) 32, 254, 309
SduI GDGCHC 2 cut(s) 364, 771
SinI GGWCC 1 cut(s) 902
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 1 cut(s) 555
SmoI CTYRAG 1 cut(s) 555
Sse9I AATT 8 cut(s) 184, 277, 407, 437, 498, 541, 635, 956
SsiI CCGC 1 cut(s) 884
SspI AATATT 1 cut(s) 806
SspMI CTAG 2 cut(s) 365, 452
StyD4I CCNGG 3 cut(s) 30, 252, 307
StyI CCWWGG 1 cut(s) 793
TaaI ACNGT 3 cut(s) 704, 866, 926
TaiI ACGT 2 cut(s) 654, 919
TaqI TCGA 1 cut(s) 315
TasI AATT 8 cut(s) 184, 277, 407, 437, 498, 541, 635, 956
TauI GCSGC 1 cut(s) 887
TfiI GAWTC 5 cut(s) 88, 125, 203, 691, 798
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TscAI CASTG 2 cut(s) 709, 738
TseFI GTSAC 3 cut(s) 729, 962, 1057
TseI GCWGC 2 cut(s) 63, 104
Tsp45I GTSAC 3 cut(s) 729, 962, 1057
TspDTI ATGAA 6 cut(s) 103, 551, 576, 731, 860, 969
TspRI CASTG 2 cut(s) 709, 738
Van91I CCANNNNNTGG 1 cut(s) 1035
VpaK11BI GGWCC 1 cut(s) 902
XapI RAATTY 3 cut(s) 184, 277, 635
XceI RCATGY 2 cut(s) 104, 891
XspI CTAG 2 cut(s) 365, 452
ZraI GACGTC 1 cut(s) 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.