Rh2AG456500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000193
Physical Location & Seq
Reverse (-)
67145975 .. 67167411
21437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG456500.1

Sequence Viewer

Length: 1254 bp
ATGAGAAGTTTATACGTACAAAAAAAAAGAATGGCAACTTCTTCAAGATTTCAAGTATATGTCCTGGCTTTTTGTGCAACCCTTCTTATTCAAAGTGGCTGTTATGGCCATTCTGTGCATCAGAGAAAACATACAGCCTTGTTCATCTTCGGGGATTCAACATTTGATGTTGGAAATAATAACTACATAAACACTTCCACTTTCTTTCAAGCAAATTTCTTCCCATATGGGGAAACCTTCTTCAGCCACCCGACTGGTAGGTTCTCCGATGGTCGTCTAATCCCAGATATCATTGCTGAATATGCAAACTTGCCAATGATTCCACCATACTTACAGCCGGGTTTCGACAACTATACTAATGGGGTGAACTTTGCATCTTCTGGGGCTGGTGTTCTAGCTGAAACTCATCAAGGATTTGTGTTGGACCTTAAAACTCAACTGGGTTATTTCAAAAATGTGGAGAGGCAGTTGAGGCACAGACTAGGTGAAGCAGAAGCTCACACATTGTTGTCCGAAGCTGTTTATTTGATTGCCATCGGAAGCGGTGATTACTCTTTCCCATTCATAGCGAATTCAAGTTTGTTCGAGTCTCACTCACATGAAGAATATGTTGGCATGGTGATAGGAAACCTTACAAATGTGATCAAAGAAATATACGAGAAAGGAGGAAGAAAATTTGGGATTAAAGGCATTGGGAATTTGGGTTGTATACCGGGCTTGAGAATAGTTAAACCAGGAAACACAAGCACCTGTAATGAAGAAGTAAATGCACTTTCAAAACTCCACAATAGAGTACTTGCTAAAGCCCTCCTGGAGCTGAAAGGACAGCTTCAAGACTTCATATACTCGAATCCAAATTTATACCCTTACGCAGATGACGTAGTTCATAATCCATCAAAATATGGTTTCAAGGAAGCAGAGATGGCATGCTGTGGCTCTGGTCCATTAAGAAGAATTAGTAGCTGCGGAGGGAAGCGAGGTGAGACTGAGTATCAGTTATGTGACAATGTTACCGACTATGTCTACTTTGATTCCGGCCATCCCACAGAAAGCTGCTTTTACAAAGTTGGCACTTACCACCATACTTACAACCTCGTAGACAATTATACTCAAAGGGTCAACTTTGCATCTGCTGGGGCTGGTTTCAAGGAAGGAAAGATGGCATGTTGTGGCTGTGGTCTATGCAGCGAAATAGGCAGCTGTGAAGGGAAGAGAAGTGTGACCGAGTACGAATTCTCCAACATTTGGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

417

Amino Acids

46.32

Weight (kDa)

6.7

Isoelectric Point (pI)

31.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 47 - 350 9.5e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1245
AccI GTMKAC 3 cut(s) 709, 1023, 1098
AciI CCGC 2 cut(s) 543, 966
AcoI YGGCCR 2 cut(s) 106, 1036
AcsI RAATTY 6 cut(s) 214, 571, 674, 697, 856, 1232
AcuI CTGAAG 1 cut(s) 226
AfaI GTAC 3 cut(s) 18, 795, 1229
AfiI CCNNNNNNNGG 2 cut(s) 229, 1245
AjnI CCWGG 3 cut(s) 63, 733, 810
AjuI GAANNNNNNNTTGG 2 cut(s) 594, 626
AluBI AGCT 8 cut(s) 398, 497, 518, 817, 829, 963, 1053, 1200
AluI AGCT 8 cut(s) 398, 497, 518, 817, 829, 963, 1053, 1200
Alw26I GTCTC 2 cut(s) 594, 977
AoxI GGCC 2 cut(s) 106, 1036
ApeKI GCWGC 4 cut(s) 963, 1053, 1185, 1197
ApoI RAATTY 6 cut(s) 214, 571, 674, 697, 856, 1232
AspS9I GGNCC 2 cut(s) 424, 941
AsuC2I CCSGG 2 cut(s) 339, 714
AsuHPI GGTGA 5 cut(s) 376, 497, 557, 631, 992
AvaII GGWCC 2 cut(s) 424, 941
BalI TGGCCA 1 cut(s) 108
BbvI GCAGC 4 cut(s) 950, 1040, 1197, 1209
BccI CCATC 6 cut(s) 263, 542, 901, 916, 1047, 1153
BciT130I CCWGG 3 cut(s) 65, 735, 812
BclI TGATCA 1 cut(s) 642
BcnI CCSGG 2 cut(s) 339, 714
BcoDI GTCTC 2 cut(s) 594, 977
BfaI CTAG 2 cut(s) 395, 482
BisI GCNGC 4 cut(s) 964, 1054, 1186, 1198
BlsI GCNGC 4 cut(s) 965, 1055, 1187, 1199
BmcAI AGTACT 1 cut(s) 795
Bme1390I CCNGG 5 cut(s) 65, 339, 714, 735, 812
Bme18I GGWCC 2 cut(s) 424, 941
BmgT120I GGNCC 2 cut(s) 424, 941
BmrFI CCNGG 5 cut(s) 65, 339, 714, 735, 812
BmrI ACTGGG 1 cut(s) 449
BmsI GCATC 3 cut(s) 127, 383, 1136
BmuI ACTGGG 1 cut(s) 449
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 1 cut(s) 833
BpuEI CTTGAG 1 cut(s) 739
BpuMI CCSGG 2 cut(s) 339, 714
BsaAI YACGTR 1 cut(s) 16
BsaBI GATNNNNATC 1 cut(s) 533
BsaXI ACNNNNNCTCC 4 cut(s) 452, 482, 1220, 1250
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 1245
Bse1I ACTGG 2 cut(s) 259, 444
Bse3DI GCAATG 1 cut(s) 291
Bse8I GATNNNNATC 1 cut(s) 533
BseBI CCWGG 3 cut(s) 65, 735, 812
BseGI GGATG 1 cut(s) 1039
BseJI GATNNNNATC 1 cut(s) 533
BseLI CCNNNNNNNGG 2 cut(s) 229, 1245
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 978
BseNI ACTGG 2 cut(s) 259, 444
BseXI GCAGC 4 cut(s) 950, 1040, 1197, 1209
BseYI CCCAGC 1 cut(s) 1133
BshFI GGCC 2 cut(s) 108, 1038
BsiSI CCGG 3 cut(s) 338, 713, 1035
BslI CCNNNNNNNGG 2 cut(s) 229, 1245
BsmAI GTCTC 2 cut(s) 594, 977
BsnI GGCC 2 cut(s) 108, 1038
Bsp143I GATC 1 cut(s) 642
BspACI CCGC 2 cut(s) 543, 966
BspANI GGCC 2 cut(s) 108, 1038
BspCNI CTCAG 1 cut(s) 979
BsrDI GCAATG 1 cut(s) 291
BsrI ACTGG 2 cut(s) 259, 444
BssMI GATC 1 cut(s) 642
BssNAI GTATAC 1 cut(s) 710
Bst1107I GTATAC 1 cut(s) 710
Bst2UI CCWGG 3 cut(s) 65, 735, 812
Bst6I CTCTTC 1 cut(s) 1205
BstBAI YACGTR 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 928
BstDEI CTNAG 1 cut(s) 987
BstF5I GGATG 1 cut(s) 1039
BstKTI GATC 1 cut(s) 645
BstMAI GTCTC 2 cut(s) 594, 977
BstMBI GATC 1 cut(s) 642
BstMWI GCNNNNNNNGC 6 cut(s) 74, 105, 302, 472, 923, 1194
BstNI CCWGG 3 cut(s) 65, 735, 812
BstNSI RCATGY 2 cut(s) 930, 1167
BstSCI CCNGG 5 cut(s) 63, 337, 712, 733, 810
BstSNI TACGTA 1 cut(s) 16
BstV1I GCAGC 4 cut(s) 950, 1040, 1197, 1209
BstXI CCANNNNNNTGG 1 cut(s) 254
BstZ17I GTATAC 1 cut(s) 710
BsuRI GGCC 2 cut(s) 108, 1038
BtsCI GGATG 1 cut(s) 1039
Cac8I GCNNGC 1 cut(s) 928
Cfr13I GGNCC 2 cut(s) 424, 941
Csp6I GTAC 3 cut(s) 17, 794, 1228
CviAII CATG 4 cut(s) 599, 616, 927, 1164
CviQI GTAC 3 cut(s) 17, 794, 1228
DdeI CTNAG 1 cut(s) 987
DpnI GATC 1 cut(s) 644
DpnII GATC 1 cut(s) 642
EaeI YGGCCR 2 cut(s) 106, 1036
Eam1104I CTCTTC 1 cut(s) 1205
EarI CTCTTC 1 cut(s) 1205
Eco105I TACGTA 1 cut(s) 16
Eco32I GATATC 1 cut(s) 289
Eco47I GGWCC 2 cut(s) 424, 941
Eco57I CTGAAG 1 cut(s) 226
EcoRI GAATTC 2 cut(s) 571, 1232
EcoRII CCWGG 3 cut(s) 63, 733, 810
EcoRV GATATC 1 cut(s) 289
FaeI CATG 4 cut(s) 602, 619, 930, 1167
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FatI CATG 4 cut(s) 598, 615, 926, 1163
FauNDI CATATG 1 cut(s) 226
FbaI TGATCA 1 cut(s) 642
FblI GTMKAC 3 cut(s) 709, 1023, 1098
Fnu4HI GCNGC 4 cut(s) 964, 1054, 1186, 1198
FokI GGATG 1 cut(s) 1026
Fsp4HI GCNGC 4 cut(s) 964, 1054, 1186, 1198
FspBI CTAG 2 cut(s) 395, 482
GluI GCNGC 4 cut(s) 964, 1054, 1186, 1198
GsaI CCCAGC 1 cut(s) 1137
GsuI CTGGAG 1 cut(s) 833
HaeIII GGCC 2 cut(s) 108, 1038
HapII CCGG 3 cut(s) 338, 713, 1035
Hin1II CATG 4 cut(s) 602, 619, 930, 1167
HincII GTYRAC 1 cut(s) 1120
HindII GTYRAC 1 cut(s) 1120
HinfI GANTC 5 cut(s) 155, 319, 587, 850, 1031
HpaII CCGG 3 cut(s) 338, 713, 1035
HphI GGTGA 5 cut(s) 376, 497, 557, 631, 992
Hpy166II GTNNAC 5 cut(s) 367, 710, 1024, 1099, 1120
Hpy188I TCNGA 4 cut(s) 123, 268, 514, 539
Hpy188III TCNNGA 3 cut(s) 45, 833, 1251
Hpy8I GTNNAC 5 cut(s) 367, 710, 1024, 1099, 1120
HpyAV CCTTC 4 cut(s) 92, 247, 1145, 1199
HpyCH4IV ACGT 2 cut(s) 15, 879
HpyCH4V TGCA 7 cut(s) 77, 118, 305, 374, 770, 1127, 1185
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 105, 302, 472, 923, 1194
HpyF3I CTNAG 1 cut(s) 987
HpySE526I ACGT 2 cut(s) 15, 879
Hsp92II CATG 4 cut(s) 602, 619, 930, 1167
Ksp22I TGATCA 1 cut(s) 642
Kzo9I GATC 1 cut(s) 642
LmnI GCTCC 1 cut(s) 814
Lsp1109I GCAGC 4 cut(s) 950, 1040, 1197, 1209
LweI GCATC 3 cut(s) 127, 383, 1136
MaeI CTAG 2 cut(s) 395, 482
MaeII ACGT 2 cut(s) 15, 879
MaeIII GTNAC 3 cut(s) 1001, 1009, 1219
MalI GATC 1 cut(s) 644
MboI GATC 1 cut(s) 642
MlsI TGGCCA 1 cut(s) 108
MluCI AATT 8 cut(s) 214, 571, 674, 697, 856, 954, 1102, 1232
MluNI TGGCCA 1 cut(s) 108
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 2 cut(s) 151, 402
MnlI CCTC 7 cut(s) 456, 465, 659, 818, 962, 971, 1103
Mox20I TGGCCA 1 cut(s) 108
MscI TGGCCA 1 cut(s) 108
MseI TTAA 4 cut(s) 429, 684, 729, 947
MslI CAYNNNNRTG 1 cut(s) 597
Msp20I TGGCCA 1 cut(s) 108
MspA1I CMGCKG 1 cut(s) 1200
MspI CCGG 3 cut(s) 338, 713, 1035
MspR9I CCNGG 5 cut(s) 65, 339, 714, 735, 812
MvaI CCWGG 3 cut(s) 65, 735, 812
MwoI GCNNNNNNNGC 6 cut(s) 74, 105, 302, 472, 923, 1194
NciI CCSGG 2 cut(s) 339, 714
NdeI CATATG 1 cut(s) 226
NdeII GATC 1 cut(s) 642
NlaIII CATG 4 cut(s) 602, 619, 930, 1167
NmuCI GTSAC 2 cut(s) 1001, 1219
NspI RCATGY 2 cut(s) 930, 1167
PaeI GCATGC 1 cut(s) 930
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PfeI GAWTC 4 cut(s) 155, 319, 850, 1031
PflFI GACNNNGTC 1 cut(s) 1019
PflMI CCANNNNNTGG 1 cut(s) 1245
PfoI TCCNGGA 1 cut(s) 810
PkrI GCNGC 4 cut(s) 965, 1055, 1187, 1199
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
Ppu21I YACGTR 1 cut(s) 16
Psp6I CCWGG 3 cut(s) 63, 733, 810
PspFI CCCAGC 1 cut(s) 1133
PspGI CCWGG 3 cut(s) 63, 733, 810
PspPI GGNCC 2 cut(s) 424, 941
PsyI GACNNNGTC 1 cut(s) 1019
PvuII CAGCTG 1 cut(s) 1200
RsaI GTAC 3 cut(s) 18, 795, 1229
RsaNI GTAC 3 cut(s) 17, 794, 1228
RseI CAYNNNNRTG 1 cut(s) 597
SaqAI TTAA 4 cut(s) 429, 684, 729, 947
SatI GCNGC 4 cut(s) 964, 1054, 1186, 1198
Sau3AI GATC 1 cut(s) 642
Sau96I GGNCC 2 cut(s) 424, 941
ScaI AGTACT 1 cut(s) 795
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 5 cut(s) 65, 339, 714, 735, 812
SfaNI GCATC 3 cut(s) 127, 383, 1136
SinI GGWCC 2 cut(s) 424, 941
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 1 cut(s) 718
SmoI CTYRAG 1 cut(s) 718
SnaBI TACGTA 1 cut(s) 16
SphI GCATGC 1 cut(s) 930
Sse9I AATT 8 cut(s) 214, 571, 674, 697, 856, 954, 1102, 1232
SsiI CCGC 2 cut(s) 543, 966
SspMI CTAG 2 cut(s) 395, 482
StyD4I CCNGG 5 cut(s) 63, 337, 712, 733, 810
TaiI ACGT 2 cut(s) 18, 882
TaqI TCGA 3 cut(s) 345, 585, 848
TaqII GACCGA 1 cut(s) 1238
TasI AATT 8 cut(s) 214, 571, 674, 697, 856, 954, 1102, 1232
TatI WGTACW 1 cut(s) 793
TfiI GAWTC 4 cut(s) 155, 319, 850, 1031
Tru1I TTAA 4 cut(s) 429, 684, 729, 947
Tru9I TTAA 4 cut(s) 429, 684, 729, 947
TseFI GTSAC 2 cut(s) 1001, 1219
TseI GCWGC 4 cut(s) 963, 1053, 1185, 1197
Tsp45I GTSAC 2 cut(s) 1001, 1219
TspDTI ATGAA 6 cut(s) 133, 553, 615, 771, 829, 875
Tth111I GACNNNGTC 1 cut(s) 1019
Van91I CCANNNNNTGG 1 cut(s) 1245
VpaK11BI GGWCC 2 cut(s) 424, 941
XapI RAATTY 6 cut(s) 214, 571, 674, 697, 856, 1232
XceI RCATGY 2 cut(s) 930, 1167
XmiI GTMKAC 3 cut(s) 709, 1023, 1098
XspI CTAG 2 cut(s) 395, 482
ZrmI AGTACT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.