RLG00000020262

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000193
Physical Location & Seq
Reverse (-)
62994533 .. 62997039
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020262

Sequence Viewer

Length: 585 bp
ATGTCGACTTCACTGTTTCAAGTTATAGACCTTAAAACTCAACTGGGTTATTTCAAGAAAGTGGAGAAGCAGTTGAAGCACAAACTAGGCCATGCAGAAGCCTACACATTGCTGACAGAAGCTGTTTACTTAATTGGCATTGGAGCCAATGACTATTTTTATGCACTCGGCACAAATTCCAGTTTGTTTGAGTCCTCACCTGAAGAATTTGTTGGCCTGGTGATAGGAAACCTGACAACTGTGATCAAGGAAATATATAAGAAAGGAGGAAGGAAATTTGGGTTTACAAGCATGGCTCCTCTGGGTTGTGTACCAAGCATGAGAGCAATGAAACCAGGAAACACAGGCACATGTTTCAAGGAGGGAAAGACGGCATGTTGTGGATCTGGTCCATACAGAGGAATTGGGAGTTGTGGAGGGAAGCGAGGCGTGACAGTGTATGAACTATGTGACAACGTTACTGAATATGTGTTTTTTGACTCCAATCATCCCACAGAAAGGAATGAGGATTCAAAGCCTAGTGGTCGACGGCGTCAGGATTCAAGGCTGCGATGGTGGCGGTGCGATGCTACATCCAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

195

Amino Acids

21.53

Weight (kDa)

9.21

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 14 - 119 7.4e-06 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 5, 526
AciI CCGC 1 cut(s) 559
AclI AACGTT 1 cut(s) 456
AclWI GGATC 1 cut(s) 391
AcsI RAATTY 3 cut(s) 175, 206, 275
AcuI CTGAAG 1 cut(s) 222
AcyI GRCGYC 1 cut(s) 532
AfaI GTAC 1 cut(s) 312
AfiI CCNNNNNNNGG 2 cut(s) 398, 498
AflIII ACRYGT 1 cut(s) 350
AgsI TTSAA 6 cut(s) 20, 55, 76, 358, 513, 543
AjnI CCWGG 2 cut(s) 216, 334
AjuI GAANNNNNNNTTGG 2 cut(s) 195, 227
AluBI AGCT 1 cut(s) 122
AluI AGCT 1 cut(s) 122
AlwI GGATC 1 cut(s) 391
AlwNI CAGNNNCTG 1 cut(s) 122
AoxI GGCC 2 cut(s) 88, 214
ApeKI GCWGC 1 cut(s) 547
ApoI RAATTY 3 cut(s) 175, 206, 275
AspS9I GGNCC 1 cut(s) 389
AsuHPI GGTGA 2 cut(s) 189, 232
AvaII GGWCC 1 cut(s) 389
BbvI GCAGC 1 cut(s) 534
BccI CCATC 1 cut(s) 546
BceAI ACGGC 2 cut(s) 387, 545
BciT130I CCWGG 2 cut(s) 218, 336
BclI TGATCA 1 cut(s) 243
BfaI CTAG 2 cut(s) 86, 519
BisI GCNGC 1 cut(s) 548
BlsI GCNGC 1 cut(s) 549
Bme1390I CCNGG 2 cut(s) 218, 336
Bme18I GGWCC 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 389
BmiI GGNNCC 2 cut(s) 145, 297
BmrFI CCNGG 2 cut(s) 218, 336
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 1 cut(s) 556
BmuI ACTGGG 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 532
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 498
Bse1I ACTGG 2 cut(s) 48, 180
Bse3DI GCAATG 2 cut(s) 107, 333
BseBI CCWGG 2 cut(s) 218, 336
BseGI GGATG 2 cut(s) 487, 572
BseLI CCNNNNNNNGG 2 cut(s) 398, 498
BseMI GCAATG 2 cut(s) 107, 333
BseNI ACTGG 2 cut(s) 48, 180
BseRI GAGGAG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 534
BshFI GGCC 2 cut(s) 90, 216
BslI CCNNNNNNNGG 2 cut(s) 398, 498
BsnI GGCC 2 cut(s) 90, 216
Bsp143I GATC 2 cut(s) 243, 383
BspACI CCGC 1 cut(s) 559
BspANI GGCC 2 cut(s) 90, 216
BspLI GGNNCC 2 cut(s) 145, 297
BspPI GGATC 1 cut(s) 391
BsrDI GCAATG 2 cut(s) 107, 333
BsrI ACTGG 2 cut(s) 48, 180
BssMI GATC 2 cut(s) 243, 383
BssNI GRCGYC 1 cut(s) 532
Bst2UI CCWGG 2 cut(s) 218, 336
Bst4CI ACNGT 3 cut(s) 15, 241, 436
BstACI GRCGYC 1 cut(s) 532
BstF5I GGATG 2 cut(s) 487, 572
BstKTI GATC 2 cut(s) 246, 386
BstMBI GATC 2 cut(s) 243, 383
BstMWI GCNNNNNNNGC 2 cut(s) 76, 556
BstNI CCWGG 2 cut(s) 218, 336
BstNSI RCATGY 2 cut(s) 354, 378
BstSCI CCNGG 2 cut(s) 216, 334
BstV1I GCAGC 1 cut(s) 534
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BsuRI GGCC 2 cut(s) 90, 216
BtgZI GCGATG 2 cut(s) 565, 579
BtsCI GGATG 2 cut(s) 487, 572
BtsIMutI CAGTG 2 cut(s) 11, 441
CaiI CAGNNNCTG 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 389
CseI GACGC 1 cut(s) 521
Csp6I GTAC 1 cut(s) 311
CviAII CATG 5 cut(s) 92, 292, 319, 351, 375
CviJI RGCY 8 cut(s) 90, 101, 122, 146, 216, 296, 517, 547
CviKI_1 RGCY 8 cut(s) 90, 101, 122, 146, 216, 296, 517, 547
CviQI GTAC 1 cut(s) 311
DpnI GATC 2 cut(s) 245, 385
DpnII GATC 2 cut(s) 243, 383
Eco47I GGWCC 1 cut(s) 389
Eco57I CTGAAG 1 cut(s) 222
EcoRII CCWGG 2 cut(s) 216, 334
FaeI CATG 5 cut(s) 95, 295, 322, 354, 378
FatI CATG 5 cut(s) 91, 291, 318, 350, 374
FbaI TGATCA 1 cut(s) 243
FblI GTMKAC 2 cut(s) 5, 526
Fnu4HI GCNGC 1 cut(s) 548
FokI GGATG 2 cut(s) 474, 559
Fsp4HI GCNGC 1 cut(s) 548
FspBI CTAG 2 cut(s) 86, 519
GluI GCNGC 1 cut(s) 548
HaeIII GGCC 2 cut(s) 90, 216
HgaI GACGC 1 cut(s) 521
Hin1I GRCGYC 1 cut(s) 532
Hin1II CATG 5 cut(s) 95, 295, 322, 354, 378
HincII GTYRAC 2 cut(s) 6, 527
HindII GTYRAC 2 cut(s) 6, 527
HinfI GANTC 4 cut(s) 191, 479, 509, 539
HphI GGTGA 2 cut(s) 189, 232
Hpy166II GTNNAC 5 cut(s) 6, 127, 285, 311, 527
Hpy188III TCNNGA 2 cut(s) 55, 536
Hpy8I GTNNAC 5 cut(s) 6, 127, 285, 311, 527
Hpy99I CGWCG 1 cut(s) 531
HpyAV CCTTC 1 cut(s) 264
HpyCH4III ACNGT 3 cut(s) 15, 241, 436
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 2 cut(s) 95, 164
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 556
HpySE526I ACGT 1 cut(s) 456
Hsp92I GRCGYC 1 cut(s) 532
Hsp92II CATG 5 cut(s) 95, 295, 322, 354, 378
Ksp22I TGATCA 1 cut(s) 243
Kzo9I GATC 2 cut(s) 243, 383
LmnI GCTCC 2 cut(s) 143, 301
Lsp1109I GCAGC 1 cut(s) 534
LweI GCATC 1 cut(s) 556
MaeI CTAG 2 cut(s) 86, 519
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 3 cut(s) 430, 449, 457
MalI GATC 2 cut(s) 245, 385
MboI GATC 2 cut(s) 243, 383
MboII GAAGA 1 cut(s) 215
MflI RGATCY 1 cut(s) 383
MluCI AATT 5 cut(s) 132, 175, 206, 275, 402
MlyI GAGTC 2 cut(s) 200, 473
MnlI CCTC 8 cut(s) 205, 260, 309, 355, 392, 410, 419, 499
MseI TTAA 2 cut(s) 33, 131
MspR9I CCNGG 2 cut(s) 218, 336
MvaI CCWGG 2 cut(s) 218, 336
MwoI GCNNNNNNNGC 2 cut(s) 76, 556
NdeII GATC 2 cut(s) 243, 383
NlaIII CATG 5 cut(s) 95, 295, 322, 354, 378
NlaIV GGNNCC 2 cut(s) 145, 297
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 2 cut(s) 430, 449
NspI RCATGY 2 cut(s) 354, 378
PciI ACATGT 1 cut(s) 350
PfeI GAWTC 2 cut(s) 509, 539
PflFI GACNNNGTC 1 cut(s) 531
PkrI GCNGC 1 cut(s) 549
PleI GAGTC 2 cut(s) 199, 473
PpsI GAGTC 2 cut(s) 199, 473
PscI ACATGT 1 cut(s) 350
Psp1406I AACGTT 1 cut(s) 456
Psp6I CCWGG 2 cut(s) 216, 334
PspGI CCWGG 2 cut(s) 216, 334
PspN4I GGNNCC 2 cut(s) 145, 297
PspPI GGNCC 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 122
PsuI RGATCY 1 cut(s) 383
PsyI GACNNNGTC 1 cut(s) 531
RsaI GTAC 1 cut(s) 312
RsaNI GTAC 1 cut(s) 311
SalI GTCGAC 2 cut(s) 4, 525
SaqAI TTAA 2 cut(s) 33, 131
SatI GCNGC 1 cut(s) 548
Sau3AI GATC 2 cut(s) 243, 383
Sau96I GGNCC 1 cut(s) 389
SchI GAGTC 2 cut(s) 200, 473
ScrFI CCNGG 2 cut(s) 218, 336
SetI ASST 5 cut(s) 33, 124, 202, 234, 459
SfaNI GCATC 1 cut(s) 556
SinI GGWCC 1 cut(s) 389
Sse9I AATT 5 cut(s) 132, 175, 206, 275, 402
SsiI CCGC 1 cut(s) 559
SspMI CTAG 2 cut(s) 86, 519
StyD4I CCNGG 2 cut(s) 216, 334
TaaI ACNGT 3 cut(s) 15, 241, 436
TaiI ACGT 1 cut(s) 459
TaqI TCGA 2 cut(s) 5, 526
TasI AATT 5 cut(s) 132, 175, 206, 275, 402
TfiI GAWTC 2 cut(s) 509, 539
Tru1I TTAA 2 cut(s) 33, 131
Tru9I TTAA 2 cut(s) 33, 131
TscAI CASTG 2 cut(s) 18, 441
TseFI GTSAC 2 cut(s) 430, 449
TseI GCWGC 1 cut(s) 547
Tsp45I GTSAC 2 cut(s) 430, 449
TspDTI ATGAA 2 cut(s) 344, 456
TspRI CASTG 2 cut(s) 18, 441
Tth111I GACNNNGTC 1 cut(s) 531
VpaK11BI GGWCC 1 cut(s) 389
XapI RAATTY 3 cut(s) 175, 206, 275
XceI RCATGY 2 cut(s) 354, 378
XmiI GTMKAC 2 cut(s) 5, 526
XspI CTAG 2 cut(s) 86, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.