Belongs to the 'GDSL' lipolytic enzyme family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 144 |
| AccI | GTMKAC | 1 cut(s) | 246 |
| AcsI | RAATTY | 4 cut(s) | 204, 308, 329, 347 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 144 |
| AgsI | TTSAA | 3 cut(s) | 49, 475, 691 |
| AjnI | CCWGG | 1 cut(s) | 406 |
| AluBI | AGCT | 2 cut(s) | 363, 389 |
| AluI | AGCT | 2 cut(s) | 363, 389 |
| AoxI | GGCC | 2 cut(s) | 135, 676 |
| ApoI | RAATTY | 4 cut(s) | 204, 308, 329, 347 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 507 |
| AspS9I | GGNCC | 2 cut(s) | 368, 548 |
| AsuHPI | GGTGA | 3 cut(s) | 75, 271, 355 |
| AsuII | TTCGAA | 1 cut(s) | 519 |
| AsuNHI | GCTAGC | 1 cut(s) | 4 |
| AvaII | GGWCC | 2 cut(s) | 368, 548 |
| BanII | GRGCYC | 1 cut(s) | 57 |
| BccI | CCATC | 3 cut(s) | 188, 334, 596 |
| BciT130I | CCWGG | 1 cut(s) | 408 |
| BciVI | GTATCC | 1 cut(s) | 183 |
| BfaI | CTAG | 4 cut(s) | 5, 209, 237, 491 |
| BfmI | CTRYAG | 1 cut(s) | 615 |
| BfuI | GTATCC | 1 cut(s) | 183 |
| Bme1390I | CCNGG | 1 cut(s) | 408 |
| Bme18I | GGWCC | 2 cut(s) | 368, 548 |
| BmgT120I | GGNCC | 2 cut(s) | 368, 548 |
| BmiI | GGNNCC | 1 cut(s) | 369 |
| BmrFI | CCNGG | 1 cut(s) | 408 |
| BmrI | ACTGGG | 1 cut(s) | 189 |
| BmsI | GCATC | 1 cut(s) | 82 |
| BmtI | GCTAGC | 1 cut(s) | 8 |
| BmuI | ACTGGG | 1 cut(s) | 189 |
| Bpu14I | TTCGAA | 1 cut(s) | 519 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 144 |
| Bse1I | ACTGG | 2 cut(s) | 149, 184 |
| Bse3DI | GCAATG | 2 cut(s) | 227, 274 |
| BseBI | CCWGG | 1 cut(s) | 408 |
| BseGI | GGATG | 3 cut(s) | 216, 345, 382 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 144 |
| BseMI | GCAATG | 2 cut(s) | 227, 274 |
| BseMII | CTCAG | 2 cut(s) | 146, 333 |
| BseNI | ACTGG | 2 cut(s) | 149, 184 |
| BshFI | GGCC | 2 cut(s) | 137, 678 |
| BsiSI | CCGG | 1 cut(s) | 96 |
| BslFI | GGGAC | 1 cut(s) | 381 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 144 |
| BsmFI | GGGAC | 1 cut(s) | 381 |
| BsnI | GGCC | 2 cut(s) | 137, 678 |
| Bsp119I | TTCGAA | 1 cut(s) | 519 |
| Bsp1286I | GDGCHC | 1 cut(s) | 57 |
| Bsp143I | GATC | 1 cut(s) | 495 |
| BspANI | GGCC | 2 cut(s) | 137, 678 |
| BspCNI | CTCAG | 2 cut(s) | 145, 332 |
| BspLI | GGNNCC | 1 cut(s) | 369 |
| BspOI | GCTAGC | 1 cut(s) | 8 |
| BspT104I | TTCGAA | 1 cut(s) | 519 |
| BsrDI | GCAATG | 2 cut(s) | 227, 274 |
| BsrI | ACTGG | 2 cut(s) | 149, 184 |
| BssMI | GATC | 1 cut(s) | 495 |
| Bst2UI | CCWGG | 1 cut(s) | 408 |
| Bst6I | CTCTTC | 1 cut(s) | 50 |
| BstBI | TTCGAA | 1 cut(s) | 519 |
| BstC8I | GCNNGC | 1 cut(s) | 6 |
| BstDEI | CTNAG | 3 cut(s) | 132, 319, 359 |
| BstF5I | GGATG | 3 cut(s) | 216, 345, 382 |
| BstKTI | GATC | 1 cut(s) | 498 |
| BstMBI | GATC | 1 cut(s) | 495 |
| BstNI | CCWGG | 1 cut(s) | 408 |
| BstSCI | CCNGG | 1 cut(s) | 406 |
| BstSFI | CTRYAG | 1 cut(s) | 615 |
| BsuI | GTATCC | 1 cut(s) | 183 |
| BsuRI | GGCC | 2 cut(s) | 137, 678 |
| BtsCI | GGATG | 3 cut(s) | 216, 345, 382 |
| BtsIMutI | CAGTG | 2 cut(s) | 177, 498 |
| Cac8I | GCNNGC | 1 cut(s) | 6 |
| Cfr13I | GGNCC | 2 cut(s) | 368, 548 |
| CviAII | CATG | 3 cut(s) | 427, 485, 674 |
| CviJI | RGCY | 7 cut(s) | 55, 137, 363, 389, 406, 560, 678 |
| CviKI_1 | RGCY | 7 cut(s) | 55, 137, 363, 389, 406, 560, 678 |
| DdeI | CTNAG | 3 cut(s) | 132, 319, 359 |
| DpnI | GATC | 1 cut(s) | 497 |
| DpnII | GATC | 1 cut(s) | 495 |
| Eam1104I | CTCTTC | 1 cut(s) | 50 |
| EarI | CTCTTC | 1 cut(s) | 50 |
| Eco24I | GRGCYC | 1 cut(s) | 57 |
| Eco47I | GGWCC | 2 cut(s) | 368, 548 |
| EcoRI | GAATTC | 2 cut(s) | 308, 329 |
| EcoRII | CCWGG | 1 cut(s) | 406 |
| EcoT38I | GRGCYC | 1 cut(s) | 57 |
| FaeI | CATG | 3 cut(s) | 430, 488, 677 |
| FaqI | GGGAC | 1 cut(s) | 381 |
| FatI | CATG | 3 cut(s) | 426, 484, 673 |
| FauNDI | CATATG | 1 cut(s) | 151 |
| FblI | GTMKAC | 1 cut(s) | 246 |
| FokI | GGATG | 3 cut(s) | 203, 352, 369 |
| FriOI | GRGCYC | 1 cut(s) | 57 |
| FspBI | CTAG | 4 cut(s) | 5, 209, 237, 491 |
| HaeIII | GGCC | 2 cut(s) | 137, 678 |
| HapII | CCGG | 1 cut(s) | 96 |
| Hin1II | CATG | 3 cut(s) | 430, 488, 677 |
| HindIII | AAGCTT | 1 cut(s) | 361 |
| HinfI | GANTC | 2 cut(s) | 80, 508 |
| HpaII | CCGG | 1 cut(s) | 96 |
| HphI | GGTGA | 3 cut(s) | 75, 271, 355 |
| Hpy166II | GTNNAC | 1 cut(s) | 247 |
| Hpy188I | TCNGA | 2 cut(s) | 322, 513 |
| Hpy188III | TCNNGA | 2 cut(s) | 209, 691 |
| Hpy8I | GTNNAC | 1 cut(s) | 247 |
| HpyAV | CCTTC | 2 cut(s) | 523, 689 |
| HpyCH4V | TGCA | 2 cut(s) | 289, 426 |
| HpyF3I | CTNAG | 3 cut(s) | 132, 319, 359 |
| Hsp92II | CATG | 3 cut(s) | 430, 488, 677 |
| Kzo9I | GATC | 1 cut(s) | 495 |
| LpnPI | CCDG | 9 cut(s) | 109, 119, 130, 165, 393, 399, 420, 644, 656 |
| LweI | GCATC | 1 cut(s) | 82 |
| MaeI | CTAG | 4 cut(s) | 5, 209, 237, 491 |
| MaeIII | GTNAC | 3 cut(s) | 354, 572, 669 |
| MalI | GATC | 1 cut(s) | 497 |
| MboI | GATC | 1 cut(s) | 495 |
| MboII | GAAGA | 5 cut(s) | 37, 335, 338, 443, 589 |
| MfeI | CAATTG | 2 cut(s) | 24, 372 |
| MhlI | GDGCHC | 1 cut(s) | 57 |
| MluCI | AATT | 9 cut(s) | 24, 204, 290, 308, 329, 347, 372, 468, 635 |
| MnlI | CCTC | 2 cut(s) | 294, 491 |
| MroXI | GAANNNNTTC | 1 cut(s) | 507 |
| MseI | TTAA | 1 cut(s) | 294 |
| MslI | CAYNNNNRTG | 1 cut(s) | 443 |
| MspI | CCGG | 1 cut(s) | 96 |
| MspR9I | CCNGG | 1 cut(s) | 408 |
| MunI | CAATTG | 2 cut(s) | 24, 372 |
| MvaI | CCWGG | 1 cut(s) | 408 |
| NdeI | CATATG | 1 cut(s) | 151 |
| NdeII | GATC | 1 cut(s) | 495 |
| NheI | GCTAGC | 1 cut(s) | 4 |
| NlaIII | CATG | 3 cut(s) | 430, 488, 677 |
| NlaIV | GGNNCC | 1 cut(s) | 369 |
| NmuCI | GTSAC | 2 cut(s) | 572, 669 |
| NspV | TTCGAA | 1 cut(s) | 519 |
| PdmI | GAANNNNTTC | 1 cut(s) | 507 |
| PfeI | GAWTC | 2 cut(s) | 80, 508 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 144 |
| Psp6I | CCWGG | 1 cut(s) | 406 |
| PspGI | CCWGG | 1 cut(s) | 406 |
| PspN4I | GGNNCC | 1 cut(s) | 369 |
| PspPI | GGNCC | 2 cut(s) | 368, 548 |
| RseI | CAYNNNNRTG | 1 cut(s) | 443 |
| SaqAI | TTAA | 1 cut(s) | 294 |
| Sau3AI | GATC | 1 cut(s) | 495 |
| Sau96I | GGNCC | 2 cut(s) | 368, 548 |
| ScrFI | CCNGG | 1 cut(s) | 408 |
| SduI | GDGCHC | 1 cut(s) | 57 |
| SetI | ASST | 5 cut(s) | 252, 365, 391, 534, 570 |
| SfaNI | GCATC | 1 cut(s) | 82 |
| SfcI | CTRYAG | 1 cut(s) | 615 |
| SfuI | TTCGAA | 1 cut(s) | 519 |
| SinI | GGWCC | 2 cut(s) | 368, 548 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 443 |
| Sse9I | AATT | 9 cut(s) | 24, 204, 290, 308, 329, 347, 372, 468, 635 |
| SspMI | CTAG | 4 cut(s) | 5, 209, 237, 491 |
| StyD4I | CCNGG | 1 cut(s) | 406 |
| TaqI | TCGA | 2 cut(s) | 519, 600 |
| TasI | AATT | 9 cut(s) | 24, 204, 290, 308, 329, 347, 372, 468, 635 |
| TfiI | GAWTC | 2 cut(s) | 80, 508 |
| Tru1I | TTAA | 1 cut(s) | 294 |
| Tru9I | TTAA | 1 cut(s) | 294 |
| TscAI | CASTG | 2 cut(s) | 184, 505 |
| TseFI | GTSAC | 2 cut(s) | 572, 669 |
| Tsp45I | GTSAC | 2 cut(s) | 572, 669 |
| TspDTI | ATGAA | 1 cut(s) | 205 |
| TspRI | CASTG | 2 cut(s) | 184, 505 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 144 |
| VpaK11BI | GGWCC | 2 cut(s) | 368, 548 |
| XapI | RAATTY | 4 cut(s) | 204, 308, 329, 347 |
| XbaI | TCTAGA | 1 cut(s) | 208 |
| XmiI | GTMKAC | 1 cut(s) | 246 |
| XmnI | GAANNNNTTC | 1 cut(s) | 507 |
| XspI | CTAG | 4 cut(s) | 5, 209, 237, 491 |