RLG00000020252

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000193
Physical Location & Seq
Reverse (-)
62887100 .. 62889191
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020252

Sequence Viewer

Length: 795 bp
ATGTATACTACACCCAGGGACAAAAATACAGAGAAGAAGGATAAGGATCAAACACGAGGAACACGAATTGTGTTGGACCTTAAAACTCAACTGGGTTATTTCAAAAATGTGGAGAAGCAGTTGAGGCACAGACTAGGTGAAGCAGAAGCTCACACATTGTTGTCCGAAGCTGTTTACTTGATTGCCATCGGAAGCGGTGATTACTCTTTCCCATTCATAGCGAATTCAAGTTTGTTCGAGTCTCACTCACATGAAGAATATGTTGGCATGGTGATAGGAAACCTTACAAATGTGATCAAAGAAATATACGAGAAAGGAGGAAGAAAATTTGGGATTAAAGGCATAGGGAATTTGGGTTGTATACCGGGCTTGAGAATAGTTAAACCAGGAAACACAAGCACCTGTAATGAAGAAGTAAATGCCCTTTCAAAACTCCACAATAGAGTACTTGCTAAAGCCCTCCTGGAGCTGAAAGGACAGCTTCAAGACTTCATATACTCGAATCCAAATTTATACTCTTACGCAGATGACGTAGTTCATAATCCATCAAAATATGGTTTCAAGGAAGCAGAGATGGCATGCTGTGGCTCTGGTCCATTAAGAAGAATTAGTAGCTGCGGAGGGAAGCGAGGTGTGACTGAGTATCAGTTATGTGACAATGTTACTGACTATGTCTACTTTGATTCCGGCCATCCCACAGAAAGGTATAACCAGAAACTTTCCAAGTTATGGTGGAGCCATACTCCTGATGTCACAGGACGTTACATCAATTTGAAAGAGCTATTTGAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

265

Amino Acids

29.77

Weight (kDa)

8.4

Isoelectric Point (pI)

22.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 27 - 243 5.5e-11 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 729
AccI GTMKAC 3 cut(s) 5, 361, 675
AciI CCGC 2 cut(s) 195, 618
AclWI GGATC 1 cut(s) 54
AcoI YGGCCR 1 cut(s) 688
AcsI RAATTY 4 cut(s) 223, 326, 349, 508
AfaI GTAC 1 cut(s) 447
AfiI CCNNNNNNNGG 2 cut(s) 702, 729
AgsI TTSAA 7 cut(s) 103, 228, 429, 485, 562, 775, 788
AjnI CCWGG 3 cut(s) 14, 385, 462
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 6 cut(s) 149, 170, 469, 481, 615, 781
AluI AGCT 6 cut(s) 149, 170, 469, 481, 615, 781
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 1 cut(s) 54
AoxI GGCC 1 cut(s) 688
ApeKI GCWGC 1 cut(s) 615
ApoI RAATTY 4 cut(s) 223, 326, 349, 508
AspS9I GGNCC 2 cut(s) 76, 593
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 3 cut(s) 149, 209, 283
AvaII GGWCC 2 cut(s) 76, 593
BauI CACGAG 1 cut(s) 54
BbvI GCAGC 1 cut(s) 602
BccI CCATC 4 cut(s) 194, 553, 568, 699
BciT130I CCWGG 3 cut(s) 16, 387, 464
BclI TGATCA 1 cut(s) 294
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 1 cut(s) 134
BisI GCNGC 1 cut(s) 616
BlsI GCNGC 1 cut(s) 617
BmcAI AGTACT 1 cut(s) 447
Bme1390I CCNGG 4 cut(s) 16, 366, 387, 464
Bme18I GGWCC 2 cut(s) 76, 593
BmgT120I GGNCC 2 cut(s) 76, 593
BmiI GGNNCC 1 cut(s) 737
BmrFI CCNGG 4 cut(s) 16, 366, 387, 464
BmrI ACTGGG 1 cut(s) 101
BmuI ACTGGG 1 cut(s) 101
BplI GAGNNNNNCTC 4 cut(s) 230, 262, 727, 759
BpmI CTGGAG 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 391
BpuMI CCSGG 1 cut(s) 366
BsaBI GATNNNNATC 2 cut(s) 45, 185
BsaJI CCNNGG 2 cut(s) 14, 15
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 2 cut(s) 702, 729
Bse1I ACTGG 1 cut(s) 96
Bse8I GATNNNNATC 2 cut(s) 45, 185
BseBI CCWGG 3 cut(s) 16, 387, 464
BseDI CCNNGG 2 cut(s) 14, 15
BseGI GGATG 1 cut(s) 691
BseJI GATNNNNATC 2 cut(s) 45, 185
BseLI CCNNNNNNNGG 2 cut(s) 702, 729
BseMII CTCAG 1 cut(s) 630
BseNI ACTGG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 602
BshFI GGCC 1 cut(s) 690
BsiSI CCGG 2 cut(s) 365, 687
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 2 cut(s) 702, 729
BsmAI GTCTC 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 32
BsnI GGCC 1 cut(s) 690
Bsp143I GATC 2 cut(s) 46, 294
BspACI CCGC 2 cut(s) 195, 618
BspANI GGCC 1 cut(s) 690
BspCNI CTCAG 1 cut(s) 631
BspLI GGNNCC 1 cut(s) 737
BspPI GGATC 1 cut(s) 54
BsrI ACTGG 1 cut(s) 96
BssECI CCNNGG 2 cut(s) 14, 15
BssMI GATC 2 cut(s) 46, 294
BssNAI GTATAC 2 cut(s) 6, 362
BssSI CACGAG 1 cut(s) 54
Bst1107I GTATAC 2 cut(s) 6, 362
Bst2BI CACGAG 1 cut(s) 54
Bst2UI CCWGG 3 cut(s) 16, 387, 464
BstC8I GCNNGC 1 cut(s) 580
BstDEI CTNAG 1 cut(s) 639
BstF5I GGATG 1 cut(s) 691
BstKTI GATC 2 cut(s) 49, 297
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 2 cut(s) 46, 294
BstMWI GCNNNNNNNGC 2 cut(s) 124, 575
BstNI CCWGG 3 cut(s) 16, 387, 464
BstNSI RCATGY 1 cut(s) 582
BstSCI CCNGG 4 cut(s) 14, 364, 385, 462
BstV1I GCAGC 1 cut(s) 602
BstZ17I GTATAC 2 cut(s) 6, 362
BsuRI GGCC 1 cut(s) 690
BtsCI GGATG 1 cut(s) 691
Cac8I GCNNGC 1 cut(s) 580
Cfr13I GGNCC 2 cut(s) 76, 593
Csp6I GTAC 1 cut(s) 446
CviAII CATG 3 cut(s) 251, 268, 579
CviQI GTAC 1 cut(s) 446
DdeI CTNAG 1 cut(s) 639
DpnI GATC 2 cut(s) 48, 296
DpnII GATC 2 cut(s) 46, 294
EaeI YGGCCR 1 cut(s) 688
Eco47I GGWCC 2 cut(s) 76, 593
EcoRI GAATTC 1 cut(s) 223
EcoRII CCWGG 3 cut(s) 14, 385, 462
FaeI CATG 3 cut(s) 254, 271, 582
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FaqI GGGAC 1 cut(s) 32
FatI CATG 3 cut(s) 250, 267, 578
FbaI TGATCA 1 cut(s) 294
FblI GTMKAC 3 cut(s) 5, 361, 675
Fnu4HI GCNGC 1 cut(s) 616
FokI GGATG 1 cut(s) 678
Fsp4HI GCNGC 1 cut(s) 616
FspBI CTAG 1 cut(s) 134
GluI GCNGC 1 cut(s) 616
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 1 cut(s) 690
HapII CCGG 2 cut(s) 365, 687
Hin1II CATG 3 cut(s) 254, 271, 582
HinfI GANTC 3 cut(s) 239, 502, 683
HpaII CCGG 2 cut(s) 365, 687
HphI GGTGA 3 cut(s) 149, 209, 283
Hpy166II GTNNAC 4 cut(s) 6, 175, 362, 676
Hpy188I TCNGA 2 cut(s) 166, 191
Hpy188III TCNNGA 2 cut(s) 485, 746
Hpy8I GTNNAC 4 cut(s) 6, 175, 362, 676
HpyAV CCTTC 1 cut(s) 31
HpyCH4IV ACGT 2 cut(s) 531, 760
HpyF10VI GCNNNNNNNGC 2 cut(s) 124, 575
HpyF3I CTNAG 1 cut(s) 639
HpySE526I ACGT 2 cut(s) 531, 760
Hsp92II CATG 3 cut(s) 254, 271, 582
Ksp22I TGATCA 1 cut(s) 294
Kzo9I GATC 2 cut(s) 46, 294
LmnI GCTCC 2 cut(s) 466, 735
Lsp1109I GCAGC 1 cut(s) 602
MaeI CTAG 1 cut(s) 134
MaeII ACGT 2 cut(s) 531, 760
MaeIII GTNAC 5 cut(s) 634, 653, 661, 751, 761
MalI GATC 2 cut(s) 48, 296
MboI GATC 2 cut(s) 46, 294
MboII GAAGA 5 cut(s) 46, 266, 333, 422, 615
MluCI AATT 7 cut(s) 66, 223, 326, 349, 508, 606, 769
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 6 cut(s) 50, 117, 311, 470, 614, 623
MseI TTAA 4 cut(s) 81, 336, 381, 599
MslI CAYNNNNRTG 1 cut(s) 249
MspI CCGG 2 cut(s) 365, 687
MspR9I CCNGG 4 cut(s) 16, 366, 387, 464
MvaI CCWGG 3 cut(s) 16, 387, 464
MwoI GCNNNNNNNGC 2 cut(s) 124, 575
NciI CCSGG 1 cut(s) 366
NdeII GATC 2 cut(s) 46, 294
NlaIII CATG 3 cut(s) 254, 271, 582
NlaIV GGNNCC 1 cut(s) 737
NmuCI GTSAC 3 cut(s) 634, 653, 751
NspI RCATGY 1 cut(s) 582
PaeI GCATGC 1 cut(s) 582
PasI CCCWGGG 1 cut(s) 15
PcsI WCGNNNNNNNCGW 2 cut(s) 61, 528
PfeI GAWTC 2 cut(s) 502, 683
PflFI GACNNNGTC 1 cut(s) 671
PflMI CCANNNNNTGG 1 cut(s) 729
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 1 cut(s) 617
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
Psp6I CCWGG 3 cut(s) 14, 385, 462
PspGI CCWGG 3 cut(s) 14, 385, 462
PspN4I GGNNCC 1 cut(s) 737
PspPI GGNCC 2 cut(s) 76, 593
PsyI GACNNNGTC 1 cut(s) 671
RsaI GTAC 1 cut(s) 447
RsaNI GTAC 1 cut(s) 446
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 4 cut(s) 81, 336, 381, 599
SatI GCNGC 1 cut(s) 616
Sau3AI GATC 2 cut(s) 46, 294
Sau96I GGNCC 2 cut(s) 76, 593
ScaI AGTACT 1 cut(s) 447
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 4 cut(s) 16, 366, 387, 464
SinI GGWCC 2 cut(s) 76, 593
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
SphI GCATGC 1 cut(s) 582
Sse9I AATT 7 cut(s) 66, 223, 326, 349, 508, 606, 769
SsiI CCGC 2 cut(s) 195, 618
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 4 cut(s) 14, 364, 385, 462
TaiI ACGT 2 cut(s) 534, 763
TaqI TCGA 2 cut(s) 237, 500
TasI AATT 7 cut(s) 66, 223, 326, 349, 508, 606, 769
TatI WGTACW 1 cut(s) 445
TfiI GAWTC 2 cut(s) 502, 683
Tru1I TTAA 4 cut(s) 81, 336, 381, 599
Tru9I TTAA 4 cut(s) 81, 336, 381, 599
TseFI GTSAC 3 cut(s) 634, 653, 751
TseI GCWGC 1 cut(s) 615
Tsp45I GTSAC 3 cut(s) 634, 653, 751
TspDTI ATGAA 5 cut(s) 205, 267, 423, 481, 527
Tth111I GACNNNGTC 1 cut(s) 671
Van91I CCANNNNNTGG 1 cut(s) 729
VpaK11BI GGWCC 2 cut(s) 76, 593
XapI RAATTY 4 cut(s) 223, 326, 349, 508
XceI RCATGY 1 cut(s) 582
XmiI GTMKAC 3 cut(s) 5, 361, 675
XspI CTAG 1 cut(s) 134
ZrmI AGTACT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.