Rh2BG469000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000193
Physical Location & Seq
Reverse (-)
66217193 .. 66249446
32254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG469000.1

Sequence Viewer

Length: 1161 bp
ATGTATAGATCTCAAACAGATGAGAAGTTTATACGTACAGCAAAAAGAATGACAACTTCTTCAAGATTTCAAGTATATGTCCTGGCTTTTTGTGCAACCCTTCTTATTCAAAGTGGCTGTTATGGCCATTCTGTGCATCAGAGAAAACATACAGCCTTGTTCATCTTCGGGGATTCAACATTTGATGTTGGAAATAATAACTACATAAACACTTCCACTTTCTTTCAAGCAAATTTCTTCCCATATGGGGAAACCTTCTTCAGCCACCCGACTGGTAGGTTCTCCGATGGTCGTCTAATCCCAGATATCATTGCTGAATATGCAAACTTGCCAATGATTCCACCATACTTACAGCCGGGTTTCGACAACTATACTAATGGGGTGAACTTTGCATCTTCTGGGGCTGGTGTTCTAGCTGAAACTCATCAAGGATTTGTGTTGGACCTTAAAACTCAACTGGGTTATTTCAAAAATGTGGAGAAGCAGTTGAGGCACAGACTAGGTGAAGCAGAAGCTCACACGTTGTTGTCCGAAGCTGTTTACTTGATTGCCATCGGAAGCGGTGATTACTCTTTCCCATTCATAGCGAATTCAAGTTTGTTCGAGTCTCACTCACATGAAGAATATGTTGGCATGGTGATAGGAAACCTTACAAATGTGATCAAAGAAATATACGAGAAAGGAGGAAGAAGATTTGGGATTAAAGGCATTGGGAATTTGGGTTGTATACCGGGCTTGAGAATAGTTAAACCAGGAAACACAAGCACCTGTAATGAAGAAGTAAATGCACTTTCAAAACTCCACAATAGAGTACTTGCTAAAGCCCTCCTGGAGCTGAAAGGACAGCTTCAAGACTTCATATACTCGAATCCAAATTTATACCCTTACGCAGATGACGTAGTTCATAATCCATCAAAATATGGTTTCAAGGAAGCAGAGATGGCATGCTGTGGCTCTGGTCCATTAAGAAGAATTAGTAGCTGCGGAGGGAAGCGAGGTGTGACTGAGTATCAGTTATGTGACAATGTTACTGACTATGTCTACTTTGATTCCGGCCATCCCACAGAAAGGTATAACCAGAAACTTTCGAAGTTATGGTGGAGCCATACTCCTGATGTCACAGGACGTTACATCAATTTGAAAGAGCTATTTGAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

386

Amino Acids

43.35

Weight (kDa)

7.19

Isoelectric Point (pI)

27.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 53 - 365 9.4e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53940 AT1G53940 AT1G53970 AT1G53970 AT1G53990 AT3G14225 AT3G14225 AT5G40990
fragaria_vesca FvH4_6g34510 FvH4_6g35571 FvH4_6g35571 FvH4_6g35572 FvH4_6g35580 FvH4_6g35580 FvH4_6g35584 FvH4_6g35600 FvH4_6g35610 FvH4_6g35630 FvH4_6g35631
malus_domestica MD00G1137100.v1.1 MD01G1122400.v1.1 MD07G1191900.v1.1 MD09G1174600.v1.1 MD09G1174700.v1.1 MD09G1175100.v1.1 MD17G1151700.v1.1 MD17G1151800.v1.1
prunus_persica Prupe.3G032700_v2.0.a1 Prupe.3G032900_v2.0.a1 Prupe.3G033100_v2.0.a1 Prupe.3G033200_v2.0.a1 Prupe.3G033300_v2.0.a1 Prupe.3G033400_v2.0.a1 Prupe.3G033500_v2.0.a1 Prupe.3G033600_v2.0.a1
pyrus_communis pycom01g14930 pycom09g09170 pycom09g09180 pycom09g09190 pycom09g09220 pycom17g14510
rosa_chinensis RchiOBHm_Chr2g0147161 RchiOBHm_Chr2g0147301 RchiOBHm_Chr2g0147311 RchiOBHm_Chr2g0147391 RchiOBHm_Chr2g0147431 RchiOBHm_Chr2g0147441 RchiOBHm_Chr2g0147471 RchiOBHm_Chr2g0147491 RchiOBHm_Chr2g0147591 RchiOBHm_Chr2g0147911 RchiOBHm_Chr2g0148061 RchiOBHm_Chr4g0407441 RchiOBHm_Chr4g0407471 RchiOBHm_Chr6g0252881 RchiOBHm_Chr7g0228391 RchiOBHm_Chr7g0228411
rosa_laevigata RLG00000001566 RLG00000020247 RLG00000020250 RLG00000020251 RLG00000020252 RLG00000020255 RLG00000020258 RLG00000020261 RLG00000020262 RLG00000020264 RLG00000020293 RLG00000020307 RLG00000030814
rosa_multiflora Rmu_co8112460.1_g000001 Rmu_co8353483.1_g000001 Rmu_co8492209.1_g000001 Rmu_sc0000308.1_g000061 Rmu_sc0000538.1_g000003 Rmu_sc0001159.1_g000017 Rmu_sc0001159.1_g000023 Rmu_sc0001159.1_g000050 Rmu_sc0001880.1_g000011 Rmu_sc0006302.1_g000003 Rmu_sc0008083.1_g000003 Rmu_sc0010661.1_g000002 Rmu_sc0017091.1_g000001 Rmu_sc0021146.1_g000001
rosa_roxburghii Rroxscaffold_2G00099470 Rroxscaffold_2G00099500 Rroxscaffold_2G00099530 Rroxscaffold_2G00099590 Rroxscaffold_2G00113160 Rroxscaffold_2G00113180 Rroxscaffold_3G00231150 Rroxscaffold_4G00332370 Rroxscaffold_5G00352070
rosa_rugosa Rorug02G0398800 Rorug02G0399100 Rorug02G0399200 Rorug02G0399300 Rorug02G0400100 Rorug02G0400200 Rorug02G0400300.1 Rorug02G0403000 Rorug05G0546600 Rorug05G0547200 Rorug07G0249100 Rorug07G0249200.1 RorugPtG0001700.1
rosa_samantha Rh1CG003800 Rh2AG456000 Rh2AG456200 Rh2AG456400 Rh2AG456500 Rh2AG456900 Rh2AG457200 Rh2AG457500 Rh2AG457600 Rh2AG457700 Rh2AG460000 Rh2AG460400 Rh2BG468900 Rh2BG469000 Rh2BG469800 Rh2BG469900 Rh2BG470000 Rh2BG473300 Rh2BG473800 Rh2CG443400 Rh2CG443800 Rh2CG443900 Rh2CG444400 Rh2CG444600 Rh2CG445000 Rh2CG445100 Rh2CG447300 Rh2CG447600 Rh2DG478200 Rh2DG478300 Rh2DG479300 Rh2DG479500 Rh2DG482200 Rh4AG048300 Rh4CG051600 Rh4CG051700 Rh4CG151200 Rh7AG401800 Rh7CG420700 Rh7DG397900
rosa_wichuraiana Rw2G037300 Rw2G037330 Rw2G037350 Rw2G037360 Rw2G037390 Rw2G037410 Rw2G037620 Rw4G003830 Rw6G005020 Rw6G005580 Rw7G033400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 727, 1041
AciI CCGC 2 cut(s) 561, 984
AcoI YGGCCR 2 cut(s) 124, 1054
AcsI RAATTY 4 cut(s) 232, 589, 715, 874
AcuI CTGAAG 1 cut(s) 244
AfaI GTAC 2 cut(s) 37, 813
AfiI CCNNNNNNNGG 2 cut(s) 247, 1068
AflIII ACRYGT 1 cut(s) 519
AjnI CCWGG 3 cut(s) 81, 751, 828
AjuI GAANNNNNNNTTGG 2 cut(s) 612, 644
AluBI AGCT 7 cut(s) 416, 515, 536, 835, 847, 981, 1147
AluI AGCT 7 cut(s) 416, 515, 536, 835, 847, 981, 1147
Alw26I GTCTC 1 cut(s) 612
AoxI GGCC 2 cut(s) 124, 1054
ApeKI GCWGC 1 cut(s) 981
ApoI RAATTY 4 cut(s) 232, 589, 715, 874
AspS9I GGNCC 2 cut(s) 442, 959
AsuC2I CCSGG 2 cut(s) 357, 732
AsuHPI GGTGA 4 cut(s) 394, 515, 575, 649
AsuII TTCGAA 1 cut(s) 1088
AvaII GGWCC 2 cut(s) 442, 959
BalI TGGCCA 1 cut(s) 126
BbvI GCAGC 1 cut(s) 968
BccI CCATC 5 cut(s) 281, 560, 919, 934, 1065
BciT130I CCWGG 3 cut(s) 83, 753, 830
BclI TGATCA 1 cut(s) 660
BcnI CCSGG 2 cut(s) 357, 732
BcoDI GTCTC 1 cut(s) 612
BfaI CTAG 2 cut(s) 413, 500
BglII AGATCT 1 cut(s) 8
BisI GCNGC 1 cut(s) 982
BlsI GCNGC 1 cut(s) 983
BmcAI AGTACT 1 cut(s) 813
Bme1390I CCNGG 5 cut(s) 83, 357, 732, 753, 830
Bme18I GGWCC 2 cut(s) 442, 959
BmgT120I GGNCC 2 cut(s) 442, 959
BmiI GGNNCC 1 cut(s) 1103
BmrFI CCNGG 5 cut(s) 83, 357, 732, 753, 830
BmrI ACTGGG 1 cut(s) 467
BmsI GCATC 2 cut(s) 145, 401
BmuI ACTGGG 1 cut(s) 467
BplI GAGNNNNNCTC 4 cut(s) 596, 628, 1093, 1125
BpmI CTGGAG 1 cut(s) 851
Bpu14I TTCGAA 1 cut(s) 1088
BpuEI CTTGAG 1 cut(s) 757
BpuMI CCSGG 2 cut(s) 357, 732
BsaAI YACGTR 1 cut(s) 35
BsaBI GATNNNNATC 1 cut(s) 551
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 1068
Bse1I ACTGG 2 cut(s) 277, 462
Bse3DI GCAATG 1 cut(s) 309
Bse8I GATNNNNATC 1 cut(s) 551
BseBI CCWGG 3 cut(s) 83, 753, 830
BseGI GGATG 1 cut(s) 1057
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 2 cut(s) 247, 1068
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 1 cut(s) 996
BseNI ACTGG 2 cut(s) 277, 462
BseXI GCAGC 1 cut(s) 968
BshFI GGCC 2 cut(s) 126, 1056
BsiSI CCGG 3 cut(s) 356, 731, 1053
BslI CCNNNNNNNGG 2 cut(s) 247, 1068
BsmAI GTCTC 1 cut(s) 612
BsnI GGCC 2 cut(s) 126, 1056
Bsp119I TTCGAA 1 cut(s) 1088
Bsp143I GATC 2 cut(s) 8, 660
BspACI CCGC 2 cut(s) 561, 984
BspANI GGCC 2 cut(s) 126, 1056
BspCNI CTCAG 1 cut(s) 997
BspLI GGNNCC 1 cut(s) 1103
BspT104I TTCGAA 1 cut(s) 1088
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 2 cut(s) 277, 462
BssMI GATC 2 cut(s) 8, 660
BssNAI GTATAC 1 cut(s) 728
Bst1107I GTATAC 1 cut(s) 728
Bst2UI CCWGG 3 cut(s) 83, 753, 830
BstBAI YACGTR 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 1088
BstC8I GCNNGC 1 cut(s) 946
BstDEI CTNAG 1 cut(s) 1005
BstF5I GGATG 1 cut(s) 1057
BstKTI GATC 2 cut(s) 11, 663
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 2 cut(s) 8, 660
BstMWI GCNNNNNNNGC 5 cut(s) 92, 123, 320, 490, 941
BstNI CCWGG 3 cut(s) 83, 753, 830
BstNSI RCATGY 1 cut(s) 948
BstSCI CCNGG 5 cut(s) 81, 355, 730, 751, 828
BstSNI TACGTA 1 cut(s) 35
BstV1I GCAGC 1 cut(s) 968
BstX2I RGATCY 1 cut(s) 8
BstXI CCANNNNNNTGG 1 cut(s) 272
BstYI RGATCY 1 cut(s) 8
BstZ17I GTATAC 1 cut(s) 728
BsuRI GGCC 2 cut(s) 126, 1056
BtsCI GGATG 1 cut(s) 1057
Cac8I GCNNGC 1 cut(s) 946
Cfr13I GGNCC 2 cut(s) 442, 959
Csp6I GTAC 2 cut(s) 36, 812
CviAII CATG 3 cut(s) 617, 634, 945
CviQI GTAC 2 cut(s) 36, 812
DdeI CTNAG 1 cut(s) 1005
DpnI GATC 2 cut(s) 10, 662
DpnII GATC 2 cut(s) 8, 660
EaeI YGGCCR 2 cut(s) 124, 1054
Eco105I TACGTA 1 cut(s) 35
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 2 cut(s) 442, 959
Eco57I CTGAAG 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 589
EcoRII CCWGG 3 cut(s) 81, 751, 828
EcoRV GATATC 1 cut(s) 307
FaeI CATG 3 cut(s) 620, 637, 948
FalI AAGNNNNNCTT 2 cut(s) 831, 863
FatI CATG 3 cut(s) 616, 633, 944
FauNDI CATATG 1 cut(s) 244
FbaI TGATCA 1 cut(s) 660
FblI GTMKAC 2 cut(s) 727, 1041
Fnu4HI GCNGC 1 cut(s) 982
FokI GGATG 1 cut(s) 1044
Fsp4HI GCNGC 1 cut(s) 982
FspBI CTAG 2 cut(s) 413, 500
GluI GCNGC 1 cut(s) 982
GsuI CTGGAG 1 cut(s) 851
HaeIII GGCC 2 cut(s) 126, 1056
HapII CCGG 3 cut(s) 356, 731, 1053
Hin1II CATG 3 cut(s) 620, 637, 948
HinfI GANTC 5 cut(s) 173, 337, 605, 868, 1049
HpaII CCGG 3 cut(s) 356, 731, 1053
HphI GGTGA 4 cut(s) 394, 515, 575, 649
Hpy166II GTNNAC 4 cut(s) 385, 541, 728, 1042
Hpy188I TCNGA 4 cut(s) 141, 286, 532, 557
Hpy188III TCNNGA 3 cut(s) 63, 851, 1112
Hpy8I GTNNAC 4 cut(s) 385, 541, 728, 1042
HpyAV CCTTC 2 cut(s) 110, 265
HpyCH4IV ACGT 4 cut(s) 34, 521, 897, 1126
HpyCH4V TGCA 5 cut(s) 95, 136, 323, 392, 788
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 123, 320, 490, 941
HpyF3I CTNAG 1 cut(s) 1005
HpySE526I ACGT 4 cut(s) 34, 521, 897, 1126
Hsp92II CATG 3 cut(s) 620, 637, 948
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 2 cut(s) 8, 660
LmnI GCTCC 2 cut(s) 832, 1101
Lsp1109I GCAGC 1 cut(s) 968
LweI GCATC 2 cut(s) 145, 401
MaeI CTAG 2 cut(s) 413, 500
MaeII ACGT 4 cut(s) 34, 521, 897, 1126
MaeIII GTNAC 5 cut(s) 1000, 1019, 1027, 1117, 1127
MalI GATC 2 cut(s) 10, 662
MboI GATC 2 cut(s) 8, 660
MflI RGATCY 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 6 cut(s) 232, 589, 715, 874, 972, 1135
MluNI TGGCCA 1 cut(s) 126
MlyI GAGTC 1 cut(s) 614
MmeI TCCRAC 2 cut(s) 169, 420
MnlI CCTC 5 cut(s) 483, 677, 836, 980, 989
Mox20I TGGCCA 1 cut(s) 126
MscI TGGCCA 1 cut(s) 126
MseI TTAA 4 cut(s) 447, 702, 747, 965
MslI CAYNNNNRTG 1 cut(s) 615
Msp20I TGGCCA 1 cut(s) 126
MspI CCGG 3 cut(s) 356, 731, 1053
MspR9I CCNGG 5 cut(s) 83, 357, 732, 753, 830
MvaI CCWGG 3 cut(s) 83, 753, 830
MwoI GCNNNNNNNGC 5 cut(s) 92, 123, 320, 490, 941
NciI CCSGG 2 cut(s) 357, 732
NdeI CATATG 1 cut(s) 244
NdeII GATC 2 cut(s) 8, 660
NlaIII CATG 3 cut(s) 620, 637, 948
NlaIV GGNNCC 1 cut(s) 1103
NmuCI GTSAC 3 cut(s) 1000, 1019, 1117
NspI RCATGY 1 cut(s) 948
NspV TTCGAA 1 cut(s) 1088
PaeI GCATGC 1 cut(s) 948
PcsI WCGNNNNNNNCGW 1 cut(s) 894
PfeI GAWTC 4 cut(s) 173, 337, 868, 1049
PflFI GACNNNGTC 1 cut(s) 1037
PfoI TCCNGGA 1 cut(s) 828
PkrI GCNGC 1 cut(s) 983
PleI GAGTC 1 cut(s) 613
PpsI GAGTC 1 cut(s) 613
Ppu21I YACGTR 1 cut(s) 35
Psp6I CCWGG 3 cut(s) 81, 751, 828
PspGI CCWGG 3 cut(s) 81, 751, 828
PspN4I GGNNCC 1 cut(s) 1103
PspPI GGNCC 2 cut(s) 442, 959
PsuI RGATCY 1 cut(s) 8
PsyI GACNNNGTC 1 cut(s) 1037
RsaI GTAC 2 cut(s) 37, 813
RsaNI GTAC 2 cut(s) 36, 812
RseI CAYNNNNRTG 1 cut(s) 615
SaqAI TTAA 4 cut(s) 447, 702, 747, 965
SatI GCNGC 1 cut(s) 982
Sau3AI GATC 2 cut(s) 8, 660
Sau96I GGNCC 2 cut(s) 442, 959
ScaI AGTACT 1 cut(s) 813
SchI GAGTC 1 cut(s) 614
ScrFI CCNGG 5 cut(s) 83, 357, 732, 753, 830
SfaNI GCATC 2 cut(s) 145, 401
SfuI TTCGAA 1 cut(s) 1088
SinI GGWCC 2 cut(s) 442, 959
SmiMI CAYNNNNRTG 1 cut(s) 615
SmlI CTYRAG 1 cut(s) 736
SmoI CTYRAG 1 cut(s) 736
SnaBI TACGTA 1 cut(s) 35
SphI GCATGC 1 cut(s) 948
Sse9I AATT 6 cut(s) 232, 589, 715, 874, 972, 1135
SsiI CCGC 2 cut(s) 561, 984
SspMI CTAG 2 cut(s) 413, 500
StyD4I CCNGG 5 cut(s) 81, 355, 730, 751, 828
TaiI ACGT 4 cut(s) 37, 524, 900, 1129
TaqI TCGA 4 cut(s) 363, 603, 866, 1088
TasI AATT 6 cut(s) 232, 589, 715, 874, 972, 1135
TatI WGTACW 1 cut(s) 811
TfiI GAWTC 4 cut(s) 173, 337, 868, 1049
Tru1I TTAA 4 cut(s) 447, 702, 747, 965
Tru9I TTAA 4 cut(s) 447, 702, 747, 965
TseFI GTSAC 3 cut(s) 1000, 1019, 1117
TseI GCWGC 1 cut(s) 981
Tsp45I GTSAC 3 cut(s) 1000, 1019, 1117
TspDTI ATGAA 6 cut(s) 151, 571, 633, 789, 847, 893
Tth111I GACNNNGTC 1 cut(s) 1037
VpaK11BI GGWCC 2 cut(s) 442, 959
XapI RAATTY 4 cut(s) 232, 589, 715, 874
XceI RCATGY 1 cut(s) 948
XmiI GTMKAC 2 cut(s) 727, 1041
XspI CTAG 2 cut(s) 413, 500
ZrmI AGTACT 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.