Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003687

Physical Location & Seq

Genomic Coordinates Reverse (-)

21875825 .. 21890198

Gene Structure 14374 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1105600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3012 bp

ATGGCCAATAACCCTCAATTTTCAGGTCTACAGCCTCTTCGGCCTCCTATGGTTGGTGCTGTTGATCCTCCTCGAAGTCTGCCACCCATGTCTTTTCAATTCCGCCCAGTAGTTCCGACACCACATTCGCAGGAGTTCATTCCTGTGGCTTCTCAAAATTTTCAGCATGCCGGCAGGGCTCCATTGATGAATGTTAGGCTGCCTCCCCCAAATCACCAACCCCAGTTTTCACAACCAATGCAGCATCTACCTCCAAGGTCTGGTCAGCCAGGTCATGGTATGCCTCCATCACAGGCAATTCCATTGCCCGTTGCTCAGCCTAACAGGAATTTTGCATCTGAGTTGCCTCTACCTCAGCCTAATTCACAAGCTCCAAATAACATCATGCCTAACTTTGGTGGCCCACGAGCAGCTGTCCCTTCATCATATACTTTTGCAACTTCTTCTTATGGTCAAATACCAAGAAGTTTCAATGACTCCACCCAGTATCAGTCTATATCTCAATCGCATGCAACCAGTGTTTCATCCGAGGGACAAGTTACACCTCTGCAGCAAGCCAGTGAACGGCCTGCTGTAACCACCTCCATTGCTCCAGAAACAGGTGTTCGACCTCCCCCGGCCGGAGGGACTGCAGCAGAGTGGAGAGAGCATACATCTGCTGATGGAAGAAAATTTTATTACAACAGGAGGACAGCATTATCTACTTGGGAGAAGCCTTTGGAACTGATGACACCAATGGAGAGGGCAGATGCATCAACAAACTGGAAGGAGTTTACCAGTCCTGATGGAAGAAAGTATTACTACAACAAGGTCACCAAAGAATCAAGATGGACTATGCCTGAGGAACTTAAGTTGGCTCGAGAGCTAGTAGAAAAGGCGTCTGTGAAGGAAATACAACAGGAAACGATTGGTGATCATCATGCCACAGTCACTGTTTCTCCCTCTGTAGCTGAAGCTGATGCTTCAATCGGTACTGCTCAAGTAAAATCAAGCCCTGTTTTGGTGGCACCAGTTGTTGCCACTGGTGATGTTCAAACTGCTGTACCATCGAGAACATCTGCCTCACCTGTTGTGGATTCTCCCTTGAAAGAAAATCCCAGTGAAGTTCAGACACCTGTTGCACCATCTGCCTCTGTTATGGGAAGTACTGAAGCTGCTGTCACAATCAACGATACTGGTGCAGAACCAATGGAAATATCAGCTCAGGATTTTGATAGTTCTGCTGATGGAGTTCCTGCTCAGGAGAATGAGGAAGCTAAGGATGATGCAACGGGAAAGAAGATTAATGATATTGCCGCAGAAGAAAAATCAGTTGATCGGGAACCCATACCATATGAAAATAAACTGGAGGCCAAAAATGCATTTAAAGCGCTCCTTGAGTCTACAAATATTGGTTCTGATTGGACTTGGGATCGGGCCATGAGAGTGATAATTAATGACAAGAGATATGGTGCTCTCAAAACGCTTGGAGAGCGGAAGCAAGCTTTTAATGAGTTCTTGGTACAAAGGAAAAAGCAGGAGGCTGAGGAGAGGCGTATCAAACAGAAAAAGGCACGGGATGAATTCAAAACAATGCTGGAAGAATGCTCAGAGTTAACATCATCTGCAAGATGGGGAAAAGTAGAGAGCATATTCGAAAATGATGAACGTTTCAAAGCTGTTGAACGGGATAGAGATCGCAGGGAGATGTTTGAAGACTATATTGAGGAACTCCAAAAGAAAGAACGAGCAAAAGCACAGGAGGAACGTAAGCGCAATGTATTGGAATACAGGAGATTCTTGGAATCTTGTGATTTCATCAAGGCAAACATCCAGTGGAGAAAAGTTCAAGATCGTTTAGAGGCTGATGAAAGATGTTCACGTCTTGAAAAAATTGATCGTTTGGAAATTTTCCAGGAATATTTGCTGGATCTACAAAAGGAAGAGGAGGAGCAGAAGAGGATACAGAAGGAAGAATTGAGAAAGGCAGAACGTAAAAACCGTGATGAGTTCCGCAAATTAATGGAAGAACATCTCACGGAAGGCACTCTTACATTCAAAACTCAGTGGCGTGATTACTGCATGAAGGTTAAAGACCTACCTGCATATCTGGCAGTTGCATCAAACGCCTCCGGTGCTACTCCAAAAGATTTGTTCGATGATGTTGTTGAGGAACTAACGAAACAATATCAGGAGGACAAAACTCGAATAAAAGATGCCGTGAAATTGGGAAAGGTCGTGCTTGCATCAACTTGGAAGTTTGAAGACTTCAAAACTGTTTTATCAAATGATATTGGCTCTCCACCGATATCAGATGCCAACTTAAAGCTAGTTTTCAATGAGTTACTGGAACGAGTGAGGGAAAAGGAGGAGAAAGAAGCTAAAAAGCTTAAACGTCTTGCAGAGGACTTCTCTCATCTTTTATCTTCTATCAAGGAGATAACTCCATCTTCTAAGTGGGAGGACTACAAACCACTTCTTGAAATTAGTCAAGAGTACAGTGGCATTGGCGACGAGAACTTCTGCAAGGAGATATTTGAAAAGCATGTAATGCAACTGAAAGAACAAGCAAAAGAGGAGAGAAAAAGAAAGGAGGAAAAGGCAAGGAAGGAAAAGGAGAGAGAAGAAAGAGACAAAAGAAGGGCGAAGCCAAGAAGGGAAAAAGAGGTGGAATATGAACGAGAAAACGGGGAACACAACAAGAAAGATGGAGCAGACAATGAAATAGCTGATGCAATGGATATTCATGAGTCCAAAGAGAAAAAGAGGTCAGGACATGACGATAATAGGAGACATAGGAAGCGGCATCATAGTGATGAAGATCATGGGAATGGAAATGAGAATGATCGGTCTAGAAAATCTCACAGATCTAGCAGTGACCACAAGAAATCAAGACGGGAACCTGGTCCTGAATCAGATTCCGAAAGCAGACATAGAAGACATAAAAGAGATCACCGGAATGGTTCTCGTAGAAGTGGTGATCGTGAGGATCTAGAAGATGGGGAATTTGGCAATGTTGGGGAAAGTCGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005685 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006928 GO:0006996 GO:0007010 GO:0008150 GO:0008152 GO:0008360 GO:0008380 GO:0009987 GO:0010467 GO:0010564 GO:0016043 GO:0016070 GO:0016071 GO:0016363 GO:0016477 GO:0016604 GO:0016607 GO:0022603 GO:0022604 GO:0030532 GO:0031974 GO:0031981 GO:0032465 GO:0032991 GO:0034399 GO:0034641 GO:0040011 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051302 GO:0051674 GO:0051726 GO:0065007 GO:0065008 GO:0070013 GO:0070064 GO:0071004 GO:0071010 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097525 GO:0120114 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

FF WW

Protein Analysis

1004

Amino Acids

114.7

Weight (kDa)

6.4

Isoelectric Point (pI)

56.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	213 - 239	6e-08	WW domain
WW	PF00397	254 - 280	3.3e-09	WW domain
FF	PF01846	450 - 499	9.7e-16	FF domain
FF	PF01846	517 - 567	3.4e-15	FF domain
FF_PRPF40A	PF25432	722 - 781	2.6e-22	PRPF40A-like, FF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003687)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g03330 FvH4_2g03330 FvH4_2g03330 FvH4_2g03330 FvH4_2g03330 FvH4_2g03330 FvH4_2g03330 FvH4_2g03361 FvH4_2g03361
malus_domestica	MD05G1105600.v1.1
prunus_persica	Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1
pyrus_communis	pycom05g10260
rosa_chinensis	RchiOBHm_Chr6g0248471
rosa_laevigata	RLG00000015152
rosa_multiflora	Rmu_sc0002553.1_g000012
rosa_roxburghii	Rroxscaffold_7G00213270
rosa_rugosa	Rorug05G0531500
rosa_samantha	Rh6AG047400 Rh6BG043700 Rh6CG040400 Rh6DG036900
rosa_wichuraiana	Rw6G004110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2089
AccB1I	GGYRCC	1 cut(s)	1006
AccB7I	CCANNNNNTGG	2 cut(s)	260, 275
AccBSI	CCGCTC	1 cut(s)	1474
AccI	GTMKAC	2 cut(s)	28, 1382
AciI	CCGC	5 cut(s)	103, 1296, 1474, 1993, 2782
AclI	AACGTT	1 cut(s)	1648
AclWI	GGATC	4 cut(s)	59, 1419, 1917, 2976
AcoI	YGGCCR	2 cut(s)	3, 618
AcsI	RAATTY	6 cut(s)	157, 328, 671, 1562, 1887, 2984
AcuI	CTGAAG	2 cut(s)	972, 1170
AcyI	GRCGYC	1 cut(s)	878
AfaI	GTAC	5 cut(s)	973, 1044, 1147, 1503, 2477
AfeI	AGCGCT	1 cut(s)	1371
AfiI	CCNNNNNNNGG	9 cut(s)	53, 260, 275, 395, 564, 599, 620, 623, 1000
AflII	CTTAAG	1 cut(s)	848
AjiI	CACGTC	1 cut(s)	1862
AjnI	CCWGG	3 cut(s)	268, 1893, 2881
Alw21I	GWGCWC	1 cut(s)	1456
Alw26I	GTCTC	2 cut(s)	2604, 2764
AlwI	GGATC	4 cut(s)	59, 1419, 1917, 2976
AlwNI	CAGNNNCTG	1 cut(s)	932
Ama87I	CYCGRG	1 cut(s)	858
Aor51HI	AGCGCT	1 cut(s)	1371
AoxI	GGCC	7 cut(s)	3, 41, 400, 566, 618, 1350, 1416
ApeKI	GCWGC	6 cut(s)	199, 241, 410, 550, 632, 1154
ApoI	RAATTY	6 cut(s)	157, 328, 671, 1562, 1887, 2984
ArsI	GACNNNNNNTTYG	2 cut(s)	109, 141
AseI	ATTAAT	3 cut(s)	1284, 1434, 2000
AspLEI	GCGC	2 cut(s)	1372, 1755
AspS9I	GGNCC	3 cut(s)	401, 1416, 2885
AsuC2I	CCSGG	1 cut(s)	617
AsuHPI	GGTGA	7 cut(s)	206, 805, 923, 1037, 1056, 2924, 2969
AsuII	TTCGAA	1 cut(s)	1635
AvaI	CYCGRG	1 cut(s)	858
AvaII	GGWCC	1 cut(s)	2885
AxyI	CCTNAGG	1 cut(s)	840
BaeI	ACNNNNGTAYC	2 cut(s)	1026, 1059
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	1006
BanII	GRGCYC	1 cut(s)	181
BauI	CACGAG	1 cut(s)	405
BbsI	GAAGAC	3 cut(s)	1701, 2250, 2923
Bbv12I	GWGCWC	1 cut(s)	1456
BbvCI	CCTCAGC	2 cut(s)	354, 1524
BbvI	GCAGC	6 cut(s)	186, 253, 422, 562, 644, 1141
BceAI	ACGGC	2 cut(s)	581, 2183
BcgI	CGANNNNNNTGC	2 cut(s)	1160, 1194
BciT130I	CCWGG	3 cut(s)	270, 1895, 2883
BciVI	GTATCC	1 cut(s)	1935
BclI	TGATCA	1 cut(s)	913
BcnI	CCSGG	1 cut(s)	617
BcoDI	GTCTC	2 cut(s)	2604, 2764
BfaI	CTAG	5 cut(s)	866, 2309, 2832, 2850, 2972
BfmI	CTRYAG	4 cut(s)	29, 548, 630, 945
BfoI	RGCGCY	1 cut(s)	1373
BfrI	CTTAAG	1 cut(s)	848
BfuAI	ACCTGC	1 cut(s)	2089
BfuI	GTATCC	1 cut(s)	1935
BglI	GCCNNNNNGGC	2 cut(s)	40, 176
BglII	AGATCT	1 cut(s)	2846
BisI	GCNGC	8 cut(s)	200, 242, 411, 551, 633, 1155, 1296, 2783
BlpI	GCTNAGC	1 cut(s)	315
BlsI	GCNGC	8 cut(s)	201, 243, 412, 552, 634, 1156, 1297, 2784
BmcAI	AGTACT	1 cut(s)	1147
Bme1390I	CCNGG	4 cut(s)	270, 617, 1895, 2883
Bme18I	GGWCC	1 cut(s)	2885
BmeT110I	CYCGRG	1 cut(s)	858
BmgBI	CACGTC	1 cut(s)	1862
BmgT120I	GGNCC	3 cut(s)	401, 1416, 2885
BmiI	GGNNCC	4 cut(s)	180, 1008, 1323, 2880
BmrFI	CCNGG	4 cut(s)	270, 617, 1895, 2883
BmrI	ACTGGG	4 cut(s)	101, 217, 478, 1092
BmuI	ACTGGG	4 cut(s)	101, 217, 478, 1092
BpiI	GAAGAC	3 cut(s)	1701, 2250, 2923
BplI	GAGNNNNNCTC	2 cut(s)	2375, 2407
BpmI	CTGGAG	2 cut(s)	576, 1367
Bpu10I	CCTNAGC	5 cut(s)	354, 1203, 1239, 1257, 1524
Bpu1102I	GCTNAGC	1 cut(s)	315
Bpu14I	TTCGAA	1 cut(s)	1635
BpuEI	CTTGAG	2 cut(s)	963, 1397
BpuMI	CCSGG	1 cut(s)	617
BsaBI	GATNNNNATC	2 cut(s)	1674, 2799
BsaHI	GRCGYC	1 cut(s)	878
BsaJI	CCNNGG	3 cut(s)	254, 528, 615
BsaWI	WCCGGW	2 cut(s)	2111, 2934
BsaXI	ACNNNNNCTCC	2 cut(s)	922, 952
Bsc4I	CCNNNNNNNGG	9 cut(s)	53, 260, 275, 395, 564, 599, 620, 623, 1000
Bse118I	RCCGGY	1 cut(s)	170
Bse21I	CCTNAGG	1 cut(s)	840
Bse3DI	GCAATG	5 cut(s)	302, 585, 1762, 2721, 2998
Bse8I	GATNNNNATC	2 cut(s)	1674, 2799
BseBI	CCWGG	3 cut(s)	270, 1895, 2883
BseDI	CCNNGG	3 cut(s)	254, 528, 615
BseGI	GGATG	4 cut(s)	524, 1267, 1564, 1809
BseJI	GATNNNNATC	2 cut(s)	1674, 2799
BseLI	CCNNNNNNNGG	9 cut(s)	53, 260, 275, 395, 564, 599, 620, 623, 1000
BseMI	GCAATG	5 cut(s)	302, 585, 1762, 2721, 2998
BseMII	CTCAG	9 cut(s)	329, 330, 368, 831, 1217, 1253, 1515, 1602, 2057
BseRI	GAGGAG	6 cut(s)	60, 1541, 1940, 1943, 2363, 2570
BseX3I	CGGCCG	1 cut(s)	618
BseXI	GCAGC	6 cut(s)	186, 253, 422, 562, 644, 1141
BsgI	GTGCAG	1 cut(s)	1200
Bsh1285I	CGRYCG	1 cut(s)	621
BshFI	GGCC	7 cut(s)	5, 43, 402, 568, 620, 1352, 1418
BshNI	GGYRCC	1 cut(s)	1006
BsiEI	CGRYCG	1 cut(s)	621
BsiHKAI	GWGCWC	1 cut(s)	1456
BsiHKCI	CYCGRG	1 cut(s)	858
BsiSI	CCGG	5 cut(s)	171, 617, 621, 2112, 2935
BslFI	GGGAC	3 cut(s)	401, 546, 640
BslI	CCNNNNNNNGG	9 cut(s)	53, 260, 275, 395, 564, 599, 620, 623, 1000
BsmAI	GTCTC	2 cut(s)	2604, 2764
BsmFI	GGGAC	3 cut(s)	401, 546, 640
BsmI	GAATGC	1 cut(s)	1589
BsnI	GGCC	7 cut(s)	5, 43, 402, 568, 620, 1352, 1418
BsoBI	CYCGRG	1 cut(s)	858
Bsp119I	TTCGAA	1 cut(s)	1635
Bsp1286I	GDGCHC	2 cut(s)	181, 1456
Bsp1720I	GCTNAGC	1 cut(s)	315
BspACI	CCGC	5 cut(s)	103, 1296, 1474, 1993, 2782
BspANI	GGCC	7 cut(s)	5, 43, 402, 568, 620, 1352, 1418
BspCNI	CTCAG	9 cut(s)	328, 331, 367, 832, 1216, 1252, 1516, 1601, 2056
BspHI	TCATGA	1 cut(s)	2725
BspLI	GGNNCC	4 cut(s)	180, 1008, 1323, 2880
BspMAI	CTGCAG	2 cut(s)	552, 634
BspMI	ACCTGC	1 cut(s)	2089
BspPI	GGATC	4 cut(s)	59, 1419, 1917, 2976
BspT104I	TTCGAA	1 cut(s)	1635
BspT107I	GGYRCC	1 cut(s)	1006
BspTI	CTTAAG	1 cut(s)	848
BsrBI	CCGCTC	1 cut(s)	1474
BsrDI	GCAATG	5 cut(s)	302, 585, 1762, 2721, 2998
BsrFI	RCCGGY	1 cut(s)	170
BssAI	RCCGGY	1 cut(s)	170
BssECI	CCNNGG	3 cut(s)	254, 528, 615
BssNI	GRCGYC	1 cut(s)	878
BssSI	CACGAG	1 cut(s)	405
BssT1I	CCWWGG	1 cut(s)	254
Bst2BI	CACGAG	1 cut(s)	405
Bst2UI	CCWGG	3 cut(s)	270, 1895, 2883
Bst4CI	ACNGT	5 cut(s)	928, 934, 1982, 2257, 2480
Bst6I	CTCTTC	3 cut(s)	42, 1917, 1931
BstACI	GRCGYC	1 cut(s)	878
BstAFI	CTTAAG	1 cut(s)	848
BstAPI	GCANNNNNTGC	2 cut(s)	1127, 2530
BstBI	TTCGAA	1 cut(s)	1635
BstC8I	GCNNGC	7 cut(s)	168, 172, 510, 555, 570, 1482, 2223
BstEII	GGTNACC	1 cut(s)	811
BstF5I	GGATG	4 cut(s)	524, 1267, 1564, 1809
BstH2I	RGCGCY	1 cut(s)	1373
BstHHI	GCGC	2 cut(s)	1372, 1755
BstMAI	GTCTC	2 cut(s)	2604, 2764
BstMCI	CGRYCG	1 cut(s)	621
BstNI	CCWGG	3 cut(s)	270, 1895, 2883
BstNSI	RCATGY	3 cut(s)	170, 512, 2528
BstPI	GGTNACC	1 cut(s)	811
BstSCI	CCNGG	4 cut(s)	268, 615, 1893, 2881
BstSFI	CTRYAG	4 cut(s)	29, 548, 630, 945
BstV1I	GCAGC	6 cut(s)	186, 253, 422, 562, 644, 1141
BstV2I	GAAGAC	3 cut(s)	1701, 2250, 2923
BstX2I	RGATCY	3 cut(s)	1909, 2846, 2968
BstYI	RGATCY	3 cut(s)	1909, 2846, 2968
BstZI	CGGCCG	1 cut(s)	618
Bsu36I	CCTNAGG	1 cut(s)	840
BsuI	GTATCC	1 cut(s)	1935
BsuRI	GGCC	7 cut(s)	5, 43, 402, 568, 620, 1352, 1418
BtrI	CACGTC	1 cut(s)	1862
BtsCI	GGATG	4 cut(s)	524, 1267, 1564, 1809
BtsI	GCAGTG	1 cut(s)	2860
BtsIMutI	CAGTG	9 cut(s)	523, 565, 930, 1020, 1105, 1820, 2051, 2485, 2860
BveI	ACCTGC	1 cut(s)	2089
Cac8I	GCNNGC	7 cut(s)	168, 172, 510, 555, 570, 1482, 2223
CaiI	CAGNNNCTG	1 cut(s)	932
CciI	TCATGA	1 cut(s)	2725
CfoI	GCGC	2 cut(s)	1372, 1755
Cfr10I	RCCGGY	1 cut(s)	170
Cfr13I	GGNCC	3 cut(s)	401, 1416, 2885
CseI	GACGC	1 cut(s)	867
CsiI	ACCWGGT	1 cut(s)	2881
Csp6I	GTAC	5 cut(s)	972, 1043, 1146, 1502, 2476
CspCI	CAANNNNNGTGG	2 cut(s)	568, 603
CviQI	GTAC	5 cut(s)	972, 1043, 1146, 1502, 2476
DraI	TTTAAA	1 cut(s)	1366
EaeI	YGGCCR	2 cut(s)	3, 618
EagI	CGGCCG	1 cut(s)	618
Eam1104I	CTCTTC	3 cut(s)	42, 1917, 1931
EarI	CTCTTC	3 cut(s)	42, 1917, 1931
EciI	GGCGGA	1 cut(s)	92
EclXI	CGGCCG	1 cut(s)	618
Eco130I	CCWWGG	1 cut(s)	254
Eco24I	GRGCYC	1 cut(s)	181
Eco32I	GATATC	1 cut(s)	2289
Eco47I	GGWCC	1 cut(s)	2885
Eco47III	AGCGCT	1 cut(s)	1371
Eco52I	CGGCCG	1 cut(s)	618
Eco57I	CTGAAG	2 cut(s)	972, 1170
Eco81I	CCTNAGG	1 cut(s)	840
Eco88I	CYCGRG	1 cut(s)	858
Eco91I	GGTNACC	1 cut(s)	811
EcoO65I	GGTNACC	1 cut(s)	811
EcoRI	GAATTC	1 cut(s)	1562
EcoRII	CCWGG	3 cut(s)	268, 1893, 2881
EcoRV	GATATC	1 cut(s)	2289
EcoT14I	CCWWGG	1 cut(s)	254
EcoT22I	ATGCAT	2 cut(s)	754, 1363
EcoT38I	GRGCYC	1 cut(s)	181
ErhI	CCWWGG	1 cut(s)	254
FalI	AAGNNNNNCTT	4 cut(s)	1359, 1391, 2013, 2045
FaqI	GGGAC	3 cut(s)	401, 546, 640
FauNDI	CATATG	1 cut(s)	1333
FbaI	TGATCA	1 cut(s)	913
FblI	GTMKAC	2 cut(s)	28, 1382
Fnu4HI	GCNGC	8 cut(s)	200, 242, 411, 551, 633, 1155, 1296, 2783
FokI	GGATG	4 cut(s)	511, 1274, 1571, 1796
FriOI	GRGCYC	1 cut(s)	181
Fsp4HI	GCNGC	8 cut(s)	200, 242, 411, 551, 633, 1155, 1296, 2783
FspBI	CTAG	5 cut(s)	866, 2309, 2832, 2850, 2972
GlaI	GCGC	2 cut(s)	1371, 1754
GluI	GCNGC	8 cut(s)	200, 242, 411, 551, 633, 1155, 1296, 2783
GsuI	CTGGAG	2 cut(s)	576, 1367
HaeII	RGCGCY	1 cut(s)	1373
HaeIII	GGCC	7 cut(s)	5, 43, 402, 568, 620, 1352, 1418
HapII	CCGG	5 cut(s)	171, 617, 621, 2112, 2935
HgaI	GACGC	1 cut(s)	867
HhaI	GCGC	2 cut(s)	1372, 1755
Hin1I	GRCGYC	1 cut(s)	878
Hin6I	GCGC	2 cut(s)	1370, 1753
HinP1I	GCGC	2 cut(s)	1370, 1753
HincII	GTYRAC	1 cut(s)	1596
HindII	GTYRAC	1 cut(s)	1596
HindIII	AAGCTT	2 cut(s)	1482, 2366
HinfI	GANTC	9 cut(s)	476, 821, 1076, 1379, 1776, 1784, 2729, 2891, 2897
HpaI	GTTAAC	1 cut(s)	1596
HpaII	CCGG	5 cut(s)	171, 617, 621, 2112, 2935
HphI	GGTGA	7 cut(s)	206, 805, 923, 1037, 1056, 2924, 2969
Hpy166II	GTNNAC	6 cut(s)	29, 563, 774, 1383, 1596, 1859
Hpy188I	TCNGA	9 cut(s)	117, 340, 529, 1110, 1399, 1591, 2293, 2896, 2902
Hpy8I	GTNNAC	6 cut(s)	29, 563, 774, 1383, 1596, 1859
Hpy99I	CGWCG	1 cut(s)	2495
HpyAV	CCTTC	9 cut(s)	429, 760, 880, 1942, 2015, 2059, 2581, 2613, 2628
HpyCH4III	ACNGT	5 cut(s)	928, 934, 1982, 2257, 2480
HpyCH4IV	ACGT	5 cut(s)	1648, 1747, 1861, 1972, 2374
HpySE526I	ACGT	5 cut(s)	1648, 1747, 1861, 1972, 2374
Hsp92I	GRCGYC	1 cut(s)	878
HspAI	GCGC	2 cut(s)	1370, 1753
KroI	GCCGGC	1 cut(s)	170
KroNI	GCCGGC	1 cut(s)	172
Ksp22I	TGATCA	1 cut(s)	913
KspAI	GTTAAC	1 cut(s)	1596
LmnI	GCTCC	6 cut(s)	184, 376, 595, 1377, 1930, 2690
Lsp1109I	GCAGC	6 cut(s)	186, 253, 422, 562, 644, 1141
MabI	ACCWGGT	1 cut(s)	2881
MaeI	CTAG	5 cut(s)	866, 2309, 2832, 2850, 2972
MaeII	ACGT	5 cut(s)	1648, 1747, 1861, 1972, 2374
MaeIII	GTNAC	7 cut(s)	538, 574, 811, 928, 1159, 2322, 2855
MbiI	CCGCTC	1 cut(s)	1474
MflI	RGATCY	3 cut(s)	1909, 2846, 2968
MhlI	GDGCHC	2 cut(s)	181, 1456
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	470, 1388, 2738
MmeI	TCCRAC	1 cut(s)	140
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	754, 1363
MroNI	GCCGGC	1 cut(s)	170
MscI	TGGCCA	1 cut(s)	5
MslI	CAYNNNNRTG	6 cut(s)	143, 1424, 2790, 2793, 2808, 2937
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	413
MspCI	CTTAAG	1 cut(s)	848
MspI	CCGG	5 cut(s)	171, 617, 621, 2112, 2935
MspR9I	CCNGG	4 cut(s)	270, 617, 1895, 2883
Mva1269I	GAATGC	1 cut(s)	1589
MvaI	CCWGG	3 cut(s)	270, 1895, 2883
NaeI	GCCGGC	1 cut(s)	172
NciI	CCSGG	1 cut(s)	617
NdeI	CATATG	1 cut(s)	1333
NgoMIV	GCCGGC	1 cut(s)	170
NlaIV	GGNNCC	4 cut(s)	180, 1008, 1323, 2880
NmuCI	GTSAC	4 cut(s)	811, 928, 1159, 2855
NsiI	ATGCAT	2 cut(s)	754, 1363
NspI	RCATGY	3 cut(s)	170, 512, 2528
NspV	TTCGAA	1 cut(s)	1635
PaeI	GCATGC	2 cut(s)	170, 512
PaeR7I	CTCGAG	1 cut(s)	858
PagI	TCATGA	1 cut(s)	2725
PcsI	WCGNNNNNNNCGW	1 cut(s)	1978
PctI	GAATGC	1 cut(s)	1589
PdiI	GCCGGC	1 cut(s)	172
PfeI	GAWTC	6 cut(s)	821, 1076, 1776, 1784, 2891, 2897
PflMI	CCANNNNNTGG	2 cut(s)	260, 275
PfoI	TCCNGGA	1 cut(s)	1893
PkrI	GCNGC	8 cut(s)	201, 243, 412, 552, 634, 1156, 1297, 2784
PleI	GAGTC	3 cut(s)	470, 1387, 2737
PpsI	GAGTC	3 cut(s)	470, 1387, 2737
PshBI	ATTAAT	3 cut(s)	1284, 1434, 2000
Psp1406I	AACGTT	1 cut(s)	1648
Psp6I	CCWGG	3 cut(s)	268, 1893, 2881
PspEI	GGTNACC	1 cut(s)	811
PspGI	CCWGG	3 cut(s)	268, 1893, 2881
PspN4I	GGNNCC	4 cut(s)	180, 1008, 1323, 2880
PspPI	GGNCC	3 cut(s)	401, 1416, 2885
PstI	CTGCAG	2 cut(s)	552, 634
PstNI	CAGNNNCTG	1 cut(s)	932
PsuI	RGATCY	3 cut(s)	1909, 2846, 2968
PvuII	CAGCTG	1 cut(s)	413
RsaI	GTAC	5 cut(s)	973, 1044, 1147, 1503, 2477
RsaNI	GTAC	5 cut(s)	972, 1043, 1146, 1502, 2476
RseI	CAYNNNNRTG	6 cut(s)	143, 1424, 2790, 2793, 2808, 2937
SatI	GCNGC	8 cut(s)	200, 242, 411, 551, 633, 1155, 1296, 2783
Sau96I	GGNCC	3 cut(s)	401, 1416, 2885
ScaI	AGTACT	1 cut(s)	1147
SchI	GAGTC	3 cut(s)	470, 1388, 2738
ScrFI	CCNGG	4 cut(s)	270, 617, 1895, 2883
SduI	GDGCHC	2 cut(s)	181, 1456
SexAI	ACCWGGT	1 cut(s)	2881
SfcI	CTRYAG	4 cut(s)	29, 548, 630, 945
Sfr274I	CTCGAG	1 cut(s)	858
SfuI	TTCGAA	1 cut(s)	1635
SinI	GGWCC	1 cut(s)	2885
SlaI	CTCGAG	1 cut(s)	858
SmiMI	CAYNNNNRTG	6 cut(s)	143, 1424, 2790, 2793, 2808, 2937
SmlI	CTYRAG	4 cut(s)	848, 858, 978, 1376
SmoI	CTYRAG	4 cut(s)	848, 858, 978, 1376
SphI	GCATGC	2 cut(s)	170, 512
SsiI	CCGC	5 cut(s)	103, 1296, 1474, 1993, 2782
SspI	AATATT	2 cut(s)	1390, 1901
SspMI	CTAG	5 cut(s)	866, 2309, 2832, 2850, 2972
StyD4I	CCNGG	4 cut(s)	268, 615, 1893, 2881
StyI	CCWWGG	1 cut(s)	254
TaaI	ACNGT	5 cut(s)	928, 934, 1982, 2257, 2480
TaiI	ACGT	5 cut(s)	1651, 1750, 1864, 1975, 2377
TaqI	TCGA	7 cut(s)	73, 607, 859, 1049, 1635, 2136, 2185
TaqII	GACCGA	1 cut(s)	2817
TatI	WGTACW	2 cut(s)	1145, 2475
TauI	GCSGC	2 cut(s)	1298, 2785
TfiI	GAWTC	6 cut(s)	821, 1076, 1776, 1784, 2891, 2897
TscAI	CASTG	9 cut(s)	523, 565, 937, 1027, 1105, 1820, 2051, 2485, 2860
TseFI	GTSAC	4 cut(s)	811, 928, 1159, 2855
TseI	GCWGC	6 cut(s)	199, 241, 410, 550, 632, 1154
Tsp45I	GTSAC	4 cut(s)	811, 928, 1159, 2855
TspGWI	ACGGA	1 cut(s)	2033
TspRI	CASTG	9 cut(s)	523, 565, 937, 1027, 1105, 1820, 2051, 2485, 2860
Van91I	CCANNNNNTGG	2 cut(s)	260, 275
Vha464I	CTTAAG	1 cut(s)	848
VpaK11BI	GGWCC	1 cut(s)	2885
VspI	ATTAAT	3 cut(s)	1284, 1434, 2000
XapI	RAATTY	6 cut(s)	157, 328, 671, 1562, 1887, 2984
XbaI	TCTAGA	2 cut(s)	2831, 2971
XceI	RCATGY	3 cut(s)	170, 512, 2528
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2433
XhoI	CTCGAG	1 cut(s)	858
XmiI	GTMKAC	2 cut(s)	28, 1382
XspI	CTAG	5 cut(s)	866, 2309, 2832, 2850, 2972
ZrmI	AGTACT	1 cut(s)	1147
Zsp2I	ATGCAT	2 cut(s)	754, 1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.