Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003687

Physical Location & Seq

Genomic Coordinates Forward (+)

4489301 .. 4504058

Gene Structure 14758 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ22271

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3069 bp

ATGGCCAATAATCCTCAATATTCAGGGTTGCAGCCTCTTCGGCCTCCTATGGTTGGTCCTGTGGATGCTCCTCGAAGTATGCCGCCCATGGGTTTTCAATTCCGGCCAGTAGTTCCAGCACCTCATTCGCAGGAGTTCATTCCTGTGGCTTCTCAAAATTTTCAGCATGTCGGAAGAGGTGTTCCACCAATGAATGTTAGACTGCCTCCTGCAATTCACCAACCTCAGTTTCCGCAACCAGTGCAGCAGTTACCTCCAAGATCTGGTCCACCAGGCCATGGTATGCTGCCACAGGCAATTCCCTTACCAGTTGGTCAGCCCATCAGGAACTTTGCACCTGAATTGCCTGTACCTCAGCCTAATGTGCTACCTCCAAATAATGTCATGTCTAACTTAAGTGCCCCACGGCCACCTCTTTCTTCATCATATACTTTTGCAACTTCTTCCTATGGTCAAATTCCAAGAAGTTTTAATGACTCGAACCAGTATCAGTCTATATCTCAATTGCATGCACCAAATGTTTCATCCGAGGGACAAGTTAGGTTATCATCTGATAGCCAGTCTGCTGTTGCTTCTGTACCTTTGCAGCCCACTGGTGAACGACCTTCTGTCACCACTTCAGTTGCTCCAGAAGCCAGCATCCAACCTATTTCTCTTGGAGGGAATCCAGCACAGTGGATTGACCATAAATCTGCTGATGGAAGAAGGTTTTATTACAACAGGAGGACACGATTATCTACTTGGGAGAAGCCTTTTGAATTGATGACACCACTGGAGAGGGCAGATGCATCAACTAACTGGAAGGAATTTACCAGTCCTGATGGAAAAAAGTATTTCCACAACAAGGTCACTAAAGAATCAAGATGGACTATACCTGAGGAACTGAAGTTGGCCCGGCAACAAGTGGAAAAGGCATCGGTCAAGGACATTCCTCAGGAGATGCCTGTAAATCATCACACTACAGTATCAGTTTCTCCCCCCGTAAGTGAAGCTCATACATCAACCACTACTGCTCAAGTAGCATCAAGCCCTGTTTCGGTGGCACCTGTTGTTGCCACTAGTGTTGTTCAAACTTCCACAGTTTCAAGATCATCTGTCTCACCTGTTGTGGCTTCTCCTGTAAAAGAAAATGCTGATGGAGTTCAGATACCTGCTGTTACACCATCTTCTGATGCTTTTGAAAATGCTGAAGCTGCTGTCACAGTCAACAATACTGCTGCAGAACTAATGGAAGATCCAAAGAATTTACCAACTCAGGAATTTGTTAGTTCTTCCGAGGAAGTTCATGTACAGGAGAATAAGGAACCTACAAATGATGTGACAGGAGAGAAAATTAATGACATTGCATCAGAAGAAAAACCAATTGATCCAGAACCCATAAGTTATTCAAATAAGCTGGAAGCTAAAGATGCATTTAAAGCACTCTTGGAGTCTACCAACATTGGATCTGATTGGACTTGGGATCGGGCCATGAGAGTGATAATTAATGACAAAAGATATGGTGCTTTGAAAACACTTGGAGAGCGGAAGCAAGCTTTTAATGAGTTTGTGGCCCAAAGGAAAAAACAAGAGGTTGAGGAGAGGCGGATCAAGCAGAAAAAAGCACGTGAAGATTTTACAAAGATGTTGGAAGAATGCTCAGAGTTAACATCATCCACAAGATGGGGCAAATTAGAGAGCATATTTGAAAATGATGAACGTTTCAAAGCAGTTGAAAGAGAGAGAGACCGCAGGGATTTGTTTGATAGCTATTTGGAGGAACTTCAGAAGAAAGAAAGAGCAAAGGCACAGGAGGAACGTAAGCTCCATATAGTGGAGTACAGACAATTCTTGGAATCTTGCGACTTCATCAAGGCAAGCAGCCAGTGGCGAAAAGTTCAAGATCGCTTAGAGGCTGACGAACGGTGCTCACGTCTTGAAAAAATTGATCGTTTGGAGATTTTCCAGGAATATCTACGCGATCTAGAGAAGGAAGAGGAAGAGCAGAGGAGGATACAGAAGGAAGAATTGAGGAAGGCAGAACGTAAAAACCGTGATGAGTTCCGCAAATTGATGGATGATCATATTGCTGCAGGCACTCTTTCAGCTACAACTCACTGGCGAGATTACATCCTGAAGGTTAAAGATCTACCTGCATATGTAGCAGTGGTATCAAACACCTCTGGTTCAACTCCAAAAGATTTATTTGAAGATGTTATTGAAGAGCTAGAGAAGCAATATCAAAGTGACAAAATTCGAATAAAAGACGTGGTGAAGTCGGGAAAGATTGTGCTTTCATCAACCTGGGACCTTGAAGCCTTCAAAACTGCTATATCGAATGATATTGGCTCTCCACCCATATCAGATGCAAACTTAATGCTAGTATTTGAGGAGCTAATGGAACGCGTGAGGGAAAAGGAGGAGAAAGAAGCTAAAAGGCATAAACGTCTTGCAGATGATTTCTTTCATCTCTTATCTTCTATGAAGGAGATAACTCCATCTTCTAAATGGGAGGACTGTAAATCACTTCTTGAAGTTAGTTCAGAGTACAGTGCTATTGGTGAAGAGAGCTCATGCAAGGAGATATTCGATAAACACATAATGCAATTGAAAGAACAAGCAAAAGAGAAAGAGAGAAAACGAAAGGAAGAGAAGGAGAGGAAGGAAAAGGACAGGGAAGAAAGAGAGAGGAGAAGGTCGAAGCAGAGAAGGGAAAGAGGGGTAGGATATGAAAGAGATAAAGAGGATCACCTTATGAAAGACGGAGAAGACACTGAAATTATTGACATAATGGAGGTTAATGATTCCAAAGAGAAAAGGAGATCAGGAAAAGACGATGATAAGAAACATAGGAAGAGGCATCAAAGTGATGAAGACCATCAAACAAATGGAAGTGAGAATGATCGGTCTAAAAGGTCTCATGGATCTAGCAGCGACCACAAGAGATCAAGACGGGCATCTGGTCCTGAATCAGATACTGAAAGCAGGCACAGAAGACATAAGAGAGATCACCGCAATGGTTCTCGGAGAAGTGGTGATCATGAGGAGCTAGAAGATGGGGAATTTGGTGATGTTGGGGAAAGTCGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005685 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006928 GO:0006996 GO:0007010 GO:0008150 GO:0008152 GO:0008360 GO:0008380 GO:0009987 GO:0010467 GO:0010564 GO:0016043 GO:0016070 GO:0016071 GO:0016363 GO:0016477 GO:0016604 GO:0016607 GO:0022603 GO:0022604 GO:0030532 GO:0031974 GO:0031981 GO:0032465 GO:0032991 GO:0034399 GO:0034641 GO:0040011 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051302 GO:0051674 GO:0051726 GO:0065007 GO:0065008 GO:0070013 GO:0070064 GO:0071004 GO:0071010 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097525 GO:0120114 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

FF WW

Protein Analysis

1022

Amino Acids

116.74

Weight (kDa)

6.27

Isoelectric Point (pI)

63.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	223 - 251	2.5e-08	WW domain
WW	PF00397	266 - 292	5.7e-08	WW domain
FF	PF01846	467 - 516	1.8e-16	FF domain
FF	PF01846	534 - 584	5.8e-14	FF domain
FF	PF01846	676 - 731	9.7e-08	FF domain
FF_PRPF40A	PF25432	739 - 798	7.4e-20	PRPF40A-like, FF domain
FF	PF01846	812 - 859	1.5e-08	FF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003687)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g03330 FvH4_2g03330 FvH4_2g03330 FvH4_2g03330 FvH4_2g03330 FvH4_2g03330 FvH4_2g03330 FvH4_2g03361 FvH4_2g03361
malus_domestica	MD05G1105600.v1.1
prunus_persica	Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1 Prupe.8G150300_v2.0.a1
pyrus_communis	pycom05g10260
rosa_chinensis	RchiOBHm_Chr6g0248471
rosa_laevigata	RLG00000015152
rosa_multiflora	Rmu_sc0002553.1_g000012
rosa_roxburghii	Rroxscaffold_7G00213270
rosa_rugosa	Rorug05G0531500
rosa_samantha	Rh6AG047400 Rh6BG043700 Rh6CG040400 Rh6DG036900
rosa_wichuraiana	Rw6G004110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1159, 2140
AccB1I	GGYRCC	1 cut(s)	1042
AccB7I	CCANNNNNTGG	4 cut(s)	263, 278, 1662, 1813
AccBSI	CCGCTC	1 cut(s)	1525
AccI	GTMKAC	1 cut(s)	1433
AccII	CGCG	2 cut(s)	1959, 2385
AciI	CCGC	7 cut(s)	83, 233, 1525, 1585, 1729, 2044, 2992
AclI	AACGTT	1 cut(s)	1699
AclWI	GGATC	7 cut(s)	1229, 1361, 1453, 1470, 1595, 2733, 2911
AcoI	YGGCCR	3 cut(s)	3, 104, 407
AcsI	RAATTY	7 cut(s)	157, 456, 806, 1243, 1259, 2232, 3041
AcuI	CTGAAG	5 cut(s)	603, 905, 1209, 1748, 2135
AcvI	CACGTG	1 cut(s)	1607
AfaI	GTAC	5 cut(s)	351, 579, 1290, 1820, 2528
AfiI	CCNNNNNNNGG	8 cut(s)	53, 89, 263, 278, 844, 1036, 1662, 1813
AflII	CTTAAG	1 cut(s)	394
AflIII	ACRYGT	1 cut(s)	2383
AhdI	GACNNNNNGTC	1 cut(s)	608
AhlI	ACTAGT	1 cut(s)	1058
AjiI	CACGTC	2 cut(s)	1913, 2248
AjnI	CCWGG	3 cut(s)	271, 1944, 2282
AjuI	GAANNNNNNNTTGG	2 cut(s)	213, 245
AloI	GAACNNNNNNTCC	4 cut(s)	165, 197, 1788, 1820
Alw21I	GWGCWC	2 cut(s)	1910, 2552
Alw26I	GTCTC	3 cut(s)	1102, 1719, 2901
AlwI	GGATC	7 cut(s)	1229, 1361, 1453, 1470, 1595, 2733, 2911
AlwNI	CAGNNNCTG	1 cut(s)	2957
AoxI	GGCC	8 cut(s)	3, 41, 104, 274, 407, 891, 1467, 1551
ApeKI	GCWGC	9 cut(s)	31, 244, 286, 586, 1193, 1217, 1860, 2069, 2910
ApoI	RAATTY	7 cut(s)	157, 456, 806, 1243, 1259, 2232, 3041
AseI	ATTAAT	2 cut(s)	1335, 1485
AspS9I	GGNCC	7 cut(s)	56, 266, 892, 1467, 1552, 2287, 2942
AsuC2I	CCSGG	1 cut(s)	895
AsuII	TTCGAA	1 cut(s)	2236
AvaII	GGWCC	4 cut(s)	56, 266, 2287, 2942
AxyI	CCTNAGG	2 cut(s)	876, 933
BaeGI	GKGCMC	1 cut(s)	403
BaeI	ACNNNNGTAYC	2 cut(s)	948, 981
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	1042
BanII	GRGCYC	1 cut(s)	2552
BarI	GAAGNNNNNNTAC	2 cut(s)	1272, 1304
BbrPI	CACGTG	1 cut(s)	1607
BbsI	GAAGAC	3 cut(s)	2754, 2859, 2980
Bbv12I	GWGCWC	2 cut(s)	1910, 2552
BbvCI	CCTCAGC	1 cut(s)	354
BbvI	GCAGC	9 cut(s)	43, 256, 273, 598, 1180, 1204, 1872, 2056, 2922
BceAI	ACGGC	1 cut(s)	422
BcgI	CGANNNNNNTGC	4 cut(s)	20, 54, 108, 142
BciT130I	CCWGG	3 cut(s)	273, 1946, 2284
BciVI	GTATCC	1 cut(s)	1986
BclI	TGATCA	2 cut(s)	2059, 3016
BcnI	CCSGG	1 cut(s)	895
BcoDI	GTCTC	3 cut(s)	1102, 1719, 2901
BcuI	ACTAGT	1 cut(s)	1058
BfaI	CTAG	6 cut(s)	1059, 1964, 2207, 2360, 2907, 3029
BfmI	CTRYAG	3 cut(s)	960, 1218, 2070
BfrI	CTTAAG	1 cut(s)	394
BfuAI	ACCTGC	2 cut(s)	1159, 2140
BfuI	GTATCC	1 cut(s)	1986
BglI	GCCNNNNNGGC	1 cut(s)	40
BglII	AGATCT	2 cut(s)	260, 2125
Bme1390I	CCNGG	4 cut(s)	273, 895, 1946, 2284
Bme18I	GGWCC	4 cut(s)	56, 266, 2287, 2942
BmeRI	GACNNNNNGTC	1 cut(s)	608
BmgBI	CACGTC	2 cut(s)	1913, 2248
BmgT120I	GGNCC	7 cut(s)	56, 266, 892, 1467, 1552, 2287, 2942
BmiI	GGNNCC	3 cut(s)	1044, 1305, 2288
BmrFI	CCNGG	4 cut(s)	273, 895, 1946, 2284
BpiI	GAAGAC	3 cut(s)	2754, 2859, 2980
BpmI	CTGGAG	2 cut(s)	612, 794
Bpu10I	CCTNAGC	1 cut(s)	354
Bpu14I	TTCGAA	1 cut(s)	2236
BpuEI	CTTGAG	1 cut(s)	999
BpuMI	CCSGG	1 cut(s)	895
BsaAI	YACGTR	1 cut(s)	1607
BsaI	GGTCTC	2 cut(s)	1719, 2901
BsaJI	CCNNGG	6 cut(s)	87, 277, 404, 528, 1275, 2283
BsaXI	ACNNNNNCTCC	4 cut(s)	1788, 1818, 2998, 3028
Bsc4I	CCNNNNNNNGG	8 cut(s)	53, 89, 263, 278, 844, 1036, 1662, 1813
Bse21I	CCTNAGG	2 cut(s)	876, 933
Bse3DI	GCAATG	2 cut(s)	1341, 3001
BseBI	CCWGG	3 cut(s)	273, 1946, 2284
BseDI	CCNNGG	6 cut(s)	87, 277, 404, 528, 1275, 2283
BseGI	GGATG	6 cut(s)	70, 524, 639, 1652, 2062, 2109
BseLI	CCNNNNNNNGG	8 cut(s)	53, 89, 263, 278, 844, 1036, 1662, 1813
BseMI	GCAATG	2 cut(s)	1341, 3001
BseMII	CTCAG	6 cut(s)	239, 368, 867, 947, 1268, 1653
BseRI	GAGGAG	7 cut(s)	60, 1592, 2002, 2384, 2414, 2683, 3038
BseSI	GKGCMC	1 cut(s)	403
BseXI	GCAGC	9 cut(s)	43, 256, 273, 598, 1180, 1204, 1872, 2056, 2922
BsgI	GTGCAG	1 cut(s)	263
Bsh1236I	CGCG	2 cut(s)	1959, 2385
BshFI	GGCC	8 cut(s)	5, 43, 106, 276, 409, 893, 1469, 1553
BshNI	GGYRCC	1 cut(s)	1042
BsiHKAI	GWGCWC	2 cut(s)	1910, 2552
BsiSI	CCGG	2 cut(s)	103, 895
BslFI	GGGAC	2 cut(s)	546, 2300
BslI	CCNNNNNNNGG	8 cut(s)	53, 89, 263, 278, 844, 1036, 1662, 1813
BsmAI	GTCTC	3 cut(s)	1102, 1719, 2901
BsmFI	GGGAC	2 cut(s)	546, 2300
BsmI	GAATGC	1 cut(s)	1640
BsnI	GGCC	8 cut(s)	5, 43, 106, 276, 409, 893, 1469, 1553
Bso31I	GGTCTC	2 cut(s)	1719, 2901
Bsp119I	TTCGAA	1 cut(s)	2236
Bsp1286I	GDGCHC	3 cut(s)	403, 1910, 2552
Bsp1407I	TGTACA	1 cut(s)	1288
Bsp19I	CCATGG	2 cut(s)	87, 277
BspACI	CCGC	7 cut(s)	83, 233, 1525, 1585, 1729, 2044, 2992
BspANI	GGCC	8 cut(s)	5, 43, 106, 276, 409, 893, 1469, 1553
BspCNI	CTCAG	6 cut(s)	238, 367, 868, 946, 1267, 1652
BspFNI	CGCG	2 cut(s)	1959, 2385
BspHI	TCATGA	1 cut(s)	3019
BspLI	GGNNCC	3 cut(s)	1044, 1305, 2288
BspMAI	CTGCAG	2 cut(s)	1222, 2074
BspMI	ACCTGC	2 cut(s)	1159, 2140
BspPI	GGATC	7 cut(s)	1229, 1361, 1453, 1470, 1595, 2733, 2911
BspQI	GCTCTTC	2 cut(s)	1974, 2196
BspT104I	TTCGAA	1 cut(s)	2236
BspT107I	GGYRCC	1 cut(s)	1042
BspTI	CTTAAG	1 cut(s)	394
BspTNI	GGTCTC	2 cut(s)	1719, 2901
BsrBI	CCGCTC	1 cut(s)	1525
BsrDI	GCAATG	2 cut(s)	1341, 3001
BsrGI	TGTACA	1 cut(s)	1288
BssECI	CCNNGG	6 cut(s)	87, 277, 404, 528, 1275, 2283
BssT1I	CCWWGG	2 cut(s)	87, 277
Bst2UI	CCWGG	3 cut(s)	273, 1946, 2284
Bst4CI	ACNGT	8 cut(s)	675, 964, 1081, 1204, 1905, 2033, 2498, 2531
Bst6I	CTCTTC	8 cut(s)	42, 169, 1968, 1974, 2196, 2538, 2622, 2828
BstAFI	CTTAAG	1 cut(s)	394
BstAPI	GCANNNNNTGC	1 cut(s)	241
BstAUI	TGTACA	1 cut(s)	1288
BstBAI	YACGTR	1 cut(s)	1607
BstBI	TTCGAA	1 cut(s)	2236
BstC8I	GCNNGC	6 cut(s)	510, 637, 1533, 1858, 2074, 2966
BstDEI	CTNAG	7 cut(s)	225, 354, 876, 933, 1254, 1639, 1888
BstDSI	CCRYGG	3 cut(s)	87, 277, 404
BstF5I	GGATG	6 cut(s)	70, 524, 639, 1652, 2062, 2109
BstFNI	CGCG	2 cut(s)	1959, 2385
BstMAI	GTCTC	3 cut(s)	1102, 1719, 2901
BstNI	CCWGG	3 cut(s)	273, 1946, 2284
BstNSI	RCATGY	2 cut(s)	170, 512
BstSCI	CCNGG	4 cut(s)	271, 893, 1944, 2282
BstSFI	CTRYAG	3 cut(s)	960, 1218, 2070
BstSLI	GKGCMC	1 cut(s)	403
BstUI	CGCG	2 cut(s)	1959, 2385
BstV1I	GCAGC	9 cut(s)	43, 256, 273, 598, 1180, 1204, 1872, 2056, 2922
BstV2I	GAAGAC	3 cut(s)	2754, 2859, 2980
BstX2I	RGATCY	5 cut(s)	260, 1234, 1445, 2125, 2903
BstXI	CCANNNNNNTGG	1 cut(s)	675
BstYI	RGATCY	5 cut(s)	260, 1234, 1445, 2125, 2903
Bsu36I	CCTNAGG	2 cut(s)	876, 933
BsuI	GTATCC	1 cut(s)	1986
BsuRI	GGCC	8 cut(s)	5, 43, 106, 276, 409, 893, 1469, 1553
BtgI	CCRYGG	3 cut(s)	87, 277, 404
BtrI	CACGTC	2 cut(s)	1913, 2248
BtsCI	GGATG	6 cut(s)	70, 524, 639, 1652, 2062, 2109
BtsI	GCAGTG	1 cut(s)	2151
BtsIMutI	CAGTG	9 cut(s)	246, 591, 680, 770, 1871, 2095, 2151, 2536, 2751
BveI	ACCTGC	2 cut(s)	1159, 2140
Cac8I	GCNNGC	6 cut(s)	510, 637, 1533, 1858, 2074, 2966
CaiI	CAGNNNCTG	1 cut(s)	2957
CciI	TCATGA	1 cut(s)	3019
Cfr13I	GGNCC	7 cut(s)	56, 266, 892, 1467, 1552, 2287, 2942
Csp6I	GTAC	5 cut(s)	350, 578, 1289, 1819, 2527
CspCI	CAANNNNNGTGG	4 cut(s)	604, 639, 1045, 1080
CviQI	GTAC	5 cut(s)	350, 578, 1289, 1819, 2527
DdeI	CTNAG	7 cut(s)	225, 354, 876, 933, 1254, 1639, 1888
DraI	TTTAAA	1 cut(s)	1417
DriI	GACNNNNNGTC	1 cut(s)	608
EaeI	YGGCCR	3 cut(s)	3, 104, 407
Eam1104I	CTCTTC	8 cut(s)	42, 169, 1968, 1974, 2196, 2538, 2622, 2828
Eam1105I	GACNNNNNGTC	1 cut(s)	608
EarI	CTCTTC	8 cut(s)	42, 169, 1968, 1974, 2196, 2538, 2622, 2828
EciI	GGCGGA	1 cut(s)	1600
Ecl136II	GAGCTC	1 cut(s)	2550
Eco130I	CCWWGG	2 cut(s)	87, 277
Eco24I	GRGCYC	1 cut(s)	2552
Eco31I	GGTCTC	2 cut(s)	1719, 2901
Eco47I	GGWCC	4 cut(s)	56, 266, 2287, 2942
Eco53kI	GAGCTC	1 cut(s)	2550
Eco57I	CTGAAG	5 cut(s)	603, 905, 1209, 1748, 2135
Eco72I	CACGTG	1 cut(s)	1607
Eco81I	CCTNAGG	2 cut(s)	876, 933
EcoICRI	GAGCTC	1 cut(s)	2550
EcoO109I	RGGNCCY	1 cut(s)	2287
EcoRII	CCWGG	3 cut(s)	271, 1944, 2282
EcoT14I	CCWWGG	2 cut(s)	87, 277
EcoT22I	ATGCAT	2 cut(s)	790, 1414
EcoT38I	GRGCYC	1 cut(s)	2552
ErhI	CCWWGG	2 cut(s)	87, 277
FaqI	GGGAC	2 cut(s)	546, 2300
FauNDI	CATATG	1 cut(s)	2137
FbaI	TGATCA	2 cut(s)	2059, 3016
FblI	GTMKAC	1 cut(s)	1433
FokI	GGATG	6 cut(s)	77, 511, 626, 1639, 2069, 2096
FriOI	GRGCYC	1 cut(s)	2552
FspBI	CTAG	6 cut(s)	1059, 1964, 2207, 2360, 2907, 3029
GsuI	CTGGAG	2 cut(s)	612, 794
HaeIII	GGCC	8 cut(s)	5, 43, 106, 276, 409, 893, 1469, 1553
HapII	CCGG	2 cut(s)	103, 895
HincII	GTYRAC	2 cut(s)	1207, 1647
HindII	GTYRAC	2 cut(s)	1207, 1647
HindIII	AAGCTT	1 cut(s)	1533
HinfI	GANTC	7 cut(s)	476, 664, 857, 1430, 1835, 2783, 2948
HpaI	GTTAAC	1 cut(s)	1647
HpaII	CCGG	2 cut(s)	103, 895
Hpy166II	GTNNAC	5 cut(s)	269, 599, 1207, 1434, 1647
Hpy8I	GTNNAC	5 cut(s)	269, 599, 1207, 1434, 1647
HpyCH4III	ACNGT	8 cut(s)	675, 964, 1081, 1204, 1905, 2033, 2498, 2531
HpyCH4IV	ACGT	7 cut(s)	1606, 1699, 1798, 1912, 2023, 2247, 2425
HpyF3I	CTNAG	7 cut(s)	225, 354, 876, 933, 1254, 1639, 1888
HpySE526I	ACGT	7 cut(s)	1606, 1699, 1798, 1912, 2023, 2247, 2425
Ksp22I	TGATCA	2 cut(s)	2059, 3016
KspAI	GTTAAC	1 cut(s)	1647
LguI	GCTCTTC	2 cut(s)	1974, 2196
LmnI	GCTCC	5 cut(s)	73, 631, 1809, 2371, 3025
Lsp1109I	GCAGC	9 cut(s)	43, 256, 273, 598, 1180, 1204, 1872, 2056, 2922
MaeI	CTAG	6 cut(s)	1059, 1964, 2207, 2360, 2907, 3029
MaeII	ACGT	7 cut(s)	1606, 1699, 1798, 1912, 2023, 2247, 2425
MaeIII	GTNAC	7 cut(s)	249, 610, 847, 1156, 1198, 1318, 2225
MbiI	CCGCTC	1 cut(s)	1525
MfeI	CAATTG	3 cut(s)	503, 1362, 2585
MflI	RGATCY	5 cut(s)	260, 1234, 1445, 2125, 2903
MhlI	GDGCHC	3 cut(s)	403, 1910, 2552
MlsI	TGGCCA	1 cut(s)	5
MluI	ACGCGT	1 cut(s)	2383
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	470, 1439
MmeI	TCCRAC	3 cut(s)	151, 667, 1608
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	790, 1414
MscI	TGGCCA	1 cut(s)	5
MslI	CAYNNNNRTG	4 cut(s)	143, 1475, 2844, 2994
Msp20I	TGGCCA	1 cut(s)	5
MspCI	CTTAAG	1 cut(s)	394
MspI	CCGG	2 cut(s)	103, 895
MspR9I	CCNGG	4 cut(s)	273, 895, 1946, 2284
MunI	CAATTG	3 cut(s)	503, 1362, 2585
Mva1269I	GAATGC	1 cut(s)	1640
MvaI	CCWGG	3 cut(s)	273, 1946, 2284
MvnI	CGCG	2 cut(s)	1959, 2385
NciI	CCSGG	1 cut(s)	895
NcoI	CCATGG	2 cut(s)	87, 277
NdeI	CATATG	1 cut(s)	2137
NlaIV	GGNNCC	3 cut(s)	1044, 1305, 2288
NmuCI	GTSAC	5 cut(s)	610, 847, 1198, 1318, 2225
NsiI	ATGCAT	2 cut(s)	790, 1414
NspI	RCATGY	2 cut(s)	170, 512
NspV	TTCGAA	1 cut(s)	2236
PaeI	GCATGC	1 cut(s)	512
PagI	TCATGA	1 cut(s)	3019
PciSI	GCTCTTC	2 cut(s)	1974, 2196
PcsI	WCGNNNNNNNCGW	2 cut(s)	1909, 2029
PctI	GAATGC	1 cut(s)	1640
PfeI	GAWTC	5 cut(s)	664, 857, 1835, 2783, 2948
PflMI	CCANNNNNTGG	4 cut(s)	263, 278, 1662, 1813
PfoI	TCCNGGA	1 cut(s)	1944
PleI	GAGTC	2 cut(s)	470, 1438
PmaCI	CACGTG	1 cut(s)	1607
PmlI	CACGTG	1 cut(s)	1607
PpsI	GAGTC	2 cut(s)	470, 1438
Ppu21I	YACGTR	1 cut(s)	1607
PpuMI	RGGWCCY	1 cut(s)	2287
PshBI	ATTAAT	2 cut(s)	1335, 1485
Psp124BI	GAGCTC	1 cut(s)	2552
Psp1406I	AACGTT	1 cut(s)	1699
Psp5II	RGGWCCY	1 cut(s)	2287
Psp6I	CCWGG	3 cut(s)	271, 1944, 2282
PspCI	CACGTG	1 cut(s)	1607
PspGI	CCWGG	3 cut(s)	271, 1944, 2282
PspN4I	GGNNCC	3 cut(s)	1044, 1305, 2288
PspPI	GGNCC	7 cut(s)	56, 266, 892, 1467, 1552, 2287, 2942
PspPPI	RGGWCCY	1 cut(s)	2287
PstI	CTGCAG	2 cut(s)	1222, 2074
PstNI	CAGNNNCTG	1 cut(s)	2957
PsuI	RGATCY	5 cut(s)	260, 1234, 1445, 2125, 2903
RsaI	GTAC	5 cut(s)	351, 579, 1290, 1820, 2528
RsaNI	GTAC	5 cut(s)	350, 578, 1289, 1819, 2527
RseI	CAYNNNNRTG	4 cut(s)	143, 1475, 2844, 2994
SacI	GAGCTC	1 cut(s)	2552
SapI	GCTCTTC	2 cut(s)	1974, 2196
Sau96I	GGNCC	7 cut(s)	56, 266, 892, 1467, 1552, 2287, 2942
SchI	GAGTC	2 cut(s)	470, 1439
ScrFI	CCNGG	4 cut(s)	273, 895, 1946, 2284
SduI	GDGCHC	3 cut(s)	403, 1910, 2552
SfcI	CTRYAG	3 cut(s)	960, 1218, 2070
SfuI	TTCGAA	1 cut(s)	2236
SinI	GGWCC	4 cut(s)	56, 266, 2287, 2942
SmiMI	CAYNNNNRTG	4 cut(s)	143, 1475, 2844, 2994
SmlI	CTYRAG	2 cut(s)	394, 1014
SmoI	CTYRAG	2 cut(s)	394, 1014
SpeI	ACTAGT	1 cut(s)	1058
SphI	GCATGC	1 cut(s)	512
SsiI	CCGC	7 cut(s)	83, 233, 1525, 1585, 1729, 2044, 2992
SspI	AATATT	1 cut(s)	20
SspMI	CTAG	6 cut(s)	1059, 1964, 2207, 2360, 2907, 3029
SstI	GAGCTC	1 cut(s)	2552
StyD4I	CCNGG	4 cut(s)	271, 893, 1944, 2282
StyI	CCWWGG	2 cut(s)	87, 277
TaaI	ACNGT	8 cut(s)	675, 964, 1081, 1204, 1905, 2033, 2498, 2531
TaiI	ACGT	7 cut(s)	1609, 1702, 1801, 1915, 2026, 2250, 2428
TaqI	TCGA	6 cut(s)	73, 479, 2236, 2315, 2568, 2678
TaqII	GACCGA	2 cut(s)	907, 2874
TatI	WGTACW	3 cut(s)	1288, 1818, 2526
TauI	GCSGC	1 cut(s)	85
TfiI	GAWTC	5 cut(s)	664, 857, 1835, 2783, 2948
TscAI	CASTG	9 cut(s)	246, 598, 680, 777, 1871, 2102, 2151, 2536, 2758
TseFI	GTSAC	5 cut(s)	610, 847, 1198, 1318, 2225
TseI	GCWGC	9 cut(s)	31, 244, 286, 586, 1193, 1217, 1860, 2069, 2910
Tsp45I	GTSAC	5 cut(s)	610, 847, 1198, 1318, 2225
TspGWI	ACGGA	1 cut(s)	2757
TspRI	CASTG	9 cut(s)	246, 598, 680, 777, 1871, 2102, 2151, 2536, 2758
Van91I	CCANNNNNTGG	4 cut(s)	263, 278, 1662, 1813
Vha464I	CTTAAG	1 cut(s)	394
VpaK11BI	GGWCC	4 cut(s)	56, 266, 2287, 2942
VspI	ATTAAT	2 cut(s)	1335, 1485
XapI	RAATTY	7 cut(s)	157, 456, 806, 1243, 1259, 2232, 3041
XbaI	TCTAGA	1 cut(s)	1963
XceI	RCATGY	2 cut(s)	170, 512
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2484, 2864
XmiI	GTMKAC	1 cut(s)	1433
XspI	CTAG	6 cut(s)	1059, 1964, 2207, 2360, 2907, 3029
Zsp2I	ATGCAT	2 cut(s)	790, 1414

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.