Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007786

Physical Location & Seq

Genomic Coordinates Reverse (-)

23873427 .. 23877805

Gene Structure 4379 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1117800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3864 bp

ATGGAAAATGGTGATAAGGTTGCTGGTGGGTCTGAAGTGGGGGAGAACAAGAGTGTGGAAGTTGAGGTTGTTGAGGGGTCTAATGGACTCAAGGATGACGCCGAAGATGAGGTTTTCGAGGAGGCAATAGAGACACAGGAAAATTTGCAGGAGCAGGGGAGCAAAAATGATTTAGTGGATGCTGCTGCTGTTGGTGAAGAAAGAAAGGCTGAGATGGTTGGTGGTTTGGGGTTGGACTCTCTTATAGAGAGTCCTAATGTCGAAAACTTTGAAGAGGCAATTGAATTTCATGATGAGGTTGGGAAATCTGATGGTGATGATGGGGAGGAGGCAATCGTGGCTGGTGAAGTGAAGGTGGAAAACATGGTGGGTAAGAACAGCGCTGATGAGGGTGGAGGGCCAGGCGGAATTGATGATGGACAGACTATAAAAGAAGTAGTCAGTGAGGAAACAAATGGATTGACAGATGATGAGTTGGTGGGCAGCCAAGAAGATGGAGTAAAAGAGGTTACTCAGGTTGGGGCTGGTGGAGGAATAGCAGGATTGACAGGTGGGGATGAGGCTGATGTAAAGTCTGTAGTTCTTGAGAATGTGAAGTTTGAAAAAGTCAATTTTGAATCAAATGGACTTGCGGATGATGGATTGGTGGGTAGCCAAGACGTTGAGGTGAAAGAAGTTTCTGAGATTATTACTGGTGCGGAAGTAGCAGGTTTGACAGATGTGGATGAGGTTGATTCAAAGCCCAATGCAGTTCTTAAGAATGAGGAGCCTGAGAGGAATGATATTGAATGTTCAACTTCAGAGCCAGTGCCTGCAGATGAAAAATTAGATACTGAAGATCTTGATTCTCCTCAAGTAACTGAATTTACTAAGGAGATTTTGAAGGAAGCTGGTAATAGTCAGGAGTTAGAGGATAATTCCTTGAGCATTGAGAATCAAGATGAGAAGACTGTGGACCTAGCAAGTGCTTCAGATGGCGTTCCTTTGAAGCTTCAAGATGATAACGGTGTGGAACTGCATGATAGAAACATGGATACAGTGCATCAGGAAGGTCATAGTGCTGAATCAAATGATGCCACCCTTAGCATTGAAGAAAAGCAGGAGGAGGATAATAAGACTGAAAAACCGAAAGATACTTTGACTGTTACAGATGCAGAACATCAAGGTTTCAGTAATGGGGAAGTGAAGGATTCTTTCACTGTGCCAGGTTCAGAGCATCACGAGGAAAAATCTGAACCAAAAAGCGTTTCATCTGCTAAACAGCTGTCTGGGGAGGGTGGTGAGGAAAGGATTGTGACATCAGAAAGGGAGATCTCAGCTCTTTCTAAAACATCTGCAACTGAGAAAACAGAGAAGATTCAGGATGGGGCCACTAATTTGAGAACAGAAAGTAACAAAGTTGATCAACCTCAGCGAGCTGGTGAAATTGCATGTGAAGTTCGCGACAACATTGCTGTGCCTGAAGAGCCTGAAAAGAAAGAAAACATTCCGGGGGAAAAGGGAATCACTAAAGTGAACAAAGAACAAGAGATTCGGCCTGCACCAGCTCTTTCTTCATCTTTAAATTCTACACAACGAAGTCCCCCTCCTGCTCGTCCAGCTGGCCTTGGGCGTGCTGCCCCACTTTTGGAACCTTCTCCTAGGGTGGTACAACATCCTCGAGTAAATGGTACAATATCTCATGTGCAAAACCAGCAAATTGAGGACCCTGTTAATGGAGAGGCAGAGGAGTCTGATGAGACTCGTGAAAAGCTTCAGATGATAAGAGTGAAATTTTTACGTCTTGCACATAGACTTGGGCAGACTCCGCATAATGTTGTTGTGGCTCAGGTTTTGTACAGATTGGGATTAGCTGAGCAGCTGCGTGGTAGAAATGGGGGCCGTGTTGGTGCCTTTAGTTTTGACCGTGCAAGTGCCATGGCGGAACAGCTTGAGGCATCGGGGAATGAACCCCTTGATTTTGCTTGTACCATTATGGTTCTTGGAAAGTCAGGAGTTGGTAAAAGCGCAACTATCAATTCTATATTTGATGAAAAGAAGTTCACTACTGATGCTTTTCAGATGGGAACAAAGAAAGTTCAGGATGTTGTGGGTACTGTGCAAGGGATTAAGGTACGGGTCATTGACACACCTGGACTTTTACCTTCCTGGTCAGACCAGGGACAGAATGAGAAGACTCTCCTCAATGTTAAGCGCTTTATCAAGAAAACACCTCCAGATATTGTGTTGTATTTTGATAGGTTGGACATGCAGAGCAGAGATTTTAGTGATATGCCACTCTTGCGCACCATCACAGACATTTTCGGGGCCTCTATTTGGTTCAATGCAATTGTGGTTCTGACTCATGCTGGATCAGCTCCACCTGAGGGCCCAAATGGTGCCGCTTCTAGTTATGACATGTTTGTCACTTCACGATCTCATGTTGTTCAGCAAGCCATTCGTCAGGCAGCCGGGGATATGCGCCTCATGAATCCTGTTTCATTAGTAGAGAACCACTCCGCTTGCAGAACTAATAGGGCTGGGCAAAGAGTCTTGCCAAATGGTCAGGTTTGGAAGCCTCATTTGTTGTTGCTCTCCTTTGCATCCAAGATTCTGGCCGAAGCAAATGCATTATTGAAGTTGCAAGATAGTCCACCTGGAAAACCTTTTGCAACTCGAACTAGGGCACCTCCTTTACCTTTCTTGCTTTCATCACTCCTTCAGTCAAGACCACAGTTGAAGCTGCCTGAGGAGCAGTTTGGTGAGGATGACAGTCTAGATGATGATCTGGATGAATCTTCAGATTCTGATGATGAATCAGAATTTGATGAATTGCCCCCATTCAGACGTTTGACCAAGGCCCAGGTGGAAAAACTCTCCAAAGCACAGAAAAAGGCATATTTTGATGAGCTAGAGTACAGGGAAAAGCTCTTTATGAAGAAACAATTGAAGGAGGAAAAAAAGCGACGGAAGTTGATGAAAAAAATGGCAGCTGCAGCAAAGGAACTGCCAAGTGATTATGTCGAAAATGTTGAAGAAGAGAGTTCTGGTGCAGCATCTGTACCGATTCCCATGCCAGATTTGGCATTGCCTGCTTCCTTTGATTCTGATAATCCAACTCACCGTTACCGGTACCTAGATTCCTCCAACCAGTGGCTCGTAAGACCTGTTCTGGAACAGCATGGTTGGGATCATGATGTTGGTTATGAGGGTATAAATGCAGAAAGACTGTTTGTTGTTAAAGAAAAGATACCCTTATCTTTTTCTGGTCAGGTTACCAAGGATAAGAAGGACGCCAATGTCCAAATGGAAATTGCTACTTCAATAAAGCATGGAGAAGGGAAAGCGACTTCATTTGGGTTTGATATGCAGACTGTTGGAAAGGACTTGGCTTATACTCTACGAAGTGACACAAGGATCAGTAATTTTAGGAAGAATAAGGCTACTGCTGGTCTCTCAGTTACTCTCCTGGGTGATGCTTTATCGGCTGGTATGAAAGTTGAAGACAAATTTATTGCTAATAAACGGTTCCAATTGGTTATGACTGGTGGCGCAATGACTGCCCGTGGTGACGCTGCCTATGGTGGTAGTTTGGAGGCTCAGTTGAGAGACAAAGACCATCCTTTGGGTCGTTCCCTATCAACCCTTGGGCTCTCTGTCATGGATTGGCATGGAGATCTTGCCATAGGGTGCAATATACAGTCTCAGATTCCAGTTGGACGGCATACGAACCTGATTGCTCGTGCCAATTTGAACAATAGGGGGGCAGGACAACTCAGCTTGCGCTTAAATAGCTCTGAACAGCTTCAGTTAGCTTTGATTGGCCTTGTTCCTCTGCTCAGAAAGTTTTTCACCTTTCCTCAACAGTTGCAGTATGGACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006605 GO:0006810 GO:0006886 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0019867 GO:0019899 GO:0023052 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0033036 GO:0033365 GO:0034613 GO:0038023 GO:0042170 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051087 GO:0051117 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060089 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AIG1 TOC159_MAD

Protein Analysis

1288

Amino Acids

140.24

Weight (kDa)

4.75

Isoelectric Point (pI)

42.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AIG1	PF04548	656 - 803	7e-26	AIG1 family
MMR_HSR1	PF01926	658 - 729	3.1e-09	50S ribosome-binding GTPase
TOC159_MAD	PF11886	1014 - 1277	2.5e-125	Translocase of chloroplast 159/132, membrane anchor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007786)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16640 AT2G16640 AT2G16640 AT3G16620
fragaria_vesca	FvH4_2g01650 FvH4_2g01650
malus_domestica	MD05G1117800.v1.1 MD10G1120800.v1.1
prunus_persica	Prupe.8G162300_v2.0.a1 Prupe.8G162300_v2.0.a1
pyrus_communis	pycom05g11180 pycom10g10400
rosa_chinensis	RchiOBHm_Chr6g0244541
rosa_laevigata	RLG00000015350
rosa_multiflora	Rmu_co8516985.1_g000001 Rmu_sc0003679.1_g000009 Rmu_sc0017888.1_g000001
rosa_roxburghii	Rroxscaffold_7G00215770
rosa_rugosa	Rorug05G0509200
rosa_samantha	Rh6AG024900 Rh6BG019800 Rh6CG017300 Rh6DG018300
rosa_wichuraiana	Rw6G002030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	2402, 3278
Acc16I	TGCGCA	1 cut(s)	2285
Acc36I	ACCTGC	1 cut(s)	698
Acc65I	GGTACC	1 cut(s)	3111
AccB1I	GGYRCC	4 cut(s)	1889, 2378, 2665, 3111
AccB7I	CCANNNNNTGG	2 cut(s)	3132, 3604
AccII	CGCG	1 cut(s)	1443
AciI	CCGC	7 cut(s)	405, 632, 698, 1808, 1922, 2382, 2499
AclWI	GGATC	3 cut(s)	2359, 3177, 3404
AcoI	YGGCCR	1 cut(s)	2595
AcsI	RAATTY	7 cut(s)	142, 284, 863, 1564, 1772, 2801, 3488
AcuI	CTGAAG	9 cut(s)	54, 783, 855, 954, 1482, 1739, 2684, 2763, 3770
AcyI	GRCGYC	2 cut(s)	99, 3273
AfaI	GTAC	9 cut(s)	1650, 1672, 1838, 1969, 2095, 2115, 2897, 3042, 3113
AfeI	AGCGCT	2 cut(s)	382, 2195
AfiI	CCNNNNNNNGG	6 cut(s)	1627, 1715, 2316, 2366, 3132, 3604
AflII	CTTAAG	1 cut(s)	755
AflIII	ACRYGT	1 cut(s)	2397
AgeI	ACCGGT	1 cut(s)	3108
AjnI	CCWGG	8 cut(s)	400, 1204, 2131, 2147, 2157, 2635, 2841, 3447
AjuI	GAANNNNNNNTTGG	2 cut(s)	1231, 1263
AleI	CACNNNNGTG	1 cut(s)	1511
Alw26I	GTCTC	5 cut(s)	125, 1733, 3437, 3582, 3687
AlwI	GGATC	3 cut(s)	2359, 3177, 3404
AlwNI	CAGNNNCTG	3 cut(s)	812, 2786, 3038
Ama87I	CYCGRG	1 cut(s)	1659
Aor51HI	AGCGCT	2 cut(s)	382, 2195
ApaI	GGGCCC	1 cut(s)	2372
ApoI	RAATTY	7 cut(s)	142, 284, 863, 1564, 1772, 2801, 3488
AsiGI	ACCGGT	1 cut(s)	3108
Asp700I	GAANNNNTTC	3 cut(s)	1485, 1752, 3783
Asp718I	GGTACC	1 cut(s)	3111
AspA2I	CCTAGG	1 cut(s)	1640
AspLEI	GCGC	7 cut(s)	383, 2009, 2196, 2286, 2463, 3533, 3765
AspS9I	GGNCC	9 cut(s)	398, 955, 1368, 1705, 1879, 2307, 2368, 2369, 2839
AsuC2I	CCSGG	2 cut(s)	1491, 2452
AvaI	CYCGRG	1 cut(s)	1659
AvaII	GGWCC	2 cut(s)	955, 1705
AvrII	CCTAGG	1 cut(s)	1640
AxyI	CCTNAGG	2 cut(s)	2364, 2727
BaeGI	GKGCMC	2 cut(s)	2372, 2668
BanI	GGYRCC	4 cut(s)	1889, 2378, 2665, 3111
BanII	GRGCYC	2 cut(s)	2372, 3633
BauI	CACGAG	3 cut(s)	1220, 1743, 3720
BbsI	GAAGAC	3 cut(s)	953, 2180, 3489
BbvCI	CCTCAGC	1 cut(s)	1410
BceAI	ACGGC	2 cut(s)	1866, 3716
BcgI	CGANNNNNNTGC	8 cut(s)	1433, 1467, 2420, 2454, 2588, 2622, 3034, 3068
BciT130I	CCWGG	8 cut(s)	402, 1206, 2133, 2149, 2159, 2637, 2843, 3449
BciVI	GTATCC	1 cut(s)	1027
BclI	TGATCA	1 cut(s)	1402
BcnI	CCSGG	2 cut(s)	1491, 2452
BcoDI	GTCTC	5 cut(s)	125, 1733, 3437, 3582, 3687
BfaI	CTAG	7 cut(s)	959, 1641, 2388, 2661, 2756, 2891, 3116
BfmI	CTRYAG	3 cut(s)	576, 813, 2973
BfoI	RGCGCY	2 cut(s)	384, 2197
BfrI	CTTAAG	1 cut(s)	755
BfuAI	ACCTGC	1 cut(s)	698
BfuI	GTATCC	1 cut(s)	1027
BglII	AGATCT	3 cut(s)	838, 1311, 3655
BlnI	CCTAGG	1 cut(s)	1640
BlpI	GCTNAGC	1 cut(s)	1854
Bme18I	GGWCC	2 cut(s)	955, 1705
BmeT110I	CYCGRG	1 cut(s)	1659
BmgT120I	GGNCC	9 cut(s)	398, 955, 1368, 1705, 1879, 2307, 2368, 2369, 2839
BpiI	GAAGAC	3 cut(s)	953, 2180, 3489
BplI	GAGNNNNNCTC	2 cut(s)	2480, 2512
BpmI	CTGGAG	1 cut(s)	2199
Bpu10I	CCTNAGC	3 cut(s)	1082, 1410, 1827
Bpu1102I	GCTNAGC	1 cut(s)	1854
BpuEI	CTTGAG	5 cut(s)	74, 605, 837, 943, 1952
BpuMI	CCSGG	2 cut(s)	1491, 2452
BsaBI	GATNNNNATC	1 cut(s)	2763
BsaHI	GRCGYC	2 cut(s)	99, 3273
BsaI	GGTCTC	1 cut(s)	3437
BsaWI	WCCGGW	1 cut(s)	3108
BsaXI	ACNNNNNCTCC	2 cut(s)	3566, 3596
Bsc4I	CCNNNNNNNGG	6 cut(s)	1627, 1715, 2316, 2366, 3132, 3604
Bse118I	RCCGGY	1 cut(s)	3108
Bse1I	ACTGG	5 cut(s)	697, 806, 3130, 3529, 3692
Bse21I	CCTNAGG	2 cut(s)	2364, 2727
Bse3DI	GCAATG	3 cut(s)	1449, 3065, 3540
Bse8I	GATNNNNATC	1 cut(s)	2763
BseBI	CCWGG	8 cut(s)	402, 1206, 2133, 2149, 2159, 2637, 2843, 3449
BseJI	GATNNNNATC	1 cut(s)	2763
BseLI	CCNNNNNNNGG	6 cut(s)	1627, 1715, 2316, 2366, 3132, 3604
BseMI	GCAATG	3 cut(s)	1449, 3065, 3540
BseNI	ACTGG	5 cut(s)	697, 806, 3130, 3529, 3692
BseRI	GAGGAG	8 cut(s)	134, 341, 779, 840, 1118, 1742, 2171, 2744
BseSI	GKGCMC	2 cut(s)	2372, 2668
BseYI	CCCAGC	1 cut(s)	2519
BsgI	GTGCAG	2 cut(s)	1524, 3051
Bsh1236I	CGCG	1 cut(s)	1443
BshNI	GGYRCC	4 cut(s)	1889, 2378, 2665, 3111
BshTI	ACCGGT	1 cut(s)	3108
BsiHKCI	CYCGRG	1 cut(s)	1659
BsiSI	CCGG	3 cut(s)	1490, 2451, 3109
BslFI	GGGAC	2 cut(s)	1566, 2175
BslI	CCNNNNNNNGG	6 cut(s)	1627, 1715, 2316, 2366, 3132, 3604
BsmAI	GTCTC	5 cut(s)	125, 1733, 3437, 3582, 3687
BsmFI	GGGAC	2 cut(s)	1566, 2175
Bso31I	GGTCTC	1 cut(s)	3437
BsoBI	CYCGRG	1 cut(s)	1659
Bsp120I	GGGCCC	1 cut(s)	2368
Bsp1286I	GDGCHC	3 cut(s)	2372, 2668, 3633
Bsp1407I	TGTACA	1 cut(s)	1836
Bsp143I	GATC	9 cut(s)	838, 1311, 1402, 2351, 2414, 2764, 3169, 3396, 3655
Bsp1720I	GCTNAGC	1 cut(s)	1854
Bsp19I	CCATGG	1 cut(s)	1917
Bsp68I	TCGCGA	1 cut(s)	1443
BspACI	CCGC	7 cut(s)	405, 632, 698, 1808, 1922, 2382, 2499
BspFNI	CGCG	1 cut(s)	1443
BspHI	TCATGA	3 cut(s)	289, 2466, 3172
BspMAI	CTGCAG	2 cut(s)	817, 2977
BspMI	ACCTGC	1 cut(s)	698
BspPI	GGATC	3 cut(s)	2359, 3177, 3404
BspQI	GCTCTTC	1 cut(s)	1458
BspT107I	GGYRCC	4 cut(s)	1889, 2378, 2665, 3111
BspTI	CTTAAG	1 cut(s)	755
BspTNI	GGTCTC	1 cut(s)	3437
BsrDI	GCAATG	3 cut(s)	1449, 3065, 3540
BsrFI	RCCGGY	1 cut(s)	3108
BsrGI	TGTACA	1 cut(s)	1836
BsrI	ACTGG	5 cut(s)	697, 806, 3130, 3529, 3692
BssAI	RCCGGY	1 cut(s)	3108
BssMI	GATC	9 cut(s)	838, 1311, 1402, 2351, 2414, 2764, 3169, 3396, 3655
BssNI	GRCGYC	2 cut(s)	99, 3273
BssSI	CACGAG	3 cut(s)	1220, 1743, 3720
BssT1I	CCWWGG	6 cut(s)	1606, 1640, 1917, 2835, 3258, 3625
Bst2BI	CACGAG	3 cut(s)	1220, 1743, 3720
Bst2UI	CCWGG	8 cut(s)	402, 1206, 2133, 2149, 2159, 2637, 2843, 3449
Bst6I	CTCTTC	3 cut(s)	267, 1458, 3012
BstACI	GRCGYC	2 cut(s)	99, 3273
BstAFI	CTTAAG	1 cut(s)	755
BstAPI	GCANNNNNTGC	2 cut(s)	3071, 3539
BstAUI	TGTACA	1 cut(s)	1836
BstC8I	GCNNGC	9 cut(s)	813, 1416, 1539, 1603, 1614, 2433, 2503, 3072, 3761
BstDSI	CCRYGG	2 cut(s)	1917, 3544
BstEII	GGTNACC	1 cut(s)	3253
BstFNI	CGCG	1 cut(s)	1443
BstH2I	RGCGCY	2 cut(s)	384, 2197
BstHHI	GCGC	7 cut(s)	383, 2009, 2196, 2286, 2463, 3533, 3765
BstKTI	GATC	9 cut(s)	841, 1314, 1405, 2354, 2417, 2767, 3172, 3399, 3658
BstMAI	GTCTC	5 cut(s)	125, 1733, 3437, 3582, 3687
BstMBI	GATC	9 cut(s)	838, 1311, 1402, 2351, 2414, 2764, 3169, 3396, 3655
BstNI	CCWGG	8 cut(s)	402, 1206, 2133, 2149, 2159, 2637, 2843, 3449
BstNSI	RCATGY	3 cut(s)	1434, 2251, 2401
BstPI	GGTNACC	1 cut(s)	3253
BstSFI	CTRYAG	3 cut(s)	576, 813, 2973
BstSLI	GKGCMC	2 cut(s)	2372, 2668
BstUI	CGCG	1 cut(s)	1443
BstV2I	GAAGAC	3 cut(s)	953, 2180, 3489
BstX2I	RGATCY	3 cut(s)	838, 1311, 3655
BstXI	CCANNNNNNTGG	2 cut(s)	494, 2593
BstYI	RGATCY	3 cut(s)	838, 1311, 3655
Bsu36I	CCTNAGG	2 cut(s)	2364, 2727
BsuI	GTATCC	1 cut(s)	1027
BtgI	CCRYGG	2 cut(s)	1917, 3544
BtsIMutI	CAGTG	5 cut(s)	448, 813, 1044, 1197, 3137
BtuMI	TCGCGA	1 cut(s)	1443
BveI	ACCTGC	1 cut(s)	698
Cac8I	GCNNGC	9 cut(s)	813, 1416, 1539, 1603, 1614, 2433, 2503, 3072, 3761
CaiI	CAGNNNCTG	3 cut(s)	812, 2786, 3038
CciI	TCATGA	3 cut(s)	289, 2466, 3172
CfoI	GCGC	7 cut(s)	383, 2009, 2196, 2286, 2463, 3533, 3765
Cfr10I	RCCGGY	1 cut(s)	3108
Cfr13I	GGNCC	9 cut(s)	398, 955, 1368, 1705, 1879, 2307, 2368, 2369, 2839
CseI	GACGC	3 cut(s)	107, 3281, 3560
Csp6I	GTAC	9 cut(s)	1649, 1671, 1837, 1968, 2094, 2114, 2896, 3041, 3112
CspAI	ACCGGT	1 cut(s)	3108
CspCI	CAANNNNNGTGG	2 cut(s)	2483, 2518
CviQI	GTAC	9 cut(s)	1649, 1671, 1837, 1968, 2094, 2114, 2896, 3041, 3112
DpnI	GATC	9 cut(s)	840, 1313, 1404, 2353, 2416, 2766, 3171, 3398, 3657
DpnII	GATC	9 cut(s)	838, 1311, 1402, 2351, 2414, 2764, 3169, 3396, 3655
DraI	TTTAAA	1 cut(s)	1563
DrdI	GACNNNNNNGTC	2 cut(s)	2402, 3278
DseDI	GACNNNNNNGTC	2 cut(s)	2402, 3278
EaeI	YGGCCR	1 cut(s)	2595
Eam1104I	CTCTTC	3 cut(s)	267, 1458, 3012
EarI	CTCTTC	3 cut(s)	267, 1458, 3012
EciI	GGCGGA	2 cut(s)	420, 1937
Eco130I	CCWWGG	6 cut(s)	1606, 1640, 1917, 2835, 3258, 3625
Eco24I	GRGCYC	2 cut(s)	2372, 3633
Eco31I	GGTCTC	1 cut(s)	3437
Eco47I	GGWCC	2 cut(s)	955, 1705
Eco47III	AGCGCT	2 cut(s)	382, 2195
Eco57I	CTGAAG	9 cut(s)	54, 783, 855, 954, 1482, 1739, 2684, 2763, 3770
Eco81I	CCTNAGG	2 cut(s)	2364, 2727
Eco88I	CYCGRG	1 cut(s)	1659
Eco91I	GGTNACC	1 cut(s)	3253
EcoO109I	RGGNCCY	3 cut(s)	1705, 2307, 2368
EcoO65I	GGTNACC	1 cut(s)	3253
EcoRII	CCWGG	8 cut(s)	400, 1204, 2131, 2147, 2157, 2635, 2841, 3447
EcoT14I	CCWWGG	6 cut(s)	1606, 1640, 1917, 2835, 3258, 3625
EcoT22I	ATGCAT	1 cut(s)	2611
EcoT38I	GRGCYC	2 cut(s)	2372, 3633
ErhI	CCWWGG	6 cut(s)	1606, 1640, 1917, 2835, 3258, 3625
FalI	AAGNNNNNCTT	2 cut(s)	3218, 3250
FaqI	GGGAC	2 cut(s)	1566, 2175
FbaI	TGATCA	1 cut(s)	1402
FriOI	GRGCYC	2 cut(s)	2372, 3633
FspBI	CTAG	7 cut(s)	959, 1641, 2388, 2661, 2756, 2891, 3116
FspI	TGCGCA	1 cut(s)	2285
GlaI	GCGC	7 cut(s)	382, 2008, 2195, 2285, 2462, 3532, 3764
GsaI	CCCAGC	1 cut(s)	2523
GsuI	CTGGAG	1 cut(s)	2199
HaeII	RGCGCY	2 cut(s)	384, 2197
HapII	CCGG	3 cut(s)	1490, 2451, 3109
HgaI	GACGC	3 cut(s)	107, 3281, 3560
HhaI	GCGC	7 cut(s)	383, 2009, 2196, 2286, 2463, 3533, 3765
Hin1I	GRCGYC	2 cut(s)	99, 3273
Hin6I	GCGC	7 cut(s)	381, 2007, 2194, 2284, 2461, 3531, 3763
HinP1I	GCGC	7 cut(s)	381, 2007, 2194, 2284, 2461, 3531, 3763
HindIII	AAGCTT	2 cut(s)	989, 1751
HpaII	CCGG	3 cut(s)	1490, 2451, 3109
Hpy166II	GTNNAC	4 cut(s)	955, 1516, 2043, 2633
Hpy8I	GTNNAC	4 cut(s)	955, 1516, 2043, 2633
Hpy99I	CGWCG	1 cut(s)	2949
HpyCH4IV	ACGT	3 cut(s)	660, 1780, 2827
HpySE526I	ACGT	3 cut(s)	660, 1780, 2827
Hsp92I	GRCGYC	2 cut(s)	99, 3273
HspAI	GCGC	7 cut(s)	381, 2007, 2194, 2284, 2461, 3531, 3763
KpnI	GGTACC	1 cut(s)	3115
Ksp22I	TGATCA	1 cut(s)	1402
Kzo9I	GATC	9 cut(s)	838, 1311, 1402, 2351, 2414, 2764, 3169, 3396, 3655
LguI	GCTCTTC	1 cut(s)	1458
LmnI	GCTCC	5 cut(s)	151, 159, 766, 2362, 2731
MaeI	CTAG	7 cut(s)	959, 1641, 2388, 2661, 2756, 2891, 3116
MaeII	ACGT	3 cut(s)	660, 1780, 2827
MalI	GATC	9 cut(s)	840, 1313, 1404, 2353, 2416, 2766, 3171, 3398, 3657
MboI	GATC	9 cut(s)	838, 1311, 1402, 2351, 2414, 2764, 3169, 3396, 3655
MfeI	CAATTG	4 cut(s)	279, 2328, 2924, 3512
MflI	RGATCY	3 cut(s)	838, 1311, 3655
MhlI	GDGCHC	3 cut(s)	2372, 2668, 3633
MlyI	GAGTC	9 cut(s)	81, 230, 259, 1735, 1739, 1798, 2170, 2335, 2538
MmeI	TCCRAC	6 cut(s)	213, 2223, 3119, 3150, 3337, 3676
Mph1103I	ATGCAT	1 cut(s)	2611
MroXI	GAANNNNTTC	3 cut(s)	1485, 1752, 3783
MseI	TTAA	7 cut(s)	756, 1562, 1713, 2109, 2190, 3219, 3767
MslI	CAYNNNNRTG	2 cut(s)	1454, 1511
MspA1I	CMGCKG	4 cut(s)	1264, 1601, 1861, 2972
MspCI	CTTAAG	1 cut(s)	755
MspI	CCGG	3 cut(s)	1490, 2451, 3109
MunI	CAATTG	4 cut(s)	279, 2328, 2924, 3512
MvaI	CCWGG	8 cut(s)	402, 1206, 2133, 2149, 2159, 2637, 2843, 3449
MvnI	CGCG	1 cut(s)	1443
NciI	CCSGG	2 cut(s)	1491, 2452
NcoI	CCATGG	1 cut(s)	1917
NdeII	GATC	9 cut(s)	838, 1311, 1402, 2351, 2414, 2764, 3169, 3396, 3655
NmuCI	GTSAC	4 cut(s)	1294, 2404, 3386, 3548
NruI	TCGCGA	1 cut(s)	1443
NsbI	TGCGCA	1 cut(s)	2285
NsiI	ATGCAT	1 cut(s)	2611
NspI	RCATGY	3 cut(s)	1434, 2251, 2401
OliI	CACNNNNGTG	1 cut(s)	1511
PaeR7I	CTCGAG	1 cut(s)	1659
PagI	TCATGA	3 cut(s)	289, 2466, 3172
PciI	ACATGT	1 cut(s)	2397
PciSI	GCTCTTC	1 cut(s)	1458
PdmI	GAANNNNTTC	3 cut(s)	1485, 1752, 3783
PflMI	CCANNNNNTGG	2 cut(s)	3132, 3604
PinAI	ACCGGT	1 cut(s)	3108
PleI	GAGTC	9 cut(s)	81, 230, 258, 1735, 1738, 1798, 2170, 2335, 2537
PpsI	GAGTC	9 cut(s)	81, 230, 258, 1735, 1738, 1798, 2170, 2335, 2537
PpuMI	RGGWCCY	1 cut(s)	1705
PscI	ACATGT	1 cut(s)	2397
Psp5II	RGGWCCY	1 cut(s)	1705
Psp6I	CCWGG	8 cut(s)	400, 1204, 2131, 2147, 2157, 2635, 2841, 3447
PspEI	GGTNACC	1 cut(s)	3253
PspFI	CCCAGC	1 cut(s)	2519
PspGI	CCWGG	8 cut(s)	400, 1204, 2131, 2147, 2157, 2635, 2841, 3447
PspOMI	GGGCCC	1 cut(s)	2368
PspPI	GGNCC	9 cut(s)	398, 955, 1368, 1705, 1879, 2307, 2368, 2369, 2839
PspPPI	RGGWCCY	1 cut(s)	1705
PspXI	VCTCGAGB	1 cut(s)	1659
PsrI	GAACNNNNNNTAC	2 cut(s)	3132, 3164
PstI	CTGCAG	2 cut(s)	817, 2977
PstNI	CAGNNNCTG	3 cut(s)	812, 2786, 3038
PsuI	RGATCY	3 cut(s)	838, 1311, 3655
PvuII	CAGCTG	4 cut(s)	1264, 1601, 1861, 2972
RruI	TCGCGA	1 cut(s)	1443
RsaI	GTAC	9 cut(s)	1650, 1672, 1838, 1969, 2095, 2115, 2897, 3042, 3113
RsaNI	GTAC	9 cut(s)	1649, 1671, 1837, 1968, 2094, 2114, 2896, 3041, 3112
RseI	CAYNNNNRTG	2 cut(s)	1454, 1511
SapI	GCTCTTC	1 cut(s)	1458
SaqAI	TTAA	7 cut(s)	756, 1562, 1713, 2109, 2190, 3219, 3767
Sau3AI	GATC	9 cut(s)	838, 1311, 1402, 2351, 2414, 2764, 3169, 3396, 3655
Sau96I	GGNCC	9 cut(s)	398, 955, 1368, 1705, 1879, 2307, 2368, 2369, 2839
SchI	GAGTC	9 cut(s)	81, 230, 259, 1735, 1739, 1798, 2170, 2335, 2538
SduI	GDGCHC	3 cut(s)	2372, 2668, 3633
SfcI	CTRYAG	3 cut(s)	576, 813, 2973
Sfr274I	CTCGAG	1 cut(s)	1659
SinI	GGWCC	2 cut(s)	955, 1705
SlaI	CTCGAG	1 cut(s)	1659
SmiMI	CAYNNNNRTG	2 cut(s)	1454, 1511
SmlI	CTYRAG	7 cut(s)	89, 584, 755, 852, 922, 1659, 1931
SmoI	CTYRAG	7 cut(s)	89, 584, 755, 852, 922, 1659, 1931
SsiI	CCGC	7 cut(s)	405, 632, 698, 1808, 1922, 2382, 2499
SspMI	CTAG	7 cut(s)	959, 1641, 2388, 2661, 2756, 2891, 3116
StyI	CCWWGG	6 cut(s)	1606, 1640, 1917, 2835, 3258, 3625
TaiI	ACGT	3 cut(s)	663, 1783, 2830
TaqI	TCGA	5 cut(s)	117, 261, 1660, 2656, 3003
TatI	WGTACW	2 cut(s)	1836, 2895
TauI	GCSGC	1 cut(s)	2384
Tru1I	TTAA	7 cut(s)	756, 1562, 1713, 2109, 2190, 3219, 3767
Tru9I	TTAA	7 cut(s)	756, 1562, 1713, 2109, 2190, 3219, 3767
TscAI	CASTG	5 cut(s)	448, 813, 1044, 1204, 3137
TseFI	GTSAC	4 cut(s)	1294, 2404, 3386, 3548
Tsp45I	GTSAC	4 cut(s)	1294, 2404, 3386, 3548
TspGWI	ACGGA	1 cut(s)	2962
TspRI	CASTG	5 cut(s)	448, 813, 1044, 1204, 3137
Van91I	CCANNNNNTGG	2 cut(s)	3132, 3604
Vha464I	CTTAAG	1 cut(s)	755
VpaK11BI	GGWCC	2 cut(s)	955, 1705
XapI	RAATTY	7 cut(s)	142, 284, 863, 1564, 1772, 2801, 3488
XbaI	TCTAGA	1 cut(s)	2755
XceI	RCATGY	3 cut(s)	1434, 2251, 2401
XcmI	CCANNNNNNNNNTGG	3 cut(s)	2842, 3058, 3283
XhoI	CTCGAG	1 cut(s)	1659
XmaJI	CCTAGG	1 cut(s)	1640
XmnI	GAANNNNTTC	3 cut(s)	1485, 1752, 3783
XspI	CTAG	7 cut(s)	959, 1641, 2388, 2661, 2756, 2891, 3116
Zsp2I	ATGCAT	1 cut(s)	2611

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.