Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0007786

Physical Location & Seq

Genomic Coordinates Reverse (-)

14678565 .. 14681057

Gene Structure 2493 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g11180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2493 bp

ATGGAAAATGGTGATAAGAATGCTGGTGGGTCTGAAGTGGGGGAGAACAAGAGTGTGGAAGTTGAGGTTGTTGAGGAGAGGGTTGTAGAGGGGTCTAATGGACTGAAGGATGACGCTGAAGATGAGGTTTTTAAGGAGGCAATAAAGACACAGGAAAATTTGCAGCAGCAGGGGAGCAAAAAGGATTTAGTGGATGCTGCTGCTGTTGGTGAAGAAAGAAAGGCTGAGATGGTTGGTGGTTTGGGGTTGGACTCTCTTATAGAGAGTCCTAATGTCGAAAAGTTTGAAGAGGCAATTGAAGTTCCTGATGAGGTTGGGAAATCTGATGGTGATGATGGGGAGGAGGCAATCGTGGCTGGTGAAGTGAAGGTGGAAAACATGGTGGGTAAGAACAGCGATGATGAGGTTGGAGTGCCAGTTGGAATTGATGATGGACAGACTATAAAAGAAGTAGTCAGTGAGGAAACAAATGGATTGACAGATGATGGGTTGGTGGGCAGCCAAGAAGATGGAGTAAAAGAGGTTACTCAGGTTGGGGCTGGTGGAGGAATAGCAGGATTAACAGGTGGGGATGAGGCTGATGTAAAGTCTGTAGTTCTTGAGAATATGAAGTTTGAAAAAGTCAATTTTGAATCAAATGGACTTGCGGATGATGGATTGGTGGGTAGCCAAGACGTTGAGGTGAAAGAAGTTTCTGATATTATTACTGGTGCGGAAGTAGCAGGTTTTACAGATGTGGATGAGGTTGATTCAAAGCCCGATGCAGTTCTTAAGAATAAGGAGCCTGAGAGGAATGATATTGATAGTTCAACTTCAGAGCCAGTGCCTACAGATGAAAAATTAGATACTGAAGATCTTGATTCTCCTCAAGTAACTGAATTTACTAAGGAGATTTTGAAGGAAGCTGGTAATAGTCAGGAGTTAGAGGAGAATTCCTTGAGCATTGAGAATCAGGATGAGAAGACTGTGGAGCTAGCAAGTGCTTCAGATGGCGTTCCTTTGAAGCTTCAAGATGATAATGGTGTGGAACTGCATGATGGAAACATGGATACAGTGCATCAGGAAGGTCATAGTGCTGAATCAAATGATGCCACCCTTAGAATTGAAGAAAAGCAGGAGGAGGATAATAAGACTGAAGAACCGAAAGATACTTTGACTGTTACAGATGCTGAACATCAAGGTTTCAGTAATGGGGAAGTGAAGGACTCTTTCACTGTGCCGGGTTCAGAGCATCACGAGGAAAAATCTGAACCAAAAAGCGTTTCATCTGCTAAACAGCTGTCTGGGGAGGGTGGTGAGGAAAGGATTGTGACATCAGAAAGGGAGATCTCAGCTCTTTCTGAAACATCTGCAACTGAGAAAACAGAGAAGATTCAGGATGGAGCCACTAATTTGAGAACAAAGAGTAACAAAGTTGATCAACCTCAGCGAGTTGGTGAAATTGCATGTGAAGTTCACGACAACATTGCTGTGCCTGAAGAGCCTGAAAAGAAAGAAAACATTCAGGGAGAAAAGGGAATCACTAAAGTGAACAAAGAACAAGAGATTCAGCCTGCACCAGCTCTTTCTTCATCTTTAAATTCTACACAACCAAGTCCCCCTCCTGCTCGTCCAGCTGGCCTTGGTCGTGCTGCCCCACTTTTGGAACCTTCTCCTAGGGTGGTACAGCATCCTCGAGTAAATGGTACAATATCTCATGTGCAAAACCAGCAAATTGAGGACCCCGTTAATGGAGAGGCAGAGGAGTCTGATGAGACTCGTGAAAAGCTTCAGATGATAAGAGTGAAATTTTTACGTCTTGCACATAGACTTGGGCAGACTCCGCATAATGTTGTTGTGGCTCAGGTTTTGTACAGATTGGGATTAGCTGAGCAGCTGCGTGGTAGAAATGGGGGCCGTGTTGGTGCCTTTAGTTTTGACCGTGCAAGTGCAATGGCGGAACAGCTTGAGGCATCGGGGAATGAAGCCCTTGATTTTGCTTGTACCATTATGGTTCTTGGAAAGTCAGGAGTTGGTAAAAGCGCAACCATCAATTCTATATTTGATGAAAAGAAGTTCACTACTGATGCTTTTCAGATGGGGACAAAGAAAGTTCAGGATGTTGTGGGTACTGTGCAAGGGATTAAGGTACGGGTCATTGACACACCTGGACTTTTACCTTCCTGGTCAGACCAGGGACAGAATGAGAAGACTCTCCTCAATGTTAAGCGCTTTATCAAGAAAACACCTCCAGATATTGTGTTGTATTTTGATAGGTTGGACATGCAGAGCAGAGATTTTAGTGATATGCCACTCTTGCGCACCATCACAGACATTTTCGGGGCCTCTATTTGGTTCAATGCAATTGTGGTTCTGACTCATGCTGGATCGATCCTAAAGATCCACCTGAGGGCCCAAATGGTGCCGCTTCTAGTTATGACATGTTTGTCACTTCACGATCTCATGTTGTTCAGCAAGCCATTCGTCAGGCAGCCGGGGATATGCGCCTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006605 GO:0006810 GO:0006886 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0019867 GO:0019899 GO:0023052 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0033036 GO:0033365 GO:0034613 GO:0038023 GO:0042170 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051087 GO:0051117 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060089 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AIG1 TOC159_MAD

Protein Analysis

831

Amino Acids

89.72

Weight (kDa)

4.5

Isoelectric Point (pI)

39.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AIG1	PF04548	661 - 790	1.6e-25	AIG1 family
MMR_HSR1	PF01926	663 - 734	1.7e-09	50S ribosome-binding GTPase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007786)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16640 AT2G16640 AT2G16640 AT3G16620
fragaria_vesca	FvH4_2g01650 FvH4_2g01650
malus_domestica	MD05G1117800.v1.1 MD10G1120800.v1.1
prunus_persica	Prupe.8G162300_v2.0.a1 Prupe.8G162300_v2.0.a1
pyrus_communis	pycom05g11180 pycom10g10400
rosa_chinensis	RchiOBHm_Chr6g0244541
rosa_laevigata	RLG00000015350
rosa_multiflora	Rmu_co8516985.1_g000001 Rmu_sc0003679.1_g000009 Rmu_sc0017888.1_g000001
rosa_roxburghii	Rroxscaffold_7G00215770
rosa_rugosa	Rorug05G0509200
rosa_samantha	Rh6AG024900 Rh6BG019800 Rh6CG017300 Rh6DG018300
rosa_wichuraiana	Rw6G002030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2425
Acc16I	TGCGCA	1 cut(s)	2300
Acc36I	ACCTGC	1 cut(s)	713
AccB1I	GGYRCC	2 cut(s)	1904, 2401
AciI	CCGC	5 cut(s)	647, 713, 1823, 1937, 2405
AclWI	GGATC	3 cut(s)	2365, 2374, 2374
AcsI	RAATTY	5 cut(s)	157, 878, 931, 1579, 1787
AcuI	CTGAAG	9 cut(s)	54, 125, 138, 798, 870, 969, 1155, 1497, 1754
AfaI	GTAC	6 cut(s)	1665, 1687, 1853, 1984, 2110, 2130
AfeI	AGCGCT	1 cut(s)	2210
AfiI	CCNNNNNNNGG	4 cut(s)	1642, 1730, 2331, 2389
AflII	CTTAAG	1 cut(s)	770
AflIII	ACRYGT	1 cut(s)	2420
AjnI	CCWGG	3 cut(s)	2146, 2162, 2172
AjuI	GAANNNNNNNTTGG	2 cut(s)	1246, 1278
AleI	CACNNNNGTG	1 cut(s)	1526
Alw26I	GTCTC	1 cut(s)	1748
AlwI	GGATC	3 cut(s)	2365, 2374, 2374
AlwNI	CAGNNNCTG	1 cut(s)	1169
Ama87I	CYCGRG	1 cut(s)	1674
Aor51HI	AGCGCT	1 cut(s)	2210
AoxI	GGCC	4 cut(s)	1618, 1894, 2322, 2391
ApaI	GGGCCC	1 cut(s)	2395
ApeKI	GCWGC	9 cut(s)	163, 166, 197, 200, 498, 1631, 1873, 1876, 2470
ApoI	RAATTY	5 cut(s)	157, 878, 931, 1579, 1787
Asp700I	GAANNNNTTC	2 cut(s)	1500, 1767
AspA2I	CCTAGG	1 cut(s)	1655
AspLEI	GCGC	4 cut(s)	2024, 2211, 2301, 2486
AspS9I	GGNCC	5 cut(s)	1720, 1894, 2322, 2391, 2392
AsuC2I	CCSGG	2 cut(s)	1221, 2475
AsuHPI	GGTGA	7 cut(s)	23, 221, 341, 371, 694, 1307, 1448
AsuNHI	GCTAGC	1 cut(s)	973
AvaI	CYCGRG	1 cut(s)	1674
AvaII	GGWCC	1 cut(s)	1720
AvrII	CCTAGG	1 cut(s)	1655
AxyI	CCTNAGG	1 cut(s)	2387
BaeGI	GKGCMC	1 cut(s)	2395
BanI	GGYRCC	2 cut(s)	1904, 2401
BanII	GRGCYC	1 cut(s)	2395
BauI	CACGAG	2 cut(s)	1235, 1758
BbsI	GAAGAC	2 cut(s)	968, 2195
BbvCI	CCTCAGC	1 cut(s)	1425
BbvI	GCAGC	9 cut(s)	175, 178, 184, 187, 510, 1618, 1863, 1885, 2482
BceAI	ACGGC	1 cut(s)	1881
BcgI	CGANNNNNNTGC	4 cut(s)	1448, 1482, 2443, 2477
BciT130I	CCWGG	3 cut(s)	2148, 2164, 2174
BciVI	GTATCC	1 cut(s)	1042
BclI	TGATCA	1 cut(s)	1417
BcnI	CCSGG	2 cut(s)	1221, 2475
BcoDI	GTCTC	1 cut(s)	1748
BfaI	CTAG	3 cut(s)	974, 1656, 2411
BfmI	CTRYAG	2 cut(s)	591, 828
BfoI	RGCGCY	1 cut(s)	2212
BfrI	CTTAAG	1 cut(s)	770
BfuAI	ACCTGC	1 cut(s)	713
BfuI	GTATCC	1 cut(s)	1042
BglII	AGATCT	2 cut(s)	853, 1326
BlnI	CCTAGG	1 cut(s)	1655
BlpI	GCTNAGC	1 cut(s)	1869
Bme1390I	CCNGG	5 cut(s)	1221, 2148, 2164, 2174, 2475
Bme18I	GGWCC	1 cut(s)	1720
BmeT110I	CYCGRG	1 cut(s)	1674
BmgT120I	GGNCC	5 cut(s)	1720, 1894, 2322, 2391, 2392
BmiI	GGNNCC	9 cut(s)	783, 1384, 1647, 1722, 1895, 1906, 2323, 2393, 2403
BmrFI	CCNGG	5 cut(s)	1221, 2148, 2164, 2174, 2475
BmsI	GCATC	9 cut(s)	184, 751, 1066, 1078, 1156, 1240, 1678, 1961, 2056
BmtI	GCTAGC	1 cut(s)	977
BpiI	GAAGAC	2 cut(s)	968, 2195
BpmI	CTGGAG	1 cut(s)	2214
Bpu10I	CCTNAGC	2 cut(s)	1425, 1842
Bpu1102I	GCTNAGC	1 cut(s)	1869
BpuEI	CTTGAG	4 cut(s)	620, 852, 958, 1967
BpuMI	CCSGG	2 cut(s)	1221, 2475
Bsa29I	ATCGAT	1 cut(s)	2369
BsaJI	CCNNGG	4 cut(s)	1621, 1655, 2173, 2474
BsaXI	ACNNNNNCTCC	2 cut(s)	402, 432
Bsc4I	CCNNNNNNNGG	4 cut(s)	1642, 1730, 2331, 2389
Bse1I	ACTGG	3 cut(s)	416, 712, 821
Bse21I	CCTNAGG	1 cut(s)	2387
Bse3DI	GCAATG	2 cut(s)	1464, 1938
BseBI	CCWGG	3 cut(s)	2148, 2164, 2174
BseCI	ATCGAT	1 cut(s)	2369
BseDI	CCNNGG	4 cut(s)	1621, 1655, 2173, 2474
BseGI	GGATG	9 cut(s)	115, 199, 577, 655, 745, 961, 1384, 1669, 2104
BseLI	CCNNNNNNNGG	4 cut(s)	1642, 1730, 2331, 2389
BseMI	GCAATG	2 cut(s)	1464, 1938
BseMII	CTCAG	9 cut(s)	216, 542, 777, 1344, 1347, 1439, 1856, 1860, 2378
BseNI	ACTGG	3 cut(s)	416, 712, 821
BseRI	GAGGAG	7 cut(s)	89, 356, 855, 941, 1133, 1757, 2186
BseSI	GKGCMC	1 cut(s)	2395
BseXI	GCAGC	9 cut(s)	175, 178, 184, 187, 510, 1618, 1863, 1885, 2482
BsgI	GTGCAG	1 cut(s)	1539
BshFI	GGCC	4 cut(s)	1620, 1896, 2324, 2393
BshNI	GGYRCC	2 cut(s)	1904, 2401
BshVI	ATCGAT	1 cut(s)	2369
BsiHKCI	CYCGRG	1 cut(s)	1674
BsiSI	CCGG	2 cut(s)	1220, 2474
BslFI	GGGAC	3 cut(s)	1581, 2095, 2190
BslI	CCNNNNNNNGG	4 cut(s)	1642, 1730, 2331, 2389
BsmAI	GTCTC	1 cut(s)	1748
BsmFI	GGGAC	3 cut(s)	1581, 2095, 2190
BsmI	GAATGC	1 cut(s)	25
BsnI	GGCC	4 cut(s)	1620, 1896, 2324, 2393
BsoBI	CYCGRG	1 cut(s)	1674
Bsp120I	GGGCCC	1 cut(s)	2391
Bsp1286I	GDGCHC	1 cut(s)	2395
Bsp1407I	TGTACA	1 cut(s)	1851
Bsp143I	GATC	7 cut(s)	853, 1326, 1417, 2366, 2370, 2379, 2437
Bsp1720I	GCTNAGC	1 cut(s)	1869
BspACI	CCGC	5 cut(s)	647, 713, 1823, 1937, 2405
BspANI	GGCC	4 cut(s)	1620, 1896, 2324, 2393
BspCNI	CTCAG	9 cut(s)	217, 541, 778, 1343, 1348, 1438, 1855, 1861, 2379
BspDI	ATCGAT	1 cut(s)	2369
BspHI	TCATGA	1 cut(s)	2489
BspLI	GGNNCC	9 cut(s)	783, 1384, 1647, 1722, 1895, 1906, 2323, 2393, 2403
BspMI	ACCTGC	1 cut(s)	713
BspOI	GCTAGC	1 cut(s)	977
BspPI	GGATC	3 cut(s)	2365, 2374, 2374
BspQI	GCTCTTC	1 cut(s)	1473
BspT107I	GGYRCC	2 cut(s)	1904, 2401
BspTI	CTTAAG	1 cut(s)	770
BsrDI	GCAATG	2 cut(s)	1464, 1938
BsrGI	TGTACA	1 cut(s)	1851
BsrI	ACTGG	3 cut(s)	416, 712, 821
BssECI	CCNNGG	4 cut(s)	1621, 1655, 2173, 2474
BssMI	GATC	7 cut(s)	853, 1326, 1417, 2366, 2370, 2379, 2437
BssSI	CACGAG	2 cut(s)	1235, 1758
BssT1I	CCWWGG	2 cut(s)	1621, 1655
Bst2BI	CACGAG	2 cut(s)	1235, 1758
Bst2UI	CCWGG	3 cut(s)	2148, 2164, 2174
Bst4CI	ACNGT	6 cut(s)	967, 1054, 1159, 1216, 1922, 2113
Bst6I	CTCTTC	2 cut(s)	282, 1473
BstAFI	CTTAAG	1 cut(s)	770
BstAUI	TGTACA	1 cut(s)	1851
BstC8I	GCNNGC	4 cut(s)	975, 1554, 1618, 2456
BstF5I	GGATG	9 cut(s)	115, 199, 577, 655, 745, 961, 1384, 1669, 2104
BstH2I	RGCGCY	1 cut(s)	2212
BstHHI	GCGC	4 cut(s)	2024, 2211, 2301, 2486
BstKTI	GATC	7 cut(s)	856, 1329, 1420, 2369, 2373, 2382, 2440
BstMAI	GTCTC	1 cut(s)	1748
BstMBI	GATC	7 cut(s)	853, 1326, 1417, 2366, 2370, 2379, 2437
BstMWI	GCNNNNNNNGC	7 cut(s)	353, 719, 1480, 1613, 1708, 1822, 2296
BstNI	CCWGG	3 cut(s)	2148, 2164, 2174
BstNSI	RCATGY	3 cut(s)	1449, 2266, 2424
BstSCI	CCNGG	5 cut(s)	1219, 2146, 2162, 2172, 2473
BstSFI	CTRYAG	2 cut(s)	591, 828
BstSLI	GKGCMC	1 cut(s)	2395
BstV1I	GCAGC	9 cut(s)	175, 178, 184, 187, 510, 1618, 1863, 1885, 2482
BstV2I	GAAGAC	2 cut(s)	968, 2195
BstX2I	RGATCY	3 cut(s)	853, 1326, 2379
BstXI	CCANNNNNNTGG	1 cut(s)	509
BstYI	RGATCY	3 cut(s)	853, 1326, 2379
Bsu15I	ATCGAT	1 cut(s)	2369
Bsu36I	CCTNAGG	1 cut(s)	2387
BsuI	GTATCC	1 cut(s)	1042
BsuRI	GGCC	4 cut(s)	1620, 1896, 2324, 2393
BsuTUI	ATCGAT	1 cut(s)	2369
BtgZI	GCGATG	1 cut(s)	411
BtsCI	GGATG	9 cut(s)	115, 199, 577, 655, 745, 961, 1384, 1669, 2104
BtsIMutI	CAGTG	4 cut(s)	463, 828, 1059, 1212
BveI	ACCTGC	1 cut(s)	713
Cac8I	GCNNGC	4 cut(s)	975, 1554, 1618, 2456
CaiI	CAGNNNCTG	1 cut(s)	1169
CciI	TCATGA	1 cut(s)	2489
CfoI	GCGC	4 cut(s)	2024, 2211, 2301, 2486
Cfr13I	GGNCC	5 cut(s)	1720, 1894, 2322, 2391, 2392
ClaI	ATCGAT	1 cut(s)	2369
CseI	GACGC	1 cut(s)	122
Csp6I	GTAC	6 cut(s)	1664, 1686, 1852, 1983, 2109, 2129
CviQI	GTAC	6 cut(s)	1664, 1686, 1852, 1983, 2109, 2129
DpnI	GATC	7 cut(s)	855, 1328, 1419, 2368, 2372, 2381, 2439
DpnII	GATC	7 cut(s)	853, 1326, 1417, 2366, 2370, 2379, 2437
DraI	TTTAAA	1 cut(s)	1578
DrdI	GACNNNNNNGTC	1 cut(s)	2425
DseDI	GACNNNNNNGTC	1 cut(s)	2425
Eam1104I	CTCTTC	2 cut(s)	282, 1473
EarI	CTCTTC	2 cut(s)	282, 1473
EciI	GGCGGA	1 cut(s)	1952
Eco130I	CCWWGG	2 cut(s)	1621, 1655
Eco24I	GRGCYC	1 cut(s)	2395
Eco47I	GGWCC	1 cut(s)	1720
Eco47III	AGCGCT	1 cut(s)	2210
Eco57I	CTGAAG	9 cut(s)	54, 125, 138, 798, 870, 969, 1155, 1497, 1754
Eco81I	CCTNAGG	1 cut(s)	2387
Eco88I	CYCGRG	1 cut(s)	1674
EcoO109I	RGGNCCY	3 cut(s)	1720, 2322, 2391
EcoRI	GAATTC	1 cut(s)	931
EcoRII	CCWGG	3 cut(s)	2146, 2162, 2172
EcoT14I	CCWWGG	2 cut(s)	1621, 1655
EcoT38I	GRGCYC	1 cut(s)	2395
ErhI	CCWWGG	2 cut(s)	1621, 1655
FaqI	GGGAC	3 cut(s)	1581, 2095, 2190
FbaI	TGATCA	1 cut(s)	1417
FokI	GGATG	9 cut(s)	122, 206, 584, 662, 752, 968, 1391, 1656, 2111
FriOI	GRGCYC	1 cut(s)	2395
FspBI	CTAG	3 cut(s)	974, 1656, 2411
FspI	TGCGCA	1 cut(s)	2300
GlaI	GCGC	4 cut(s)	2023, 2210, 2300, 2485
GsuI	CTGGAG	1 cut(s)	2214
HaeII	RGCGCY	1 cut(s)	2212
HaeIII	GGCC	4 cut(s)	1620, 1896, 2324, 2393
HapII	CCGG	2 cut(s)	1220, 2474
HgaI	GACGC	1 cut(s)	122
HhaI	GCGC	4 cut(s)	2024, 2211, 2301, 2486
Hin6I	GCGC	4 cut(s)	2022, 2209, 2299, 2484
HinP1I	GCGC	4 cut(s)	2022, 2209, 2299, 2484
HindIII	AAGCTT	2 cut(s)	1004, 1766
HpaII	CCGG	2 cut(s)	1220, 2474
HphI	GGTGA	7 cut(s)	23, 221, 341, 371, 694, 1307, 1448
Hpy166II	GTNNAC	3 cut(s)	1456, 1531, 2058
Hpy8I	GTNNAC	3 cut(s)	1456, 1531, 2058
HpyAV	CCTTC	7 cut(s)	100, 361, 892, 1058, 1195, 1659, 2169
HpyCH4III	ACNGT	6 cut(s)	967, 1054, 1159, 1216, 1922, 2113
HpyCH4IV	ACGT	2 cut(s)	675, 1795
HpyF10VI	GCNNNNNNNGC	7 cut(s)	353, 719, 1480, 1613, 1708, 1822, 2296
HpySE526I	ACGT	2 cut(s)	675, 1795
HspAI	GCGC	4 cut(s)	2022, 2209, 2299, 2484
Ksp22I	TGATCA	1 cut(s)	1417
Kzo9I	GATC	7 cut(s)	853, 1326, 1417, 2366, 2370, 2379, 2437
LguI	GCTCTTC	1 cut(s)	1473
LmnI	GCTCC	4 cut(s)	174, 781, 970, 1382
Lsp1109I	GCAGC	9 cut(s)	175, 178, 184, 187, 510, 1618, 1863, 1885, 2482
LweI	GCATC	9 cut(s)	184, 751, 1066, 1078, 1156, 1240, 1678, 1961, 2056
MaeI	CTAG	3 cut(s)	974, 1656, 2411
MaeII	ACGT	2 cut(s)	675, 1795
MaeIII	GTNAC	6 cut(s)	523, 871, 1159, 1309, 1406, 2427
MalI	GATC	7 cut(s)	855, 1328, 1419, 2368, 2372, 2381, 2439
MboI	GATC	7 cut(s)	853, 1326, 1417, 2366, 2370, 2379, 2437
MfeI	CAATTG	2 cut(s)	294, 2343
MflI	RGATCY	3 cut(s)	853, 1326, 2379
MhlI	GDGCHC	1 cut(s)	2395
MlyI	GAGTC	8 cut(s)	245, 274, 1199, 1750, 1754, 1813, 2185, 2350
MmeI	TCCRAC	4 cut(s)	228, 388, 400, 2238
MroXI	GAANNNNTTC	2 cut(s)	1500, 1767
MseI	TTAA	7 cut(s)	132, 560, 771, 1577, 1728, 2124, 2205
MslI	CAYNNNNRTG	2 cut(s)	1469, 1526
MspA1I	CMGCKG	3 cut(s)	1279, 1616, 1876
MspCI	CTTAAG	1 cut(s)	770
MspI	CCGG	2 cut(s)	1220, 2474
MspR9I	CCNGG	5 cut(s)	1221, 2148, 2164, 2174, 2475
MunI	CAATTG	2 cut(s)	294, 2343
Mva1269I	GAATGC	1 cut(s)	25
MvaI	CCWGG	3 cut(s)	2148, 2164, 2174
MwoI	GCNNNNNNNGC	7 cut(s)	353, 719, 1480, 1613, 1708, 1822, 2296
NciI	CCSGG	2 cut(s)	1221, 2475
NdeII	GATC	7 cut(s)	853, 1326, 1417, 2366, 2370, 2379, 2437
NheI	GCTAGC	1 cut(s)	973
NlaIV	GGNNCC	9 cut(s)	783, 1384, 1647, 1722, 1895, 1906, 2323, 2393, 2403
NmuCI	GTSAC	2 cut(s)	1309, 2427
NsbI	TGCGCA	1 cut(s)	2300
NspI	RCATGY	3 cut(s)	1449, 2266, 2424
OliI	CACNNNNGTG	1 cut(s)	1526
PaeR7I	CTCGAG	1 cut(s)	1674
PagI	TCATGA	1 cut(s)	2489
PciI	ACATGT	1 cut(s)	2420
PciSI	GCTCTTC	1 cut(s)	1473
PctI	GAATGC	1 cut(s)	25
PdmI	GAANNNNTTC	2 cut(s)	1500, 1767
PfeI	GAWTC	8 cut(s)	632, 749, 860, 949, 1079, 1372, 1518, 1546
PleI	GAGTC	8 cut(s)	245, 273, 1199, 1750, 1753, 1813, 2185, 2350
PpsI	GAGTC	8 cut(s)	245, 273, 1199, 1750, 1753, 1813, 2185, 2350
PpuMI	RGGWCCY	1 cut(s)	1720
PscI	ACATGT	1 cut(s)	2420
Psp5II	RGGWCCY	1 cut(s)	1720
Psp6I	CCWGG	3 cut(s)	2146, 2162, 2172
PspGI	CCWGG	3 cut(s)	2146, 2162, 2172
PspN4I	GGNNCC	9 cut(s)	783, 1384, 1647, 1722, 1895, 1906, 2323, 2393, 2403
PspOMI	GGGCCC	1 cut(s)	2391
PspPI	GGNCC	5 cut(s)	1720, 1894, 2322, 2391, 2392
PspPPI	RGGWCCY	1 cut(s)	1720
PspXI	VCTCGAGB	1 cut(s)	1674
PstNI	CAGNNNCTG	1 cut(s)	1169
PsuI	RGATCY	3 cut(s)	853, 1326, 2379
PvuII	CAGCTG	3 cut(s)	1279, 1616, 1876
RsaI	GTAC	6 cut(s)	1665, 1687, 1853, 1984, 2110, 2130
RsaNI	GTAC	6 cut(s)	1664, 1686, 1852, 1983, 2109, 2129
RseI	CAYNNNNRTG	2 cut(s)	1469, 1526
SapI	GCTCTTC	1 cut(s)	1473
SaqAI	TTAA	7 cut(s)	132, 560, 771, 1577, 1728, 2124, 2205
Sau3AI	GATC	7 cut(s)	853, 1326, 1417, 2366, 2370, 2379, 2437
Sau96I	GGNCC	5 cut(s)	1720, 1894, 2322, 2391, 2392
SchI	GAGTC	8 cut(s)	245, 274, 1199, 1750, 1754, 1813, 2185, 2350
ScrFI	CCNGG	5 cut(s)	1221, 2148, 2164, 2174, 2475
SduI	GDGCHC	1 cut(s)	2395
SfaNI	GCATC	9 cut(s)	184, 751, 1066, 1078, 1156, 1240, 1678, 1961, 2056
SfcI	CTRYAG	2 cut(s)	591, 828
Sfr274I	CTCGAG	1 cut(s)	1674
SinI	GGWCC	1 cut(s)	1720
SlaI	CTCGAG	1 cut(s)	1674
SmiMI	CAYNNNNRTG	2 cut(s)	1469, 1526
SmlI	CTYRAG	6 cut(s)	599, 770, 867, 937, 1674, 1946
SmoI	CTYRAG	6 cut(s)	599, 770, 867, 937, 1674, 1946
SsiI	CCGC	5 cut(s)	647, 713, 1823, 1937, 2405
SspMI	CTAG	3 cut(s)	974, 1656, 2411
StyD4I	CCNGG	5 cut(s)	1219, 2146, 2162, 2172, 2473
StyI	CCWWGG	2 cut(s)	1621, 1655
TaaI	ACNGT	6 cut(s)	967, 1054, 1159, 1216, 1922, 2113
TaiI	ACGT	2 cut(s)	678, 1798
TaqI	TCGA	3 cut(s)	276, 1675, 2369
TatI	WGTACW	1 cut(s)	1851
TauI	GCSGC	1 cut(s)	2407
TfiI	GAWTC	8 cut(s)	632, 749, 860, 949, 1079, 1372, 1518, 1546
Tru1I	TTAA	7 cut(s)	132, 560, 771, 1577, 1728, 2124, 2205
Tru9I	TTAA	7 cut(s)	132, 560, 771, 1577, 1728, 2124, 2205
TscAI	CASTG	4 cut(s)	463, 828, 1059, 1219
TseFI	GTSAC	2 cut(s)	1309, 2427
TseI	GCWGC	9 cut(s)	163, 166, 197, 200, 498, 1631, 1873, 1876, 2470
Tsp45I	GTSAC	2 cut(s)	1309, 2427
TspDTI	ATGAA	6 cut(s)	623, 849, 1254, 1560, 1977, 2061
TspRI	CASTG	4 cut(s)	463, 828, 1059, 1219
Vha464I	CTTAAG	1 cut(s)	770
VpaK11BI	GGWCC	1 cut(s)	1720
XapI	RAATTY	5 cut(s)	157, 878, 931, 1579, 1787
XceI	RCATGY	3 cut(s)	1449, 2266, 2424
XhoI	CTCGAG	1 cut(s)	1674
XmaJI	CCTAGG	1 cut(s)	1655
XmnI	GAANNNNTTC	2 cut(s)	1500, 1767
XspI	CTAG	3 cut(s)	974, 1656, 2411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.