Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007786

Physical Location & Seq

Genomic Coordinates Reverse (-)

19756315 .. 19761736

Gene Structure 5422 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1120800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3852 bp

ATGGAAAATGGTGATAAGATTGCTGGTGGGTCTGAAGTGGGGGAGAACAAGGGTGTGGATGTTAAGGTTGTTGAGGAGAGGGTTGTAGAGGGGTCTAATGGACTCAAGGATGATGCGGAAGATGAGGTTTTCGAGGAGGCGATAGAGACGCAGGAGAATTCGCAGGAGCAGGGCAGTAAAGTTGATTTAGTGGATGCTGTTGTTGTTGGTGAAGAAAGAGAGACTGAGACGGTTGGTGGCTTGGGGTTGGCGTCTCTTATTGAGAGTCCTAGTGTCGATACATTTGAAGAGGCAATTGAAGTTCCTGATGAGGTTGGGAAATCTGATGATGATGATGCTGAAGTGAAGGTGGAAAATCTTGTAGGTGGGAACAGCGATGATGAAGTTGGAGTGGCAGGCGGAATTGATGATGAACAGACTAAAAAGGAAGCAGTCACTGAGGAAACAAATGGATTGACAGATGATGGGTTGGTGGGCAGCCAAGAAGATGCAGTAAAAGAAGTTACTCAGGTTGAGGCTGGTGGAGGAATATCAAGTTTGACAGGTGGGGATGAGGCTGATGTAAAGTCTGTAGATCTCGAGAATGTGAAGTTTGAAAAAGACAATTTTGAATTAAATGGACTTGCAGATGGCTTGGTGGGGAGCCAAGACGTTGAGGTGAAAGAAGTATCTGAGATTATTACTGGTGCGGAAATAGTAGGTTTGACAAATGTGGGTGAGGTTGATTCAAAGCCTAATGTTGTTCTTGAAAATAAGGAGCCCGAGAAGGATGATCTTGATAATTCAACTTCAGAGCCAGTATCTACAGATGAAAAATTAGATACTGAAGATCTTGATTCTCCTCAAGTAACTGAATTTAATAAGGAGATTTTGAAGGAAGCTGGTAATGGTCAGGAGTTAGAGGAGAATTCCTTGAGCATAGAGAATCAGGATGAGAAGACTGTGGATCTACTAAGTGCTTCAGATGGCGTTCCTTTGAAGCTTGAAGATGATAACGGTGTGGAACTGCTTGATAGAAACATGGATACAGTGCATCAGGAAGGTGATAGTGCTGAATCAAATGATGCCACGCTTGGCATTGAAGAAAAGCAGGAGTATAATAAGACTGAAGAACTGAGAGATACTTTGACTTTTACAGATGCAGAACATCAAGGTTTCAGTAATGGAGAAGTGAAAGACTCTTTCACTGTGCTGGGTTCAGAGCATCACGAGGAAAAATCTGAACCAAAAAGCATTTCATCTGATAAACAACTGTCTGGGGAGGATAGTGAGGAAATGATTGTTACGTCAGAAAGGGAGATCTCAGCTCTTTCTGAAAGATCTGCAGCTGAGAAGACAGAGAAGATTCAGGATGGTGCCACTAATTTGAGAGCACAAAGTAACAAAGATGATCAACCTCAGCGAGCTGATGAAATTGCTCCTGAAGTTCGTGACAACATTGCTGTGCCTGAAGAGCCTGAAAAGAAAGAAAACATTCAGGCAGAAAAGGGAGTCACAAAAGTGAACAAAGAACAAGAGATTCAACCTGTATCAACTCTTTCTTCATCTGGAAATTCTACACAACCAAGTCCCCCTCCTGCTCGGCCAGCTGGCCTTGGGCGTGCTGCCCCACTATTGGAACCTGCGCCTAGGGTGGTCCAGCATCCTCGAGTAAATGGTACAGTATCTCATGCTCAAAACCAGCAAATTGAGGACCCCGTTAATGGAGAGACAGAGGAGTCTGATGAGACTCGTGAAAAGCTTCAGATGATAAGAGTGAAATTTTTACGTCTTGCACATAGACTTGGGCAGACTCCGCATAATGTTGTTGTGGCTCAGGTTTTGTACAGATTGGGATTAGCTGAGCAGCTGCGTGGTAGAAATGGGGGTCGTGTTGGTGCCTTTAGTTTTGACCGTGCGAGTGCCATGGCTGAACAGCTTGAGGCATCAGGGAATGAACCCCTTGATTTTGCTTGTACCATTATGGTTCTTGGAAAGTCAGGAGTTGGTAAAAGTGCAACCATCAATTCTATATTTGATGAAAAGAGGTTCACCACTGATGCTTTTCAGATGGGGACAAAGAAAGTTCAAGATGTTGTGGGTACTGTGCAAGGGATTAAGGTACGGGTCATTGACACACCTGGACTTTTACCTTCCTGGTCGGACCAGCGACAGAATGAGAAGACTCTCCTAAATGTTAAGCGCTTTATCAAGAAAACACCTCCAGATATTGTGTTGTATCTTGATAGGTTGGACATGCAGAGCAGGGATTTCAGTGATATGCCACTCTTGCGCACCATCACAGACATTTTTGGGCCCTCTATATGGTTCAATGCAATTGTGGTTCTGACTCATGCTGGATCAGCTCCTCCTGAGGGCCCAAATGGTGCCGCTTCTAGTTATGACATGTTTGTCACTTCACGATCTCATGTTGTTCAGCAAGCCATTCGTCAGGCAGCTGGGGATATGCGCCTCATGAATCCTGTTTCATTAGTAGAGAACCACTCTGCTTGCAGAATTAATAGGGCTGGGCAAAGAGTCCTGCCAAATGGTCAGGTTTGGAAACCTCATTTGTTATTGCTCTCCTTTGCATCCAAGATTCTGGCCGAAGCAAATGCATTATTGAAGTTGCAAGATAGTCCACCTGGAAAACCTTTTGCGACTCGAACTAGGGCACCTCCTTTACCTTTCTTGCTTTCATCACTCCTTCAGTCAAGACCACAGTTGAAGCTGCCTGAGGAGCAGTTTGGTGATGATGACAGTCTAGACGATGATCTGGATGAATCTTCAGATTCTGATGATGAATCAGAATTTGATGAATTGCCCCCATTCAGACGTTTGACCAAGGCCCAGGTGGAAAAACTCTCAAAAGCACAGAAAAAGGCATATTTTGATGAGTTAGAGTACAGGGAAAAGCTCTTTATGAAGAAACAGTTGAAGGAGGAGAAAAAGCGACGGAAGTTGATGAAGAAAATGGCAGCTGCATCAAACGAACTGCCAAGTGATTATGTCGAAAATGTAGAAGAAGACAGTTCTGGTGCAGCATCTGTACCTATTCCCATGCCAGATTTGGCATTGCCGGCTTCCTTTGATTCTGATAATCCTACTCACCGTTACCGGTATCTAGATTCCTCCAACCAGTGGCTCGTAAGACCTGTTCTGGAACAGCATGGTTGGGATCATGATGTTGGTTATGAGGGTATAAATGCAGAAAGACTGTTTGTTGTTAAAGAAAAGATACCCTTATCTTTTTCTGGCCAGGTTACCAAGGATAAGAAGGACGCCAATGTCCAAATGGAAATTGCTACTTCAATAAAGCATGGAGAAGGGAAAGCGACTTCATTAGGGTTTGATATGCAGACTGTTGGAAAGGACTTGGCTTATACTCTACGAAGTGACACAAGGATCAGTAACTTTAGGAAGAATAAGGCTACTGCTGGTCTCTCAGTTACTCTCCTGGGTGATGCTGTATCGGCTGGTATGAAAGTTGAAGACAAATTTATTGCTAATAAACGGTTCCAATTGGTTATGACTGGTGGTGCAATGACTGCGCGTGGTGACGTTGCTTATGGTGGTAGTTTGGAGGCTCAGTTGAGAGACAAAGACCATCCTTTGGGTCGTTCCCTATCAACCCTTGGGCTCTCTGTCATGGATTGGCATGGAGATCTTGCCATAGGGTGCAATATACAGTCTCAGATTCCAGTTGGACGGCATACAAACCTGATTGCTCGTGCCAATTTGAACAATAGAGGGGCAGGACAACTCAGCTTGCGCTTAAATAGCTCAGAACAGCTTCAATTAGCATTGATCGGCCTCGTTCCTCTGCTCAGAAAGTTGTTCACTTTTCCTCAACAGTTGCAGTATGGGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006605 GO:0006810 GO:0006886 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0019867 GO:0019899 GO:0023052 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0033036 GO:0033365 GO:0034613 GO:0038023 GO:0042170 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051087 GO:0051117 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060089 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AIG1 TOC159_MAD

Protein Analysis

1284

Amino Acids

140.28

Weight (kDa)

4.64

Isoelectric Point (pI)

41.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AIG1	PF04548	652 - 798	1.2e-25	AIG1 family
MMR_HSR1	PF01926	654 - 740	1.5e-09	50S ribosome-binding GTPase
TOC159_MAD	PF11886	1010 - 1273	3.3e-126	Translocase of chloroplast 159/132, membrane anchor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007786)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16640 AT2G16640 AT2G16640 AT3G16620
fragaria_vesca	FvH4_2g01650 FvH4_2g01650
malus_domestica	MD05G1117800.v1.1 MD10G1120800.v1.1
prunus_persica	Prupe.8G162300_v2.0.a1 Prupe.8G162300_v2.0.a1
pyrus_communis	pycom05g11180 pycom10g10400
rosa_chinensis	RchiOBHm_Chr6g0244541
rosa_laevigata	RLG00000015350
rosa_multiflora	Rmu_co8516985.1_g000001 Rmu_sc0003679.1_g000009 Rmu_sc0017888.1_g000001
rosa_roxburghii	Rroxscaffold_7G00215770
rosa_rugosa	Rorug05G0509200
rosa_samantha	Rh6AG024900 Rh6BG019800 Rh6CG017300 Rh6DG018300
rosa_wichuraiana	Rw6G002030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	1717, 2390, 3266
Acc16I	TGCGCA	1 cut(s)	2273
Acc36I	ACCTGC	1 cut(s)	1630
AccB1I	GGYRCC	4 cut(s)	1355, 1877, 2366, 2653
AccB7I	CCANNNNNTGG	2 cut(s)	3120, 3592
AccII	CGCG	1 cut(s)	3532
AciI	CCGC	5 cut(s)	116, 399, 689, 1796, 2370
AclWI	GGATC	4 cut(s)	954, 2347, 3165, 3392
AcoI	YGGCCR	3 cut(s)	1583, 2583, 3235
AcsI	RAATTY	7 cut(s)	157, 854, 907, 1552, 1760, 2789, 3476
AcyI	GRCGYC	2 cut(s)	251, 3261
AfaI	GTAC	7 cut(s)	1660, 1826, 1957, 2083, 2103, 2885, 3030
AfeI	AGCGCT	1 cut(s)	2183
AfiI	CCNNNNNNNGG	6 cut(s)	1615, 1703, 2304, 2354, 3120, 3592
AflIII	ACRYGT	1 cut(s)	2385
AgeI	ACCGGT	1 cut(s)	3096
AjnI	CCWGG	6 cut(s)	2119, 2135, 2623, 2829, 3237, 3435
AjuI	GAANNNNNNNTTGG	2 cut(s)	1219, 1251
AleI	CACNNNNGTG	1 cut(s)	1499
Alw21I	GWGCWC	1 cut(s)	1375
Alw26I	GTCTC	9 cut(s)	140, 215, 221, 258, 1703, 1721, 3425, 3570, 3675
AlwI	GGATC	4 cut(s)	954, 2347, 3165, 3392
AlwNI	CAGNNNCTG	3 cut(s)	437, 2774, 3026
Ama87I	CYCGRG	3 cut(s)	578, 761, 1647
Aor51HI	AGCGCT	1 cut(s)	2183
AoxI	GGCC	8 cut(s)	1583, 1591, 2294, 2356, 2583, 2826, 3235, 3790
ApaI	GGGCCC	2 cut(s)	2298, 2360
ApoI	RAATTY	7 cut(s)	157, 854, 907, 1552, 1760, 2789, 3476
AseI	ATTAAT	1 cut(s)	2499
AsiGI	ACCGGT	1 cut(s)	3096
Asp700I	GAANNNNTTC	3 cut(s)	1473, 1740, 3771
AspA2I	CCTAGG	1 cut(s)	1628
AspLEI	GCGC	6 cut(s)	1627, 2184, 2274, 2451, 3532, 3753
AspS9I	GGNCC	8 cut(s)	1636, 1693, 2143, 2294, 2295, 2356, 2357, 2827
AvaI	CYCGRG	3 cut(s)	578, 761, 1647
AvaII	GGWCC	3 cut(s)	1636, 1693, 2143
AvrII	CCTAGG	1 cut(s)	1628
AxyI	CCTNAGG	2 cut(s)	2352, 2715
BaeGI	GKGCMC	3 cut(s)	2298, 2360, 2656
BalI	TGGCCA	1 cut(s)	3237
BanI	GGYRCC	4 cut(s)	1355, 1877, 2366, 2653
BanII	GRGCYC	4 cut(s)	762, 2298, 2360, 3621
BauI	CACGAG	3 cut(s)	1208, 1731, 3708
BbsI	GAAGAC	5 cut(s)	944, 1340, 2168, 3012, 3477
Bbv12I	GWGCWC	1 cut(s)	1375
BbvCI	CCTCAGC	1 cut(s)	1398
BccI	CCATC	8 cut(s)	458, 623, 959, 1346, 2009, 2044, 2285, 3594
BceAI	ACGGC	1 cut(s)	3704
BcgI	CGANNNNNNTGC	4 cut(s)	2408, 2442, 2576, 2610
BciT130I	CCWGG	6 cut(s)	2121, 2137, 2625, 2831, 3239, 3437
BciVI	GTATCC	1 cut(s)	1018
BclI	TGATCA	1 cut(s)	1390
BcoDI	GTCTC	9 cut(s)	140, 215, 221, 258, 1703, 1721, 3425, 3570, 3675
BfaI	CTAG	6 cut(s)	270, 1629, 2376, 2649, 2744, 3104
BfmI	CTRYAG	3 cut(s)	570, 804, 1323
BfoI	RGCGCY	1 cut(s)	2185
BfuAI	ACCTGC	1 cut(s)	1630
BfuI	GTATCC	1 cut(s)	1018
BglII	AGATCT	5 cut(s)	574, 829, 1299, 1319, 3643
BlnI	CCTAGG	1 cut(s)	1628
BlpI	GCTNAGC	1 cut(s)	1842
Bme1390I	CCNGG	6 cut(s)	2121, 2137, 2625, 2831, 3239, 3437
Bme18I	GGWCC	3 cut(s)	1636, 1693, 2143
BmeT110I	CYCGRG	3 cut(s)	578, 761, 1647
BmgT120I	GGNCC	8 cut(s)	1636, 1693, 2143, 2294, 2295, 2356, 2357, 2827
BmrFI	CCNGG	6 cut(s)	2121, 2137, 2625, 2831, 3239, 3437
BpiI	GAAGAC	5 cut(s)	944, 1340, 2168, 3012, 3477
BplI	GAGNNNNNCTC	2 cut(s)	2468, 2500
BpmI	CTGGAG	1 cut(s)	2187
Bpu10I	CCTNAGC	2 cut(s)	1398, 1815
Bpu1102I	GCTNAGC	1 cut(s)	1842
BpuEI	CTTGAG	4 cut(s)	89, 828, 934, 1940
BsaHI	GRCGYC	2 cut(s)	251, 3261
BsaI	GGTCTC	1 cut(s)	3425
BsaJI	CCNNGG	8 cut(s)	1594, 1628, 1905, 2823, 2829, 3246, 3436, 3613
BsaWI	WCCGGW	1 cut(s)	3096
BsaXI	ACNNNNNCTCC	2 cut(s)	3554, 3584
Bsc4I	CCNNNNNNNGG	6 cut(s)	1615, 1703, 2304, 2354, 3120, 3592
Bse118I	RCCGGY	2 cut(s)	3058, 3096
Bse1I	ACTGG	5 cut(s)	688, 797, 3118, 3517, 3680
Bse21I	CCTNAGG	2 cut(s)	2352, 2715
Bse3DI	GCAATG	3 cut(s)	1437, 3053, 3528
BseBI	CCWGG	6 cut(s)	2121, 2137, 2625, 2831, 3239, 3437
BseDI	CCNNGG	8 cut(s)	1594, 1628, 1905, 2823, 2829, 3246, 3436, 3613
BseLI	CCNNNNNNNGG	6 cut(s)	1615, 1703, 2304, 2354, 3120, 3592
BseMI	GCAATG	3 cut(s)	1437, 3053, 3528
BseNI	ACTGG	5 cut(s)	688, 797, 3118, 3517, 3680
BseRI	GAGGAG	8 cut(s)	89, 149, 831, 917, 1730, 2337, 2732, 2936
BseSI	GKGCMC	3 cut(s)	2298, 2360, 2656
BseYI	CCCAGC	3 cut(s)	1192, 2438, 2507
BsgI	GTGCAG	1 cut(s)	3039
Bsh1236I	CGCG	1 cut(s)	3532
BshFI	GGCC	8 cut(s)	1585, 1593, 2296, 2358, 2585, 2828, 3237, 3792
BshNI	GGYRCC	4 cut(s)	1355, 1877, 2366, 2653
BshTI	ACCGGT	1 cut(s)	3096
BsiHKAI	GWGCWC	1 cut(s)	1375
BsiHKCI	CYCGRG	3 cut(s)	578, 761, 1647
BsiSI	CCGG	2 cut(s)	3059, 3097
BslFI	GGGAC	2 cut(s)	1554, 2068
BslI	CCNNNNNNNGG	6 cut(s)	1615, 1703, 2304, 2354, 3120, 3592
BsmAI	GTCTC	9 cut(s)	140, 215, 221, 258, 1703, 1721, 3425, 3570, 3675
BsmBI	CGTCTC	3 cut(s)	140, 221, 258
BsmFI	GGGAC	2 cut(s)	1554, 2068
BsnI	GGCC	8 cut(s)	1585, 1593, 2296, 2358, 2585, 2828, 3237, 3792
Bso31I	GGTCTC	1 cut(s)	3425
BsoBI	CYCGRG	3 cut(s)	578, 761, 1647
Bsp120I	GGGCCC	2 cut(s)	2294, 2356
Bsp1286I	GDGCHC	6 cut(s)	762, 1375, 2298, 2360, 2656, 3621
Bsp1407I	TGTACA	1 cut(s)	1824
Bsp1720I	GCTNAGC	1 cut(s)	1842
Bsp19I	CCATGG	1 cut(s)	1905
BspACI	CCGC	5 cut(s)	116, 399, 689, 1796, 2370
BspANI	GGCC	8 cut(s)	1585, 1593, 2296, 2358, 2585, 2828, 3237, 3792
BspFNI	CGCG	1 cut(s)	3532
BspHI	TCATGA	2 cut(s)	2454, 3160
BspMAI	CTGCAG	1 cut(s)	1327
BspMI	ACCTGC	1 cut(s)	1630
BspPI	GGATC	4 cut(s)	954, 2347, 3165, 3392
BspQI	GCTCTTC	1 cut(s)	1446
BspT107I	GGYRCC	4 cut(s)	1355, 1877, 2366, 2653
BspTNI	GGTCTC	1 cut(s)	3425
BsrDI	GCAATG	3 cut(s)	1437, 3053, 3528
BsrFI	RCCGGY	2 cut(s)	3058, 3096
BsrGI	TGTACA	1 cut(s)	1824
BsrI	ACTGG	5 cut(s)	688, 797, 3118, 3517, 3680
BssAI	RCCGGY	2 cut(s)	3058, 3096
BssECI	CCNNGG	8 cut(s)	1594, 1628, 1905, 2823, 2829, 3246, 3436, 3613
BssNI	GRCGYC	2 cut(s)	251, 3261
BssSI	CACGAG	3 cut(s)	1208, 1731, 3708
BssT1I	CCWWGG	6 cut(s)	1594, 1628, 1905, 2823, 3246, 3613
Bst2BI	CACGAG	3 cut(s)	1208, 1731, 3708
Bst2UI	CCWGG	6 cut(s)	2121, 2137, 2625, 2831, 3239, 3437
Bst6I	CTCTTC	2 cut(s)	282, 1446
BstACI	GRCGYC	2 cut(s)	251, 3261
BstAPI	GCANNNNNTGC	1 cut(s)	3527
BstAUI	TGTACA	1 cut(s)	1824
BstC8I	GCNNGC	9 cut(s)	397, 1404, 1587, 1591, 1602, 2421, 2491, 3060, 3749
BstDSI	CCRYGG	1 cut(s)	1905
BstEII	GGTNACC	1 cut(s)	3241
BstFNI	CGCG	1 cut(s)	3532
BstH2I	RGCGCY	1 cut(s)	2185
BstHHI	GCGC	6 cut(s)	1627, 2184, 2274, 2451, 3532, 3753
BstMAI	GTCTC	9 cut(s)	140, 215, 221, 258, 1703, 1721, 3425, 3570, 3675
BstNI	CCWGG	6 cut(s)	2121, 2137, 2625, 2831, 3239, 3437
BstNSI	RCATGY	2 cut(s)	2239, 2389
BstPI	GGTNACC	1 cut(s)	3241
BstSCI	CCNGG	6 cut(s)	2119, 2135, 2623, 2829, 3237, 3435
BstSFI	CTRYAG	3 cut(s)	570, 804, 1323
BstSLI	GKGCMC	3 cut(s)	2298, 2360, 2656
BstUI	CGCG	1 cut(s)	3532
BstV2I	GAAGAC	5 cut(s)	944, 1340, 2168, 3012, 3477
BstX2I	RGATCY	6 cut(s)	574, 829, 946, 1299, 1319, 3643
BstXI	CCANNNNNNTGG	1 cut(s)	2581
BstYI	RGATCY	6 cut(s)	574, 829, 946, 1299, 1319, 3643
Bsu36I	CCTNAGG	2 cut(s)	2352, 2715
BsuI	GTATCC	1 cut(s)	1018
BsuRI	GGCC	8 cut(s)	1585, 1593, 2296, 2358, 2585, 2828, 3237, 3792
BtgI	CCRYGG	1 cut(s)	1905
BtgZI	GCGATG	1 cut(s)	390
BtsIMutI	CAGTG	6 cut(s)	435, 1035, 1185, 2034, 2260, 3125
BveI	ACCTGC	1 cut(s)	1630
Cac8I	GCNNGC	9 cut(s)	397, 1404, 1587, 1591, 1602, 2421, 2491, 3060, 3749
CaiI	CAGNNNCTG	3 cut(s)	437, 2774, 3026
CciI	TCATGA	2 cut(s)	2454, 3160
CfoI	GCGC	6 cut(s)	1627, 2184, 2274, 2451, 3532, 3753
Cfr10I	RCCGGY	2 cut(s)	3058, 3096
Cfr13I	GGNCC	8 cut(s)	1636, 1693, 2143, 2294, 2295, 2356, 2357, 2827
CseI	GACGC	3 cut(s)	157, 240, 3269
Csp6I	GTAC	7 cut(s)	1659, 1825, 1956, 2082, 2102, 2884, 3029
CspAI	ACCGGT	1 cut(s)	3096
CspCI	CAANNNNNGTGG	2 cut(s)	2471, 2506
CviQI	GTAC	7 cut(s)	1659, 1825, 1956, 2082, 2102, 2884, 3029
DrdI	GACNNNNNNGTC	3 cut(s)	1717, 2390, 3266
DseDI	GACNNNNNNGTC	3 cut(s)	1717, 2390, 3266
EaeI	YGGCCR	3 cut(s)	1583, 2583, 3235
Eam1104I	CTCTTC	2 cut(s)	282, 1446
EarI	CTCTTC	2 cut(s)	282, 1446
EciI	GGCGGA	1 cut(s)	414
Eco130I	CCWWGG	6 cut(s)	1594, 1628, 1905, 2823, 3246, 3613
Eco24I	GRGCYC	4 cut(s)	762, 2298, 2360, 3621
Eco31I	GGTCTC	1 cut(s)	3425
Eco47I	GGWCC	3 cut(s)	1636, 1693, 2143
Eco47III	AGCGCT	1 cut(s)	2183
Eco81I	CCTNAGG	2 cut(s)	2352, 2715
Eco88I	CYCGRG	3 cut(s)	578, 761, 1647
Eco91I	GGTNACC	1 cut(s)	3241
EcoO109I	RGGNCCY	3 cut(s)	1693, 2295, 2356
EcoO65I	GGTNACC	1 cut(s)	3241
EcoRI	GAATTC	2 cut(s)	157, 907
EcoRII	CCWGG	6 cut(s)	2119, 2135, 2623, 2829, 3237, 3435
EcoT14I	CCWWGG	6 cut(s)	1594, 1628, 1905, 2823, 3246, 3613
EcoT22I	ATGCAT	1 cut(s)	2599
EcoT38I	GRGCYC	4 cut(s)	762, 2298, 2360, 3621
ErhI	CCWWGG	6 cut(s)	1594, 1628, 1905, 2823, 3246, 3613
Esp3I	CGTCTC	3 cut(s)	140, 221, 258
FalI	AAGNNNNNCTT	2 cut(s)	3206, 3238
FaqI	GGGAC	2 cut(s)	1554, 2068
FbaI	TGATCA	1 cut(s)	1390
FriOI	GRGCYC	4 cut(s)	762, 2298, 2360, 3621
FspBI	CTAG	6 cut(s)	270, 1629, 2376, 2649, 2744, 3104
FspI	TGCGCA	1 cut(s)	2273
GlaI	GCGC	6 cut(s)	1626, 2183, 2273, 2450, 3531, 3752
GsaI	CCCAGC	3 cut(s)	1196, 2442, 2511
GsuI	CTGGAG	1 cut(s)	2187
HaeII	RGCGCY	1 cut(s)	2185
HaeIII	GGCC	8 cut(s)	1585, 1593, 2296, 2358, 2585, 2828, 3237, 3792
HapII	CCGG	2 cut(s)	3059, 3097
HgaI	GACGC	3 cut(s)	157, 240, 3269
HhaI	GCGC	6 cut(s)	1627, 2184, 2274, 2451, 3532, 3753
Hin1I	GRCGYC	2 cut(s)	251, 3261
Hin6I	GCGC	6 cut(s)	1625, 2182, 2272, 2449, 3530, 3751
HinP1I	GCGC	6 cut(s)	1625, 2182, 2272, 2449, 3530, 3751
HindIII	AAGCTT	2 cut(s)	980, 1739
HpaII	CCGG	2 cut(s)	3059, 3097
Hpy166II	GTNNAC	4 cut(s)	1504, 2031, 2621, 3819
Hpy8I	GTNNAC	4 cut(s)	1504, 2031, 2621, 3819
Hpy99I	CGWCG	1 cut(s)	2937
HpyAV	CCTTC	9 cut(s)	340, 760, 868, 1034, 2142, 2696, 2911, 3250, 3299
HpyCH4IV	ACGT	5 cut(s)	651, 1286, 1768, 2815, 3540
HpySE526I	ACGT	5 cut(s)	651, 1286, 1768, 2815, 3540
Hsp92I	GRCGYC	2 cut(s)	251, 3261
HspAI	GCGC	6 cut(s)	1625, 2182, 2272, 2449, 3530, 3751
KroI	GCCGGC	1 cut(s)	3058
KroNI	GCCGGC	1 cut(s)	3060
Ksp22I	TGATCA	1 cut(s)	1390
LguI	GCTCTTC	1 cut(s)	1446
LmnI	GCTCC	6 cut(s)	166, 642, 757, 1423, 2350, 2719
MaeI	CTAG	6 cut(s)	270, 1629, 2376, 2649, 2744, 3104
MaeII	ACGT	5 cut(s)	651, 1286, 1768, 2815, 3540
MfeI	CAATTG	3 cut(s)	294, 2316, 3500
MflI	RGATCY	6 cut(s)	574, 829, 946, 1299, 1319, 3643
MhlI	GDGCHC	6 cut(s)	762, 1375, 2298, 2360, 2656, 3621
MlsI	TGGCCA	1 cut(s)	3237
MluNI	TGGCCA	1 cut(s)	3237
MmeI	TCCRAC	6 cut(s)	367, 2121, 2211, 3138, 3325, 3664
Mox20I	TGGCCA	1 cut(s)	3237
Mph1103I	ATGCAT	1 cut(s)	2599
MroNI	GCCGGC	1 cut(s)	3058
MroXI	GAANNNNTTC	3 cut(s)	1473, 1740, 3771
MscI	TGGCCA	1 cut(s)	3237
MseI	TTAA	9 cut(s)	63, 614, 858, 1701, 2097, 2178, 2499, 3207, 3755
MslI	CAYNNNNRTG	2 cut(s)	1442, 1499
Msp20I	TGGCCA	1 cut(s)	3237
MspA1I	CMGCKG	5 cut(s)	1328, 1589, 1849, 2438, 2960
MspI	CCGG	2 cut(s)	3059, 3097
MspR9I	CCNGG	6 cut(s)	2121, 2137, 2625, 2831, 3239, 3437
MunI	CAATTG	3 cut(s)	294, 2316, 3500
MvaI	CCWGG	6 cut(s)	2121, 2137, 2625, 2831, 3239, 3437
MvnI	CGCG	1 cut(s)	3532
NaeI	GCCGGC	1 cut(s)	3060
NcoI	CCATGG	1 cut(s)	1905
NgoMIV	GCCGGC	1 cut(s)	3058
NmeAIII	GCCGAG	1 cut(s)	1561
NmuCI	GTSAC	6 cut(s)	433, 1430, 1492, 2392, 3374, 3536
NsbI	TGCGCA	1 cut(s)	2273
NsiI	ATGCAT	1 cut(s)	2599
NspI	RCATGY	2 cut(s)	2239, 2389
OliI	CACNNNNGTG	1 cut(s)	1499
PaeR7I	CTCGAG	2 cut(s)	578, 1647
PagI	TCATGA	2 cut(s)	2454, 3160
PciI	ACATGT	1 cut(s)	2385
PciSI	GCTCTTC	1 cut(s)	1446
PdiI	GCCGGC	1 cut(s)	3060
PdmI	GAANNNNTTC	3 cut(s)	1473, 1740, 3771
PflMI	CCANNNNNTGG	2 cut(s)	3120, 3592
PinAI	ACCGGT	1 cut(s)	3096
PpuMI	RGGWCCY	1 cut(s)	1693
PscI	ACATGT	1 cut(s)	2385
PshBI	ATTAAT	1 cut(s)	2499
Psp5II	RGGWCCY	1 cut(s)	1693
Psp6I	CCWGG	6 cut(s)	2119, 2135, 2623, 2829, 3237, 3435
PspEI	GGTNACC	1 cut(s)	3241
PspFI	CCCAGC	3 cut(s)	1192, 2438, 2507
PspGI	CCWGG	6 cut(s)	2119, 2135, 2623, 2829, 3237, 3435
PspOMI	GGGCCC	2 cut(s)	2294, 2356
PspPI	GGNCC	8 cut(s)	1636, 1693, 2143, 2294, 2295, 2356, 2357, 2827
PspPPI	RGGWCCY	1 cut(s)	1693
PspXI	VCTCGAGB	1 cut(s)	1647
PsrI	GAACNNNNNNTAC	2 cut(s)	3120, 3152
PstI	CTGCAG	1 cut(s)	1327
PstNI	CAGNNNCTG	3 cut(s)	437, 2774, 3026
PsuI	RGATCY	6 cut(s)	574, 829, 946, 1299, 1319, 3643
PvuII	CAGCTG	5 cut(s)	1328, 1589, 1849, 2438, 2960
RsaI	GTAC	7 cut(s)	1660, 1826, 1957, 2083, 2103, 2885, 3030
RsaNI	GTAC	7 cut(s)	1659, 1825, 1956, 2082, 2102, 2884, 3029
RseI	CAYNNNNRTG	2 cut(s)	1442, 1499
SapI	GCTCTTC	1 cut(s)	1446
SaqAI	TTAA	9 cut(s)	63, 614, 858, 1701, 2097, 2178, 2499, 3207, 3755
Sau96I	GGNCC	8 cut(s)	1636, 1693, 2143, 2294, 2295, 2356, 2357, 2827
ScrFI	CCNGG	6 cut(s)	2121, 2137, 2625, 2831, 3239, 3437
SduI	GDGCHC	6 cut(s)	762, 1375, 2298, 2360, 2656, 3621
SfcI	CTRYAG	3 cut(s)	570, 804, 1323
Sfr274I	CTCGAG	2 cut(s)	578, 1647
SinI	GGWCC	3 cut(s)	1636, 1693, 2143
SlaI	CTCGAG	2 cut(s)	578, 1647
SmiMI	CAYNNNNRTG	2 cut(s)	1442, 1499
SmlI	CTYRAG	6 cut(s)	104, 578, 843, 913, 1647, 1919
SmoI	CTYRAG	6 cut(s)	104, 578, 843, 913, 1647, 1919
SsiI	CCGC	5 cut(s)	116, 399, 689, 1796, 2370
SspMI	CTAG	6 cut(s)	270, 1629, 2376, 2649, 2744, 3104
StyD4I	CCNGG	6 cut(s)	2119, 2135, 2623, 2829, 3237, 3435
StyI	CCWWGG	6 cut(s)	1594, 1628, 1905, 2823, 3246, 3613
TaiI	ACGT	5 cut(s)	654, 1289, 1771, 2818, 3543
TaqI	TCGA	6 cut(s)	132, 276, 579, 1648, 2644, 2991
TatI	WGTACW	2 cut(s)	1824, 2883
TauI	GCSGC	1 cut(s)	2372
Tru1I	TTAA	9 cut(s)	63, 614, 858, 1701, 2097, 2178, 2499, 3207, 3755
Tru9I	TTAA	9 cut(s)	63, 614, 858, 1701, 2097, 2178, 2499, 3207, 3755
TscAI	CASTG	6 cut(s)	442, 1035, 1192, 2041, 2260, 3125
TseFI	GTSAC	6 cut(s)	433, 1430, 1492, 2392, 3374, 3536
Tsp45I	GTSAC	6 cut(s)	433, 1430, 1492, 2392, 3374, 3536
TspGWI	ACGGA	1 cut(s)	2950
TspRI	CASTG	6 cut(s)	442, 1035, 1192, 2041, 2260, 3125
Van91I	CCANNNNNTGG	2 cut(s)	3120, 3592
VpaK11BI	GGWCC	3 cut(s)	1636, 1693, 2143
VspI	ATTAAT	1 cut(s)	2499
XapI	RAATTY	7 cut(s)	157, 854, 907, 1552, 1760, 2789, 3476
XbaI	TCTAGA	2 cut(s)	2743, 3103
XceI	RCATGY	2 cut(s)	2239, 2389
XcmI	CCANNNNNNNNNTGG	3 cut(s)	2830, 3046, 3271
XhoI	CTCGAG	2 cut(s)	578, 1647
XmaJI	CCTAGG	1 cut(s)	1628
XmnI	GAANNNNTTC	3 cut(s)	1473, 1740, 3771
XspI	CTAG	6 cut(s)	270, 1629, 2376, 2649, 2744, 3104
Zsp2I	ATGCAT	1 cut(s)	2599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.