MD05G1166700.v1.1

Protein of unknown function (DUF760)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010353
Physical Location & Seq
Forward (+)
29697653 .. 29700091
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1166700.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGGACCATAGTCTCTCCCCGCTCTCAACCCTCAAGCTCTGCATAACCCCCGCCTTCGCCCCGCCCCGCTCCGCCGTCCAATCGCTGACCGCAAATCGGTTCTTCAGGCCGTTGACCGTCGTCGCCGGAGCCGGGGCGAGCCACTGCGAGTTCAGCAGCCTCAACTCGCCGCTCGACCCGCGGACCCGACCCGGAAAGGACCTCTGCACCGTCTTGCAGAACCATCCGCGGCTGTTCCACCTTTCCGTGGCTCAGGAGCTGAAGAAGTTGGCCGATGATCGGGAACTCGCTCTCTCTCGTGCGTCTCTCAGCGCCGCCTCTCATGAAGCCTGCCTTCATAGGAGGATTGCACAACTGAAGGAGCAGGAGTGCGAGACTGTTGTGGAAGATTTCATGTACCTCTTAGTCTTTTACAAGTTTTCCGAGATCAAAGTTCATTTGGTTCCTAAGCTCTCTAGATGCATTTACAATGGGAGACTGGAGATATGGCCTTCAAAGGATTGGGAGCTAGAGTCCCTTTACAGCATGGACGTTTTGGAGATGATAAGGGAACATGTAAGCACAGTCATTGGCTTGAGAGCAAAGTCTAGCGTCACAGACAATTGGGCAATGACAAAGATCACGCGTCAAACACTTGGTCAAGCATATGTGGCTTCCATCTTATATGGTTACTTTCTGAAATCTGCCTCATTGAGACACAGCCTGGATTGCATTCTAACTCTGGAGAGCCAAGACCTTCCTCTGAATCACAGAACCTCTCTTCAATTTCTGGAAATGTGCCCTAATGGAAACAAAAATCTTCTCTTTGGCCGCATTGGCAACATTCAAGCGAAGTTTCAAGGGTCAAGTAGGCAGGAGAAGACTCATGGAAAGTTGAGGTGTTACGTGATGGGGTTTGATCCTGATACACTTCAGAGATGCGCAAAACTGAGATCCGAGGTCGCTGTGAATTTAATTAAGAATCACTGTTGTGCACTCTTTGGCGATGATGAGAGCATGGGTTCGCCTGAGACCGATGAAGTTATCTTAACTTCTTATTCAAGCCTGAAGAGGCTGGTTCTAGAGGCTGTCGCCTTTGGTTCATTCCTCTGGGACACCGAAGAATGCATTGGGACAGTATATAAGCTTAAGGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF760

Protein Analysis

380

Amino Acids

42.68

Weight (kDa)

7.92

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF760 PF05542 112 - 239 3.6e-17 Protein of unknown function (DUF760)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07310 AT3G07310 AT5G48590
fragaria_vesca FvH4_2g18230 FvH4_2g18230 FvH4_2g18230
malus_domestica MD05G1166700.v1.1 MD10G1156700.v1.1
prunus_persica Prupe.8G195000_v2.0.a1
pyrus_communis pycom05g15550 pycom10g13490
rosa_chinensis RchiOBHm_Chr6g0283601
rosa_laevigata RLG00000012813
rosa_multiflora Rmu_co8416891.1_g000001
rosa_roxburghii Rroxscaffold_7G00185360
rosa_rugosa Rorug06G0156000
rosa_samantha Rh6AG267800 Rh6BG270300 Rh6CG270100 Rh6DG263800
rosa_wichuraiana Rw6G023200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 922
AccBSI CCGCTC 3 cut(s) 22, 69, 172
AccII CGCG 3 cut(s) 181, 229, 625
AclWI GGATC 2 cut(s) 893, 927
AcoI YGGCCR 2 cut(s) 270, 808
AcsI RAATTY 1 cut(s) 949
AcuI CTGAAG 5 cut(s) 88, 281, 377, 896, 1067
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 4 cut(s) 96, 132, 247, 279
AflII CTTAAG 1 cut(s) 1127
AflIII ACRYGT 2 cut(s) 553, 623
AgsI TTSAA 5 cut(s) 495, 764, 827, 839, 1041
AjnI CCWGG 1 cut(s) 702
AjuI GAANNNNNNNTTGG 2 cut(s) 1092, 1124
AleI CACNNNNGTG 1 cut(s) 969
AluBI AGCT 5 cut(s) 37, 259, 451, 508, 1126
AluI AGCT 5 cut(s) 37, 259, 451, 508, 1126
Alw21I GWGCWC 1 cut(s) 976
Alw26I GTCTC 6 cut(s) 17, 309, 368, 469, 688, 1004
Alw44I GTGCAC 1 cut(s) 972
AlwI GGATC 2 cut(s) 893, 927
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 4 cut(s) 107, 270, 488, 808
ApaLI GTGCAC 1 cut(s) 972
ApeKI GCWGC 1 cut(s) 156
ApoI RAATTY 1 cut(s) 949
ArsI GACNNNNNNTTYG 2 cut(s) 622, 654
AspLEI GCGC 2 cut(s) 314, 923
AspS9I GGNCC 3 cut(s) 4, 183, 199
AsuC2I CCSGG 2 cut(s) 133, 192
AvaII GGWCC 3 cut(s) 4, 183, 199
BaeGI GKGCMC 2 cut(s) 782, 976
BauI CACGAG 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 866
Bbv12I GWGCWC 1 cut(s) 976
BbvI GCAGC 1 cut(s) 168
BccI CCATC 3 cut(s) 231, 665, 883
BceAI ACGGC 2 cut(s) 59, 94
BciT130I CCWGG 1 cut(s) 704
BcnI CCSGG 2 cut(s) 133, 192
BcoDI GTCTC 6 cut(s) 17, 309, 368, 469, 688, 1004
BfaI CTAG 4 cut(s) 456, 509, 588, 1061
BfoI RGCGCY 1 cut(s) 315
BfrI CTTAAG 1 cut(s) 1127
BglI GCCNNNNNGGC 1 cut(s) 816
BisI GCNGC 5 cut(s) 157, 170, 230, 315, 811
BlsI GCNGC 5 cut(s) 158, 171, 231, 316, 812
Bme1390I CCNGG 3 cut(s) 133, 192, 704
Bme18I GGWCC 3 cut(s) 4, 183, 199
BmgT120I GGNCC 3 cut(s) 4, 183, 199
BmiI GGNNCC 3 cut(s) 130, 185, 444
BmrFI CCNGG 3 cut(s) 133, 192, 704
BmsI GCATC 2 cut(s) 449, 908
BoxI GACNNNNGTC 2 cut(s) 9, 119
BpiI GAAGAC 1 cut(s) 866
BpmI CTGGAG 2 cut(s) 500, 743
Bpu10I CCTNAGC 2 cut(s) 252, 447
BpuEI CTTGAG 2 cut(s) 17, 595
BpuMI CCSGG 2 cut(s) 133, 192
BsaAI YACGTR 1 cut(s) 886
BsaI GGTCTC 1 cut(s) 1004
BsaJI CCNNGG 5 cut(s) 132, 179, 227, 246, 936
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 497, 527
Bsc4I CCNNNNNNNGG 4 cut(s) 96, 132, 247, 279
Bse1I ACTGG 1 cut(s) 483
Bse3DI GCAATG 1 cut(s) 615
BseBI CCWGG 1 cut(s) 704
BseDI CCNNGG 5 cut(s) 132, 179, 227, 246, 936
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 4 cut(s) 96, 132, 247, 279
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 4 cut(s) 266, 322, 920, 999
BseNI ACTGG 1 cut(s) 483
BseSI GKGCMC 2 cut(s) 782, 976
BseXI GCAGC 1 cut(s) 168
BsgI GTGCAG 1 cut(s) 190
Bsh1236I CGCG 3 cut(s) 181, 229, 625
BshFI GGCC 4 cut(s) 109, 272, 490, 810
BsiHKAI GWGCWC 1 cut(s) 976
BsiSI CCGG 3 cut(s) 126, 132, 192
BslFI GGGAC 3 cut(s) 499, 1106, 1126
BslI CCNNNNNNNGG 4 cut(s) 96, 132, 247, 279
BsmAI GTCTC 6 cut(s) 17, 309, 368, 469, 688, 1004
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 3 cut(s) 499, 1106, 1126
BsmI GAATGC 2 cut(s) 711, 1109
BsnI GGCC 4 cut(s) 109, 272, 490, 810
Bso31I GGTCTC 1 cut(s) 1004
Bsp1286I GDGCHC 2 cut(s) 782, 976
Bsp143I GATC 5 cut(s) 277, 426, 618, 898, 932
BspANI GGCC 4 cut(s) 109, 272, 490, 810
BspCNI CTCAG 4 cut(s) 265, 321, 921, 1000
BspFNI CGCG 3 cut(s) 181, 229, 625
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 3 cut(s) 130, 185, 444
BspPI GGATC 2 cut(s) 893, 927
BspTI CTTAAG 1 cut(s) 1127
BspTNI GGTCTC 1 cut(s) 1004
BsrBI CCGCTC 3 cut(s) 22, 69, 172
BsrDI GCAATG 1 cut(s) 615
BsrI ACTGG 1 cut(s) 483
BssECI CCNNGG 5 cut(s) 132, 179, 227, 246, 936
BssMI GATC 5 cut(s) 277, 426, 618, 898, 932
BssSI CACGAG 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 704
Bst4CI ACNGT 6 cut(s) 118, 211, 379, 565, 968, 1117
Bst6I CTCTTC 2 cut(s) 765, 1043
BstAFI CTTAAG 1 cut(s) 1127
BstBAI YACGTR 1 cut(s) 886
BstC8I GCNNGC 2 cut(s) 139, 331
BstDEI CTNAG 6 cut(s) 252, 308, 403, 447, 929, 1008
BstDSI CCRYGG 3 cut(s) 179, 227, 246
BstF5I GGATG 1 cut(s) 223
BstFNI CGCG 3 cut(s) 181, 229, 625
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 2 cut(s) 314, 923
BstKTI GATC 5 cut(s) 280, 429, 621, 901, 935
BstMAI GTCTC 6 cut(s) 17, 309, 368, 469, 688, 1004
BstMBI GATC 5 cut(s) 277, 426, 618, 898, 932
BstMWI GCNNNNNNNGC 5 cut(s) 153, 178, 650, 708, 816
BstNI CCWGG 1 cut(s) 704
BstNSI RCATGY 1 cut(s) 557
BstPAI GACNNNNGTC 2 cut(s) 9, 119
BstSCI CCNGG 3 cut(s) 131, 190, 702
BstSLI GKGCMC 2 cut(s) 782, 976
BstUI CGCG 3 cut(s) 181, 229, 625
BstV1I GCAGC 1 cut(s) 168
BstV2I GAAGAC 1 cut(s) 866
BstX2I RGATCY 1 cut(s) 932
BstYI RGATCY 1 cut(s) 932
BsuRI GGCC 4 cut(s) 109, 272, 490, 810
BtgI CCRYGG 3 cut(s) 179, 227, 246
BtgZI GCGATG 1 cut(s) 999
BtsCI GGATG 1 cut(s) 223
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 2 cut(s) 142, 964
Cac8I GCNNGC 2 cut(s) 139, 331
CaiI CAGNNNCTG 1 cut(s) 259
CciI TCATGA 1 cut(s) 322
CfoI GCGC 2 cut(s) 314, 923
Cfr13I GGNCC 3 cut(s) 4, 183, 199
Cfr42I CCGCGG 2 cut(s) 182, 230
CseI GACGC 3 cut(s) 291, 580, 614
Csp6I GTAC 1 cut(s) 397
CviAII CATG 6 cut(s) 323, 394, 526, 554, 866, 997
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 6 cut(s) 252, 308, 403, 447, 929, 1008
DpnI GATC 5 cut(s) 279, 428, 620, 900, 934
DpnII GATC 5 cut(s) 277, 426, 618, 898, 932
EaeI YGGCCR 2 cut(s) 270, 808
Eam1104I CTCTTC 2 cut(s) 765, 1043
EarI CTCTTC 2 cut(s) 765, 1043
EciI GGCGGA 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 1004
Eco47I GGWCC 3 cut(s) 4, 183, 199
Eco57I CTGAAG 5 cut(s) 88, 281, 377, 896, 1067
EcoO109I RGGNCCY 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 702
EcoT22I ATGCAT 2 cut(s) 464, 1109
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 6 cut(s) 326, 397, 529, 557, 869, 1000
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 3 cut(s) 499, 1106, 1126
FatI CATG 6 cut(s) 322, 393, 525, 553, 865, 996
FauI CCCGC 5 cut(s) 27, 58, 69, 74, 186
FauNDI CATATG 1 cut(s) 646
Fnu4HI GCNGC 5 cut(s) 157, 170, 230, 315, 811
FokI GGATG 1 cut(s) 210
Fsp4HI GCNGC 5 cut(s) 157, 170, 230, 315, 811
FspBI CTAG 4 cut(s) 456, 509, 588, 1061
FspI TGCGCA 1 cut(s) 922
GlaI GCGC 2 cut(s) 313, 922
GluI GCNGC 5 cut(s) 157, 170, 230, 315, 811
GsuI CTGGAG 2 cut(s) 500, 743
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 4 cut(s) 109, 272, 490, 810
HapII CCGG 3 cut(s) 126, 132, 192
HgaI GACGC 3 cut(s) 291, 580, 614
HhaI GCGC 2 cut(s) 314, 923
Hin1II CATG 6 cut(s) 326, 397, 529, 557, 869, 1000
Hin6I GCGC 2 cut(s) 312, 921
HinP1I GCGC 2 cut(s) 312, 921
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 4 cut(s) 512, 745, 862, 961
HpaII CCGG 3 cut(s) 126, 132, 192
Hpy166II GTNNAC 2 cut(s) 114, 974
Hpy188I TCNGA 5 cut(s) 424, 678, 744, 915, 937
Hpy188III TCNNGA 8 cut(s) 254, 281, 323, 456, 722, 770, 902, 1061
Hpy8I GTNNAC 2 cut(s) 114, 974
Hpy99I CGWCG 2 cut(s) 122, 125
HpyAV CCTTC 5 cut(s) 64, 344, 352, 501, 746
HpyCH4III ACNGT 6 cut(s) 118, 211, 379, 565, 968, 1117
HpyCH4IV ACGT 2 cut(s) 531, 885
HpyCH4V TGCA 8 cut(s) 42, 207, 217, 350, 462, 711, 974, 1107
HpyF10VI GCNNNNNNNGC 5 cut(s) 153, 178, 650, 708, 816
HpyF3I CTNAG 6 cut(s) 252, 308, 403, 447, 929, 1008
HpySE526I ACGT 2 cut(s) 531, 885
Hsp92II CATG 6 cut(s) 326, 397, 529, 557, 869, 1000
HspAI GCGC 2 cut(s) 312, 921
KspI CCGCGG 2 cut(s) 182, 230
Kzo9I GATC 5 cut(s) 277, 426, 618, 898, 932
LmnI GCTCC 5 cut(s) 74, 128, 256, 361, 505
Lsp1109I GCAGC 1 cut(s) 168
LweI GCATC 2 cut(s) 449, 908
MaeI CTAG 4 cut(s) 456, 509, 588, 1061
MaeII ACGT 2 cut(s) 531, 885
MaeIII GTNAC 3 cut(s) 592, 668, 881
MalI GATC 5 cut(s) 279, 428, 620, 900, 934
MbiI CCGCTC 3 cut(s) 22, 69, 172
MboI GATC 5 cut(s) 277, 426, 618, 898, 932
MboII GAAGA 8 cut(s) 94, 274, 398, 752, 791, 871, 1060, 1112
MfeI CAATTG 1 cut(s) 601
MflI RGATCY 1 cut(s) 932
MhlI GDGCHC 2 cut(s) 782, 976
MluCI AATT 4 cut(s) 601, 764, 949, 954
MluI ACGCGT 1 cut(s) 623
MlyI GAGTC 2 cut(s) 521, 856
Mph1103I ATGCAT 2 cut(s) 464, 1109
MseI TTAA 4 cut(s) 953, 957, 1028, 1128
MslI CAYNNNNRTG 1 cut(s) 969
MspA1I CMGCKG 2 cut(s) 181, 229
MspCI CTTAAG 1 cut(s) 1127
MspI CCGG 3 cut(s) 126, 132, 192
MspR9I CCNGG 3 cut(s) 133, 192, 704
MunI CAATTG 1 cut(s) 601
Mva1269I GAATGC 2 cut(s) 711, 1109
MvaI CCWGG 1 cut(s) 704
MvnI CGCG 3 cut(s) 181, 229, 625
MwoI GCNNNNNNNGC 5 cut(s) 153, 178, 650, 708, 816
NciI CCSGG 2 cut(s) 133, 192
NdeI CATATG 1 cut(s) 646
NdeII GATC 5 cut(s) 277, 426, 618, 898, 932
NlaIII CATG 6 cut(s) 326, 397, 529, 557, 869, 1000
NlaIV GGNNCC 3 cut(s) 130, 185, 444
NmuCI GTSAC 1 cut(s) 592
NsbI TGCGCA 1 cut(s) 922
NsiI ATGCAT 2 cut(s) 464, 1109
NspI RCATGY 1 cut(s) 557
OliI CACNNNNGTG 1 cut(s) 969
PacI TTAATTAA 1 cut(s) 957
PagI TCATGA 1 cut(s) 322
PciI ACATGT 1 cut(s) 553
PctI GAATGC 2 cut(s) 711, 1109
PfeI GAWTC 2 cut(s) 745, 961
PkrI GCNGC 5 cut(s) 158, 171, 231, 316, 812
PleI GAGTC 2 cut(s) 520, 856
PpsI GAGTC 2 cut(s) 520, 856
Ppu21I YACGTR 1 cut(s) 886
PpuMI RGGWCCY 1 cut(s) 199
PscI ACATGT 1 cut(s) 553
PshAI GACNNNNGTC 2 cut(s) 9, 119
Psp5II RGGWCCY 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 702
PspGI CCWGG 1 cut(s) 702
PspN4I GGNNCC 3 cut(s) 130, 185, 444
PspPI GGNCC 3 cut(s) 4, 183, 199
PspPPI RGGWCCY 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 932
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
RseI CAYNNNNRTG 1 cut(s) 969
SacII CCGCGG 2 cut(s) 182, 230
SaqAI TTAA 4 cut(s) 953, 957, 1028, 1128
SatI GCNGC 5 cut(s) 157, 170, 230, 315, 811
Sau3AI GATC 5 cut(s) 277, 426, 618, 898, 932
Sau96I GGNCC 3 cut(s) 4, 183, 199
SchI GAGTC 2 cut(s) 521, 856
ScrFI CCNGG 3 cut(s) 133, 192, 704
SduI GDGCHC 2 cut(s) 782, 976
SfaNI GCATC 2 cut(s) 449, 908
Sfr303I CCGCGG 2 cut(s) 182, 230
SgrBI CCGCGG 2 cut(s) 182, 230
SinI GGWCC 3 cut(s) 4, 183, 199
SmiMI CAYNNNNRTG 1 cut(s) 969
SmlI CTYRAG 3 cut(s) 32, 574, 1127
SmoI CTYRAG 3 cut(s) 32, 574, 1127
Sse9I AATT 4 cut(s) 601, 764, 949, 954
SspMI CTAG 4 cut(s) 456, 509, 588, 1061
StyD4I CCNGG 3 cut(s) 131, 190, 702
TaaI ACNGT 6 cut(s) 118, 211, 379, 565, 968, 1117
TaiI ACGT 2 cut(s) 534, 888
TaqI TCGA 1 cut(s) 174
TaqII GACCGA 1 cut(s) 1028
TasI AATT 4 cut(s) 601, 764, 949, 954
TauI GCSGC 4 cut(s) 172, 232, 317, 813
TfiI GAWTC 2 cut(s) 745, 961
Tru1I TTAA 4 cut(s) 953, 957, 1028, 1128
Tru9I TTAA 4 cut(s) 953, 957, 1028, 1128
TscAI CASTG 2 cut(s) 149, 971
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 1 cut(s) 156
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 6 cut(s) 326, 339, 382, 425, 1032, 1071
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 2 cut(s) 149, 971
Vha464I CTTAAG 1 cut(s) 1127
VneI GTGCAC 1 cut(s) 972
VpaK11BI GGWCC 3 cut(s) 4, 183, 199
XapI RAATTY 1 cut(s) 949
XbaI TCTAGA 2 cut(s) 455, 1060
XceI RCATGY 1 cut(s) 557
XspI CTAG 4 cut(s) 456, 509, 588, 1061
Zsp2I ATGCAT 2 cut(s) 464, 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.