pycom05g15550

Protein of unknown function (DUF760)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010353
Physical Location & Seq
Forward (+)
19085768 .. 19087825
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g15550.2

Sequence Viewer

Length: 951 bp
ATGGACCATTGTCTCTCCCCGCTCTCAACCGTCAAGCTCTGCATAACCCCCGCCTTCGCCCCGCCCCGCTCCGCCGTCCAATCGCCGACCGCAAATCGGTTCTTCAGGCCGTTGACCGTCGTCGCCGGAGCCGGGGCGAGCCACTGCGAGTTCAGCAGCCTCAACTCGCCGCTCGACCCGCGGACCCGACCCGGAAAGGACCTCTGCACCGTCTTGCAGAACCATCCGCGGCTGTTCCACCTTTCCGTGGCTCAGGAGCTGAAGAAGTTGGCCGATGATCGGGAACTCGCTCTCTCTCGTGCGTCTCTCAGCGCCGCCTCTCATGAAGCCTGCCTTCATAGGAGGATTGCACAACTGAAGGAGCAGGAGTGCGAGACTGTTGTGGAAGATTTCATGTACCTCTTAGTCTTTTACAAGTTTTCCGAGATCAAAGTTCATTTGGTTCCTAAGCTCTCTAGATGCATTTACAATAGGAGACTGGAGATATGGCCTTCAAAGGATTGGGAGCTAGAGTCCCTTTACAGCATGGACGTTTTGGAGATGATAAGGGAACATGTAAGCACAGTCATTGGCTTGAGAGCAAAGTCTAGCGTCACAGACAATTGGGCAATGACAAAGATCACGCGTCAAACACTTGGTCAAGCATATGTGGCTTCCATCTTATATGGTTACTTTCTGAAATCTGCCTCATCGAGACATAGCCTGGATCACATTCTAACTCTGGAGAGCCAAGACCTTCCTCTGAATCACAGAACCTCTCTTCAATTTCTGGAAATGTGTCCTAATGGAAACAAAAATCTTCTCTTTGGCCGCATTGGCAACATTCAAACAAAGTTTCAAGGGTCAAGTAGGCAGGAGAAGACTCATGGAGAGTTGAGGTGTTATGTGATGGGGTTTGATCGTGATACACTTCAGAGATGCGCAAAACTGATCGATCCAAGGTGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF760

Protein Analysis

317

Amino Acids

35.99

Weight (kDa)

8.98

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07310 AT3G07310 AT5G48590
fragaria_vesca FvH4_2g18230 FvH4_2g18230 FvH4_2g18230
malus_domestica MD05G1166700.v1.1 MD10G1156700.v1.1
prunus_persica Prupe.8G195000_v2.0.a1
pyrus_communis pycom05g15550 pycom10g13490
rosa_chinensis RchiOBHm_Chr6g0283601
rosa_laevigata RLG00000012813
rosa_multiflora Rmu_co8416891.1_g000001
rosa_roxburghii Rroxscaffold_7G00185360
rosa_rugosa Rorug06G0156000
rosa_samantha Rh6AG267800 Rh6BG270300 Rh6CG270100 Rh6DG263800
rosa_wichuraiana Rw6G023200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 922
AccBSI CCGCTC 3 cut(s) 22, 69, 172
AccII CGCG 3 cut(s) 181, 229, 625
AclWI GGATC 2 cut(s) 714, 929
AcoI YGGCCR 2 cut(s) 270, 808
AcuI CTGAAG 4 cut(s) 88, 281, 377, 896
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 4 cut(s) 96, 132, 247, 279
AflIII ACRYGT 2 cut(s) 553, 623
AgsI TTSAA 4 cut(s) 495, 764, 827, 839
AjnI CCWGG 1 cut(s) 702
AluBI AGCT 4 cut(s) 37, 259, 451, 508
AluI AGCT 4 cut(s) 37, 259, 451, 508
Alw26I GTCTC 5 cut(s) 17, 309, 368, 469, 688
AlwI GGATC 2 cut(s) 714, 929
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 4 cut(s) 107, 270, 488, 808
ApeKI GCWGC 1 cut(s) 156
ArsI GACNNNNNNTTYG 2 cut(s) 622, 654
AspLEI GCGC 2 cut(s) 314, 923
AspS9I GGNCC 3 cut(s) 4, 183, 199
AsuC2I CCSGG 2 cut(s) 133, 192
AvaII GGWCC 3 cut(s) 4, 183, 199
BauI CACGAG 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 866
BbvI GCAGC 1 cut(s) 168
BccI CCATC 3 cut(s) 231, 665, 883
BceAI ACGGC 2 cut(s) 59, 94
BciT130I CCWGG 1 cut(s) 704
BcnI CCSGG 2 cut(s) 133, 192
BcoDI GTCTC 5 cut(s) 17, 309, 368, 469, 688
BfaI CTAG 3 cut(s) 456, 509, 588
BfoI RGCGCY 1 cut(s) 315
BglI GCCNNNNNGGC 1 cut(s) 816
BisI GCNGC 5 cut(s) 157, 170, 230, 315, 811
BlsI GCNGC 5 cut(s) 158, 171, 231, 316, 812
Bme1390I CCNGG 3 cut(s) 133, 192, 704
Bme18I GGWCC 3 cut(s) 4, 183, 199
BmgT120I GGNCC 3 cut(s) 4, 183, 199
BmiI GGNNCC 3 cut(s) 130, 185, 444
BmrFI CCNGG 3 cut(s) 133, 192, 704
BmsI GCATC 2 cut(s) 449, 908
BoxI GACNNNNGTC 2 cut(s) 9, 119
BpiI GAAGAC 1 cut(s) 866
BpmI CTGGAG 2 cut(s) 500, 743
Bpu10I CCTNAGC 2 cut(s) 252, 447
BpuEI CTTGAG 1 cut(s) 595
BpuMI CCSGG 2 cut(s) 133, 192
Bsa29I ATCGAT 1 cut(s) 933
BsaJI CCNNGG 5 cut(s) 132, 179, 227, 246, 938
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 497, 527
Bsc4I CCNNNNNNNGG 4 cut(s) 96, 132, 247, 279
Bse1I ACTGG 1 cut(s) 483
Bse3DI GCAATG 1 cut(s) 615
BseBI CCWGG 1 cut(s) 704
BseCI ATCGAT 1 cut(s) 933
BseDI CCNNGG 5 cut(s) 132, 179, 227, 246, 938
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 4 cut(s) 96, 132, 247, 279
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 2 cut(s) 266, 322
BseNI ACTGG 1 cut(s) 483
BseXI GCAGC 1 cut(s) 168
BsgI GTGCAG 1 cut(s) 190
Bsh1236I CGCG 3 cut(s) 181, 229, 625
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 4 cut(s) 109, 272, 490, 810
BshVI ATCGAT 1 cut(s) 933
BsiEI CGRYCG 1 cut(s) 90
BsiSI CCGG 3 cut(s) 126, 132, 192
BslFI GGGAC 1 cut(s) 499
BslI CCNNNNNNNGG 4 cut(s) 96, 132, 247, 279
BsmAI GTCTC 5 cut(s) 17, 309, 368, 469, 688
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 1 cut(s) 499
BsnI GGCC 4 cut(s) 109, 272, 490, 810
Bsp143I GATC 7 cut(s) 277, 426, 618, 706, 898, 930, 934
BspANI GGCC 4 cut(s) 109, 272, 490, 810
BspCNI CTCAG 2 cut(s) 265, 321
BspDI ATCGAT 1 cut(s) 933
BspFNI CGCG 3 cut(s) 181, 229, 625
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 3 cut(s) 130, 185, 444
BspPI GGATC 2 cut(s) 714, 929
BsrBI CCGCTC 3 cut(s) 22, 69, 172
BsrDI GCAATG 1 cut(s) 615
BsrI ACTGG 1 cut(s) 483
BssECI CCNNGG 5 cut(s) 132, 179, 227, 246, 938
BssMI GATC 7 cut(s) 277, 426, 618, 706, 898, 930, 934
BssSI CACGAG 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 938
Bst2BI CACGAG 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 704
Bst4CI ACNGT 5 cut(s) 31, 118, 211, 379, 565
Bst6I CTCTTC 1 cut(s) 765
BstC8I GCNNGC 2 cut(s) 139, 331
BstDEI CTNAG 4 cut(s) 252, 308, 403, 447
BstDSI CCRYGG 3 cut(s) 179, 227, 246
BstF5I GGATG 1 cut(s) 223
BstFNI CGCG 3 cut(s) 181, 229, 625
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 2 cut(s) 314, 923
BstKTI GATC 7 cut(s) 280, 429, 621, 709, 901, 933, 937
BstMAI GTCTC 5 cut(s) 17, 309, 368, 469, 688
BstMBI GATC 7 cut(s) 277, 426, 618, 706, 898, 930, 934
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 4 cut(s) 153, 178, 650, 816
BstNI CCWGG 1 cut(s) 704
BstNSI RCATGY 1 cut(s) 557
BstPAI GACNNNNGTC 2 cut(s) 9, 119
BstSCI CCNGG 3 cut(s) 131, 190, 702
BstUI CGCG 3 cut(s) 181, 229, 625
BstV1I GCAGC 1 cut(s) 168
BstV2I GAAGAC 1 cut(s) 866
Bsu15I ATCGAT 1 cut(s) 933
BsuRI GGCC 4 cut(s) 109, 272, 490, 810
BsuTUI ATCGAT 1 cut(s) 933
BtgI CCRYGG 3 cut(s) 179, 227, 246
BtsCI GGATG 1 cut(s) 223
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 2 cut(s) 139, 331
CaiI CAGNNNCTG 1 cut(s) 259
CciI TCATGA 1 cut(s) 322
CfoI GCGC 2 cut(s) 314, 923
Cfr13I GGNCC 3 cut(s) 4, 183, 199
Cfr42I CCGCGG 2 cut(s) 182, 230
ClaI ATCGAT 1 cut(s) 933
CseI GACGC 3 cut(s) 291, 580, 614
Csp6I GTAC 1 cut(s) 397
CviAII CATG 5 cut(s) 323, 394, 526, 554, 866
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 4 cut(s) 252, 308, 403, 447
DpnI GATC 7 cut(s) 279, 428, 620, 708, 900, 932, 936
DpnII GATC 7 cut(s) 277, 426, 618, 706, 898, 930, 934
EaeI YGGCCR 2 cut(s) 270, 808
Eam1104I CTCTTC 1 cut(s) 765
EarI CTCTTC 1 cut(s) 765
EciI GGCGGA 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 938
Eco47I GGWCC 3 cut(s) 4, 183, 199
Eco57I CTGAAG 4 cut(s) 88, 281, 377, 896
EcoO109I RGGNCCY 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 702
EcoT14I CCWWGG 1 cut(s) 938
EcoT22I ATGCAT 1 cut(s) 464
ErhI CCWWGG 1 cut(s) 938
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 5 cut(s) 326, 397, 529, 557, 869
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 1 cut(s) 499
FatI CATG 5 cut(s) 322, 393, 525, 553, 865
FauI CCCGC 5 cut(s) 27, 58, 69, 74, 186
FauNDI CATATG 1 cut(s) 646
Fnu4HI GCNGC 5 cut(s) 157, 170, 230, 315, 811
FokI GGATG 1 cut(s) 210
Fsp4HI GCNGC 5 cut(s) 157, 170, 230, 315, 811
FspBI CTAG 3 cut(s) 456, 509, 588
FspI TGCGCA 1 cut(s) 922
GlaI GCGC 2 cut(s) 313, 922
GluI GCNGC 5 cut(s) 157, 170, 230, 315, 811
GsuI CTGGAG 2 cut(s) 500, 743
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 4 cut(s) 109, 272, 490, 810
HapII CCGG 3 cut(s) 126, 132, 192
HgaI GACGC 3 cut(s) 291, 580, 614
HhaI GCGC 2 cut(s) 314, 923
Hin1II CATG 5 cut(s) 326, 397, 529, 557, 869
Hin6I GCGC 2 cut(s) 312, 921
HinP1I GCGC 2 cut(s) 312, 921
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 3 cut(s) 512, 745, 862
HpaII CCGG 3 cut(s) 126, 132, 192
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 4 cut(s) 424, 678, 744, 915
Hpy188III TCNNGA 8 cut(s) 254, 281, 323, 456, 693, 722, 770, 902
Hpy8I GTNNAC 1 cut(s) 114
Hpy99I CGWCG 2 cut(s) 122, 125
HpyAV CCTTC 5 cut(s) 64, 344, 352, 501, 746
HpyCH4III ACNGT 5 cut(s) 31, 118, 211, 379, 565
HpyCH4IV ACGT 1 cut(s) 531
HpyCH4V TGCA 5 cut(s) 42, 207, 217, 350, 462
HpyF10VI GCNNNNNNNGC 4 cut(s) 153, 178, 650, 816
HpyF3I CTNAG 4 cut(s) 252, 308, 403, 447
HpySE526I ACGT 1 cut(s) 531
Hsp92II CATG 5 cut(s) 326, 397, 529, 557, 869
HspAI GCGC 2 cut(s) 312, 921
KspI CCGCGG 2 cut(s) 182, 230
Kzo9I GATC 7 cut(s) 277, 426, 618, 706, 898, 930, 934
LmnI GCTCC 5 cut(s) 74, 128, 256, 361, 505
Lsp1109I GCAGC 1 cut(s) 168
LweI GCATC 2 cut(s) 449, 908
MaeI CTAG 3 cut(s) 456, 509, 588
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 2 cut(s) 592, 668
MalI GATC 7 cut(s) 279, 428, 620, 708, 900, 932, 936
MbiI CCGCTC 3 cut(s) 22, 69, 172
MboI GATC 7 cut(s) 277, 426, 618, 706, 898, 930, 934
MboII GAAGA 6 cut(s) 94, 274, 398, 752, 791, 871
MfeI CAATTG 1 cut(s) 601
MluCI AATT 2 cut(s) 601, 764
MluI ACGCGT 1 cut(s) 623
MlyI GAGTC 2 cut(s) 521, 856
MnlI CCTC 9 cut(s) 170, 212, 328, 336, 410, 697, 750, 766, 870
Mph1103I ATGCAT 1 cut(s) 464
MspA1I CMGCKG 2 cut(s) 181, 229
MspI CCGG 3 cut(s) 126, 132, 192
MspR9I CCNGG 3 cut(s) 133, 192, 704
MunI CAATTG 1 cut(s) 601
MvaI CCWGG 1 cut(s) 704
MvnI CGCG 3 cut(s) 181, 229, 625
MwoI GCNNNNNNNGC 4 cut(s) 153, 178, 650, 816
NciI CCSGG 2 cut(s) 133, 192
NdeI CATATG 1 cut(s) 646
NdeII GATC 7 cut(s) 277, 426, 618, 706, 898, 930, 934
NlaIII CATG 5 cut(s) 326, 397, 529, 557, 869
NlaIV GGNNCC 3 cut(s) 130, 185, 444
NmuCI GTSAC 1 cut(s) 592
NsbI TGCGCA 1 cut(s) 922
NsiI ATGCAT 1 cut(s) 464
NspI RCATGY 1 cut(s) 557
PagI TCATGA 1 cut(s) 322
PciI ACATGT 1 cut(s) 553
PfeI GAWTC 1 cut(s) 745
PkrI GCNGC 5 cut(s) 158, 171, 231, 316, 812
PleI GAGTC 2 cut(s) 520, 856
PpsI GAGTC 2 cut(s) 520, 856
PpuMI RGGWCCY 1 cut(s) 199
PscI ACATGT 1 cut(s) 553
PshAI GACNNNNGTC 2 cut(s) 9, 119
Psp5II RGGWCCY 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 702
PspGI CCWGG 1 cut(s) 702
PspN4I GGNNCC 3 cut(s) 130, 185, 444
PspPI GGNCC 3 cut(s) 4, 183, 199
PspPPI RGGWCCY 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 259
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
SacII CCGCGG 2 cut(s) 182, 230
SatI GCNGC 5 cut(s) 157, 170, 230, 315, 811
Sau3AI GATC 7 cut(s) 277, 426, 618, 706, 898, 930, 934
Sau96I GGNCC 3 cut(s) 4, 183, 199
SchI GAGTC 2 cut(s) 521, 856
ScrFI CCNGG 3 cut(s) 133, 192, 704
SfaNI GCATC 2 cut(s) 449, 908
Sfr303I CCGCGG 2 cut(s) 182, 230
SgrBI CCGCGG 2 cut(s) 182, 230
SinI GGWCC 3 cut(s) 4, 183, 199
SmlI CTYRAG 1 cut(s) 574
SmoI CTYRAG 1 cut(s) 574
Sse9I AATT 2 cut(s) 601, 764
SspMI CTAG 3 cut(s) 456, 509, 588
StyD4I CCNGG 3 cut(s) 131, 190, 702
StyI CCWWGG 1 cut(s) 938
TaaI ACNGT 5 cut(s) 31, 118, 211, 379, 565
TaiI ACGT 1 cut(s) 534
TaqI TCGA 3 cut(s) 174, 692, 933
TasI AATT 2 cut(s) 601, 764
TauI GCSGC 4 cut(s) 172, 232, 317, 813
TfiI GAWTC 1 cut(s) 745
TscAI CASTG 1 cut(s) 149
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 1 cut(s) 156
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 4 cut(s) 326, 339, 382, 425
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 1 cut(s) 149
VpaK11BI GGWCC 3 cut(s) 4, 183, 199
XbaI TCTAGA 1 cut(s) 455
XceI RCATGY 1 cut(s) 557
XspI CTAG 3 cut(s) 456, 509, 588
Zsp2I ATGCAT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.