RLG00000012813

Protein of unknown function (DUF760)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010353
Physical Location & Seq
Reverse (-)
22592660 .. 22599206
6547 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012813

Sequence Viewer

Length: 1950 bp
ATGGAACATTGTCTCTCCCACCACAAGCTCCTCGCTCTTCCGTCCCCGCTCTCCGCCTTGAAGCCGCGATTAGCGCTCAATTTCGCGCCCAAATCCCAGGCGTTGACCAGGCGGTTGACTGTGGTAGCCGGCGCCGGGGCGTCGAGCCACTGCGAGTTCAGCAGCCTCAACTCGCCGCTCGACCCGAGGACCCGATCCGGCAAGGACCTCAGCTCTGTTTTGCAAAACCACCCGCAGCTGTTTCACTTGGCCGTCGCTCAGGAGCTCAAGCAGTTGGCCGATCAGCGACAGGACGCGCGATGTCGTATGCATCTCAGCGCTTCGTCTCGTGAGGCCTGCCTTCATAGGAGGATTGCTCAACTGAAGGAGCAGCAGTGCCAGATTGCTGTTGAAGATGTCATGTACCTGTTGATCTTTTACAAGTTTTCTGAGGTCAAAGTTCCTTTGGTTCCTAAACTTTCTAGATGCATCTACAATGATAGACTGGAGATATTGCCTGCAAAGGATTGGGAGCTAGAGTCCATTTACAGCTTGGAGGTTTTGGAGATGATAAGGGAACATGTCACCACTGTCATTGGCTTGAGAGCAAATTCTAGTGTCACAGAAAATTGGGCAATGACAAAGATAACCCGCCAGACGCTTGGCCGAGTCTATGTGGCCTCCATCTTATATGGTTACTTCTTGAAATCTGTCTCATTGAGGCATCGCCTGGAACGAAGTCTATTTCTTGAAACCCAAGACCTTCATCTGAGTTGTAGAACCTCCCTTCAGGAAATGTCTCCTCATGGAATAAAAAGTCTTCTCTTTGGCCACGTCGGCAAATTTCAATCGGAGTGTGTAGGGTCAAGTAGGCAGGAAAAGACACATGGAAAGTTGAAATGTTATGTGATGGGGTTTGATCCCGAGACACTACAGAGATGTGCAAAATTGAGATCTGAGGAGGCTGTGAATTTAATTAAAAATCACTGTTGTGCACTTTTTGGGGATGATGCGGAAATGGGTTCTCTTGAGTCGGATGAAGTTATCTCTACTTCATTTTCAAGCCTGAAGAGGCTAGTTTTGGAGGCTGTTGCTTTCGGTTCTTTCCTTTGGGACACAGAAGAATGCATTGATAGTAAGGGCATCCTGCAGCCTCCTTTCCCACACAAAGCCCTGATATACCAGCATGTAGAGATTTCAATCCCCAGTCCTAAGAGAGATGAGGTTTTAGTGAAATTAGAAGCAGCTAGCCTAAATCCAGCTGATTGGAAAATTCAGAAAGGCATGCTGCGTCCTCTTGTTCCACGCAAATTCCCTTTTATACCTGGATTTGATGTGGCTGGAGAGATTGTAGAGGTTGGAGAAAGAGTTAAAAAGTTCAAAGCCAGTGATAAAGTTGTGGCAGTGCTCCACCTTTTGGATGGAGGTGGACTGGCTGAGTTTGCTACAGCTAGTGAGAAATTGACAGTTGCTAGGCCCCAAGAAGTTTCAGCAGCTGAAGGTGCAGGCTTACCTACTTGTGGTCTCACAGCTCACCAGGCTCTAACACAAGCTGGAGAGATCGAGCTTGATGGAACTGGCCAGCTGAAGAGCATACTCATTACTGGTGCTTCTGGTGGTGTTGGCCATTATGCAGTCCAACTGGCAGAGCTGGCGAACACTCATGTGACAGTGACTTGTGGAGCTCGTAACATCGAATTTGGCAAGAGATTGGGGGCTGATGAAGTTCTCGACTACGGTACCCCAGATGGGGCAGCTTTAAGGAGTCCATCTGTTACCTTCTCCAAGAAGAAGCTGGTGCCTTTGATCATAAATGCCAAGGCTGAAAACGTTGATTATCTTTTGAAGTTAGTGAAGGAAGGAAAGCTGAAGACAGTGATCGACTCAAAGCATTCTCTAAGCAAGGCTGAAGATGCCTGGGCTAAAAGTATGGATGGTCATGCTACTGGGAAGATCATTTTACAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF760

Protein Analysis

650

Amino Acids

71.42

Weight (kDa)

8.47

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF760 PF05542 114 - 241 2.5e-18 Protein of unknown function (DUF760)
ADH_N PF08240 399 - 484 6.6e-15 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N_2 PF13602 590 - 647 4.7e-10 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07310 AT3G07310 AT5G48590
fragaria_vesca FvH4_2g18230 FvH4_2g18230 FvH4_2g18230
malus_domestica MD05G1166700.v1.1 MD10G1156700.v1.1
prunus_persica Prupe.8G195000_v2.0.a1
pyrus_communis pycom05g15550 pycom10g13490
rosa_chinensis RchiOBHm_Chr6g0283601
rosa_laevigata RLG00000012813
rosa_multiflora Rmu_co8416891.1_g000001
rosa_roxburghii Rroxscaffold_7G00185360
rosa_rugosa Rorug06G0156000
rosa_samantha Rh6AG267800 Rh6BG270300 Rh6CG270100 Rh6DG263800
rosa_wichuraiana Rw6G023200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1718
AccB1I GGYRCC 3 cut(s) 131, 1718, 1777
AccB7I CCANNNNNTGG 1 cut(s) 1396
AccBSI CCGCTC 2 cut(s) 49, 178
AccII CGCG 4 cut(s) 67, 86, 296, 298
AciI CCGC 8 cut(s) 47, 54, 65, 112, 176, 233, 631, 992
AclI AACGTT 1 cut(s) 1809
AclWI GGATC 2 cut(s) 189, 893
AcoI YGGCCR 6 cut(s) 249, 276, 643, 808, 1558, 1603
AcsI RAATTY 6 cut(s) 589, 821, 949, 1251, 1289, 1676
AcuI CTGAAG 7 cut(s) 383, 752, 1067, 1497, 1586, 1868, 1908
AcyI GRCGYC 2 cut(s) 132, 140
AfaI GTAC 2 cut(s) 404, 1720
AfeI AGCGCT 2 cut(s) 75, 319
AfiI CCNNNNNNNGG 5 cut(s) 135, 1396, 1499, 1728, 1729
AflIII ACRYGT 1 cut(s) 559
AjiI CACGTC 1 cut(s) 814
AjnI CCWGG 6 cut(s) 96, 107, 708, 1303, 1515, 1895
AleI CACNNNNGTG 2 cut(s) 969, 1643
Alw21I GWGCWC 4 cut(s) 267, 976, 1389, 1666
Alw26I GTCTC 6 cut(s) 17, 330, 697, 783, 899, 1508
Alw44I GTGCAC 1 cut(s) 972
AlwI GGATC 2 cut(s) 189, 893
AlwNI CAGNNNCTG 1 cut(s) 1475
Ama87I CYCGRG 2 cut(s) 184, 902
Aor51HI AGCGCT 2 cut(s) 75, 319
AoxI GGCC 9 cut(s) 249, 276, 333, 643, 657, 808, 1454, 1558, 1603
ApaLI GTGCAC 1 cut(s) 972
ApeKI GCWGC 8 cut(s) 162, 235, 370, 1129, 1223, 1267, 1472, 1733
ApoI RAATTY 6 cut(s) 589, 821, 949, 1251, 1289, 1676
Asp718I GGTACC 1 cut(s) 1718
AspLEI GCGC 5 cut(s) 76, 88, 134, 298, 320
AspS9I GGNCC 3 cut(s) 189, 205, 1455
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 556, 1505
AsuNHI GCTAGC 1 cut(s) 1226
AvaI CYCGRG 2 cut(s) 184, 902
AvaII GGWCC 2 cut(s) 189, 205
BaeGI GKGCMC 1 cut(s) 976
BaeI ACNNNNGTAYC 2 cut(s) 1702, 1735
BalI TGGCCA 3 cut(s) 810, 1560, 1605
BanI GGYRCC 3 cut(s) 131, 1718, 1777
BanII GRGCYC 2 cut(s) 267, 1666
BauI CACGAG 1 cut(s) 327
BbsI GAAGAC 2 cut(s) 791, 1856
Bbv12I GWGCWC 4 cut(s) 267, 976, 1389, 1666
BbvCI CCTCAGC 1 cut(s) 209
BbvI GCAGC 8 cut(s) 174, 247, 382, 1141, 1235, 1254, 1484, 1745
BccI CCATC 7 cut(s) 671, 883, 1394, 1544, 1721, 1756, 1907
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 6 cut(s) 98, 109, 710, 1305, 1517, 1897
BclI TGATCA 1 cut(s) 1785
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 6 cut(s) 17, 330, 697, 783, 899, 1508
BfaI CTAG 7 cut(s) 462, 515, 594, 1055, 1227, 1431, 1452
BfmI CTRYAG 3 cut(s) 911, 1127, 1425
BfoI RGCGCY 3 cut(s) 77, 135, 321
BglI GCCNNNNNGGC 1 cut(s) 816
BglII AGATCT 1 cut(s) 932
Bme1390I CCNGG 7 cut(s) 98, 109, 136, 710, 1305, 1517, 1897
Bme18I GGWCC 2 cut(s) 189, 205
BmeT110I CYCGRG 2 cut(s) 184, 902
BmgBI CACGTC 1 cut(s) 814
BmgT120I GGNCC 3 cut(s) 189, 205, 1455
BmiI GGNNCC 6 cut(s) 133, 191, 450, 1457, 1720, 1779
BmrFI CCNGG 7 cut(s) 98, 109, 136, 710, 1305, 1517, 1897
BmrI ACTGGG 2 cut(s) 1179, 1935
BmsI GCATC 7 cut(s) 319, 455, 477, 712, 979, 1131, 1882
BmtI GCTAGC 1 cut(s) 1230
BmuI ACTGGG 2 cut(s) 1179, 1935
BpiI GAAGAC 2 cut(s) 791, 1856
BplI GAGNNNNNCTC 2 cut(s) 340, 372
BpmI CTGGAG 3 cut(s) 506, 1341, 1554
Bpu10I CCTNAGC 2 cut(s) 209, 258
BpuEI CTTGAG 3 cut(s) 251, 601, 1028
BpuMI CCSGG 1 cut(s) 136
BsaBI GATNNNNATC 1 cut(s) 1178
BsaHI GRCGYC 2 cut(s) 132, 140
BsaI GGTCTC 1 cut(s) 1508
BsaJI CCNNGG 5 cut(s) 96, 135, 185, 1797, 1896
BsaXI ACNNNNNCTCC 8 cut(s) 359, 389, 503, 533, 1314, 1332, 1344, 1362
Bsc4I CCNNNNNNNGG 5 cut(s) 135, 1396, 1499, 1728, 1729
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 8 cut(s) 489, 1185, 1365, 1416, 1561, 1588, 1626, 1930
Bse3DI GCAATG 1 cut(s) 621
Bse8I GATNNNNATC 1 cut(s) 1178
BseBI CCWGG 6 cut(s) 98, 109, 710, 1305, 1517, 1897
BseDI CCNNGG 5 cut(s) 96, 135, 185, 1797, 1896
BseGI GGATG 5 cut(s) 991, 1021, 1122, 1405, 1918
BseJI GATNNNNATC 1 cut(s) 1178
BseLI CCNNNNNNNGG 5 cut(s) 135, 1396, 1499, 1728, 1729
BseMI GCAATG 1 cut(s) 621
BseMII CTCAG 7 cut(s) 223, 272, 328, 420, 740, 927, 1407
BseNI ACTGG 8 cut(s) 489, 1185, 1365, 1416, 1561, 1588, 1626, 1930
BseRI GAGGAG 3 cut(s) 20, 771, 953
BseSI GKGCMC 1 cut(s) 976
BseXI GCAGC 8 cut(s) 174, 247, 382, 1141, 1235, 1254, 1484, 1745
BsgI GTGCAG 1 cut(s) 1503
Bsh1236I CGCG 4 cut(s) 67, 86, 296, 298
BshFI GGCC 9 cut(s) 251, 278, 335, 645, 659, 810, 1456, 1560, 1605
BshNI GGYRCC 3 cut(s) 131, 1718, 1777
BsiHKAI GWGCWC 4 cut(s) 267, 976, 1389, 1666
BsiHKCI CYCGRG 2 cut(s) 184, 902
BsiSI CCGG 3 cut(s) 129, 135, 198
BslFI GGGAC 2 cut(s) 28, 1106
BslI CCNNNNNNNGG 5 cut(s) 135, 1396, 1499, 1728, 1729
BsmAI GTCTC 6 cut(s) 17, 330, 697, 783, 899, 1508
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 2 cut(s) 28, 1106
BsmI GAATGC 2 cut(s) 1109, 1870
BsnI GGCC 9 cut(s) 251, 278, 335, 645, 659, 810, 1456, 1560, 1605
Bso31I GGTCTC 1 cut(s) 1508
BsoBI CYCGRG 2 cut(s) 184, 902
Bsp1286I GDGCHC 4 cut(s) 267, 976, 1389, 1666
Bsp143I GATC 9 cut(s) 194, 280, 411, 898, 932, 1539, 1785, 1857, 1932
BspACI CCGC 8 cut(s) 47, 54, 65, 112, 176, 233, 631, 992
BspANI GGCC 9 cut(s) 251, 278, 335, 645, 659, 810, 1456, 1560, 1605
BspCNI CTCAG 7 cut(s) 222, 271, 327, 421, 741, 928, 1408
BspFNI CGCG 4 cut(s) 67, 86, 296, 298
BspLI GGNNCC 6 cut(s) 133, 191, 450, 1457, 1720, 1779
BspMAI CTGCAG 1 cut(s) 1131
BspOI GCTAGC 1 cut(s) 1230
BspPI GGATC 2 cut(s) 189, 893
BspQI GCTCTTC 2 cut(s) 42, 1562
BspT107I GGYRCC 3 cut(s) 131, 1718, 1777
BspTNI GGTCTC 1 cut(s) 1508
BsrBI CCGCTC 2 cut(s) 49, 178
BsrDI GCAATG 1 cut(s) 621
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 8 cut(s) 489, 1185, 1365, 1416, 1561, 1588, 1626, 1930
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 5 cut(s) 96, 135, 185, 1797, 1896
BssMI GATC 9 cut(s) 194, 280, 411, 898, 932, 1539, 1785, 1857, 1932
BssNI GRCGYC 2 cut(s) 132, 140
BssSI CACGAG 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 1797
Bst2BI CACGAG 1 cut(s) 327
Bst2UI CCWGG 6 cut(s) 98, 109, 710, 1305, 1517, 1897
Bst4CI ACNGT 7 cut(s) 121, 571, 968, 1447, 1651, 1718, 1855
Bst6I CTCTTC 3 cut(s) 42, 1043, 1562
BstACI GRCGYC 2 cut(s) 132, 140
BstC8I GCNNGC 8 cut(s) 130, 337, 498, 1228, 1265, 1486, 1562, 1632
BstDEI CTNAG 9 cut(s) 209, 258, 314, 429, 749, 936, 1191, 1416, 1877
BstF5I GGATG 5 cut(s) 991, 1021, 1122, 1405, 1918
BstFNI CGCG 4 cut(s) 67, 86, 296, 298
BstH2I RGCGCY 3 cut(s) 77, 135, 321
BstHHI GCGC 5 cut(s) 76, 88, 134, 298, 320
BstKTI GATC 9 cut(s) 197, 283, 414, 901, 935, 1542, 1788, 1860, 1935
BstMAI GTCTC 6 cut(s) 17, 330, 697, 783, 899, 1508
BstMBI GATC 9 cut(s) 194, 280, 411, 898, 932, 1539, 1785, 1857, 1932
BstMWI GCNNNNNNNGC 8 cut(s) 73, 159, 816, 1421, 1481, 1517, 1631, 1892
BstNI CCWGG 6 cut(s) 98, 109, 710, 1305, 1517, 1897
BstNSI RCATGY 3 cut(s) 563, 1169, 1267
BstSCI CCNGG 7 cut(s) 96, 107, 134, 708, 1303, 1515, 1895
BstSFI CTRYAG 3 cut(s) 911, 1127, 1425
BstSLI GKGCMC 1 cut(s) 976
BstUI CGCG 4 cut(s) 67, 86, 296, 298
BstV1I GCAGC 8 cut(s) 174, 247, 382, 1141, 1235, 1254, 1484, 1745
BstV2I GAAGAC 2 cut(s) 791, 1856
BstX2I RGATCY 1 cut(s) 932
BstXI CCANNNNNNTGG 2 cut(s) 641, 1245
BstYI RGATCY 1 cut(s) 932
BsuRI GGCC 9 cut(s) 251, 278, 335, 645, 659, 810, 1456, 1560, 1605
BtgZI GCGATG 2 cut(s) 313, 689
BtrI CACGTC 1 cut(s) 814
BtsCI GGATG 5 cut(s) 991, 1021, 1122, 1405, 1918
BtsI GCAGTG 3 cut(s) 148, 380, 1389
BtsIMutI CAGTG 8 cut(s) 148, 380, 567, 964, 1372, 1389, 1656, 1860
Cac8I GCNNGC 8 cut(s) 130, 337, 498, 1228, 1265, 1486, 1562, 1632
CaiI CAGNNNCTG 1 cut(s) 1475
CfoI GCGC 5 cut(s) 76, 88, 134, 298, 320
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 3 cut(s) 189, 205, 1455
CseI GACGC 4 cut(s) 129, 302, 646, 1259
Csp6I GTAC 2 cut(s) 403, 1719
CspCI CAANNNNNGTGG 2 cut(s) 556, 591
CviAII CATG 8 cut(s) 400, 560, 785, 866, 1166, 1264, 1643, 1919
CviQI GTAC 2 cut(s) 403, 1719
DdeI CTNAG 9 cut(s) 209, 258, 314, 429, 749, 936, 1191, 1416, 1877
DinI GGCGCC 1 cut(s) 133
DpnI GATC 9 cut(s) 196, 282, 413, 900, 934, 1541, 1787, 1859, 1934
DpnII GATC 9 cut(s) 194, 280, 411, 898, 932, 1539, 1785, 1857, 1932
EaeI YGGCCR 6 cut(s) 249, 276, 643, 808, 1558, 1603
Eam1104I CTCTTC 3 cut(s) 42, 1043, 1562
EarI CTCTTC 3 cut(s) 42, 1043, 1562
EciI GGCGGA 1 cut(s) 43
Ecl136II GAGCTC 2 cut(s) 265, 1664
Eco130I CCWWGG 1 cut(s) 1797
Eco147I AGGCCT 1 cut(s) 335
Eco24I GRGCYC 2 cut(s) 267, 1666
Eco31I GGTCTC 1 cut(s) 1508
Eco47I GGWCC 2 cut(s) 189, 205
Eco47III AGCGCT 2 cut(s) 75, 319
Eco53kI GAGCTC 2 cut(s) 265, 1664
Eco57I CTGAAG 7 cut(s) 383, 752, 1067, 1497, 1586, 1868, 1908
Eco88I CYCGRG 2 cut(s) 184, 902
EcoICRI GAGCTC 2 cut(s) 265, 1664
EcoO109I RGGNCCY 3 cut(s) 189, 205, 1455
EcoRII CCWGG 6 cut(s) 96, 107, 708, 1303, 1515, 1895
EcoT14I CCWWGG 1 cut(s) 1797
EcoT22I ATGCAT 3 cut(s) 312, 470, 1109
EcoT38I GRGCYC 2 cut(s) 267, 1666
EgeI GGCGCC 1 cut(s) 133
EheI GGCGCC 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 1797
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 8 cut(s) 403, 563, 788, 869, 1169, 1267, 1646, 1922
FaqI GGGAC 2 cut(s) 28, 1106
FatI CATG 8 cut(s) 399, 559, 784, 865, 1165, 1263, 1642, 1918
FauI CCCGC 3 cut(s) 54, 240, 638
FbaI TGATCA 1 cut(s) 1785
FokI GGATG 5 cut(s) 998, 1028, 1109, 1412, 1925
FriOI GRGCYC 2 cut(s) 267, 1666
FspBI CTAG 7 cut(s) 462, 515, 594, 1055, 1227, 1431, 1452
GlaI GCGC 5 cut(s) 75, 87, 133, 297, 319
GsuI CTGGAG 3 cut(s) 506, 1341, 1554
HaeII RGCGCY 3 cut(s) 77, 135, 321
HaeIII GGCC 9 cut(s) 251, 278, 335, 645, 659, 810, 1456, 1560, 1605
HapII CCGG 3 cut(s) 129, 135, 198
HgaI GACGC 4 cut(s) 129, 302, 646, 1259
HhaI GCGC 5 cut(s) 76, 88, 134, 298, 320
Hin1I GRCGYC 2 cut(s) 132, 140
Hin1II CATG 8 cut(s) 403, 563, 788, 869, 1169, 1267, 1646, 1922
Hin6I GCGC 5 cut(s) 74, 86, 132, 296, 318
HinP1I GCGC 5 cut(s) 74, 86, 132, 296, 318
HincII GTYRAC 2 cut(s) 105, 117
HindII GTYRAC 2 cut(s) 105, 117
HinfI GANTC 5 cut(s) 518, 648, 1010, 1744, 1862
HpaII CCGG 3 cut(s) 129, 135, 198
HphI GGTGA 2 cut(s) 556, 1505
Hpy166II GTNNAC 4 cut(s) 105, 117, 974, 1409
Hpy188I TCNGA 6 cut(s) 430, 750, 832, 937, 1015, 1257
Hpy188III TCNNGA 9 cut(s) 260, 329, 462, 682, 728, 770, 902, 1007, 1709
Hpy8I GTNNAC 4 cut(s) 105, 117, 974, 1409
Hpy99I CGWCG 3 cut(s) 145, 257, 818
HpyAV CCTTC 8 cut(s) 350, 358, 752, 776, 1472, 1768, 1828, 1832
HpyCH4III ACNGT 7 cut(s) 121, 571, 968, 1447, 1651, 1718, 1855
HpyCH4IV ACGT 2 cut(s) 813, 1809
HpyF10VI GCNNNNNNNGC 8 cut(s) 73, 159, 816, 1421, 1481, 1517, 1631, 1892
HpyF3I CTNAG 9 cut(s) 209, 258, 314, 429, 749, 936, 1191, 1416, 1877
HpySE526I ACGT 2 cut(s) 813, 1809
Hsp92I GRCGYC 2 cut(s) 132, 140
Hsp92II CATG 8 cut(s) 403, 563, 788, 869, 1169, 1267, 1646, 1922
HspAI GCGC 5 cut(s) 74, 86, 132, 296, 318
KasI GGCGCC 1 cut(s) 131
KpnI GGTACC 1 cut(s) 1722
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 1785
Kzo9I GATC 9 cut(s) 194, 280, 411, 898, 932, 1539, 1785, 1857, 1932
LguI GCTCTTC 2 cut(s) 42, 1562
LmnI GCTCC 6 cut(s) 33, 262, 367, 511, 1392, 1661
Lsp1109I GCAGC 8 cut(s) 174, 247, 382, 1141, 1235, 1254, 1484, 1745
LweI GCATC 7 cut(s) 319, 455, 477, 712, 979, 1131, 1882
MaeI CTAG 7 cut(s) 462, 515, 594, 1055, 1227, 1431, 1452
MaeII ACGT 2 cut(s) 813, 1809
MaeIII GTNAC 7 cut(s) 562, 598, 674, 1645, 1651, 1667, 1753
MalI GATC 9 cut(s) 196, 282, 413, 900, 934, 1541, 1787, 1859, 1934
MbiI CCGCTC 2 cut(s) 49, 178
MboI GATC 9 cut(s) 194, 280, 411, 898, 932, 1539, 1785, 1857, 1932
MflI RGATCY 1 cut(s) 932
MhlI GDGCHC 4 cut(s) 267, 976, 1389, 1666
MlsI TGGCCA 3 cut(s) 810, 1560, 1605
MluNI TGGCCA 3 cut(s) 810, 1560, 1605
Mly113I GGCGCC 1 cut(s) 132
MlyI GAGTC 5 cut(s) 527, 657, 1019, 1753, 1856
MmeI TCCRAC 3 cut(s) 993, 1318, 1642
Mox20I TGGCCA 3 cut(s) 810, 1560, 1605
Mph1103I ATGCAT 3 cut(s) 312, 470, 1109
MroNI GCCGGC 1 cut(s) 128
MscI TGGCCA 3 cut(s) 810, 1560, 1605
MseI TTAA 5 cut(s) 953, 957, 1350, 1739, 1948
MslI CAYNNNNRTG 2 cut(s) 969, 1643
Msp20I TGGCCA 3 cut(s) 810, 1560, 1605
MspA1I CMGCKG 4 cut(s) 238, 1241, 1475, 1564
MspI CCGG 3 cut(s) 129, 135, 198
MspR9I CCNGG 7 cut(s) 98, 109, 136, 710, 1305, 1517, 1897
Mva1269I GAATGC 2 cut(s) 1109, 1870
MvaI CCWGG 6 cut(s) 98, 109, 710, 1305, 1517, 1897
MvnI CGCG 4 cut(s) 67, 86, 296, 298
MwoI GCNNNNNNNGC 8 cut(s) 73, 159, 816, 1421, 1481, 1517, 1631, 1892
NaeI GCCGGC 1 cut(s) 130
NarI GGCGCC 1 cut(s) 132
NciI CCSGG 1 cut(s) 136
NdeII GATC 9 cut(s) 194, 280, 411, 898, 932, 1539, 1785, 1857, 1932
NgoMIV GCCGGC 1 cut(s) 128
NheI GCTAGC 1 cut(s) 1226
NlaIII CATG 8 cut(s) 403, 563, 788, 869, 1169, 1267, 1646, 1922
NlaIV GGNNCC 6 cut(s) 133, 191, 450, 1457, 1720, 1779
NmeAIII GCCGAG 1 cut(s) 671
NmuCI GTSAC 4 cut(s) 562, 598, 1645, 1651
NsiI ATGCAT 3 cut(s) 312, 470, 1109
NspI RCATGY 3 cut(s) 563, 1169, 1267
OliI CACNNNNGTG 2 cut(s) 969, 1643
PacI TTAATTAA 1 cut(s) 957
PaeI GCATGC 1 cut(s) 1267
PceI AGGCCT 1 cut(s) 335
PciI ACATGT 1 cut(s) 559
PciSI GCTCTTC 2 cut(s) 42, 1562
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PctI GAATGC 2 cut(s) 1109, 1870
PdiI GCCGGC 1 cut(s) 130
PflMI CCANNNNNTGG 1 cut(s) 1396
PleI GAGTC 5 cut(s) 526, 656, 1018, 1752, 1856
PluTI GGCGCC 1 cut(s) 135
PpsI GAGTC 5 cut(s) 526, 656, 1018, 1752, 1856
PpuMI RGGWCCY 2 cut(s) 189, 205
PscI ACATGT 1 cut(s) 559
Psp124BI GAGCTC 2 cut(s) 267, 1666
Psp1406I AACGTT 1 cut(s) 1809
Psp5II RGGWCCY 2 cut(s) 189, 205
Psp6I CCWGG 6 cut(s) 96, 107, 708, 1303, 1515, 1895
PspGI CCWGG 6 cut(s) 96, 107, 708, 1303, 1515, 1895
PspN4I GGNNCC 6 cut(s) 133, 191, 450, 1457, 1720, 1779
PspPI GGNCC 3 cut(s) 189, 205, 1455
PspPPI RGGWCCY 2 cut(s) 189, 205
PstI CTGCAG 1 cut(s) 1131
PstNI CAGNNNCTG 1 cut(s) 1475
PsuI RGATCY 1 cut(s) 932
PvuII CAGCTG 4 cut(s) 238, 1241, 1475, 1564
RsaI GTAC 2 cut(s) 404, 1720
RsaNI GTAC 2 cut(s) 403, 1719
RseI CAYNNNNRTG 2 cut(s) 969, 1643
SacI GAGCTC 2 cut(s) 267, 1666
SapI GCTCTTC 2 cut(s) 42, 1562
SaqAI TTAA 5 cut(s) 953, 957, 1350, 1739, 1948
Sau3AI GATC 9 cut(s) 194, 280, 411, 898, 932, 1539, 1785, 1857, 1932
Sau96I GGNCC 3 cut(s) 189, 205, 1455
SchI GAGTC 5 cut(s) 527, 657, 1019, 1753, 1856
ScrFI CCNGG 7 cut(s) 98, 109, 136, 710, 1305, 1517, 1897
SduI GDGCHC 4 cut(s) 267, 976, 1389, 1666
SfaNI GCATC 7 cut(s) 319, 455, 477, 712, 979, 1131, 1882
SfcI CTRYAG 3 cut(s) 911, 1127, 1425
SfoI GGCGCC 1 cut(s) 133
SinI GGWCC 2 cut(s) 189, 205
SmiMI CAYNNNNRTG 2 cut(s) 969, 1643
SmlI CTYRAG 3 cut(s) 266, 580, 1007
SmoI CTYRAG 3 cut(s) 266, 580, 1007
SphI GCATGC 1 cut(s) 1267
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 8 cut(s) 47, 54, 65, 112, 176, 233, 631, 992
SspDI GGCGCC 1 cut(s) 131
SspMI CTAG 7 cut(s) 462, 515, 594, 1055, 1227, 1431, 1452
SstI GAGCTC 2 cut(s) 267, 1666
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 7 cut(s) 96, 107, 134, 708, 1303, 1515, 1895
StyI CCWWGG 1 cut(s) 1797
TaaI ACNGT 7 cut(s) 121, 571, 968, 1447, 1651, 1718, 1855
TaiI ACGT 2 cut(s) 816, 1812
TaqI TCGA 6 cut(s) 143, 180, 1542, 1674, 1710, 1860
TauI GCSGC 2 cut(s) 67, 178
Tru1I TTAA 5 cut(s) 953, 957, 1350, 1739, 1948
Tru9I TTAA 5 cut(s) 953, 957, 1350, 1739, 1948
TscAI CASTG 8 cut(s) 155, 380, 574, 971, 1372, 1389, 1656, 1860
TseFI GTSAC 4 cut(s) 562, 598, 1645, 1651
TseI GCWGC 8 cut(s) 162, 235, 370, 1129, 1223, 1267, 1472, 1733
Tsp45I GTSAC 4 cut(s) 562, 598, 1645, 1651
TspDTI ATGAA 5 cut(s) 332, 734, 1023, 1032, 1716
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 8 cut(s) 155, 380, 574, 971, 1372, 1389, 1656, 1860
Van91I CCANNNNNTGG 1 cut(s) 1396
VneI GTGCAC 1 cut(s) 972
VpaK11BI GGWCC 2 cut(s) 189, 205
XapI RAATTY 6 cut(s) 589, 821, 949, 1251, 1289, 1676
XbaI TCTAGA 1 cut(s) 461
XceI RCATGY 3 cut(s) 563, 1169, 1267
XcmI CCANNNNNNNNNTGG 3 cut(s) 529, 1397, 1771
XspI CTAG 7 cut(s) 462, 515, 594, 1055, 1227, 1431, 1452
Zsp2I ATGCAT 3 cut(s) 312, 470, 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.