MD10G1156700.v1.1

Protein of unknown function (DUF760)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010353
Physical Location & Seq
Forward (+)
24544185 .. 24546551
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1156700.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGACCATTGTCTCGCCCTTTCATCCCTCAAGCTCCGCGTAACCCCCGCCTTCACTCCTTCCCGCCCCGCCGTCCAATCGCGGACCGCCAATCGGTTCTTCAGGCCGTTGACCGTCGTCGCCGGAGCCGGGGCGAGCCACTGCGAGTTCAGCAGCCTCAACTCGCCGCTCGACCCGCGGACCCGACCCGGAAAGGACCTCTGCACCGTCTTGCAGAATCATCCGCAGCTGTTCCATCTTGCGGTCGCTCAGGAGTTGAAGAAGTTGGCCGATGATCGGGAACTCGCTCTCTCGCGTGCGTCTCTCAGCGCCGCCTCTCATGAGGCCTGCCTTCATAGGAGGATTGCACAACTGAAGGAGCAGGAGTGTGAGACTGTTGTCGAAGATGTCATGTACCTTTTAGTCTTTTACAAGTTTTCTGAGATCAAAGTTCATTTGGTTCCTAAGCTTTCTAGATGCATCTACAATGCGAGACTGGAGATATGGCCTTCAAAGGATTGGGAACTAGAGTCCCTTTACAGCGTGGACGTTTTGGAGATGATAAGGGAACATGTAAGCACAGTCATTGGCTTGAAGGCAAATTCTAGCGTCACAGACAATTGGGCAGTGACAACGATCACACGTCAAACACTTGGTCAAGCATATGTGGCTTCCATCTTATACGGTTACTTTCTGAAATCTGCCTCATTGAGACACAGCCTGGACCGCATTCTAACTCTGGAAAGCCAAGACCTCCATATGAGTCACCGAACCTCCCTTCCGTTTCAGGAAATGTGTCCTAATGAAAACAAAAATCTTCTCTTTGGCCGCATTGGCAACATTCAAACGAAGTTTCAAGGGCCAAGTAGGCAGGAGAAGACACATGGAAAGTTGAGATGTTACGTGATGGGGTTTGATCCTAATACACTTCAGAGATGCGCAAAACTGAGATCCAAGGTGGCTGTGAATTTAATTAAGAATCACTGTTGTGCACTCTTCGGCGATGATGGGAGCATGGGTTCGCCTGAGACCGATGAAGTTATCTCAACTTCATATTCAAGTCTGAAGAGGCTAGTTTTGGAGGCTGTCGCCTTTGGTTCGTTCCTCTGGGACACTGAAGAATGCATTGAGACAATATATGAGCTTAAGTCGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF760

Protein Analysis

379

Amino Acids

42.6

Weight (kDa)

8.21

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF760 PF05542 111 - 238 7.7e-17 Protein of unknown function (DUF760)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07310 AT3G07310 AT5G48590
fragaria_vesca FvH4_2g18230 FvH4_2g18230 FvH4_2g18230
malus_domestica MD05G1166700.v1.1 MD10G1156700.v1.1
prunus_persica Prupe.8G195000_v2.0.a1
pyrus_communis pycom05g15550 pycom10g13490
rosa_chinensis RchiOBHm_Chr6g0283601
rosa_laevigata RLG00000012813
rosa_multiflora Rmu_co8416891.1_g000001
rosa_roxburghii Rroxscaffold_7G00185360
rosa_rugosa Rorug06G0156000
rosa_samantha Rh6AG267800 Rh6BG270300 Rh6CG270100 Rh6DG263800
rosa_wichuraiana Rw6G023200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 919
AccBSI CCGCTC 1 cut(s) 169
AccII CGCG 4 cut(s) 39, 82, 178, 295
AclWI GGATC 2 cut(s) 890, 924
AcoI YGGCCR 2 cut(s) 267, 805
AcsI RAATTY 2 cut(s) 580, 946
AcuI CTGAAG 5 cut(s) 85, 374, 893, 1064, 1116
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 4 cut(s) 93, 129, 241, 276
AflII CTTAAG 1 cut(s) 1124
AflIII ACRYGT 2 cut(s) 550, 620
AgsI TTSAA 6 cut(s) 259, 492, 574, 824, 836, 1038
AjiI CACGTC 1 cut(s) 623
AjnI CCWGG 1 cut(s) 699
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AleI CACNNNNGTG 1 cut(s) 966
AloI GAACNNNNNNTCC 4 cut(s) 742, 774, 982, 1014
AluBI AGCT 4 cut(s) 34, 229, 448, 1123
AluI AGCT 4 cut(s) 34, 229, 448, 1123
Alw21I GWGCWC 1 cut(s) 973
Alw26I GTCTC 7 cut(s) 17, 306, 365, 466, 685, 1001, 1103
Alw44I GTGCAC 1 cut(s) 969
AlwI GGATC 2 cut(s) 890, 924
AoxI GGCC 6 cut(s) 104, 267, 324, 485, 805, 839
ApaLI GTGCAC 1 cut(s) 969
ApeKI GCWGC 2 cut(s) 153, 226
ApoI RAATTY 2 cut(s) 580, 946
ArsI GACNNNNNNTTYG 2 cut(s) 619, 651
AspLEI GCGC 2 cut(s) 311, 920
AspS9I GGNCC 6 cut(s) 4, 84, 180, 196, 703, 839
AsuC2I CCSGG 2 cut(s) 130, 189
AsuHPI GGTGA 1 cut(s) 737
AvaII GGWCC 5 cut(s) 4, 84, 180, 196, 703
BaeGI GKGCMC 1 cut(s) 973
BbsI GAAGAC 1 cut(s) 863
Bbv12I GWGCWC 1 cut(s) 973
BbvI GCAGC 2 cut(s) 165, 238
BccI CCATC 4 cut(s) 243, 662, 880, 980
BceAI ACGGC 2 cut(s) 56, 91
BciT130I CCWGG 1 cut(s) 701
BcnI CCSGG 2 cut(s) 130, 189
BcoDI GTCTC 7 cut(s) 17, 306, 365, 466, 685, 1001, 1103
BfaI CTAG 4 cut(s) 453, 506, 585, 1052
BfoI RGCGCY 1 cut(s) 312
BfrI CTTAAG 1 cut(s) 1124
BglI GCCNNNNNGGC 2 cut(s) 813, 847
BisI GCNGC 5 cut(s) 154, 167, 227, 312, 808
BlsI GCNGC 5 cut(s) 155, 168, 228, 313, 809
Bme1390I CCNGG 3 cut(s) 130, 189, 701
Bme18I GGWCC 5 cut(s) 4, 84, 180, 196, 703
BmgBI CACGTC 1 cut(s) 623
BmgT120I GGNCC 6 cut(s) 4, 84, 180, 196, 703, 839
BmiI GGNNCC 3 cut(s) 127, 182, 441
BmrFI CCNGG 3 cut(s) 130, 189, 701
BmsI GCATC 3 cut(s) 446, 468, 905
BoxI GACNNNNGTC 3 cut(s) 9, 116, 377
BpiI GAAGAC 1 cut(s) 863
BpmI CTGGAG 1 cut(s) 497
Bpu10I CCTNAGC 2 cut(s) 249, 444
BpuEI CTTGAG 1 cut(s) 14
BpuMI CCSGG 2 cut(s) 130, 189
BsaAI YACGTR 1 cut(s) 883
BsaI GGTCTC 1 cut(s) 1001
BsaJI CCNNGG 3 cut(s) 129, 176, 933
BsaXI ACNNNNNCTCC 6 cut(s) 350, 380, 737, 767, 982, 1012
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 129, 241, 276
Bse1I ACTGG 1 cut(s) 480
BseBI CCWGG 1 cut(s) 701
BseDI CCNNGG 3 cut(s) 129, 176, 933
BseGI GGATG 2 cut(s) 23, 220
BseLI CCNNNNNNNGG 4 cut(s) 93, 129, 241, 276
BseMII CTCAG 5 cut(s) 263, 319, 411, 917, 996
BseNI ACTGG 1 cut(s) 480
BseSI GKGCMC 1 cut(s) 973
BseXI GCAGC 2 cut(s) 165, 238
BsgI GTGCAG 1 cut(s) 187
Bsh1236I CGCG 4 cut(s) 39, 82, 178, 295
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 6 cut(s) 106, 269, 326, 487, 807, 841
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 973
BsiSI CCGG 3 cut(s) 123, 129, 189
BslFI GGGAC 2 cut(s) 496, 1103
BslI CCNNNNNNNGG 4 cut(s) 93, 129, 241, 276
BsmAI GTCTC 7 cut(s) 17, 306, 365, 466, 685, 1001, 1103
BsmBI CGTCTC 1 cut(s) 306
BsmFI GGGAC 2 cut(s) 496, 1103
BsmI GAATGC 2 cut(s) 708, 1106
BsnI GGCC 6 cut(s) 106, 269, 326, 487, 807, 841
Bso31I GGTCTC 1 cut(s) 1001
Bsp1286I GDGCHC 1 cut(s) 973
Bsp143I GATC 5 cut(s) 274, 423, 615, 895, 929
BspANI GGCC 6 cut(s) 106, 269, 326, 487, 807, 841
BspCNI CTCAG 5 cut(s) 262, 318, 412, 918, 997
BspFNI CGCG 4 cut(s) 39, 82, 178, 295
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 3 cut(s) 127, 182, 441
BspPI GGATC 2 cut(s) 890, 924
BspTI CTTAAG 1 cut(s) 1124
BspTNI GGTCTC 1 cut(s) 1001
BsrBI CCGCTC 1 cut(s) 169
BsrI ACTGG 1 cut(s) 480
BssECI CCNNGG 3 cut(s) 129, 176, 933
BssMI GATC 5 cut(s) 274, 423, 615, 895, 929
BssT1I CCWWGG 1 cut(s) 933
Bst2UI CCWGG 1 cut(s) 701
Bst4CI ACNGT 6 cut(s) 115, 208, 376, 562, 665, 965
Bst6I CTCTTC 2 cut(s) 980, 1040
BstAFI CTTAAG 1 cut(s) 1124
BstBAI YACGTR 1 cut(s) 883
BstC8I GCNNGC 3 cut(s) 136, 297, 328
BstDEI CTNAG 6 cut(s) 249, 305, 420, 444, 926, 1005
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 2 cut(s) 23, 220
BstFNI CGCG 4 cut(s) 39, 82, 178, 295
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 2 cut(s) 311, 920
BstKTI GATC 5 cut(s) 277, 426, 618, 898, 932
BstMAI GTCTC 7 cut(s) 17, 306, 365, 466, 685, 1001, 1103
BstMBI GATC 5 cut(s) 274, 423, 615, 895, 929
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 6 cut(s) 150, 175, 647, 705, 813, 847
BstNI CCWGG 1 cut(s) 701
BstNSI RCATGY 1 cut(s) 554
BstPAI GACNNNNGTC 3 cut(s) 9, 116, 377
BstSCI CCNGG 3 cut(s) 128, 187, 699
BstSLI GKGCMC 1 cut(s) 973
BstUI CGCG 4 cut(s) 39, 82, 178, 295
BstV1I GCAGC 2 cut(s) 165, 238
BstV2I GAAGAC 1 cut(s) 863
BstX2I RGATCY 1 cut(s) 929
BstYI RGATCY 1 cut(s) 929
BsuRI GGCC 6 cut(s) 106, 269, 326, 487, 807, 841
BtgI CCRYGG 1 cut(s) 176
BtgZI GCGATG 1 cut(s) 996
BtrI CACGTC 1 cut(s) 623
BtsCI GGATG 2 cut(s) 23, 220
BtsI GCAGTG 2 cut(s) 139, 612
BtsIMutI CAGTG 4 cut(s) 139, 612, 961, 1092
Cac8I GCNNGC 3 cut(s) 136, 297, 328
CciI TCATGA 1 cut(s) 319
CfoI GCGC 2 cut(s) 311, 920
Cfr13I GGNCC 6 cut(s) 4, 84, 180, 196, 703, 839
Cfr42I CCGCGG 1 cut(s) 179
CpoI CGGWCCG 1 cut(s) 84
CseI GACGC 2 cut(s) 288, 577
Csp6I GTAC 1 cut(s) 394
CspI CGGWCCG 1 cut(s) 84
CviAII CATG 5 cut(s) 320, 391, 551, 863, 994
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 6 cut(s) 249, 305, 420, 444, 926, 1005
DpnI GATC 5 cut(s) 276, 425, 617, 897, 931
DpnII GATC 5 cut(s) 274, 423, 615, 895, 929
EaeI YGGCCR 2 cut(s) 267, 805
Eam1104I CTCTTC 2 cut(s) 980, 1040
EarI CTCTTC 2 cut(s) 980, 1040
Eco130I CCWWGG 1 cut(s) 933
Eco147I AGGCCT 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 1001
Eco47I GGWCC 5 cut(s) 4, 84, 180, 196, 703
Eco57I CTGAAG 5 cut(s) 85, 374, 893, 1064, 1116
EcoO109I RGGNCCY 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 699
EcoT14I CCWWGG 1 cut(s) 933
EcoT22I ATGCAT 2 cut(s) 461, 1106
ErhI CCWWGG 1 cut(s) 933
Esp3I CGTCTC 1 cut(s) 306
FaeI CATG 5 cut(s) 323, 394, 554, 866, 997
FaqI GGGAC 2 cut(s) 496, 1103
FatI CATG 5 cut(s) 319, 390, 550, 862, 993
FauI CCCGC 4 cut(s) 55, 71, 76, 183
FauNDI CATATG 2 cut(s) 643, 738
Fnu4HI GCNGC 5 cut(s) 154, 167, 227, 312, 808
FokI GGATG 2 cut(s) 10, 207
Fsp4HI GCNGC 5 cut(s) 154, 167, 227, 312, 808
FspBI CTAG 4 cut(s) 453, 506, 585, 1052
FspI TGCGCA 1 cut(s) 919
GlaI GCGC 2 cut(s) 310, 919
GluI GCNGC 5 cut(s) 154, 167, 227, 312, 808
GsuI CTGGAG 1 cut(s) 497
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 6 cut(s) 106, 269, 326, 487, 807, 841
HapII CCGG 3 cut(s) 123, 129, 189
HgaI GACGC 2 cut(s) 288, 577
HhaI GCGC 2 cut(s) 311, 920
Hin1II CATG 5 cut(s) 323, 394, 554, 866, 997
Hin6I GCGC 2 cut(s) 309, 918
HinP1I GCGC 2 cut(s) 309, 918
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 4 cut(s) 217, 509, 742, 958
HpaII CCGG 3 cut(s) 123, 129, 189
HphI GGTGA 1 cut(s) 737
Hpy166II GTNNAC 3 cut(s) 111, 526, 971
Hpy188I TCNGA 4 cut(s) 421, 675, 912, 1044
Hpy188III TCNNGA 6 cut(s) 251, 278, 320, 453, 719, 767
Hpy8I GTNNAC 3 cut(s) 111, 526, 971
Hpy99I CGWCG 2 cut(s) 119, 122
HpyAV CCTTC 7 cut(s) 61, 69, 341, 349, 498, 568, 767
HpyCH4III ACNGT 6 cut(s) 115, 208, 376, 562, 665, 965
HpyCH4IV ACGT 3 cut(s) 528, 622, 882
HpyCH4V TGCA 6 cut(s) 204, 214, 347, 459, 971, 1104
HpyF10VI GCNNNNNNNGC 6 cut(s) 150, 175, 647, 705, 813, 847
HpyF3I CTNAG 6 cut(s) 249, 305, 420, 444, 926, 1005
HpySE526I ACGT 3 cut(s) 528, 622, 882
Hsp92II CATG 5 cut(s) 323, 394, 554, 866, 997
HspAI GCGC 2 cut(s) 309, 918
KspI CCGCGG 1 cut(s) 179
Kzo9I GATC 5 cut(s) 274, 423, 615, 895, 929
LmnI GCTCC 4 cut(s) 39, 125, 358, 990
Lsp1109I GCAGC 2 cut(s) 165, 238
LweI GCATC 3 cut(s) 446, 468, 905
MaeI CTAG 4 cut(s) 453, 506, 585, 1052
MaeII ACGT 3 cut(s) 528, 622, 882
MaeIII GTNAC 6 cut(s) 40, 589, 607, 665, 743, 878
MalI GATC 5 cut(s) 276, 425, 617, 897, 931
MbiI CCGCTC 1 cut(s) 169
MboI GATC 5 cut(s) 274, 423, 615, 895, 929
MboII GAAGA 8 cut(s) 91, 271, 395, 788, 868, 967, 1057, 1109
MfeI CAATTG 1 cut(s) 598
MflI RGATCY 1 cut(s) 929
MhlI GDGCHC 1 cut(s) 973
MluCI AATT 4 cut(s) 580, 598, 946, 951
MlyI GAGTC 2 cut(s) 518, 751
Mph1103I ATGCAT 2 cut(s) 461, 1106
MseI TTAA 3 cut(s) 950, 954, 1125
MslI CAYNNNNRTG 1 cut(s) 966
MspA1I CMGCKG 2 cut(s) 178, 229
MspCI CTTAAG 1 cut(s) 1124
MspI CCGG 3 cut(s) 123, 129, 189
MspR9I CCNGG 3 cut(s) 130, 189, 701
MunI CAATTG 1 cut(s) 598
Mva1269I GAATGC 2 cut(s) 708, 1106
MvaI CCWGG 1 cut(s) 701
MvnI CGCG 4 cut(s) 39, 82, 178, 295
MwoI GCNNNNNNNGC 6 cut(s) 150, 175, 647, 705, 813, 847
NciI CCSGG 2 cut(s) 130, 189
NdeI CATATG 2 cut(s) 643, 738
NdeII GATC 5 cut(s) 274, 423, 615, 895, 929
NlaIII CATG 5 cut(s) 323, 394, 554, 866, 997
NlaIV GGNNCC 3 cut(s) 127, 182, 441
NmuCI GTSAC 3 cut(s) 589, 607, 743
NsbI TGCGCA 1 cut(s) 919
NsiI ATGCAT 2 cut(s) 461, 1106
NspI RCATGY 1 cut(s) 554
OliI CACNNNNGTG 1 cut(s) 966
PacI TTAATTAA 1 cut(s) 954
PagI TCATGA 1 cut(s) 319
PceI AGGCCT 1 cut(s) 326
PciI ACATGT 1 cut(s) 550
PctI GAATGC 2 cut(s) 708, 1106
PfeI GAWTC 2 cut(s) 217, 958
PkrI GCNGC 5 cut(s) 155, 168, 228, 313, 809
PleI GAGTC 2 cut(s) 517, 750
PpsI GAGTC 2 cut(s) 517, 750
Ppu21I YACGTR 1 cut(s) 883
PpuMI RGGWCCY 1 cut(s) 196
PscI ACATGT 1 cut(s) 550
PshAI GACNNNNGTC 3 cut(s) 9, 116, 377
Psp5II RGGWCCY 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 699
PspGI CCWGG 1 cut(s) 699
PspN4I GGNNCC 3 cut(s) 127, 182, 441
PspPI GGNCC 6 cut(s) 4, 84, 180, 196, 703, 839
PspPPI RGGWCCY 1 cut(s) 196
PsuI RGATCY 1 cut(s) 929
PvuII CAGCTG 1 cut(s) 229
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 966
Rsr2I CGGWCCG 1 cut(s) 84
RsrII CGGWCCG 1 cut(s) 84
SacII CCGCGG 1 cut(s) 179
SaqAI TTAA 3 cut(s) 950, 954, 1125
SatI GCNGC 5 cut(s) 154, 167, 227, 312, 808
Sau3AI GATC 5 cut(s) 274, 423, 615, 895, 929
Sau96I GGNCC 6 cut(s) 4, 84, 180, 196, 703, 839
SchI GAGTC 2 cut(s) 518, 751
ScrFI CCNGG 3 cut(s) 130, 189, 701
SduI GDGCHC 1 cut(s) 973
SfaNI GCATC 3 cut(s) 446, 468, 905
Sfr303I CCGCGG 1 cut(s) 179
SgrBI CCGCGG 1 cut(s) 179
SinI GGWCC 5 cut(s) 4, 84, 180, 196, 703
SmiMI CAYNNNNRTG 1 cut(s) 966
SmlI CTYRAG 2 cut(s) 29, 1124
SmoI CTYRAG 2 cut(s) 29, 1124
Sse9I AATT 4 cut(s) 580, 598, 946, 951
SseBI AGGCCT 1 cut(s) 326
SspMI CTAG 4 cut(s) 453, 506, 585, 1052
StuI AGGCCT 1 cut(s) 326
StyD4I CCNGG 3 cut(s) 128, 187, 699
StyI CCWWGG 1 cut(s) 933
TaaI ACNGT 6 cut(s) 115, 208, 376, 562, 665, 965
TaiI ACGT 3 cut(s) 531, 625, 885
TaqI TCGA 3 cut(s) 171, 381, 1130
TaqII GACCGA 1 cut(s) 1025
TasI AATT 4 cut(s) 580, 598, 946, 951
TauI GCSGC 3 cut(s) 169, 314, 810
TfiI GAWTC 2 cut(s) 217, 958
Tru1I TTAA 3 cut(s) 950, 954, 1125
Tru9I TTAA 3 cut(s) 950, 954, 1125
TscAI CASTG 4 cut(s) 146, 612, 968, 1099
TseFI GTSAC 3 cut(s) 589, 607, 743
TseI GCWGC 2 cut(s) 153, 226
Tsp45I GTSAC 3 cut(s) 589, 607, 743
TspDTI ATGAA 6 cut(s) 12, 323, 422, 798, 1020, 1029
TspGWI ACGGA 1 cut(s) 750
TspRI CASTG 4 cut(s) 146, 612, 968, 1099
Vha464I CTTAAG 1 cut(s) 1124
VneI GTGCAC 1 cut(s) 969
VpaK11BI GGWCC 5 cut(s) 4, 84, 180, 196, 703
XapI RAATTY 2 cut(s) 580, 946
XbaI TCTAGA 1 cut(s) 452
XceI RCATGY 1 cut(s) 554
XspI CTAG 4 cut(s) 453, 506, 585, 1052
Zsp2I ATGCAT 2 cut(s) 461, 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.